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Viability assessment transplantation

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https://www.readbyqxmd.com/read/28914392/alginate-hyaluronic-acid-hydrogel-delivery-system-characteristics-regulate-the-differentiation-of-periodontal-ligament-stem-cells-toward-chondrogenic-lineage
#1
Sahar Ansari, Ivana M Diniz, Chider Chen, Tara Aghaloo, Benjamin M Wu, Songtao Shi, Alireza Moshaverinia
Cartilage tissue regeneration often presents a challenging clinical situation. Recently, it has been shown that Periodontal Ligament Stem Cells (PDLSCs) possess high chondrogenic differentiation capacity. In this study, we developed a stem cell delivery system based on alginate/hyaluronic acid (HA) loaded with TGF-β1 ligand, encapsulating PDLSCs; and investigated the chondrogenic differentiation of encapsulated cells in alginate/HA hydrogel microspheres in vitro and in vivo. The results showed that PDLSCs, as well as human bone marrow mesenchymal stem cells (hBMMSCs), as the positive control, were stained positive for both toluidine blue and alcian blue staining, while exhibiting high levels of gene expression related to chondrogenesis (Col II, Aggrecan and Sox-9), as assessed via qPCR...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28901972/normothermic-machine-perfusion-of-the-kidney
#2
Annemarie Weissenbacher, James Hunter
PURPOSE OF REVIEW: Normothermic machine perfusion (NMP) is a preservation method that is generating increasing interest. The aim of this review is to summarise the current status of NMP in regards of kidney viability assessment, reducing organ damage and improving transplant logistics. RECENT FINDINGS: The results of recent large animal experiments and clinical trials show that continuous prolonged normothermic ex-vivo kidney perfusion appears better than a brief period of NMP after static cold storage in terms of renal injury and function...
September 8, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28889066/effect-of-lentivirus-mediated-gene-silencing-targeting-toll-like-receptor-2-on-corneal-allograft-transplantation-in-rats
#3
Lang Bai, Weiyi Liang, Minting Chen, Yacouba Cissé, Jing Liu, Yaru Su, Jian Yu, Qiong Liu
AIM: The present work aims to assess the effectiveness of lentiviral vector (LV) mediated Toll-like receptor 2 (TLR2) gene silencing in the survival of transplanted corneal allografts, against immune rejection, in rats. METHODS: LV mediated TLR2 small interference RNA (SiRNA) with enhanced green fluorescent protein (eGFP) [LV-TLR2-siRNA-eGFP] was realised and transfected to both rat corneal epithelial (EC) and stromal cells (SC). Multiplicity of infection (MOI) was optimized for transfection efficiency using flow cytometric (FCM) analysis...
September 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28887978/neuro-differentiated-ntera2-cancer-stem-cells-encapsulated-in-alginate-beads-first-evidence-of-biological-functionality
#4
Ilaria Cacciotti, Claudia Ceci, Alessandra Bianco, Giuseppa Pistritto
The present communication investigates an application of alginate encapsulation technology to the differentiation of the embryonic cancer stem NTera2 cells (NT2) into dopamine-producing cells. The encapsulation of cells in polymeric beads allows their immune isolation and makes them eligible for transplantation, thus representing a promising biotech tool for the delivery of biologically active compounds to the brain. The polysaccharide alginate is one of the most commonly used material for this procedure since it is well tolerated by various tissues, including the brain...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887865/plasma-rich-plasma-the-ultimate-secret-for-youthful-skin-elixir-and-hair-growth-triggering
#5
REVIEW
Ebtisam Elghblawi
The clinical application of platelet-rich plasma (PRP) is based on the increase in the concentration of growth factors that are released from alpha-granule of the concentrated platelets and in the secretion of proteins which are able to capitalize on the healing process at the cellular level. It has been invented to restore the natural beauty by starting the natural rejuvenation process of the skin and aiming to make it function as a younger one and to keep the skin youthful and maintain it. Besides that, it is also emerged to include hairs as a new injectable procedure to enable stimulating hair growth locally and topically; preventing its fall; improving hair shaft, hair stem, and its caliber; increasing its shine, vitality, and pliability; and declining hair splitting and breakage...
