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Viability assessment transplantation

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https://www.readbyqxmd.com/read/29781952/optimizing-temperature-and-oxygen-supports-long-term-culture-of-human-islets
#1
Hirotake Komatsu, Jeffrey Rawson, Leonard Medrano, Colin A Cook, Alyssa Barriga, Nelson Gonzalez, Mayra Salgado, Keiko Omori, Fouad Kandeel, Yu-Chong Tai, Yoko Mullen
BACKGROUND: Islet transplantation is a promising treatment for type-1 diabetes; however, donor shortage is a concern. Even when a pancreas is available, low islet yield limits the success of transplantation. Islet culture enables pooling of multiple low-yield isolations into an effective islet mass, but isolated islets rapidly deteriorate under conventional culture conditions. Oxygen depletion in the islet core, which leads to central necrosis and volume loss, is one of the major reasons for this deterioration...
May 16, 2018: Transplantation
https://www.readbyqxmd.com/read/29768256/overexpression-of-pck1-gene-antagonizes-hepatocellular-carcinoma-through-the-activation-of-gluconeogenesis-and-suppression-of-glycolysis-pathways
#2
Yufu Tang, Yibing Zhang, Chunhui Wang, Zhongyi Sun, Longfei Li, Shuqun Cheng, Wenping Zhou
BACKGROUND/AIMS: Gluconeogenesis, a reverse process of glycolysis, is suppressed in neoplastic livers. Cytoplasmic phosphoenolpyruvate carboxykinase (PEPCK-C/PCK1, encoded by PCK1) is a step limiting enzyme of gluconeogenesis. The induced expression of the factor is reported to initiate gluconeogenesis process and antagonize hepatocellular carcinoma (HCC). In the current study, the effect of the modulation of PCK1 expression on HCC was assessed. METHODS: The levels of PCK1 in clinical HCC tissues and different HCC cell lines were investigated with real time quantitative PCR, immunochemistry, and western blotting...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29761823/erythropoietin-promotes-network-formation-of-transplanted-adipose-tissue-derived-microvascular-fragments
#3
P Karschnia, C Scheuer, A Heß, T Später, M D Menger, M W Laschke
The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls...
May 9, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29758129/twenty-four-hour-normothermic-perfusion-of-discarded-human-kidneys-with-urine-recirculation
#4
Annemarie Weissenbacher, Letizia Lo Faro, Olga Boubriak, Maria F Soares, Ian S Roberts, James P Hunter, Daniel Voyce, Nikolay Mikov, Andrew Cook, Rutger J Ploeg, Constantin C Coussios, Peter J Friend
Transportable normothermic kidney perfusion for 24 hours or longer could enable viability assessment of marginal grafts, increased organ utilization and improved transplantation logistics. Eleven clinically-declined kidneys were perfused normothermically, six from donors after brain stem death (DBD, median cold ischemia time 33±36.9 hours) and five from donors after circulatory death (DCD, 36.2±38.3 hours): n=3 kidneys were perfused using Ringer's lactate to replace excreted urine volume, and n=8 using urine recirculation to maintain perfusate volume without fluid replenishment...
May 14, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29758019/ginkgo-biloba-l-extract-reduces-h2o2-induced-bone-marrow-mesenchymal-stem-cells-cytotoxicity-by-regulating-mitogen-activated-protein-kinase-mapk-signaling-pathways-and-oxidative-stress
#5
Ao Wang, Qiwei Yang, Qiuju Li, Xiaonan Wang, Shuhong Hao, Jincheng Wang, Ming Ren
BACKGROUND The oxidative stress environment of pathological tissue has an adverse effect on the survival of bone marrow mesenchymal stem cells (BMSCs) transplantation. Ginkgo biloba L. extract (EGB) has a potent antioxidant effect. In this research, we assessed the protective effects of EGB and EGB-Containing Serum (EGB CS) on BMSCs against injury induced by hydrogen peroxide (H2O2). MATERIAL AND METHODS BMSCs were pretreated with EGB or EGB CS and treated with H2O2. The cell counting kit-8 (CCK-8) method was utilized to detect cell viability...
