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Jiaxuan Guo, Shufang Wang, XiaoYang Yu, Rui Dong, Yuzhong Li, Xurong Mei, Yuanyue Shen
Polyamines (PAs) participate in many plant growth and developmental processes, including fruit ripening. However, it is not clear whether PAs play a role in the ripening of strawberry (Fragaria ananassa), a model non-climacteric plant. Here, we found that the content of the PA spermine (Spm) increased more sharply after the onset of fruit coloration than did that of the PAs putrescine (Put) or spermidine (Spd). Spm dominance in ripe fruit resulted from abundant transcripts of a strawberry S-adenosyl-L-methionine decarboxylase gene (FaSAMDC), which encodes an enzyme that generates a residue needed for PA biosynthesis...
March 9, 2018: Plant Physiology
Ryan C Fortenberry, Carlie M Novak, Joshua P Layfield, Eduard Matito, Timothy J Lee
Truncated, correlated, wave function methods either produce imaginary frequencies (in the extreme case) or non-physically low frequencies in out-of-plane motions for carbon and adjacent atoms when the carbon atoms engage in π bonding. Cyclopropenylidene is viewed as the simplest aromatic hydrocarbon, and the present as well as previous theoretical studies have shown that this simple molecule exhibits this behavior in the two out-of-plane bends (OPBs). This non-physical behavior has been treated by removing nearly linear dependent basis functions according to eigenvalues of the overlap matrix, by employing basis sets where the spd space saturation is balanced with higher angular momentum functions, by including basis set superposition/incompleteness error (BSSE/BSIE) corrections, or by combining standard correlation methods with explicitly-correlated methods to produce hybrid potential surfaces...
March 9, 2018: Journal of Chemical Theory and Computation
Hang Su, Yun-Tong Jiao, Fang-Fang Wang, Yue-E Liu, Wei-Li Niu, Guo-Tian Liu, Yan Xu
Putrescine and spermidine increase the transformation efficiency of Vitis vinifera L. cv. Thompson seedless. Accumulation of VpPR10.1 in transgenic V. vinifera Thompson seedless, likely increases its resistance to downy mildew. A more efficient method is described for facilitating Agrobacterium-mediated transformation of Vitis vinifera L. cv. Thompson Seedless somatic embryogenesis using polyamines (PAs). The efficacies of putrescine, spermidine and spermine are identified at a range of concentrations (10 µM, 100 µM and 1 mM) added to the culture medium during somatic embryo growth...
March 6, 2018: Plant Cell Reports
Wei Wei, Bin Xu, Yan Wang, Chen Wu, Jingting Jiang, Changping Wu
BACKGROUND: The prognostic significance of circulating soluble programmed death ligand-1 (sPD-L1) in patients with solid tumors remains unclear. We performed a meta-analysis to address this issue. METHODS: Several electronic databases were searched from January 1970 to May 2017. The hazard ratios (HRs) and 95% confidence interval (95% CI) were calculated to determine the relationship between the level of soluble PD-L1 in peripheral blood and patient overall survival...
January 2018: Medicine (Baltimore)
Santhosh Kalash Rajendrakumar, Kondareddy Cherukula, Hyeong Ju Park, Saji Uthaman, Yong Yeon Jeong, Byeong-Il Lee, In-Kyu Park
Stimuli-responsive polymeric nanoparticles are useful for overcoming challenges such as transfection efficiency and the specific and safe delivery of genes to cancer cells. Transfection outcomes can be improved through spatially and temporally controlled gene release. We formulated a nanoassembly comprising a disulfide-crosslinked polyethylenimine (ssPEI) conjugated with a tumor-specific cell-penetrating peptide (DS 4-3) (SPD) polyplex and bovine serum albumin (BSA)-loaded IR780 (BI) nanoparticle, thereby forming a dual-stimulus-triggered, tumor-penetrating and gene-carrying nanoassembly (BI-SPD) via electrostatic complexing...
