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https://www.readbyqxmd.com/read/29332353/aesculetin-6-7-dihydroxycoumarin-exhibits-potent-and-selective-antitumor-activity-in-human-acute-myeloid-leukemia-cells-thp-1-via-induction-of-mitochondrial-mediated-apoptosis-and-cancer-cell-migration-inhibition
#1
Jian Gong, Wei-Guo Zhang, Xiao-Fen Feng, Mei-Juan Shao, Chao Xing
PURPOSE: The main target of the present research was to examine the antitumor properties of aesculetin in human acute myeloid leukemia cancer cells (THP-1) and peripheral blood mono-nucleated cells (PBMCs) (used as normal cell line model) along with the determination of its effects on induction of apoptosis, inhibition of cancer cell migration and changes in Bcl-2/Bax protein expressions. METHODS: MTT colorimetric bioassay was performed to study the impact of this natural compound on cytotoxicity of both cell types...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29332344/in-vitro-antitumor-activity-of-guttiferone-a-in-human-breast-cancer-cells-is-mediated-via-apoptosis-mitochondrial-mediated-oxidative-stress-and-reactive-oxygen-species-production
#2
Hai-Ming Wu, Yu-Mei Li
PURPOSE: Breast cancer is the second most frequently diagnosed cancer and is considered as the main cause of cancer related death in females. It is estimated that about one-third of women with breast cancer develop metastases and eventually die of this disease. The main treatment options for breast cancer include surgical interventions followed by chemotherapy, hormonotherapy or radiation. However, the side effects associated with the treatment of breast cancer negatively affects the quality of patient's life...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29332340/imperatorin-shows-selective-antitumor-effects-in-sgc-7901-human-gastric-adenocarcinoma-cells-by-inducing-apoptosis-cell-cycle-arrest-and-targeting-pi3k-akt-m-tor-signalling-pathway
#3
J U Am, W J Gong, Y Su, Z B Mou
PURPOSE: The main purpose of the present study was to determine the anticancer properties of imperatorin - a naturally occurring coumarin compound - against SGC-7901 human gastric adenocarcinoma cells and the mouse fibroblast cell line 3T3 (normal cell line). METHODS: Imperatorin effects on apoptosis induction, cell cycle phase distribution and PI3K/Akt/m-TOR signalling pathways were studied. MTT cell viability assay examined the compound's cytotoxic potential, while inverted phase contrast microscopy and fluorescence microscopy techniques were used to study morphological changes induced in SGC-7901 cells by imperatorin...
November 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29332296/plant-cell-cultures-as-model-systems-to-study-programmed-cell-death
#4
Sara Cimini, Maria Beatrice Ronci, Elisabetta Barizza, Maria Concetta de Pinto, Vittoria Locato, Fiorella Lo Schiavo, Laura De Gara
The study of programmed cell death (PCD) activated in a certain group of cells is complex when analyzed in the whole plant. Plant cell suspension cultures are useful when investigating PCD triggered by environmental and developmental stimuli. Due to their homogeneity and the possibility to synchronize their responses induced by external stimuli, these cultures are used for studying the signaling pathways leading to PCD. The first problem in the analysis of PCD in cell cultures is the quantification of cell viability/death over time...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332289/detection-of-reactive-oxygen-and-nitrogen-species-ros-rns-during-hypersensitive-cell-death
#5
Laura C Terrón-Camero, Eliana Molina-Moya, María Sanz-Fernández, Luisa M Sandalio, María C Romero-Puertas
Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of H2O2 and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29332257/kynurenic-acid-and-its-derivatives-are-able-to-modulate-the-adhesion-and-locomotion-of-brain-endothelial-cells
#6
Eszter Lajkó, Bernadett Tuka, Ferenc Fülöp, István Krizbai, József Toldi, Kálmán Magyar, László Vécsei, László Kőhidai
The neuroprotective actions of kynurenic acid (KYNA) and its derivatives in several neurodegenerative disorders [characterized by damage to the cerebral endothelium and to the blood-brain barrier (BBB)] are well established. Cell-extracellular matrix (ECM) adhesion is supposedly involved in recovery of impaired cerebral endothelium integrity (endothelial repair). The present work aimed to investigate the effects of KYNA and its synthetic derivatives on cellular behaviour (e.g. adhesion and locomotion) and on morphology of the GP8 rat brain endothelial cell line, modeling the BBB endothelium...
