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https://www.readbyqxmd.com/read/29235272/real-time-detection-of-antibiotic-activity-by-measuring-nanometer-scale-bacterial-deformation
#1
Rafael Iriya, Karan Syal, Wenwen Jing, Manni Mo, Hui Yu, Shelley E Haydel, Shaopeng Wang, Nongjian Tao
Diagnosing antibiotic-resistant bacteria currently requires sensitive detection of phenotypic changes associated with antibiotic action on bacteria. Here, we present an optical imaging-based approach to quantify bacterial membrane deformation as a phenotypic feature in real-time with a nanometer scale (∼9  nm) detection limit. Using this approach, we found two types of antibiotic-induced membrane deformations in different bacterial strains: polymyxin B induced relatively uniform spatial deformation of Escherichia coli O157:H7 cells leading to change in cellular volume and ampicillin-induced localized spatial deformation leading to the formation of bulges or protrusions on uropathogenic E...
December 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29233991/structure-of-outer-membrane-protein-g-in-lipid-bilayers
#2
Joren S Retel, Andrew J Nieuwkoop, Matthias Hiller, Victoria A Higman, Emeline Barbet-Massin, Jan Stanek, Loren B Andreas, W Trent Franks, Barth-Jan van Rossum, Kutti R Vinothkumar, Lieselotte Handel, Gregorio Giuseppe de Palma, Benjamin Bardiaux, Guido Pintacuda, Lyndon Emsley, Werner Kühlbrandt, Hartmut Oschkinat
β-barrel proteins mediate nutrient uptake in bacteria and serve vital functions in cell signaling and adhesion. For the 14-strand outer membrane protein G of Escherichia coli, opening and closing is pH-dependent. Different roles of the extracellular loops in this process were proposed, and X-ray and solution NMR studies were divergent. Here, we report the structure of outer membrane protein G investigated in bilayers of E. coli lipid extracts by magic-angle-spinning NMR. In total, 1847 inter-residue 1H-1H and 13C-13C distance restraints, 256 torsion angles, but no hydrogen bond restraints are used to calculate the structure...
December 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29233892/stable-signal-peptides-and-the-response-to-secretion-stress-in-staphylococcus-aureus
#3
Arryn Craney, Floyd E Romesberg
Protein secretion is essential, but how it is managed is poorly understood. In bacteria, most secreted proteins require release from the outer surface of the cytoplasmic membrane by type I signal peptidase (SPase), which cleaves the mature protein from its membrane-bound N-terminal signal peptide. As the first step that occurs outside the protected cytoplasmic environment and because insufficient activity can rapidly result in the toxic accumulation of preproteins, the activity of SPase is expected to be closely monitored and perhaps supplemented when insufficient...
December 12, 2017: MBio
https://www.readbyqxmd.com/read/29230232/chemosensitization-of-fusarium-graminearum-to-chemical-fungicides-using-cyclic-lipopeptides-produced-by-bacillus-amyloliquefaciens-strain-jck-12
#4
Kihyun Kim, Yoonji Lee, Areum Ha, Ji-In Kim, Ae Ran Park, Nan Hee Yu, Hokyoung Son, Gyung Ja Choi, Hae Woong Park, Chul Won Lee, Theresa Lee, Yin-Won Lee, Jin-Cheol Kim
Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29229778/structural-and-functional-insights-into-the-role-of-bamd-and-bame-within-the-%C3%AE-barrel-assembly-machinery-in-neisseria-gonorrhoeae
#5
Aleksandra E Sikora, Igor H Wierzbicki, Ryszard A Zielke, Rachael F Ryner, Konstantin V Korotkov, Susan K Buchanan, Nicholas Noinaj
The β-barrel assembly machinery (BAM) is a conserved multi-component protein complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. Given its role in the production of OMPs for survival and pathogenesis, BAM represents an attractive target for the development of therapeutic interventions including drugs and vaccines against multi-drug resistant bacteria such as Neisseria gonorrhoeae The first structure of BamA, the central component of BAM, was from N...
