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https://www.readbyqxmd.com/read/28651342/emissions-of-volatile-odorous-metabolites-by-clostridium-perfringens-in-vitro-study-using-two-broth-cultures
#1
Nishchal K Sharma, Chuck Keerqin, Shu-Biao Wu, Mingan Choct, Robert A Swick
An in vitro study was carried out to investigate the volatile odorous metabolites produced by necrotic enteritis inducing Clostridium perfringens (Cp) type A field strain (EHE-NE18) cultured in two broth media (thioglycollate broth, brain heart infusion broth) at three levels (0, 103 cfu/mL, 106 cfu/mL) using 2 × 3 factorial arrangement of treatments, with each replicated three times. The culture media with and without Cp was freshly prepared on the day of measurement and the culture headspace concentration of odorants was measured using selected ion-flow tube mass spectrometry (SIFT-MS)...
June 23, 2017: Poultry Science
https://www.readbyqxmd.com/read/28651210/associations-between-perfluorinated-alkyl-acids-in-blood-and-ovarian-follicular-fluid-and-ovarian-function-in-women-undergoing-assisted-reproductive-treatment
#2
Jessica A McCoy, Jacqueline T Bangma, Jessica L Reiner, John A Bowden, John Schnorr, Michael Slowey, Thomas O'Leary, Louis J Guillette, Benjamin B Parrott
Endocrine disrupting contaminants, in combination with other environmental variables, are associated with altered reproductive health. Assisted reproductive technology (ART) procedures offer valuable opportunities to explore the connections between environmental contaminants in the ovarian microenvironment and measures of fertility, including impaired responsiveness to gonadotropins. Here, we investigate an emerging class of environmental contaminants, the perfluorinated alkyl acids (PFAAs), to determine whether ovarian contaminant levels are associated with measures of ovarian responsiveness and fertility outcomes in a South Carolina population of women undergoing ART...
June 23, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28651176/forensic-applications-of-direct-analysis-in-real-time-dart-coupled-to-q-orbitrap-tandem-mass-spectrometry-for-the-in-situ-analysis-of-pigments-from-paint-evidence
#3
Tai-Hung Chen, Shu-Pao Wu
The accurate examination of paint fragments obtained from an accident, such as those obtained from vehicles involved in a hit-and-run case, is often critical in forensic investigations. However, organic pigments are typically minor components of automotive coatings, which makes discrimination difficult. In this study, direct analysis in real time coupled to Q-orbitrap tandem mass spectrometry (DART-MS) was employed to detect a wide range of common organic pigments in vehicle paints. Twelve common organic pigments used in vehicle paints, such as red, yellow, orange, and purple, were tested, and a database was constructed for future examinations of vehicle paint...
June 9, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28651172/simultaneous-determination-of-cis-permethrin-and-trans-permethrin-in-rat-plasma-and-brain-tissue-using-gas-chromatography-negative-chemical-ionization-mass-spectrometry
#4
Shirin Hooshfar, Darren R Gullick, Michael R Linzey, Tanzir Mortuza, Mona Hamdy Abdel Rahman, Clinton A Rogers, James V Bruckner, Catherine A White, Michael G Bartlett
A sensitive method for the simultaneous determination of cis-permethrin (cis-PERM) and trans-permethrin (trans-PERM) in small volumes (100μL) of rat plasma and brain homogenate was developed, using a liquid-liquid extraction for sample preparation and gas chromatography-negative chemical ionization mass spectrometry (GCNCI-MS) for detection. Quantitation of trace levels of the insecticide in small volumes of biological samples is essential to support toxicokinetic studies in small animals. There are currently no validated methods in the literature for determining cis-PERM and trans- PERM in volumes as low as 100μL of rat plasma or brain homogenate...
June 13, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28651169/anticancer-activity-of-hydroxy-and-sulfonamide-azobenzene-platinum-ii-complexes-in-cisplatin-resistant-ovarian-cancer-cells
#5
Katia G Samper, Sierra C Marker, Pau Bayón, Samantha N MacMillan, Ivan Keresztes, Òscar Palacios, Justin J Wilson
The syntheses of three platinum(II) complexes bearing sulfonamide- ( (E)-2-(4-methylphenylsulfonamido)-2',6'-difluoroazobenzene, HL1) and hydroxy-azo-2,6-difluorobenzene ((E)-2-((2,6-difluorophenyl)diazenyl)phenol, HL2) bidentate ligands is described. These complexes, [Pt(L1)(DMSO)Cl] (1), [Pt(L2)(DMSO)Cl] (2), and [Pt(L2)2] (3), were characterized by multinuclear NMR spectroscopy, mass spectrometry, and X-ray crystallography. Despite bearing azobenzene functional groups, none of the three complexes undergo photoisomerization...
