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https://www.readbyqxmd.com/read/28922898/brewer-s-yeast-saccharomyces-cerevisiae-enhances-attraction-of-two-invasive-yellowjackets-hymenoptera-vespidae-to-dried-fruit-and-fruit-powder
#1
Tamara Babcock, Regine Gries, John Borden, Luis Palmero, Analía Mattiacci, Maité Masciocchi, Juan Corley, Gerhard Gries
The German yellowjacket, Vespula germanica F., and common yellowjacket, Vespula vulgaris L. (Hymenoptera: Vespidae), are pests of significant economic, environmental, and medical importance in many countries. There is a need for the development and improvement of attractive baits that can be deployed in traps to capture and kill these wasps in areas where they are a problem. Yellowjackets are known to feed on fermenting fruit, but this resource is seldom considered as a bait due to its ephemeral nature and its potential attractiveness to nontarget species...
September 1, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28922661/lysophosphatidic-acid-inhibits-insulin-signaling-in-primary-rat-hepatocytes-via-the-lpa3-receptor-subtype-and-is-increased-in-obesity
#2
Susann Fayyaz, Lukasz Japtok, Fabian Schumacher, Dominik Wigger, Tim Julius Schulz, Kathrin Haubold, Erich Gulbins, Heinz Völler, Burkhard Kleuser
BACKGROUND/AIMS: Obesity is a main risk factor for the development of hepatic insulin resistance and it is accompanied by adipocyte hypertrophy and an elevated expression of different adipokines such as autotaxin (ATX). ATX converts lysophosphatidylcholine to lysophosphatidic acid (LPA) and acts as the main producer of extracellular LPA. This bioactive lipid regulates a broad range of physiological and pathological responses by activation of LPA receptors (LPA1-6). METHODS: The activation of phosphatidylinositide 3-kinases (PI3K) signaling (Akt and GSK-3ß) was analyzed via western blotting in primary rat hepatocytes...
September 1, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28922649/determination-of-74-new-psychoactive-substances-in-serum-using-automated-in-line-solid-phase-extraction-liquid-chromatography-tandem-mass-spectrometry
#3
Sabrina Lehmann, Tobias Kieliba, Justus Beike, Mario Thevis, Katja Mercer-Chalmers-Bender
A detailed description is given of the development and validation of a fully automated in-line solid-phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method capable of detecting 90 central-stimulating new psychoactive substances (NPS) and 5 conventional amphetamine-type stimulants (amphetamine, 3,4-methylenedioxy-methamphetamine (MDMA), 3,4-methylenedioxy-amphetamine (MDA), 3,4-methylenedioxy-N-ethyl-amphetamine (MDEA), methamphetamine) in serum. The aim was to apply the validated method to forensic samples...
September 7, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28922609/serum-proteomic-variability-associated-with-clinical-phenotype-in-familial-transthyretin-amyloidosis-attrm
#4
Gloria G Chan, Clarissa M Koch, Lawreen H Connors
Transthyretin (TTR), normally a plasma circulating protein, can become misfolded and aggregated, ultimately leading to extracellular deposition of amyloid fibrils usually targeted to heart or nerve tissues. Referred to as TTR-associated amyloidoses (ATTR), this group of diseases is frequently life threatening and fatal if untreated. ATTR, caused by amyloid-forming variant TTR proteins (ATTRm) which arise from point mutations in the TTR gene, were classically referred to as familial amyloid cardiomyopathy (FAC) or familial amyloid polyneuropathy (FAP) reflecting the clinical phenotype...
