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https://www.readbyqxmd.com/read/30023927/design-and-synthesis-of-aza-oxa-heterocycle-based-conjugates-as-novel-anti-inflammatory-agents-targeting-cyclooxygenase-2
#1
Sukhmeet Kaur, Priya Kumari, Gurjit Singh, Rajbir Bhatti, Palwinder Singh
A library of hybrid molecules was procured by the combination of triazine-indole adduct with morpholine/piperidine/pyrrolidine and pyrazole/pyrimidine/oxindole moieties. Enzyme immunoassays on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) identified compound 6 having an IC50 value of 20 nM for COX-2 and 3000 nM for COX-1. The significant reduction in the formation of prostaglandin E2 in the lipopolysaccharide-treated (COX-2-activated) human whole blood, almost no change in the production of thromboxane B2 in the calcium ionophore-treated (COX-1-activated) sample of human whole blood, and the mechanistic studies on Swiss albino mice ensured that compound 6 is selective for COX-2...
May 31, 2018: ACS Omega
https://www.readbyqxmd.com/read/30022638/using-low-coverage-whole-genome-sequencing-technique-to-analyze-the-chromosomal-copy-number-alterations-in-the-exfoliative-cells-of-cervical-cancer
#2
Tong Ren, Jing Suo, Shikai Liu, Shu Wang, Shan Shu, Yang Xiang, Jing He Lang
OBJECTIVES: We analyzed the chromosomal-arm-level copy number alterations (CNAs) in the cervical exfoliative cell and tissue samples by using the low-coverage whole genomic sequencing technique. METHODS: In this study, we retrospectively collected 55 archived exfoliated cervical cell suspension samples and the corresponding formalin-fixed and paraffin-embedded tissue section samples including 27 invasive cervical cancer and 28 control cases. We also collected 19 samples of the cervical exfoliative cells randomly from women to verify the new algorithm model...
June 29, 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/30022610/ddpcr-applied-on-archived-continuous-plankton-recorder-samples-reveals-long-term-occurrence-of-class-1-integrons-and-a-sulphonamide-resistance-gene-in-marine-plankton-communities
#3
Andrea Di Cesare, Sara Petrin, Diego Fontaneto, Carmen Losasso, Ester M Eckert, Giovanni Tassistro, Alessio Borello, Antonia Ricci, William H Wilson, Carla Pruzzo, Luigi Vezzulli
Antibiotic resistance is a rising threat for human health. Although in clinical settings and terrestrial environments the rise of antibiotic resistant bacteria is well documented, their dissemination and spread in the marine environment, covering almost two-thirds of the Earth's surface, is still poorly understood. In this study, the presence and abundance of sulphonamide resistance gene (sul2) and class 1 integron-integrase gene (intI1), used as markers for the occurrence and spread of antibiotic resistance genes since the beginning of the antibiotic era, were investigated...
July 18, 2018: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/30021865/hotspot-daxx-ptch2-and-cyfip2-mutations-in-pancreatic-neuroendocrine-neoplasms
#4
Timon Vandamme, Matthias Beyens, Gitta Boons, Anne Schepers, Kimberly Kamp, Katharina Biermann, Patrick Pauwels, Wouter W de Herder, Leo Hofland, Marc Peeters, Guy Van Camp, Ken Op de Beeck
Mutations in DAXX/ATRX, MEN1, and genes involved in the phosphoinositide-3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway have been implicated in pancreatic neuroendocrine neoplasms (pNENs). However, mainly mutations present in the majority of tumor cells have been identified, while proliferation-driving mutations could be present only in small fractions of the tumor. This study aims to identify high- and low-abundance mutations in pNENs using ultra-deep targeted resequencing. Formalin-fixed paraffin-embedded matched tumor-normal tissue of 38 well-differentiated pNENs was sequenced using a HaloPlex targeted resequencing panel...
July 18, 2018: Endocrine-related Cancer
https://www.readbyqxmd.com/read/30021645/no-evidence-for-human-papillomavirus-having-a-causal-role-in-salivary-gland-tumors
#5
Linnea Haeggblom, Ramona Gabriela Ursu, Leila Mirzaie, Tove Attoff, Caroline Gahm, Lalle Hammarstedt Nordenvall, Anders Näsman
BACKGROUND: Salivary gland malignancies are a very heterogeneous group of cancers, with histologically > 20 different subtypes, and prognosis varies greatly. Their etiology is unknown, however, a few small studies show presence of human papillomavirus (HPV) in some subtypes, although the evidence for HPV having a causal role is weak. The aim of this study was to investigate if HPV plays a causal role in the development of different parotid salivary gland tumor subtypes. METHODS: DNA was extracted from 107 parotid salivary gland formalin fixed paraffin embedded tumors and 10 corresponding metastases, and tested for 27 different HPV types using a multiplex bead based assay...
