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https://www.readbyqxmd.com/read/28641229/effects-of-short-term-invasion-of-spartina-alterniflora-and-the-subsequent-restoration-of-native-mangroves-on-the-soil-organic-carbon-nitrogen-and-phosphorus-stock
#1
Jianxiang Feng, Jian Zhou, Liming Wang, Xiaowei Cui, Cunxin Ning, Hao Wu, Xiaoshan Zhu, Guanghui Lin
The exotic cordgrass Spartina alterniflora has severely invaded the mangrove wetlands in southern China and ecological restoration using native mangroves was conducted in an attempt to control this invasive species. In this study, the contents and pools of soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) were quantified to investigate the invasive effects of S. alterniflora and then to evaluate whether the ecological restoration of native mangrove could reverse those effects. S. alterniflora only showed significantly higher organic carbon content in the surface 0-10 cm of soil than in the uninvaded mudflat...
June 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28634352/differences-in-sequences-between-hbv-relaxed-circular-dna-and-covalently-closed-circular-dna
#2
Magda Rybicka, Anna Woziwodzka, Tomasz Romanowski, Piotr Stalke, Marcin Dręczewski, Krzysztof Piotr Bielawski
The hepatitis B virus (HBV) genome exists in two forms: circular covalently closed DNA (cccDNA) and relaxed circular DNA (RCDNA). Here, we investigated the presence of differences in the sequences of both forms in paired samples of serum and liver tissue. The serum and liver biopsy samples were collected at the same time from 67 chronically infected patients. The genotyping of the RCDNA and cccDNA was performed using mass spectrometry analysis. The HBV mutations located in the HBV pol (P) and the HBV basal core promoter/pre-core (BCP/PC) regions were included...
June 21, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28624109/the-use-of-old-donors-in-liver-transplantation
#3
REVIEW
Bobby V M Dasari, Andrea Schlegel, Hynek Mergental, M Thamara P R Perera
The process of ageing has an impact on the entire human body including the organ systems. In transplantation, professionals are daily faced with risk assessment of suitable donor offers , whether to accept a liver graft for a specific recipient. In this context, livers from elderly donors are more frequently accepted for transplantation, to increase the donor pool and compensate the high waiting list mortality. In the current practice it is not unusual to accept 60-year old donor livers for transplantation, as the donor demographics have significantly changed over the years...
April 2017: Best Practice & Research. Clinical Gastroenterology
https://www.readbyqxmd.com/read/28619669/sex-pheromone-in-the-moth-heliothis-virescens-is-produced-as-a-mixture-of-two-pools-de-novo-and-via-precursor-storage-in-glycerolipids
#4
Stephen P Foster, Karin G Anderson, Jérôme Casas
Most species of moths use a female-produced volatile sex pheromone, typically produced via de novo fatty acid synthesis in a specialized gland, for communication among mates. While de novo biosynthesis of pheromone (DNP) is rapid, suggesting transient precursor acids, substantial amounts of pheromone precursor (and other) acids are stored, predominantly in triacylglycerols in the pheromone gland. Whether these stored acids are converted to pheromone later or not has been the subject of some debate. Using a tracer/tracee approach, in which we fed female Heliothis virescens U-(13)C-glucose, we were able to distinguish two pools of pheromone, in which precursors were temporally separated (after and before feeding on labeled glucose): DNP synthesized from a mixed tracer/tracee acetyl CoA pool after feeding, and pheromone made from precursor acids primarily synthesized before feeding, which we call recycled precursor fat pheromone (RPP)...
June 12, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28618360/the-distribution-of-phosphorus-and-its-transformations-during-batch-growth-of-synechocystis
#5
Yun Zhou, Binh T Nguyen, Chen Zhou, Levi Straka, YenJung Sean Lai, Siqing Xia, Bruce E Rittmann
Phosphorus (P) is an essential nutrient that affects the growth and metabolism of microalgal biomass. Despite the obvious importance of P, the dynamics of how it is taken up and distributed in microalgae are largely undefined. In this study, we tracked the fate of P during batch growth of the cyanobacterium Synechocystis sp. PCC 6803. We determined the distribution of P in intracellular polymeric substances (IPS), extracellular polymeric substances (EPS), and soluble microbial products (SMP) for three initial ortho-phosphate concentrations...
