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Bacterial fatty acids

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https://www.readbyqxmd.com/read/28088639/docosahexaenoic-acid-loaded-lipid-nanoparticles-with-bactericidal-activity-against-helicobacter-pylori
#1
Catarina Leal Seabra, Cláudia Nunes, Maria Gomez-Lazaro, Marta Correia, José Carlos Machado, Inês C Gonçalves, Celso A Reis, Salette Reis, M Cristina L Martins
Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid present in fish oil, has been described as a promising molecule to the treatment of Helicobacter pylori gastric infection. However, due to its highly unsaturated structure, DHA can be easily oxidized loosing part of its bioactivity. This work aims the nanoencapsulation of DHA to improve its bactericidal efficacy against H. pylori. DHA was loaded into nanostructured lipid carriers (NLC) produced by hot homogenization and ultrasonication using a blend of lipids (Precirol ATO5(®), Miglyol-812(®)) and a surfactant (Tween 60(®))...
January 11, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28081527/enhanced-microalgal-biomass-and-lipid-production-from-a-consortium-of-indigenous-microalgae-and-bacteria-present-in-municipal-wastewater-under-gradually-mixotrophic-culture-conditions
#2
Hyun Uk Cho, Young Mo Kim, Jong Moon Park
The goal of this study was to investigate the influences of gradually mixotrophic culture conditions on microalgal biomass and lipid production by a consortium of indigenous microalgae and bacteria present in raw municipal wastewater. Lab-scale photobioreactors containing the consortium were operated in repeated batch mode. Initial cultivation (phase I) was performed using only the municipal wastewater, then 10% and 25% of the reactor volumes were replaced with the effluent from a sewage sludge fermentation system producing volatile fatty acids (SSFV) at the beginnings of phase II and phase III, respectively...
December 25, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/28070968/bacterial-short-chain-fatty-acid-metabolites-modulate-the-inflammatory-response-against-infectious-bacteria
#3
R O Corrêa, A Vieira, E M Sernaglia, M Lancellotti, A T Vieira, M J Avila-Campos, H G Rodrigues, M A R Vinolo
Short chain fatty acids (SCFAs), predominately acetic, propionic and butyric acids, are bacterial metabolites with an important role in the maintenance of homeostasis due to their metabolic and immunomodulatory actions. Some evidence suggests that they may also be relevant during infections. Therefore, we aimed to investigate the effects of SCFAs in the effector functions of neutrophils to an opportunistic pathogenic bacteria, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Using a subcutaneous model to generate a mono, isolated infection of A...
January 9, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28069689/comparative-evaluation-of-raw-and-ripe-fruits-of-forsythia-suspensa-by-hplc-esi-ms-ms-analysis-and-anti-microbial-assay
#4
Jialin Qu, Xinjia Yan, Chunyan Li, Jing Wen, Chongning Lu, Jungang Ren, Ying Peng, Shaojiang Song
A multi-component quantification fingerprint based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry technique has been established for the comparative analysis of raw and ripe fruits of Forsythia suspensa originated from different provinces. Eighteen bioactive constituents including three phenylethanoid glycosides derivatives, six phenolic acids, three flavonoids, four phenylpropanoids, one fatty acid and one terpenoid were identified and quantified. Total contents of phenylethanoid glycosides, phenylpropanoids and flavonoids from raw samples were found much higher than those from ripe samples, while total content of phenolic acids showed a contrary tendency...
January 8, 2017: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/28069576/dysbiosis-and-zonulin-upregulation-alter-gut-epithelial-and-vascular-barriers-in-patients-with-ankylosing-spondylitis
#5
Francesco Ciccia, Giuliana Guggino, Aroldo Rizzo, Riccardo Alessandro, Michele Maria Luchetti, Simon Milling, Laura Saieva, Heleen Cypers, Tommaso Stampone, Paola Di Benedetto, Armando Gabrielli, Alessio Fasano, Dirk Elewaut, Giovanni Triolo
BACKGROUND: Dysbiosis has been recently demonstrated in patients with ankylosing spondylitis (AS) but its implications in the modulation of intestinal immune responses have never been studied. The aim of this study was to investigate the role of ileal bacteria in modulating local and systemic immune responses in AS. METHODS: Ileal biopsies were obtained from 50 HLA-B27(+) patients with AS and 20 normal subjects. Silver stain was used to visualise bacteria. Ileal expression of tight and adherens junction proteins was investigated by TaqMan real-time (RT)-PCR and immunohistochemistry...
