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Microscopy

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https://www.readbyqxmd.com/read/28228001/fabrication-of-progesterone-loaded-nanofibers-for-the-drug-delivery-applications-in-bovine
#1
Chitra Karuppannan, Mehnath Sivaraj, J Ganesh Kumar, Rangasamy Seerangan, S Balasubramanian, Dhinakar Raj Gopal
Progesterone is a potent drug for synchronization of the estrus and ovulation cycles in bovine. At present, the estrus cycle of bovine is controlled by the insertion of progesterone-embedded silicone bands. The disadvantage of nondegradable polymer inserts is to require for disposal of these bands after their use. The study currently focuses on preparation of biodegradable progesterone-incorporated nanofiber for estrus synchronization. Three different concentrations (1.2, 1.9, and 2.5 g) of progesterone-impregnated nanofibers were fabricated using electrospinning...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28227986/automated-neurovascular-tracing-and-analysis-of-the-knife-edge-scanning-microscope-rat-nissl-data-set-using-a-computing-cluster
#2
Sungjun Lim, Michael R Nowak, Yoonsuck Choe, Sungjun Lim, Michael R Nowak, Yoonsuck Choe, Michael R Nowak, Sungjun Lim, Yoonsuck Choe
We present a novel, parallelizable algorithm capable of automatically reconstructing and calculating anatomical statistics of cerebral vascular networks embedded in large volumes of Rat Nissl-stained data. In this paper, we report the results of our method using Rattus somatosensory cortical data acquired using Knife-Edge Scanning Microscopy. Our algorithm performs the reconstruction task with averaged precision, recall, and F2-score of 0.978, 0.892, and 0.902 respectively. Calculated anatomical statistics show some conformance to values previously reported...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227867/knife-edge-scanning-microscopy-for-in-silico-study-of-cerebral-blood-flow-from-biological-imaging-data-to-flow-simulations
#3
Michael R Nowak, Alexander Lozovskiy, Dimitri Dobroskok, Yoonsuck Choe, Michael R Nowak, Alexander Lozovskiy, Dimitri Dobroskok, Yoonsuck Choe, Dimitri Dobroskok, Michael R Nowak, Alexander Lozovskiy, Yoonsuck Choe
Knife-edge scanning microscopy provides the capability to image whole-brain cerebral microvasculature of small organisms, such as mice, at sub-micron resolution, providing a feasible foundation for the reconstruction of circulatory pathways from the systemic to cellular scale. In this paper, we illustrate the feasibility of using this data to model cerebral blood flow using numerical simulations. Starting with a small vascular element in microcirculation of interest, we present its segmentation from the imaging-data volume, construction of its triangular surface mesh, assembly of its tetrahedral volumetric mesh from the surface, and then conclude with Stokes flow simulation of plasma through the microvascular vessel...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227866/modelling-nutrient-transfer-based-on-3d-imaging-of-the-human-placental-microstructure
#4
S Perazzolo, R M Lewis, B G Sengers, S Perazzolo, R M Lewis, B G Sengers, B G Sengers, R M Lewis, S Perazzolo
Impaired transfer of nutrients from mother to fetus can affect pregnancy outcomes. The placenta has a complex microstructure, including the maternal intervillous space and fetal capillaries. Previous computational models of placental transfer either assumed a simplified idealized local geometry or were based on 2D imaging. In this study, we present a novel 3D computational model to assess the placental transfer of nutrients at the microscale in interaction with the maternal flow environment. A stack of confocal microscopy images of the placental terminal villi was collected and reconstructed...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227853/fast-submicrometer-scale-imaging-of-whole-zebrafish-using-the-knife-edge-scanning-microscope
#5
Daniel E Miller, Raj S Shah, Wencong Zhang, Jaewook Yoo, Jaerock Kwon, David Mayerich, John Keyser, Louise C Abbott, Yoonsuck Choe, Daniel E Miller, Raj S Shah, Wencong Zhang, Jaewook Yoo, Jaerock Kwon, David Mayerich, John Keyser, Louise C Abbott, Yoonsuck Choe, Jaewook Yoo, Louise C Abbott, Wencong Zhang, John Keyser, Raj S Shah, Daniel E Miller, Jaerock Kwon, David Mayerich, Yoonsuck Choe
Advances in high-resolution 3D microscopy have enabled the investigation of subcellular microstructures in biological specimen. For a full understanding of the organism's structure and function, it is mandatory to obtain data from the whole animal, not just parts of it. In this paper, we present our work with the Knife-Edge Scanning Microscope (KESM) for imaging a Nissl-stained whole zebrafish larva. KESM combines a diamond microtome and line-scan imaging for simultaneous sectioning and imaging in 3D. We show that using the KESM, a zebrafish, less than 3 mm long and diameter less than 500 μm, can be imaged within 1 hour at a resolution of 0...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227441/novel-ti-nb-alloys-with-improved-wear-resistance-for-biomedical-implant-application