June 9, 2017: Water Research
https://www.readbyqxmd.com/read/28618110/intravesical-vanilloids-for-treating-neurogenic-lower-urinary-tract-dysfunction-in-patients-with-multiple-sclerosis-a-systematic-review-and-meta-analysis-a-report-from-the-neuro-urology-promotion-committee-of-the-international-continence-society-ics
#6
REVIEW
Véronique Phé, Marc P Schneider, Benoit Peyronnet, Nadim Abo Youssef, Livio Mordasini, Emmanuel Chartier-Kastler, Lucas M Bachmann, Thomas M Kessler
AIMS: To systematically assess all available evidence on efficacy and safety of vanilloids for treating neurogenic lower urinary tract dysfunction (NLUTD) in patients with multiple sclerosis (MS). METHODS: This systematic review and meta-analysis was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement. Studies were identified by electronic search of Cochrane register, Embase, Medline, Scopus, (last search January 8, 2016)...
June 15, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28592692/genome-wide-temporal-expression-profiling-in-c-elegans-identifies-a-core-gene-set-related-to-long-term-memory
#7
Virginie Freytag, Sabine Probst, Nils Hadziselimovic, Csaba Boglari, Yannick Hauser, Fabian Peter, Bank Gabor Fenyves, Annette Milnik, Philippe Demougin, Vanja Vukojevic, Dominique J-F de Quervain, Andreas Papassotiropoulos, Attila Stetak
The identification of genes related to encoding, storage, and retrieval of memories is a major interest in neuroscience. In the current study, we analyzed the temporal gene expression changes in a neuronal mRNA pool during an olfactory long-term associative memory (LTAM) in C. elegans hermaphrodites. Here we identified a core set of 712 (538 upregulated and 174 downregulated) genes that follow three distinct temporal peaks demonstrating multiple gene regulation waves in LTAM. Comparing with the previously published positive LTAM gene sets (Lakhina et al...
June 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28588652/efficacy-and-safety-of-daily-mirabegron-50-mg-in-male-patients-with-overactive-bladder-a-critical-analysis-of-five-phase-iii-studies
#8
Andrea Tubaro, José E Batista, Victor W Nitti, Sender Herschorn, Christopher R Chapple, Mary Beth Blauwet, Emad Siddiqui, Moses Huang, Matthias Oelke
BACKGROUND: Oral pharmacotherapies to treat overactive bladder (OAB) are used less in men despite a similar prevalence of storage symptoms as women. The efficacy and safety of once-daily mirabegron 50 mg was evaluated in male OAB patients from five phase III studies that included placebo or antimuscarinic (tolterodine ER 4 mg or solifenacin 5 mg) as a comparator. METHODS: Three pooled 12-week placebo-controlled studies (mirabegron 50 mg versus placebo) and one 12-week non-inferiority phase IIIb study (BEYOND; mirabegron 50 mg versus solifenacin 5 mg) were used for efficacy (daily micturition frequency, urgency and incontinence episodes) and safety analyses...
December 2017: Therapeutic Advances in Urology
https://www.readbyqxmd.com/read/28579142/stability-of-lyophilized-pooled-sera-as-quality-control-materials-for-tumor-marker-assays-in-external-quality-assessment
#9
Sunyoung Ahn, Jungyong Park, Young Ran Kim, Jeong-Ho Kim, Hyon-Suk Kim
BACKGROUND: External quality assessment (EQA) requires stable quality control (QC) materials. We evaluated the stability of QC materials made of lyophilized and liquid pooled sera for the tumor markers α-fetoprotein, carcinoembryonic antigen, carbohydrate antigen 125, and carbohydrate antigen 19-9. METHODS: Specimens of the 4 tumor markers were collected from the sera of patients and stored at -20°C. After sera collection and pooling, liquid or lyophilized samples were stored at -20°C, 5°C, or room temperature...
June 1, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28560826/training-alters-the-distribution-of-perilipin-proteins-in-muscle-following-acute-free-fatty-acid-exposure
#10
S O Shepherd, J A Strauss, Q Wang, J J Dube, B Goodpaster, D G Mashek, L S Chow
Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyceride (IMTG) storage, we investigated the hypothesis that differential protein content of PLINs and their distribution with LDs may be linked to the diverse lipid storage in muscle between trained and sedentary individuals. Trained (n = 11) and sedentary (n = 10) subjects, matched for age, sex and BMI, received either a 6 h lipid or glycerol infusion in the setting of a concurrent hyperinsulinaemic-euglycaemic clamp...
