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https://www.readbyqxmd.com/read/28743116/the-role-of-tumoral-foxp3-on-cell-proliferation-migration-and-invasion-in-gastric-cancer
#1
Lu Zhang, Jianghao Xu, Xuan Zhang, Yi Zhang, Lu Wang, Xiaoxu Huang, Zekuan Xu
BACKGROUND/AIMS: There is little published data on the role of FOXP3 in gastric cancer. METHODS: FOXP3 expression and localization in gastric cancer tissues and cells were examined by immunohistochemistry, RT-PCR, flow cytometry, western blot, and laser confocal microscopy. CCK8, plate clone, wound healing, and transwell insert assays were performed for gastric cancer cells. Potential molecules and signaling pathways were screened using high-throughput transcriptome sequencing...
July 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743091/performance-comparison-of-two-herbal-and-industrial-medicines-using-nanoparticles-with-a-starch-cellulose-shell-and-alginate-core-for-drug-delivery-in-vitro-studies
#2
Akbar Esmaeili, Sahar Behzadi
In this study, the performance of two kinds of industrial and herbal drugs encapsulated in nanoparticles with a shell of starch and cellulose and an alginate core were examined as a new technique for nanoparticle drug delivery. The test method involved creating a suspension of starch and alginate, which was then dried, mixed with cellulose, and heated to form core-shell nanoparticles. The industrial drug calcitonin and an extract of the herb Amaranthus retroflexcus L. were added separately and in combination to the nanoparticles, and the performance of each configuration was evaluated...
July 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28743086/effect-of-cinnamon-cinnamomum-verum-bark-essential-oil-on-the-halitosis-associated-bacterium-solobacterium-moorei-and-in-vitro-cytotoxicity
#3
Geneviève LeBel, Bruno Haas, Andrée-Ann Adam, Marie-Pier Veilleux, Amel Ben Lagha, Daniel Grenier
OBJECTIVES: Halitosis, also known as bad breath or oral malodour, is a condition affecting a large proportion of the population. Solobacterium moorei is a Gram-positive anaerobic bacterium that has been specifically associated with halitosis. In this study, we investigated the effects of essential oils, more particularly cinnamon bark oil, on growth, biofilm formation, eradication and killing, as well as hydrogen sulfide (H2S) production by S. moorei. METHODS: A broth microdilution assay was used to determine the antibacterial activity of essential oils...
July 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28743029/thermoresponsive-nanoparticle-agglomeration-aggregation-in-salt-solutions-dependence-on-graft-density
#4
Thaddeus W Vasicek, Samir V Jenkins, Leticia Vaz, Jingyi Chen, Julie A Stenken
Gold nanoparticles with a graft density of 0.09, 0.30 and 0.40chains/nm(2) of poly(N-isopropylacrylamide) were reproducibly synthesized by varying the ratio of disulfide terminated poly(N-isopropylacrylamide) to gold nanoparticle. The polymer coated nanoparticles were stable at room temperature in 50mM NaCl, yet agglomerated at 37°C. Previous studies have observed conflicting results as to the reversibility of this agglomeration. Particle agglomeration with three different graft densities was studied in 50mM NaCl by measurements of their localized surface plasmon resonance and hydrodynamic diameter, and imaging with electron microscopy...
July 17, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28743025/rna-polymerase-i-and-iii-similar-yet-unique
#5
REVIEW
Heena Khatter, Matthias K Vorländer, Christoph W Müller
The majority of non-protein-coding RNAs present in eukaryotic cells comprises rRNAs, tRNAs and U6 snRNA that are involved in protein biosynthesis and are synthesized by DNA-dependent-RNA polymerase I and III. The transcription cycle (initiation, elongation and termination) has similar principles in all three nuclear RNA polymerases with specific features that are reflected back in their structures. Recently, owing to the 'resolution revolution' in electron cryo-microscopy, there has been a significant advancement in the understanding of these molecular machines...
