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https://www.readbyqxmd.com/read/28432999/effect-of-intra-oral-aging-on-t%C3%A2-m-phase-transformation-microstructure-and-mechanical-properties-of-y-tzp-dental-ceramics
#1
Luciana Meireles Miragaya, Renato Bastos Guimarães, Rodrigo Othávio de Assunção E Souza, Glauco Dos Santos Botelho, José Guilherme Antunes Guimarães, Eduardo Moreira da Silva
The aim of the present study was to evaluate the influence of intra-oral aging on the tetragonal-to-monoclinic (t→m) phase transformation of two Y-TZP dental ceramics - Lava Frame (Frame) and Lava Plus (Plus) - and determine the impact of this response on their microstructures and mechanical properties: flexural strength, Young's modulus, microhardness and fracture toughness. Standardized ceramic specimens were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM)...
April 13, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28432996/toxicological-effects-of-phenol-on-four-marine-microalgae
#2
Weiyan Duan, Fanping Meng, Yufei Lin, Guoshan Wang
The toxic effects of phenol on four marine microalgae (Dunaliella salina, Platymonas subcordiformis, Phaeodactylum tricornutum Bohlin, and Skeletonema costatum) were evaluated. The 96h EC50 values were 72.29, 92.97, 27.32, and 27.32mgL(-1), respectively, which were lower than those values of freshwater microalgae reported in the literature. During a 96-h exposure to a sub-lethal concentration of phenol (1/2 96h EC50) with green alga (D. salina) and diatom (S. costatum), reactive oxygen species (ROS) accumulation, and chlorophyll a (Chl a) content decrease were simultaneously observed in diatom cells after 48h treatment...
April 13, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/28432978/cadmium-hampers-salt-tolerance-of-sesuvium-portulacastrum
#3
Mariem Wali, Soledad Martos, Laura Pérez-Martín, Chedly Abdelly, Tahar Ghnaya, Charlotte Poschenrieder, Benet Gunsé
It is well known that salinity reduces cadmium toxicity in halophytes. However, the possible interference of Cd with the mechanisms of salt tolerance is poorly explored. The aim of this study was to see whether Cd affects salt tolerance mechanisms in the halophyte Sesuvium portulacastrum. S. portulacastrum plants obtained from cuttings were grown in hydroponics for 3 weeks and then exposed to low (0.09 mM) or moderate (200 mM) NaCl concentrations, alone or in combination with 25 μM CdCl2. Microscopy observation revealed two strategies of salt tolerance: euhalophytism and secretion of salt by bladder cells...
April 13, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28432958/membrane-fusion-between-baculovirus-budded-virus-enveloped-particles-and-giant-liposomes-generated-using-a-droplet-transfer-method-for-the-incorporation-of-recombinant-membrane-proteins
#4
Misako Nishigami, Takaaki Mori, Masahiro Tomita, Kingo Takiguchi, Kanta Tsumoto
Giant proteoliposomes are generally useful as artificial cell membranes in biochemical and biophysical studies, and various procedures for their preparation have been reported. We present here a novel preparation technique that involves the combination of i) cell-sized lipid vesicles (giant unilamellar vesicles, GUVs) that are generated using the droplet-transfer method, where lipid monolayer-coated water-in-oil microemulsion droplets interact with oil/water interfaces to form enclosed bilayer vesicles, and ii) budded viruses (BVs) of baculovirus (Autographa californica nucleopolyhedrovirus) that express recombinant transmembrane proteins on their envelopes...
April 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28432955/the-calorimetric-properties-of-liposomes-determine-the-morphology-of-dried-droplets
#5
Jorge González-Gutiérrez, Rosendo Pérez-Isidoro, M I Pérez-Camacho, J C Ruiz-Suárez
The evaporation of liquid droplets deposited on a substrate is a very complex phenomenon. Driven by capillary and Marangoni flows, particle-particle and particle-substrate interactions, the deposits they leave are vestiges of such complexity. We study the formation of patterns during the evaporation of liposome suspension droplets deposited on a hydrophobic substrate at different temperatures. We observed that as we change the temperature of the substrate, a morphological phase transition occurs at a given temperature Tm...
