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https://www.readbyqxmd.com/read/28636181/identification-of-biomarkers-for-early-diagnosis-of-acute-myocardial-infarction
#1
Wen-Han Ge, Yong Lin, Sen Li, Xuefeng Zong, Zhong-Chun Ge
Acute myocardial infarction (AMI) is a common disease with serious consequences in mortality and cost. Here we aim to screen the differentially expressed genes (DEGs) as biomarkers for early diagnosis of AMI. The microarray data of AMI was downloaded from Gene Expression Omnibus (GEO), including two independent types of research GSE66360 and GSE62646. The DEGs between control and processed samples were screened out by using limma package. Meanwhile, we performed functional analysis of GO terms and/or KEGG pathways to observe the enriched pathways of the DEGs...
June 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28636123/sem-imaging-of-the-stimulatory-response-of-raw264-7-cells-against-porphyromonas-gingivalis-using-a-simple-technique-employing-new-conductive-materials
#2
Chisato Takahashi, Yoshiki Umemura, Ayako Naka, Hiromitsu Yamamoto
In the medical biology, it is essential to understand not only biological morphology but also the interaction between biological materials and agents. To study these, electron microscopy (EM) is often utilized. However, sample preparation techniques for EM require a high level of skill and a considerable time. Here, we conducted EM using a simple technique employing a conductive liquid, BEL-1, and compared the results with another simple technique employing an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4 ])...
June 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28634640/the-microbiome-of-eucalyptus-roots-under-different-management-conditions-and-its-potential-for-biological-nitrogen-fixation
#3
Eduardo da Siva Fonseca, Raquel Silva Peixoto, Alexandre Soares Rosado, Fabiano de Carvalho Balieiro, James M Tiedje, Caio Tavora Coelho da Costa Rachid
Eucalyptus plantations offer a cost-effective and renewable source of raw material. There is substantial interest in improving forestry production, especially through sustainable strategies such as the use of plant growth-promoting bacteria. However, little is known about Eucalyptus microbiology. In this study, the endophytic bacterial community was assessed in Eucalyptus urograndis roots using culture-dependent and culture-independent techniques with plants grown under different conditions. Three phyla accounted for approximately 95% of the community, with Actinobacteria corresponding to approximately 59%...
June 21, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28634340/dispersal-ability-determines-the-scaling-properties-of-species-abundance-distributions-a-case-study-using-arthropods-from-the-azores
#4
Luís Borda-de-Água, Robert J Whittaker, Pedro Cardoso, François Rigal, Ana M C Santos, Isabel R Amorim, Aristeidis Parmakelis, Kostas A Triantis, Henrique M Pereira, Paulo A V Borges
Species abundance distributions (SAD) are central to the description of diversity and have played a major role in the development of theories of biodiversity and biogeography. However, most work on species abundance distributions has focused on one single spatial scale. Here we used data on arthropods to test predictions obtained with computer simulations on whether dispersal ability influences the rate of change of SADs as a function of sample size. To characterize the change of the shape of the SADs we use the moments of the distributions: the skewness and the raw moments...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28633911/fate-of-metals-and-emissions-of-organic-pollutants-from-torrefaction-of-waste-wood-msw-and-rdf
#5
Mar Edo, Nils Skoglund, Qiuju Gao, Per-Erik Persson, Stina Jansson
Torrefaction of municipal solid waste (MSW), refuse-derived fuel (RDF), and demolition and construction wood (DC) was performed at 220°C and a residence time of 90min in a bench-scale reactor. The levels of toxic polychlorinated dibenzo-p-dioxins (PCDD) and dibenzofurans (PCDF) contained in emission from the torrefaction process were evaluated. In addition, main ash-forming elements and trace metals in the raw feedstock and char were determined. The use of MSW in fuel blends with DC resulted in lower PCDD and PCDF emissions after torrefaction, compared with the RDF blends...
