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Viability assessment transplantation

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https://www.readbyqxmd.com/read/29131417/culturing-with-modified-egm2-medium-enhances-porcine-neonatal-islet-like-cell-clusters-resistance-to-apoptosis-in-islet-xenotransplantation
#1
Xiaoqian Ma, Cejun Yang, Juan Zhang, Jia Wang, Wei Li, Chang Xu, Pengfei Rong, Bin Ye, Minghua Wu, Jianhui Jiang, Shounan Yi, Wei Wang
BACKGROUND: Neonatal pig islet-like cell clusters (NICC) are an attractive source of insulin-producing tissue for potential transplantation treatment of type 1 diabetic patients. However, a considerable loss of NICC after their transplantation due to apoptosis resulted from islet isolation and instant blood-mediated inflammatory reaction remains to be overcome. METHODS: EGM2 medium depleted with hydrocortisone and supplemented with 50 mmol/L isobutylmethylxanthine, 10 mmol/L nicotinamide, and 10 mmol/L glucose was used to culture NICC at day 1, the day after isolation and changed every other day...
November 12, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29129566/pediatric-glioblastoma-cells-inhibit-neurogenesis-and-promote-astrogenesis-phenotypic-transformation-and-migration-of-human-neural-progenitor-cells-within-cocultures
#2
Kurt Farrell, Gautam Mahajan, Parthasarathy Srinivasan, Moo-Yeal Lee, Chandrasekhar R Kothapalli
Neural progenitor cell (NPC) fate is influenced by a variety of biological cues elicited from the surrounding microenvironment and recent studies suggest their possible role in pediatric glioblastoma multiforme (GBM) development. Since a few GBM cells also display NPC characteristics, it is not clear whether NPCs transform to tumor cell phenotype leading to the onset of GBM formation, or NPCs migrate to developing tumor sites in response to paracrine signaling from GBM cells. Elucidating the paracrine interactions between GBM cells and NPCs in vivo is challenging due to the inherent complexity of the CNS...
November 9, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29093531/intraoperative-assessment-of-parathyroid-viability-using-laser-speckle-contrast-imaging
#3
E A Mannoh, G Thomas, C C Solórzano, A Mahadevan-Jansen
Post-surgical hypoparathyroidism and hypocalcemia are known to occur after nearly 50% of all thyroid surgeries as a result of accidental disruption of blood supply to healthy parathyroid glands, which are responsible for regulating calcium. However, there are currently no clinical methods for accurately identifying compromised glands and the surgeon relies on visual assessment alone to determine if any gland(s) should be excised and auto-transplanted. Here, we present Laser Speckle Contrast Imaging (LSCI) for real-time assessment of parathyroid viability...
November 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29065700/murine-bone-marrow-mesenchymal-stromal-cells-respond-efficiently-to-oxidative-stress-despite-the-low-level-of-heme-oxygenases-1-and-2
#4
Witold Norbert Nowak, Hevidar Taha, Neli Kachamakova-Trojanowska, Jacek Stepniewski, Joanna Agata Markiewicz, Anna Kusienicka, Krzysztof Szade, Agata Szade, Karolina Bukowska-Strakova, Karolina Hajduk, Damian Klóska, Aleksandra Kopacz, Anna Grochot-Przeczek, Kathrin Barthenheier, Camille Cauvin, Jozef Dulak, Alicja Jozkowicz
AIMS: Mesenchymal stromal cells (MSCs) are heterogeneous cells from adult tissues, able to differentiate in vitro into adipocytes, osteoblasts or chondrocytes. Such cells are widely studied in regenerative medicine. However, the success of cellular therapy depends on the cell survival. Heme oxygenase-1 (HO-1, encoded by the Hmox1 gene), an enzyme converting heme to biliverdin, carbon monoxide, and Fe2+, is cytoprotective and can affect stem cell performance. Therefore, our study aimed to assess if Hmox1 is critical for survival and functions of murine bone marrow MSCs...
