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https://www.readbyqxmd.com/read/27927845/embryo-donation-and-understanding-of-kinship-the-impact-of-law-and-policy
#1
Jenni Millbank, Anita Stuhmcke, Isabel Karpin
STUDY QUESTION: What is the impact of law and policy upon the experience of embryo donation for reproductive use? SUMMARY ANSWER: Access to, and experience of, embryo donation are influenced by a number of external factors including laws that impose embryo storage limits, those that frame counselling and approval requirements and allow for, or mandate, donor identity disclosure. WHAT IS KNOWN ALREADY: To date only three qualitative studies in Australia and New Zealand have been completed on the experience of embryo donation for reproductive purposes, each with a small cohort of interviewees and divergent findings...
December 6, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27919600/preanalytic-of-total-antioxidant-capacity-assays-performed-in-serum-plasma-urine-and-saliva
#2
REVIEW
Cristiano Ialongo
The investigation of oxidative stress (OS), its mechanisms and connections with human diseases, is a topic of interest with more than 36,000 PubMed citations to date. The OS can be approached either from the perspective of pro-oxidation, either of anti-oxidation, and both can be investigated considering individual chemical constituents or their pooled effect. Actually, as it is for any laboratory assay, whatever source of variability introduces a bias potentially undermining the test results regardless of its application...
December 2, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27892539/chemotypic-variation-in-terpenes-emitted-from-storage-pools-influences-early-aphid-colonisation-on-tansy
#3
Mary V Clancy, Sharon E Zytynska, Matthias Senft, Wolfgang W Weisser, Jörg-Peter Schnitzler
Tansy plants (Tanacetum vulgare L.) exhibit high chemical variation, particularly in mono- and sesquiterpenes that are stored in specialised glands on the plant surface. In the present work we investigated the effects of terpene chemotypes on Metopeurum fuscoviride, an aphid species specialised on tansy, and their tending ants, at the field scale. Previous studies have chemotyped tansy by assessing dominant compounds; here we propose a method of chemotyping using all volatile compounds that are likely emitted from the storage glands...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888692/mitochondrial-gsh-replenishment-as-a-potential-therapeutic-approach-for-niemann-pick-type-c-disease
#4
Sandra Torres, Nuria Matías, Anna Baulies, Susana Nuñez, Cristina Alarcon-Vila, Laura Martinez, Natalia Nuño, Anna Fernandez, Joan Caballeria, Thierry Levade, Alba Gonzalez-Franquesa, Pablo Garcia-Rovés, Elisa Balboa, Silvana Zanlungo, Gemma Fabrías, Josefina Casas, Carlos Enrich, Carmen Garcia-Ruiz, José C Fernández-Checa
Niemann Pick type C (NPC) disease is a progressive lysosomal storage disorder caused by mutations in genes encoding NPC1/NPC2 proteins, characterized by neurological defects, hepatosplenomegaly and premature death. While the primary biochemical feature of NPC disease is the intracellular accumulation of cholesterol and gangliosides, predominantly in endolysosomes, mitochondrial cholesterol accumulation has also been reported. As accumulation of cholesterol in mitochondria is known to impair the transport of GSH into mitochondria, resulting in mitochondrial GSH (mGSH) depletion, we investigated the impact of mGSH recovery in NPC disease...
November 20, 2016: Redox Biology
https://www.readbyqxmd.com/read/27888380/kinetin-improves-motility-viability-and-antioxidative-parameters-of-ram-semen-during-storage-at-refrigerator-temperature
#5
Elham Zadeh Hashem, Mohsen Eslami
The present experiment was conducted to evaluate the effect of kinetin on ram semen quality during cold storage. Ejaculates were collected using an artificial vagina from five Qezel rams. Ejaculates which met the criteria (volume of 0.75-2 ml; minimum spermatozoa concentration of 2.5 × 109 spermatozoa/ml and forward progressive motility of 80%), were pooled, diluted with extender without kinetin (control) or enriched with 25 (K 25), 50 (K 50), 100 (K 100) and 200 (K 200) μM kinetin at a final concentration of 500 × 106 spermatozoa per mL...