#6
Praveen Chapala, Swati Ghosh Acharyya, S M Shariff, Ganesh Naik, Praveen Chapala, Swati Ghosh Acharyya, S M Shariff, Ganesh Naik, Ganesh Naik, S M Shariff, Praveen Chapala, Swati Ghosh Acharyya
The present study involved fabrication of titanium niobium based alloys having elastic modulus close to that of human bone and subsequently determining the wear resistance of these alloys for biomedical application. In the present study three beta stabilized titanium niobium alloys having composition Ti-24Nb-4Zr-8Sn, Ti-35Nb-4Sn and Ti-29Nb-13Ta-7Zr were made by vacuum arc melting. The alloys were then aged at 1000 C for 6 hours in an argon atmosphere. Detailed microstructural characterization and phase identification was carried out using scanning electron microscopy and x-ray diffraction respectively...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227393/an-integrated-neuroprotective-intervention-for-brain-ischemia-validated-by-ecog-fpam
#7
Yu-Hang Liu, Lun-De Liao, Su Jing Chan, Aishwarya Bandla, Nitish V Thakor, Yu-Hang Liu, Lun-De Liao, Su Jing Chan, Aishwarya Bandla, Nitish V Thakor, Yu-Hang Liu, Aishwarya Bandla, Nitish V Thakor, Su Jing Chan, Lun De Liao
Brain ischemia is a neurological deficit caused by a reduction in the blood supply to tissue, and one of the leading causes of disability in the world. Currently, the most well-known therapeutic agent for ischemia recovery is recombinant tissue plasminogen activator (rtPA), but it is viable for only a small portion (approximately 3.6%) of ischemic patients and may cause side effects such as tissue damage. Thus, introducing a new therapeutic concept for ischemia, we proposed an integrated intervention combining global and focal stimulations in this article...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227388/learning-to-distinguish-cerebral-vasculature-data-from-mechanical-chatter-in-india-ink-images-acquired-using-knife-edge-scanning-microscopy
#8
Michael R Nowak, Yoonsuck Choe, Michael R Nowak, Yoonsuck Choe, Michael R Nowak, Yoonsuck Choe
We introduce a simple, yet effective, procedure for accurate classification of connected components embedded in biological images. In our method, a training set is generated from user-delineated features of manually-labeled examples; we subsequently train a classifier using the resultant training set. The overall process is described using imaging data acquired from an India-ink perfused C57BL/6J mouse brain using Knife Edge Scanning Microscopy. We illustrate the procedure through segmentation of cerebral vasculature structures from mechanical noise using trained classifiers...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227387/detecting-cell-division-of-pseudomonas-aeruginosa-bacteria-from-bright-field-microscopy-images-with-hidden-conditional-random-fields
#9
Lee-Ling S Ong, Xinghua Zhang, Binu Kundukad, Justin Dauwels, Patrick Doyle, H Harry Asada, Lee-Ling S Ong, Xinghua Zhang, Binu Kundukad, Justin Dauwels, Patrick Doyle, H Harry Asada, Binu Kundukad, Lee-Ling S Ong, Xinghua Zhang, Justin Dauwels, Patrick Doyle, H Harry Asada
An approach to automatically detect bacteria division with temporal models is presented. To understand how bacteria migrate and proliferate to form complex multicellular behaviours such as biofilms, it is desirable to track individual bacteria and detect cell division events. Unlike eukaryotic cells, prokaryotic cells such as bacteria lack distinctive features, causing bacteria division difficult to detect in a single image frame. Furthermore, bacteria may detach, migrate close to other bacteria and may orientate themselves at an angle to the horizontal plane...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227386/image-classification-of-unlabeled-malaria-parasites-in-red-blood-cells
#10
Zheng Zhang, L L Sharon Ong, Kong Fang, Athul Matthew, Justin Dauwels, Ming Dao, Harry Asada, Zheng Zhang, L L Sharon Ong, Kong Fang, Athul Matthew, Justin Dauwels, Ming Dao, Harry Asada, Justin Dauwels, Zheng Zhang, Kong Fang, Ming Dao, Harry Asada
This paper presents a method to detect unlabeled malaria parasites in red blood cells. The current "gold standard" for malaria diagnosis is microscopic examination of thick blood smear, a time consuming process requiring extensive training. Our goal is to develop an automate process to identify malaria infected red blood cells. Major issues in automated analysis of microscopy images of unstained blood smears include overlapping cells and oddly shaped cells. Our approach creates robust templates to detect infected and uninfected red cells...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227385/statistical-characterization-of-ensembles-of-symmetric-virus-particles-3-d-stochastic-signal-reconstruction-from-electron-microscope-images