June 17, 2017: Waste Management
https://www.readbyqxmd.com/read/28633860/influencing-factors-and-kinetics-analysis-on-the-leaching-of-iron-from-boron-carbide-waste-scrap-with-ultrasound-assisted-method
#6
REVIEW
Xin Li, Pengfei Xing, Xinghong Du, Shuaibo Gao, Chen Chen
In this paper, the ultrasound-assisted leaching of iron from boron carbide waste-scrap was investigated and the optimization of different influencing factors had also been performed. The factors investigated were acid concentration, liquid-solid ratio, leaching temperature, ultrasonic power and frequency. The leaching of iron with conventional method at various temperatures was also performed. The results show the maximum iron leaching ratios are 87.4%, 94.5% for 80min-leaching with conventional method and 50min-leaching with ultrasound assistance, respectively...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633611/rawification-and-the-careful-generation-of-open-government-data
#7
Jérôme Denis, Samuel Goëta
Drawing on a two-year ethnographic study within several French administrations involved in open data programs, this article aims to investigate the conditions of the release of government data - the rawness of which open data policies require. This article describes two sets of phenomena. First, far from being taken for granted, open data emerge in administrations through a progressive process that entails uncertain collective inquiries and extraction work. Second, the opening process draws on a series of transformations, as data are modified to satisfy an important criterion of open data policies: the need for both human and technical intelligibility...
June 1, 2017: Social Studies of Science
https://www.readbyqxmd.com/read/28633605/controlling-response-dependence-in-the-measurement-of-change-using-the-rasch-model
#8
David Andrich
The advantages of using person location estimates from the Rasch model over raw scores for the measurement of change using a common test include the linearization of scores and the automatic handling of statistical properties of repeated measurements. However, the application of the model requires that the responses to the items are statistically independent in the sense that the specific responses to the items on the first time of testing do not affect the responses at a second time. This requirement implies that the responses to the items at both times of assessment are governed only by the invariant location parameters of the items at the two times of testing and the location parameters of each person each time...
January 1, 2017: Statistical Methods in Medical Research
https://www.readbyqxmd.com/read/28632436/development-of-computer-algorithm-for-editing-of-next-generation-sequencing-metagenome-data
#9
Radhika Khanna, Sangeeta Mittal, Sujata Mohanty
The successful implementation of the advanced sequencing technology, the next generation sequencing (NGS) motivates scientists from diverse fields of biological research especially from genomics and transcriptomics in generating large genomic data set to make their analysis more robust and come up with strong inference. However, exploiting this huge genomic data set becomes a challenge for the molecular biologists. To corroborate this problem, computational software and hardware are being developed in parallel and become an integral part of life science...
June 20, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/28631833/tools-to-minimize-inter-laboratory-variability-in-vitellogenin-gene-expression-monitoring-programs
#10
Aaron Jastrow, Denise A Gordon, Kasie M Auger, Elizabeth C Punska, Kathleen F Arcaro, Kristen Keteles, Dana Winkelman, David Lattier, Adam Biales, James M Lazorchak
The egg yolk precursor protein vitellogenin is widely used as a biomarker of estrogen exposure in male fish. However, standardized methodology is lacking and little is known regarding the reproducibility of results among laboratories using different equipment, reagents, protocols, and employing different data analysis programs. To address this data gap we tested the reproducibility across labs to evaluate vitellogenin gene (vtg) expression and assessed the value of using a freely available software data analysis program...
June 20, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28631829/relationship-of-human-herpes-virus-6-and-multiple-sclerosis-a-systematic-review-and-meta-analysis
#11
REVIEW
Ali Pormohammad, Taher Azimi, Fateme Falah, Ebrahim Faghihloo
Background Infection with human herpes viruses has been suggested to contribute to multiple sclerosis (MS), while interaction between human herpes 6 (HHV6) and MS remain unclear yet. Here, we conducted a meta-analysis on the relationship of HHV6 infection and MS. Materials and Methods All related studies were collected from major databases. The analyses were performed by STATA 14 and Comprehensive Meta-Analysis V2.0 softwares. Pooled odds ratios (ORs) and 95% CIs were calculated from the raw data of the including studies by the random effects models when I2 >50% and fix model when I2 < 50%...
