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https://www.readbyqxmd.com/read/28429371/design-of-a-short-non-uniform-acquisition-protocol-for-quantitative-analysis-in-dynamic-cardiac-spect-imaging-a-retrospective-123-i-mibg-animal-study
#1
Yunlong Zan, Yong Long, Kewei Chen, Biao Li, Qiu Huang, Grant T Gullberg
PURPOSE: Our previous works have found that quantitative analysis of (123) I-MIBG kinetics in the heart with dynamic single-photon emission computed tomography (SPECT) offers the potential to quantify the innervation integrity at an early stage of left ventricular hypertrophy. However, conventional protocols involving a long acquisition time for dynamic imaging reduce the animal survival rate and thus make longitudinal analysis difficult. The goal of this work was to develop a procedure to reduce the total acquisition time by selecting non-uniform acquisition times for projection views while maintaining the accuracy and precision of estimated physiologic parameters...
April 21, 2017: Medical Physics
https://www.readbyqxmd.com/read/28427536/health-risks-of-food-oxidation
#2
Mario Estévez, Zhuqing Li, Olugbenga P Soladoye, Thomas Van-Hecke
The impact of dietary habits on our health is indisputable. Consumer's concern on aging and age-related diseases challenges scientists to underline the potential role of food on the extension and guarantee of lifespan and healthspan. While some dietary components and habits are generally regarded as beneficial for our health, some others are being found to exert potential toxic effects and hence, contribute to the onset of particular health disorders. Among the latter, lipid and protein oxidation products formed during food production, storage, processing, and culinary preparation have been recently identified as potentially harmful to humans...
2017: Advances in Food and Nutrition Research
https://www.readbyqxmd.com/read/28415037/enantiomer-specific-accumulation-and-depuration-of-%C3%AE-hexabromocyclododecane-%C3%AE-hbcdd-in-chicken-gallus-gallus-as-a-tool-to-identify-contamination-sources
#3
Elsa Omer, Ronan Cariou, Adeline Huneau-Salaün, Gaud Dervilly-Pinel, Élisabeth Baéza, Angélique Travel, Sophie Le Bouquin, Catherine Jondreville, Bruno Le Bizec
A LC-ESI(-)-HRMS method dedicated to the analysis of 6 HBCDD enantiomers at trace levels in animal matrices was developed, using a cellulose based stationary phase with a particle size of 2.5 μm. This method was applied to a sample set derived from a kinetic study of α-HBCDD previously conducted in fast- and slow-growing chickens (Gallus gallus domesticus, n = 49, plus controls), in order to study the enantiomer specific accumulation and depuration of α-HBCDD in various tissues. Regarding abdominal adipose tissue, muscle and liver, the average enantiomeric fractions of α-HBCDD (EFα) for continuously exposed groups ranged between 0...
April 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28412936/-1-h-nmr-analysis-of-feces-new-possibilities-in-the-helminthes-infections-research
#4
Sarantos Kostidis, Daria Kokova, Natalia Dementeva, Irina V Saltykova, Hye Kyong Kim, Young Hae Choi, Oleg A Mayboroda
BACKGROUND: Analysis of the stool samples is an essential part of routine diagnostics of the helminthes infections. However, the standard methods such Kato and Kato-Katz utilize only a fraction of the information available. Here we present a method based on the nuclear magnetic resonance spectroscopy (NMR) which could be auxiliary to the standard procedures by evaluating the complex metabolic profiles (or phenotypes) of the samples. METHOD: The samples were collected over the period of June-July 2015, frozen at -20 °C at the site of collection and transferred within four hours for the permanent storage at -80 °C...
April 17, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28403128/an-oxygenated-and-transportable-machine-perfusion-system-fully-rescues-liver-grafts-exposed-to-lethal-ischemic-damage-in-a-pig-model-of-dcd-liver-transplantation
#5
Philippe Compagnon, Eric Levesque, Hassen Hentati, Mara Disabato, Julien Calderaro, Cyrille Feray, Anne Corlu, José Laurent Cohen, Ismail Ben Mosbah, Daniel Azoulay
BACKGROUND: Control of warm ischemia (WI) lesions that occur with donation after circulatory death (DCD) would significantly increase the donor pool for liver transplantation. We aimed to determine whether a novel, oxygenated and hypothermic machine perfusion device (HMP Airdrive system) improves the quality of livers derived from DCDs using a large animal model. METHODS: Cardiac arrest was induced in female large white pigs by IV injection of potassium chloride...