October 25, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/29037941/pre-clinical-assessment-of-the-lovo-device-for-dimethyl-sulfoxide-removal-and-cell-concentration-in-thawed-hematopoietic-progenitor-cell-grafts
#5
Bechara Mfarrej, Guillaume Bouchet, Jérôme Couquiaud, Lionel Regimbaud, Steven Binninger, Mathieu Mercier, Claude Lemarié, Pascal Houzé, Christian Chabannon, Boris Calmels
BACKGROUND: Cryopreserved hematopoietic progenitor cell (HPC) grafts are widely infused to patients with malignant and nonmalignant conditions. Despite reduction of immediate side effects linked to dimethyl sulfoxide (DMSO), cell debris-containing grafts and comparable hematopoietic engraftment between washed and unwashed cryopreserved products, bedside infusion of thawed HPC grafts is still preferred. Introduction of automated devices is important for standardization and consistency of graft manipulation...
October 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29037258/transplantation-of-isl1-cardiac-progenitor-cells-in-small-intestinal-submucosa-improves-infarcted-heart-function
#6
Lingjun Wang, Elizabeth M Meier, Shuo Tian, Ienglam Lei, Liu Liu, Shaoxiang Xian, Mai T Lam, Zhong Wang
BACKGROUND: Application of cardiac stem cells combined with biomaterial scaffold is a promising therapeutic strategy for heart repair after myocardial infarction. However, the optimal cell types and biomaterials remain elusive. METHODS: In this study, we seeded Isl1(+) embryonic cardiac progenitor cells (CPCs) into decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM) to assess the therapeutic potential of Isl1(+) CPCs and the biocompatibility of SIS-ECM with these cells...
October 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29037191/ascorbic-acid-alters-cell-fate-commitment-of-human-neural-progenitors-in-a-wnt-%C3%AE-catenin-ros-signaling-dependent-manner
#7
Tareck Rharass, Margareta Lantow, Adam Gbankoto, Dieter G Weiss, Daniela Panáková, Stéphanie Lucas
BACKGROUND: Improving the neuronal yield from in vitro cultivated neural progenitor cells (NPCs) is an essential challenge in transplantation therapy in neurological disorders. In this regard, Ascorbic acid (AA) is widely used to expand neurogenesis from NPCs in cultures although the mechanisms of its action remain unclear. Neurogenesis from NPCs is regulated by the redox-sensitive WNT/β-catenin signaling pathway. We therefore aimed to investigate how AA interacts with this pathway and potentiates neurogenesis...
October 16, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28990205/developing-a-protocol-for-normothermic-ex-situ-limb-perfusion
#8
Eliana F R Duraes, Maria Madajka, Russell Frautschi, Basem Soliman, Cagri Cakmakoglu, Addison Barnett, Kashyap Tadisina, Qiang Liu, Patrick Grady, Cristiano Quintini, Toshihiro Okamoto, Francis Papay, Antonio Rampazzo, Bahar Bassiri Gharb
BACKGROUND: Ischemia time represents a significant limitation for successful extremity transplantation because of the rapid deterioration of ischemic muscle. Normothermic ex-situ preservation is an emergent method to prolong the organ viability following procurement, by replicating the physiologic conditions. The aim of this study was to develop an ex-situ normothermic limb perfusion system to preserve the viability and function of porcine limbs for 12 hours following procurement. METHODS: A total of 18 swine limbs were perfused...
October 9, 2017: Microsurgery
https://www.readbyqxmd.com/read/28982949/the-existence-of-dead-cells-in-donor-corneal-endothelium-preserved-with-storage-media
#9
Koji Kitazawa, Tsutomu Inatomi, Hidetoshi Tanioka, Satoshi Kawasaki, Hiroko Nakagawa, Osamu Hieda, Hideki Fukuoka, Naoki Okumura, Noriko Koizumi, Bernie Iliakis, Chie Sotozono, Shigeru Kinoshita
AIM: To investigate the viability of donor corneal endothelial cells (CECs) preserved in storage media by histological examination. METHODS: Twenty-eight donor corneas were obtained from SightLife Eye Bank (Seattle, Washington), and redundant peripheral portions of those corneas were used for histological examination after removal of the centre corneal graft for transplantation. To assess cell viability in the corneal endothelium, biostaining experiments were performed using propidium iodide, calcein-AM, Hoechst 33 342, annexin V, anti-vimentin antibody and toluidine blue...