#11
Nan Xu, Peter C Doerschuk, Nan Xu, Peter C Doerschuk, Nan Xu, Peter C Doerschuk
Stochastic models of nano-biomachines have been studied by 3-D reconstruction from cryo electron microscopy images in recent years. The image data is the projection of many heterogeneous instances of the object under study (e.g., a virus). Initial reconstruction algorithms require different instances of the object, while still heterogeneous, to have the same symmetry. This paper presents a maximum likelihood reconstruction approach which allows each object to lack symmetry while constraining the statistics of the ensemble of objects to have symmetry...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227372/multimodal-embryonic-imaging-using-optical-coherence-tomography-selective-plane-illumination-microscopy-and-optical-projection-tomography
#12
M Singh, C Wu, D Mayerich, M E Dickinson, I V Larina, K V Larin, M Singh, C Wu, D Mayerich, M E Dickinson, I V Larina, K V Larin, I V Larina, M Singh, K V Larin, D Mayerich, M E Dickinson, C Wu
The murine model is commonly utilized for studying developmental diseases. Different optical techniques have been developed to image mouse embryos, but each has its own set of limitations and restrictions. In this study, we compare the performance of the well-established technique of optical coherence tomography (OCT) to the relatively new methods of selective plane illumination microscopy (SPIM) and optical projection tomography (OPT) to assess murine embryonic development. OCT can provide label free high resolution images of the mouse embryo, but suffers from light attenuation that limits visualization of deeper structures...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227370/detection-and-localization-of-mouse-induced-pluripotent-stem-cell-formation-using-time-lapse-fluorescence-microscopy-images
#13
Yuan-Hsiang Chang, Hideo Yokota, Kuniya Abe, Jung Hsien Liu, Ming-Dar Tsai, Yuan-Hsiang Chang, Hideo Yokota, Kuniya Abe, Jung Hsien Liu, Ming-Dar Tsai, Yuan-Hsiang Chang, Kuniya Abe, Ming-Dar Tsai, Hideo Yokota, Jung Hsien Liu
We present an automated method for detection and localization of mouse Induced pluripotent stem (iPS) cells formation by using the fluorescence microscopy images. The differentiated cells that possibly undergo reprogramming to iPS cells can be detected by this method for screening reagents or culture conditions in iPS induction. Our method includes image preprocessing to enhance the fluorescence microscopy images, followed by cell detection to detect isolated fluorescent (reprogramming or reprogrammed iPS) cells...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227365/integrated-quantification-based-on-confocal-imaging-cell-crowding-modulates-heterogeneity-in-gpcr-mediated-calcium-oscillation
#14
Jophin G Joseph, S Nanda, S Vennamalla, G Bhure, Ranjana Singh, Soumya Jana, Lopamudra Giri, Jophin G Joseph, S Nanda, S Vennamalla, G Bhure, Ranjana Singh, Soumya Jana, Lopamudra Giri, Soumya Jana, Lopamudra Giri, Jophin G Joseph, S Nanda, Ranjana Singh, G Bhure, S Vennamalla
Results from single cell imaging, facilitated by high resolution microscopy, have demonstrated cell-to-cell variability within the same cell population in contexts ranging from cell growth to cell migration. Recent studies suggest that such variability conveys important information about diseased states. However, manual analysis and interpretation of heterogeneous calcium oscillation based on time-lapsed images, as practiced today, is tedious, and essentially infeasible for large datasets. As a practical alternative, we present an integrated platform that includes calcium imaging using confocal microscope, algorithmic cell segmentation, and statistical analysis...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227364/a-method-to-quantify-co-localization-in-biological-images
#15
Shiwen Zhu, Roy E Welsch, Paul T Matsudaira, Shiwen Zhu, Roy E Welsch, Paul T Matsudaira, Roy E Welsch, Paul T Matsudaira, Shiwen Zhu
Quantitative co-localization analysis with fluorescent microscopy is a common approach to assess the spatial co-ordination of molecules and thus to understand their functions in biological processes. However, the co-localization analysis results might not be consistent due to various imaging conditions and different quantification methods used. We propose a novel method to separate a co-localization event into two aspects: co-occurrence and intensity correlation, which are usually combined as one parameter in other quantitative co-localization analyses...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227363/3d-gabor-wavelet-based-vessel-filtering-of-photoacoustic-images