June 17, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28651142/fast-pyrolysis-of-durian-durio-zibethinus-l-shell-in-a-drop-type-fixed-bed-reactor-pyrolysis-behavior-and-product-analyses
#6
Y L Tan, A Z Abdullah, B H Hameed
Durian shell (DS) was pyrolyzed in a drop-type fixed-bed reactor to study the physicochemical properties of the products. The experiment was carried out with different particle sizes (up to 5mm) and reaction temperatures (250-650°C). The highest bio-oil yield was obtained at 650°C (57.45wt%) with DS size of 1-2mm. The elemental composition and higher heating value of the feedstock, bio-oil (650°C), and bio-char (650°C) were determined and compared. The compositions of product gases were determined via gas chromatography with thermal conductivity detector...
June 23, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651109/separation-of-furostanol-saponins-by-supercritical-fluid-chromatography
#7
Jie Yang, Lingling Zhu, Yang Zhao, Yongwei Xu, Qinglong Sun, Shuchen Liu, Chao Liu, Baiping Ma
Supercritical fluid chromatography (SFC) has good separation efficiency and is suitable for separating weakly polar compounds. Furostanol saponins, as an important kind of steroidal saponins, generally have two sugar chains, which are polar and hydrophilic. The hydroxyl group at the C-22 position of furostanol saponins is active and easily reacts with lower alcohols under appropriate conditions. The separation of hydrophilic furostanol saponins was tested by SFC in this study. The effects of chromatographic conditions on the separation of the mixed furostanol saponins and their hydroxyl derivatives at the C-22 position were studied...
June 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28650900/a-high-throughput-hplc-ms-ms-assay-for-the-detection-quantification-and-simultaneous-structural-confirmation-of-136-drugs-and-metabolites-in-human-urine
#8
Jacek Klepacki, Baharak Davari, Marion Boulet, Ryan Lizarraga, Uwe Christians
BACKGROUND: Due to its superior sensitivity and specificity, multi-analyte high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) has become the gold standard in clinical toxicology. Although several qualitative and quantitative LC-MS/MS assays on various mass spectrometry platforms have been described in the literature, the majority of these methods either analyze only a limited number of compounds, and/or require tedious and time-consuming sample preparation...
June 22, 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28650662/glucose-restriction-combined-with-autophagy-inhibition-and-chemotherapy-in-hct-116-spheroids-decreases-cell-clonogenicity-and-viability-regulated-by-tumor-suppressor-genes
#9
Monica M Schroll, Gabriel J LaBonia, Katelyn R Ludwig, Amanda B Hummon
Drug resistance is a prevalent phenomenon that decreases the efficacy of cancer treatments and contributes to cancer progression and metastasis. Weakening drug resistant cancer cells prior to chemotherapy is a potential strategy to combat chemoresistance. One approach to damage resistant cancer cells is modulation of nutritional intake. The combination of nutrient restriction with targeted compound treatment results in pronounced molecular changes. This study provides valuable information about augmenting existing chemotherapeutic regimes with simultaneous glucose restriction and autophagy inhibition in colorectal cancer cells...
June 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28650653/growth-and-metabolic-responses-of-rice-oryza-sativa-l-cultivated-in-phosphorus-deficient-soil-amended-with-tio2-nanoparticles
#10
Zahra Zahra, Naima Waseem, Rubab Zahra, Hwanhui Lee, Mohsin Ali Badshah, Arshad Mehmood, Hyung-Kyoon Choi, Muhammad Arshad
Plants have natural ability to withstand stress conditions through metabolic adjustments. The present study aimed at investigating effects of Titanium dioxide nanoparticles (TiO2 NPs) application (0, 25, 50, 150, 250, 500 and 750 mg kg-1) in phosphorus deficient soil in terms of growth responses, P contents and metabolic alterations in rice. TiO2 NPs application increased shoot length up to 14.5%. Phosphorus contents in rice roots, shoots, and grains were increased by 2.6-, 2.4-, and 1.3-fold, respectively at 750 mg kg-1 of TiO2 NPs...
