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https://www.readbyqxmd.com/read/29764917/the-path-to-victory-a-beginner-s-guide-to-success-using-commercial-research-antibodies
#1
REVIEW
Simon L Goodman
Commercial research antibodies are crucial tools in modern cell biology and biochemistry. In the USA some $2 billion a year are spent on them, but many are apparently not fit-for-purpose, and this may contribute to the 'reproducibility crisis' in biological sciences. Inadequate antibody validation and characterization, lack of user awareness, and occasional incompetence amongst suppliers have had immense scientific and personal costs. In this Opinion, I suggest some paths to make the use of these vital tools more successful...
May 15, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29603941/hyperspectral-database-of-fruits-and-vegetables
#2
Robert Ennis, Florian Schiller, Matteo Toscani, Karl R Gegenfurtner
We have built a hyperspectral database of 42 fruits and vegetables. Both the outside (skin) and inside of the objects were imaged. We used a Specim VNIR HS-CL-30-V8E-OEM mirror-scanning hyperspectral camera and took pictures at a spatial resolution of ∼57  px/deg by 800 pixels at a wavelength resolution of ∼1.12  nm. A stable, broadband illuminant was used. Images and software are freely available on our webserver (http://www.allpsych.uni-giessen.de/GHIFVD; pronounced "gift"). We performed two kinds of analyses on these images...
April 1, 2018: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/29580189/occupational-exposure-monitoring-data-collection-storage-and-use-among-state-based-and-private-workers-compensation-insurers
#3
Taylor M Shockey, Kelsey R Babik, Steven J Wurzelbacher, Libby L Moore, Michael S Bisesi
Despite substantial financial and personnel resources being devoted to occupational exposure monitoring (OEM) by employers, workers' compensation insurers, and other organizations, the United States (US) lacks comprehensive occupational exposure databases to use for research and surveillance activities. OEM data are necessary for determining the levels of workers' exposures; compliance with regulations; developing control measures; establishing worker exposure profiles; and improving preventive and responsive exposure surveillance and policy efforts...
March 26, 2018: Journal of Occupational and Environmental Hygiene
https://www.readbyqxmd.com/read/29569902/tuning-electrochemical-properties-of-li-rich-layered-oxide-cathodes-by-adjusting-co-ni-ratios-and-mechanism-investigation-using-in-situ-x-ray-diffraction-and-online-continuous-flow-differential-electrochemical-mass-spectrometry
#4
ShouYu Shen, YuHao Hong, FuChun Zhu, ZhenMing Cao, YuYang Li, FuSheng Ke, JingJing Fan, LiLi Zhou, LiNa Wu, Peng Dai, MingZhi Cai, Ling Huang, ZhiYou Zhou, JunTao Li, QiHui Wu, ShiGang Sun
Owing to high specific capacity of ∼250 mA h g-1 , lithium-rich layered oxide cathode materials (Li1+ x Niy Coz Mn(3- x-2 y-3 z)/4 O2 ) have been considered as one of the most promising candidates for the next-generation cathode materials of lithium ion batteries. However, the commercialization of this kind of cathode materials seriously restricted by voltage decay upon cycling though Li-rich materials with high cobalt content have been widely studied and show good capacity. This research successfully suppresses voltage decay upon cycling while maintaining high specific capacity with low Co/Ni ratio in Li-rich cathode materials...
April 4, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29508164/dependence-and-power-in-healthcare-equipment-supply-chains
#5
Jurriaan L de Jong, W C Benton
Most healthcare organizations (HCOs) engage Group Purchasing Organizations (GPOs) as an outsourcing strategy to secure their supplies and materials. When an HCO outsources the procurement function to a GPO, this GPO will directly interact with the HCO's supplier on the HCO's behalf. This study investigates how an HCO's dependence on a GPO affects supply chain relationships and power in the healthcare medical equipment supply chain. Hypotheses are tested through factor analysis and structural equation modeling, using primary survey data from HCO procurement managers...
March 5, 2018: Health Care Management Science
https://www.readbyqxmd.com/read/29375088/minimizing-an-outbreak-of-avian-botulism-clostridium-botulinum-type-c-in-incheon-south-korea
#6
Kidong Son, Yong Kwan Kim, Chanjin Woo, Seung-Jun Wang, Youngsik Kim, Jae-Ku Oem, Weonhwa Jheong, Jipseol Jeong
An outbreak of botulism occurred over a two-month period beginning July 20, 2016. In all, 697 wild birds were found paralyzed or dead at the Namdong reservoir and 11 Gong-gu. Using a mouse bioassay, type C botulinum toxin was identified in the bird serum, liquid cultures of soil samples, and maggot extracts. To minimize further infection of wild birds, we opened the floodgates of the Namdong reservoir adjacent to the Yellow Sea; this decreased the water temperature and the nutrient load such as nitrogen and phosphorus...