May 31, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28557562/mesenchymal-stromal-cells-as-anti-inflammatory-and-regenerative-mediators-for-donor-kidneys-during-normothermic-machine-perfusion
#11
Jesus Maria Sierra-Parraga, Marco Eijken, James Hunter, Cyril Moers, Henri Leuvenink, Bjarne Møller, Rutger Ploeg, Carla C Baan, Bente Jespersen, Martin J Hoogduijn
There is a great demand for transplant kidneys for the treatment of end-stage kidney disease patients. To expand the donor pool, organs from older and comorbid brain death donors, so-called expanded criteria donors (ECD), as well as donation after circulatory death (DCD) donors, are considered for transplantation. However, the quality of these organs may be inferior to standard donor organs. A major issue affecting graft function and survival is ischemia reperfusion injury (IRI), which particularly affects kidneys from deceased donors...
May 30, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28554065/high-throughput-metataxonomic-characterization-of-the-raw-milk-microbiota-identifies-changes-reflecting-lactation-stage-and-storage-conditions
#12
Conor J Doyle, David Gleeson, Paul W O'Toole, Paul D Cotter
Low temperature is used to control the growth of bacteria in milk, both pre- and post-pasteurization. As the duration of refrigerated storage extends, psychrotrophs dominate the milk microbiota, that can produce heat stable lipases which negatively impact the organoleptic qualities of milk. Here we examine the influence that refrigeration temperature (2°C, 4°C and 6°C) and storage duration (96h) have on the microbiota composition (16S profiling) of raw bulk tank milk (BTM). To reflect a proposed change to current farming practices, raw milk was blended after each milking (8 milkings) and stored for five consecutive days in each temperature-specific tank...
May 22, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28541039/label-free-discrimination-of-rhizobial-bacteroids-and-mutants-by-single-cell-raman-microspectroscopy
#13
Jiabao Xu, Isabel Webb, Philip Poole, Wei E Huang
Symbiotic rhizobia in legumes account for a large portion of nitrogen fixation in the biosphere. Nitrogen fixation is an energy-demanding process requiring tight control of metabolism and redox state. It is of great interest to understand the bacteroid differentiation process and the roles of energy storage molecules, such as glycogen and polyhydroxybutyrate (PHB), in maintaining the Rhizobium-legume symbioses. Traditional biochemical assays for checking phenotypic changes of mutants require a large volume of starting materials, which is difficult for unculturable, terminally differentiated bacteroids...
June 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28536035/seasonality-can-induce-coexistence-of-multiple-bet-hedging-strategies-in-dictyostelium-discoideum-via-storage-effect
#14
Ricardo Martínez-García, Corina E Tarnita
The social amoeba Dictyostelium discoideum has been recently suggested as an example of bet-hedging in microbes. In the presence of resources, amoebae reproduce as unicellular organisms. Resource depletion, however, leads to a starvation phase in which the population splits between aggregators, which form a fruiting body made of a stalk and resistant spores, and non-aggregators, which remain as vegetative cells. Spores are favored when starvation periods are long, but vegetative cells can exploit resources in environments where food replenishes quickly...
May 20, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28531956/sediment-microbes-mediate-the-impact-of-nutrient-loading-on-blue-carbon-sequestration-by-mixed-seagrass-meadows
#15
Songlin Liu, Zhijian Jiang, Jingping Zhang, Yunchao Wu, Xiaoping Huang, Peter I Macreadie
Recent studies have reported significant variability in sediment organic carbon (SOC) storage capacity among seagrass species, but the factors driving this variability are poorly understood, limiting our ability to make informed decisions about which seagrass types are optimal for carbon offsetting and why. Here we show that differences in SOC storage capacity among species within the same geomorphic environment can be explained (in part) by below-ground processes in response to nutrient load; specifically, differences in the activity of microbes harboured by morphologically-different seagrass species...