January 9, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/28068215/tibeticola-sediminis-gen-nov-sp-nov-a-thermophilic-bacterium-isolated-from-hot-spring
#6
Inam Ullah Khan, Firasat Hussain, Ye Tian, Neeli Habib, Wen-Dong Xian, Zhao Jiang, Arshia Amin, Chang-Guo Yuan, En-Min Zhou, Xiao-Yang Zhi, Wen-Jun Li
Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008 were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. Taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-staining negative, aerobic, short rod shaped and motile by means of a polar flagellum. They were oxidase and catalase positive and were able to grow at 30-55 °C (optimum, 37-45 °C), pH 6.0-8.0 (optimum, pH 7...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28068214/paenibacillus-rhizoplanae-sp-nov-isolated-from-the-rhizosphere-of-zea-mays
#7
Peter Kämpfer, Hans-Jürgen Busse, John A McInroy, Chia-Hui Hu, Joseph W Kloepper, Stefanie P Glaeser
A Gram-positive staining, aerobic, endospore-forming bacterial strain, isolated from the rhizosphere of Zea mays was studied for its detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-64T was shown to be a member of the genus Paenibacillus, most closely related to the type strains of Paenibacillus silagei (99%) and Paenibacillus borealis (97.5%). The 16S rRNA gene sequence similarity to all other Paenibacillus species was ≤ 97.5%. DNA-DNA hybridization values to the type strains of P...
January 6, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28065211/faecal-parameters-as-biomarkers-of-the-equine-hindgut-microbial-ecosystem-under-dietary-change
#8
P Grimm, C Philippeau, V Julliand
Faeces could be used for evaluating the balance of the equine hindgut microbial ecosystem, which would offer a practical method for assessing gut health and how this relates to disease. However, previous studies concluded that faeces microbial ecosystem was not representative of the proximal hindgut (caecum and ventral colon). This study aimed to evaluate if variations of the faecal microbial ecosystem were similar to those observed in the proximal hindgut. Six horses, fistulated in the caecum and right ventral (RV) colon, were subjected to a gradual change of diet, from a 100% hay (high fibre) diet (2...
January 9, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28063283/microbial-community-redundancy-in-anaerobic-digestion-drives-process-recovery-after-salinity-exposure
#9
Jo De Vrieze, Marlies E R Christiaens, Diego Walraedt, Arno Devooght, Umer Zeeshan Ijaz, Nico Boon
Anaerobic digestion of high-salinity wastewaters often results in process inhibition due to the susceptibility of the methanogenic archaea. The ability of the microbial community to deal with increased salinity levels is of high importance to ensure process perseverance or recovery after failure. The exact strategy of the microbial community to ensure process endurance is, however, often unknown. In this study, we investigated how the microbial community is able to recover process performance following a disturbance through the application of high-salinity molasses wastewater...
December 26, 2016: Water Research
https://www.readbyqxmd.com/read/28056226/flectobacillus-pallidus-sp-nov-isolated-from-a-spring
#10
Shih-Yi Sheu, Li-Pin Liu, Wen-Ming Chen
A novel bacterial strain MVW-6T was isolated from a spring in Taiwan and characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MVW-6T belonged to the genus Flectobacillus and showed the highest levels of sequence similarity with respect to Flectobacillus lacus CL-GP79T (98.2 %) and Flectobacillus fontis MIB-4T (97.4 %). Cells of strain MVW-6T were Gram-staining-negative, aerobic, non-motile rods that were covered by large capsules and formed light pink-colored colonies...
January 5, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28056225/xenorhabdus-thuongxuanensis-sp-nov-and-xenorhabdus-eapokensis-sp-nov-isolated-from-steinernema-species
#11
Peter Kämpfer, Nicholas J Tobias, Long Phan Ke, Helge B Bode, Stefanie P Glaeser
Two slightly yellowish pigmented, oxidase-negative, rod-shaped and Gram-negative bacterial strains (30TX1T and DL20T), isolated from Steinernema sangi and Steinernema eapokense, respectively, during soil sampling in Vietnam were studied in a polyphasic taxonomic approach. Strain 30TX1T showed highest 16S rRNA gene sequence similarity to Xenorhabdus ehlersii (98.9%) and strain DL20T to Xenorhabdus ishibashii (98.7%). Sequence similarities to all other Xenorhabdus species were lower (< 98.4%). The two strains shared 98% 16S rRNA gene sequence similarity...