January 13, 2018: Journal of Neural Transmission
https://www.readbyqxmd.com/read/29332246/an-historical-perspective-of-the-discovery-of-titin-filaments-part-2
#7
REVIEW
Cristobal G Dos Remedios
In 2017, a Special Issue of Biophysical Reviews was devoted to "Titin and Its Binding Partners. The issue contained a review: "An historical perspective of the discovery of titin filaments" by dos Remedios and Gilmour that was intended to be a history of the discovery of the giant protein titin, previously named connectin. The review took readers back to the earliest discovery of the so-called third filament component of skeletal and cardiac muscle sarcomeres and ended in 1969. Recently, my colleague Shin'ichi Ishiwata gently reminded me of two papers published in 1990 and 1993 that were unwittingly omitted from the original historical perspective...
January 13, 2018: Biophysical Reviews
https://www.readbyqxmd.com/read/29332224/phosphatidylserine-exposing-blood-and-endothelial-cells-contribute-to-the-hypercoagulable-state-in-essential-thrombocythemia-patients
#8
Dongxia Tong, Muxin Yu, Li Guo, Tao Li, Jihe Li, Valerie A Novakovic, Zengxiang Dong, Ye Tian, Junjie Kou, Yayan Bi, Jinghua Wang, Jin Zhou, Jialan Shi
The mechanisms of thrombogenicity in essential thrombocythemia (ET) are complex and not well defined. Our objective was to explore whether phosphatidylserine (PS) exposure on blood cells and endothelial cells (ECs) can account for the increased thrombosis and distinct thrombotic risks among mutational subtypes in ET. Using flow cytometry and confocal microscopy, we found that the levels of PS-exposing erythrocytes, platelets, leukocytes, and serum-cultured ECs were significantly higher in each ET group [JAK2, CALR, and triple-negative (TN) (all P < 0...
January 13, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29332212/measurement-of-cl-i-unaffected-by-the-cell-volume-change-using-mqae-based-two-photon-microscopy-in-airway-ciliary-cells-of-mice
#9
Yukiko Ikeuchi, Haruka Kogiso, Shigekuni Hosogi, Saori Tanaka, Chikao Shimamoto, Toshio Inui, Takashi Nakahari, Yoshinori Marunaka
MQAE is a 'non-ratiometric' chloride ion (Cl-)-quenched fluorescent indicator that is used to determine intracellular Cl- concentration ([Cl-]i). MQAE-based two-photon microscopy is reported to be a useful method to measure [Cl-]i, but it is still controversial because a change in cell volume may alter the MQAE concentration, leading to a change in the fluorescence intensity without any change in [Cl-]i. In an attempt to elucidate the effect or lack of effect of cell volume on MQAE concentration, we studied the effects of changes in cell volume, achieved by applying different levels of osmotic stress, on the intensity of MQAE fluorescence in airway ciliary cells...
January 13, 2018: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/29332155/prevalence-of-the-pfdhfr-and-pfdhps-mutations-among-asymptomatic-pregnant-women-in-southeast-nigeria
#10
Ekpereonne Esu, Costanza Tacoli, Prabhanjan Gai, Nicole Berens-Riha, Michael Pritsch, Thomas Loescher, Martin Meremikwu
Sulfadoxine-pyrimethamine (SP) is the recommended drug for intermittent preventive treatment of malaria in pregnancy in most of sub-Saharan Africa. Resistance to SP is related to mutations in the dhfr and dhps gene of Plasmodium falciparum. This study determined the prevalence of Pfdhfr and Pfdhps polymorphisms found in asymptomatic pregnant women attending antenatal care in Calabar, Nigeria. From October 2013 to November 2014, asymptomatic pregnant women attending antenatal care clinics were enrolled after obtaining informed consent...
