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https://www.readbyqxmd.com/read/27885560/expression-of-human-endogenous-retrovirus-k-and-w-in-babies
#1
L H S Nali, A C S Oliveira, D O Alves, G S Caleiro, C F Nunes, D Gerhardt, R C M Succi, Camila M Romano, D M Machado
Here we determined the relative expression of HERV-K and W proviruses in HIV infected and non-infected mothers as well as their respective babies up to 1 year-old. HIV-infected mothers, their babies and uninfected control groups presented expression of both HERV-K and HERV-W with relatively high frequency. While the level of HERV-K expression was similar among groups, the level of HERV-W expression in HIV-infected mothers was four-fold higher than the uninfected mothers from the control group (p < 0.01)...
November 24, 2016: Archives of Virology
https://www.readbyqxmd.com/read/27784826/the-harsh-life-of-an-urban-tree-the-effect-of-a-single-pulse-of-ozone-in-salt-stressed-quercus-ilex-saplings
#2
Lucia Guidi, Damiano Remorini, Lorenzo Cotrozzi, Tommaso Giordani, Giacomo Lorenzini, Rossano Massai, Cristina Nali, Lucia Natali, Elisa Pellegrini, Alice Trivellini, Alberto Vangelisti, Paolo Vernieri, Marco Landi
Ozone (O3) and salinity are usually tested as combined factors on plant performance. However, the response to a single episode of O3 in plants already stressed by an excess of NaCl as occurs in the natural environment has never been investigated, but is important given that it is commonly experienced in Mediterranean areas. Three-year-old Quercus ilex L. (holm oak) saplings were exposed to salinity (150 mM NaCl, 15 days), and the effect on photosynthesis, hydric relations and ion partitioning was evaluated (Experiment I)...
October 26, 2016: Tree Physiology
https://www.readbyqxmd.com/read/27777581/trichoderma-harzianum-t-22-induces-systemic-resistance-in-tomato-infected-by-cucumber-mosaic-virus
#3
Antonella Vitti, Elisa Pellegrini, Cristina Nali, Stella Lovelli, Adriano Sofo, Maria Valerio, Antonio Scopa, Maria Nuzzaci
Understanding the induction of plant defenses against viruses using biocontrol agents is essential for developing new strategies against these pathogens, given the ineffectiveness of chemical treatments. The ability of Trichoderma harzianum, strain T-22 (T22) to control Cucumber mosaic virus (CMV) in Solanum lycopersicum var. cerasiforme plants and the changes in the physiology of tomato treated/infected with T22/CMV were examined. Plant growth-promoting effects, photosynthetic performance, reactive oxygen species scavenging enzymes, and phytohormones were investigated...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27513910/polyandry-predation-and-the-evolution-of-frog-reproductive-modes
#4
Kelly R Zamudio, Rayna C Bell, Renato C Nali, Célio F B Haddad, Cynthia P A Prado
Frog reproductive modes are complex phenotypes that include egg/clutch characteristics, oviposition site, larval development, and sometimes, parental care. Two evident patterns in the evolution of these traits are the higher diversity of reproductive modes in the tropics and the apparent progression from aquatic to terrestrial reproduction, often attributed to higher fitness resulting from decreased predation on terrestrial eggs and tadpoles. Here, we propose that sexual selection-and not only natural selection due to predation-favors terrestrial breeding by reducing the loss of fitness due to polyandry...
September 2016: American Naturalist
https://www.readbyqxmd.com/read/27464945/polyomavirus-detection-in-multiple-sclerosis-patients-under-natalizumab-therapy-profile-and-frequency-of-urinary-shedding
#5
Lhs Nali, McD Fink, G S do Olival, L Moraes, D Callegaro, C P Tilbery, Jeb Vidal, L M Sumita, A C Penalva-de-Oliveira, C M Romano
Patients undergoing Natalizumab therapy are at risk of Progressive Multifocal Leukoencephalopathy. Besides JCV, BK polyomavirus might represent an additional concern for such patients since it can also infect CNS cells. Currently, data regarding the presence of anti-JCV antibodies added to previous immunosuppressive therapy and prolonged NTZ therapy has been used to classify patients at risk of developing PML. Here we investigated the profile shedding of JCV and BKV in MS patients during treatment with Natalizumab...