#16
Israr Ul Haq, Ryo Nagoaka, Takahiro Makino, Takuya Tabata, Yoshifumi Saijo, Israr Ul Haq, Ryo Nagoaka, Takahiro Makino, Takuya Tabata, Yoshifumi Saijo, Ryo Nagoaka, Yoshifumi Saijo, Takahiro Makino, Takuya Tabata
Filtering and segmentation of vasculature is an important issue in medical imaging. The visualization of vasculature is crucial for the early diagnosis and therapy in numerous medical applications. This paper investigates the use of Gabor wavelet to enhance the effect of vasculature while eliminating the noise due to size, sensitivity and aperture of the detector in 3D Optical Resolution Photoacoustic Microscopy (OR-PAM). A detailed multi-scale analysis of wavelet filtering and Hessian based method is analyzed for extracting vessels of different sizes since the blood vessels usually vary with in a range of radii...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28227124/noninvasive-surface-damage-assessment-of-bovine-articular-cartilage-explants-by-reflected-polarized-light-microscopy
#17
Ruby N Huynh, Christopher B Raub, Ruby N Huynh, Christopher B Raub, Ruby N Huynh, Christopher B Raub
Articular surface damage is a hallmark of cartilage degeneration. Noninvasive assessment of cartilage microstructural alterations has potential clinical value. In this study, we use bovine patellofemoral articular cartilage explants treated with mechanical scraping and collagenase to create cartilage surface disruption, and use polarized reflectance microscopy to quantify alterations to surface and sub-surface microstructure. Reflected polarized signal was sensitive to mild damage to the cartilage surface, and highlighted disruptive alterations...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226980/gastric-mucosa-injury-quantification-in-an-ischemia-reperfusion-experimental-model
#18
E Pena-Mercado, M Garcia-Lorenzana, E Arechaga, C H Gonzalez-de la Rosa, N E Beltran, E Pena-Mercado, M Garcia-Lorenzana, E Arechaga, C H Gonzalez-de la Rosa, N E Beltran, E Arechaga, E Pena-Mercado, M Garcia Lorenzana, N E Beltran
Gastric ischemia - reperfusion (I/R) injury is an important clinical problem, which is developed in more than 80% of critically ill patients. I/R is caused by interruption of blood supply to an organ or tissue followed by blood reflow into the exposed area, leading to multiple organ failure and death. Gastric reactance has been proposed to measure tissue injury caused by ischemia. The present study evaluates a new method to quantify gastric tissue damage due to I/R, and assess its relation to gastric reactance changes...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226740/automated-morphometric-description-of-human-corneal-endothelium-from-in-vivo-specular-and-confocal-microscopy
#19
F Scarpa, A Ruggeri, F Scarpa, A Ruggeri, A Ruggeri, F Scarpa
Corneal images acquired by in-vivo specular and confocal microscopy provide clinical information on the cornea endothelium health state. Indeed, the normal hexagonal shape of endothelial cells is usually affected by age and pathologies. At present, the analysis is based on manual or semi-automatic methods and the segmentation of a large number of endothelial cells is required for a meaningful estimation of the clinical parameters. We propose an automated method that detects the centers of endothelial cells by convolving the original image with customized two-dimensional kernels, derives a structure made by connected vertices from the recognized centers using the Euclidean distance, and fine-tunes the derived structure through a genetic algorithm, which combines information about the typical regularity of endothelial cells shape with the pixels intensity of the actual image...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28226718/automated-tracing-of-microglia-using-multilevel-thresholding-and-minimum-spanning-trees
#20
Mahmoud Abdolhoseini, Frederick Walker, Sarah Johnson, Mahmoud Abdolhoseini, Frederick Walker, Sarah Johnson, Sarah Johnson, Mahmoud Abdolhoseini, Frederick Walker
Microglia are immune cells of the central nervous system. Good knowledge about their morphology leads us to a better understanding of their functionality. In this article we propose an automated method to trace microglia in microscopy images. Our approach is powered by two main algorithms: multilevel thresholding (MT) and minimum spanning tree (MST). MT quantizes intensities of image pixels to several levels, then we sample each level with different rates to produce seed points. Each seed point belongs to a level and has a specific value, therefore they can be prioritized...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
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