July 18, 2018: Diagnostic Pathology
https://www.readbyqxmd.com/read/30021352/oncogenic-mir-663a-is-associated-with-cellular-function-and-poor-prognosis-in-renal-cell-carcinoma
#6
Liang Zhou, Xiang Pan, Zuwei Li, Peijie Chen, Jing Quan, Canbin Lin, Yulin Lai, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Yaoting Gui, Yongqin Lai
BACKGROUND: MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported. METHODS: In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2)...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30019318/clinical-validation-of-the-next-generation-sequencing-based-extended-ras-panel-assay-using-metastatic-colorectal-cancer-patient-samples-from-the-phase-3-prime-study
#7
Nitin Udar, Catherine Lofton-Day, Jun Dong, Darcy Vavrek, A Scott Jung, Kristen Meier, Anita Iyer, Ryan Slaughter, Karen Gutekunst, Bruce A Bach, Marc Peeters, Jean-Yves Douillard
PURPOSE: To validate a next-generation sequencing (NGS)-based companion diagnostic using the MiSeqDx® sequencing instrument to simultaneously detect 56 RAS mutations in DNA extracted from formalin-fixed paraffin-embedded metastatic colorectal cancer (mCRC) tumor samples from the PRIME study. The test's ability to identify patients with mCRC likely to benefit from panitumumab treatment was assessed. METHODS: Samples from PRIME, which compared first-line panitumumab + FOLFOX4 with FOLFOX4, were processed according to predefined criteria using a multiplex assay that included input DNA qualification, library preparation, sequencing, and the bioinformatics reporting pipeline...
July 17, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/30019121/nuclear-localization-of-robo-is-associated-with-better-survival-in-bladder-cancer
#8
Ulrich Krafft, Henning Reis, Marc Ingenwerth, Ilona Kovalszky, Markus Becker, Christian Niedworok, Christopher Darr, Péter Nyirády, Boris Hadaschik, Tibor Szarvas
The Slit-Robo pathway has shown to be altered in several malignant diseases. However, its role in bladder cancer is poorly understood. Therefore, we aimed to assess the tissue expression of Robo1 and Robo4 as well as their ligand Slit2 in different stages of bladder cancer to explore possible changes of Slit-Robo signalling during the progression of bladder cancer. Robo1, Robo4 and Slit2 gene expressions were analyzed in 92 frozen bladder cancer tissue samples by using reverse transcription quantitative real-time PCR...
July 17, 2018: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/30018734/the-immune-related-microrna-mir-146b-is-upregulated-in-glioblastoma-recurrence
#9
Shariq S Khwaja, Chunyu Cai, Shahed N Badiyan, Xiaowei Wang, Jiayi Huang
Background: Glioblastoma (GBM) has a high rate of local recurrence despite chemoradiotherapy (CRT). Genome-wide expression profiling was performed on patient tumors before and after chemoradiotherapy to identify genes and gene pathways associated with recurrence. Results: Median time to recurrence was 8.9 months with median time to second surgery of 9.6 months. The microRNA (miRNA) analysis identified 9 oncologic and immune-related miRNAs to be differentially expressed, including the hypoxia-related miR-210 and the immune-modulatory miR-146b...
June 26, 2018: Oncotarget
https://www.readbyqxmd.com/read/30018727/identification-of-mir-18a-5p-as-an-oncogene-and-prognostic-biomarker-in-rcc
#10
Liang Zhou, Zuwei Li, Xiang Pan, Yulin Lai, Jing Quan, Liwen Zhao, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Shangqi Yang, Yaoting Gui, Yongqing Lai
BACKGROUND: RCC is a malignant tumor that originates from renal tubular epithelial cells, accounting for nearly 90% of renal malignancies and 3% of adult malignancies. It was reported that more than 30-40% of patients with early localized RCC still have recurrence and metastasis after receiving radical surgery. miRNAs are an endogenous non-coding small RNAs that play an important role in the regulation of tumor cell proliferation, differentiation and apoptosis. METHODS: In our study, RT-qPCR, CCK-8 assay, wound scratch assay, transwell assay and flow cytometry assay were designed to identify the expression and functions of miR-18a-5p in RCC...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/30018493/effect-of-chlorine-dioxide-gas-application-to-egg-surface-microbial-reduction-effect-quality-of-eggs-and-hatchability
#11
Hansung Chung, Hyobi Kim, Donghoon Myeong, Seongjoon Kim, Nong-Hoon Choe
Controlling of microorganisms in the industrial process is important for production and distribution of hatching and table eggs. In the previous study, we reported that chlorine dioxide (ClO2 ) gas of a proper concentration and humidity can significantly reduce the load of Salmonella spp. on eggshells. In this study, we compared microbial reduction efficacy on egg's surface using hatching eggs and table eggs, internal quality of table eggs, and hatchability after both the conventional method (washing and UV expose, fumigation with formalin) and ClO2 gas disinfection...
