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https://www.readbyqxmd.com/read/28734349/integrated-preservation-and-sample-clean-up-procedures-for-studying-water-ingestion-by-recreational-swimmers-via-urinary-biomarker-determination
#1
Ricardo Cantú, Jody A Shoemaker, Catherine A Kelty, Larry J Wymer, Thomas D Behymer, Alfred P Dufour, Matthew L Magnuson
The use of cyanuric acid as a biomarker for ingestion of swimming pool water may lead to quantitative knowledge of the volume of water ingested during swimming, contributing to a better understanding of disease resulting from ingestion of environmental contaminants. When swimming pool water containing chlorinated cyanurates is inadvertently ingested, cyanuric acid is excreted quantitatively within 24 h as a urinary biomarker of ingestion. Because the volume of water ingested can be quantitatively estimated by calculation from the concentration of cyanuric acid in 24 h urine samples, a procedure for preservation, cleanup, and analysis of cyanuric acid was developed to meet the logistical demands of large scale studies...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28726794/biological-iron-sulfur-storage-in-a-thioferrate-protein-nanoparticle
#2
Brian J Vaccaro, Sonya M Clarkson, James F Holden, Dong-Woo Lee, Chang-Hao Wu, Farris L Poole Ii, Julien J H Cotelesage, Mark J Hackett, Sahel Mohebbi, Jingchuan Sun, Huilin Li, Michael K Johnson, Graham N George, Michael W W Adams
Iron-sulfur clusters are ubiquitous in biology and function in electron transfer and catalysis. They are assembled from iron and cysteine sulfur on protein scaffolds. Iron is typically stored as iron oxyhydroxide, ferrihydrite, encapsulated in 12 nm shells of ferritin, which buffers cellular iron availability. Here we have characterized IssA, a protein that stores iron and sulfur as thioferrate, an inorganic anionic polymer previously unknown in biology. IssA forms nanoparticles reaching 300 nm in diameter and is the largest natural metalloprotein complex known...
July 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28710452/factors-affecting-distribution-patterns-of-organic-carbon-in-sediments-at-regional-and-national-scales-in-china
#3
Qingqing Cao, Hui Wang, Yiran Zhang, Rattan Lal, Renqing Wang, Xiuli Ge, Jian Liu
Wetlands are an important carbon reservoir pool in terrestrial ecosystems. Light fraction organic carbon (LFOC), heavy fraction organic carbon (HFOC), and dissolved organic carbon (DOC) were fractionated in sediment samples from the four wetlands (ZR: Zhaoniu River; ZRCW: Zhaoniu River Constructed Wetland; XR: Xinxue River; XRCW: Xinxue River Constructed Wetland). Organic carbon (OC) from rivers and coasts of China were retrieved and statistically analyzed. At regional scale, HFOC stably dominates the deposition of OC (95...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28707260/historical-effects-of-dissolved-organic-carbon-export-and-land-management-decisions-on-the-watershed-scale-forest-carbon-budget-of-a-coastal-british-columbia-douglas-fir-dominated-landscape
#4
B P Smiley, J A Trofymow
BACKGROUND: To address how natural disturbance, forest harvest, and deforestation from reservoir creation affect landscape-level carbon (C) budgets, a retrospective C budget for the 8500 ha Sooke Lake Watershed (SLW) from 1911 to 2012 was developed using historical spatial inventory and disturbance data. To simulate forest C dynamics, data was input into a spatially-explicit version of the Carbon Budget Model-Canadian Forest Sector (CBM-CFS3). Transfers of terrestrial C to inland aquatic environments need to be considered to better capture the watershed scale C balance...
December 2017: Carbon Balance and Management
https://www.readbyqxmd.com/read/28706285/transcriptional-regulation-of-cellulose-biosynthesis-during-the-early-phase-of-nitrogen-deprivation-in-nannochloropsis-salina
#5
Seok Won Jeong, Seung Won Nam, Kwon HwangBo, Won Joong Jeong, Byeong-Ryool Jeong, Yong Keun Chang, Youn-Il Park
Microalgal photosynthesis provides energy and carbon-containing precursors for the biosynthesis of storage carbohydrates such as starch, chrysolaminarin, lipids, and cell wall components. Under mild nitrogen deficiency (N-), some Nannochloropsis species accumulate lipid by augmenting cytosolic fatty acid biosynthesis with a temporary increase in laminarin. Accordingly, biosynthesis of the cellulose-rich cell wall should change in response to N- stress because this biosynthetic pathway begins with utilisation of the hexose phosphate pool supplied from photosynthesis...