July 22, 2017: Current Opinion in Structural Biology
https://www.readbyqxmd.com/read/28742972/stimuli-responsive-directional-vesicular-assembly-with-tunable-surface-functionality-and-impact-on-enzyme-inhibition
#6
Amrita Sikder, Debes Ray, Vinod Kumar Aswal, Suhrit Ghosh
The article describes self-assembly of a series of unsymmetrical bola-shape π-amphiphiles (NDI-1, NDI-1a, NDI-2, NDI-3 and NDI-4) consisting of a hydrophobic naphthalene-diimide (NDI) chromophore attached with a non-ionic hydrophilic wedge and an anionic head group in the two opposite arms of the central NDI. By design only a single hydrazide group is linked either on the ionic or non-ionic arm of the NDI. NDI-1 and NDI-1a are regioisomers differing only in the location of the hydrazide group, placed in the non-ionic or ionic arm, respectively...
July 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28742922/intracellular-imaging-of-docosanol-in-living-cells-by-coherent-anti-stokes-raman-scattering-microscopy
#7
Sixian You, Yuan Liu, Zane Arp, Youbo Zhao, Eric J Chaney, Marina Marjanovic, Stephen A Boppart
No abstract text is available yet for this article.
July 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28742634/fingolimod-fty-720-is-capable-of-reversing-tumor-necrosis-factor-induced-decreases-in-cochlear-blood-flow
#8
Mattis Bertlich, Friedrich Ihler, Bernhard G Weiss, Saskia Freytag, Mark Jakob, Michael Strupp, Hannah Pellkofer, Martin Canis
HYPOTHESIS: The potential of Fingolimod (FTY-720), a sphingosine-1-phosphate analogue, to revoke the changes in cochlear blood flow induced by tumor necrosis factor (TNF) was investigated. BACKGROUND: Impairment of cochlear blood flow has often been considered as the common final pathway of various inner ear pathologies. TNF, an ubiquitous cytokine, plays a major role in these pathologies, reducing cochlear blood flow via sphingosine-1-phosphate-signaling. METHODS: Fifteen Dunkin-Hartley guinea pigs were randomly assigned to one of three groups (placebo/placebo, TNF/placebo, TNF/FTY-720)...
July 24, 2017: Otology & Neurotology
https://www.readbyqxmd.com/read/28742332/modulation-of-lipid-digestion-profiles-using-filled-egg-white-protein-microgels
#9
Luping Gu, Yujie Su, Zipei Zhang, Bingjing Zheng, Ruojie Zhang, David J McClements, Yan-Jun Yang
Colloidal delivery systems are required to encapsulate, protect, and release active food ingredients, such as vitamins, nutraceuticals, and minerals. In this study, lipid droplets were encapsulated within biopolymer microgels fabricated from egg white proteins an injection-gelation process. Confocal fluorescence microscopy indicated that lipid droplets were dispersed within a network of crosslinked proteins within the microgels. The properties of the lipid-loaded microgels were compared to those of simple oil-in-water emulsions stabilized by egg white protein...
July 25, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28742320/nanoscale-probing-of-elastic-electronic-response-to-vacancy-motion-in-nio-nanocrystals
#10
Fran Kurnia, Jeffrey Cheung, Xuan Cheng, Jivika Sullaphen, Sergei V Kalinin, Nagarajan Valanoor, Rama K Vasudevan
Measuring the diffusion of ions and vacancies at nanometer length scales is crucial to understanding fundamental mechanisms driving technologies as diverse as batteries, fuel cells and memristors; yet such measurements remains extremely challenging. Here, we employ a multi-modal scanning probe microscopy (SPM) technique to explore the interplay between electronic, elastic and ionic processes via first order reversal curve I-V measurements in conjunction with electrochemical strain microscopy (ESM). The technique is employed to investigate the diffusion of oxygen vacancies in model epitaxial nickel oxide (NiO) nanocrystals with resistive switching characteristics...
