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Storage pool

A S Varela-Stokes, S H Park, J V Stokes, N A Gavron, S I Lee, G M Moraru, S C Ricke
Our objective of this study was to explore the bacterial microbiome in fresh or fresh-frozen adult Amblyomma maculatum (Gulf Coast ticks) using extracts enriched for microbial DNA. We collected 100 questing adult A. maculatum, surface disinfected them, and extracted DNA from individual ticks collected the same day or after storage at -80 °C. Because only extracts with microbial DNA concentrations above 2 ng/μL were considered suitable for individual analysis, we expected fewer samples to meet these requirements...
March 1, 2018: Ticks and Tick-borne Diseases
Simon R Law, Daria Chrobok, Marta Juvany, Nicolas Delhomme, Pernilla Lindén, Bastiaan Brouwer, Abdul Ahad, Thomas Moritz, Stefan Jansson, Per Gardestrom, Olivier Keech
In plants, an individually darkened leaf (IDL) initiates senescence much more rapidly than a leaf from a whole darkened plant (DP). Combining transcriptomic and metabolomic approaches in Arabidopsis thaliana, we present an overview of the metabolic strategies that are employed in response to different darkening treatments. Under DP conditions, the perception of carbon starvation drove a profound metabolic readjustment in which lipids, branched-chain amino acids, and potentially monosaccharides released from cell wall loosening became important substrates for maintaining minimal ATP production...
March 9, 2018: Plant Physiology
J Mason Earles, Thorsten Knipfer, Aude Tixier, Jessica Orozco, Clarissa Reyes, Maciej A Zwieniecki, Craig R Brodersen, Andrew J McElrone
Starch is the primary energy storage molecule used by most terrestrial plants to fuel respiration and growth during periods of limited to no photosynthesis, and its depletion can drive plant mortality. Destructive techniques at coarse spatial scales exist to quantify starch, but these techniques face methodological challenges that can lead to uncertainty about the lability of tissue-specific starch pools and their role in plant survival. Here, we demonstrate how X-ray microcomputed tomography (microCT) and a machine learning algorithm can be coupled to quantify plant starch content in vivo, repeatedly and nondestructively over time in grapevine stems (Vitis spp...
March 8, 2018: New Phytologist
Diego A Miranda, William C Krause, Amaury Cazenave-Gassiot, Miyuki Suzawa, Hazel Escusa, Juat Chin Foo, Diyala S Shihadih, Andreas Stahl, Mark Fitch, Edna Nyangau, Marc Hellerstein, Markus R Wenk, David L Silver, Holly A Ingraham
Excess lipid accumulation is an early signature of nonalcoholic fatty liver disease (NAFLD). Although liver receptor homolog 1 (LRH-1) (encoded by NR5A2) is suppressed in human NAFLD, evidence linking this phospholipid-bound nuclear receptor to hepatic lipid metabolism is lacking. Here, we report an essential role for LRH-1 in hepatic lipid storage and phospholipid composition based on an acute hepatic KO of LRH-1 in adult mice (LRH-1AAV8-Cre mice). Indeed, LRH-1-deficient hepatocytes exhibited large cytosolic lipid droplets and increased triglycerides (TGs)...
March 8, 2018: JCI Insight
Laura Falchi, Grazia Galleri, Gian Mario Dore, Maria Teresa Zedda, Salvatore Pau, Luisa Bogliolo, Federica Ariu, Alessandra Pinna, Stefano Nieddu, Plinio Innocenzi, Sergio Ledda
BACKGROUND: Cerium oxide nanoparticles (CeO2 NPs) are able to store and release oxygen, conferring them scavenger activity against oxidative stress. However, their effects in reproductive systems are not yet well understood. The aim of the study was to investigate the effects of exposure of refrigerated ram semen to CeO2 NPs for 96 h on the main structural and kinematic parameters of spermatozoa. METHODS: The ejaculates of 5 Sarda rams were collected, pooled and diluted in a soybean lecithin extender...
March 6, 2018: Reproductive Biology and Endocrinology: RB&E
Juan C Begara-Morales, Mounira Chaki, Raquel Valderrama, Beatriz Sánchez-Calvo, Capilla Mata-Pérez, María N Padilla, Francisco J Corpas, Juan B Barroso
Nitric oxide (NO) has emerged as an essential biological messenger in plant biology that usually transmits its bioactivity by post-translational modifications such as S-nitrosylation, the reversible addition of a NO group to a protein cysteine residue leading to S-nitrosothiols (SNOs). In last year's, SNOs have emerged as key signalling molecules mainly involved in plant response to stress. Chief among SNOs is S-nitrosoglutathione (GSNO), generated by S-nitrosylation of the key antioxidant glutathione (GSH)...