July 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28699207/supplemental-effect-of-different-levels-of-taurine-in-modena-on-boar-semen-quality-during-liquid-preservation-at-17%C3%A2-c
#6
Hao Li, Xiao-Gang Zhang, Qian Fang, Qi Liu, Ren-Rang Du, Gong-She Yang, Li-Qiang Wang, Jian-Hong Hu
Peroxidation damage induces sublethal injury to boar sperm during the storage process. Taurine has already been demonstrated to protect cells effectively from oxidant-induced injury. This study was aimed to evaluate the effect of different concentrations of taurine (0.5, 1, 5 and 10 mmol/L) in Modena diluent on boar sperm quality during liquid storage at 17°C. Ejaculates from sexually mature Duroc pigs were collected, pooled and preserved in the Modena containing different concentrations of taurine. Sperm motility, plasma membrane integrity, acrosome integrity, total antioxidative capacity (T-AOC) activity and malondialdehyde content (MDA) were examined every 24 h...
July 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28697783/validation-of-qtl-mapping-and-transcriptome-profiling-for-identification-of-candidate-genes-associated-with-nitrogen-stress-tolerance-in-sorghum
#7
Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E Clemente, David R Holding, Ismail M Dweikat
BACKGROUND: Quantitative trait loci (QTLs) detected in one mapping population may not be detected in other mapping populations at all the time. Therefore, before being used for marker assisted breeding, QTLs need to be validated in different environments and/or genetic backgrounds to rule out statistical anomalies. In this regard, we mapped the QTLs controlling various agronomic traits in a recombinant inbred line (RIL) population in response to Nitrogen (N) stress and validated these with the reported QTLs in our earlier study to find the stable and consistent QTLs across populations...
July 11, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28692131/vitis-vinifera-root-and-leaf-metabolic-composition-during-fruit-maturation-implications-of-defoliation
#8
Gerhard C Rossouw, Beverley A Orchard, Katja Šuklje, Jason P Smith, Celia Barril, Alain Deloire, Bruno P Holzapfel
Grapevine (Vitis vinifera) roots and leaves represent major carbohydrate and nitrogen (N) sources, either as recent assimilates, or mobilized from labile or storage pools. This study examined the response of root and leaf primary metabolism following defoliation treatments applied to fruiting vines during ripening. The objective was to link alterations in root and leaf metabolism to carbohydrate and N source functioning under conditions of increased fruit sink demand. Potted grapevine leaf area was adjusted near the start of véraison to 25 primary leaves per vine compared to 100 leaves for the control...
July 10, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28681326/evolvement-rules-of-basin-flood-risk-under-low-carbon-mode-part-i-response-of-soil-organic-carbon-to-land-use-change-and-its-influence-on-land-use-planning-in-the-haihe-basin
#9
Fawen Li, Liping Wang, Yong Zhao
Soil organic carbon (SOC) plays an important role in the global carbon cycle. The aim of this study was to evaluate the response of SOC to land use change and its influence on land use planning in the Haihe basin, and provide planning land use pattern for basin flood risk assessment. Firstly, the areas of different land use types in 1980, 2008, and the planning year (2020) were counted by area statistics function of ArcGIS. Then, the transfer matrixes of land use were produced by spatial overlay analysis function...
August 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28679747/bacterial-physiological-adaptations-to-contrasting-edaphic-conditions-identified-using-landscape-scale-metagenomics
#10
Ashish A Malik, Bruce C Thomson, Andrew S Whiteley, Mark Bailey, Robert I Griffiths
Environmental factors relating to soil pH are important regulators of bacterial taxonomic biodiversity, yet it remains unclear if such drivers affect community functional potential. To address this, we applied whole-genome metagenomics to eight geographically distributed soils at opposing ends of a landscape soil pH gradient (where "low-pH" is ~pH 4.3 and "high-pH" is ~pH 8.3) and evaluated functional differences with respect to functionally annotated genes. First, differences in taxonomic and functional diversity between the two pH categories were assessed with respect to alpha diversity (mean sample richness) and gamma diversity (total richness pooled for each pH category)...