July 28, 2016: Journal of Medical Virology
https://www.readbyqxmd.com/read/27434673/nali-h1-a-universal-synthetic-library-of-humanized-nanobodies-providing-highly-functional-antibodies-and-intrabodies
#6
Sandrine Moutel, Nicolas Bery, Virginie Bernard, Laura Keller, Emilie Lemesre, Ario de Marco, Laetitia Ligat, Jean-Christophe Rain, Gilles Favre, Aurélien Olichon, Franck Perez
In vitro selection of antibodies allows to obtain highly functional binders, rapidly and at lower cost. Here, we describe the first fully synthetic phage display library of humanized llama single domain antibody (NaLi-H1: Nanobody Library Humanized 1). Based on a humanized synthetic single domain antibody (hs2dAb) scaffold optimized for intracellular stability, the highly diverse library provides high affinity binders without animal immunization. NaLi-H1 was screened following several selection schemes against various targets (Fluorescent proteins, actin, tubulin, p53, HP1)...
2016: ELife
https://www.readbyqxmd.com/read/27327837/coordination-reactions-of-2-pyridinecarboxaldehyde-phenylhydrazonatolithium-with-selected-transition-metal-zn-sn-fe-co-ni-and-zr-chlorides-and-its-coupling-reaction-with-dichloromethane
#7
Xin-E Duan, Hong-Bo Tong, Xue-Hong Wei, He-Ping Shi, Sheng-Di Bai, Tao Bai, Jing Zhang, Yong-Bin Zhang, Dian-Sheng Liu
The reactions of 2-pyridinecarboxaldehyde-phenylhydrazonatolithium C5H4Npy-CH[double bond, length as m-dash]Ni-NaLi(Ph) (abbreviated as Li) with a 1/2 equivalent of anhydrous metal (Zn, Sn, Fe and Co) chlorides or NiCl2(DME) (DME = 1, 2-dimethoxyethane) produced the corresponding mononuclear metal(ii) complexes ( and ), in which each ligand acts as a bidentate ligand and the coordination geometries around the metals are shown to be tetrahedral within the complexes , , and , respectively, and a tetragonal pyramid in the complex ...
July 5, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27313581/suppression-substractive-hybridization-and-ngs-reveal-differential-transcriptome-expression-profiles-in-wayfaring-tree-viburnum-lantana-l-treated-with-ozone
#8
Elena Gottardini, Antonella Cristofori, Elisa Pellegrini, Nicola La Porta, Cristina Nali, Paolo Baldi, Gaurav Sablok
Tropospheric ozone (O3) is a global air pollutant that causes high economic damages by decreasing plant productivity. It enters the leaves through the stomata, generates reactive oxygen species, which subsequent decrease in photosynthesis, plant growth, and biomass accumulation. In order to identify genes that are important for conferring O3 tolerance or sensitivity to plants, a suppression subtractive hybridization analysis was performed on the very sensitive woody shrub, Viburnum lantana, exposed to chronic O3 treatment (60 ppb, 5 h d(-1) for 45 consecutive days)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27272053/lamp5-fine-tunes-gabaergic-synaptic-transmission-in-defined-circuits-of-the-mouse-brain
#9
Marie-Catherine Tiveron, Corinne Beurrier, Claire Céni, Naly Andriambao, Alexis Combes, Muriel Koehl, Nicolas Maurice, Evelina Gatti, Dhoher Nora Abrous, Lydia Kerkerian-Le Goff, Philippe Pierre, Harold Cremer
LAMP5 is member of the LAMP family of membrane proteins. In contrast to the canonical members of this protein family, LAMP1 and LAMP2, which show widespread expression in many tissues, LAMP 5 is brain specific in mice. In C. elegans, the LAMP5 ortholog UNC-46 has been suggested to act a trafficking chaperone, essential for the correct targeting of the nematode vesicular GABA-transporter UNC-47. We show here that in the mouse brain LAMP5 is expressed in subpopulations of GABAergic forebrain neurons in the striato-nigral system and the olfactory bulb...
2016: PloS One
https://www.readbyqxmd.com/read/27187463/correlation-between-nutrition-and-symptoms-nutritional-survey-of-children-with-autism-spectrum-disorder-in-chongqing-china
#10
Xiao Liu, Juan Liu, Xueqin Xiong, Ting Yang, Nali Hou, Xiaohua Liang, Jie Chen, Qian Cheng, Tingyu Li
Restricted diets and inadequate nutrient intake of children with autism spectrum disorder (ASD) have been reported. This study examined the nutritional statuses of children with ASD and the relationships between their behaviors and nutritional intake. A total of 154 children with ASD (age = 5.21 ± 1.83 years) and 73 typically-developing (TD) children (age = 4.83 ± 0.84 years) from Chongqing, China, were enrolled. The severity of ASD was evaluated using the Childhood Autism Rating Scale (CARS). The serum ferritin, folate, vitamin B12, 25(OH) vitamin D, and vitamin A concentrations in the children with ASD were determined...
