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https://www.readbyqxmd.com/read/29781987/retrospective-microrna-sequencing-complementary-dna-library-preparation-protocol-using-formalin-fixed-paraffin-embedded-rna-specimens
#1
Olivier Loudig, Christina Liu, Thomas Rohan, Iddo Z Ben-Dov
-Archived, clinically classified formalin-fixed paraffin-embedded (FFPE) tissues can provide nucleic acids for retrospective molecular studies of cancer development. By using non-invasive or pre-malignant lesions from patients who later develop invasive disease, gene expression analyses may help identify early molecular alterations that predispose to cancer risk. It has been well described that nucleic acids recovered from FFPE tissues have undergone severe physical damage and chemical modifications, which make their analysis difficult and generally requires adapted assays...
May 5, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29780173/land-use-in-mountain-grasslands-alters-drought-response-and-recovery-of-carbon-allocation-and-plant-microbial-interactions
#2
Stefan Karlowsky, Angela Augusti, Johannes Ingrisch, Roland Hasibeder, Markus Lange, Sandra Lavorel, Michael Bahn, Gerd Gleixner
Mountain grasslands have recently been exposed to substantial changes in land use and climate and in the near future will likely face an increased frequency of extreme droughts. To date, how the drought responses of carbon (C) allocation, a key process in the C cycle, are affected by land-use changes in mountain grassland is not known.We performed an experimental summer drought on an abandoned grassland and a traditionally managed hay meadow and traced the fate of recent assimilates through the plant-soil continuum...
May 2018: Journal of Ecology
https://www.readbyqxmd.com/read/29775996/interplay-between-adenylate-metabolizing-enzymes-and-amp-activated-protein-kinase
#3
REVIEW
Marcella Camici, Simone Allegrini, Maria Grazia Tozzi
Purine nucleotides are involved in a variety of cellular functions, such as energy storage and transfer, and signalling, in addition to being the precursors of nucleic acids and cofactors of many biochemical reactions. They can be generated through two separate pathways, the de novo biosynthesis pathway and the salvage pathway. De novo purine biosynthesis leads to the formation of IMP, from which the adenylate and guanylate pools are generated by two additional steps. The salvage pathways utilize hypoxanthine, guanine and adenine to generate the corresponding mononucleotides...
May 18, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29768045/mfe-hi-adipose-tissue-macrophages-compensate-for-tissue-iron-pertubations-in-mice
#4
Merla J Hubler, Keith M Erikson, Arion J Kennedy, Alyssa H Hasty
Resident adipose tissue macrophages (ATMs) play multiple roles to maintain tissue homeostasis, such as removing excess FFAs and regulation of extracellular matrix. The phagocytic nature and oxidative resiliency of macrophages not only allows them to function as innate immune cells but also to respond to specific tissue needs, such as iron homeostasis. MFehi ATMs are a subtype of resident ATMs that we recently identified to have twice the intracellular iron content as other ATMs and elevated expression of iron handling genes...
May 16, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29752642/can-short-term-pasture-management-increase-c-balance-in-the-atlantic-rainforest
#5
Paulo Roberto da Rocha Junior, Felipe Vaz Andrade, Lucas Santos Satiro, Guilherme Kangussú Donagemma, Eduardo de Sá Mendonça
Few studies have shown the importance of different pasture management practices on C storage and the reduction of CO2 -C emissions in tropical conditions. The objective of the present study was to determine short-term changes in C pools and C balance from different pasture management practices established in the Atlantic Rainforest. A field study was carried out in Alegre, ES, Brazil from September 2013 to August 2014 to investigate the first-year effect of pasture management practices on a Udult clayey soil...