June 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28650563/unusual-lipid-a-from-a-cold-adapted-bacterium-detailed-structural-characterization
#11
Maria Michela Corsaro, Angela Casillo, Marcello Ziaco, Buko Lindner, Ermenegilda Parrilli, Dominik Schwudke, Aurora Holgado, Lynn Verstrepen, Filomena Sannino, Rudi Beyaert, Rosa Lanzetta, Maria Luisa Tutino
Colwellia psychrerythraea 34H is a Gram-negative cold-adapted microorganism that adopts many strategies to cope with the limitations due to the low temperatures of its habitat. In this study, we reported the complete characterization of the lipid A moiety from the lipopolysaccharide of Colwellia, to find out if structural features could be linked to the cold-adaptation strategy. The lipid A and its partially deacylated derivative were completely characterized by means of high resolution mass spectrometry, NMR spectroscopy, and chemical analysis...
June 26, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28650464/%C3%AE-catenin-downregulates-dicer-to-promote-ovarian-cancer-metastasis
#12
S K Y To, A S C Mak, Y M Eva Fung, C-M Che, S-S Li, W Deng, B Ru, J Zhang, A S T Wong
Ovarian cancer is a nearly uniform lethal disease and its highly aggressive metastatic phenotype portends a poor prognosis. Lack of a well-controlled, relevant experimental model has been a major obstacle to identifying key molecules causing metastasis. Here we describe the creation of a new isogenic model of spontaneous human ovarian cancer metastasis exhibiting opposite phenotypes-highly metastatic (HM) and non-metastatic (NM)-both in vitro and in vivo. HM was unique in its ability to metastasize consistently to the peritoneum, mimicking the major dissemination route of human ovarian cancer...
June 26, 2017: Oncogene
https://www.readbyqxmd.com/read/28650391/proteins-differentially-expressed-in-the-pancreas-of-hepatic-alcohol-dehydrogenase-deficient-deer-mice-fed-ethanol-for-3-months
#13
Kamlesh K Bhopale, Samir M Amer, Lata Kaphalia, Kizhake V Soman, John E Wiktorowicz, Ghulam A Shakeel Ansari, Bhupendra S Kaphalia
OBJECTIVES: The aim of this study was to identify differentially expressed proteins in the pancreatic tissue of hepatic alcohol dehydrogenase-deficient deer mice fed ethanol to understand metabolic basis and mechanism of alcoholic chronic pancreatitis. METHODS: Mice were fed liquid diet containing 3.5 g% ethanol daily for 3 months, and differentially expressed pancreatic proteins were identified by protein separation using 2-dimensional gel electrophoresis and identification by mass spectrometry...
June 22, 2017: Pancreas
https://www.readbyqxmd.com/read/28650319/somatostatin-binds-to-the-human-amyloid-%C3%AE-peptide-and-favors-the-formation-of-distinct-oligomers
#14
Hansen Wang, Lisa D Muiznieks, Punam Ghosh, Declan Williams, Michael Solarski, Andrew Fang, Alejandro Ruiz-Riquelme, Régis Pomès, Joel C Watts, Avi Chakrabartty, Holger Wille, Simon Sharpe, Gerold Schmitt-Ulms
The amyloid β peptide (Aβ) is a key player in the etiology of Alzheimer disease (AD), yet a systematic investigation of its molecular interactions has not been reported. Here we identified by quantitative mass spectrometry proteins in human brain extract that bind to oligomeric Aβ1-42 (oAβ1-42) and/or monomeric Aβ1-42 (mAβ1-42) baits. Remarkably, the cyclic neuroendocrine peptide somatostatin-14 (SST14) was observed to be the most selectively enriched oAβ1-42 binder. The binding interface comprises a central tryptophan within SST14 and the N-terminus of Aβ1-42...
June 26, 2017: ELife
https://www.readbyqxmd.com/read/28650232/pharmacokinetics-of-cyclophosphamide-and-4-hydroxycyclophosphamide-in-cats-after-oral-intravenous-and-intraperitoneal-administration-of-cyclophosphamide
#15
Katherine A Stroda, Jacqueline D Murphy, Ryan J Hansen, Lisa Brownlee, Elizabeth A Atencio, Daniel L Gustafson, Susan E Lana
OBJECTIVE To characterize pharmacokinetics of cyclophosphamide and 4-hydoxycyclophosphamide (4-OHCP) in the plasma of healthy cats after oral, IV, and IP administration of cyclophosphamide. ANIMALS 6 healthy adult cats. PROCEDURES Cats were randomly assigned to receive cyclophosphamide (200 mg/m(2)) via each of 3 routes of administration (oral, IV, and IP); there was a 30-day washout period between successive treatments. Plasma samples were obtained at various time points for up to 8 hours after administration...