December 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29229504/an-inhibitory-action-of-chitosan-nanoparticles-against-pathogenic-bacteria-and-fungi-and-their-potential-applications-as-biocompatible-antioxidants
#6
D MubarakAli, F LewisOscar, V Gopinath, Naify S Alharbi, Sulaiman Ali Alharbi, N Thajuddin
Chitosan is the second most abundant polymer obtained from the byproduct of seafood. Chitosan and its derivatives and chitosan loaded drugs are the recent area of interest against microbial pathogenesis. The cationic chitosan nanoparticles (ChNPs) interact with the anionic surfaces of the microbial cell membrane, which promotes antimicrobial activity. Although, ChNPs are potential against pathogenic microbes, selection of adaptable, suitable and cost effective synthesis method is much important. In the present study, ChNPs were synthesized adopting ionic gelation using sodium tripolyphosphate as a cross linking agent and characterized by FTIR, DLS, SEM and TEM analysis...
December 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29227933/synthesis-biological-evaluation-and-metabolic-stability-of-phenazine-derivatives-as-antibacterial-agents
#7
Maddeboina Krishnaiah, Nathalia Rodrigues de Almeida, Venkatareddy Udumula, Zhongcheng Song, Yashpal Singh Chhonker, Mai M Abdelmoaty, Valter Aragao do Nascimento, Daryl J Murry, Martin Conda-Sheridan
Drug-resistant pathogens are a major cause of hospital- and community-associated bacterial infections in the United States and around the world. These infections are increasingly difficult to treat due to the development of antibiotic resistance and the formation of bacterial biofilms. In the paper, a series of phenazines were synthesized and evaluated for their in vitro antimicrobial activity against Gram positive (methicillin resistant staphylococcus aureus, MRSA) and Gram negative (Escherichia coli, E. coli) bacteria...
November 9, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29227931/synthesis-and-antibacterial-evaluation-of-novel-cationic-chalcone-derivatives-possessing-broad-spectrum-antibacterial-activity
#8
Wen-Chao Chu, Peng-Yan Bai, Zhao-Qing Yang, De-Yun Cui, Yong-Gang Hua, Yi Yang, Qian-Qian Yang, En Zhang, Shangshang Qin
There is an urgent need to identify new antibiotics with novel mechanisms that combat antibiotic resistant bacteria. Herein, a series of chalcone derivatives that mimic the essential properties of cationic antimicrobial peptides were designed and synthesized. Antibacterial activities against drug-sensitive bacteria, including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Salmonella enterica, as well as clinical multiple drug resistant isolates of methicillin-resistant S. aureus (MRSA), KPC-2-producing and NDM-1-producing Carbapenem-resistant Enterobacteriaceae were evaluated...
December 5, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29221882/effective-inhibition-of-cbf-14-against-cryptococcus-neoformans-infection-in-mice-and-its-related-anti-inflammatory-activity
#9
Changzhong Yu, Shanshan Wei, Xiaorong Han, Hanhan Liu, Mengxiao Wang, Meiling Jiang, Min Guo, Jie Dou, Changlin Zhou, Lingman Ma
Cbf-14 (RLLRKFFRKLKKSV), a designed peptide derived from cathelicidin family AMP, has proven to be potent against drug-resistant bacteria. In the present study, we investigated the anti-cryptococcal activity of Cbf-14 in vitro and in a pulmonary infection mouse model. Sensitivity test indicated that Cbf-14 possessed effective antifungal activity against Cryptococcus neoformans with an MIC of 4-16 µg/ml, and killing experiments showed that fungicidal activity was achieved after only 4 h treatment with Cbf-14 at 4× MIC concentrations in vitro...
December 5, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29219809/the-cell-wall-of-the-filamentous-anoxygenic-phototrophic-bacterium-oscillochloris-trichoides
#10
Olga I Keppen, Ruslan N Ivanovsky, Galina M Streshinskaya, Natalia V Lebedeva, Alexander S Shashkov, Andrey S Dmitrenok, Denis S Grouzdev
The filamentous anoxygenic phototrophic bacterium Oscillochloris trichoides DG-6 has been studied, and it has been shown that there are no lipopolysaccharides on the cell surface. Fatty acids hydroxylated at the C3 position, amino sugars and phosphate-containing compounds characteristic of lipid A have also not been found. The genes encoding for proteins responsible for the synthesis of lipopolysaccharides and the genes for the transport system, usually localized in the outer membrane of Gram-negative bacteria, have not been detected in the genome...
