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https://www.readbyqxmd.com/read/29786968/p62-mediated-autophagy-affects-nutrition-dependent-insulin-receptor-substrate-1-dynamics-in-3t3-l1-preadipocytes
#1
Hirobumi Igawa, Akihiro Kikuchi, Hirofumi Misu, Kiyo-Aki Ishii, Shuichi Kaneko, Toshinari Takamura
AIMS/INTRODUCTION: Previous studies have shown that the organism's nutritional status changes the protein levels of insulin receptor substrate 1 (IRS-1) in a tissue-specific manner. Although the mechanisms underlying the regulation of IRS-1 in the nutrient-rich conditions associated with diabetes and insulin resistance have been well studied, those under nutrient-poor conditions remain unknown. The aim of this study was to investigate how IRS-1 protein levels change depending on the nutritional status of 3T3-L1 preadipocytes...
May 22, 2018: Journal of Diabetes Investigation
https://www.readbyqxmd.com/read/29786920/functional-carbon-nanofibers-with-semi-embedded-titanium-oxide-particles-via-electrospinning
#2
Guoqing Chang, Wajid Ullah, Yunfeng Hu, Liwei Lin, Xu Wang, Chang-Zhi Li
The formulation of optoelectronic components into 1D nanostructures allows the promotion of new materials with multifunctionalities. In this work, it is demonstrated that new synthesis of photocatalytic carbon nanofiber decorated with semi-embedded titanium oxide (TiO2 ), namely, TiO2 @carbon fiber, is conveniently accessed through the electrospinning of polyacrylonitrile polymer and TiO2 particle comixture, and then followed by photon-activated self-erosion to expose the embedded TiO2 and carbonization. The hybrid nanofibers are characterized by field emission scanning electron microscopy, transmission electron microscopy, and X-ray diffraction analysis...
May 22, 2018: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/29786860/effect-of-cold-plasma-on-maintaining-the-quality-of-chub-mackerel-scomber-japonicus-biochemical-and-sensory-attributes
#3
Jing Chen, Sheng-Zhe Wang, Jun-Yu Chen, Dong-Zhi Chen, Shang-Gui Deng, Bin Xu
BACKGROUND: Atmospheric cold plasma (ACP) has emerged as a potential alternative to traditional methods for non-thermal food decontamination. However, little data are available about ACP treatment for seafood. In this study, dielectric barrier discharge (DBD) was applied to generate CP, and the aim of the study was to investigate the effectiveness of DBD-ACP on improving the quality of chub mackerel on the basis of chemical, microbial, and sensory characteristics. RESULTS: The effect of DBD-ACP on the quality of chub mackerel (Scomber japonicus) during storage was examined...
May 21, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29786842/correlated-time-variation-of-bulk-microstructure-and-rheology-in-asphalt-binders
#4
A Ramm, N Sakib, A Bhasin, M C Downer
We use near-infrared dark-field optical microscopy to probe isothermal time variation of the volume fraction of naturally-occurring, subsurface microstructures in PG 64-22 asphalt binders at temperature T=30∘C, following a rapid heating (cooling) increment |ΔT|=20∘C from initial temperature T0=10∘C(50∘C). We compare these microstructure variations with isothermal time variations of the magnitude |G30∗(t)| of the bulk complex shear modulus measured for identical sample conditions with a Dynamic Shear Rheometer...
May 22, 2018: Journal of Microscopy
https://www.readbyqxmd.com/read/29786838/edible-gum-phenolic-lipid-incorporated-gluten-films-for-food-packaging
#5
Rui Liu, Xu Cong, Yingshi Song, Tao Wu, Min Zhang
The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings. The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined. FG and OPCs improved the WVP properties of the gluten films, whereas LA significantly improved the oxygen-barrier properties. The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29786837/high-intensity-ultrasound-for-salmonella-enteritidis-inactivation-in-culture-and-liquid-whole-eggs
#6
Chayapa Techathuvanan, Doris Helen D'Souza
High intensity ultrasound (HIU) continues to be researched as a nonthermal inactivation technology of appeal to food manufacturers. The advantages of HIU include maintenance of product quality, freshness, product homogenization, along with simultaneous inactivation of pathogens. Besides, it is simple, relatively inexpensive, and easily adaptable to most processing environments. This study investigated the effect of HIU for Salmonella Enteritidis inactivation in culture and liquid whole eggs (LWEs) to decrease egg-associated outbreaks...