March 30, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29339626/estimation-of-the-dilution-field-near-a-marine-outfall-by-using-effluent-turbidity-as-an-environmental-tracer-and-comparison-with-dye-tracer-data
#7
José Otavio Goulart Pecly
The alternative use of effluent turbidity to determine the dilution field of a domestic marine outfall located off the city of Rio de Janeiro was evaluated through field work comprising fluorescent dye tracer injection and tracking with simultaneous monitoring of sea water turbidity. A preliminary laboratory assessment was carried out with a sample of the outfall effluent whose turbidity was measured by the nephelometric method before and during a serial dilution process. During the field campaign, the dye tracer was monitored with field fluorometers and the turbidity was observed with an optical backscattering sensor interfaced to an OEM data acquisition system...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29230228/overexpression-of-smmyc2-increases-the-production-of-phenolic-acids-in-salvia-miltiorrhiza
#8
Na Yang, Wenping Zhou, Jiao Su, Xiaofan Wang, Lin Li, Liru Wang, Xiaoyan Cao, Zhezhi Wang
MYC2 is a core transcription factor in the plant response to jasmonates. It also functions in secondary metabolism and various processes for growth and development. However, the knowledge about its role in Salvia miltiorrhiza is still very limited. We determined that the biosynthesis of salvianolic acid B (Sal B) was strongly induced in 2-month-old transgenic plants that over-expressed SmMYC2 . In the roots of transgenic line 12 that over-expressed SmMYC2 (OEM-12), the Sal B concentration was as high as 5.95 ± 0...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29228260/molecular-detection-of-enterocytozoon-bieneusi-from-bats-in-south-korea
#9
Seung-Hun Lee, Jae-Ku Oem, Sae-Mi Lee, Kidong Son, Seong-Deok Jo, Dongmi Kwak
Enterocytozoon bieneusi, which has recently been re-classified as a fungus, was identified in 5.2% (3/58) bat intestinal tissues and 1.9% (4/210) bat feces collected in South Korea. The positive cases were classified into six genotypes including four novel genotypes, KBAT1-KBAT4, based on sequence analysis of the E. bieneusi internal transcribed spacer (ITS) region. In addition, a novel genotype, KBAT3, belonged to group 1, which is considered having zoonotic potential by phylogenetic analysis of the E. bieneusi ITS region...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29208530/analytical-performances-of-simultaneous-detection-of-hiv-1-hiv-2-and-hepatitis-c-specific-antibodies-and-hepatitis-b-surface-antigen-hbsag-by-multiplex-immunochromatographic-rapid-test-with-serum-samples-a-cross-sectional-study
#10
Leman Robin, Ralph-Sydney Mboumba Bouassa, Zita Aleyo Nodjikouambaye, Laura Charmant, Mathieu Matta, Sylvie Simon, Mounir Filali, Souleymane Mboup, Laurent Bélec
BACKGROUND: The HIV/HCV/HBsAg Triplex consists in manually performed, visually interpreted, lateral flow, immunochromatographic rapid diagnostic test simultaneously detecting in 15min human immunodeficiency virus (HIV)-1 and HIV-2 and hepatitis C virus (HCV)- specific antibodies (Ab) (IgG and IgM) and hepatitis B virus (HBV) surface antigen (HBsAg) in serum, plasma and whole blood. METHODS: A hospital-based cross-sectional study was conducted on a prospective panel of serum samples from adult inpatients included from routine analysis irrespectively of age and sex, including 250 sera positive for HIV-1-specific Ab, 250 for HCV-specific Ab, 250 for HBsAg and 250 sera negative for HIV- and HCV- Ab and HBsAg, and from 110 HIV-2-infected patients living in Ivory Coast, according to the results obtained by the reference chemiluminiscent microparticle immunoassay (CMIA) Abbott Architect i2000SR analyzer (Abbott Diagnostic, Chicago, IL, USA)...