November 25, 2016: Cell and Tissue Banking
https://www.readbyqxmd.com/read/27882572/a-paired-comparison-of-thawed-and-liquid-plasma
#6
Lucy Backholer, Laura Green, Sian Huish, Sean Platton, Michael Wiltshire, Heidi Doughty, Elinor Curnow, Rebecca Cardigan
BACKGROUND: To make plasma readily available to treat major hemorrhage, some centers are internationally using either thawed plasma (TP) or "never-frozen" liquid plasma (LP). Despite the routine use of both, there are limited data comparing the two. The hemostatic properties of LP were evaluated and compared to TP in a paired study. STUDY DESIGN AND METHODS: Two ABO-matched plasma units were pooled and split to produce 1 unit for LP and 1 unit for TP. Samples of TP and LP, stored at 2 to 6°C, were tested for a range of coagulation factors, thrombin generation, and rotational thromboelastometry...
November 23, 2016: Transfusion
https://www.readbyqxmd.com/read/27865047/carbon-lost-and-carbon-gained-a-study-of-vegetation-and-carbon-tradeoffs-among-diverse-land-uses-in-the-phoenix-az
#7
Melissa R McHale, Sharon Hall, Anandamayee Majumdar, Nancy B Grimm
Human modification and management of urban landscapes drastically alters vegetation and soils, thereby altering carbon (C) storage and rates of net primary productivity (NPP). Complex social and ecological processes drive vegetation cover in cities, leading to heterogeneity in C dynamics depending on regional climate, land use, and land cover. Recent work has demonstrated homogenization in ecological processes within human-dominated landscapes (the urban convergence hypothesis) in soils and biotic communities...
November 19, 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27860296/impact-of-subnormothermic-machine-perfusion-preservation-in-severely-steatotic-rat-livers-a-detailed-assessment-in-an-isolated-setting
#8
Y Okamura, K Hata, H Tanaka, H Hirao, T Kubota, O Inamoto, S Kageyama, I Tamaki, N Yermek, J Yoshikawa, S Uemoto
Current drastic shortage of donor organs has led to acceptance of extended-criteria donors for transplantation, despite higher risk of primary non-function. Here, we report the impact of subnormothermic machine perfusion (SMP) preservation on the protection of >50% macro-steatotic livers. Dietary hepatic steatosis was induced in Wistar rats via 2-day fasting and subsequent 3-day re-feeding with a fat-free, carbohydrate-rich diet. This protocol induces 50-60% macrovesicular steatosis, which should be discarded when preserved via cold storage (CS)...
November 10, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27859095/controls-on-carbon-storage-and-weathering-in-volcanic-soils-across-a-high-elevation-climate-gradient-on-mauna-kea-hawaii
#9
Marc G Kramer, Oliver A Chadwick
Volcanic ash soils retain the largest and most persistent soil carbon pools of any ecosystem. However, the mechanisms governing soil carbon accumulation and weathering during initial phases of ecosystem development are not well understood. We examined soil organic matter dynamics and soil development across a high-altitude (3,560-3,030 m) 20-kyr climate gradient on Mauna Kea in Hawaii. Four elevation sites were selected (~250-500 mm rainfall), which range from sparsely vegetated to sites that contain a mix of shrubs and grasses...
September 2016: Ecology
https://www.readbyqxmd.com/read/27849413/storage-pool-diseases-illuminate-platelet-dense-granule-biogenesis
#10
Andrea L Ambrosio, Santiago M Di Pietro
Platelet dense granules (DGs) are membrane bound compartments that store polyphosphate and small molecules such as ADP, ATP, Ca(2+), and serotonin. The release of DG contents plays a central role in platelet aggregation to form a hemostatic plug. Accordingly, congenital deficiencies in the biogenesis of platelet DGs underlie human genetic disorders that cause storage pool disease and manifest with prolonged bleeding. DGs belong to a family of lysosome-related organelles, which also includes melanosomes, the compartments where the melanin pigments are synthesized...
