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https://www.readbyqxmd.com/read/30444580/immunogenicity-of-a-bivalent-protein-as-a-vaccine-against-edwardsiella-anguillarum-and-vibrio-vulnificus-in-japanese-eel-anguilla-japonica
#1
Songlin Guo, Linlin Hu, Jianjun Feng, Peng Lin, Le He, Qingpi Yan
The OMPs A (OmpA)-of Edwardsiella anguillarum and OmpU of V. vulnificus have been proven to be good antigens. In this study, after construction of a vector, a new recombinant Omp (rOMP) containing both OmpA and OmpU was expressed and purified. Then, the Japanese eels (Anguilla japonica) were intraperitoneally (i.p.) injected with the phosphate-buffered saline (PBS group), formalin-killed-cell (FKC group) or the recombinant Omp (rOMP group). The stimulation index of the whole blood cells in eels from FKC group was significantly higher than the eels from PBS and rOMP groups at 28 dpi; serum titers of anti-E...
November 16, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/30443013/keratin-17-is-a-sensitive-and-specific-biomarker-of-urothelial-neoplasia
#2
Sruthi Babu, Daniel C Mockler, Lucia Roa-Peña, Agnieszka Szygalowicz, Nam W Kim, Sholeh Jahanfard, Shahram S Gholami, Richard Moffitt, John P Fitzgerald, Luisa F Escobar-Hoyos, Kenneth R Shroyer
There is a clinical need to identify novel biomarkers to improve diagnostic accuracy for the detection of urothelial tumors. The current study aimed to evaluate keratin 17 (K17), an oncoprotein that drives cell cycle progression in cancers of multiple anatomic sites, as a diagnostic biomarker of urothelial neoplasia in bladder biopsies and in urine cytology specimens. We evaluated K17 expression by immunohistochemistry in formalin-fixed, paraffin embedded tissue specimens of non-papillary invasive urothelial carcinoma (UC) (classical histological cases), high grade papillary UC (PUC-LG), low grade papillary UC (PUC-HG), papillary urothelial neoplasia of low malignant potential (PUNLMP), and normal bladder mucosa...
November 15, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/30431717/expression-of-nedd9-in-transbronchial-biopsies-of-lung-adenocarcinoma
#3
Jelena Ostojić, Luka Brčić, Pero Hrabač, Sven Seiwerth
Lung cancer as the major cause of cancer mortality worldwide includes several histologic subtypes evolving from numerous genetic and epigenetic changes emerging in alveolar, bronchiolar and bronchial epithelium. The majority of cases are diagnosed in advanced stage (i.e. stages IIIB and IV), often with scanty amount of tissue in transbronchial biopsies or cytology specimens. The aim of the present study was to investigate the expression of the scaffolding protein neural precursor cell expressed, developmentally downregulated 9 (NEDD9) in small biopsies of lung adenocarcinoma...
June 2018: Acta Clinica Croatica
https://www.readbyqxmd.com/read/30431194/three-dimensional-nmr-microscopy-of-zebrafish-specimens
#4
Timothy L Kline, Caroline R Sussman, Maria V Irazabal, Prasanna K Mishra, Elisabeth A Pearson, Vicente E Torres, Slobodan I Macura
While zebrafish embryos in the first five days after fertilization are clear and amenable to optical analysis, older juveniles and adults are not, due to pigmentation development and tissue growth. Thus other imaging methods are needed to image adult specimens. NMR is a versatile tool for studies of biological systems and has been successfully used for in vivo zebrafish microscopy. In this work we use NMR microscopy (MRM) for assessment of zebrafish specimens, which includes imaging of formalin fixed (FF), formalin fixed and paraffin embedded (FFPE), fresh (unfixed), and FF gadolinium doped specimens...