April 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28432918/a-quinoline-based-cu-2-ion-complex-fluorescence-probe-for-selective-detection-of-inorganic-phosphate-anion-in-aqueous-solution-and-its-application-to-living-cells
#6
Yanpeng Dai, Peng Wang, Jiaxin Fu, Kun Yao, Kuoxi Xu, Xiaobin Pang
A quinaldine functionalized probe QP has been designed and synthesized. It exhibited selective turn-off fluorescence response toward Cu(2+) ion over most of the biologically important ions at physiological pH. The binding ratio of the probe QP and Cu(2+) ion was determined to be 1:1 through fluorescence titration, Job's plot and ESI-MS. The binding constant (K) of Cu(2+) to probe QP was found to be 2.12×10(4)M(-1). Further, the Cu(2+) ensemble of probe QP was found to respond H2PO4(-) and HPO4(2-) among other important biological anions via fluorescence turn-on response at physiological pH...
April 18, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28432913/spontaneously-formed-redox-and-ph-sensitive-polymersomes-by-mpeg-based-cytocompatible-random-copolymers
#7
Partha Laskar, Joykrishna Dey, Sudip Kumar Ghosh
Stimuli-sensitive polymersomes are one of the important vehicles and have been extensively studied as smart drug delivery system. Polymersomes have added advantage over the micelles because of having the ability to carry not only hydrophobic but also hydrophilic guest in their aqueous core. Among various stimuli, the change of pH and redox reaction is very important for drug delivery purpose especially for anticancer drug. Therefore, in this work, two poly(ethylene glycol) methyl ether methacrylate (mPEG) containing hydrophilic random anionic copolymers, poly[(2-hydroxyethyl methacrylate-3,3'-dithiodipropanoic acid)x-co-(poly(ethylene glycol) methyl ether methacrylate)y], poly[(HEMA-DTDPA)x-co-mPEGy] with different copolymer ratios were designed and synthesized...
April 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28432905/statistical-analysis-of-dislocations-and-dislocation-boundaries-from-ebsd-data
#8
C Moussa, M Bernacki, R Besnard, N Bozzolo
Electron BackScatter Diffraction (EBSD) is often used for semi-quantitative analysis of dislocations in metals. In general, disorientation is used to assess Geometrically Necessary Dislocations (GNDs) densities. In the present paper, we demonstrate that the use of disorientation can lead to inaccurate results. For example, using the disorientation leads to different GND density in recrystallized grains which cannot be physically justified. The use of disorientation gradients allows accounting for measurement noise and leads to more accurate results...
April 14, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28432904/optimising-multi-frame-adf-stem-for-high-precision-atomic-resolution-strain-mapping
#9
Lewys Jones, Sigurd Wenner, Magnus Nord, Per Harald Ninive, Ole Martin Løvvik, Randi Holmestad, Peter D Nellist
Annular dark-field scanning transmission electron microscopy is a powerful tool to study crystal defects at the atomic scale but historically single slow-scanned frames have been plagued by low-frequency scanning-distortions prohibiting accurate strain mapping at atomic resolution. Recently, multi-frame acquisition approaches combined with post-processing have demonstrated significant improvements in strain precision, but the optimum number of frames to record has not been explored. Here we use a non-rigid image registration procedure before applying established strain mapping methods...
April 15, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28432657/in-vitro-assay-for-measuring-real-time-topotecan-release-from-liposomes-release-kinetics-and-cellular-internalization
#10
Roger Gilabert-Oriol, Lina Chernov, Malathi Anantha, Wieslawa H Dragowska, Marcel B Bally
Topotecan is a drug that is under investigation for the treatment of neuroblastoma and has been encapsulated into liposomes to improve its therapeutic efficacy. However, liposomal formulations still need to be optimized for drug retention and new techniques to measure drug release are required to better understand this process. Here, a novel in vitro method based on fluorescence de-quenching and an automated microscopy imaging platform were developed for monitoring, in real time, the release of topotecan from a liposomal formulation...