February 27, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Ai Kanemura, Yuko Yoshikawa, Wakao Fukuda, Kanta Tsumoto, Takahiro Kenmotsu, Kenichi Yoshikawa
BACKGROUND: Polyamines have various biological functions including marked effects on the structure and function of genomic DNA molecules. Changes in the higher-order structure of DNA caused by polyamines are expected to be closely related to genetic activity. To clarify this issue, we examined the relationship between gene expression and the higher-order structure of DNA under different polyamine concentrations. PRINCIPAL FINDINGS: We studied the effects of polyamines, spermidine SPD(3+) and spermine SP(4+), on gene expression by a luciferase assay...
2018: PloS One
Yanxia Guo, Alice M Walsh, Mary Canavan, Mihir D Wechalekar, Suzanne Cole, Xuefeng Yin, Brittney Scott, Mathew Loza, Carl Orr, Trudy McGarry, Michele Bombardieri, Frances Humby, Susanna M Proudman, Costantino Pitzalis, Malcolm D Smith, Joshua R Friedman, Ian Anderson, Loui Madakamutil, Douglas J Veale, Ursula Fearon, Sunil Nagpal
Immune checkpoint blockade with therapeutic anti-cytotoxic T lymphocyte-associated antigen (CTLA)-4 (Ipilimumab) and anti-programmed death (PD)-1 (Nivolumab and Pembrolizumab) antibodies alone or in combination has shown remarkable efficacy in multiple cancer types, concomitant with immune-related adverse events, including arthralgia and inflammatory arthritis (IA) in some patients. Herein, using Nivolumab (anti-PD-1 antagonist)-responsive genes along with transcriptomics of synovial tissue from multiple stages of rheumatoid arthritis (RA) disease progression, we have interrogated the activity status of PD-1 pathway during RA development...
2018: PloS One
Andrew K Fenton, Sylvie Manuse, Josué Flores-Kim, Pierre Simon Garcia, Chryslène Mercy, Christophe Grangeasse, Thomas G Bernhardt, David Z Rudner
Most bacterial cells are surrounded by an essential cell wall composed of the net-like heteropolymer peptidoglycan (PG). Growth and division of bacteria are intimately linked to the expansion of the PG meshwork and the construction of a cell wall septum that separates the nascent daughter cells. Class A penicillin-binding proteins (aPBPs) are a major family of PG synthases that build the wall matrix. Given their central role in cell wall assembly and importance as drug targets, surprisingly little is known about how the activity of aPBPs is controlled to properly coordinate cell growth and division...
February 27, 2018: Proceedings of the National Academy of Sciences of the United States of America
Lihua Yang, Yang Shi, Jinjin Li, Ling Fang, Tiangang Luan
The potential of horseradish peroxidase (HRP) to catalyze the removal of sulfonamides from water and the effects of different H2 O2 and HRP concentrations were investigated. Six sulfonamides, each with a five- or six-membered heterocyclic group, including sulfamethoxazole (SMX), sulfathiazole (STZ), sulfapyridine (SPD), sulfadiazine (SDZ), sulfamerazine (SMR) and sulfamethoxypyridazine (SMP) were selected as target compounds. All sulfonamides exhibit a pseudo-first-order dependence of the concentration versus the reaction time...
February 20, 2018: Chemosphere
Miao Chen, Jingjing Chen, Na Luo, Rongda Qu, Zhenfei Guo, Shaoyun Lu
Dwarfism and drought tolerance are two valuable traits in breeding of many crops. In this study we report the novel physiological roles of cholesterol in regulation of plant growth and drought tolerance. Compared to the wild type, sterol-C24-methyltransferase 1 (SMT1) gene transcript was greatly reduced in a bermudagrass mutant with dwarfism and enhanced drought tolerance, accompanied with cholesterol accumulation, elevated transcript levels of a small group of genes including SAMDC, and increased concentrations of putrescine (Put), spermidine (Spd) and spermine (Spm)...