June 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28631590/comparative-evaluation-of-veriflow-%C3%A2-salmonella-species-to-usda-and-fda-culture-based-methods-for-the-detection-of-salmonella-spp-in-food-and-environmental-samples
#12
Amrita Puri, Adam C Joelsson, Shawn P Terkhorn, Ashley S Brown, Zara E Gaudioso, Nicholas A Siciliano
Veriflow(®)Salmonella species (Veriflow SS)is a molecular-based assay for the presumptive detection of Salmonella spp. from environmental surfaces (stainless steel, sealed concrete, plastic, and ceramic tile), dairy (2% milk), raw meat (20% fat ground beef), chicken carcasses, and ready-to-eat (RTE) food (hot dogs). The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 min post-PCR amplification and requires only an 18 h enrichment for maximum sensitivity...
June 20, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28631204/adaptive-step-size-lms-improves-ecg-detection-during-mri-at-1-5-and-3%C3%A2-t
#13
André Guillou, Jean-Marc Sellal, Sarah Ménétré, Grégory Petitmangin, Jacques Felblinger, Laurent Bonnemains
OBJECTIVE: We describe a new real-time filter to reduce artefacts on electrocardiogram (ECG) due to magnetic field gradients during MRI. The proposed filter is a least mean square (LMS) filter able to continuously adapt its step size according to the gradient signal of the ongoing MRI acquisition. MATERIALS AND METHODS: We implemented this filter and compared it, within two databases (at 1.5 and 3 T) with over 6000 QRS complexes, to five real-time filtering strategies (no filter, low pass filter, standard LMS, and two other filters optimized within the databases: optimized LMS, and optimized Kalman filter)...
June 19, 2017: Magma
https://www.readbyqxmd.com/read/28631125/upgraded-modified-forms-of-bituminous-coal-for-the-removal-of-safranin-t-dye-from-aqueous-solution
#14
Mohamed Shaban, Mostafa R Abukhadra, Mohamed G Shahien, Aftab Aslam Parwaz Khan
Natural bituminous coal was used as a precursor in the synthesis of different modified products. The modification of coal was performed by treating it with nitric acid (N-coal), coating its surface by zinc oxide nanoparticles (Z-coal), and converting it into porous graphite (PG). The effect of modification processes on the structures, morphologies, and optical properties was followed by X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectrum (FT-IR), and UV/VIS spectrophotometer analysis...
June 19, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28631049/novel-probiotics-isolated-from-a-japanese-traditional-fermented-food-funazushi-attenuates-dss-induced-colitis-by-increasing-the-induction-of-high-integrin-%C3%AE-v-%C3%AE-8-expressing-dendritic-cells
#15
Yoshikiyo Okada, Yoshikazu Tsuzuki, Takajo Takeshi, Hirotaka Furuhashi, Masaaki Higashiyama, Chikako Watanabe, Kazuhiko Shirakabe, Chie Kurihara, Shunsuke Komoto, Kengo Tomita, Shigeaki Nagao, Soichiro Miura, Ryota Hokari
BACKGROUND: We isolated two novel probiotics strains (s193 and s292) from Funazushi, which is a traditional Japanese fermented food, and evaluated its effects on DSS-induced colitis to determine the possible underlying mechanisms. METHODS: A single colony from homogenized Funazushi was isolated by its ability to suppress TNF-α in RAW 264.7. Effect of probiotics on colonic inflammation induced by DSS was evaluated. Effect of probiotics on Treg induction by CD11c(+) dendritic cells (DCs) of MLNs were analyzed...
June 19, 2017: Journal of Gastroenterology
https://www.readbyqxmd.com/read/28630944/clinvar-data-parsing
#16
Xiaolei Zhang, Eric V Minikel, Anne H O'Donnell-Luria, Daniel G MacArthur, James S Ware, Ben Weisburd
This software repository provides a pipeline for converting raw ClinVar data files into analysis-friendly tab-delimited tables, and also provides these tables for the most recent ClinVar release. Separate tables are generated for genome builds GRCh37 and GRCh38 as well as for mono-allelic variants and complex multi-allelic variants. Additionally, the tables are augmented with allele frequencies from the ExAC and gnomAD datasets as these are often consulted when analyzing ClinVar variants. Overall, this work provides ClinVar data in a format that is easier to work with and can be directly loaded into a variety of popular analysis tools such as R, python pandas, and SQL databases...
