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Patricia A Pesavento, Cristina W Cunha, Hong Li, Kenneth Jackson, Donal O'Toole
A constraint on understanding the pathogenesis of malignant catarrhal fever (MCF) is the limited number of tools to localize infected cells. The amount of detectable virus, visualized in the past either by immunohistochemistry or in situ hybridization (ISH), has been modest in fixed or frozen tissues. This complicates our understanding of the widespread lymphoid proliferation, epithelial necrosis/apoptosis, and arteritis-phlebitis that characterize MCF. In this work, we developed a probe-based in situ hybridization assay targeting 2 ovine herpesvirus 2 (OvHV-2) genes, as well as their respective transcripts, in formalin-fixed tissues...
September 16, 2018: Veterinary Pathology
Ming Guo, Abha Khanna, Jianping Wang, Michelle D Williams, Neda Kalhor, Yun Gong, Li Xu, Erich M Sturgis, Gregg Staerkel
CONTEXT: - Human papillomavirus (HPV) DNA in situ hybridization (ISH) assay and p16 immunohistochemistry (IHC) are used to determine high-risk HPV status in formalin-fixed, paraffin-embedded (FFPE) tissues in oropharyngeal squamous cell carcinoma (SCC). Although high sensitivity and specificity for HPV can be obtained by combined p16-IHC and HPV DNA-ISH, the occasional discrepancy between these assays has prompted evaluation of Cervista HPV assays in FFPE tissue from patients with oropharyngeal SCC...
September 17, 2018: Archives of Pathology & Laboratory Medicine
Shoudong Chai, Yang Yu, Haiming Li, Mingxin Gao, Li Bo, Chengxiong Gu
Vein graft remains the most broadly applied vascular material in coronary artery bypass surgery. However, the restenosis rate of the vein bridge following angioplasty is high. The present study investigated the effect of medical adhesive on vascular intimal hyperplasia, in addition to the signal transduction mechanism. A total of 36 New Zealand white rabbits were divided into three groups at random, including the normal group, the surgery group and the medical adhesive spray group. Following surgery for transplantation of the left external jugular vein to the ipsilateral common carotid artery for 4 weeks, the thickness and area of the intima and media of the vessel were measured on formalin‑fixed, paraffin wax‑embedded pathological sections using hematoxylin‑eosin staining, and alterations in the expression of proliferating cell nuclear antigen (PCNA), platelet endothelial cell adhesion molecule 1 (PECAM‑1), vascular cell adhesion protein 1 (VCAM‑1), extracellular signal‑regulated kinase (ERK)1/2, and endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical staining, reverse transcription‑quantitative polymerase chain reaction analysis and western blotting...
September 17, 2018: Molecular Medicine Reports
K J Stanger, H McGregor, M Marenda, J M Morton, Jwa Larsen
AIMS: To assess the efficacy of an autogenous vaccine against Yersinia pseudotuberculosis III in preventing clinical disease and deaths due to yersiniosis in young Merino sheep, and to determine the effect of vaccination on the prevalence of faecal shedding of pathogenic Yersinia spp., daily liveweight gain, and development of antibodies to Yersinia spp. following vaccination and natural exposure. METHODS: In six groups (three groups each from two farms) of young Merino sheep, 148-150 animals were systematically allocated to be vaccinated twice with an autogenous, formalin killed bacterin vaccine containing Y...
September 16, 2018: New Zealand Veterinary Journal
Phaik-Leng Cheah, Jing Li, Lai-Meng Looi, Kean-Hooi Teoh, Diana Bee-Lan Ong, Mark J Arends
Background: Except for a few studies with contradictory observations, information is lacking on the possibility of association between DNA mismatch repair (MMR) status and the presence of cancer stem cells in colorectal carcinoma (CRC), two important aspects in colorectal carcinogenesis. Methods: Eighty (40 right-sided and 40 left-sided) formalin-fixed, paraffin-embedded primary CRC were immunohistochemically studied for CD133, a putative CRC stem cell marker, and MMR proteins MLH1, MSH2, MSH6 and PMS2...