April 11, 2017: Transplantation
https://www.readbyqxmd.com/read/28399947/survival-of-macrobrachium-amazonicum-embryos-submitted-to-cooling
#6
Arthur Vinícius Lourenço Ferreira, Moisés Fernandes Martins, Míriam Luzia Nogueira Martins de Sousa, Aldeney Andrade Soares Filho, Célia Maria de Souza Sampaio
Cooling techniques have several applications for reproduction in aquaculture. However, few studies have sought to create protocols for cooling and cryopreservation of Macrobrachium amazonicum embryos. Thus, the objective of this work was to verify the survival of M. amazonicum embryos and the correlation between embryonic volume and mortality of M. amazonicum embryos after cooling. Embryo pools were collected from three females and divided into two treatment groups: dimethyl sulfoxide (DMSO) 3% and ethylene glycol (EG) 0...
April 12, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28379030/the-role-of-iron-in-the-pathogenesis-of-atherosclerosis
#7
P Kraml
Ferritin and increased iron stores first appeared on the list of cardiovascular risk factors more than 30 years ago and their causal role in the pathogenesis of atherosclerosis has been heavily discussed since the early 1990s. It seems that besides traditional factors such as hyperlipoproteinemia, hypertension, diabetes mellitus, obesity, physical inactivity, smoking and family history, high iron stores represent an additional parameter that could modify individual cardiovascular risk. The role of iron in the pathogenesis of atherosclerosis was originally primarily associated with its ability to catalyze the formation of highly reactive free oxygen radicals and the oxidation of atherogenic lipoproteins...
April 5, 2017: Physiological Research
https://www.readbyqxmd.com/read/28369966/the-effect-of-prefreeze-rejuvenation-on-postthaw-storage-of-red-blood-cells-in-as-3-and-sagm
#8
Charles C M Lelkens, Johan W M Lagerberg, Dirk de Korte
BACKGROUND: We investigated whether improving the metabolic status of red blood cell concentrates before freezing could extend the postthaw shelf life beyond 14 days while still meeting the requirements for hemolysis (0.8%) and total adenylate (>82% of original values). STUDY DESIGN AND METHODS: At Day 8 after collection, four leukoreduced red blood cell concentrates in saline-adenine-glucose-mannitol (SAGM) were pooled, mixed, and split (n = 4). Of these concentrates, two were rejuvenated in Rejuvesol...
March 28, 2017: Transfusion
https://www.readbyqxmd.com/read/28367770/effects-of-training-distance-on-feed-intake-growth-body-condition-and-muscle-glycogen-content-in-young-standardbred-horses-fed-a-forage-only-diet
#9
S Ringmark, T Revold, A Jansson
This study examined feed intake, growth, body condition, muscle glycogen content and nutrition-related health in 16 Standardbred horses fed a high-energy, forage-only diet ad libitum and allocated to either a control training programme (C-group) or a training programme with the high-intensity training distance reduced by 30% (R-group), from January as 2-year olds until December as 3-year olds. Feed intake was recorded on 10 occasions during 3 consecutive days. Body weight was recorded once in a week and height, body condition score (BCS), rump fat thickness and thickness of the m...
April 3, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28366869/long-term-nitrogen-and-phosphorus-enrichment-alters-vegetation-species-composition-and-reduces-carbon-storage-in-upland-soil
#10
William A V Stiles, Edwin C Rowe, Peter Dennis
Reactive nitrogen (N) deposition can affect ecosystem processes, particularly in oligotrophic upland habitats. Phosphorus (P) addition has been proposed to reduce the effects of N enrichment on N leaching and acidification, since P limitation can reduce biomass production and consequent sequestration of reactive N. However, biodiversity is often reduced in more productive ecosystems and P limitation may protect against this effect. Responses to P availability in instances of high N deposition are poorly understood...