February 21, 2018: Plant, Cell & Environment
Shelley Mulligan, Sarah Schoen, Lucy Miller, Andrea Valdez, Aryanna Wiggins, Brianna Hartford, Amy Rixon
AIMS: This study examined the validity of a new measure of sensory processing for children, the Sensory Processing 3-Dimensions Scale (SP-3D). The SP-3D is a performance-based measure for children ages three to thirteen years, designed to assess sensory processing abilities, and identify the three patterns of sensory processing disorder (SPD) and related subtypes, including sensory modulation, sensory discrimination, and sensory-based motor disorders. METHODS: Age trends were explored using descriptive statistics and graphing techniques with a sample of children with and without SPD...
February 21, 2018: Physical & Occupational Therapy in Pediatrics
Wanquan Liu, Meifang Tan, Chunyan Zhang, Zhuofei Xu, Lu Li, Rui Zhou
Spermidine (Spd), spermine (Spm), and putrescine (Put), which are the most widely distributed cellular polyamines, are essential for normal growth and multiplication of both eukaryotic and prokaryotic cells. In this study, we identified the only putative polyamine transport system PotABCD in Streptococcus suis, a worldwide zoonotic Gram-positive pathogen causing lethal infections in humans and pigs. It was discovered that S. suis could uptake polyamines preferably Spd and Spm. By constructing a potA deleted mutant, we confirmed that PotABCD was responsible for polyamine uptake, and PotD bound to the protein of polyamines...
March 2018: Microbiological Research
Andrea C Klaver, Mary P Coffey, Jan O Aasly, David A Loeffler
Lysosome-associated membrane glycoprotein 2 (lamp2) plays critical roles in chaperone-mediated autophagy (CMA) and macroautophagy. Its isoform lamp2a is decreased in Parkinson's disease (PD) substantia nigra. Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are the most known common cause of late-onset PD; although LRRK2 is thought to regulate macroautophagy, the influence of LRRK2 mutations on lamp2 concentrations in the CNS is unknown. To examine this issue we compared lamp2 levels in cerebrospinal fluid (CSF) between sporadic PD (sPD) patients (n = 31), LRRK2 PD patients (n = 20), and healthy control subjects with or without LRRK2 mutations (LRRK2 CTL = 30, CTL = 27)...
March 15, 2018: Brain Research
Kun Cao, Jing Zhang, Xin-Yu Miao, Qiu-Xia Wei, Xin-Lu Zhao, Qing-Yu He, Xuesong Sun
Iron is an essential element for almost all bacteria. The iron ATP-binding cassette (ABC) transporters located on the cell membrane affects bacterial virulence and infection. Although a variety of Fe3+ -transporters have been found in bacteria, their evolutionary processes are rarely studied. Pneumococcal iron ABC transporter (PitA), a highly conserved Fe3+ -transporter in most pathogenic bacteria, influences the capsule formation and virulence of bacteria. However, multiple sequence alignment revealed that PitA is expressed in four different variants in bacteria, and the structural complexity of these variants increases progressively...
February 10, 2018: Journal of Inorganic Biochemistry
Chi C Chan, Philip R Szeszko, Edmund Wong, Cheuk Y Tang, Caitlin Kelliher, Justin D Penner, M Mercedes Perez-Rodriguez, Daniel R Rosell, Margaret McClure, Panos Roussos, Antonia S New, Larry J Siever, Erin A Hazlett
Abnormalities in temporal and frontal cortical volume, white matter tract integrity, and hemispheric asymmetry have been implicated in schizophrenia-spectrum disorders. Schizotypal personality disorder can provide insight into vulnerability and protective factors in these disorders without the confounds associated with chronic psychosis. However, multimodal imaging and asymmetry studies in SPD are sparse. Thirty-seven individuals with SPD and 29 healthy controls (HC) received clinical interviews and 3T magnetic resonance T1-weighted and diffusion tensor imaging scans...
