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Cecropin

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https://www.readbyqxmd.com/read/28421044/comparative-genomics-of-glossina-palpalis-gambiensis-and-g-morsitans-morsitans-to-reveal-gene-orthologs-involved-in-infection-by-trypanosoma-brucei-gambiense
#1
Illiassou Hamidou Soumana, Bernadette Tchicaya, Stéphanie Rialle, Hugues Parrinello, Anne Geiger
Blood-feeding Glossina palpalis gambiense (Gpg) fly transmits the single-celled eukaryotic parasite Trypanosoma brucei gambiense (Tbg), the second Glossina fly African trypanosome pair being Glossina morsitans/T.brucei rhodesiense. Whatever the T. brucei subspecies, whereas the onset of their developmental program in the zoo-anthropophilic blood feeding flies does unfold in the fly midgut, its completion is taking place in the fly salivary gland where does emerge a low size metacyclic trypomastigote population displaying features that account for its establishment in mammals-human individuals included...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28411220/the-global-transcription-factor-lrp-is-both-essential-for-and-inhibitory-to-xenorhabdus-nematophila-insecticidal-activity
#2
Ángel M Casanova-Torres, Upasana Shokal, Neta Morag, Ioannis Eleftherianos, Heidi Goodrich-Blair
In the entomopathogenic bacterium Xenorhabdus nematophila, cell-to-cell variation in the abundance of the Lrp transcription factor leads to virulence modulation: low Lrp levels are associated with a virulent phenotype and suppression of antimicrobial peptides (AMPs) in Manduca sexta insects, while cells that lack lrp or express high Lrp levels are virulence attenuated and elicit AMP expression. To better understand the basis of these phenotypes we examined X. nematophila expressing fixed Lrp levels. Unlike the lrp null mutant, the high-lrp strain is fully virulent in Drosophila melanogaster suggesting that these two strains have distinct underlying causes of virulence attenuation in M...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28395586/differential-change-patterns-of-main-antimicrobial-peptide-genes-during-infection-of-entompathogenic-nematode-and-their-symbiotic-bacteria
#3
Reyhaneh Darsouei, Javad Karimi, Mohammad Ghadamyari, Mojtaba Hosseini
The expression of antimicrobial peptides (AMPs) as main humoral defense reactions of insects during infection by entomopathogenic nematodes (EPNs) and their symbiont is addressed herein. Three AMPs, attacin, cecropin, and spodoptericin, were evaluated in the fifth instar larvae of Spodoptera exigua Hübner when challenged with Steinernema carpocapsae or Heterorhabditis bacteriophora. The results indicated that attacin was expressed to a greater extent than either cecropin or spodoptericin. While spodoptericin was expressed to a much lesser extent, this AMP was induced against gram-positive bacteria, thus not expressed after penetration of Xenorhabdus nematophila and Photorhabdus luminescens...
April 10, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28347740/selective-cytotoxicity-of-the-antibacterial-peptide-abp-dhc-cecropin-a-and-its-analog-towards-leukemia-cells
#4
Ming Sang, Jiaxin Zhang, Qiang Zhuge
Some cationic antibacterial peptides, with typical amphiphilic α-helical conformations in a membrane-mimicking environment, exhibit anticancer properties as a result of a similar mechanism of action towards both bacteria and cancer cells. We previously reported the cDNA sequence of the antimicrobial peptide ABP-dHC-Cecropin A precursor cloned from drury (Hyphantria cunea) (dHC). In the present study, we synthesized and structurally characterized ABP-dHC-Cecropin A and its analog, ABP-dHC-Cecropin A-K(24). Circular dichroism spectroscopy showed that ABP-dHC-Cecropin A and its analog adopt a well-defined α-helical structure in a 50% trifluorethanol solution...
March 27, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28344121/brevibacillus-laterosporus-pathogenesis-and-local-immune-response-regulation-in-the-house-fly-midgut
#5
Maria Elena Mura, Luca Ruiu
The insect midgut represents the primary site of action of the entompathogenic bacterium Brevibacillus laterosporus. While most studies on this microorganism focus on the identification and characterization of possible virulence factors and toxins, little is known about the insect immune defense mechanisms that are activated against this pathogen. In this study we have investigated the local immune response of different house fly stages to B. laterosporus at the transcriptional level, and we tested the hypothesis that an improvement in entomopathogenicity can be achieved by impairing host innate immunity...