March 30, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28366611/oscillatory-hydraulic-testing-as-a-strategy-for-napl-source-zone-monitoring-laboratory-experiments
#11
YaoQuan Zhou, Michael Cardiff
Non-aqueous phase liquids (NAPLs) have a complex mode of transport in heterogeneous aquifers, which can result in pools and lenses of NAPLs (the "source zone") that are difficult to detect and can cause long-term contamination via slow dissolution into groundwater (the "dissolved plume"). Characterizing the extent and evolution of NAPL contamination within the source zone is a useful strategy for designing and adapting appropriate remedial actions at many contaminated sites. As a NAPL flows into a given aquifer volume, the effective hydraulic conductivity (K) and specific storage (Ss) of the volume changes associated with the viscosity and compressibility of the impinging fluid, meaning that NAPL movement may be detectable with hydraulic testing...
March 21, 2017: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/28351817/do-forests-best-mitigate-co2-emissions-to-the-atmosphere-by-setting-them-aside-for-maximization-of-carbon-storage-or-by-management-for-fossil-fuel-substitution
#12
Anders Taeroe, Walid Fayez Mustapha, Inge Stupak, Karsten Raulund-Rasmussen
Forests' potential to mitigate carbon emissions to the atmosphere is heavily debated and a key question is if forests left unmanaged to store carbon in biomass and soil provide larger carbon emission reductions than forests kept under forest management for production of wood that can substitute fossil fuels and fossil fuel intensive materials. We defined a modelling framework for calculation of the carbon pools and fluxes along the forest energy and wood product supply chains over 200 years for three forest management alternatives (FMA): 1) a traditionally managed European beech forest, as a business-as-usual case, 2) an energy poplar plantation, and 3) a set-aside forest left unmanaged for long-term storage of carbon...
March 25, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28350667/acellular-hypothermic-extracorporeal-perfusion-extends-allowable-ischemia-time-in-a-porcine-whole-limb-replantation-model
#13
Maximilian Kueckelhaus, Alexander Dermietzel, Muayyad Alhefzi, Mario A Aycart, Sebastian Fischer, Nicco Krezdorn, Luccie Wo, Omar H Maarouf, Leonardo V Riella, Reza Abdi, Ericka M Bueno, Bohdan Pomahac
BACKGROUND: One of the major challenges in traumatic amputation is the need to keep ischemia time brief (4 to 6 hours) to avoid ischemic damage and enable successful replantation. The current inability to meet this challenge often leads to traumatic limb loss, which has a considerable detrimental impact on the quality of life of patients. METHODS: The authors' team built a portable extracorporeal membrane oxygenator device for the perfusion of amputated extremities with oxygenated acellular solution under controlled parameters...
April 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28340705/towards-automation-in-protein-digestion-development-of-a-monolithic-trypsin-immobilized-reactor-for-highly-efficient-on-line-digestion-and-analysis
#14
Marina Naldi, Urh Černigoj, Ales Štrancar, Manuela Bartolini
Reducing experimental variability, limiting contamination and increasing automation are essential goals in the development of reliable analytical platforms for mass spectrometry (MS)-based proteomics. In this work novel trypsin-based monolithic immobilized enzyme reactors (tryp-IMERs), obtained by covalent immobilization on convective interaction media (CIMac™) analytical columns (5mm×5.2mm I.D.), were developed. Notwithstanding the small dimensions, column format allowed the insertion in common high performance liquid chromatography (HPLC) systems, thus avoiding the use of expensive micro- or nano-platforms...
May 15, 2017: Talanta
https://www.readbyqxmd.com/read/28340453/long-term-nitrogen-deposition-increases-heathland-carbon-sequestration
#15
Chris D Field, Chris D Evans, Nancy B Dise, Jane R Hall, Simon J M Caporn
The large increases in reactive nitrogen (N) deposition in developed countries since the Industrial Revolution have had a marked impact on ecosystem functioning, including declining species richness, shifts in species composition, and increased N leaching. A potential mitigation of these harmful effects is the action of N as a fertiliser, which, through increasing primary productivity (and subsequently, organic matter production), has the potential to increase ecosystem carbon (C) storage. Here we report the response of an upland heath to 10years of experimental N addition...