May 11, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29748989/shifts-in-priming-partly-explain-impacts-of-long-term-nitrogen-input-in-different-chemical-forms-on-soil-organic-carbon-storage
#6
Minghua Song, Yu Guo, Feihai Yu, Xianzhou Zhang, Guangmin Cao, Johannes H C Cornelissen
Input of labile organic carbon can enhance decomposition of extant soil organic carbon (SOC) through priming. We hypothesized that long-term nitrogen (N) input in different chemical forms alters SOC pools by altering priming effects associated with N-mediated changes in plants and soil microbes. The hypothesis was tested by integrating field experimental data of plants, soil microbes and two incubation experiments with soils that had experienced 10 years of N enrichment with three chemical forms (ammonium, nitrate and both ammonium and nitrate) in an alpine meadow on the Tibetan Plateau...
May 10, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29743075/clinical-grade-generation-of-peptide-stimulated-cmv-ebv-specific-t-cells-from-g-csf-mobilized-stem-cell-grafts
#7
Regina Gary, Michael Aigner, Stephanie Moi, Stefanie Schaffer, Anja Gottmann, Stefanie Maas, Robert Zimmermann, Jürgen Zingsem, Julian Strobel, Andreas Mackensen, Josef Mautner, Andreas Moosmann, Armin Gerbitz
BACKGROUND: A major complication after allogeneic hematopoietic stem cell transplantation (aSCT) is the reactivation of herpesviruses such as cytomegalovirus (CMV) and Epstein-Barr virus (EBV). Both viruses cause significant mortality and compromise quality of life after aSCT. Preventive transfer of virus-specific T cells can suppress reactivation by re-establishing functional antiviral immune responses in immunocompromised hosts. METHODS: We have developed a good manufacturing practice protocol to generate CMV/EBV-peptide-stimulated T cells from leukapheresis products of G-CSF mobilized and non-mobilized donors...
May 9, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29736197/xenomonitoring-of-mosquitoes-diptera-culicidae-for-the-presence-of-filarioid-helminths-in-eastern-austria
#8
Sarah Susanne Übleis, Claudia Cuk, Michaela Nawratil, Julia Butter, Ellen Schoener, Adelheid G Obwaller, Thomas Zechmeister, Georg G Duscher, Franz Rubel, Karin Lebl, Carina Zittra, Hans-Peter Fuehrer
Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 2013 and 2015, using different sampling techniques and storage conditions, and were analysed in pools with molecular tools for the presence of filarioid helminth DNA. Overall, DNA of D. repens , Setaria tundra , and two unknown filarioid helminths were documented in twenty mosquito pools within the mitochondrial cox1 gene (barcode region)...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29717178/volatile-diterpene-emission-by-two-mediterranean-cistaceae-shrubs
#9
A M Yáñez-Serrano, L Fasbender, J Kreuzwieser, D Dubbert, S Haberstroh, R Lobo-do-Vale, M C Caldeira, C Werner
Mediterranean vegetation emits a wide range of biogenic volatile organic compounds (BVOCs) among which isoprenoids present quantitatively the most important compound class. Here, we investigated the isoprenoid emission from two Mediterranean Cistaceae shrubs, Halimium halimifolium and Cistus ladanifer, under controlled and natural conditions, respectively. For the first time, diurnal emission patterns of the diterpene kaurene were detected in real-time by Proton-Transfer-Reaction-Time-of-Flight-Mass-Spectrometer...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29704660/genetic-and-epigenetic-control-of-adipose-development
#10
REVIEW
Olga Gulyaeva, Jon Dempersmier, Hei Sook Sul
White adipose tissue (WAT) is the primary energy storage organ and its excess contributes to obesity, while brown adipose tissue (BAT) and inducible thermogenic (beige/brite) adipocytes in WAT dissipate energy via Ucp1 to maintain body temperature. BAT and subcutaneous WAT develop perinatally while visceral WAT forms after birth from precursors expressing distinct markers, such as Myf5, Pref-1, Wt1, and Prx1, depending on the anatomical location. In addition to the embryonic adipose precursors, a pool of endothelial cells or mural cells expressing Pparγ, Pdgfrβ, Sma and Zfp423 may become adipocytes during WAT expansion in adults...