November 15, 2018: NMR in Biomedicine
https://www.readbyqxmd.com/read/30430388/lc-esi-it-ms-ms-and-maldi-tof-approach-identification-of-natural-polymers-from-rhizophora-mangle-barks-and-determination-of-their-analgesic-and-anti-inflammatory-properties
#5
Leonardo Mendes de Souza Mesquita, Caroline Fabri Bittencourt Rodrigues, Cláudia Quintino da Rocha, Mayara Silveira Bianchim, Clenilson Martins Rodrigues, Vanda Maria de Oliveira, Henrique Hessel Gaeta, Mariana Novo Belchor, Marcos Hikari Toyama, Wagner Vilegas
We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3)...
November 14, 2018: Natural Products and Bioprospecting
https://www.readbyqxmd.com/read/30429960/b-973-a-novel-%C3%AE-7-nachr-ago-pam-racemic-and-asymmetric-synthesis-electrophysiological-studies-and-in-vivo-evaluation
#6
Sumanta Garai, Krishnamohan S Raja, Roger L Papke, Jeffrey R Deschamps, M Imad Damaj, Ganesh A Thakur
We report here the total synthesis of B-973 (five steps), a recently identified α7 nAChR ago-PAM, its enantiomeric resolution, and its electrophysiological characterization in Xenopus oocytes to identify (-)-B-973B as the bioactive enantiomer. The asymmetric synthesis of B-973B was accomplished in 99% ee, and X-ray crystallography studies revealed its absolute " S " stereochemistry. B-973B was effective in attenuating pain behavior and decreasing paw edema (formalin test), and its analgesic effects were mediated through α7 nAChR...
November 8, 2018: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/30428946/a-new-genus-and-three-new-species-of-anthocephaliidae-cestoda-rhinebothriidea-from-the-round-fantail-stingray-taeniurops-grabata-chondrichthyes-dasyatidae-from-the-mediterranean-sea-and-atlantic-ocean
#7
L Boudaya, L Neifar, L Euzet
The spiral intestines of a total of 16 round fantail stingrays Taeniurops grabata from the Mediterranean Sea off Tunisia were examined for cestodes. A new genus is erected in the Anthocephaliidae (Rhinebothridea) as Alveobothrium gen. n., with Alveobothrium grabatum sp. n. as its type species; the new genus differs from the other genera in the order in that its members possess bothridia with an apical sucker, marginal loculi and multiple staggered rows of facial loculi. Alveobothrium zarzisense sp. n. is also described...
November 15, 2018: Journal of Helminthology
https://www.readbyqxmd.com/read/30428380/preparation-of-porcine-enterotoxigenic-escherichia-coli-etec-ghosts-and-immunogenic-analysis-in-a-mouse-model
#8
Xuhua Ran, Xiaohong Chen, Shixia Wang, Chunlong Chang, Xiaobo Wen, Junjun Zhai, Hongbo Ni
Enterotoxignenic Escherichia coli (ETEC)-associated colibacillosis causes high levels of morbidity and mortality in neonatal piglets. Vaccination is among effective strategy to fight against ETEC-related diseases. Bacterial ghosts (BGs) are empty bacterial envelopes, which substain subtle antigenic comformation in bacterial outer membrane. In this study, a BG vaccine was generated using porcine ETEC isolated strain DQ061 and evaluated its safety and immunogenicity in a mouse model. The recombinant bacteria were constructed by transformation of lysis plasmid pHH43 and generation of BGs was conducted in a lysis rate of 99...
November 11, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/30427564/effects-of-glial-glutamate-transporter-activator-in-formalin-induced-pain-behavior-in-mice
#9
Ghallab Alotaibi, Shafiqur Rahman
BACKGROUND: Nociceptive pain remains a prevalent clinical problem and often poorly responsive to the currently available analgesics. Previous studies have shown that astroglial glutamate transporter-1 (GLT-1) in the hippocampus and anterior cingulate cortex (ACC) is critically involved in pain processing and modulation. However, the role of astroglial GLT-1 in nociceptive pain involving the hippocampus and ACC remains unknown. We investigated the role of 3-[[(2-Methylphenyl) methyl]thio]-6-(2-pyridinyl)-pyridazine (LDN-212320), a GLT-1 activator, in nociceptive pain model and hippocampal-dependent behavioral tasks in mice...