May 22, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29786742/a-novel-alendronate-functionalized-nanoprobe-for-simple-colorimetric-detection-of-cancer-associated-hypercalcemia
#7
Abhishek Sahu, Youngmin Hwang, Cristian Vilos, Jong-Min Lim, Sunghyun Kim, Won Il Choi, Giyoong Tae
The calcium (Ca2+) ion concentration in the blood serum is tightly regulated, and any abnormalities in the level of serum calcium ions are associated with many potentially dangerous diseases. Thus, monitoring of the Ca2+ ion concentration in the blood serum is of fundamental importance. Gold nanoparticle (GNP)-based colorimetric biosensors have enormous potential in clinical diagnostic applications due to their simplicity, versatility, and unique optical properties. In this study, we have developed an alendronate functionalized gold nanoparticle (GNP-ALD) system for the measurement of Ca2+ ion concentration in biological samples...
May 22, 2018: Nanoscale
https://www.readbyqxmd.com/read/29786727/rational-design-of-multi-functional-gold-nanoparticles-with-controlled-biomolecule-adsorption-a-multi-method-approach-for-in-depth-characterization
#8
Isaac Ojea-Jiménez, Robin Capomaccio, Inês Osório, Dora Mehn, Giacomo Ceccone, Rohanah Hussain, Giuliano Siligardi, Pascal Colpo, François Rossi, Douglas Gilliland, Luigi Calzolai
Multi-functionalized nanoparticles are of great interest in biotechnology and biomedicine, especially for diagnostic and therapeutic purposes. However, at the moment the characterization of complex, multi-functional nanoparticles is still challenging and this hampers the development of advanced nanomaterials for biological applications. In this work, we have designed a model system consisting of gold nanoparticles functionalized with two differentially-terminated poly(ethylene oxide) ligands, providing both "stealth" properties and protein-binding capabilities to the nanoparticles...
May 22, 2018: Nanoscale
https://www.readbyqxmd.com/read/29786715/cancer-cell-membrane-coated-magnetic-nanoparticles-for-mr-nir-fluorescence-dual-modal-imaging-and-photodynamic-therapy
#9
Jiong Li, Xuandong Wang, Dongye Zheng, Xinyi Lin, Zuwu Wei, Da Zhang, Zhuanfang Li, Yun Zhang, Ming Wu, Xiaolong Liu
Theranostic nanoprobes integrated with dual-modal imaging and therapeutic functions, such as photodynamic therapy (PDT), have exhibited significant potency in cancer treatments due to their high imaging accuracy and non-invasive advantages for cancer elimination. However, biocompatibility and highly efficient accumulation of these nanoprobes in tumor are still unsatisfactory for clinical application. In this study, a photosensitizer -loaded magnetic nanobead with surface further coated with a layer of cancer cell membrane (SSAP-Ce6@CCM) was designed to improve the biocompatibility and cellular uptake and ultimately achieve enhanced MR/NIR fluorescence imaging and PDT efficacy...
May 22, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29786706/hg-ii-interactions-with-t-rich-regions-in-oligonucleotides-effects-of-positional-variations-on-the-electrochemical-properties
#10
Ajar Kamal, Renu Sharma, Zhe She, Heinz-Bernhard Kraatz
Hg(ii) binding to thymine-rich oligonucleotides (ODNs) is investigated electrochemically. The focus of this study is to probe the effects of position on the electrochemical response. For this purpose, three oligonucleotides were investigated in which the position of a hexa-thymine repeat is varied within a surface-supported oligonucleotide. The hexa repeats were placed in the top, middle, and bottom positions within the strand with respect to the gold surface. The effects were monitored by electrochemical impedance spectroscopy and scanning electrochemical microscopy...