July 5, 2017: MBio
https://www.readbyqxmd.com/read/28675672/multi-date-multi-sensor-remote-sensing-reveals-high-density-of-carbon-rich-mountain-peatlands-in-the-p%C3%A3-ramo-of-ecuador
#11
John A Hribljan, Esteban Suarez, Laura Bourgeau-Chavez, Sarah Endres, Erik A Lilleskov, Segundo Chimbolema, Craig Wayson, Eleanor Serocki, Rodney A Chimner
Tropical peatlands store a significant portion of the global soil carbon (C) pool. However, tropical mountain peatlands contain extensive peat soils that have yet to be mapped or included in global C estimates. This lack of data hinders our ability to inform policy and apply sustainable management practices to these peatlands that are experiencing unprecedented high rates of land use and land cover change. Rapid large scale mapping activities are urgently needed to quantify tropical wetland extent and rate of degradation...
July 4, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28666916/emerging-roles-for-conjugated-sterols-in-plants
#12
REVIEW
Albert Ferrer, Teresa Altabella, Montserrat Arró, Albert Boronat
In plants, sterols are found in free form (free sterols, FSs) and conjugated as steryl esters (SEs), steryl glycosides (SGs) and acyl steryl glycosides (ASGs). Conjugated sterols are ubiquitously found in plants but their relative contents highly differ among species and their profile may change in response to developmental and environmental cues. SEs play a central role in membrane sterol homeostasis and also represent a storage pool of sterols in particular plant tissues. SGs and ASGs are main components of the plant plasma membrane (PM) that specifically accumulate in lipid rafts, PM microdomains known to mediate many relevant cellular processes...
July 2017: Progress in Lipid Research
https://www.readbyqxmd.com/read/28653930/identification-and-growth-optimization-of-a-marine-bacillus-dk1-sa11-having-potential-of-producing-broad-spectrum-antimicrobial-compounds
#13
Muhammad Naseem Khan, Hong Lin, Meng Li, Jingxue Wang, Zulfiqar Ali Mirani, Seema Ismat Khan, Muhammad Aslam Buzdar, Imran Ali, Khalid Jamil
Control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. Marine bacteria are an enormous source of bio-controlling agents. The aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain DK1-SA11 isolated from Qingdao Bay of China Yellow Sea. Microscopic characterization, API® 20E and 50 CHB kit base carbohydrates utilization, 16S rDNA and DNA gyrB gene sequencing studies identified the bacterium as Bacillus subtilis subsp...
May 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28641229/effects-of-short-term-invasion-of-spartina-alterniflora-and-the-subsequent-restoration-of-native-mangroves-on-the-soil-organic-carbon-nitrogen-and-phosphorus-stock
#14
Jianxiang Feng, Jian Zhou, Liming Wang, Xiaowei Cui, Cunxin Ning, Hao Wu, Xiaoshan Zhu, Guanghui Lin
The exotic cordgrass Spartina alterniflora has severely invaded the mangrove wetlands in southern China and ecological restoration using native mangroves was conducted in an attempt to control this invasive species. In this study, the contents and pools of soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) were quantified to investigate the invasive effects of S. alterniflora and then to evaluate whether the ecological restoration of native mangrove could reverse those effects. S. alterniflora only showed significantly higher organic carbon content in the surface 0-10 cm of soil than in the uninvaded mudflat...
June 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28634352/differences-in-sequences-between-hbv-relaxed-circular-dna-and-covalently-closed-circular-dna
#15
Magda Rybicka, Anna Woziwodzka, Tomasz Romanowski, Piotr Stalke, Marcin Dręczewski, Krzysztof Piotr Bielawski
The hepatitis B virus (HBV) genome exists in two forms: circular covalently closed DNA (cccDNA) and relaxed circular DNA (RCDNA). Here, we investigated the presence of differences in the sequences of both forms in paired samples of serum and liver tissue. The serum and liver biopsy samples were collected at the same time from 67 chronically infected patients. The genotyping of the RCDNA and cccDNA was performed using mass spectrometry analysis. The HBV mutations located in the HBV pol (P) and the HBV basal core promoter/pre-core (BCP/PC) regions were included...