January 13, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29332122/quantitative-study-of-the-macular-microvasculature-in-human-donor-eyes
#11
Paula K Yu, Zaid Mammo, Chandrakumar Balaratnasingam, Dao-Yi Yu
Purpose: To precisely quantify the macular microvasculature density using microperfusion and labeling techniques in human donor eyes. Such information may be useful in understanding the role of the macular microvasculature in coping with the metabolic requirements of the neurons in this densely packed region, and provide a reference point for clinical studies using recently developed optical imaging techniques. Methods: The macular microvasculature was perfusion-labeled in 18 human donor eyes and optical stacks collected from regions superior, temporal, inferior, and nasal to the foveola using confocal microscopy...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29332049/free-heme-and-amyloid-%C3%AE-a-fatal-liaison-in-alzheimer-s-disease
#12
Elisabeth Chiziane, Henriette Telemann, Martin Krueger, Juliane Adler, Jürgen Arnhold, A Alia, Jörg Flemmig
While the etiology of Alzheimer's disease (AD) is still unknown, an increased formation of amyloid-β (Aβ) peptide and oxidative processes are major pathological mechanism of the disease. The interaction of Aβ with free heme leads to the formation of peroxidase-active Aβ-heme complexes. However, enzyme-kinetic data and systematic mutational studies are still missing. These aspects were addressed in this study to evaluate the role of Aβ-heme complexes in AD. The enzyme-kinetic measurements showed peroxidase-specific pH- and H2O2-dependencies...
2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29331934/characterization-and-release-profile-of-mn-al-bearing-deposits-in-drinking-water-distribution-systems
#13
Guiwei Li, Yuanxun Ding, Hongfu Xu, Junwei Jin, Baoyou Shi
Inorganic contaminants accumulation in drinking water distribution systems (DWDS) is a great threat to water quality and safety. This work assessed the main risk factors for different water pipes and discovered the release profile of accumulated materials in a full scale distribution system frequently suffered from water discoloration problem. Physicochemical characterization of pipe deposits were performed using X-ray fluorescence, scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy...
January 9, 2018: Chemosphere
https://www.readbyqxmd.com/read/29331913/speciation-evolution-of-zinc-and-copper-during-pyrolysis-and-hydrothermal-carbonization-treatments-of-sewage-sludges
#14
Rixiang Huang, Bei Zhang, Emily M Saad, Ellery D Ingall, Yuanzhi Tang
Thermal and hydrothermal treatments are promising techniques for sewage sludge management that can potentially facilitate safe waste disposal, energy recovery, and nutrient recovery/recycling. Content and speciation of heavy metals in the treatment products affect the potential environmental risks upon sludge disposal and/or application of the treatment products. Therefore, it is important to study the speciation transformation of heavy metals and the effects of treatment conditions. By combining synchrotron X-ray spectroscopy/microscopy analysis and sequential chemical extraction, this study systematically characterized the speciation of Zn and Cu in municipal sewage sludges and their chars derived from pyrolysis (a representative thermal treatment technique) and hydrothermal carbonization (HTC; a representative hydrothermal treatment technique)...
January 4, 2018: Water Research
https://www.readbyqxmd.com/read/29331909/linking-composition-of-extracellular-polymeric-substances-eps-to-the-physical-structure-and-hydraulic-resistance-of-membrane-biofilms
#15
Peter Desmond, James P Best, Eberhard Morgenroth, Nicolas Derlon
The effect of extracellular polymeric substances (EPS) on the meso-scale physical structure and hydraulic resistance of membrane biofilms during gravity driven membrane (GDM) filtration was investigated. Biofilms were developed on the surface of ultrafiltration membranes during dead-end filtration at ultra-low pressure (70 mbar). Biofilm EPS composition (total protein, polysaccharide and eDNA) was manipulated by growing biofilms under contrasting nutrient conditions. Nutrient conditions consisted of (i) a nutrient enriched condition with a nutrient ratio of 100:30:10 (C: N: P), (ii) a phosphorus limitation (C: N: P ratio: 100:30:0), and (iii) a nitrogen limitation (C: N: P ratio: 100:0:10)...