2018: PeerJ
Song Ee Park, Kyunghee Park, Eunjin Lee, Ji-Yeon Kim, Jin Seok Ahn, Young-Hyuck Im, Choonghoon Lee, Hun Jung, Soo Youn Cho, Woong-Yang Park, Razvan Cristescu, Yeon Hee Park
This study explored the clinical implications of tumor mutational burden (TMB) in a well-defined HER2-positive metastatic breast cancer (MBC) patient population who had been previously treated but had subsequent disease progression. Whole exome sequencing was performed on formalin-fixed paraffin-embedded tumor samples and matched normal tissue. Among the 46 patients, 13 (28.3%) were estrogen receptor-positive and nine (19.6%) were progesterone receptor-positive by immunohistochemistry analysis. Twenty patients (43...
2018: Oncoimmunology
Katsutoshi Miura, Kanna Yamashita
Scanning acoustic microscopy (SAM) can assess tissue stiffness by calculating the speed of sound (SOS) through tissues. SOS increases as tissue stiffness increases. Sensitivity to protease digestion depends on protein type, concentration, and modification. We analyzed the SOS images of formalin-fixed paraffin-embedded skin sections from elderly, young, diabetic, and nondiabetic subjects, as well as chronic and acute wounds. SAM provided high-resolution histology similar to LM and revealed characteristic SOS alteration following pepsin treatment...
2018: Pathobiology of Aging & Age related Diseases
Jessie Gills, Ravan Moret, Xin Zhang, John Nelson, Grace Maresh, Linh Hellmers, Daniel Canter, M'Liss Hudson, Shams Halat, Marc Matrana, Michael P Marino, Jakob Reiser, Maureen Shuh, Eric Laborde, Maria Latsis, Sunil Talwar, Stephen Bardot, Li Li
High-grade urothelial cell carcinoma of the bladder has a poor prognosis when lymph nodes are involved. Despite curative therapy for clinically-localized disease, over half of the muscle-invasive urothelial cell carcinoma patients will develop metastases and die within 5 years. There are currently no described xenograft models that consistently mimic urothelial cell carcinoma metastasis. To develop a patient-derived orthotopic xenograft model to mimic clinical urothelial cell carcinoma progression to metastatic disease, the urothelial cell carcinoma cell line UM-UC-3 and two urothelial cell carcinoma patient specimens were doubly tagged with Luciferase/RFP and were intra-vesically (IB) instilled into NOD/SCID mice with or without lymph node stromal cells (HK cells)...
August 24, 2018: Oncotarget
X Z Zheng, Y Zhan, J H Ma, H W Xu, W X Zhi, T B Chen, X Y Qin, P Wang, L H Zhang, Y C Gu, Y L Jin, L Zhu, B Q Wu
Objective: To investigate the frequency of KRAS mutation in mucinous epithelial lesions of the endometrium, and analyze the correlation between KRAS mutation and the clinicopathologic features. Methods: The cohort included forty-three cases of mucinous epithelial lesions of the endometrium selected from July 2015 to October 2017 from Beijing Obstetrics and Gynecology Hospital, and 22 control cases. Genomic DNA was extracted from formalin-fixed paraffin-embedded tissue sections. Polymerase chain reaction amplification for KRAS exons 2 and 3 was performed, followed by sequencing using capillary electrophoresis...
September 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
Juliana Machado-Rugolo, Alexandre Todorovic Fabro, Daniel Ascheri, Cecília Farhat, Alexandre Muxfeldt Ab'Saber, Vanessa Karen de Sá, Maria Aparecida Nagai, Teresa Takagaki, Ricardo Terra, Edwin Roger Parra, Vera Luiza Capelozzi
To demonstrate the usefulness of complementary next-generation sequencing (NGS) and immunohistochemistry (IHC) counting, we analyzed 196 patients with non-small cell lung cancer (NSCLC) who underwent surgical resection and adjuvant chemotherapy. Formalin-fixed paraffin-embedded samples of adenocarcinoma (ADC), squamous cell carcinoma (SqCC), and large-cell carcinoma (LCC) were used to prepare tissue microarrays and were examined by protein H-score IHC image analysis and NGS for oncogenes and proto-oncogenes and genes of tumor suppressors, immune checkpoints, epithelial-mesenchymal transition factors, tyrosine kinase receptors, and vascular endothelial growth factors (VEGFs)...