September 8, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28884834/transplantation-of-hypoxic-preconditioned-neural-stem-cells-benefits-functional-recovery-via-enhancing-neurotrophic-secretion-after-spinal-cord-injury-in-rats
#6
Wei-Li Fan, Peng Liu, Guan Wang, Jung-Ang Pu, Xin Xue, Jian-Hua Zhao
Spinal cord injury (SCI) is a debilitating, costly, and common pathological condition that affects the function of central nervous system (CNS). To date, there are few promising therapeutic strategies available for SCI. To look for a suitable therapeutic strategy, we have developed a sublethal hypoxic preconditioning procedure using Fluorescence-activated cell sorting (FACS) analysis, LDH releasing and cell viability assays in vitro. Meanwhile, we have examined the benefits of neural stem cells (NSCs) transplantation prior to hypoxic preconditioning on functional recovery and potential mechanism via MRI screening, H&E and Nissl staining, immunofluorescence staining and Elisa assays...
September 8, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28877041/sodium-mri-a-new-frontier-in-imaging-in-nephrology
#7
Susan Francis, Charlotte E Buchanan, Ben Prestwich, Maarten W Taal
PURPOSE OF REVIEW: This review focuses on the recent technological advances in quantitative sodium (Na) MRI to provide a noninvasive measure of tissue viability for use in clinical studies of patients with kidney disease. Na MRI is the only noninvasive imaging technique that allows for the absolute spatial quantification of tissue sodium concentration (TSC), providing assessment of the corticomedullary sodium gradient (CMSG) in the kidney, and allowing measures of TSC in the skin and muscle...
September 4, 2017: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/28873283/in-vivo-like-culture-conditions-in-a-bioreactor-facilitate-improved-tissue-quality-in-corneal-storage
#8
Richard Schmid, Ioana-Sandra Tarau, Angela Rossi, Stefan Leonhardt, Thomas Schwarz, Sebastian Schuerlein, Christian Lotz, Jan Hansmann
The cornea is the most-transplanted tissue worldwide. However, the availability and quality of grafts are limited due to the current methods of corneal storage. In this study, a dynamic bioreactor system is employed to enable the control of intraocular pressure and the culture at the air-liquid interface. Thereby, in vivo-like storage conditions are achieved. Different media combinations for endothelium and epithelium are tested in standard and dynamic conditions to enhance the viability of the tissue. In contrast to culture conditions used in eye banks, the combination of the bioreactor and biochrom medium 1 allows to preserve the corneal endothelium and the epithelium...
September 5, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28832685/oxygen-environment-and-islet-size-are-the-primary-limiting-factors-of-isolated-pancreatic-islet-survival
#9
Hirotake Komatsu, Colin Cook, Chia-Hao Wang, Leonard Medrano, Henry Lin, Fouad Kandeel, Yu-Chong Tai, Yoko Mullen
BACKGROUND: Type 1 diabetes is an autoimmune disease that destroys insulin-producing beta cells in the pancreas. Pancreatic islet transplantation could be an effective treatment option for type 1 diabetes once several issues are resolved, including donor shortage, prevention of islet necrosis and loss in pre- and post-transplantation, and optimization of immunosuppression. This study seeks to determine the cause of necrotic loss of isolated islets to improve transplant efficiency. METHODOLOGY: The oxygen tension inside isolated human islets of different sizes was simulated under varying oxygen environments using a computational in silico model...
2017: PloS One
https://www.readbyqxmd.com/read/28831719/petersen-s-hernia-after-living-donor-liver-transplantation
#10
Sodai Sakamoto, Ryoichi Goto, Norio Kawamura, Yasuyuki Koshizuka, Masaaki Watanabe, Minoru Ota, Tomomi Suzuki, Daisuke Abo, Kenichiro Yamashita, Toshiya Kamiyama, Akinobu Taketomi, Tsuyoshi Shimamura
BACKGROUND: Hepaticojejunostomy may be used for biliary reconstruction in certain cases of liver transplantation. In this occasion, Roux-en-Y biliary reconstruction is predominantly performed. Petersen's hernia is an internal hernia that can occur after Roux-en-Y reconstruction, and it may lead to extensive ischemic changes affecting incarcerated portions of the small bowel or Roux limb resulting in severe complications with a poor prognosis. CASE PRESENTATION: The present case was a 44-year-old male who underwent living donor liver transplantation (LDLT) for familial amyloid polyneuropathy and in whom biliary reconstruction was performed with Roux-en-Y hepaticojejunostomy...