February 14, 2018: Schizophrenia Research
He Chen, Qinglin Peng, Hanbo Yang, Liguo Yin, Jingli Shi, Yamei Zhang, Guochun Wang
OBJECTIVE: To investigate the levels of soluble programmed death ligand 1 (sPD-L1) and evaluate its association with malignancy in patients with dermatomyositis (DM). METHODS: Levels of sPD-L1 were measured in serum from 88 DM patients without malignancies (sDM), 40 with cancer-related DM (CRDM), and 30 healthy controls (HC) using ELISA. The CRDM subjects were divided into new-onset cancers (nCRDM) and stable cancers (sCRDM). Receiver-operating characteristic (ROC) curve analysis was performed to determine the cutoff sPD-L1 value that distinguished patients with nCRDM from those who were sDM...
February 1, 2018: Journal of Rheumatology
Sarah Sze Wah Wong, Manjusha Rani, Eswari Dodagatta-Marri, Oumaima Ibrahim-Granet, Uday Kishore, Jagadeesh Bayry, Jean-Paul Latgé, Arvind Sahu, Taruna Madan, Vishukumar Aimanianda
Surfactant protein D (SP-D), a C-type lectin and pattern-recognition soluble factor, plays an important role in immune surveillance to detect and eliminate human pulmonary pathogens. SPD has been shown to protect against infections with the most ubiquitous airborne fungal pathogen, <em>Aspergillus fumigatus</em>, but the fungal surface component(s) interacting with SP-D is unknown. Here, we show that SP-D binds to melanin pigment on the surface of <em>A. fumigatus</em> dormant spores (conidia)...
February 5, 2018: Journal of Biological Chemistry
Yuefei Ji, Yan Yang, Lei Zhou, Lu Wang, Junhe Lu, Corinne Ferronato, Jean-Marc Chovelon
The widespread occurrence of pharmaceuticals and their metabolites in natural waters has raised great concerns about their potential risks on human health and ecological systems. This study systematically investigates the degradation of sulfasalazine (SSZ) and its two human metabolites, sulfapyridine (SPD) and 5-aminosalicylic acid (5-ASA), by UV and UV/peroxydisulfate (UV/PDS) processes. Experimental results show that SPD and 5-ASA were readily degraded upon UV 254 nm direct photolysis, with quantum yields measured to be (8...
January 31, 2018: Water Research
Nazan Ulgen Tekerek, Basak Nur Akyildiz, Baris Derya Ercal, Sabahattin Muhtaroglu
OBJECTIVE: To detect the most effective biomarker to confirm ventilator associated pneumonia (VAP). METHODS: Fifty patients with VAP suspicious diagnosis and 30 healthy patients were recruited. Suspicion of VAP was established if patients met the modified CPIS score ≥ 6 points. The confirmation of VAP was defined by the quantitative culture of nonbronchoscopic bronchoalveolar lavage (BAL) >105 CFU/ml of pathogenic microorganism. Serum samples for determination of C-reactive protein (CRP), procalcitonin (PCT), pentraxin 3 (PTX3), surfactant protein D (SPD) were collected on suspected VAP...
February 2, 2018: Indian Journal of Pediatrics
Anne Laporte, Stéphanie Vandentorren, Marc-Antoine Détrez, Caroline Douay, Yann Le Strat, Erwan Le Méner, Pierre Chauvin
The Samenta study was conducted in 2009 in the Greater Paris area to estimate the prevalence of psychiatric disorders in homeless people. A cross-sectional survey was performed with a three-stage random sample of homeless people (n = 859), including users of day services, emergency shelters, hot meal distribution, long-term rehabilitation centres, and social hotels. Information was collected by a lay interviewer, using the Mini International Neuropsychiatric Interview, and completed by a psychologist through an open clinical interview...
January 31, 2018: International Journal of Environmental Research and Public Health
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