May 22, 2018: Analyst
https://www.readbyqxmd.com/read/29786617/fabrication-of-hollow-boron-doped-diamond-nanostructure-via-electrochemical-corrosion-of-a-tungsten-oxide-template
#11
Young-Kyun Lim, Choong-Hyun Lee, Eung-Seok Lee, Dae-Soon Lim
In the study, a hollow boron-doped diamond (BDD) nanostructure electrode is fabricated to increase the reactive surface area for electrochemical applications. Tungsten oxide nanorods are deposited on the silicon substrate as a template by the hot filament chemical vapor deposition (HFCVD) method. The template is coated with a 100 nm BDD layer deposited by HFCVD to form a core-shell nanostructure. The WOx core is finally electrochemically dissolved to form hollow BDD nanostructure. The fabricated hollow BDD nanostructure electrode is investigated via scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Raman spectroscopy...
May 22, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29786447/thickness-tunable-synthesis-of-ultrathin-type-ii-dirac-semimetal-ptte-2-single-crystals-and-their-thickness-dependent-electronic-properties
#12
Huifang Ma, Peng Chen, Bo Li, Jia Li, Ruoqi Ai, Zhengwei Zhang, Guangzhuang Sun, Kangkang Yao, Zhaoyang Lin, Bei Zhao, Ruixia Wu, Xuwan Tang, Xidong Duan, Xiangfeng Duan
The recent discovery of topological semimetals has stimulated extensive research interest due to their unique electronic properties and novel transport properties related to a chiral anomaly. However, the studies to date are largely limited to bulk crystals and exfoliated flakes. Here, we report the controllable synthesis of ultrathin two-dimensional (2D) platinum telluride (PtTe2 ) nanosheets with tunable thickness and investigate the thickness-dependent electronic properties. We show that PtTe2 nanosheets can be readily grown, using a chemical vapor deposition approach, with a hexagonal or triangular geometry and a lateral dimension of up to 80 μm, and the thickness of the nanosheets can be systematically tailored from over 20 to 1...
May 22, 2018: Nano Letters
https://www.readbyqxmd.com/read/29786426/influence-of-enzyme-quantity-and-distribution-on-the-self-propulsion-of-non-janus-urease-powered-micromotors
#13
Tania Patiño, Natalia Feiner-Gracia, Xavier Arque, Albert Miguel-López, Anita Jannasch, Tom Stumpp, Erik Schäffer, Lorenzo Albertazzi, Samuel Sanchez
The use of enzyme catalysis to power micro and nanomachines offers unique features such as biocompatibility, versatili-ty and fuel bioavailability. Yet, the key parameters underlying the motion behavior of enzyme-powered motors are not completely understood. Here, we investigate the role of enzyme distribution and quantity on the generation of active mo-tion. Two different micromotor architectures based on either polystyrene (PS) or polystyrene coated with a rough silicon dioxide shell (PS@SiO2) were explored...
May 22, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29786109/engulfment-of-platelets-delays-endothelial-cell-aging-via-girdin-and-its-phosphorylation
#14
Yong Lan, Yongjun Li, Dajun Li, Peng Li, Jiyang Wang, Yongpeng Diao, Guodong Ye, Yangfang Li
Endothelial cells are critical in angiogenesis and maintain the homeostasis of the blood‑brain barrier (BBB). Platelets (PLTs) are essential in vascular biology, including angiogenesis. The present study aimed to investigate the effect of PLTs on the aging of endothelial cells. Human brain microvascular endothelial cells (HBMECs) and human astrocytes were co‑cultured to mimic the BBB. Transmission electron microscopy was used to observe the engulfment of PLTs. Confocal microscopy was used to observe the co‑localization of PLTs, girders of actin filament (girdin) and phosphorylated (p‑)girdin...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786093/author-correction-high-speed-volumetric-imaging-of-neuronal-activity-in-freely-moving-rodents
#15
Oliver Skocek, Tobias Nöbauer, Lukas Weilguny, Francisca Martínez Traub, Chuying Naomi Xia, Maxim I Molodtsov, Abhinav Grama, Masahito Yamagata, Daniel Aharoni, David D Cox, Peyman Golshani, Alipasha Vaziri
In the version of this Brief Communication originally published online, ref. 21 included details for a conference paper (Pegard, N. C. et al. Paper presented at Novel Techniques in Microscopy: Optics in the Life Sciences, Vancouver, BC, Canada, 12-15 April 2015). The correct reference is the following: Pégard, N. C. et al. Optica 3, 517-524 (2016). This error has been corrected in the print, HTML and PDF versions of the paper.