November 16, 2016: Platelets
https://www.readbyqxmd.com/read/27847866/carbon-emissions-from-land-use-change-and-management-in-china-between-1990-and-2010
#11
Li Lai, Xianjin Huang, Hong Yang, Xiaowei Chuai, Mei Zhang, Taiyang Zhong, Zhigang Chen, Yi Chen, Xiao Wang, Julian R Thompson
China has experienced enormous changes in land use in recent decades, which are largely driven by its unparalleled economic development. We analyze changes in vegetation and soil carbon storage between 1990 and 2010 resulting from combinations of land-use category conversion and management. Results demonstrate a major decline in grasslands (-6.85%; 20.83 × 10(6) ha) and large increases in urban areas (+43.73%; 6.87 × 10(6) ha), farmlands (+0.84%; 1.48 × 10(6) ha), and forests (+0.67%; 1.52 × 10(6) ha). The total soil organic carbon pool has been reduced by approximately 11...
November 2016: Science Advances
https://www.readbyqxmd.com/read/27837413/machine-perfusion-versus-cold-storage-of-livers-a-meta-analysis
#12
Sushun Liu, Qing Pang, Jingyao Zhang, Mimi Zhai, Sinan Liu, Chang Liu
Different organ preservation methods are key factors influencing the results of liver transplantation. In this study, the outcomes of experimental models receiving donation after cardiac death (DCD) livers preserved through machine perfusion (MP) or static cold storage (CS) were compared by conducting a meta-analysis. Standardized mean difference (SMD) and 95% confidence interval (CI) were calculated to compare pooled data from two animal species. Twenty-four studies involving MP preservation were included in the meta-analysis...
November 11, 2016: Frontiers of Medicine
https://www.readbyqxmd.com/read/27830502/vitamin-a-absorption-storage-and-mobilization
#13
William S Blaner, Yang Li, Pierre-Jacques Brun, Jason J Yuen, Seung-Ah Lee, Robin D Clugston
It is well established that chylomicron remnant (dietary) vitamin A is taken up from the circulation by hepatocytes, but more than 80 % of the vitamin A in the liver is stored in hepatic stellate cells (HSC). It presently is not known how vitamin A is transferred from hepatocytes to HSCs for storage. Since retinol-binding protein 4 (RBP4), a protein that is required for mobilizing stored vitamin A, is synthesized solely by hepatocytes and not HSCs, it similarly is not known how vitamin A is transferred from HSCs to hepatocytes...
2016: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/27829182/benchmarking-criticality-analysis-of-triga-fuel-storage-racks
#14
Matthew Loren Robinson, Timothy M DeBey, Jack F Higginbotham
A criticality analysis was benchmarked to sub-criticality measurements of the hexagonal fuel storage racks at the United States Geological Survey TRIGA MARK I reactor in Denver. These racks, which hold up to 19 fuel elements each, are arranged at 0.61m (2 feet) spacings around the outer edge of the reactor. A 3-dimensional model was created of the racks using MCNP5, and the model was verified experimentally by comparison to measured subcritical multiplication data collected in an approach to critical loading of two of the racks...
January 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/27818535/amine-reactive-dyes-an-alternative-to-estimate-membrane-integrity-in-fish-sperm-cells
#15
Leticia Torres, Terrence R Tiersch
Fluorescent dyes that binds irreversibly to cellular amines, come in several available emission spectra, and do not poses health concerns were used to evaluate membrane integrity in fish sperm cells. The objectives of the present study were to determine: (1) a working dye concentration for fish sperm samples, and (2) if the traditional propidium iodide/SYBR-14 staining combination was comparable with the amine reactive dye (ARD) methods at identifying cell populations with intact and compromised membranes after sperm activation, refrigerated storage, and exposure to cryoprotectant and surfactant...
October 1, 2016: Aquaculture
https://www.readbyqxmd.com/read/27789059/protective-effects-of-opuntia-ficus-indica-extract-on-ram-sperm-quality-lipid-peroxidation-and-dna-fragmentation-during-liquid-storage
#16
Larbi Allai, Xavier Druart, Mehmet Öztürk, Anass BenMoula, Boubker Nasser, Bouchra El Amiri
The present study aimed to assess the phenolic composition of the acetone extract from Opuntia ficus indica cladodes (ACTEX) and its effects on ram semen variables, lipid peroxidation and DNA fragmentation during liquid storage at 5°C for up to 72h in skim milk and Tris egg yolk extenders. Semen samples from five rams were pooled extended with Tris-egg yolk (TEY) or skim milk (SM) extenders containing ACTEX (0%, 1%, 2%, 4% and 8%) at a final concentration of 0.8×10(9) sperm/ml and stored for up to 72h at 5°C...
