keyword
MENU ▼
Read by QxMD icon Read
search

Storage pool

keyword
https://www.readbyqxmd.com/read/28096191/allosteric-modulation-of-the-substrate-specificity-of-acyl-coa-wax-alcohol-acyltransferase-2-awat2
#1
Jason M Arne, Made Airanthi K Widjaja-Adhi, Taylor Hughes, Kevin W Huynh, Josie A Silvaroli, Sylwia Chelstowska, Vera Y Moiseenkova-Bell, Marcin Golczak
The esterification of alcohols with fatty acids is a universal mechanism to form inert storage forms of sterols, di- and triacylglycerols, and retinoids. In ocular tissues, formation of retinyl esters is an essential step in the enzymatic regeneration of the visual chromophore (11-cis-retinal). Acyl-CoA wax alcohol acyltransferase 2 (AWAT2), also known as multifunctional O-acyltransferase (MFAT), is an integral membrane enzyme with a broad substrate specificity that has been shown to preferentially esterify 11-cis-retinol and thus contribute to formation of readily available pool of cis retinoids in the eye...
January 17, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28076405/development-and-validation-of-a-new-reliable-method-for-the-diagnosis-of-avian-botulism
#2
Caroline Le Maréchal, Sandra Rouxel, Valentine Ballan, Emmanuelle Houard, Typhaine Poezevara, Marie-Hélène Bayon-Auboyer, Rozenn Souillard, Hervé Morvan, Marie-Agnès Baudouard, Cédric Woudstra, Christelle Mazuet, Sophie Le Bouquin, Patrick Fach, Michel Popoff, Marianne Chemaly
Liver is a reliable matrix for laboratory confirmation of avian botulism using real-time PCR. Here, we developed, optimized, and validated the analytical steps preceding PCR to maximize the detection of Clostridium botulinum group III in avian liver. These pre-PCR steps included enrichment incubation of the whole liver (maximum 25 g) at 37°C for at least 24 h in an anaerobic chamber and DNA extraction using an enzymatic digestion step followed by a DNA purification step. Conditions of sample storage before analysis appear to have a strong effect on the detection of group III C...
2017: PloS One
https://www.readbyqxmd.com/read/28072862/efficacy-and-safety-of-initial-combination-treatment-of-an-alpha-blocker-with-an-anticholinergic-medication-in-benign-prostatic-hyperplasia-patients-with-lower-urinary-tract-symptoms-updated-meta-analysis
#3
Hyun Jung Kim, Hwa Yeon Sun, Hoon Choi, Jae Young Park, Jae Hyun Bae, Seung Whan Doo, Won Jae Yang, Yun Seob Song, Young Myoung Ko, Jae Heon Kim
BACKGROUND: There is still controversy as to whether initial combination treatment is superior to serial addition of anticholinergics after maintenance or induction of alpha blockers in benign prostatic hyperplasia (BPH)/lower urinary tract symptoms (LUTS). OBJECTIVE: The objective of this study was to determine the benefits and safety of initial combination treatment of an alpha blocker with anticholinergic medication in BPH/LUTS through a systematic review and meta-analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28052499/ecosystem-carbon-density-and-allocation-across-a-chronosequence-of-longleaf-pine-forests
#4
Lisa J Samuelson, Thomas A Stokes, John R Butnor, Kurt H Johnsen, Carlos A Gonzalez-Benecke, Timothy A Martin, Wendell P Cropper, Pete H Anderson, Michael R Ramirez, John C Lewis
Forests can partially offset greenhouse gas emissions and contribute to climate change mitigation, mainly through increases in live biomass. We quantified carbon (C) density in 20 managed longleaf pine (Pinus palustris Mill.) forests ranging in age from 5 to 118 years located across the southeastern United States and estimated above- and belowground C trajectories. Ecosystem C stock (all pools including soil C) and aboveground live tree C increased nonlinearly with stand age and the modeled asymptotic maxima were 168 Mg C/ha and 80 Mg C/ha, respectively...
