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https://www.readbyqxmd.com/read/27776270/validity-and-sensitivity-of-the-longitudinal-asymmetry-index-to-detect-gait-asymmetry-using-microsoft-kinect-data
#1
E Auvinet, F Multon, V Manning, J Meunier, J P Cobb
Gait asymmetry information is a key point in disease screening and follow-up. Constant Relative Phase (CRP) has been used to quantify within-stride asymmetry index, which requires noise-free and accurate motion capture, which is difficult to obtain in clinical settings. This study explores a new index, the Longitudinal Asymmetry Index (ILong) which is derived using data from a low-cost depth camera (Kinect). ILong is based on depth images averaged over several gait cycles, rather than derived joint positions or angles...
August 24, 2016: Gait & Posture
https://www.readbyqxmd.com/read/27609092/in-vivo-bioluminescence-imaging-for-leptomeningeal-dissemination-of-medulloblastoma-in-mouse-models
#2
Seung Ah Choi, Pil Ae Kwak, Seung-Ki Kim, Sung-Hye Park, Ji Yeoun Lee, Kyu-Chang Wang, Hyun Jeong Oh, Kyuwan Kim, Dong Soo Lee, Do Won Hwang, Ji Hoon Phi
BACKGROUND: The primary cause of treatment failure in medulloblastomas (MB) is the development of leptomeningeal dissemination (seeding). For translational research on MB seeding, one of the major challenges is the development of reliable experimental models that simulate the seeding and growth characteristics of MBs. To overcome this obstacle, we improved an experimental mouse model by intracisternal inoculation of human MB cells and monitoring with in vivo live images. METHODS: Human MB cells (UW426, D283 and MED8A) were transfected with a firefly luciferase gene and a Thy1...
2016: BMC Cancer
https://www.readbyqxmd.com/read/26931337/complex-interfaces-in-phase-change-contrast-agents
#3
Sabrina Capece, Fabio Domenici, Francesco Brasili, Letizia Oddo, Barbara Cerroni, Angelico Bedini, Federico Bordi, Ester Chiessi, Gaio Paradossi
In this paper we report on the study of the interface of hybrid shell droplets encapsulating decafluoropentane (DFP), which exhibit interesting potentialities for ultrasound (US) imaging. The fabrication of the droplets is based on the deposition of a dextran methacrylate layer onto the surface of surfactants. The droplets have been stabilized against coalescence by UV curing, introducing crosslinks in the polymer layer and transforming the shell into an elastomeric membrane with a thickness of about 300 nm with viscoelastic behaviour...
March 28, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/26449846/a-rapid-and-simple-method-for-efficient-capture-and-accurate-discrimination-of-circulating-tumor-cells-using-aptamer-conjugated-magnetic-beads-and-surface-enhanced-raman-scattering-imaging
#4
Changlong Sun, Ren Zhang, Mingxia Gao, Xiangmin Zhang
The efficient isolation and the accurate phenotype discrimination of circulating tumor cells (CTCs) are expected to provide much valuable information for the understanding of tumor metastasis and to play an important role in personalized treatment of cancer patients in the future. In this study, we developed a novel, rapid, and simple method for efficient capture and accurate identification of CTCs using aptamer conjugated magnetic beads and surface-enhanced Raman scattering (SERS) imaging technique. Using aptamer conjugated magnetic beads, rare target cancer cells can be captured efficiently from buffer and whole blood sample with capture efficiency of 73 % and 55 %, respectively...
November 2015: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/24763818/rapid-fluorescent-detection-of-escherichia-coli-k88-based-on-dna-aptamer-library-as-direct-and-specific-reporter-combined-with-immuno-magnetic-separation
#5
Zhihui Peng, Min Ling, Yi Ning, Le Deng
Nucleic acid aptamers have long demonstrated the capacity to bind cells with high affinity so that they have been utilized to diagnose various important pathogens. In this study, a DNA aptamer library was on initial efforts developed to act as a specific reporter for rapid detection of enter toxigenic Escherichia coli (ETEC) K88 combined with immuno-magnetic separation (IMS). During a Whole-cell Systematic Evolution of Ligands by Exponential Enrichment (CELL-SELEX) procedure, the last selection pool against ETEC K88, which is named "DNA aptamer library" here, was selected and subsequently identified by flow cytometric analysis and confocal imaging...
July 2014: Journal of Fluorescence
https://www.readbyqxmd.com/read/9174926/development-of-an-immunomagnetic-assay-system-for-rapid-detection-of-bacteria-and-leukocytes-in-body-fluids
#6
J G Bruno, H Yu, J P Kilian, A A Moore
Immunomagnetic (IM) separation and concentration of specific target ligands or particles, such as bacteria or leukocytes, from complex mixtures, such as bone marrow, blood and other body fluids, is now a widely accepted technique. IM methodologies require high affinity antibodies or other receptors, but are potentially as effective as density gradient separations. Thus, a computer-controlled first-generation immunomagnetic assay system (IMAS) biodetector is being developed for clinical diagnostics. This system is fully automated and affords the advantage of rapid flow-through capture of all types of magnetic beads (MBs) and obviates operator contact with body fluid samples during the collection and analysis phases...
September 1996: Journal of Molecular Recognition: JMR
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