May 14, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29736937/pericardial-application-as-a-new-route-for-implanting-stem-cell-cardiospheres-to-treat-myocardial-infarction
#6
Jianhua Zhang, Zheng Wu, Zepei Fan, Zixi Qin, Yingwei Wang, Jiayuan Chen, Maoxiong Wu, Yangxin Chen, Changhao Wu, Jingfeng Wang
KEY POINTS: Cardiospheres (CSps) are a promising new form of cardiac stem cells with advantage over other stem cells for myocardial regeneration, but direct implantation of CSps by conventional routes has been limited due to potential embolism. We have implanted CSps into the pericardial cavity and systematically demonstrated its efficacy regarding myocardial infarction. Stem cell potency and cell viability can be optimized in vitro prior to implantation by pre-conditioning CSps with pericardial fluid and hydrogel packing...
May 7, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29733421/chemotherapeutic-drugs-alter-functional-properties-and-proteome-of-mouse-testicular-germ-cells-in-vitro
#7
Polash Chandra Karmakar, Yeon-Jin Cho, Yong-Hee Kim, Sang-Eun Jung, Ju-Hee Jin, Bang-Jin Kim, Woo-Sung Kwon, Young-Hyun Kim, Myung-Geol Pang, Buom-Yong Ryu
Many of the testicular cancer-survived patients, treated with chemotherapeutic drugs, show infertility, preimplantation and postimplantation loss, and germ cell abnormality. Studies examining the negative effects of chemotherapeutic drugs on testicular germ cells are ongoing; however, information on the stemness properties and proteomic profiles of these cells are lacking. The present study investigated the effects of chemotherapeutic drugs etoposide, cisplatin, bleomycin, and their combination (BEP) on the physiology and stem cell activity of mouse germ cells in vitro...
April 27, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29729330/paraoxonase-2-prevents-the-development-of-heart-failure
#8
Wei Li, David Kennedy, Zhili Shao, Xi Wang, Andre Klaassen Kamdar, Malory Weber, Kayla Mislick, Kathryn Kiefer, Rommel Morales, Brendan Agatisa-Boyle, Diana M Shih, Srinivasa T Reddy, Christine S Moravec, W H Wilson Tang
BACKGROUND: Mitochondrial oxidation is a major source of reactive oxygen species (ROS) and mitochondrial dysfunction plays a central role in development of heart failure (HF). Paraoxonase 2 deficient (PON2-def) mitochondria are impaired in function. In this study, we tested whether PON2-def aggravates HF progression. METHODS AND RESULTS: Using qPCR, immunoblotting and lactonase activity assay, we demonstrate that PON2 activity was significantly decreased in failing hearts despite increased PON2 expression...
May 2, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29716634/successful-fertility-following-optimized-perfusion-and-cryopreservation-of-whole-ovary-and-allotransplantation-in-a-premature-ovarian-insufficiency-rat-model
#9
Yan Ding, Jia-Liang Shao, Jun-Wei Li, Ying Zhang, Kai-Hua Hong, Ke-Qin Hua, Xiang Wang
BACKGROUND: Fertility preservation by whole ovary cryopreservation and transplantation (WOCP&TP) with vascular anastomosis requires successful cryopreservation. In this study, we investigated the possibility of restoring ovarian function and natural fertility after WOCP&TP in a premature ovarian insufficiency (POI) rat model. The influence of cryopreservation on the offspring of rats following WOCP&TP was also explored. METHOD: Rats aged 8-10 weeks were used as donors and recipients for allotransplantation...
May 2, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/29705807/rapamycin-protects-skin-fibroblasts-from-ultraviolet-b-induced-photoaging-by-suppressing-the-production-of-reactive-oxygen-species
#10
Dengke Qin, Runjian Ren, Chuanlong Jia, Yongzhou Lu, Qingjian Yang, Liang Chen, Xinyuan Wu, Jingjing Zhu, Yu Guo, Ping Yang, Yiqun Zhou, Ningwen Zhu, Bo Bi, Tianyi Liu
BACKGROUND/AIMS: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, thereby inducing skin photoaging. Murine dermal fibroblasts (MDFs) were subjected to a series of 4 sub-cytotoxic UVB doses (120 mJ/cm2), resulting in changes in cell shape, DNA damage, cell cycle arrest, extracellular matrix variations, reactive oxygen species (ROS) generation, and alterations in major intracellular antioxidant and cellular autophagy levels. Rapamycin (RAPA) is a new macrolide immunosuppressive agent that is primarily used in oncology, cardiology, and transplantation medicine and has been found to extend the lifespan of genetically heterogeneous mice...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29702010/pulp-regeneration-by-3-dimensional-dental-pulp-stem-cell-constructs
#11
Y Itoh, J I Sasaki, M Hashimoto, C Katata, M Hayashi, S Imazato
Dental pulp regeneration therapy for the pulpless tooth has attracted recent attention, and clinical trial studies are underway with the tissue engineering approach. However, there remain many concerns, including the extended period for regenerating the dental pulp. In addition, the use of scaffolds increases the risk of inflammation and infection. To establish a basic technology for novel dental pulp regenerative therapy that allows transplant of pulp-like tissue, we attempted to fabricate scaffold-free 3-dimensional (3D) cell constructs composed of dental pulp stem cells (DPSCs)...