September 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28922607/antdas-automatic-data-analysis-strategy-for-uplc-qtof-based-nontargeted-metabolic-profiling-analysis
#5
Hai-Yan Fu, Xiao-Ming Guo, Yue-Ming Zhang, Jing-Jing Song, Qing-Xia Zheng, Ping-Ping Liu, Peng Lu, Qian-Si Chen, Yong-Jie Yu, Yuanbin She
High-quality data analysis methodology remains a bottleneck for metabolic profiling analysis based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. The present work aims to address this problem by proposing a novel data analysis strategy wherein (1) chromatographic peaks in the UPLC-QTOF dataset are automatically extracted by using an advanced multiscale Gaussian smoothing-based peak extraction strategy. (2) A peak annotation stage is used to cluster fragment ions that belong to the same compound...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28922593/cysteine-silac-mass-spectrometry-enables-the-identification-and-quantitation-of-scrambled-inter-chain-disulfide-bonds-preservation-of-native-heavy-light-chain-pairing-in-bispecific-iggs-generated-by-controlled-fab-arm-exchange
#6
Ewald T J van den Bremer, Aran F Labrijn, Ramon van den Boogaard, Patrick Priem, Kai Scheffler, Joost P M Melis, Janine Schuurman, Paul Parren, Rob N de Jong
Bispecific antibodies (bsAbs) are one of the most versatile and promising pharmaceutical innovations for countering heterogeneous and refractory disease by virtue of their ability to bind two distinct antigens. One critical quality attribute of bsAb formation requiring investigation is the potential randomization of cognate heavy chain (H)/light (L) chain pairing, which could occur to a varying extent dependent on bsAb format and the production platform. To assess the content of such HL chain-swapped reaction products with high sensitivity, we developed cysteine-SILAC, a method that facilitates the detailed characterization of disulfide-bridged peptides by mass-spectrometry...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28922569/strategies-for-analysis-of-isomeric-peptides
#7
Erik T Jansson
This review presents an overview and recent progress of strategies for detecting isomerism in peptides, with focus on d/l epimerization and the various isomers that the presence of an aspartic acid residue may yield in a protein or peptide. While mass spectrometry has become a majorly used method of choice within proteomics, isomerism is inherently difficult to analyze because it is a modification that does not yield any change in mass of the analyte. Here, several techniques used for analysis of peptide isomerism are discussed, including enzymatic assays, liquid chromatography and capillary electrophoresis...
September 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28922568/spectral-libraries-for-swath-ms-assays-for-drosophila-melanogaster-and-solanum-lycopersicum
#8
Bertrand Fabre, Dagmara Korona, Clara I Mata-Martinez, Harriet T Parsons, Michael J Deery, Maarten L A T M Hertog, Bart M Nicolaï, Steven Russell, Kathryn S Lilley
Quantitative proteomics methods have emerged as powerful tools for measuring protein expression changes at the proteome level. Using mass-spectrometry (MS) based approaches, it is now possible to routinely quantify thousands of proteins. However, pre-fractionation of the samples at the protein or peptide level is usually necessary to go deep into the proteome, increasing both MS analysis time and technical variability. Recently, a new MS acquisition method named SWATH was introduced with the potential to provide good coverage of the proteome as well as a good measurement precision without prior sample fractionation...
September 18, 2017: Proteomics
https://www.readbyqxmd.com/read/28922554/performance-comparison-of-dispersive-solid-phase-extraction-and-multiplug-filtration-cleanup-methods-for-the-determination-of-tefuryltrione-in-plant-and-environmental-samples-using-uhplc-ms-ms
#9
Yang Yu, Yan Tao, Fengshou Dong, Jun Xu, Xingang Liu, Xiaohu Wu, Mingshan Ji, Yongquan Zheng
The detection frequencies of tefuryltrione, a new type of 4-hydroxyphenyl-pyruvate dioxygenase inhibitor herbicide, are rarely reported, probably because of the paucity of analytical methods. Herein, an effective and sensitive analytical method has been developed to detect tefuryltrione in vegetables (tomato and cucumber), cereals (rice and corn), soil and water by ultra high performance liquid chromatography coupled with tandem mass spectrometry. Comparisons of the performances of dispersive solid-phase extraction and multiplug filtration cleanup methods were carried out for tefuryltrione in complex matrices...
September 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28922548/lncrna-plac2-down-regulates-rpl36-expression-and-blocks-cell-cycle-progression-in-glioma-through-a-mechanism-involving-stat1
#10
Yan-Wei Hu, Chun-Min Kang, Jing-Jing Zhao, Ying Nie, Lei Zheng, Hai-Xia Li, Xin Li, Qian Wang, Yu-Rong Qiu
Current glioma therapies allow in situ delivery of cytotoxic drugs to the tumour; however, gliomas show early recurrence due to their highly proliferative character. Long non-coding (lnc)RNAs play critical roles in tumorigenesis by controlling cell proliferation and cycling. However, the mechanism of action of lncRNAs in glioma development remains unclear. Here, we report that the lncRNA PLAC2 induces cell cycle arrest by targeting ribosomal protein (RP)L36 in glioma. RPL36 promoted cell proliferation and G1/S cell cycle progression...