January 4, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28056221/nitratireductor-aestuarii-sp-nov-a-marine-alphaproteobacterium-isolated-from-estuary-of-jiulong-river-of-southeast-of-china
#12
Danyun Ou, Hao Huang, Renao Bai, Qingge Li, Yanan Wang, Yajie Yin
Two bacterial strains 2-2-12-1T and 2-2-12-2 were isolated from the estuary of the Jiulong River of southeast of China. The cells were Gram-negative, non-motile, short rods without flagella. Growth occurred at 25-45 ºC, pH 5.0-9.0, and 0.5-2% NaCl. The bacteria were unable to reduce nitrate. The predominant fatty acids were C19: 0 cyclo ω9c and C18: 1 ω7c. Phylogenetic analysis based on the 16S rRNA gene sequences showed that both strains belong to the genus Nitratireductor, family Phyllobacteriaceae, class Alphaproteobacteria...
January 4, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28054497/the-lipopolysaccharide-lipid-a-long-chain-fatty-acid-is-important-for-rhizobium-leguminosarum-growth-and-stress-adaptation-in-free-living-and-nodule-environments
#13
Dianna V Bourassa, Elmar Kannenberg, Janine Sherrier, Richard Jeffrey Buhr, Russell Carlson
Rhizobium bacteria live in soil and plant environments, are capable of inducing symbiotic nodules on legumes, invade these nodules, and develop into bacteroids that fix atmospheric nitrogen into ammonia. Rhizobial lipopolysaccharide (LPS) is anchored in the bacterial outer membrane through a specialized lipid A containing a very long chain fatty acid (VLCFA). VLCFA function for rhizobial growth in soil and plant environments is not well understood. Two genes, acpXL and lpxXL, encoding acyl carrier protein and acyltransferase, are among the six genes required for biosynthesis and transfer of VLCFA to lipid A...
January 5, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28052072/microbial-response-to-soil-liming-of-damaged-ecosystems-revealed-by-pyrosequencing-and-phospholipid-fatty-acid-analyses
#14
Ramya Narendrula-Kotha, Kabwe K Nkongolo
AIMS: To assess the effects of dolomitic limestone applications on soil microbial communities' dynamics and bacterial and fungal biomass, relative abundance, and diversity in metal reclaimed regions. METHODS AND RESULTS: The study was conducted in reclaimed mining sites and metal uncontaminated areas. The limestone applications were performed over 35 years ago. Total microbial biomass was determined by Phospholipid fatty acids. Bacterial and fungal relative abundance and diversity were assessed using 454 pyrosequencing...
2017: PloS One
https://www.readbyqxmd.com/read/28050852/microbial-biomass-in-compost-during-colonization-of-agaricus-bisporus
#15
Aurin M Vos, Amber Heijboer, Henricus T S Boschker, Barbara Bonnet, Luis G Lugones, Han A B Wösten
Agaricus bisporus mushrooms are commercially produced on a microbe rich compost. Here, fungal and bacterial biomass was quantified in compost with and without colonization by A. bisporus. Chitin content, indicative of total fungal biomass, increased during a 26-day period from 576 to 779 nmol N-acetylglucosamine g(-1) compost in the absence of A. bisporus (negative control). A similar increase was found in the presence of this mushroom forming fungus. The fungal phospholipid-derived fatty acid (PLFA) marker C18:2ω6, indicative of the living fraction of the fungal biomass, decreased from 575 to 280 nmol g(-1) compost in the negative control...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28045020/structural-characterisation-of-the-fatty-acid-biosynthesis-enzyme-fabf-from-the-pathogen-listeria-monocytogenes
#16
Tatiana P Soares da Costa, Jeffrey D Nanson, Jade K Forwood
Development of new antimicrobial agents is required against the causative agent for listeriosis, Listeria monocytogenes, as the number of drug resistant strains continues to increase. A promising target is the β-ketoacyl-acyl carrier protein synthase FabF, which participates in the catalysis of fatty acid synthesis and elongation, and is required for the production of phospholipid membranes, lipoproteins, and lipopolysaccharides. In this study, we report the 1.35 Å crystal structure of FabF from L. monocytogenes, providing an excellent platform for the rational design of novel inhibitors...