July 25, 2017: ACS Nano
https://www.readbyqxmd.com/read/28742085/clinically-severe-cacna1a-alleles-affect-synaptic-function-and-neurodegeneration-differentially
#11
Xi Luo, Jill A Rosenfeld, Shinya Yamamoto, Tamar Harel, Zhongyuan Zuo, Melissa Hall, Klaas Wierenga, Matthew T Pastore, Dennis Bartholomew, Mauricio R Delgado, Joshua Rotenberg, Richard Alan Lewis, Lisa Emrick, Carlos A Bacino, Mohammad K Eldomery, Zeynep Coban Akdemir, Fan Xia, Yaping Yang, Seema R Lalani, Timothy Lotze, James R Lupski, Brendan Lee, Hugo J Bellen, Michael F Wangler
Dominant mutations in CACNA1A, encoding the α-1A subunit of the neuronal P/Q type voltage-dependent Ca2+ channel, can cause diverse neurological phenotypes. Rare cases of markedly severe early onset developmental delay and congenital ataxia can be due to de novo CACNA1A missense alleles, with variants affecting the S4 transmembrane segments of the channel, some of which are reported to be loss-of-function. Exome sequencing in five individuals with severe early onset ataxia identified one novel variant (p.R1673P), in a girl with global developmental delay and progressive cerebellar atrophy, and a recurrent, de novo p...
July 24, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28741935/super-photostable-phosphole-based-dye-for-multiple-acquisition-stimulated-emission-depletion-imaging
#12
Chenguang Wang, Masayasu Taki, Yoshikatsu Sato, Aiko Fukazawa, Tetsuya Higashiyama, Shigehiro Yamaguchi
As stimulated emission depletion (STED) microscopy can provide structural details of cells with an optical resolution beyond the diffraction limit, it has become an indispensable tool in cell biology. However, the intense STED laser beam usually causes rapid photobleaching of the employed fluorescent dyes, which significantly limits the utility of STED microscopy from a practical perspective. Herein we report a new design of super-photostable dye, PhoxBright 430 (PB430), comprising a fully ring-fused π-conjugated skeleton with an electron-accepting phosphole P-oxide unit...
July 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28741902/corneal-confocal-sub-basal-nerve-plexus-evaluation-a-review
#13
REVIEW
Joanna Kokot, Adam Wylęgała, Bogumił Wowra, Łukasz Wójcik, Dariusz Dobrowolski, Edward Wylęgała
The aim of this study was to review the most recent data about corneal sub-basal nerve plexus (SNP) evaluated with the use of corneal confocal microscopy (CCM). For this purpose, an electronic search was conducted based on PubMed and Google Scholar and Web of Science databases from 2008 up to the end of 2016. Ninety-eight articles in English were cited, as well as abstracts in other languages, concerning the morphology and function of corneal SNP in various diseases. Changes in corneal SNP as a result of local treatment were also introduced...
July 25, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28741862/in-vivo-comparison-of-jellyfish-and-bovine-collagen-sponges-as-prototype-medical-devices
#14
Jonathan P Widdowson, Alex J Picton, Valerie Vince, Chris J Wright, Andrew Mearns-Spragg
Jellyfish have emerged as a source of next generation collagen that is an attractive alternative to existing sources, such as bovine and porcine, due to a plentiful supply and providing a safer source through lack of bovine spongiform encephalopathy (BSE) transmission risk and potential viral vectors, both of which could be transmitted to humans. Here we compare collagen implantable sponges derived for the first time from the Rhizostoma pulmo jellyfish. A further novelty for the research was that there was a comparison for sponges that were either uncrosslinked or crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and an assessment on how this affected resorption, as well as their biocompatibility compared to bovine type I collagen sponges...
July 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28741861/parvularia-atlantis-gen-et-sp-nov-a-nucleariid-filose-amoeba-holomycota-opisthokonta
#15
David López-Escardó, Purificación López-García, David Moreira, Iñaki Ruiz-Trillo, Guifré Torruella
The opisthokonts constitute a eukaryotic supergroup divided into two main clades: the holozoans, which include animals and their unicellular relatives, and the holomycotans, which include fungi, opisthosporidians and nucleariids. Nucleariids are phagotrophic filose amoebae that phenotypically resemble more their distant holozoan cousins than their holomycotan phylogenetic relatives. Despite their evolutionary interest, the diversity and internal phylogenetic relationships within the nucleariids remain poorly studied...