January 2017: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/28042051/l-carnitine-is-a-survival-factor-for-chilled-storage-of-rooster-semen-for-a-long-time
#5
A Fattah, M Sharafi, R Masoudi, A Shahverdi, V Esmaeili
Rooster sperm is sensitive to cooling, which restricts procedures to store sperms for extended periods of time for artificial insemination of commercial flocks. This study was conducted to evaluate the suitability of adding L-carnitine (LC) to chilled-storage of rooster sperm and its effects on sperm quality parameters and its fertility potential during storage at 5 °C. Pooled semen from roosters were divided into six equal aliquots and diluted with media supplemented with different concentrations of LC (0, 0...
December 29, 2016: Cryobiology
https://www.readbyqxmd.com/read/28041820/combined-alpha-delta-platelet-storage-pool-deficiency-is-associated-with-mutations-in-gfi1b
#6
Carlos R Ferreira, Dong Chen, Shirley M Abraham, David R Adams, Karen L Simon, May C Malicdan, Thomas C Markello, Meral Gunay-Aygun, William A Gahl
Combined alpha-delta platelet storage pool deficiency is characterized by the absence or reduction in the number of both alpha granules and dense bodies. This disorder can have variable severity as well as a variable inheritance pattern. We describe two patients from unrelated families with combined alpha-delta storage pool deficiency due to mutations in GFI1B, a zinc finger protein known to act as a transcriptional repressor of various genes. We demonstrate that this disease is associated with either a heterozygous mutation (de novo or familial) abrogating the binding of the zinc fingers with the promoter of its target genes, or by hypomorphic biallelic mutations in GFI1B leading to autosomal recessive inheritance...
December 18, 2016: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28031527/tumor-initiating-cells-of-breast-and-prostate-origin-show-alterations-in-the-expression-of-genes-related-to-iron-metabolism
#7
Zuzana Rychtarcikova, Sandra Lettlova, Veronika Tomkova, Vlasta Korenkova, Lucie Langerova, Ekaterina Simonova, Polina Zjablovskaja, Meritxell Alberich-Jorda, Jiri Neuzil, Jaroslav Truksa
The importance of iron in the growth and progression of tumors has been widely documented. In this report, we show that tumor-initiating cells (TICs), represented by spheres derived from the MCF7 cell line, exhibit higher intracellular labile iron pool, mitochondrial iron accumulation and are more susceptible to iron chelation. TICs also show activation of the IRP/IRE system, leading to higher iron uptake and decrease in iron storage, suggesting that level of properly assembled cytosolic iron-sulfur clusters (FeS) is reduced...
December 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/28019031/pathogen-inactivation-by-riboflavin-and-ultraviolet-light-illumination-accelerates-the-red-blood-cell-storage-lesion-and-promotes-eryptosis
#8
Syed M Qadri, Deborah Chen, Peter Schubert, Darian L Perruzza, Varsha Bhakta, Dana V Devine, William P Sheffield
BACKGROUND: Pathogen reduction treatment using riboflavin and ultraviolet light illumination (Mirasol) effectively reduces the risk of transfusion-transmitted infections. This treatment is currently licensed for only platelets and plasma products, while its application to whole blood (WB) to generate pathogen-inactivated red blood cells (RBCs) is under development. RBC storage lesion, constituting numerous morphologic and biochemical changes, influences RBC quality and limits shelf life...
December 26, 2016: Transfusion
https://www.readbyqxmd.com/read/28001307/storage-of-red-blood-cells-in-a-novel-polyolefin-blood-container-a-pilot-in-vitro-study
#9
H Gulliksson, S Meinke, A Ravizza, L Larsson, P Höglund
BACKGROUND AND OBJECTIVES: The present general plasticizer di-2-ethylhexyl-phthalate in polyvinylchloride (PVC) blood bags is only physically dispersed in PVC and will therefore leach into blood components. The objective of this study was to perform a first preliminary red blood cell (RBC) storage evaluation in a new blood bag manufactured of polyolefin without any inclusion of potentially migrating substances. STUDY DESIGN AND METHODS: This is a RBC storage study for 42 days...