June 21, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28624109/the-use-of-old-donors-in-liver-transplantation
#16
REVIEW
Bobby V M Dasari, Andrea Schlegel, Hynek Mergental, M Thamara P R Perera
The process of ageing has an impact on the entire human body including the organ systems. In transplantation, professionals are daily faced with risk assessment of suitable donor offers , whether to accept a liver graft for a specific recipient. In this context, livers from elderly donors are more frequently accepted for transplantation, to increase the donor pool and compensate the high waiting list mortality. In the current practice it is not unusual to accept 60-year old donor livers for transplantation, as the donor demographics have significantly changed over the years...
April 2017: Best Practice & Research. Clinical Gastroenterology
https://www.readbyqxmd.com/read/28619669/sex-pheromone-in-the-moth-heliothis-virescens-is-produced-as-a-mixture-of-two-pools-de-novo-and-via-precursor-storage-in-glycerolipids
#17
Stephen P Foster, Karin G Anderson, Jérôme Casas
Most species of moths use a female-produced volatile sex pheromone, typically produced via de novo fatty acid synthesis in a specialized gland, for communication among mates. While de novo biosynthesis of pheromone (DNP) is rapid, suggesting transient precursor acids, substantial amounts of pheromone precursor (and other) acids are stored, predominantly in triacylglycerols in the pheromone gland. Whether these stored acids are converted to pheromone later or not has been the subject of some debate. Using a tracer/tracee approach, in which we fed female Heliothis virescens U-(13)C-glucose, we were able to distinguish two pools of pheromone, in which precursors were temporally separated (after and before feeding on labeled glucose): DNP synthesized from a mixed tracer/tracee acetyl CoA pool after feeding, and pheromone made from precursor acids primarily synthesized before feeding, which we call recycled precursor fat pheromone (RPP)...
June 12, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28618360/the-distribution-of-phosphorus-and-its-transformations-during-batch-growth-of-synechocystis
#18
Yun Zhou, Binh T Nguyen, Chen Zhou, Levi Straka, YenJung Sean Lai, Siqing Xia, Bruce E Rittmann
Phosphorus (P) is an essential nutrient that affects the growth and metabolism of microalgal biomass. Despite the obvious importance of P, the dynamics of how it is taken up and distributed in microalgae are largely undefined. In this study, we tracked the fate of P during batch growth of the cyanobacterium Synechocystis sp. PCC 6803. We determined the distribution of P in intracellular polymeric substances (IPS), extracellular polymeric substances (EPS), and soluble microbial products (SMP) for three initial ortho-phosphate concentrations...
June 9, 2017: Water Research
https://www.readbyqxmd.com/read/28618110/intravesical-vanilloids-for-treating-neurogenic-lower-urinary-tract-dysfunction-in-patients-with-multiple-sclerosis-a-systematic-review-and-meta-analysis-a-report-from-the-neuro-urology-promotion-committee-of-the-international-continence-society-ics
#19
REVIEW
Véronique Phé, Marc P Schneider, Benoit Peyronnet, Nadim Abo Youssef, Livio Mordasini, Emmanuel Chartier-Kastler, Lucas M Bachmann, Thomas M Kessler
AIMS: To systematically assess all available evidence on efficacy and safety of vanilloids for treating neurogenic lower urinary tract dysfunction (NLUTD) in patients with multiple sclerosis (MS). METHODS: This systematic review and meta-analysis was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Studies were identified by electronic search of Cochrane register, Embase, Medline, Scopus, (last search January 8, 2016)...
June 15, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28592692/genome-wide-temporal-expression-profiling-in-c-elegans-identifies-a-core-gene-set-related-to-long-term-memory
#20
Virginie Freytag, Sabine Probst, Nils Hadziselimovic, Csaba Boglari, Yannick Hauser, Fabian Peter, Bank Gabor Fenyves, Annette Milnik, Philippe Demougin, Vanja Vukojevic, Dominique J-F de Quervain, Andreas Papassotiropoulos, Attila Stetak
The identification of genes related to encoding, storage, and retrieval of memories is a major interest in neuroscience. In the current study, we analyzed the temporal gene expression changes in a neuronal mRNA pool during an olfactory long-term associative memory (LTAM) in C. elegans hermaphrodites. Here we identified a core set of 712 (538 upregulated and 174 downregulated) genes that follow three distinct temporal peaks demonstrating multiple gene regulation waves in LTAM. Comparing with the previously published positive LTAM gene sets (Lakhina et al...
June 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
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