December 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27783454/successful-donation-after-cardiac-death-liver-transplants-with-prolonged-warm-ischemia-time-using-normothermic-regional-perfusion
#17
R De Carlis, S Di Sandro, A Lauterio, F Ferla, A Dell'Acqua, M Zanierato, L De Carlis
The role of donation after cardiac death (DCD) in expanding the donor pool is mainly limited by the incidence of primary non-function (PNF) and ischemia-related complications. Even greater concern exists towards uncontrolled DCD, which represents the largest potential pool of DCD donors. We recently started the first Italian series of DCD liver transplantation, using normothermic regional perfusion (NRP) in 6 uncontrolled donors and in 1 controlled case to deal with the legally required no-touch period of 20 minutes...
October 26, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/27783371/-new-insight-into-anopheles-coluzzii-coetzee-wilkerson-2013-larval-ecology-in-the-wouri-estuary-littoral-cameroon
#18
A Mbida Mbida, J Etang, P Akono Ntonga, C Eboumbou Moukoko, P Awono-Ambene, D Tagne, A Talipouo, W Ekoko, J Binyang, R Tchoffo, G Lehman, R Mimpfoundi
Malaria vectors control is essentially based on the use of insecticides against adult mosquitoes. However because of the development of resistance to insecticides, there is now a renewed interest in the management of larval sources. The aim of the present study was to map and characterize the breeding sites of Anopheles coluzzii in the Wouri river estuary in Cameroon. Larval surveys were carried out between December 2013 and August 2014 in rural areas on the island of Manoka and urban area in Youpwe at Douala...
October 25, 2016: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/27742886/oil-biosynthesis-in-underground-oil-rich-storage-vegetative-tissue-comparison-of-cyperus-esculentus-tuber-with-oil-seeds-and-fruits
#19
Zhenle Yang, Hongying Ji, Dantong Liu
Cyperus esculentus is unique in that it can accumulate rich oil in tuber. However, the underlying mechanism of tuber oil biosynthesis is still unclear. Our transcriptional analyses of the pathways from pyruvate production up to triacylglycerol (TAG) accumulation in tuber revealed many distinct species-specific lipid expression patterns from oil seeds and fruits, indicating that in C. esculentus tuber, (1) carbon flux from sucrose toward plastid pyruvate could be produced mostly through cytosolic glycolytic pathway, (2) acetyl-CoA synthetase might be an important contributor to acetyl-CoA formation for plastid fatty acid biosynthesis, (3) expression pattern for stearoyl-ACP desaturase was associated with high oleic acid composition, (4) it was most likely that endoplasmic reticulum-associated acyl-CoA synthetase played a significant role in the export of fatty acids between the plastid and ER, (5) lipid phosphate phosphatase (LPP)-δ was most probably related to the formation of DAG pool in Kennedy pathway, (6) diacylglyceroltransacylase 2 (DGAT2) and phospholipid:diacylglycerolacyltransferase 1 (PDAT1) might play crucial roles in tuber oil biosynthesis...
October 13, 2016: Plant & Cell Physiology
https://www.readbyqxmd.com/read/27742324/scanning-electron-microscopy-findings-of-machine-perfused-liver-graft-after-warm-ischemia-between-hypothermic-and-rewarming-machine-perfusion-in-pigs
#20
L Meng, N Matsuno, K Watanabe, M Furukori, H Obara, H Bochimoto, T Watanabe, H Fukukawa
BACKGROUND: The shortage of organ donors is a universal problem. Use of grafts from donors after cardiac death would greatly contribute to the expansion of the donor organ pool. The two major methods of preservation are cold storage and machine perfusion (MP) preservation, and each has its own advantages. Several studies have reported the relative merits of MP for the preservation for grafts from donors after cardiac death. In this study, we used scanning electron microscopy (SEM) to assess the damage to the liver between hypothermic and rewarming preservation conditions...
September 2016: Transplantation Proceedings
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