July 2018: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/30018181/aging-exacerbates-mortality-of-acinetobacter-baumannii-pneumonia-and-reduces-the-efficacies-of-antibiotics-and-vaccine
#12
Hao Gu, Dong Liu, Xi Zeng, Liu-Sheng Peng, Yue Yuan, Zhi-Fu Chen, Quan-Ming Zou, Yun Shi
Pneumonia caused by Acinetobacter baumannii has become a serious threat to the elderly. However, there are no experimental studies on the relevance between aging and A. baumannii infections. Here, we established an aged pneumonia mouse model by non-invasive intratracheal inoculation with A. baumannii . Higher mortality was observed in aged mice along with increased bacterial burdens and more severe lung injury. Increased inflammatory cell infiltration and enhanced pro-inflammatory cytokines at 24 hours post infection were detected in aged mice than those in young mice...
July 18, 2018: Aging
https://www.readbyqxmd.com/read/30017147/immunization-regimen-in-asian-sea-bass-lates-calcarifer-broodfish-a-practical-strategy-to-control-vertical-transmission-of-nervous-necrosis-virus-during-seed-production
#13
Rolando Pakingking, Evelyn Grace de Jesus-Ayson, Ofelia Reyes, Norwell Brian Bautista
Outbreaks of viral nervous necrosis (VNN) in Asian sea bass (Lates calcarifer) at the larval stages via vertical transmission of nervous necrosis virus (NNV) from asymptomatic broodfish remain as a major deterrent during seed production. A five-year study was conducted to produce NNV-specific-free sea bass broodfish reared in land-based tanks through an annual immunization regimen with the formalin-inactivated NNV. We primarily immunized (intraperitoneal injection) sea bass juveniles (5 g) and monitored the neutralizing antibody (Nab) titers in the sera of these fish at scheduled intervals post-immunization...
July 14, 2018: Vaccine
https://www.readbyqxmd.com/read/30016858/prognostic-significance-of-ephb2-expression-in-colorectal-cancer-progression
#14
Bo Gun Jang, Hye Sung Kim, Weon Young Chang, Jung Mo Bae, Gyeong Hoon Kang
Background: A receptor tyrosine kinase for ephrin ligands, EPHB2, is expressed in normal colorectal tissues and colorectal cancers (CRCs). The aim of this study was to investigate EPHB2 expression over CRC progression and determine its prognostic significance in CRC. Materials and Methods: To measure EPHB2 mRNA and protein expression, real-time PCR and immunohistochemistry were performed in 32 fresh-frozen and 567 formalin-fixed paraffin-embedded CRC samples, respectively...
July 18, 2018: Journal of Pathology and Translational Medicine
https://www.readbyqxmd.com/read/30016232/pasteurella-multocida-inactivated-with-ferric-chloride-and-adjuvanted-with-bacterial-dna-is-a-potent-and-efficacious-vaccine-in-balb-c-mice
#15
Maryam Homayoon, Yahya Tahamtan, Mohammad Kargar, Seyed Mohammad Hossein Hosseini, Abbas Akhavan Sepahy
PURPOSE: Pasteurella multocida (P. multocida) is a principal pathogen of domestic animals and an opportunistic pathogen of humans. It is the causative agent of pneumonia and haemorrhagic septicaemia in cattle, sheep and goats, fowl cholera in chickens and progressive atrophic rhinitis in swine. In this study, we investigated the humoral and cellular immune responses and protective immunity conferred by an iron-inactivated vaccine with bacterial DNA (IIV+bDNA) as an adjuvant in mice. METHODOLOGY: P...