April 25, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29692554/seed-protein-fraction-electrophoresis-in-peanut-arachis-hypogaea-l-accessions-and-wild-species
#11
Apekshita Singh, Soom Nath Raina, Vijay Rani Rajpal, Anurudh K Singh
Total seed storage proteins were studied in 50 accessions of A. hypogaea (11 A. hypogaea ssp. hypogaea var hypogaea , 13 A. hypogaea ssp. hypogaea var hirsuta , 11 A. hypogaea ssp. fastigiata var fastigiata and 15 A. hypogaea ssp. fastigiata var. vulgaris accessions) in SDS PAGE. These accessions were also analysed for albumin and globulin seed protein fractions. Among the six seed protein markers presently used, it was found that globulin fraction showed maximum diversity (77.2%) in A. hypogaea accessions followed by albumin (52...
May 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29692127/-black-carbon-content-and-distribution-in-different-particle-size-fractions-of-forest-soils-in-the-middle-part-of-great-xing-an-mountains-china
#12
Jia Hui Xu, Lei Gao, Xiao Yang Cui
Soil black carbon (BC) is considered to be the main component of passive C pool because of its inherent biochemical recalcitrance. In this paper, soil BC in the middle part of Great Xing'an Mountains was quantified, the distribution of BC in different particle size fractions was analyzed, and BC stabilization mechanism and its important role in soil C pool were discussed. The results showed that BC expressed obvious accumulation in surface soil, accounting for about 68.7% in the whole horizon (64 cm), and then decreased with the increasing soil depth, however, BC/OC showed an opposite pattern...
October 2017: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29692033/-spatial-interpolation-model-of-soil-organic-carbon-density-considering-land-use-and-spatial-heterogeneity
#13
Zi Hao Wu, Yan Fang Liu, Yi Yun Chen, Long Guo, Qing Hu Jiang, Shao Chen Wang
Soil organic carbon pool is an important component of terrestrial carbon pool. Soil organic carbon pool and its dynamic change have important influence on carbon cycle in terrestrial ecosystem. Soil organic carbon density (SOCD) is an important parameter of soil carbon storage, and it is also an important index to evaluate farmland soil quality. Accurate prediction of regional organic carbon density spatial distribution is of great significance to the development of precision agriculture. A total of 242 farmland soil samples collected from the Jianghan Plain were used to explore the effects of land use types on the spatial distribution of SOCD in plain areas...
January 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29687892/metabolomics-evaluation-of-early-storage-red-blood-cell-rejuvenation-at-4%C3%A2-c-and-37%C3%A2-c
#14
Sarah Gehrke, Amudan J Srinivasan, Rachel Culp-Hill, Julie A Reisz, Andrea Ansari, Alan Gray, Matthew Landrigan, Ian Welsby, Angelo D'Alessandro
BACKGROUND: Refrigerated red blood cell (RBC) storage results in the progressive accumulation of biochemical and morphological alterations collectively referred to as the storage lesion. Storage-induced metabolic alterations can be in part reversed by rejuvenation practices. However, rejuvenation requires an incubation step of RBCs for 1 hour at 37°C, limiting the practicality of providing "on-demand," rejuvenated RBCs. We tested the hypothesis that the addition of rejuvenation solution early in storage as an adjunct additive solution would prevent-in a time window consistent with the average age of units transfused to sickle cell recipients at Duke (15 days)-many of the adverse biochemical changes that can be reversed via standard rejuvenation, while obviating the incubation step...