2016: Nutrients
https://www.readbyqxmd.com/read/27178161/potential-measles-transmission-risk-in-mass-gatherings-are-we-safe-for-the-olympic-games-rio-2016
#11
Luiz Henrique da Silva Nali, Dennis Minoru Fujita, Felipe Scassi Salvador, Maria Cristina Domingues da Silva Fink, Heitor Franco de Andrade, Cláudio Sérgio Pannuti, Expedito José de Albuquerque Luna
No abstract text is available yet for this article.
April 2016: Journal of Travel Medicine
https://www.readbyqxmd.com/read/27101526/pre-transplant-shedding-of-bk-virus-in-urine-is-unrelated-to-post-transplant-viruria-and-viremia-in-kidney-transplant-recipients
#12
C S Bicalho, R R Oliveira, L C Pierrotti, M C D S Fink, P R P Urbano, L H S Nali, E J A Luna, C M Romano, D R David, E David-Neto, C S Pannuti
BK virus-(BKV) associated nephropathy (BKVN) is a major cause of allograft injury in kidney transplant recipients. In such patients, subclinical reactivation of latent BKV infection can occur in the pre-transplant period. The purpose of this study was to determine whether urinary BKV shedding in the immediate pre-transplant period is associated with a higher incidence of viruria and viremia during the first year after kidney transplantation. We examined urine samples from 34 kidney transplant recipients, using real-time quantitative polymerase chain reaction to detect BKV...
July 2016: Clinical Transplantation
https://www.readbyqxmd.com/read/27027366/sex-differences-in-high-sensitivity-c-reactive-protein-in-subjects-with-risk-factors-of-metabolic-syndrome
#13
Vinicius Pacheco Garcia, Helena Naly Miguens Rocha, Allan Robson Kluser Sales, Natália Galito Rocha, Antonio Claudio Lucas da Nóbrega
BACKGROUND: Metabolic syndrome (MetS) is associated with a higher risk of all-cause mortality. High-sensitivity C-reactive protein (hsCRP) is a prototypic marker of inflammation usually increased in MetS. Women with MetS-related diseases present higher hsCRP levels than men with MetS-related diseases, suggesting sex differences in inflammatory markers. However, it is unclear whether serum hsCRP levels are already increased in men and/or women with MetS risk factors and without overt diseases or under pharmacological treatment...
March 2016: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/26959414/mir-3162-3p-is-a-novel-microrna-that-exacerbates-asthma-by-regulating-%C3%AE-catenin
#14
Chao Fang, Weihong Lu, Chengyan Li, Xi Peng, Yang Wang, Xiulan Huang, Zhihong Yao, Nali Cai, Yuge Huang, Xingliang Zhang, Jianxin Tan
Asthma is a common chronic respiratory disease. In a previous study, we found several circulating microRNA signatures associated with childhood asthma and selected miR-3162-3p for subsequent studies. Since the target proteins and underlying molecular mechanisms of miR-3162-3p in asthma etiopathogenesis are not well characterized, we designed this study to clarify its role. We employed bioinformatics and quantitative PCR methods as a first step to determine the target of miR-3162-3p, and we elucidated β-catenin...
2016: PloS One
https://www.readbyqxmd.com/read/26918569/salt-tolerant-rootstock-increases-yield-of-pepper-under-salinity-through-maintenance-of-photosynthetic-performance-and-sinks-strength
#15
Consuelo Penella, Marco Landi, Lucia Guidi, Sergio G Nebauer, Elisa Pellegrini, Alberto San Bautista, Damiano Remorini, Cristina Nali, Salvador López-Galarza, Angeles Calatayud
The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a rootstock was assessed in two experiments. In a first field experiment under natural salinity conditions, we observed a larger amount of marketable fruit (+75%) and lower Blossom-end Root incidence (-31%) in commercial pepper cultivar Adige (A) grafted onto A25 (A/A25) when compared with ungrafted plants. In order to understand this behavior a second greenhouse experiment was conducted to determine growth, mineral partitioning, gas exchange and chlorophyll a fluorescence parameters, antioxidant systems and proline content in A and A/A25 plants under salinity conditions (80 mM NaCl for 14 days)...