April 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29700404/preservation-of-rat-limbs-by-hyperbaric-carbon-monoxide-and-oxygen
#12
Naoyuki Hatayama, Shuichi Hirai, Munekazu Naito, Hayato Terayama, Jun Araki, Hiroki Yokota, Masayuki Matsushita, Xiao-Kang Li, Masahiro Itoh
Cold ischemia times ranging from <6 h to as long as 24 h are generally quoted as the limits for attempting the replantation of amputated extremities. In this study, we aimed to assess the effect of hyperbaric carbon monoxide (CO) and oxygen (O2 ) on rat limb preservation. Donor rat limbs were preserved in a chamber filled with hyperbaric CO and O2 for 3 days (CO + O2 3 days) or 7 days (CO + O2 7 days). Positive and negative control groups were created by using non-preserved limbs (NP) and limbs wrapped in saline-moistened gauze for 3 days (SMG 3 days), respectively...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29697462/ex-vivo-machine-perfusion-for-kidney-preservation
#13
Matyas Hamar, Markus Selzner
PURPOSE OF REVIEW: Machine perfusion is a novel strategy to decrease preservation injury, improve graft assessment, and increase organ acceptance for transplantation. This review summarizes the current advances in ex-vivo machine-based kidney preservation technologies over the last year. RECENT FINDINGS: Ex-vivo perfusion technologies, such as hypothermic and normothermic machine perfusion and controlled oxygenated rewarming, have gained high interest in the field of organ preservation...
April 24, 2018: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/29693963/wistar-rats-immature-testicular-tissue-vitrification-and-heterotopic-grafting
#14
Larissa Benvenutti, Rafael Alonso Salvador, David Til, Alfred Paul Senn, David Rivero Tames, Nicole Louise Lângaro Amaral, Vera Lúcia Lângaro Amaral
OBJECTIVE: To evaluate the efficiency of two vitrification protocols for rat immature testicular tissue and heterotopic transplantation. METHODS: Twenty-four pre-pubertal Wistar rats were divided into three groups (n=8). After orchiectomy, testicular fragments (3mm) from Groups 1 and 2 were vitrified with different cryoprotectant concentration solutions, using sterile inoculation loops as support. After warming up, the fragments were submitted to cell viability assessment by Trypan blue and histological evaluation...
April 25, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29689547/sirt3-facilitates-amniotic-fluid-stem-cells-to-repair-diabetic-nephropathy-through-protecting-mitochondrial-homeostasis-by-modulation-of-mitophagy
#15
Jianxun Feng, Chang Lu, Qin Dai, Junqin Sheng, Min Xu
BACKGROUND/AIMS: Amniotic fluid stem cells (AFSCs) transplantation is a promising therapeutic strategy for diabetic nephropathy. Sirtuin3 (SIRT3) is a novel mitochondrial protective factor. In the present study, we aimed to investigate whether SIRT3 protects against hyperglycemia-induced AFSCs damage and enhances the therapeutic efficiency of AFSCs in diabetic nephropathy. METHODS: To establish the diabetic nephropathy model, db/ db mice were used. AFSCs were obtained and transplanted into the kidney tissue of db/ db mice...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29673400/long-noncoding-rna-h19-upregulates-vascular-endothelial-growth-factor-a-to-enhance-mesenchymal-stem-cells-survival-and-angiogenic-capacity-by-inhibiting-mir-199a-5p
#16
Jingying Hou, Lingyun Wang, Quanhua Wu, Guanghui Zheng, Huibao Long, Hao Wu, Changqing Zhou, Tianzhu Guo, Tingting Zhong, Lei Wang, Xuxiang Chen, Tong Wang
BACKGROUND: Currently, the overall therapeutic efficiency of mesenchymal stem cells (MSCs) transplantation for the treatment of cardiovascular disease is not satisfactory. The low viability and angiogenic capacity of the implanted cells in the local infarct tissues restrict their further application. Evidence shows that long noncoding RNA H19 (lncRNA-H19) mediates cell survival and angiogenesis. Additionally, it is also involved in MSCs biological activities. This study aimed to explore the functional role of lncRNA-H19 in MSCs survival and angiogenic capacity as well as the underlying mechanism...