March 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29169228/control-of-type-o-foot-and-mouth-disease-by-vaccination-in-south-korea-2014-2015
#11
Jong-Hyeon Park, Dongseob Tark, Kwang-Nyeong Lee, Ji-Eun Chun, Hyang-Sim Lee, Young-Joon Ko, Soo-Jeong Kye, Yong-Joo Kim, Jae-Ku Oem, Soyoon Ryoo, Sung-Bin Lim, Seo-Yong Lee, Joo-Hyung Choi, Mi-Kyeong Ko, Su-Hwa You, Myoung-Heon Lee, Byounghan Kim
On December 3, 2014, a type O foot-and-mouth disease (FMD) outbreak occurred in South Korea. Although vaccinations were administered, cases of FMD increased steadily for five months and reached 185 cases by April 2015. Most of the affected animals were pigs, which are vulnerable to vaccinations. The FMD virus belonged to the South-East Asia (SEA) topotype that had been observed three times in Korea from April 2010 to July 2014. However, the virus in December 2014 had a unique difference in a partial deletion of the 5' noncoding region, which had not been seen in the previous SEA topotype isolates that were identified in Korea...
November 23, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29155960/occupational-medicine-specialist-referral-triggers-mixed-methods-analysis-of-teleconsult-cases
#12
J L Eaton, A Mohammad, D C Mohr, D J Brustein, S R Kirkhorn
Background: Qualitative analyses can yield critical lessons for learning organizations in healthcare. Few studies have applied these techniques in the field of occupational and environmental medicine (OEM). Aims: To describe the characteristics of complex cases referred for OEM subspecialty evaluation and variation by referring provider's training. Methods: Using a mixed methods approach, we conducted a content analysis of clinical cases submitted to a national OEM teleconsult service...
December 30, 2017: Occupational Medicine
https://www.readbyqxmd.com/read/29060207/predictive-modeling-for-corrective-maintenance-of-imaging-devices-from-machine-logs
#13
Ravindra B Patil, Meru A Patil, Vidya Ravi, Sarif Naik
In the cost sensitive healthcare industry, an unplanned downtime of diagnostic and therapy imaging devices can be a burden on the financials of both the hospitals as well as the original equipment manufacturers (OEMs). In the current era of connectivity, it is easier to get these devices connected to a standard monitoring station. Once the system is connected, OEMs can monitor the health of these devices remotely and take corrective actions by providing preventive maintenance thereby avoiding major unplanned downtime...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29038866/complete-genome-sequence-of-a-novel-avian-paramyxovirus-isolated-from-wild-birds-in-south-korea
#14
Jipseol Jeong, Youngsik Kim, Injung An, Seung-Jun Wang, Yongkwan Kim, Hyun-Jeong Lee, Kang-Seuk Choi, Se-Pyeong Im, Wongi Min, Jae-Ku Oem, Weonhwa Jheong
A novel avian paramyxovirus (APMV), Cheonsu1510, was isolated from wild bird feces in South Korea and serologically and genetically characterized. In hemagglutination inhibition tests, antiserum against Cheonsu1510 showed low reactivity with other APMVs and vice versa. The complete genome of Cheonsu1510 comprised 15,408 nucleotides, contained six open reading frames (3'-N-P-M-F-HN-L-5'), and showed low sequence identity to other APMVs (< 63%) and a unique genomic composition. Phylogenetic analysis revealed that Cheonsu1510 was related to but distinct from APMV-1, -9, and -15...
January 2018: Archives of Virology
https://www.readbyqxmd.com/read/28956841/on-the-material-characterisation-of-wind-turbine-blade-coatings-the-effect-of-interphase-coating-laminate-adhesion-on-rain-erosion-performance
#15
Enrique Cortés, Fernando Sánchez, Anthony O'Carroll, Borja Madramany, Mark Hardiman, Trevor M Young
Rain erosion damage, caused by repeated droplet impact on wind turbine blades, is a major cause for concern, even more so at offshore locations with larger blades and higher tip speeds. Due to the negative economic influence of blade erosion, all wind turbine Original Equipment Manufacturers (OEMs) are actively seeking solutions. In most cases, since the surface coating plays a decisive role in the blade manufacture and overall performance, it has been identified as an area where a solution may be obtained...