November 14, 2018: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/30426788/respiratory-syncytial-virus-vaccine-where-are-we-now-and-what-comes-next
#10
Asif Noor, Leonard R Krilov
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in infants and elderly and to date, there is no safe or effective vaccine against RSV. Areas Covered: This review provides a roadmap to RSV vaccine development. It is a journey spanning over more than half a century from the initial disappointment with inactivated formalin vaccine to the current advancements in vaccine technology. We highlight the important aspects of RSV structural biology and protective immune response...
November 14, 2018: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/30426328/comparison-of-three-real-time-pcr-assays-for-the-detection-of-pik3ca-somatic-mutations-in-formalin-fixed-paraffin-embedded-tissues-of-patients-with-breast-carcinomas
#11
A Lambert, J Salleron, M Lion, M Rouyer, N Lozano, A Leroux, J L Merlin, Alexandre Harlé
Breast cancer is the leading cause of cancer-related death in women worldwide. Mutations of the PIK3CA gene are found in approximately 25% of breast carcinomas and are reported as activators of the PI3K/AKT/mTOR pathway. This study aims to compare three assays for the somatic mutation detection of PIK3CA gene in FFPE tissues of patients with breast cancer. We compared Cobas® PIK3CA Mutation Test (Roche Diagnostics, Meylan, France), PCR amplification-refractory mutation system Scorpions® (ARMS) and High-Resolution Melting PCR assay (HRM) for the detection of PIK3CA mutations...
November 13, 2018: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/30425527/notch1-serves-as-a-prognostic-factor-and-regulates-metastasis-via-regulating-egfr-expression-in-hypopharyngeal-squamous-cell-carcinoma
#12
Jiajun Tian, Xianfang Liu, Xiuxiu Liu, Peihang Jing, Na Sa, Haibo Wang, Wei Xu
Objective: Hypopharyngeal squamous cell carcinoma (HSCC) remains one of the most lethal malignancies in head and neck. Notch1 has been validated to play prominent roles in the occurrence and development of various types of cancer. The aim of this study was to explore the function and underlying mechanism of Notch1 in HSCC. Patients and methods: Seventy-one cancer tissue samples and adjacent noncancerous formalin-fixed paraffin embedded tissue specimens were analyzed by immunohistochemistry...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/30425334/amphiregulin-in-intestinal-acute-graft-versus-host-disease-a-possible-diagnostic-and-prognostic-aid
#13
Khalid Amin, Usman Yaqoob, Brittney Schultz, Byron P Vaughn, Alexander Khoruts, Justin R Howard, Todd E DeFor, Colleen Forster, Carolyn Meyer, Isha Gandhi, Daniel J Weisdorf, Armin Rashidi, Margaret L MacMillan, Bruce R Blazar, Angela Panoskaltsis-Mortari, Shernan G Holtan
Amphiregulin, a weak epidermal growth factor receptor agonist, is elevated, while epidermal growth factor, a strong epidermal growth factor receptor agonist, is low in the blood of patients with severe acute graft-versus-host disease. However, the tissue expression and function of these epidermal growth factor receptor ligands in acute graft-versus-host disease target organs is unknown. We compared by immunohistochemistry expression of amphiregulin and epidermal growth factor in archived, formalin-fixed, paraffin-embedded intestinal tissues of 48 patients with biopsy-proven gastrointestinal acute graft-versus-host disease to 3 groups: (1) 10 non-hematopoietic cell transplant normal controls, (2) 11 patients with newly diagnosed ulcerative colitis (ulcerative colitis), (3) 8 patients with a clinical diagnosis of acute graft-versus-host disease despite pathologically non-diagnostic biopsies, (4) and 10 cases of cytomegalovirus colitis...