April 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29671177/tri-modal-in-vivo-imaging-of-pancreatic-islets-transplanted-subcutaneously-in-mice
#17
Sayuan Liang, Karim Louchami, Bryan Holvoet, Rein Verbeke, Christophe M Deroose, Bella Manshian, Stefaan J Soenen, Ine Lentacker, Uwe Himmelreich
PURPOSE: Transplantation of pancreatic islets (PIs) is a promising therapeutic approach for type 1 diabetes. The main obstacle for this strategy is that the outcome of islet engraftment depends on the engraftment site. It was our aim to develop a strategy for using non-invasive imaging techniques to assess the location and fate of transplanted PIs longitudinally in vivo. PROCEDURES: In order to overcome the limitations of individual imaging techniques and cross-validate findings by different modalities, we have combined fluorine magnetic resonance imaging (F-19 MRI), fluorescence imaging (FLI), and bioluminescent imaging (BLI) for studying subcutaneously transplanted PIs and beta cell-like cells (INS-1E cell line) in vivo...
April 18, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/29670285/a-randomized-trial-of-normothermic-preservation-in-liver-transplantation
#18
David Nasralla, Constantin C Coussios, Hynek Mergental, M Zeeshan Akhtar, Andrew J Butler, Carlo D L Ceresa, Virginia Chiocchia, Susan J Dutton, Juan Carlos García-Valdecasas, Nigel Heaton, Charles Imber, Wayel Jassem, Ina Jochmans, John Karani, Simon R Knight, Peri Kocabayoglu, Massimo Malagò, Darius Mirza, Peter J Morris, Arvind Pallan, Andreas Paul, Mihai Pavel, M Thamara P R Perera, Jacques Pirenne, Reena Ravikumar, Leslie Russell, Sara Upponi, Chris J E Watson, Annemarie Weissenbacher, Rutger J Ploeg, Peter J Friend
Liver transplantation is a highly successful treatment, but is severely limited by the shortage in donor organs. However, many potential donor organs cannot be used; this is because sub-optimal livers do not tolerate conventional cold storage and there is no reliable way to assess organ viability preoperatively. Normothermic machine perfusion maintains the liver in a physiological state, avoids cooling and allows recovery and functional testing. Here we show that, in a randomized trial with 220 liver transplantations, compared to conventional static cold storage, normothermic preservation is associated with a 50% lower level of graft injury, measured by hepatocellular enzyme release, despite a 50% lower rate of organ discard and a 54% longer mean preservation time...
April 18, 2018: Nature
https://www.readbyqxmd.com/read/29665002/three-dimensional-collagenous-niche-and-azacytidine-selectively-promote-time-dependent-cardiomyogenesis-from-human-bone-marrow-derived-msc-spheroids
#19
Jyotsna Joshi, Gautam Mahajan, Chandrasekhar R Kothapalli
Endogenous adult cardiac regenerative machinery is not capable of replacing the lost cells following myocardial infarction, often leading to permanent alterations in structure-function-mechanical properties. Regenerative therapies based on delivering autologous stem cells within an appropriate 3D milieu could meet such demand, by enabling homing and directed differentiation of the transplanted cells into lost specialized cell populations. Since type I collagen is the predominant cardiac tissue matrix protein, we here optimized the 3D niche which could promote time-dependent evolution of cardiomyogenesis from human bone marrow-derived mesenchymal stem cells (BM-MSC)...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29661435/transient-elastography-in-acute-cellular-rejection-following-liver-transplantation-systematic-review
#20
L S Nacif, C D C Gomes, M N Mischiatti, V Kim, D Paranaguá-Vezozzo, G L Reinoso, F J Carrilho, L C D'Albuquerque
BACKGROUND: Transient elastography (TE) is a noninvasive technique that measures liver stiffness. When an inflammatory process is present, this is shown by elevated levels of stiffness. Acute cellular rejection (ACR) is a consequence of an inflammatory response directed at endothelial and bile epithelial cells, and it is diagnosed through liver biopsy. This is a systematic review of the viability of TE in ACR following liver transplantation. METHODS: The Cochrane Library, Embase, and Medline PubMed databases were searched and updated to November 2016...
April 2018: Transplantation Proceedings
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