September 18, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28922481/photo-induced-assembly-of-a-luminescent-tetraruthenium-square
#11
Baptiste Laramée-Milette, Francesco Nastasi, Fausto Puntoriero, Sebastiano Campagna, Garry S Hanan
Self-assembly is a powerful synthetic tool that has led to the development of one-, two- and three-dimensional architectures. From MOFs to molecular flasks, self-assembled materials have proven to be of great interest to the scientific community. Here we describe a strategy for the construction and de-construction of a supramolecular structure through unprecedented photo-induced assembly and dis-assembly. The combination of two approaches, a [n×1]-directional bonding strategy and a ligand photo-dissociation strategy, allows the photo-induced assembly of a polypyridyl Ru(II) precursor into a discrete molecular square...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28922472/mirna-506-promotes-primary-biliary-cholangitis-like-features-in-cholangiocytes-and-immune-activation
#12
Oihane Erice, Patricia Munoz-Garrido, Javier Vaquero, Maria J Perugorria, Maite G Fernandez-Barrena, Elena Saez, Alvaro Santos-Laso, Ander Arbelaiz, Raul Jimenez-Agüero, Joaquin Fernandez-Irigoyen, Enrique Santamaria, Verónica Torrano, Arkaitz Carracedo, Meenakshisundaram Ananthanarayanan, Marco Marzioni, Jesus Prieto, Ulrich Beuers, Ronald P Oude Elferink, Nicholas F LaRusso, Luis Bujanda, Jose J G Marin, Jesus M Banales
Primary biliary cholangitis (PBC) is a chronic cholestatic liver disease associated with autoimmune phenomena targeting intrahepatic bile duct cells (cholangiocytes). Although PBC etiopathogenesis still remains obscure, development of anti-mitochondrial auto-antibodies against pyruvate dehydrogenase complex-E2 (PDC-E2) is a common feature. MicroRNA (miR) dysregulation occurs in liver and immune cells of PBC patients, but their functional relevance is largely unknown. We previously reported that miR-506 is overexpressed in PBC cholangiocytes and directly targets both Cl(-) /HCO3(-) anion exchanger 2 (AE2) and type III inositol 1,4,5-trisphosphate receptor (InsP3R3), leading to cholestasis...
September 18, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28922398/role-of-the-p2-residue-of-human-alpha-1-antitrypsin-in-determining-target-protease-specificity
#13
Hye-Shin Chung, Ji-Sun Kim, Sang Mee Lee, Soon Jae Park
Alpha 1-antitrypsin (A1AT) is a serine protease inhibitor that mainly inhibits neutrophil elastase in the lungs. A variant of A1AT at the P1 position with methionine 358 to arginine (A1AT-Pittsburgh) is a rapid inhibitor of thrombin with greatly diminished anti-elastase activity. The P2 residue (position 357) of A1AT-Pittsburgh has been shown to play an important role in interactions with thrombin and kallikrein, but the role of P2 residue in wild-type A1AT has largely been unraveled. Here, we investigated the effects of P2 proline substitutions in wild-type A1AT on interactions with porcine pancreatic elastase (PPE) and human neutrophil elastase (HNE)...
2017: PloS One
https://www.readbyqxmd.com/read/28922389/comprehensive-metabolic-characterization-of-serum-osteocalcin-action-in-a-large-non-diabetic-sample
#14
Lukas Entenmann, Maik Pietzner, Anna Artati, Anke Hannemann, Ann-Kristin Henning, Gabi Kastenmüller, Henry Völzke, Matthias Nauck, Jerzy Adamski, Henri Wallaschofski, Nele Friedrich
Recent research suggested a metabolic implication of osteocalcin (OCN) in e.g. insulin sensitivity or steroid production. We used an untargeted metabolomics approach by analyzing plasma and urine samples of 931 participants using mass spectrometry to reveal further metabolic actions of OCN. Several detected relations between OCN and metabolites were strongly linked to renal function, however, a number of associations remained significant after adjustment for renal function. Intermediates of proline catabolism were associated with OCN reflecting the implication in bone metabolism...
2017: PloS One
https://www.readbyqxmd.com/read/28922385/metabolomics-analyses-identify-platelet-activating-factors-and-heme-breakdown-products-as-lassa-fever-biomarkers
#15
Trevor V Gale, Timothy M Horton, Donald S Grant, Robert F Garry
Lassa fever afflicts tens of thousands of people in West Africa annually. The rapid progression of patients from febrile illness to fulminant syndrome and death provides incentive for development of clinical prognostic markers that can guide case management. The small molecule profile of serum from febrile patients triaged to the Viral Hemorrhagic Fever Ward at Kenema Government Hospital in Sierra Leone was assessed using untargeted Ultra High Performance Liquid Chromatography Mass Spectrometry. Physiological dysregulation resulting from Lassa virus (LASV) infection occurs at the small molecule level...