December 27, 2017: Water Research
https://www.readbyqxmd.com/read/29331907/applying-ultraviolet-persulfate-uv-ps-pre-oxidation-for-controlling-ultrafiltration-membrane-fouling-by-natural-organic-matter-nom-in-surface-water
#16
Jiayu Tian, Cuiwei Wu, Huarong Yu, Shanshan Gao, Guibai Li, Fuyi Cui, Fangshu Qu
Membrane fouling is a recognized obstacle for the application of ultrafiltration (UF) for drinking water treatment. In this study, ultraviolet/persulfate (UV/PS) oxidation was employed as a pretreatment to control membrane fouling caused by natural organic matter (NOM) in surface water. The effects of UV/PS pretreatment on amounts and characteristics of NOM were investigated in terms of dissolved organic carbon, fluorescent spectrum, molecular weight distribution and hydrophobicity. UF membrane fouling during filtration of raw and pre-oxidized water was compared with transmembrane pressure development, and the fouled membranes were further characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR)...
January 5, 2018: Water Research
https://www.readbyqxmd.com/read/29331898/coadsorption-and-subsequent-redox-conversion-behaviors-of-as-iii-and-cr-vi-on-al-containing-ferrihydrite
#17
Zecong Ding, Fenglian Fu, Dionysios D Dionysiou, Bing Tang
Naturally occurring ferrihydrite often contains various impurities, and Al is one of the most prominent impurities. However, little is known about how these impurities impact the physical and chemical properties of ferrihydrite with respect to metal(loid) adsorption. In this study, a series of Al-containing ferrihydrites were synthesized and exposed to a mixed solution containing As(III) and Cr(VI). The results showed that the two contaminants can be quickly adsorbed onto the surface of Al-containing ferrihydrite under acidic and neutral conditions...
January 11, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29331822/synthesis-of-uio-66-oh-zirconium-metal-organic-framework-and-its-application-for-selective-extraction-and-trace-determination-of-thorium-in-water-samples-by-spectrophotometry
#18
Zahra Safaei Moghaddam, Massoud Kaykhaii, Mostafa Khajeh, Ali Reza Oveisi
In this study, a zirconium-based metal-organic framework (Zr-MOF), named UiO-66-OH, was synthesized by the solvo-thermal method and characterized by Fourier transform-infrared spectroscopy (FTIR), powder X-ray diffraction (PXRD), and scanning electron microscopy (SEM). This Zr-MOF was then employed as a sorbent for selective extraction and preconcentration of thorium ions after their complexation with 2‑(2,4‑dihydroxyphenyl)‑3,5,7‑trihydroxychromen‑4‑one (morin) from environmental water samples prior to its spectrophotometrical determination...
January 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29331821/influences-of-cdse-ncs-on-the-photovoltaic-parameters-of-bhj-organic-solar-cells
#19
Fatih Ongul, Sureyya Aydin Yuksel, Cagdas Allahverdi, Sinem Bozar, Mehmet Kazici, Serap Gunes
In this study, the high quality CdSe nanocrystals (NCs) capped with stearic acid were synthesized in a solvent and then purified four times by using the precipitation and redissolution process. The average size of the synthesized CdSe NCs was determined ~3.0nm via transmission electron microscopy (TEM) measurement and their corresponding optical band edge energy was also calculated as ~2.1eV using ultraviolet-visible (UV-Vis) absorption spectroscopy. The bulk heterojunction (BHJ) hybrid solar cells based on a ternary system including P3HT, PCBM and CdSe NCs at different weight concentrations (0wt%, 0...
January 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29331777/comparing-the-antifouling-effects-of-activated-carbon-and-tio2-in-ultrafiltration-membrane-development
#20
Qianyu Liu, Shaobin Huang, Yongqing Zhang, Shuaifei Zhao
We use activated carbon (AC) and titanium oxide (TiO2) nanomaterials as the additives to prepare four polyvinylidene fluoride (PVDF) based ultrafiltration membranes by nonsolvent induced phase separation. The surface properties (pore size, porosity, hydrophilicity and roughness) of the membranes are characterized by scanning electron microscopy, water contact angle measurement, and atomic force microscopy. The chemical properties of the membranes are evaluated by Fourier transform infrared spectroscopy with attenuated total reflection and X-ray diffraction...
January 6, 2018: Journal of Colloid and Interface Science
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