April 21, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28432501/high-scale-yield-of-nano-hydroxyapatite-through-combination-of-mechanical-activation-and-chemical-dispersion
#11
Xueling Gao, Chunchu Dai, Weiwei Liu, Yumei Liu, Ru Shen, Xiaotong Zheng, Ke Duan, Jie Weng, Shuxin Qu
The aim of this study is to develop a simple, convenient and effective approach to synthesize nano-sized hydroxyapatite (nano-HA) at high-scale yield. Nano-HA was wet synthesized in the presence or absence of alendronate sodium (ALN), one of bisphosphonates for anti-osteoporotic. Then aged and washed nano-HA precipitate was directly treated by mechanical activation combined with the chemical dispersion of ALN to prevent the agglomeration of nano-HA. ALN acted not only as a chemical dispersant but also as an orthopedic drug...
June 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28432452/fluorescent-angioscopic-imaging-of-calcium-phosphate-tribasic-precursor-of-hydroxyapatite-the-major-calcium-deposit-in-human-coronary-plaques
#12
Takanobu Kobayashi, Osamu Nakagawa, Seiichiro Shirai, Ei Shimoyama, Nobuyuki Hiruta, Yasumi Uchida
Coronary calcification is a risk factor for ischemic heart disease. Hydroxyapatite that is formed by polymerization from calcium phosphate tribasic (CPT) is the major constituent of coronary calcium deposits. If CPT could be visualized, coronary calcification could be predicted and prevented. We discovered that when CPT and collagen I, the main constituent of collagen fibers, are mixed with lac dye (LD) and then exposed to fluorescent light excited at 345 ± 15 nm and emitted at 420 nm, a purple fluorescence that is characteristic of CPT only is elicited...
April 21, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28432366/exploring-quantum-griffiths-phase-in-ni1-x-v-x-nanoalloys
#13
Priyadarsini Swain, Suneel K Srivastava, Sanjeev K Srivastava
Metallic Ni1-x V x alloys exhibit a ferromagnetic to paramagnetic disordered quantum phase transition in bulk. Such a phase transition is accompanied by a quantum Griffiths phase (QGP), featuring fractional power-law temperature dependences of physical variables, like magnetic susceptibility and specific heat, at low temperatures. As nanoparticles (NP's) usually exhibit properties significantly different from their bulk counterparts, it is intriguing to explore the occurrence of quantum Griffiths phase in Ni1-x V x nanoalloys...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432219/optimized-cubic-protocol-for-3d-imaging-of-chicken-embryos-at-single-cell-resolution
#14
María Victoria Gómez-Gaviro, Evan Balaban, Diana Bocancea, María Teresa Lorrio, María Pompeiano, Manuel Desco, Jorge Ripoll, Juan José Vaquero
The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell-level resolution in whole early-stage chicken embryos, and in the whole brains of late-stage embryos.
April 21, 2017: Development
https://www.readbyqxmd.com/read/28432215/actomyosin-based-tissue-folding-requires-a-multicellular-myosin-gradient
#15
Natalie C Heer, Pearson W Miller, Soline Chanet, Norbert Stoop, Jörn Dunkel, Adam C Martin
Tissue folding promotes three-dimensional (3D) form during development. In many cases, folding is associated with myosin accumulation at the apical surface of epithelial cells, as seen in the vertebrate neural tube and the Drosophila ventral furrow. This type of folding is characterized by constriction of apical cell surfaces, and the resulting cell shape change is thought to cause tissue folding. Here, we use quantitative microscopy to measure the pattern of transcription, signaling, myosin activation, and cell shape in the Drosophila mesoderm...