October 5, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28979628/determination-in-vivo-viability-of-a-transfused-platelet-product-by-corrected-count-increment-and-percentage-platelet-response
#10
Nancy Wanjiru Thuku, Kibet Shikuku, Amos Mbugua
INTRODUCTION: For many years, platelet concentrates have been used for the prevention as well as treatment of bleeding disorders, especially in those patients with haematological problems involving platelet disorders as well as refractoriness, In addition, platelet concentrates (PCs) have been widely used to support patients undergoing bone marrow transplantation or who are receiving myelotoxic treatments. The aim of this study was to determine the quality of platelet concentrates by assessing platelet counts, volume, pH changes, swirling, residue of the red blood cells and white blood cell counts...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28977017/real-time-assessment-of-corneal-endothelial-cell-damage-following-graft-preparation-and-donor-insertion-for-dmek
#11
Maninder Bhogal, Chan N Lwin, Xin-Yi Seah, Elavazhagan Murugan, Khadijah Adnan, Shu-Jun Lin, Gary Peh, Jodhbir S Mehta
PURPOSE: To establish a method for assessing graft viability, in-vivo, following corneal transplantation. METHODS: Optimization of calcein AM fluorescence and toxicity assessment was performed in cultured human corneal endothelial cells and ex-vivo corneal tissue. Descemet membrane endothelial keratoplasty grafts were incubated with calcein AM and imaged pre and post preparation, and in-situ after insertion and unfolding in a pig eye model. Global, macroscopic images of the entire graft and individual cell resolution could be attained by altering the magnification of a clinical confocal scanning laser microscope...
2017: PloS One
https://www.readbyqxmd.com/read/28971483/study-of-efficacy-and-safety-of-noncultured-extracted-follicular-outer-root-sheath-cell-suspension-transplantation-in-the-management-of-stable-vitiligo
#12
Pawan Kumar, Neetu Bhari, Manoj K Tembhre, Sujata Mohanty, Sudheer Arava, Vinod K Sharma, Somesh Gupta
BACKGROUND: Noncultured, extracted follicular outer root sheath suspension (NC-EHF-ORS-CS) is a recently introduced technique for the treatment of stable vitiligo. OBJECTIVE: To study the clinical efficacy of this technique and to determine the viability and cell composition of the suspension. METHODS: Twenty-five patients with stable vitiligo were included in this prospective study. Fifty follicles were extracted from occipital scalp and were incubated with trypsin-ethylenediaminetetraacetic acid to separate outer root sheath cells...
October 3, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28962589/multipotent-mesenchymal-stromal-cells-enhance-insulin-secretion-from-human-islets-via-n-cadherin-interaction-and-prolong-function-of-transplanted-encapsulated-islets-in-mice
#13
Elisa Montanari, Raphael P H Meier, Redouan Mahou, Jörg D Seebach, Christine Wandrey, Sandrine Gerber-Lemaire, Leo H Buhler, Carmen Gonelle-Gispert
BACKGROUND: Multipotent mesenchymal stromal cells (MSC) enhance viability and function of islets of Langerhans. We aimed to examine the interactions between human MSC and human islets of Langerhans that influence the function of islets. METHODS: Human MSC and human islets (or pseudoislets, obtained after digestion and reaggregation of islet cells) were cocultured with or without cellular contact and glucose-stimulated insulin secretion assays were performed to assess cell function...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28958037/evaluation-of-changes-in-cartilage-viability-in-detergent-treated-tracheal-grafts-for-immunosuppressant-free-allotransplantation-in-dogs
#14
Tao Lu, Yiwei Huang, Yulei Qiao, Yongxing Zhang, Yu Liu
OBJECTIVES: The first tissue-engineered clinical tracheal transplant prepared using the detergent-enzymatic method resulted in graft stenosis, possibly from detergent-enzymatic method-induced graft non-viability. We reported on the transplantation of de-epithelialized tracheal allografts while maintaining cartilage viability in dogs. No lethal stenosis occurred in allografts. Herein, on the basis of previous experimentation, we assessed cartilage viability in detergent-treated cartilages...
September 6, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28939367/comparison-of-three-human-platelet-lysates-used-as-supplements-for-in-vitro-expansion-of-corneal-endothelium-cells
#15
Tsung-Jen Wang, Ming-Sheng Chen, Ming-Li Chou, Hsiu-Chen Lin, Jerard Seghatchian, Thierry Burnouf
BACKGROUND: Human platelet lysates (HPLs) are emerging as the new gold standard supplement of growth media for ex vivo expansion of cells for transplant. However, variations do exist in the way how HPLs are prepared. In particular, uncertainties still exist regarding the type of HPL most suitable for corneal endothelium cells (CEC) expansion, especially as these cells have limited proliferative capacity. MATERIAL AND METHODS: Three distinct HPL preparations were produced, with or without calcium chloride/glass beads activation, and with or without heat treatment at 56°C for 30min...