November 13, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/30425180/acgh-analysis-of-predictive-biomarkers-for-response-to-bevacizumab-plus-oxaliplatin-or-irinotecan-based-chemotherapy-in-patients-with-metastatic-colorectal-cancer
#14
Yoshihiko Fujita, Masataka Taguri, Kentaro Yamazaki, Junji Tsurutani, Kazuko Sakai, Takahiro Tsushima, Michitaka Nagase, Hiroshi Tamagawa, Shinya Ueda, Takao Tamura, Yasushi Tsuji, Kohei Murata, Koichi Taira, Tadamichi Denda, Toshikazu Moriwaki, Sadao Funai, Takako Eguchi Nakajima, Kei Muro, Akihito Tsuji, Motoki Yoshida, Koichi Suyama, Takuya Kurimoto, Naotoshi Sugimoto, Eishi Baba, Nobuhiko Seki, Mikio Sato, Takaya Shimura, Narikazu Boku, Ichinosuke Hyodo, Takeharu Yamanaka, Kazuto Nishio
BACKGROUND: The randomized phase III study (WJOG4407G) showed equivalent efficacy between FOLFOX and FOLFIRI in combination with bevacizumab as the first-line treatment for metastatic colorectal cancer (mCRC). We studied whole genome copy number profiles using array-based comparative genomic hybridization (aCGH) analysis of tumor tissue samples obtained in this study. The aim of this study was to identify gene copy number alterations that could aid in selecting either FOLFOX or FOLFIRI in combination with bevacizumab for patients with mCRC...
November 13, 2018: Oncologist
https://www.readbyqxmd.com/read/30423196/loss-of-atrx-daxx-expression-and-alternative-lengthening-of-telomeres-in-uterine-leiomyomas
#15
Terhi V Ahvenainen, Netta M Mäkinen, Pernilla von Nandelstadh, Maija E A Vahteristo, Annukka M Pasanen, Ralf C Bützow, Pia M Vahteristo
BACKGROUND: Uterine leiomyomas (ULs) are the most common gynecologic tumors and affect 3 of every 4 women by the age of 50 years. The majority of ULs are classified as conventional tumors, whereas 10% represent various histopathological subtypes with features that mimic malignancy. These subtypes include cellular and mitotically active ULs and ULs with bizarre nuclei. Uterine leiomyosarcoma (ULMS), the malignant counterpart of UL, is an aggressive cancer with poor overall survival. The early diagnosis and preoperative differentiation of ULMS from UL are often challenging because their symptoms and morphology resemble one another...
November 13, 2018: Cancer
https://www.readbyqxmd.com/read/30418619/sources-of-erroneous-sequences-and-artifact-chimeric-reads-in-next-generation-sequencing-of-genomic-dna-from-formalin-fixed-paraffin-embedded-samples
#16
Simon Haile, Richard D Corbett, Steve Bilobram, Morgan H Bye, Heather Kirk, Pawan Pandoh, Eva Trinh, Tina MacLeod, Helen McDonald, Miruna Bala, Diane Miller, Karen Novik, Robin J Coope, Richard A Moore, Yongjun Zhao, Andrew J Mungall, Yussanne Ma, Rob A Holt, Steven J Jones, Marco A Marra
Tissues used in pathology laboratories are typically stored in the form of formalin-fixed, paraffin-embedded (FFPE) samples. One important consideration in repurposing FFPE material for next generation sequencing (NGS) analysis is the sequencing artifacts that can arise from the significant damage to nucleic acids due to treatment with formalin, storage at room temperature and extraction. One such class of artifacts consists of chimeric reads that appear to be derived from non-contiguous portions of the genome...