December 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28527145/an-appraisal-of-indonesia-s-immense-peat-carbon-stock-using-national-peatland-maps-uncertainties-and-potential-losses-from-conversion
#16
REVIEW
Matthew Warren, Kristell Hergoualc'h, J Boone Kauffman, Daniel Murdiyarso, Randall Kolka
BACKGROUND: A large proportion of the world's tropical peatlands occur in Indonesia where rapid conversion and associated losses of carbon, biodiversity and ecosystem services have brought peatland management to the forefront of Indonesia's climate mitigation efforts. We evaluated peat volume from two commonly referenced maps of peat distribution and depth published by Wetlands International (WI) and the Indonesian Ministry of Agriculture (MoA), and used regionally specific values of carbon density to calculate carbon stocks...
December 2017: Carbon Balance and Management
https://www.readbyqxmd.com/read/28520947/synergistic-effect-of-spice-extracts-and-modified-atmospheric-packaging-towards-non-thermal-preservation-of-chicken-meat-under-refrigerated-storage
#17
M Sivarajan, U Lalithapriya, Peter Mariajenita, B Aafrin Vajiha, K Harini, D Madhushalini, M Sukumar
This study investigates the integrated approach of spice extracts and modified atmospheric packaging (MAP) chicken meat preservation. Specifically, extracts from clove (CL), cinnamon (CI) individually and in combination (3% w/w) along with MAP (30% CO2/70% N2 and 10% O2/30% CO2/60% N2) were used to increase the shelf life of fresh chicken meat stored at 4°C. The parameters evaluated as shelf life indications are microbiological (total viable count, Pseudomonas spp., lactic acid bacteria (LAB), and Enterobacteriaceae), physicochemical (pH, Lipid oxidation, color changes) and Sensory attributes...
May 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28520711/inhalational-chlorine-injuries-at-public-aquatic-venues-california-2008-2015
#18
Jason A Wilken, Michele DiMaggio, Matthew Kaufmann, Kevin O'Connor, Svetlana Smorodinsky, Christina Armatas, Tracy Barreau, Richard Kreutzer, Lino Ancheta
In June 2015, personnel from California's Contra Costa Health Services Environmental Health and Hazardous Materials (hazmat) divisions were alerted to a possible chemical release at a swimming pool in an outdoor municipal water park. Approximately 50 bathers were in the pool when symptoms began; 34 (68%) experienced vomiting, coughing, or eye irritation. Among these persons, 17 (50%) were treated at the scene by Contra Costa's Emergency Medical Services (EMS) and released, and 17 (50%) were transported to local emergency departments; five patients also were evaluated later at an emergency department or by a primary medical provider...
May 19, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28504409/multifunctional-liposomes-for-image-guided-intratumoral-chemo-phototherapy
#19
Dyego Miranda, Kevin Carter, Dandan Luo, Shuai Shao, Jumin Geng, Changning Li, Upendra Chitgupi, Steven G Turowski, Nasi Li, G Ekin Atilla-Gokcumen, Joseph A Spernyak, Jonathan F Lovell
Intratumoral (IT) drug injections reduce systemic toxicity, but delivered volumes and distribution can be inconsistent. To improve IT delivery paradigms, porphyrin-phospholipid (PoP) liposomes are passively loaded with three hydrophilic cargos: sulforhodamine B, a fluorophore; gadolinium-gadopentetic acid, a magnetic resonance (MR) agent; and oxaliplatin, a colorectal cancer chemotherapeutic. Liposome composition is optimized so that cargo is retained in serum and storage, but is released in less than 1 min with exposure to near infrared light...
May 15, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28500814/fta-cards-facilitate-storage-shipment-and-detection-of-arboviruses-in-infected-aedes-aegypti-collected-in-adult-mosquito-traps
#20
Sonja Hall-Mendelin, Glen R Hewitson, Doris Genge, Peter J Burtonclay, Amanda J De Jong, Alyssa T Pyke, Andrew F van den Hurk
AbstractThe utility of applying infected Aedes aegypti to Flinders Technology Associates (FTA(®)) cards for storage, transport, and detection of dengue, Zika, and Barmah Forest viruses was assessed in laboratory-based experiments. The mosquitoes had been removed from Gravid Aedes Traps maintained under conditions of high temperature and humidity. RNA of all viruses could be detected in infected mosquitoes on FTA cards either individually or in pools with uninfected mosquitoes, and stored for up to 28 days...
May 2017: American Journal of Tropical Medicine and Hygiene
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