September 12, 2018: Human Pathology
Hellen Abreu, Fernanda Fogagnoli Simas, Fhernanda Ribeiro Smiderle, Vanessa Sovrani, Jorge Luiz Dallazen, Daniele Maria-Ferreira, Maria Fernanda Werner, Lucimara M C Cordeiro, Marcello Iacomini
A β-D-glucan was obtained from the edible mushroom Pholiota nameko by hot aqueous extraction and purification. NMR and methylation analyses of the purified fraction (GHW-PN, 1.46% yield) indicated the presence of a (1 → 3)-linked β-D-glucan, highly substituted (~27%) at O-6 by single units of β-D-Glcp or by (1 → 6)-β-D-Glcp fragments. The β-glucan (at 0.5, 1, and 2%) showed shear thinning behavior and when the concentration of the solution increased, there was an increase in apparent viscosity...
September 12, 2018: International Journal of Biological Macromolecules
Seiji Hosokawa, Kumiko Hosokawa, Gail Ishiyama, Akira Ishiyama, Ivan A Lopez
Megalin and cubilin are endocytic receptors expressed in many absorptive polarized epithelia. These receptors have been implicated in the transport of gentamicin in the inner ear as possible contributors to ototoxic damage. Megalin and cubilin have been characterized in detail in the mouse and rat inner ear, but not in the human inner ear. In this study, megalin and cubilin were localized by immunohistochemistry using affinity-purified antibodies in formalin fixed frozen cryostat and celloidin embedded sections of the human inner ear...
September 12, 2018: Brain Research
Claudia Teixeira da Costa Lodi, Márcia Antoniazi Michelin, Maria Inês Miranda Lima, Eddie Fernando Candido Murta, Letícia da Conceição Braga, Leticia Montes, Victor Hugo Melo
PURPOSE: Aberrant DNA methylation is present in virtually all types of human cancer. There is no clear evidence that methylation status can predict bad prognosis in patients with CIN recurrence in HIV infected. This study evaluates the relationship between aberrant methylation of CpG islands of CDH1, TIMP3 and MGMT genes and CIN recurrence in HIV-infected and -noninfected women. METHODS: This is a nested case-control study involving 33 cases with CIN recurrence and 114 controls without recurrence, HIV infected and noninfected, treated with LEEP, between 1999 and 2004...
September 14, 2018: Archives of Gynecology and Obstetrics
Hasan Mujtaba, Ying Wang, Yixin Duan, Meng Cao, Nana Zhang, Iffat Batool, Ali Murtaza, Xiaoli Chen, Yili Wang
BACKGROUND: The expected corresponding increase in tonsillar human papillomavirus (HPV) infection associated with increasing incidence of oropharyngeal squamous cell carcinoma (OPSCC) substantiate the evaluation of normal tonsillar tissue in different population. The epidemiology of HPV in tonsillar tissue varies geographically. This study evaluated samples from two countries to determine the prevalence in respective samples. OBJECTIVE: To characterize HPV infection in non-malignant tonsillar tissue from Shaanxi, China (herein after referred to as China) and Punjab, Pakistan (herein after referred to as Pakistan)...
September 6, 2018: Pathology, Research and Practice
Chuchard Punsawad, Nonthapan Phasuk, Suchirat Bunratsami, Kanjana Thongtup, Parnpen Viriyavejakul, Sarawoot Palipoch, Phanit Koomhin, Somchok Nongnaul
BACKGROUND: Soil-transmitted helminth (STH) infections are among the most common type of infections worldwide and are widely distributed in tropical areas. In rural areas of southern Thailand where most land is used for agriculture, children are at risk of acquiring parasites, especially STHs. Assessing the current situation regarding parasitic infection in these areas is a prerequisite for developing appropriate control measures. This study is aimed at determining the prevalence of intestinal parasitic infections, the intensity of STH infections and the associated risk factors among primary schoolchildren in Nopphitam District, Nakhon Si Thammarat Province, Thailand...