March 1, 2018: Journal of Experimental Botany
Lin Zhao, Xiaodong Wu, Zhiwei Wang, Yu Sheng, Hongbing Fang, Yonghua Zhao, Guojie Hu, Wangping Li, Qiangqiang Pang, Jianzong Shi, Bentian Mo, Qian Wang, Xirui Ruan, Xiaodong Li, Yongjian Ding
There are several publications related to the soil organic carbon (SOC) on the Qinghai-Tibetan Plateau (QTP). However, most of these reports were from different parts of the plateau with various sampling depth. Here, we present the results from a systematic sampling and analysis of 200 soil pits. Most of the pits were deeper than 2 m from an east-west transect across the plateau. The SOC and total nitrogen (TN) pools of the 148 × 104  km2 , the area of the permafrost zone, for the upper 2 m soils calculated from the vegetation map were estimated to be 17...
February 26, 2018: Scientific Reports
Lucas E Nave, Grant M Domke, Kathryn L Hofmeister, Umakant Mishra, Charles H Perry, Brian F Walters, Christopher W Swanston
Soils are Earth's largest terrestrial carbon (C) pool, and their responsiveness to land use and management make them appealing targets for strategies to enhance C sequestration. Numerous studies have identified practices that increase soil C, but their inferences are often based on limited data extrapolated over large areas. Here, we combine 15,000 observations from two national-level databases with remote sensing information to address the impacts of reforestation on the sequestration of C in topsoils (uppermost mineral soil horizons)...
February 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
Parvez M Lokhandwala, Adrian O'Neal, Eshan U Patel, Patricia A R Brunker, Eric A Gehrie, Gang Zheng, Thomas S Kickler, Paul M Ness, Aaron A R Tobian
BACKGROUND: AABB standards state that cryoprecipitate should be transfused within 4 to 6 hours after thawing. We evaluated coagulation factor levels and sterility of thawed pooled cryoprecipitate to assess whether shelf life can be safely extended. STUDY DESIGN AND METHODS: Donor cryoprecipitate pools (n = 20, 10 group A, 10 group O) were held at ambient temperature and sampled at 0, 4, 8, 24, 48, 72, 96, and 120 hours post-thawing for fibrinogen, Factor (F)VIII, and von Willebrand factor (vWF) levels...
February 25, 2018: Transfusion
Steven M Garcia, Stanley Tamaki, Solomon Lee, Alvin Wong, Anthony Jose, Joanna Dreux, Gayle Kouklis, Hani Sbitany, Rahul Seth, P Daniel Knott, Chase Heaton, William R Ryan, Esther A Kim, Scott L Hansen, William Y Hoffman, Jason H Pomerantz
Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human muscle stem cell research has advanced slowly. Here, we describe a robust and predictable HuSC purification process that is effective for each human skeletal muscle tested and the development of storage protocols and transplantation models in dystrophin-deficient and wild-type recipients...
February 15, 2018: Stem Cell Reports
Lisa P Jacobson, Bryan Lau, Diane Catellier, Corette B Parker
PURPOSE OF REVIEW: A highly complex collaborative study design that pools and extends existing studies, such as the Environmental influences on Child Health Outcomes (ECHO) Program, requires a Data Analysis Center (DAC) with resources and expertise to create a secure environment for housing and analyzing the shared data, harmonize and structure the shared data for different purposes, and apply appropriate and innovative designs and analytic methods. The DAC, in partnership with cohort investigators, must ensure that results from ECHO-wide cohort analyses are appropriately interpreted and reproducible...
February 21, 2018: Current Opinion in Pediatrics
Lerraughn M Morgan, Emily R Miller, Ashok B Raj, Susan C Coventry, Jennifer D Elster
Angiomatoid fibrous histiocytoma is a rare soft tissue tumor usually discovered in young individuals. This tumor is often mistaken for a hematoma and typically misdiagnosed. It is commonly found in the extremities and may be associated with a site of recent or previous trauma. Characteristic histology includes nodules of histiocytoid spindle cells with pseudoangiomatoid spaces, fibrous pseudocapsules, and lymphoid cuffing. We describe the case of an 8-year-old girl who presented after incision and drainage of a superficial thigh lesion and experienced subsequent chronic bleeding of her wound...
February 22, 2018: Pediatrics
Shoichi Kageyama, Kojiro Nakamura, Bibo Ke, Ronald W Busuttil, Jerzy W Kupiec-Weglinski
Liver ischemia-reperfusion injury (IRI) represents a risk factor for early graft dysfunction and an obstacle to expanding donor pool in orthotopic liver transplantation (OLT). We have reported on the crucial role of macrophage Notch1 signaling in mouse warm hepatic IRI model. However, its clinical relevance or therapeutic potential remain unknown. Here, we used Serelaxin (SER), to verify Notch1 induction and putative hepatoprotective function in IR-stressed OLT. C57BL/6 mouse livers subjected to extended (18hr) cold storage were transplanted to syngeneic recipients...