January 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28044276/antimicrobial-potential-and-taxonomic-investigation-of-piezotolerant-streptomyces-sp-niot-ch-40-isolated-from-deep-sea-sediment
#17
Vishnu Priya Padmanaban, Pankaj Verma, Srividhyalakshmi Venkatabaskaran, Thirupathi Keppayan, Dharani Gopal, Ashok Kumar Sekar, Kirubagaran Ramalingam
Microbial-derived natural products from extreme niches such as deepsea are known to possess structural and functional novelty. With this background, the present study was designed to investigate the bioprospecting potential and systematics of a deep-sea derived piezotolerant bacterial strain NIOT-Ch-40, showing affiliation to the genus Streptomyces based on 16S RNA gene similarity. Preliminary screening for the presence of biosynthetic genes like polyketide synthase I, polyketide synthase II, non ribosomal peptide synthase, 3-amino-5-hydroxybenzoic acid synthase and spiroindimicin followed by antibacterial activity testing confirmed the presence of potent bioactivity...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28032540/photorhabdus-luminescens-subsp-namnaonensis-subsp-nov-isolated-from-heterorhabditis-baujardi-nematodes-in-nam-nao-district-of-central-thailand
#18
Stefanie P Glaeser, Nicholas J Tobias, Aunchalee Thanwisai, Narisara Chantratita, Helge B Bode, Peter Kämpfer
A lightly yellowish pigmented, oxidase-negative bacterial strain (PB45.5T), isolated from the Nam Nao district of Phetchabun in central Thailand was investigated for its taxonomic position. The cells morphology of the isolate showed a rod shaped appearance. The strain stained Gram-negative. Strain PB45.5T shared highest 16S rRNA gene sequence similarity with the type strain of Photorhabdus luminescens subsp. akhurstii (99.2%) and Photorhabdus luminescens subsp. hainanensisi (99.1%) and lower similarities to all other Photorhabdus luminescens subspecies (< 98...
December 29, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28028566/lacinutrix-chionocetis-sp-nov-isolated-from-gut-of-a-red-snow-crab
#19
Hyangmi Kim, Sang-Chul Yoon, Kwang-Ho Choi, Sung-Tae Kim, Jae-Bong Lee, Dong-Sun Kim, Ho Le Han, Kyung Sook Bae, Doo-Sang Park
A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated MAB-07(T), was isolated from the gut of a red snow crab. The novel strain grew optimally at 20 °C, pH 7.0-8.0, and in the presence of 3% (w/v) NaCl. A phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain MAB-07(T) belongs to the type strains of species of the genus Lacinutrix. Strain MAB-07(T) exhibited 16S rRNA gene sequence similarity values of 95.5-97.8% with the type strains of species of the genus Lacinutrix...
December 27, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/28028230/ohr-plays-a-central-role-in-bacterial-responses-against-fatty-acid-hydroperoxides-and-peroxynitrite
#20
Thiago G P Alegria, Diogo A Meireles, José R R Cussiol, Martín Hugo, Madia Trujillo, Marcos Antonio de Oliveira, Sayuri Miyamoto, Raphael F Queiroz, Napoleão Fonseca Valadares, Richard C Garratt, Rafael Radi, Paolo Di Mascio, Ohara Augusto, Luis E S Netto
Organic hydroperoxide resistance (Ohr) enzymes are unique Cys-based, lipoyl-dependent peroxidases. Here, we investigated the involvement of Ohr in bacterial responses toward distinct hydroperoxides. In silico results indicated that fatty acid (but not cholesterol) hydroperoxides docked well into the active site of Ohr from Xylella fastidiosa and were efficiently reduced by the recombinant enzyme as assessed by a lipoamide-lipoamide dehydrogenase-coupled assay. Indeed, the rate constants between Ohr and several fatty acid hydroperoxides were in the 10(7)-10(8) M(-1)⋅s(-1) range as determined by a competition assay developed here...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
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