September 14, 2018: BMC Public Health
Daniel Kazdal, Rémi Longuespée, Steffen Dietz, Rita Casadonte, Kristina Schwamborn, Anna-Lena Volckmar, Jörg Kriegsmann, Katharina Kriegsmann, Margaux Fresnais, Albrecht Stenzinger, Holger Sültmann, Arne Warth, Mark Kriegsmann
PURPOSE: Matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI MSI) is a powerful tool to analyze the spatial distribution of peptides in tissues. Digital polymerase chain reaction (dPCR) is a method to reliably detect genetic mutations. Biopsy material is often limited due to minimally invasive techniques, but information on diagnosis, prognosis and prediction is required for subsequent clinical decision making. Thus, saving tissue material during diagnostic workup is highly warranted for best patient care...
September 14, 2018: Proteomics. Clinical Applications
Rémi Longuespée, Katharina Kriegsmann, Martin Cremer, Christiane Zgorzelski, Rita Casadonte, Daniel Kazdal, Jörg Kriegsmann, Wilko Weichert, Kristina Schwamborn, Margaux Fresnais, Peter Schirmacher, Mark Kriegsmann
BACKGROUND: In matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) standardized sample preparation is important to obtain reliable results. Herein, we investigated the impact of section thickness in formalin-fixed paraffin embedded (FFPE) tissue microarrays (TMA) on spectral intensities. PATIENTS AND METHODS: TMAs consisting of 10 different tissues represented by duplicates of 10 patients (n = 200 cores) were cut at 1, 3 and 5 μm...
September 14, 2018: Proteomics. Clinical Applications
Yujie Han, Xiaoying Li, Shasha Gao, Xianghong Liu, Lili Kang, Xiaomei Li, Yujie Lang, Xiao Li, Mingying Sun, Zhongtao Gai, Shenglin Yu
OBJECTIVE: Sepsis represents a complex disease with the dysregulated inflammatory response. The purpose of this study is to explore the role of interleukin 17 (IL-17, also known as IL-17A) in the occurrence and development of pediatric sepsis. METHODS: We established the sepsis neonatal rat model with the method of intraperitoneal injection of Escherichia coli (E coli). At each target time point, we got the blood from heart after anesthetizing animals, and the lung and liver tissues were fixed in formalin...
September 14, 2018: Journal of Cellular Biochemistry
Aline Stamford S G Gatis-Carrazzoni, Fernanda Virgínia Barreto Mota, Tonny Cley Campos Leite, Tatiane Bezerra de Oliveira, Sandra Cabral da Silva, Isla Vanessa Alves Bastos, Maria Bernadete de Souza Maia, Pedro Silvino Pereira, Pedro Paulo Marcelino Neto, Earl Celestino de Oliveira Chagas, Tania Maria Sarmento Silva, Márcia Silva do Nascimento, Teresinha Gonçalves da Silva
Some species of the genus Miconia are used in Brazilian folk medicine as analgesic and anti-inflammatory; however, several species of this genus are still poorly studied. Therefore, the aims of this study were to investigate the phytochemistry characterization by UPLC-DAD-QTOF-MS/MS, acute toxicity, anti-inflammatory and antinociceptive properties of Miconia minutiflora (Bonpl.) DC. The methanol extract of M. minutiflora (Mm-MeOH) was subjected to ultra-high-performance liquid chromatography (UPLC-DAD-QTOF-MS/MS) for the identification of the main phytocompounds...
September 13, 2018: Naunyn-Schmiedeberg's Archives of Pharmacology
Mayuko Ohuchi, Yasuo Sakamoto, Ryuma Tokunaga, Yuki Kiyozumi, Kenichi Nakamura, Daisuke Izumi, Keisuke Kosumi, Kazuto Harada, Junji Kurashige, Masaaki Iwatsuki, Yoshifumi Baba, Yuji Miyamoto, Naoya Yoshida, Takashi Shono, Hideaki Naoe, Yutaka Sasaki, Hideo Baba
The adenoma-carcinoma sequence, the sequential mutation and deletion of various genes by which colorectal cancer progresses, is a well-established and accepted concept of colorectal cancer carcinogenesis. Proteins of the polycomb repressive complex 2 (PRC2) function as transcriptional repressors by trimethylating histone H3 at lysine 27; the activity of this complex is essential for cell proliferation and differentiation. The histone methyltransferase enhancer of zeste homolog 2 (EZH2), an essential component of PRC2, is associated with the transcriptional repression of tumor suppressor genes...
October 2018: Oncology Letters
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