Elizabeth A Wynn, Christian Dide-Agossou, Matthew Reichlen, Karen Rossmassler, Reem Al Mubarak, Justin J Reid, Samuel T Tabor, Sarah E M Born, Monica R Ransom, Rebecca M Davidson, Kendra N Walton, Jeanne B Benoit, Amanda Hoppers, Dorothy E Loy, Allison A Bauman, Lisa M Massoudi, Gregory Dolganov, Michael Strong, Payam Nahid, Martin I Voskuil, Gregory T Robertson, Camille M Moore, Nicholas D Walter
To address the ongoing global tuberculosis crisis, there is a need for shorter, more effective treatments. A major reason why tuberculosis requires prolonged treatment is that, following a short initial phase of rapid killing, the residual Mycobacterium tuberculosis withstands drug killing. Because existing methods lack sensitivity to quantify low-abundance mycobacterial RNA in drug-treated animals, cellular adaptations of drug-exposed bacterial phenotypes in vivo remain poorly understood. Here, we used a novel RNA-seq method called SEARCH-TB to elucidate the Mycobacterium tuberculosis transcriptome in mice treated for up to 28 days with standard doses of isoniazid, rifampin, pyrazinamide, and ethambutol...
October 31, 2023: MBio