March 23, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28334331/alterations-of-immune-parameters-on-trichoplusia-ni-lepidoptera-noctuidae-larvae-exposed-to-extremely-low-frequency-electromagnetic-fields
#6
Jose A Valadez-Lira, Nahui O Medina-Chavez, Alonso A Orozco-Flores, J Antonio Heredia-Rojas, Abraham O Rodriguez-de la Fuente, Ricardo Gomez-Flores, Juan M Alcocer-Gonzalez, Patricia Tamez-Guerra
Worldwide mobile telephone and microwave use have resulted in an increasing presence of extremely low-frequency electromagnetic field radiations (ELF-EMFs) in ecosystems. ELF-EMFs have been associated with altered physiological processes that can adversely affect exposed organisms. In this study, Trichoplusia ni Hübner larvae were exposed for 24, 48, or 72 h to ELF-EMFs (60 Hz and 2.0 mT) to assess effects on immune response parameters and fertility. Trichoplusia ni life cycle and fertility were not affected by 24-h exposure...
April 1, 2017: Environmental Entomology
https://www.readbyqxmd.com/read/28326334/bactericide-immunomodulating-and-wound-healing-properties-of-transgenic-kalanchoe-pinnata-synergize-with-antimicrobial-peptide-cecropin-p1-in-vivo
#7
COMPARATIVE STUDY
A A Lebedeva, N S Zakharchenko, E V Trubnikova, O A Medvedeva, T V Kuznetsova, G A Masgutova, M V Zylkova, Y I Buryanov, A S Belous
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28278280/natural-antimicrobial-peptide-complexes-in-the-fighting-of-antibiotic-resistant-biofilms-calliphora-vicina-medicinal-maggots
#8
Natalia Gordya, Andrey Yakovlev, Anastasia Kruglikova, Dmitry Tulin, Evdokia Potolitsina, Tatyana Suborova, Domenico Bordo, Camillo Rosano, Sergey Chernysh
Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast majority of human bacterial infections and many severe complications such as chronic inflammatory diseases and cancer. Biofilms' resistance to the host immunity and antibiotics makes this kind of infection particularly intractable. Antimicrobial peptides (AMPs) are a ubiquitous facet of innate immunity in animals. However, AMPs activity was studied mainly on planktonic bacteria and little is known about their effects on biofilms...
2017: PloS One
https://www.readbyqxmd.com/read/28258224/transglutaminase-catalyzed-incorporation-of-polyamines-masks-the-dna-binding-region-of-the-transcription-factor-relish
#9
Kouki Maki, Toshio Shibata, Shun-Ichiro Kawabata
In Drosophila, the final immune deficiency (IMD) pathway-dependent signal is transmitted through proteolytic conversion of the nuclear factor-κB (NF-κB)-like transcription factor Relish to the active N-terminal fragment Relish-N. Relish-N is then translocated from the cytosol into the nucleus for the expression of IMD-controlled genes. We previously demonstrated that transglutaminase (TG) suppresses the IMD pathway by polymerizing Relish-N to inhibit its nuclear translocation. Conversely, we also demonstrated that orally ingested synthetic amines, such as monodansylcadaverine (DCA) and biotin-labeled pentylamine, are TG-dependently incorporated into Relish-N, causing the nuclear translocation of modified Relish-N in gut epithelial cells...
April 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28228017/adsorption-mechanism-of-an-antimicrobial-peptide-on-carbonaceous-surfaces-a-molecular-dynamics-study
#10
Danilo Roccatano, Edita Sarukhanyan, Ronen Zangi
Peptides are versatile molecules with applications spanning from biotechnology to nanomedicine. They exhibit a good capability to unbundle carbon nanotubes (CNT) by improving their solubility in water. Furthermore, they are a powerful drug delivery system since they can easily be uptaken by living cells, and their high surface-to-volume ratio facilitates the adsorption of molecules of different natures. Therefore, understanding the interaction mechanism between peptides and CNT is important for designing novel therapeutical agents...