September 18, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28921992/a-mechanistic-understanding-of-the-synergistic-potential-of-azole-fungicides-in-the-aquatic-invertebrate-gammarus-pulex
#16
Andrea Rösch, Michele Gottardi, Caroline Vignet, Nina Cedergreen, Juliane Hollender
Azole fungicides are known inhibitors of the important enzyme class cytochrome P450 monooxygenases (CYPs), thereby influencing the detoxification of co-occurring substances via biotransformation. This synergism in mixtures containing an azole has mostly been studied by effect measurements, while the underlying mechanism has been less well investigated. In this study, six azole fungicides (cyproconazole, epoxiconazole, ketoconazole, prochloraz, propiconazole and tebuconazole) were selected to investigate their synergistic potential and their CYP inhibition strength in the aquatic invertebrate Gammarus pulex...
September 18, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28921983/the-first-state-in-the-catalytic-cycle-of-the-water-oxidizing-enzyme-identification-of-a-water-derived-%C3%AE-hydroxo-bridge
#17
Thomas Lohmiller, Vera Krewald, Arezki Sedoud, A William Rutherford, Frank Neese, Wolfgang Lubitz, Dimitrios A Pantazis, Nicholas Cox
Nature's water splitting catalyst, an oxygen-bridged tetra-manganese calcium (Mn4O5Ca) complex, sequentially activates two substrate water molecules generating molecular O2. Its reaction cycle is composed of five intermediate (Si) states, where the index i indicates the number of oxidizing equivalents stored by the cofactor. After formation of the S4 state, the product dioxygen is released and the cofactor returns to its lowest oxidation state, S0. Membrane-inlet mass spectrometry measurements suggest that at least one substrate is bound throughout the catalytic cycle, as the rate of (18)O-labeled water incorporation into the product O2 is slow, on a millisecond to second timescale depending on the S state...
September 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28921982/mortiamides-a-d-cyclic-heptapeptides-from-a-novel-mortierella-sp-obtained-from-frobisher-bay
#18
Alyssa L Grunwald, Fabrice Berrue, Andrew W Robertson, David P Overy, Russell G Kerr
Four new cyclic heptapeptides, mortiamides A-D (1-4), were obtained from a novel Mortierella sp. isolate obtained from marine sediment collected from the intertidal zone of Frobisher Bay, Nunavut, Canada. The structures of the compounds were elucidated by NMR spectroscopy and tandem mass spectrometry. The absolute configurations of the amino acids were determined using Marfey's method. Localization of l and d amino acids within each compound was ascertained by retention time comparison of the partial hydrosylate products of each compound to synthesized dipeptide standards using LC-HRMS...
September 18, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28921966/tool-for-rapid-analysis-of-glycopeptide-by-permethylation-trap-via-one-pot-site-mapping-and-glycan-analysis
#19
Asif Shajahan, Nitin Tatyaso Supekar, Christian Heiss, Mayumi Ishihara, Parastoo Azadi
To overcome the challenges in the analysis of protein glycosylation, we have developed a comprehensive and universal tool through permethylation of glycopeptides and their tandem mass spectrometric analysis. This method has the potential to simplify glycoprotein analysis by integrating glycan sequencing and glycopeptide analysis in a single experiment. Moreover, glycans with unique glycosidic linkages, particularly from prokaryotes, which are resistant to enzymatic or chemical release, could also be detected and analyzed by this methodology...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28921887/chemical-components-biological-activities-and-toxicological-evaluation-of-the-fruit-aril-of-two-precious-plant-species-from-genus-taxus
#20
Na Li, Zheng Pan, Dan Zhang, Huaxing Wang, Bao Yu, Shengping Zhao, Jiajia Guo, Jianwei Wang, Ling Yao, Weiguo Cao
The fruit (aril) of the endangered genus Taxus plants is an abandoned herbal resource. Traditionally, people enthusiasticly focus on its bark, its renewable, tremendous arils fall into the soil with seeds after they are mature. The present research investigated the fruit of two species from the genus Taxus, Taxus chinensis var. mairei and Taxus media, with regards to their antioxidant and antihyperglycaemic activities, safety, and bioactive constituents. Results showed that T. chinensis var. mairei and T. media both had certain biological activities with T...
September 18, 2017: Chemistry & Biodiversity
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