July 25, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28741840/stepwise-growth-of-fullerene-nanoparticles-through-guest-exchange-of-%C3%AE-cyclodextrin-complexes-in-water
#16
Kouta Sugikawa, Kentaro Kozawa, Masafumi Ueda, Atsushi Ikeda
Stepwise growth of fullerene nanoparticles (nCx; x=60 or 70) was performed through guest exchange of a γ-cyclodextrin (γ-CD) complex. Fullerenes bicapped by γ-CD were mixed with presynthesized nCx in the presence of polyethylene glycol (PEG) in water. Fullerenes expelled from the γ-CD hosts by PEG were piled on the original nCx, resulting in the growth of nCx. This process could be repeated and the size of nCx increased according to the number of growth steps. The growth of fullerene nanoparticles was confirmed by UV/Vis absorption spectroscopy, dynamic light scattering measurements, and transmission electron microscopy observations...
July 25, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28741793/telomerase-enzyme-deficiency-promotes-metabolic-dysfunction-in-murine-hepatocytes-upon-dietary-stress
#17
Raquel M Alves-Paiva, Sachiko Kajigaya, Xingmin Feng, Jichun Chen, Marie Desierto, Susan Wong, Danielle M Townsley, Flávia S Donaires, Adeline Bertola, Bin Gao, Neal S Young, Rodrigo T Calado
Short telomeres and genetic telomerase defects are risk factors for some human liver diseases, ranging from non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) to cirrhosis. In murine models, telomere dysfunction has been shown to metabolically compromise hematopoietic cells, liver, and heart via the activation of the p53-PGC axis METHODS: Tert- and Terc-deficient mice were challenged with liquid high fat diet (HFD). Liver metabolic contents were analyzed by CE-TOFMS and liver fat content was confirmed by confocal and electronic microscopy RESULTS: Tert-deficient but not Terc-deficient mice develop hepatocyte injury and frank steatosis when challenged with liquid HFD...
July 25, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28741727/placental-pericytes-and-cytomegalovirus-infectivity-implications-for-hcmv-placental-pathology-and-congenital-disease
#18
David M Aronoff, Hernan Correa, Lisa M Rogers, Ravit Arav-Boger, Donald J Alcendor
PROBLEM: Placental pericytes are essential for placental microvascular function, stability, and integrity. Mechanisms of human cytomegalovirus (HCMV) pathogenesis incorporating placental pericytes are unknown. METHOD OF STUDY: HCMV-infected placental tissue was stained by dual-labeled immunohistochemistry. Primary placental pericytes, cytotrophoblasts, and villous fibroblasts were exposed to HCMV; and infectivity was analyzed by microscopy and immunofluorescence...
July 25, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28741699/the-flagellar-apparatus-of-the-glaucophyte-cyanophora-cuspidata
#19
Aaron A Heiss, Alaric W Heiss, Kaleigh Lukacs, Eunsoo Kim
Glaucophytes are a kingdom-scale lineage of unicellular algae with uniquely underived plastids. The genus Cyanophora is of particular interest because it is the only glaucophyte that is a flagellate throughout its life cycle, making its morphology more directly comparable than other glaucophytes to other eukaryote flagellates. The ultrastructure of Cyanophora has already been studied, primarily in the 1960s and 1970s. However, the usefulness of that work has been undermined by its own limitations, subsequent misinterpretations, and a recent taxonomic revision of the genus...
July 25, 2017: Journal of Phycology
https://www.readbyqxmd.com/read/28741594/polar-phase-transitions-in-heteroepitaxial-stabilized-la-sub-0-5-sub-y-sub-0-5-sub-alo-sub-3-sub-thin-films
#20
Shenghua Liu, Chunfeng Zhang, Mengya Zhu, Qian He, Jak Chakhalian, Xiaoran Liu, Albina Borisevich, Xiaoyong Wang, Min Xiao
We report on the fabrication of epitaxial La0.5Y0.5AlO3 ultrathin films on (001) LaAlO3 substrates. Structural characterizations by scanning transmission electron microscopy and X-ray diffraction confirm the high quality of the film with AlO6 octahedral tilt pattern. Unlike either of the nonpolar parent compound, LaAlO3 and YAlO3, second harmonic generation measurements on the thin films suggest a nonpolar - polar phase transition at Tc near 500 K, and a polar - polar phase transition at Ta near 160 K. By fitting the angular dependence of the second harmonic intensities, we further propose that the two polar structures can be assigned to the Pmc21 and Pmn21 space group, while the high temperature nonpolar structure belongs to the Pbnm space group...
July 25, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
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