May 21, 2018: Nature Methods
https://www.readbyqxmd.com/read/29785840/exploring-photoinactivation-of-microbial-biofilms-using-laser-scanning-microscopy-and-confined-two-photon-excitation
#16
Hanna Thomsen, Fabrice E Graf, Anne Farewell, Marica B Ericson
One pertinent complication in bacterial infection is the growth of biofilms, i.e., communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are required. Herein, we present an approach employing multiphoton microscopy complemented with Airyscan super-resolution microscopy, to visualize the distribution of curcumin in Staphylococcus epidermidis biofilms...
May 21, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29785769/distinct-hypericum-perforatum-l-total-extracts-exert-different-antitumour-activity-on-erythroleukemic-k562-cells
#17
Elena Valletta, Annamaria Rinaldi, Mario Marini, Ornella Franzese, Gianna Roscetti
Total flower extracts of Hypericum perforatum L. obtained with 3 different solvent systems were tested on tumour cell line cultures by comparing two groups of plants harvested in different times and places. The extracts, characterized according to the spectroscopic profile and the hypericin content, were tested on the growth and apoptotic death of K562 cells, a human erythroleukemic cell line. Growth and apoptosis were analysed by viable cell count, flow cytometry, and fluorescence microscopy at 6, 24, and 48 hr of culture following 1 hr exposure to the extracts under investigation...
May 22, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29785759/effect-of-2-94-%C3%A2%C2%B5m-er-yag-laser-on-the-chemical-composition-of-hard-tissues
#18
Abtesam Aljdaimi, Hugh Devlin, Mark Dickinson, Abdullatif Alfutimie
The aim was to investigate the effect of the Er-YAG laser radiation on morphology and chemical composition of enamel, dentin, and bone. The specimens of the three groups were irradiated with a very long pulse mode (VLP) of 2.94 µm Er-YAG laser with 100 mJ pulse energy and energy density of 8.42 J/ cm2 for 30 s, at a repetition rate of 15 Hz. The organic and inorganic content of the samples were investigated by Fourier Transforms Infrared spectroscopy (FTIR). The morphological characteristics were investigated with scanning electron microscopy (SEM) and elemental analysis (calcium and phosphorus) with energy-dispersive X-ray spectroscopy (EDX)...
May 21, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29785742/synthesis-and-characterization-of-a-new-bifunctionalized-fluorescent-and-amphiphilic-molecule-to-recruit-sh-containing-molecules-to-membranes
#19
Monique Mertens, Malte Hilsch, Ivan Haralampiev, Rudolf Volkmer, Pablo Wessig, Peter Mueller
The present study describes the synthesis and characterization of an amphiphilic construct which is aimed to recruit SH-containing molecules to membranes. The construct consists of (i) an aliphatic chain to enable the anchoring within membranes, (ii) a maleimide moiety to react with the sulfhydryl group of a soluble (bio)molecule, and (iii) a fluorescence moiety to follow the construct by fluorescence spectroscopy and microscopy. It is shown, that the construct can be incorporated into preformed membranes allowing the application of the approach with biological membranes...
May 22, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29785608/reuse-of-organobentonites-with-a-carbon-dioxide-responsive-solvent
#20
Ling-Yu Luan, Li Zhang, Li-Hong Wang
Synthesized organobentonite (SOB), montmorillonite (MMT), and commercial organobentonite (COB) were used as adsorbents for paraffin oil, a model pollutant in land-based oil spills and oil pipeline rupture. The characterization of clays was carried out with scanning electron microscopy (SEM), thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). After adsorption, oil was separated from adsorbents with a carbon dioxide responsive solvent N,N-dimethylcyclohexylamine (DMCHA), and DMCHA was subsequently separated from paraffin oil upon CO2 bubbling instead of distillation with high energy cost...
May 22, 2018: Environmental Science and Pollution Research International
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