January 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28001293/experiences-with-semi-routine-production-of-riboflavin-and-uv-b-pathogen-inactivated-platelet-concentrates-in-three-blood-centres
#10
P F van der Meer, C Couture, T Hervig, G Kruit, D V Devine, D de Korte, J-L Kerkhoffs
BACKGROUND: For a clinical platelet (PLT) transfusion trial conducted in three countries, the production of PLT concentrates (PCs) that were pathogen inactivated with the Mirasol technology was set up and validated. While the Mirasol procedure is applied to an established PLT product, the PLT processing procedure still had to be modified to ensure a treated PC was of sufficient quality. Further, the effect of simulated transport conditions and the effect of ambient light on Mirasol-treated PCs was determined...
January 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/27994525/changing-the-30-min-rule-in-canada-the-effect-of-room-temperature-on-bacterial-growth-in-red-blood-cells
#11
Sandra Ramirez-Arcos, Yuntong Kou, Éric Ducas, Louis Thibault
BACKGROUND: To maintain product quality and safety, the '30-min rule' requires the discard of red blood cells (RBCs) that are exposed to uncontrolled temperatures for more than 30 min. Recent studies suggest this rule may safely be extended to a 60-min rule. METHODS: A pool-and-split design study (N = 4) was run in parallel at Canadian Blood Services (SAGM RBCs) and Héma-Québec (AS-3 RBCs). RBCs were spiked with ∼1 colony-forming unit/ml of mesophilic and psychrophilic bacteria...
November 2016: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/27984662/long-term-structural-and-biomass-dynamics-of-virgin-tsuga-canadensis-pinus-strobus-forests-after-hurricane-disturbance
#12
Anthony W D'Amato, David A Orwig, David R Foster, Audrey Barker Plotkin, Peter K Schoonmaker, Maggie R Wagner
The development of old-growth forests in northeastern North America has largely been within the context of gap-scale disturbances given the rarity of stand-replacing disturbances. Using the 10-ha old-growth Harvard Tract and its associated 90-year history of measurements, including detailed surveys in 1989 and 2009, we document the long-term structural and biomass development of an old-growth Tsuga canadensis-Pinus strobus forest in southern New Hampshire, USA following a stand-replacing hurricane in 1938. Measurements of aboveground biomass pools were integrated with data from second- and old-growth T...
December 16, 2016: Ecology
https://www.readbyqxmd.com/read/27980503/stability-of-targeted-metabolite-profiles-of-urine-samples-under-different-storage-conditions
#13
Markus Rotter, Stefan Brandmaier, Cornelia Prehn, Jonathan Adam, Sylvia Rabstein, Katarzyna Gawrych, Thomas Brüning, Thomas Illig, Heiko Lickert, Jerzy Adamski, Rui Wang-Sattler
INTRODUCTION: Few studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS). OBJECTIVES: We investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics. METHODS: Pooled, fasting urine samples were collected and stored at -80 °C (biobank standard), -20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h...
2017: Metabolomics: Official Journal of the Metabolomic Society
https://www.readbyqxmd.com/read/27975331/historical-and-seasonal-dynamics-of-phosphorus-mobility-in-sancha-lake-of-southwest-china-s-sichuan-province
#14
Binyang Jia, Ya Tang, Bo Yang, Jen-How Huang
Phosphorus (P) fractionations in the surface sediment of Sancha Lake in China's southwestern Sichuan Province were examined to assess the potential P release at the water-sediment interface and to understand its seasonal (2009-2010) and historical dynamics (1989-2010) in the surface water. Elevated P concentrations were detected in the sediment at main reservoir inflow, south canal of the Dujiangyan irrigation network, and intensive cage fish farming area, accounting for 32 and 40% of current total P discharges...
January 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27939197/experimental-considerations-in-metal-mobilization-from-soil-by-chelating-ligands-the-influence-of-soil-solution-ratio-and-pre-equilibration-a-case-study-on-fe-acquisition-by-phytosiderophores
#15
W D C Schenkeveld, R L Kimber, M Walter, E Oburger, M Puschenreiter, S M Kraemer
The efficiency of chelating ligands in mobilizing metals from soils and sediments is generally examined under conditions remote from those under which they are exuded or applied in the field. This may lead to incorrect estimations of the mobilizing efficiency. The aim of this study was to establish the influence of the soil solution ratio (SSR) and pre-equilibration with electrolyte solution on metal mobilization and metal displacement. For this purpose a series of interaction experiments with a calcareous clay soil and a biogenic chelating agent, the phytosiderophore 2'-deoxymugineic acid (DMA) were carried out...