July 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28650205/investigation-of-the-hepatoprotective-effect-of-prunus-mume-sieb-et-zucc-extract-in-a-mouse-model-of-alcoholic-liver-injury-through-high-resolution-metabolomics
#16
Adnan Khan, Jeong Hoon Pan, Seongha Cho, Sojung Lee, Young Jun Kim, Youngja H Park
This study aimed to identify the changes in the metabolomics profile of liver damage caused by alcohol consumption and verify beneficial effect of Prunus mume Sieb. et Zucc extract (PME) in protection of alcohol-induced injury by attenuating the level of identified metabolites. Mice were treated with PME and saline or untreated once daily for 5 days, followed by alcohol injection. The plasma samples were analyzed using liquid chromatography-mass spectrometry-based high-resolution metabolomics followed by a multivariate statistical analysis using MetaboAnalyst 3...
June 26, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28650171/comprehensive-lipoprotein-characterization-using-lipidomics-analysis-of-human-plasma
#17
Nicolas Christinat, Mojgan Masoodi
Lipoproteins are responsible for the transport of lipids and other nutrients in the circulation and therefore play an important role in lipid metabolism and dyslipidemia. They have also been linked to multiple metabolic disorders including cardiovascular disease, thus understanding their lipid composition is of crucial importance. Characterization of lipoproteins is a challenging task due to their heterogeneity. In particular, their fractionation is often laborious and time-consuming, making large sets of clinical samples difficult to analyze...
June 26, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28650156/discovery-of-a-glutamine-kinase-required-for-the-biosynthesis-of-the-o-methyl-phosphoramidate-modifications-found-in-the-capsular-polysaccharides-of-campylobacter-jejuni
#18
Zane W Taylor, Haley A Brown, Tamari Narindoshvili, Cory Q Wenzel, Christine Szymanski, Hazel M Holden, Frank M Raushel
Bacterial capsular polysaccharides (CPS) are complex carbohydrate structures that play a role in the overall fitness of the organism. Campylobacter jejuni, known for being a major cause of bacterial gastroenteritis worldwide, produces a CPS with a unique O-methyl phosphoramidate (MeOPN) modification on specific sugar residues. The formation of P-N bonds in nature is relatively rare and the pathway for the assembly of the phosphoramidate moiety in the CPS of C. jejuni is unknown. In this investigation we discovered that the initial transformation in the biosynthetic pathway for the MeOPN modification of the CPS involves the direct phosphorylation of the amide nitrogen of L-glutamine with ATP by the catalytic activity of Cj1418...
June 26, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28650151/orthogonal-comparison-of-gc-ms-and-1-h-nmr-spectroscopy-for-short-chain-fatty-acid-quantitation
#19
Jingwei Cai, Jingtao Zhang, Yuan Tian, Limin Zhang, Emmanuel Hatzakis, Kristopher W Krausz, Philip B Smith, Frank J Gonzalez, Andrew D Patterson
Short chain fatty acids (SCFAs) are important regulators of host physiology and metabolism and may contribute to obesity and associated metabolic diseases. Interest in SCFAs has increased in part due to the recognized importance of how production of SCFAs by the microbiota may signal to the host. Therefore, reliable, reproducible, and affordable methods for SCFA profiling are required for accurate identification and quantitation. In the current study, four different methods for SCFA (acetic acid, propionic acid, and butyric acid) extraction and quantitation were compared using two independent platforms including gas chromatography coupled with mass spectrometry (GC-MS) and (1)H nuclear magnetic resonance (NMR) spectroscopy...
June 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28650149/rplc-hilic-and-sfc-with-mass-spectrometry-polarity-extended-organic-molecule-screening-in-environmental-water-samples
#20
Stefan Bieber, Giorgia Greco, Sylvia Grosse, Thomas Letzel
Trace organic compounds are important in environmental analysis, because they impact water quality and introduce potential (eco)toxicological effects. Current analytical methods mostly rely on gas chromatography (GC) or reversed-phase liquid chromatography (RPLC) coupled with (tandem) mass spectrometry. However, neither method can easily separate very polar molecules. This study presents, two chromatographic separation strategies, a serial RPLC- hydrophilic interaction liquid chromatography (RPLC-HILIC) coupling and an analytical scale supercritical fluid chromatography (SFC) system, and validates their separation effectiveness as polarity-extended chromatographic methods for 274 environmentally relevant compounds...
June 26, 2017: Analytical Chemistry
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