April 21, 2017: Development
https://www.readbyqxmd.com/read/28431881/design-and-synthesis-of-1-2-3-triazolo-phenanthrene-hybrids-as-cytotoxic-agents
#16
Niggula Praveen Kumar, Shalini Nekkanti, S Sujana Kumari, Pankaj Sharma, Nagula Shankaraiah
A series of new 1,2,3-triazolo-phenanthrene hybrids has been synthesized by employing Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. These compounds were evaluated for their in vitro cytotoxic potential against various human cancer cell lines viz. lung (A549), prostate (PC-3 and DU145), gastric (HGC-27), cervical (HeLa), triple negative breast (MDA-MB-231, MDA-MB-453) and breast (BT-549, 4T1) cells. Among the tested compounds, 7d displayed highest cytotoxicity against DU145 cells with IC50 value of 1...
April 7, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28431810/in-vivo-ameliorative-effect-of-cerium-oxide-nanoparticles-in-isoproterenol-induced-cardiac-toxicity
#17
Shereen S El Shaer, Taher A Salaheldin, Nashwa M Saied, Sally M Abdelazim
BACKGROUND: Cerium oxide nanoparticles have gained much more attention especially in the field of nanomedicine. This work represents cerium oxide nanoparticles as a new prophylactic model for heart failure progression. OBJECTIVE: To investigate the potential protective effect of cerium oxide nanoparticles on Isoproterenol (ISO)-induced cardiac toxicity in rats. METHODS: Cerium oxide nanoparticles (5±1nm) were synthesized by reverse micelle method and characterized using High Resolution Transmission Electron Microscopy, X-Ray Diffraction and particle size analyzer...
April 18, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28431562/effects-of-the-common-polymorphism-in-the-human-aldehyde-dehydrogenase-2-aldh2-gene-on-the-lung
#18
Aoi Kuroda, Ahmed E Hegab, Gao Jingtao, Shuji Yamashita, Nobuyuki Hizawa, Tohru Sakamoto, Hideyasu Yamada, Satoshi Suzuki, Makoto Ishii, Ho Namkoong, Takanori Asakura, Mari Ozaki, Hiroyuki Yasuda, Junko Hamamoto, Shizuko Kagawa, Kenzo Soejima, Tomoko Betsuyaku
BACKGROUND: Aldehyde dehydrogenases (ALDHs) play a major role in detoxification of aldehydes. High expression of ALDHs is a marker for stem cells of many organs including the lungs. A common polymorphism in ALDH2 gene (ALDH2*2) results in inactivation of the enzyme and is associated with alcohol flushing syndrome and increased risk for cardiovascular and Alzheimer's diseases and some cancers. The effect of this ALDH2 polymorphism on the lung and its stem cells has not been thoroughly examined...
April 21, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28431540/prevention-of-advanced-glycation-end-products-formation-in-diabetic-rats-through-beta-cell-modulation-by-aegle-marmelos
#19
Rahman M Hafizur, Shahrukh Momin, Noor Fatima
BACKGROUND: Although the anti-diabetic activity of Aegle marmelos (AM) is known, however, its anti-glycation activity is not reported yet. In this study, we have investigated its anti-glycation activity under in vitro and in vivo conditions and determined possible mechanism(s) in streptozotocin-induced diabetic rats. METHODS: Effective dose of AM (400 mg/kg) was administrated orally to diabetic rats for 42 days. Thereafter, blood glucose, serum insulin, HbA1c, antioxidant status, and advanced glycation end-products (AGEs) were measured...
April 21, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28431523/the-utility-of-electron-microscopy-in-detecting-asbestos-fibers-and-particles-in-balf-in-diffuse-lung-diseases
#20
Takashi Kido, Yasuo Morimoto, Kazuhiro Yatera, Hiroshi Ishimoto, Takaaki Ogoshi, Keishi Oda, Kei Yamasaki, Toshinori Kawanami, Shohei Shimajiri, Hiroshi Mukae
BACKGROUND: In patients with diffuse lung diseases, differentiating occupational lung diseases from other diseases is clinically important. However, the value of assessing asbestos and particles in bronchoalveolar lavage fluid (BALF) in diffuse lung diseases by electron microscopy (EM) remains unclear. We evaluated the utility of EM in detecting asbestos fibers and particles in patients with diffuse lung diseases. METHODS: The BALF specimens of 107 patients with diffuse lung diseases were evaluated...
April 21, 2017: BMC Pulmonary Medicine
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