August 31, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28936404/-18-f-fdg-pet-for-assessing-biological-viability-and-prognosis-in-liver-transplant-patients-with-hepatocellular-carcinoma
#16
REVIEW
Arno Kornberg, Martina Schernhammer, Helmut Friess
Liver transplantation (LT) has become standard of care in patients with non-resectable early stage hepatocellular carcinoma (HCC) in liver cirrhosis. Currently, patient selection for LT is strictly based on tumor size and number, provided by the Milan criteria. This may, however, exclude patients with advanced tumor load but favourable biology from a possibly curative treatment option. It became clear in recent years that biological tumor viability rather than tumor macromorphology determines posttransplant outcome...
September 28, 2017: Journal of Clinical and Translational Hepatology
https://www.readbyqxmd.com/read/28914392/alginate-hyaluronic-acid-hydrogel-delivery-system-characteristics-regulate-the-differentiation-of-periodontal-ligament-stem-cells-toward-chondrogenic-lineage
#17
Sahar Ansari, Ivana M Diniz, Chider Chen, Tara Aghaloo, Benjamin M Wu, Songtao Shi, Alireza Moshaverinia
Cartilage tissue regeneration often presents a challenging clinical situation. Recently, it has been shown that Periodontal Ligament Stem Cells (PDLSCs) possess high chondrogenic differentiation capacity. In this study, we developed a stem cell delivery system based on alginate/hyaluronic acid (HA) loaded with TGF-β1 ligand, encapsulating PDLSCs; and investigated the chondrogenic differentiation of encapsulated cells in alginate/HA hydrogel microspheres in vitro and in vivo. The results showed that PDLSCs, as well as human bone marrow mesenchymal stem cells (hBMMSCs), as the positive control, were stained positive for both toluidine blue and alcian blue staining, while exhibiting high levels of gene expression related to chondrogenesis (Col II, Aggrecan and Sox-9), as assessed via qPCR...
September 15, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28901972/normothermic-machine-perfusion-of-the-kidney
#18
Annemarie Weissenbacher, James Hunter
PURPOSE OF REVIEW: Normothermic machine perfusion (NMP) is a preservation method that is generating increasing interest. The aim of this review is to summarise the current status of NMP in regards of kidney viability assessment, reducing organ damage and improving transplant logistics. RECENT FINDINGS: The results of recent large animal experiments and clinical trials show that continuous prolonged normothermic ex-vivo kidney perfusion appears better than a brief period of NMP after static cold storage in terms of renal injury and function...
December 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/28889066/effect-of-lentivirus-mediated-gene-silencing-targeting-toll-like-receptor-2-on-corneal-allograft-transplantation-in-rats
#19
Lang Bai, Weiyi Liang, Minting Chen, Yacouba Cissé, Jing Liu, Yaru Su, Jian Yu, Qiong Liu
AIM: The present work aims to assess the effectiveness of lentiviral vector (LV) mediated Toll-like receptor 2 (TLR2) gene silencing in the survival of transplanted corneal allografts, against immune rejection, in rats. METHODS: LV mediated TLR2 small interference RNA (SiRNA) with enhanced green fluorescent protein (eGFP) [LV-TLR2-siRNA-eGFP] was realised and transfected to both rat corneal epithelial (EC) and stromal cells (SC). Multiplicity of infection (MOI) was optimized for transfection efficiency using flow cytometric (FCM) analysis...
November 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28887978/neuro-differentiated-ntera2-cancer-stem-cells-encapsulated-in-alginate-beads-first-evidence-of-biological-functionality
#20
Ilaria Cacciotti, Claudia Ceci, Alessandra Bianco, Giuseppa Pistritto
The present communication investigates an application of alginate encapsulation technology to the differentiation of the embryonic cancer stem NTera2 cells (NT2) into dopamine-producing cells. The encapsulation of cells in polymeric beads allows their immune isolation and makes them eligible for transplantation, thus representing a promising biotech tool for the delivery of biologically active compounds to the brain. The polysaccharide alginate is one of the most commonly used material for this procedure since it is well tolerated by various tissues, including the brain...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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