February 21, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28194425/molecular-cloning-bioinformatic-analysis-and-expression-of-bombyx-mori-lebocin-5-gene-related-to-beauveria-bassiana-infection
#11
Dingding Lü, Chengxiang Hou, Guangxing Qin, Kun Gao, Tian Chen, Xijie Guo
A full-length cDNA of lebocin 5 (BmLeb5) was first cloned from silkworm, Bombyx mori, by rapid amplification of cDNA ends. The BmLeb5 gene is 808 bp in length and the open reading frame encodes a 179-amino acid hydroxyproline-rich peptide. Bioinformatic analysis results showed that BmLeb5 owns an O-glycosylation site and four RXXR motifs as other lebocins. Sequence similarity and phylogenic analysis results indicated that lebocins form a multiple gene family in silkworm as cecropins. Quantitative real-time PCR analysis revealed that BmLeb5 was highest expressed in the fat body...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28181522/corrigendum-efficient-biosynthesis-of-a-cecropin-a-melittin-mutant-in-bacillus-subtilis-wb700
#12
Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Hengxin Li, Binyun Cao, Hongfu Zhang
No abstract text is available yet for this article.
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178190/high-specific-selectivity-and-membrane-active-mechanism-of-synthetic-cationic-hybrid-antimicrobial-peptides-based-on-the-peptide-fv7
#13
Tingting Tan, Di Wu, Weizhong Li, Xin Zheng, Weifen Li, Anshan Shan
Hybrid peptides integrating different functional domains of peptides have many advantages, such as remarkable antimicrobial activity, lower hemolysis and ideal cell selectivity, compared with natural antimicrobial peptides. FV7 (FRIRVRV-NH₂), a consensus amphiphilic sequence was identified as being analogous to host defense peptides. In this study, we designed a series of hybrid peptides FV7-LL-37 (17-29) (FV-LL), FV7-magainin 2 (9-21) (FV-MA) and FV7-cecropin A (1-8) (FV-CE) by combining the FV7 sequence with the small functional sequences LL-37 (17-29) (LL), magainin 2 (9-21) (MA) and cecropin A (1-8) (CE) which all come from well-described natural peptides...
February 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28092440/molecular-interactions-between-graphene-and-biological-molecules
#14
Xingquan Zou, Shuai Wei, Joshua Jasensky, Minyu Xiao, Qiuming Wang, Charles L Brooks Iii, Zhan Chen
Applications of graphene have extended into areas of nanobio-technology such as nanobio-medicine, nanobio-sensing, as well as nanoelectronics with biomolecules. These applications involve interactions between proteins, peptides, DNA, RNA etc. and graphene, therefore understanding such molecular interactions is essential. For example, many applications based on using graphene and peptides require peptides to interact with (e.g., noncovalently bind to) graphene at one end, while simultaneously exposing the other end to the surrounding medium (e...
January 27, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28089733/antimicrobial-peptide-gene-cecropin-2-and-defensin-respond-to-peptidoglycan-infection-in-the-female-adult-of-oriental-fruit-fly-bactrocera-dorsalis-hendel
#15
Shi-Huo Liu, Dong Wei, Guo-Rui Yuan, Hong-Bo Jiang, Wei Dou, Jin-Jun Wang
Cecropins and defensins are important antimicrobial peptides in insects and are inducible after injection of immune triggers. In this study, we cloned the cDNAs of two antimicrobial peptides (AMPs), cecropin-2 (BdCec-2) and defensin (BdDef) from Bactrocera dorsalis (Hendel), a serious pest causing great economic losses to fruits and vegetables. The BdCec-2 sequence of 192bp encodes a protein of 63 amino acids residues with a predicted molecular weight of 6.78kD. The 282bp cDNA of BdDef encodes a protein of 93 residues with a predicted molecular weight of 9...