July 17, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30015848/aryl-hydrocarbon-receptor-a-tumor-grade%C3%A2-associated-marker-of-oral-cancer-is-directly-downregulated-by-polydatin-a-pilot-study
#16
Manuela Martano, Paola Stiuso, Angelo Facchiano, Salvatore De Maria, Daniela Vanacore, Brunella Restucci, Corrado Rubini, Michele Caraglia, Giampietro Ravagnan, Lorenzo Lo Muzio
Oral squamous cell carcinoma (OSCC) is one of the most aggressive and deadliest tumors worldwide. The aryl hydrocarbon receptor (AHR) is a nuclear transcription factor known as a dioxin receptor and mediates the toxic effects of industrial contaminants. In addition, AHR has been implicated in multiple cellular processes and its expression has been shown to play a critical role in tumorigenesis, including human oral cancer cell lines. In the present study, we evaluated the expression of AHR/HSP-90 in 25 formalin‑fixed, paraffin-embedded human oral cancer specimens by IHC analysis...
July 10, 2018: Oncology Reports
https://www.readbyqxmd.com/read/30015689/a-contemporary-review-about-the-management-of-radiation-induced-hemorrhagic-cystitis
#17
Antoine Dautruche, Guila Delouya
PURPOSE OF REVIEW: There are various specific therapeutic intervention available to treat hemorrhagic cystitis, once emergency treatment has been carried out. The lack of prospective studies, because of the relative rarity of this condition, makes it difficult to hierarchize the therapeutic sequence. The present review presents and summarizes the literature published on radiation-induced hemorrhagic cystitis from April 2015, date of a precedent exhaustive review, to March 2018. RECENT FINDINGS: During our period of interest, 13 clinical studies and two new clinical trials protocols were published...
July 14, 2018: Current Opinion in Supportive and Palliative Care
https://www.readbyqxmd.com/read/30015382/biallelic-tumor-suppressor-loss-and-dna-repair-defects-in-de-novo-small-cell-prostate-cancer
#18
Edmund Cp Chedgy, Gillian Vandekerkhove, Cameron Herberts, Matti Annala, Adam J Donoghue, Michael Sigouros, Elie Ritch, Werner Struss, Saki Konomura, Janet Liew, Sunil Parimi, Joanna Vergidis, Antonio Hurtado-Coll, Andrea Sboner, Ladan Fazli, Himisha Beltran, Kim N Chi, Alexander W Wyatt
Small cell prostatic carcinoma (SCPC) is an aggressive pathology that is managed similarly to small cell lung cancer. SCPC can evolve from prostatic adenocarcinoma in response to androgen deprivation therapy, but in rare cases is present at initial cancer diagnosis. The molecular etiology of de novo SCPC is incompletely understood, due to the scarcity of tumor tissue and the short life expectancy of patients. Through a retrospective search of our regional oncology pharmacy database, we identified 18 patients diagnosed with de novo SCPC between 2004 and 2017...
July 17, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/30014985/method-for-preclinical-pathology-evaluation-and-analysis-of-cardiovascular-implantable-electronic-device-implant-sites
#19
Staci L Jessen, Maureen T O'Brien, Trevor W Lancon, Harrison R Smith, Leslie L Suarez, Matthew D Wilcox, Matthew Miller, Brad R Weeks, Fred J Clubb
Cardiovascular implantable electronic devices (CIEDs) typically incorporate leads that directly contact the endocardium. Post-explant pathology evaluation of formalin-fixed CIED lead implant sites and downstream organs (i.e., lungs) can provide useful safety data to the US Food and Drug Administration; however, current regulatory guidelines do not mandate how the safety data are collected. In this paper, we outline a protocol for preclinical pathology evaluation of leads associated with CIEDs, which includes formalin fixation of the heart and lungs, gross evaluation, and qualitative and quantitative histologic evaluation...
May 18, 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/30013339/improved-sensitivity-of-cellular-mri-using-phase-cycled-balanced-ssfp-of-ferumoxytol-nanocomplex-labeled-macrophages-at-ultrahigh-field
#20
Yelong Shen, Lirong Yan, Xingfeng Shao, Bin Zhao, Jinlun Bai, Wange Lu, Danny Jj Wang
Purpose: The purpose of this study was to investigate the feasibility and sensitivity of cellular magnetic resonance imaging (MRI) with ferumoxytol nanocomplex-labeled macrophages at ultrahigh magnetic field of 7 T. Materials and methods: THP-1-induced macrophages were labeled using self-assembling heparin + protamine + ferumoxytol nanocomplexes which were injected into a gelatin phantom visible on both microscope and MRI. Susceptibility-weighted imaging (SWI) and balanced steady-state free precession (bSSFP) pulse sequences were applied at 3 and 7 T...
2018: International Journal of Nanomedicine
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