March 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28338989/a-simple-stable-carbon-isotope-method-for-investigating-changes-in-the-use-of-recent-versus-old-carbon-in-oak
#16
Danny McCarroll, Matthew Whitney, Giles H F Young, Neil J Loader, Mary H Gagen
Stable carbon isotope ratios from early-wood (EW) and late-wood (LW) are used to test competing models of carbon storage and allocation, providing a cost-effective alternative to measuring and dating non-structural carbohydrates in mature temperate broad-leaf forest trees growing under natural conditions. Annual samples of EW and LW from seven mature oaks (Quercus robur L.) from Scotland, covering AD 1924-2012, were pooled, treated to isolate alpha-cellulose and pyrolysed to measure the carbon isotope ratios...
March 18, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28322747/quantitation-of-the-rates-of-hepatic-and-intestinal-cholesterol-synthesis-in-lysosomal-acid-lipase-deficient-mice-before-and-during-treatment-with-ezetimibe
#17
Jen-Chieh Chuang, Adam M Lopez, Stephen D Turley
Esterified cholesterol (EC) and triglycerides, contained within lipoproteins taken up by cells, are hydrolysed by lysosomal acid lipase (LAL) in the late endosomal/lysosomal (E/L) compartment. The resulting unesterified cholesterol (UC) is transported via Niemann-Pick type C2 and C1 into the cytosolic compartment where it enters a putative pool of metabolically active cholesterol that is utilized in accordance with cellular needs. Loss-of-function mutations in LIPA, the gene encoding LAL, result in dramatic increases in tissue concentrations of EC, a hallmark feature of Wolman disease and cholesteryl ester storage disease (CESD)...
March 18, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28314904/the-arabidopsis-orange-ator-gene-promotes-carotenoid-accumulation-in-transgenic-corn-hybrids-derived-from-parental-lines-with-limited-carotenoid-pools
#18
Judit Berman, Uxue Zorrilla-López, Vicente Medina, Gemma Farré, Gerhard Sandmann, Teresa Capell, Paul Christou, Changfu Zhu
The AtOR gene enhances carotenoid levels in corn by promoting the formation of plastoglobuli when the carotenoid pool is limited, but has no further effect when carotenoids are already abundant. The cauliflower orange (or) gene mutation influences carotenoid accumulation in plants by promoting the transition of proplastids into chromoplasts, thus creating intracellular storage compartments that act as metabolic sink. We overexpressed the Arabidopsis OR gene under the control of the endosperm-specific wheat LMW glutenin promoter in a white corn variety that normally accumulates only trace amounts of carotenoids...
March 17, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28268199/role-of-leishmanial-acidocalcisomal-pyrophosphatase-in-the-camp-homeostasis-in-phagolysosome-conditions-required-for-intra-macrophage-survival
#19
Arunima Biswas, Arijit Bhattacharya, Amit Vij, Pijush K Das
Exposure of Leishmania donovani to macrophage phagolysosome conditions (PC) (37°C and pH 5.5) led to increased intracellular cAMP and cAMP-mediated responses, which help in intra-macrophage survival pre-requisite for infectivity. In the absence of typical orthologs for G-proteins and G-protein coupled receptors, we sought to study the precise mechanisms for positive modulation of cAMP production during exposure to PC. Amongst two promastigote-stage specific membrane bound receptor adenylate cyclases (LdRAC-A and LdRAC-B), LdRAC-A appeared to function as a major cAMP generator following PC exposure...
March 6, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28263176/enhancing-uniformity-and-overall-quality-of-red-cell-concentrate-with-anaerobic-storage
#20
Tatsuro Yoshida, Abbejane Blair, Angelo D'alessandro, Travis Nemkov, Michael Dioguardi, Christopher C Silliman, Andrew Dunham
BACKGROUND: Recent research focused on understanding stored red blood cell (RBC) quality has demonstrated high variability in measures of RBC function and health across units. Studies have historically linked this high variability to variations in processing, storage method, and age. More recently, a large number of studies have focused on differences in donor demographics, donor iron sufficiency, and genetic predisposition of the donor to poor storage, particularly through mechanisms of accelerated oxidative damage...
March 2017: Blood Transfusion, Trasfusione del Sangue
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