2017: Wellcome Open Research
https://www.readbyqxmd.com/read/28630784/elemental-characterization-of-romanian-crop-medicinal-plants-by-neutron-activation-analysis
#17
Daniela Haidu, Dénes Párkányi, Radu Ioan Moldovan, Cecilia Savii, Iulia Pinzaru, Cristina Dehelean, Ludovic Kurunczi
The metallic elements concentrations of medicinal plants (coriander, dill, Echinacea, lavender, chamomile, mint, and plantain, used for phytopharmaceutical products), cultivated in unpolluted region, were analyzed by neutron activation analysis. The essential nutrients, macro-, micro-, and trace elements (K, Ca, Mg, Na, Fe, Mn, Rb, Sr, and Zn), potentially toxic elements (Al, As, Ba, Co, Sb, Cr, and V), and rare earth elements were monitored and were compared with those presented in the literature. An estimation of their contributions to intake and toxicity for a person was made, which revealed that (a) teas prepared from the examined plants represent useful contribution to the food provided intake of three essential macronutrients (K, Ca, and Mg); (b) the Cu, Mn, Rb, Sr, Zn, and rare earths levels are normal or low; (c) the quantities of As, Ba, Co, Sb, Cr, and V do not represent toxicological concerns; (d) the examination of the estimated Al and Fe quantities recovered in infusions in the conditions of usual daily tea consumption is below the Tolerable Daily Intake values...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28630681/imaging-optical-scattering-of-butterfly-wing-scales-with-a-microscope
#18
Jinxin Fu, Beom-Jin Yoon, Jung Ok Park, Mohan Srinivasarao
A new optical method is proposed to investigate the reflectance of structurally coloured objects, such as Morpho butterfly wing scales and cholesteric liquid crystals. Using a reflected-light microscope and a digital single-lens reflex (DSLR) camera, we have successfully measured the two-dimensional reflection pattern of individual wing scales of Morpho butterflies. We demonstrate that this method enables us to measure the bidirectional reflectance distribution function (BRDF). The scattering image observed in the back focal plane of the objective is projected onto the camera sensor by inserting a Bertrand lens in the optical path of the microscope...
August 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/28629726/th2-related-immune-responses-by-the-brucella-abortus-cellular-antigens-malate-dehydrogenase-elongation-factor-and-arginase
#19
Young Bin Im, Soojin Shim, Woo Bin Park, Suk Kim, Han Sang Yoo
Brucellosis is an important zoonotic disease caused by Brucella species. The disease is difficult to control due to the intracellular survival of the bacterium and the lack of precise understanding of pathogenesis. Despite of continuous researches on the pathogenesis of Brucella spp. infection, there is still question on the pathogenesis, especially earlier immune response in the bacterial infection. Malate dehydrogenase (MDH), elongation factor (Tsf), and arginase (RocF), which showed serological reactivity, were purified after gene cloning, and their immune modulating activities were then analyzed in a murine model...
June 16, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28629537/triterpenoid-saponins-from-the-roots-of-cyathula-officinalis-and-their-inhibitory-effects-on-nitric-oxide-production
#20
Yun-Tao Jiang, Wen-Jing Yan, Chu-Lu Qi, Ji-Qin Hou, Yan-Ying Zhong, Hui-Jun Li, Hao Wang, Ping Li
The present study was designed to investigate the chemical constituents of the roots of Cyathula officinalis. Compounds were isolated by silica gel, Sephadex LH-20, ODS column chromatography, and preparative HPLC. Their structures were determined on the basis of 1D and 2D NMR techniques, mass spectrometry, and chemical methods. One new oleanane-type triterpenoid saponin, 28-O-[α-L-rhamnopyranosyl-(1→3)-β-D-glucuronopyranosyl-(1→3)-β-D-glucopyranosyl] hederagenin (1), was isolated from the roots of Cyathula officinalis...
June 2017: Chinese Journal of Natural Medicines
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