April 1, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/26866739/prevalence-of-idiopathic-scoliosis-in-chinese-schoolchildren-a-large-population-based-study
#16
Fan Hengwei, Huang Zifang, Wang Qifei, Tan Weiqing, Deng Nali, Yu Ping, Yang Junlin
STUDY DESIGN: This is a population-based, cross-sectional study of school scoliosis screening program in mainland China. OBJECTIVE: The aim of this study was to assess current prevalence and distribution of idiopathic scoliosis (IS) in schoolchildren and to compare with the results of previous studies. SUMMARY OF BACKGROUND DATA: The feasibility and predictive values of scoliosis screening programs for school-age children remains controversial and many of these programs have recently been discontinued in different countries...
February 2016: Spine
https://www.readbyqxmd.com/read/26766745/endogenous-il-6-of-mesenchymal-stem-cell-improves-behavioral-outcome-of-hypoxic-ischemic-brain-damage-neonatal-rats-by-supressing-apoptosis-in-astrocyte
#17
Yan Gu, Mulan He, Xiaoqin Zhou, Jinngjing Liu, Nali Hou, Tan Bin, Yun Zhang, Tingyu Li, Jie Chen
Mesenchymal stem cell (MSC) transplantation reduces the neurological impairment caused by hypoxic-ischemic brain damage (HIBD) via immunomodulation. In the current study, we found that MSC transplantation improved learning and memory function and enhanced long-term potentiation in neonatal rats subjected to HIBD and the amount of IL-6 released from MSCs was far greater than that of other cytokines. However, the neuroprotective effect of MSCs infected with siIL-6-transduced recombinant lentivirus (siIL-6 MSCs) was significantly weakened in the behavioural tests and electrophysiological analysis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26733148/characterization-and-relative-quantification-of-phospholipids-based-on-methylation-and-stable-isotopic-labeling
#18
Tanxi Cai, Qingbo Shu, Peibin Liu, Lili Niu, Xiaojing Guo, Xiang Ding, Peng Xue, Zhensheng Xie, Jifeng Wang, Nali Zhu, Peng Wu, Lili Niu, Fuquan Yang
Phospholipids (PLs), one of the lipid categories, are not only the primary building blocks of cellular membranes, but also can be split to produce products that function as second messengers in signal transduction and play a pivotal role in numerous cellular processes, including cell growth, survival, and motility. Here, we present an integrated novel method that combines a fast and robust TMS-diazomethane-based phosphate derivatization and isotopic labeling strategy, which enables simultaneous profiling and relative quantification of PLs from biological samples...
March 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/26710457/-acetylated-histone-expressions-of-the-primary-hippocampal-neurons-in-rats-reduced-by-sicbp-lentivirus
#19
Nali Hou, Xiaofeng Wu, Lan Ren, Min Guo, Yang Bi, Youxue Liu, Jie Chen, Hongmei Haung, Tingyu Li
This study aims to construct the recombinant lentivirus vector containing specific small interfering RNA (siRNA) targeting rat CREB binding protein(CBP)gene and to identify its function of inhibiting the expressions of acetylated histone in primarily cultured hippocampal neurons. Firstly, we constructed four kinds of recombinant lentivirus siCBP. And then we used them to infect the primarily cultured hippocampal neurons, and performed real-time PCR, western blot respectively to detect the expressions of CBP...
August 2015: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/26708765/demethylation-of-methylmercury-in-growing-rice-plants-an-evidence-of-self-detoxification
#20
Xiaohan Xu, Jiating Zhao, Yunyun Li, Yuqin Fan, Nali Zhu, Yuxi Gao, Bai Li, Hanyu Liu, Yu-Feng Li
Mercury (Hg) is a global pollutant that poses a serious threat to human and the environment. Rice was found as an important source for human exposure to Hg in some areas. In this study, the transportation and transformation of IHg and MeHg in rice plants exposed to IHg or MeHg were investigated. The IHg and MeHg concentrations in rice roots and shoots collected every five days were analyzed by HPLC-ICP-MS and SR-XANES. When exposed to MeHg, the percent of IHg in rice roots and shoots increased while MeHg decreased significantly, suggesting prominent demethylation of MeHg occurred...
March 2016: Environmental Pollution
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