November 12, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/30418598/evaluating-for-human-herpesvirus-6-in-the-liver-explants-of-children-with-liver-failure-of-unknown-etiology
#17
Christine H Yang, Malaya K Sahoo, Megan Fitzpatrick, Audrey H Lau, Benjamin A Pinsky, Olivia M Martinez
Background: Liver failure of unknown etiology (LFUE) has a transplant-free survival rate of less than 25%. Human herpesvirus 6 (HHV-6) may be associated with LFUE, but existing studies are limited by small sample size. Methods: We identified all children who underwent liver transplant for LFUE at a single quaternary children's hospital. 51/65 cases were able to be age-matched with controls (children who underwent liver transplant for metabolic liver disease). Quantitative polymerase chain reaction (PCR) for HHV-6 was performed on DNA from formalin-fixed paraffin-embedded liver explant tissue...
November 10, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/30416077/genomically-annotated-risk-model-for-advanced-renal-cell-carcinoma-a-retrospective-cohort-study
#18
Martin H Voss, Albert Reising, Yuan Cheng, Parul Patel, Mahtab Marker, Fengshen Kuo, Timothy A Chan, Toni K Choueiri, James J Hsieh, A Ari Hakimi, Robert J Motzer
BACKGROUND: The Memorial Sloan Kettering Cancer Center (MSKCC) risk model is an established prognostic tool for metastatic renal-cell carcinoma that integrates clinical and laboratory data, but is agnostic to tumour genomics. Several mutations, including BAP1 and PBRM1, have prognostic value in renal-cell carcinoma. Using two independent clinical trial datasets of patients with metastatic renal-cell carcinoma, we aimed to study whether the addition of the mutation status for several candidate prognostic genes to the MSKCC model could improve the model's prognostic performance...
November 8, 2018: Lancet Oncology
https://www.readbyqxmd.com/read/30415743/immunohistochemical-expression-of-the-nicotinic-acetylcholine-receptor-nachr-subunits-in-the-human-placenta-and-effects-of-cigarette-smoking-and-preeclampsia
#19
R Machaalani, E Ghazavi, T Hinton, A Makris, A Hennessy
INTRODUCTION: Cigarette smoking (CS) and preeclampsia (PE), regulate the expression of nicotinic acetylcholine receptor (nAChR) subunits in the placenta, yet no data exist at the histological level. METHODS: Using immunohistochemistry of formalin fixed and paraffin embedded placental tissue, this study quantified the expression of nine nAChR subunits (α2, α3, α4, α5, α7, α9, β1, β2, δ) and compared the expression amongst four groups of non-smoker non-PE (controls, n = 8), smokers (n = 8), PE (n = 8), and those who were smokers with PE (smoke + PE, n = 4)...
November 2018: Placenta
https://www.readbyqxmd.com/read/30412858/clinical-validation-of-newly-developed-multiplex-kit-using-luminex-xmap-technology-for-detecting-simultaneous-ras-and-braf-mutations-in-colorectal-cancer-results-of-the-rasket-b-study
#20
Hiroya Taniguchi, Wataru Okamoto, Kei Muro, Kiwamu Akagi, Hiroki Hara, Tomohiro Nishina, Takeshi Kajiwara, Tadamichi Denda, Shuichi Hironaka, Toshihiro Kudo, Taroh Satoh, Takeharu Yamanaka, Yukiko Abe, Yoshiyuki Fukushima, Takayuki Yoshino
Detection of RAS and BRAF mutations is essential to determine the optimal treatment strategy for metastatic colorectal cancer (CRC). We prospectively evaluated the MEBGEN RASKET-B KIT (RASKET-B), a novel multiplex kit, simultaneously detecting 48 types of RAS mutations and the BRAF V600E mutation using Luminex xMAP technology. The aim was to obtain market approval for RASKET-B as an in vitro diagnostic (IVD) option in Japan. Genomic DNA was extracted from 302 formalin-fixed paraffin-embedded tissues obtained from CRC patients...
November 6, 2018: Neoplasia: An International Journal for Oncology Research
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