April 24, 2018: Transfusion
https://www.readbyqxmd.com/read/29686301/endocannabinoids-in-caenorhabditis-elegans-are-essential-for-the-mobilization-of-cholesterol-from-internal-reserves
#15
Celina Galles, Gastón M Prez, Sider Penkov, Sebastian Boland, Exequiel O J Porta, Silvia G Altabe, Guillermo R Labadie, Ulrike Schmidt, Hans-Joachim Knölker, Teymuras V Kurzchalia, Diego de Mendoza
Proper cholesterol transport is crucial for the functionality of cells. In C. elegans, certain cholesterol derivatives called dafachronic acids (DAs) govern the entry into diapause. In their absence, worms form a developmentally arrested dauer larva. Thus, cholesterol transport to appropriate places for DA biosynthesis warrants the reproductive growth. Recently, we discovered a novel class of glycosphingolipids, PEGCs, required for cholesterol mobilization/transport from internal storage pools. Here, we identify other components involved in this process...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29673841/electrospun-silk-fibroin-fibers-for-storage-and-controlled-release-of-human-platelet-lysate
#16
Cataldo Pignatelli, Giovanni Perotto, Marta Nardini, Ranieri Cancedda, Maddalena Mastrogiacomo, Athanassia Athanassiou
Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration of different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption has been limited because of its rapid degradation at room temperature that decreases its functionality. Another limiting factor for its extensive use is the difficulty of handling the hPL gels. In this work, silk fibroin-based patches were developed to address several points: improving the handling of hPL, enabling their delivery in a controlled manner and facilitating their storage by creating a device ready to use with expanded shelf life...
April 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29669007/storage-nitrogen-coordinates-leaf-expansion-and-photosynthetic-capacity-in-winter-oilseed-rape-brassica-napus-l
#17
Tao Liu, Tao Ren, Philip J White, Rihuan Cong, Jianwei Lu
Storage nitrogen (N) is a buffer pool for maintaining leaf growth and synthesizing photosynthetic proteins, but the dynamic of its forms within the life cycle of a single leaf and how it is influenced by N supply remain incompletely understood. A field experiment was conducted to estimate the influence of N supply on leaf growth, photosynthetic characteristics and N partitioning within the sixth leaves of winter oilseed rape (Brassica napus L.) from emergence through senescence. Storage N content (Nstore) decreased gradually along with leaf expansion...
April 12, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29666218/modelling-phosphorus-uptake-in-microalgae
#18
REVIEW
Dipali Singh, Ladislav Nedbal, Oliver Ebenhöh
Phosphorus (P) is an essential non-renewable nutrient that frequently limits plant growth. It is the foundation of modern agriculture and, to a large extent, demand for P is met from phosphate rock deposits which are limited and becoming increasingly scarce. Adding an extra stroke to this already desolate picture is the fact that a high percentage of P, through agricultural runoff and waste, makes its way into rivers and oceans leading to eutrophication and collapse of ecosystems. Therefore, there is a critical need to practise P recovery from waste and establish a circular economy applicable to P resources...
April 17, 2018: Biochemical Society Transactions
https://www.readbyqxmd.com/read/29644730/in-vitro-quality-of-apheresis-platelets-divided-into-paediatric-sized-units-and-stored-in-pvc-bags-plasticised-with-totm-bthc-or-dinch
#19
S Bashir, A Meli, R Cardigan
BACKGROUND AND OBJECTIVES: Two of the predictive factors of the quality of small volumes of platelets suitable for paediatric use are bag size and material. This study evaluated the storage properties of paediatric platelet aliquots in TOTM-, BTHC- or DINCH-PVC bags. METHODS/MATERIALS: (i) Three apheresis platelet concentrates (PC) were pooled and split into three units. One was retained as an adult unit (control; polyolefin bag). The second and third units were split into four MacoPharma TOTM-PVC and BTHC-PVC paediatric bags, respectively...
April 11, 2018: Transfusion Medicine
https://www.readbyqxmd.com/read/29624005/effects-of-seminal-plasma-concentration-on-sperm-motility-and-plasma-and-acrosome-membrane-integrity-in-chilled-canine-spermatozoa
#20
C Pan, Y Wu, Q Yang, J Ye
Depending on the mammal species, the use of seminal plasma during semen processing for cryopreservation has been found to have both beneficial and detrimental effects. This study was designed to determine the effects of seminal plasma concentration on the motility, sperm movement characteristics, and plasma and acrosome membrane integrity of chilled canine spermatozoa. After pooling the semen from seven dogs, samples for each assay were preserved at 4oC for 96h in extenders containing different seminal plasma concentrations (0, 25, 50, 75 and 100% (v/v) seminal plasma)...
March 2018: Polish Journal of Veterinary Sciences
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