April 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28071737/efficient-biosynthesis-of-a-cecropin-a-melittin-mutant-in-bacillus-subtilis-wb700
#16
Shengyue Ji, Weili Li, Abdul Rasheed Baloch, Meng Wang, Hengxin Li, Binyun Cao, Hongfu Zhang
The efficient production of antimicrobial peptides (AMPs) for clinical applications has attracted the attention of the scientific community. To develop a novel microbial cell factory for the efficient biosynthesis of a cecropin A-melittin mutant (CAM-W), a recombinant Bacillus subtilis WB700 expression system was genetically modified with a novel vector, including a fusion gene encoding CAM-W, the autoprotease EDDIE and the signal peptide SacB under the control of the maltose-inducible promoter Pglv. A total of 159 mg of CAM-W was obtained from 1 L of fermentation supernatant...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28013509/studying-the-interaction-of-magainin-2-and-cecropin-a-with-e-coli-bacterial-cells-using-circular-dichroism
#17
Concetta Avitabile, Luca Domenico D'Andrea, Alessandra Romanelli
The potential of antimicrobial peptides (AMPs) as an effective therapeutic alternative to classic and current antibiotics has encouraged studies to understand how they interact with the bacterial membrane. Here we describe how to detect, by circular dichroism (CD), the secondary structures of two antimicrobial peptides, magainin 2 and cecropin A, in the presence of E. coli bacterial cells.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28008655/characterization-and-functional-study-of-a-cecropin-like-peptide-from-the-chinese-oak-silkworm-antheraea-pernyi
#18
Shao-Liang Fang, Lei Wang, Qi Fang, Chen Chen, Xiao-San Zhao, Cen Qian, Guo-Qing Wei, Bao-Jian Zhu, Chao-Liang Liu
In present study, a Cecropin-like peptide from Antheraea pernyi (ApCec) was cloned and characterized. The full-length ApCec cDNA encoded a protein with 64 amino acids including a putative 22-amino-acid signal peptide, a 4-amino-acid propeptide, and a 38-amino-acid mature peptide. ApCec gene was highly expressed in Malpighian tubules of A. pernyi after induction for 24 h by Escherichia coli in PBS. Pro-ApCec (including propeptide and mature peptide) and M-ApCec (just mature peptide) were synthesized chemically and analyzed by HPLC and mass spectroscopy...
January 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/27999446/antimicrobial-activity-and-interactions-of-cationic-peptides-derived-from-galleria-mellonella-cecropin-d-like-peptide-with-model-membranes
#19
José Oñate-Garzón, Marcela Manrique-Moreno, Steven Trier, Chad Leidy, Rodrigo Torres, Edwin Patiño
Antimicrobial peptides are effector molecules of the innate immune system against invading pathogens. The cationic charge in their structures has a strong correlation with antimicrobial activity, being responsible for the initial electrostatic interaction between peptides and the anionic microbial surface. This paper contains evidence that charge modification in the neutral peptide Gm cecropin D-like (WT) improved the antimicrobial activity of the modified peptides. Two cationic peptides derived from WT sequence named as ΔM1 and ΔM2, with net charge of +5 and +9, respectively, showed at least an eightfold increase in their antimicrobial activity in comparison to WT...
March 2017: Journal of Antibiotics
https://www.readbyqxmd.com/read/27867076/the-dna-chaperone-hmgb1-potentiates-the-transcriptional-activity-of-rel1a-in-the-mosquito-aedes-aegypti
#20
Anderson de Mendonça Amarante, Natapong Jupatanakul, Isabel Caetano de Abreu da Silva, Vitor Coutinho Carneiro, Amanda Roberta Revoredo Vicentino, George Dimopolous, Octávio Augusto C Talyuli, Marcelo Rosado Fantappié
High Mobility Group protein 1 (HMGB1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. We investigated the influence and mechanism of action of Aedes aegypti HMGB1 (AaHMGB1) on mosquito Rel1A-mediated transcription from target gene promoters. The DNA-binding domain (RHD) of AaRel1A was bacterially expressed and purified, and AaHMGB1 dramatically enhanced RHD binding to consensus NF-kB/Rel DNA response elements. Luciferase reporter analyses using a cecropin gene promoter showed that AaHMGB1 potentiates the transcriptional activity of AaRel1A in Aag-2 cells...
January 2017: Insect Biochemistry and Molecular Biology
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