December 8, 2017: Microbiology
https://www.readbyqxmd.com/read/29218056/respiratory-burst-oxidase-homolog-gene-a-is-crucial-for-rhizobium-infection-and-nodule-maturation-and-function-in-common-bean
#11
Manoj-Kumar Arthikala, Jesús Montiel, Rosana Sánchez-López, Noreide Nava, Luis Cárdenas, Carmen Quinto
Reactive oxygen species (ROS) produced by respiratory burst oxidase homologs (RBOHs) regulate numerous plant cell processes, including the symbiosis between legumes and nitrogen-fixing bacteria. Rapid and transient ROS production was reported after Phaseolus vulgaris root hairs were treated with Nod factors, indicating the presence of a ROS-associated molecular signature in the symbiosis signaling pathway. Rboh is a multigene family containing nine members (RbohA-I) in P. vulgaris. RNA interference of RbohB suppresses ROS production and attenuates rhizobial infection thread (IT) progression in P...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29217618/lps-upregulates-palmitoylated-enzymes-of-the-phosphatidylinositol-cycle-an-insight-from-proteomic-studies
#12
Justyna Sobocińska, Paula Roszczenko-Jasińska, Monika Zaręba-Kozioł, Aneta Hromada-Judycka, Orest V Matveichuk, Gabriela Traczyk, Katarzyna Łukasiuk, Katarzyna Kwiatkowska
Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria which induces strong pro-inflammatory reactions of mammals. These processes are triggered upon sequential binding of LPS to CD14, a GPI-linked plasma membrane raft protein, and to the TLR4/MD2 receptor complex. We have found earlier that upon LPS binding CD14 triggers generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a lipid controlling subsequent pro-inflammatory cytokine production. Here we show that stimulation of RAW264 macrophage-like cells with LPS induces global changes of the level of fatty-acylated, most likely palmitoylated, proteins...
December 7, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29215320/early-effects-of-rhodomyrtone-on-membrane-integrity-in-methicillin-resistant-staphylococcus-aureus
#13
Wipawadee Sianglum, Dennapa Saeloh, Pongsri Tongtawe, Natthakul Wootipoom, Nitaya Indrawattana, Supayang Piyawan Voravuthikunchai
Strong evidence of high potency of rhodomyrtone as a promising antibacterial agent against pathogenic gram-positive bacteria has been clearly demonstrated in our previous work. The aim of this study was to provide insight into early action of rhodomyrtone, an acylphloroglucinol, on membrane damage in multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA). Early effects of rhodomyrtone on the bacterial membrane integrity were detected in a time-course study. Flow cytometry revealed a reduction in green fluorescent emission and increase in uptake of propidium iodide in rhodomyrtone-treated bacterial cells in a concentration- and time-dependent manner...
December 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29212086/comparative-analyses-of-transport-proteins-encoded-within-the-genomes-of-bdellovibrio-bacteriovorus-hd100-and-bdellovibrio-exovorus-jss
#14
Fereshteh Heidari Tajabadi, Arturo Medrano-Soto, Masoud Ahmadzadeh, Gholamreza Salehi Jouzani, Milton H Saier
Bdellovibrio, δ-proteobacteria, including B. bacteriovorus (Bba) and B. exovorus (Bex), are obligate predators of other Gram-negative bacteria. While Bba grows in the periplasm of the prey cell, Bex grows externally. We have analyzed and compared the transport proteins of these 2 organisms based on the current contents of the Transporter Classification Database (TCDB; www.tcdb.org). Bba has 103 transporters more than Bex, 50% more secondary carriers, and 3 times as many MFS carriers. Bba has far more metabolite transporters than Bex as expected from its larger genome, but there are 2 times more carbohydrate uptake and drug efflux systems, and 3 times more lipid transporters...
December 7, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29212056/fe3-saturated-montmorillonite-effectively-deactivates-bacteria-in-wastewater
#15
Chao Qin, Chaoqi Chen, Chao Shang, Kang Xia
Existing water disinfection practices often produce harmful disinfection byproducts. The antibacterial activity of Fe3+-saturated montmorillonite was investigated mechanistically using municipal wastewater effluents. Bacterial deactivation efficiency (bacteria viability loss) was 92±0.64% when a secondary wastewater effluent was mixed with Fe3+-saturated montmorillonite for 30min, and further enhanced to 97±0.61% after 4h. This deactivation efficiency was similar to that when the same effluent was UV-disinfected before it exited a wastewater treatment plant...