August 23, 2017: Surgical Case Reports
https://www.readbyqxmd.com/read/28811932/two-novel-direct-spio-labels-and-in%C3%A2-vivo-mri-detection-of-labeled-cells-after-acute-myocardial-infarct
#11
Riikka M Korpi, Kirsi Alestalo, Timo Ruuska, Eveliina Lammentausta, Ronald Borra, Fredrik Yannopoulos, Siri Lehtonen, Jarkko T Korpi, Elisa Lappi-Blanco, Vesa Anttila, Petri Lehenkari, Tatu Juvonen, Roberto Blanco Sequieros
BACKGROUND: Acute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. Cellular decay due hypoxia requires rapid and validated methods for possible therapeutic cell transplantation. PURPOSE: To develop direct and rapid superparamagnetic iron oxide (SPIO) cell label for a large-animal model and to assess in vivo cell targeting by magnetic resonance imaging (MRI) in an experimental AMI model. MATERIAL AND METHODS: Bone marrow mononuclear cells (BMMNCs) were labeled with SPIO particles using two novel direct labeling methods (rotating incubation method and electroporation)...
August 2017: Acta Radiologica Open
https://www.readbyqxmd.com/read/28800845/assessment-of-stability-of-cd34-cell-products-enriched-by-immunoselection-from-peripheral-blood-mononuclear-cells-during-refrigerated-storage
#12
Metka Krasna, Elvira Malicev, Jasmina Ziva Rozman, Bojan Vrtovec
Durable engraftment of transplanted CD34+ cells largely depends on the quality of the cell product. Limited data are currently available about extended storage of immunoselected CD34+ cells. The aim of our study was to assess the stability of CD34+ cell product with the cells stored in high concentration (80×10(6) in 6mL) in small bags intended for cell implantation. Cell products were prepared by leukapheresis and immunoselection (Clinimacs(plus) procedure) from 13 patients with chronic dilated cardiomyopathy...
July 22, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28798841/microfluidic-engineering-of-neural-stem-cell-niches-for-fate-determination
#13
Yachen Wang, Jingyun Ma, Na Li, Liang Wang, Liming Shen, Yu Sun, Yajun Wang, Jingyuan Zhao, Wenjuan Wei, Yan Ren, Jing Liu
Neural stem cell (NSC) transplantation has great therapeutic potential for neurodegenerative diseases and central nervous system injuries. Successful NSC replacement therapy requires precise control over the cellular behaviors. However, the regulation of NSC fate is largely unclear, which severely restricts the potential clinical applications. To develop an effective model, we designed an assembled microfluidic system to engineer NSC niches and assessed the effects of various culture conditions on NSC fate determination...
January 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28793833/synergistic-therapeutic-effect-of-three-dimensional-stem-cell-clusters-and-angiopoietin-1-on-promoting-vascular-regeneration-in-ischemic-region
#14
Jungmi Kang, Jeong-Kee Yoon, Seung Ja Oh, Byung-Soo Kim, Sang-Heon Kim
Peripheral artery disease (PAD) is an ischemic disease characterized by reduced blood flow to the legs, feet, and hands. Human mesenchymal stem cells (MSCs) are an attractive cell source to treat PAD in regenerative medicine. However, in clinical applications, the use of adult stem cells has several limitations, such as low cell viability and low therapeutic efficiency. In the present study, we described an innovative method of culturing three-dimensional stem cell clusters (AngioclusterTM, AC), demonstrated the potential for ACs to differentiate into vascular cells, and assessed the synergistic effects of ACs and angiopoietin-1 (Ang-1) on angiogenesis in ischemic animal models...
August 10, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28780679/locoregional-therapies-for-hepatocellular-carcinoma-and-the-new-li-rads-treatment-response-algorithm
#15
Ania Kielar, Kathryn J Fowler, Sara Lewis, Vahid Yaghmai, Frank H Miller, Hooman Yarmohammadi, Charles Kim, Victoria Chernyak, Takeshi Yokoo, Jeffrey Meyer, Isabel Newton, Richard K Do
Radiologists play a central role in the assessment of patient response to locoregional therapies for hepatocellular carcinoma (HCC). The identification of viable tumor following treatment guides further management and potentially affects transplantation eligibility. Liver Imaging Reporting and Data Systems (LI-RADS) first introduced the concept of LR-treated in 2014, and a new treatment response algorithm is included in the 2017 update to assist radiologists in image interpretation of HCC after locoregional therapy...