September 28, 2017: Materials
https://www.readbyqxmd.com/read/28942570/replacement-of-tifton-hay-by-spineless-cactus-in-girolando-post-weaned-heifers%C3%A2-diets
#16
Leonardo José Assis de Barros, Marcelo de Andrade Ferreira, Julio César Vieira de Oliveira, Djalma Cordeiro Dos Santos, Juana Catarina Cariri Chagas, Adryanne Marjorie Souza Vitor Alves, Aghata Elins Moreira da Silva, Wandemberg Rocha Freitas
This study aims to evaluate the effect of the Tifton 85 hay (Cynodon dactylon L. Pers) replacement by Orelha de Elefante Mexicana spineless cactus (Opuntia stricta [Haw.] Haw) on the nutrient intake and digestibility, growth performance, microbial protein synthesis, and efficiency of dietary nitrogen compound utilization in the diets of Girolando heifers. Twenty-four (3.5 months and 100 kg ± 3.5 kg) Girolando heifers (5/8 Holstein x Gyr) were used and arranged in a completely randomized design. Dry matter, organic matter, crude protein, and neutral detergent fiber decreased linearly as a function of the levels of Tifton hay (TH) replacement by Orelha de Elefante Mexicana spineless cactus (OEM)...
January 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28929477/superstorm-sandy-emergency-management-staff-perceptions-of-impact-and-recommendations-for-future-preparedness-new-york-state
#17
Adam Yanson, Asante Shipp Hilts, Stephanie Mack, Millicent Eidson, Trang Nguyen, Guthrie Birkhead
OBJECTIVE: This study collected and summarized feedback from staff at the New York State (NYS) Office of Emergency Management (OEM) and three county OEMs within NYS to understand lessons learned from the 2012 Superstorm Sandy. DESIGN: Cross-sectional qualitative and quantitative analysis. SUBJECTS, PARTICIPANTS: One staff person from each identified critical role from the state and county OEMs who were still employed in the roles identified...
July 2017: Journal of Emergency Management: JEM
https://www.readbyqxmd.com/read/28900762/novel-reassortant-clade-2-3-4-4-avian-influenza-a-h5n8-virus-in-a-grey-heron-in-south-korea-in-2017
#18
Chanjin Woo, Jung-Hoon Kwon, Dong-Hun Lee, Youngsik Kim, Kwanghee Lee, Seong-Deok Jo, Ki Dong Son, Jae-Ku Oem, Seung-Jun Wang, Yongkwan Kim, Jeonghwa Shin, Chang-Seon Song, Weonhwa Jheong, Jipseol Jeong
We report the identification of a novel reassortant clade 2.3.4.4 H5N8 virus from a dead grey heron in Korea in 2017. Outbreaks of clade 2.3.4.4 H5 HPAIVs have been reported worldwide, and they have evolved into multiple genotypes among wild birds. Phylogenetic analysis indicated that this virus likely originated from Qinghai Lake and Western Siberia and further evolved through reassortment with Eurasian LPAI during the 2016 fall migration of wild birds. Enhanced surveillance and comparative genetic analysis will help to monitor the further evolution and dissemination of clade 2...
December 2017: Archives of Virology
https://www.readbyqxmd.com/read/28841178/biosynthesis-of-silver-nanoparticles-on-orthodontic-elastomeric-modules-evaluation-of-mechanical-and-antibacterial-properties
#19
Alma E Hernández-Gómora, Edith Lara-Carrillo, Julio B Robles-Navarro, Rogelio J Scougall-Vilchis, Susana Hernández-López, Carlo E Medina-Solís, Raúl A Morales-Luckie
In the present study, silver nanoparticles (AgNPs) were synthesized in situ on orthodontic elastomeric modules (OEM) using silver nitrate salts as metal-ion precursors and extract of the plant Hetheroteca inuloides (H. inuloides) as bioreductant via a simple and eco-friendly method. The synthesized AgNPs were characterized by UV-visible spectroscopy; scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and transmission electron microscopy (TEM). The surface plasmon resonance peak found at 472 nm confirmed the formation of AgNPs...
August 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28725945/genetic-characteristics-of-coronaviruses-from-korean-bats-in-2016
#20
Saemi Lee, Seong-Deok Jo, Kidong Son, Injung An, Jipseol Jeong, Seung-Jun Wang, Yongkwan Kim, Weonhwa Jheong, Jae-Ku Oem
Bats have increasingly been recognized as the natural reservoir of severe acute respiratory syndrome (SARS), coronavirus, and other coronaviruses found in mammals. However, little research has been conducted on bat coronaviruses in South Korea. In this study, bat samples (332 oral swabs, 245 fecal samples, 38 urine samples, and 57 bat carcasses) were collected at 33 natural bat habitat sites in South Korea. RT-PCR and sequencing were performed for specific coronavirus genes to identify the bat coronaviruses in different bat samples...
January 2018: Microbial Ecology
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