December 3, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29212037/a-bacterial-receptor-pcrk-senses-the-plant-hormone-cytokinin-to-promote-adaptation-to-oxidative-stress
#16
Fang-Fang Wang, Shou-Ting Cheng, Yao Wu, Bao-Zhen Ren, Wei Qian
Recognition of the host plant is a prerequisite for infection by pathogenic bacteria. However, how bacterial cells sense plant-derived stimuli, especially chemicals that function in regulating plant development, remains completely unknown. Here, we have identified a membrane-bound histidine kinase of the phytopathogenic bacterium Xanthomonas campestris, PcrK, as a bacterial receptor that specifically detects the plant cytokinin 2-isopentenyladenine (2iP). 2iP binds to the extracytoplasmic region of PcrK to decrease its autokinase activity...
December 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/29210676/enrichment-and-application-of-nitrifying-activated-sludge-in-membrane-bioreactors
#17
Xiaolin Sheng, Rui Liu, Lujun Chen, Zihua Yin, Jianfeng Zhu
In this study, nitrifying bacteria were enriched in a membrane bioreactor (MBR, R1) and their bioaugmentation effectiveness was evaluated in another two MBRs (R2 and R3). Nitrifying activated sludge (NAS) with high nitrification activity of up to 3,000 mg-N/(L·d)-1 was successfully enriched in R1. The results showed that chemical oxygen demand concentration of 100-200 mg/L had no negative effect on NAS enrichment but reduced the ratio of bacterial nitrifiers. Moreover, the cell concentration of nitrifying bacteria in NAS, which was 3...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29209964/probing-the-toxicity-mechanism-of-multiwalled-carbon-nanotubes-on-bacteria
#18
Maria R Hartono, Ariel Kushmaro, Xiaodong Chen, Robert S Marks
Carbon nanotubes (CNTs) have emerged recently as superior adsorbent materials for the removal of recalcitrant pollutants. The potential of combining the sorption capability of CNTs with bacterial degradation for pollutant removal, however, necessitates further investigation of the mechanisms of CNTs' toxicity towards bacterial cells. In this study, we used a panel of stress-responsive recombinant Escherichia coli bioluminescence bacterial strains to explore the possible mechanisms of toxicity of multiwalled carbon nanotubes (MWCNTs)...
December 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29209784/acaxl2-and-acmst1-regulate-arthrospore-development-and-stress-resistance-in-the-cephalosporin-c-producer-acremonium-chrysogenum
#19
Janina Kluge, Ulrich Kück
The filamentous fungus Acremonium chrysogenum is the primordial producer of the β-lactam antibiotic cephalosporin C. This antibiotic is of major biotechnological and medical relevance because of its antibacterial activity against Gram-positive and Gram-negative bacteria. Antibiotic production during the lag phase of fermentation is often accompanied by a typical morphological feature of A. chrysogenum, the fragmentation of the mycelium into arthrospores. Here, we sought to identify factors that regulate the hyphal septation process and present the first comparative functional characterization of the type I integral plasma membrane protein Axl2 (axial budding pattern protein 2), a central component of the bud site selection system (BSSS) and Mst1 (mammalian Sterile20-like kinase), a septation initiation network (SIN)-associated germinal center kinase (GCK)...
December 5, 2017: Current Genetics
https://www.readbyqxmd.com/read/29209295/extractive-fermentation-of-lactic-acid-in-lactic-acid-bacteria-cultivation-a-review
#20
REVIEW
Majdiah Othman, Arbakariya B Ariff, Leonardo Rios-Solis, Murni Halim
Lactic acid bacteria are industrially important microorganisms recognized for their fermentative ability mostly in their probiotic benefits as well as lactic acid production for various applications. Nevertheless, lactic acid fermentation often suffers end-product inhibition which decreases the cell growth rate. The inhibition of lactic acid is due to the solubility of the undissociated lactic acid within the cytoplasmic membrane and insolubility of dissociated lactate, which causes acidification of cytoplasm and failure of proton motive forces...
2017: Frontiers in Microbiology
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