February 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/27939176/lack-of-impact-of-umbilical-cord-blood-unit-processing-techniques-on-clinical-outcomes-in-adult-double-cord-blood-transplant-recipients
#16
Sarah Nikiforow, Shuli Li, Karen Snow, Deborah Liney, Grace Shih-Hui Kao, Richard Haspel, Elizabeth J Shpall, Brett Glotzbecker, R Alejandro Sica, Philippe Armand, John Koreth, Vincent T Ho, Edwin P Alyea, Jerome Ritz, Robert J Soiffer, Joseph H Antin, Bimal Dey, Steven McAfee, Yi-Bin Chen, Thomas Spitzer, David Avigan, Corey S Cutler, Karen Ballen
BACKGROUND AIMS: Despite widespread use of umbilical cord blood (UCB) transplantation and distinct practice preferences displayed by individual UCB banks and transplant centers, little information exists on how processing variations affect patient outcomes. METHODS: We reviewed 133 adult double UCB transplants performed at a single center: 98 after reduced-intensity and 35 after myeloablative conditioning. Processing associated with contributing UCB banks and units was surveyed to identify differences in practice...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/27936548/enhanced-giant-piezoresistance-performance-of-sandwiched-zns-si-sio2-radial-heterostructure-nanotubes-for-nonvolatile-stress-memory-with-repeatable-writing-and-erasing
#17
Baochang Cheng, Li Xiong, Qiangsheng Cai, Haiping Shi, Jie Zhao, Xiaohui Su, Yanhe Xiao, Shuijin Lei
It is a challenge to realize nonvolatile stress-writing memory. Herein, we propose a strategy to construct rewritable stress information storage devices, consisting of deliberately designing individual sandwiched ZnS/Si/SiO2 radial heterostructure nanotubes synthesized by one-step thermal evaporation method. A bulk trap-related Poole-Frenkel hopping mechanism is proposed. Carriers are localized in a narrow bandgap Si intermediate layer; moreover, incorporated impurities and heterointerface defects can serve as charge trap centers or storage mediators...
December 21, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27927845/embryo-donation-and-understanding-of-kinship-the-impact-of-law-and-policy
#18
Jenni Millbank, Anita Stuhmcke, Isabel Karpin
STUDY QUESTION: What is the impact of law and policy upon the experience of embryo donation for reproductive use? SUMMARY ANSWER: Access to, and experience of, embryo donation are influenced by a number of external factors including laws that impose embryo storage limits, those that frame counselling and approval requirements and allow for, or mandate, donor identity disclosure. WHAT IS KNOWN ALREADY: To date only three qualitative studies in Australia and New Zealand have been completed on the experience of embryo donation for reproductive purposes, each with a small cohort of interviewees and divergent findings...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27919600/preanalytic-of-total-antioxidant-capacity-assays-performed-in-serum-plasma-urine-and-saliva
#19
REVIEW
Cristiano Ialongo
The investigation of oxidative stress (OS), its mechanisms and connections with human diseases, is a topic of interest with more than 36,000 PubMed citations to date. The OS can be approached either from the perspective of pro-oxidation, either of anti-oxidation, and both can be investigated considering individual chemical constituents or their pooled effect. Actually, as it is for any laboratory assay, whatever source of variability introduces a bias potentially undermining the test results regardless of its application...
December 3, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27892539/chemotypic-variation-in-terpenes-emitted-from-storage-pools-influences-early-aphid-colonisation-on-tansy
#20
Mary V Clancy, Sharon E Zytynska, Matthias Senft, Wolfgang W Weisser, Jörg-Peter Schnitzler
Tansy plants (Tanacetum vulgare L.) exhibit high chemical variation, particularly in mono- and sesquiterpenes that are stored in specialised glands on the plant surface. In the present work we investigated the effects of terpene chemotypes on Metopeurum fuscoviride, an aphid species specialised on tansy, and their tending ants, at the field scale. Previous studies have chemotyped tansy by assessing dominant compounds; here we propose a method of chemotyping using all volatile compounds that are likely emitted from the storage glands...
November 28, 2016: Scientific Reports
keyword
keyword
109249
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"