August 5, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28768794/embryonic-stem-cell-derived-germ-cells-induce-spermatogenesis-following-transplantation-to-testis-of-adult-mouse-azoospermia-model
#16
Zohreh Makoolati, Mansoureh Movahedin, Mehdi Forouzandeh-Moghadam, Majid Naghdi, Morteza Koruji
This study aimed to (a) identify the exogenous factors that allow in vitro differentiation of mouse SSCs from embryonic stem cells (ESCs); (b) evaluate the effects of Sertoli cells on SSCs enrichment; and (c) to assess the success of transplantation using in vitro differentiated SSCs in mouse busulfan-treated azoospermia model. One-day-old embryoid body (EB) received 5 ng/ml BMP4 for 4 days, 3 µM retinoic acid (RA) in STO co-culture (Co-C) system for 7 days and subsequently co-cultured for 2 days with Sertoli cell in the presence or absence of LIF, bFGF and RA composition and in the presence of these factors in the simple culture media...
August 2, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28766693/testing-the-niche-breadth-range-size-hypothesis-habitat-specialization-vs-performance-in-australian-alpine-daisies
#17
Megan J Hirst, Philippa C Griffin, Jason P Sexton, Ary A Hoffmann
Relatively common species within a clade are expected to perform well across a wider range of conditions than their rarer relatives, yet experimental tests of this "niche-breadth-range-size" hypothesis remain surprisingly scarce. Rarity may arise due to trade-offs between specialization and performance across a wide range of environments. Here we use common garden and reciprocal transplant experiments to test the niche-breadth-range-size hypothesis, focusing on four common and three rare endemic alpine daisies (Brachyscome spp...
August 2, 2017: Ecology
https://www.readbyqxmd.com/read/28765924/mri-reveals-slow-clearance-of-dead-cell-transplants-in-mouse-forelimb-muscles
#18
Yanhui Zhang, Hongyan Zhang, Lijun Ding, Hailu Zhang, Pengli Zhang, Haizhen Jiang, Bo Tan, Zongwu Deng
A small molecule tetraazacyclododecane-1,4,7,10-tetraacetic acid (Gd‑DOTA)4‑TPP agent is used to label human mesenchymal stem cells (hMSCs) via electroporation (EP). The present study assessed the cytotoxicity of cell labeling, in addition to its effect on cell differentiation potential. There were no significant adverse effects on cell viability or differentiation induced by either EP or cellular uptake of (Gd‑DOTA)4‑TPP. Labeled live and dead hMSCs were transplanted into mouse forelimb muscles. T2‑weighted magnetic resonance imaging (MRI) was used to track the in vivo fate of the cell transplants...
October 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28743804/paracrine-effects-of-the-pluripotent-stem-cell-derived-cardiac-myocytes-salvage-the-injured-myocardium
#19
Atsushi Tachibana, Michelle R Santoso, Morteza Mahmoudi, Praveen Shukla, Lei Wang, Mihoko Bennett, Andrew B Goldstone, Mouer Wang, Masahiro Fukushi, Antje D Ebert, Y Joseph Woo, Eric Rulifson, Phillip C Yang
RATIONALE: Cardiac myocytes derived from pluripotent stem cells have demonstrated the potential to mitigate damage of the infarcted myocardium and improve left ventricular ejection fraction. However, the mechanism underlying the functional benefit is unclear. OBJECTIVE: To evaluate whether the transplantation of cardiac-lineage differentiated derivatives enhance myocardial viability and restore left ventricular ejection fraction more effectively than undifferentiated pluripotent stem cells after a myocardial injury...
September 1, 2017: Circulation Research
https://www.readbyqxmd.com/read/28737465/the-effect-of-ozone-gas-sterilization-on-the-properties-and-cell-compatibility-of-electrospun-polycaprolactone-scaffolds
#20
Carolina Fracalossi Rediguieri, Paul A De Bank, Maria Helena Ambrosio Zanin, Patrícia Leo, Natalia Neto Pereira Cerize, Adriano Marim de Oliveira, Terezinha de Jesus Andreoli Pinto
The growing area of tissue engineering has the potential to alleviate the shortage of tissues and organs for transplantation, and electrospun biomaterial scaffolds are extremely promising devices for translating engineered tissues into a clinical setting. However, to be utilized in this capacity, these medical devices need to be sterile. Traditional methods of sterilization are not always suitable for biomaterials, especially as many commonly used biomedical polymers are sensitive to chemical-, thermal- or radiation-induced damage...
July 30, 2017: Journal of Biomaterials Science. Polymer Edition
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