February 21, 2018: American Journal of Transplantation
Lee Organick, Siena Dumas Ang, Yuan-Jyue Chen, Randolph Lopez, Sergey Yekhanin, Konstantin Makarychev, Miklos Z Racz, Govinda Kamath, Parikshit Gopalan, Bichlien Nguyen, Christopher N Takahashi, Sharon Newman, Hsing-Yeh Parker, Cyrus Rashtchian, Kendall Stewart, Gagan Gupta, Robert Carlson, John Mulligan, Douglas Carmean, Georg Seelig, Luis Ceze, Karin Strauss
Synthetic DNA is durable and can encode digital data with high density, making it an attractive medium for data storage. However, recovering stored data on a large-scale currently requires all the DNA in a pool to be sequenced, even if only a subset of the information needs to be extracted. Here, we encode and store 35 distinct files (over 200 MB of data), in more than 13 million DNA oligonucleotides, and show that we can recover each file individually and with no errors, using a random access approach. We design and validate a large library of primers that enable individual recovery of all files stored within the DNA...
February 19, 2018: Nature Biotechnology
Pasinee Kanaprach, Nutkridta Pongsakul, Nopporn Apiwattanakul, Chatchai Muanprasat, Sarayut Supapannachart, Pracha Nuntnarumit, Somchai Chutipongtanate
BACKGROUND: Donor human milk is considered the next best nutrition following mother's own milk to prevent neonatal infection and necrotizing enterocolitis in preterm infants who are admitted at neonatal intensive care unit. However, donor milk biofunctionalities after preparative processes have rarely been documented. OBJECTIVE: To evaluate biofunctionalities preserved in donor milk after preparative processes by cell-based assays. MATERIALS AND METHODS: Ten pools of donor milk were produced from 40 independent specimens...
February 16, 2018: Breastfeeding Medicine: the Official Journal of the Academy of Breastfeeding Medicine
Marco Amoruso, Lorenzo Alberio, Monika Nagy
No abstract text is available yet for this article.
February 12, 2018: American Journal of Hematology
Frank J Lebeda, Jeffrey J Zalatoris, Julia B Scheerer
Introduction: This position paper summarizes the development and the present status of Department of Defense (DoD) and other government policies and guidances regarding cloud computing services. Due to the heterogeneous and growing biomedical big datasets, cloud computing services offer an opportunity to mitigate the associated storage and analysis requirements. Having on-demand network access to a shared pool of flexible computing resources creates a consolidated system that should reduce potential duplications of effort in military biomedical research...
February 7, 2018: Military Medicine
Angelica E Patterson, Rachel Arkebauer, Crystal Quallo, Mary A Heskel, Ximeng Li, Natalie Boelman, Kevin L Griffin
The forests of the northeastern US are globally, one of the fastest growing terrestrial carbon sinks due to historical declines in large-scale agriculture, timber harvesting and fire disturbance. However, shifting range distributions of tree species with warming air temperatures are altering forest community composition and carbon dynamics. Here, we focus on respiration, a physiological process that is strongly temperature and species dependent. We specifically examined the response of respiration (R; CO2 release) to temperature in 10 broadleaved and six conifer species, as well as the respiratory quotient (RQ; ratio of CO2 released to O2 consumed) of nine broadleaved species that co-occur in the Hudson Highlands Region of New York, USA...
February 7, 2018: Tree Physiology
Tai-Hua Hu, Shiu-Cheung Lung, Zi-Wei Ye, Mee-Len Chye
Oxylipins are crucial components in plant wound responses that are mobilised via the plant vasculature. Previous studies have shown that the overexpression of an Arabidopsis acyl-CoA-binding protein, AtACBP3, led to an accumulation of oxylipin-containing galactolipids, and AtACBP3pro::BETA-GLUCURONIDASE (GUS) was expressed in the phloem of transgenic Arabidopsis. To investigate the role of AtACBP3 in the phloem, reverse transcription-polymerase chain reaction and western blot analysis of phloem exudates from the acbp3 mutant and wild type revealed that the AtACBP3 protein, but not its mRNA, was detected in the phloem sap...
2018: Frontiers in Plant Science
Gaël Le Croizier, Camille Lacroix, Sébastien Artigaud, Stéphane Le Floch, Jean Raffray, Virginie Penicaud, Valérie Coquillé, Julien Autier, Marie-Laure Rouget, Nicolas Le Bayon, Raymond Laë, Luis Tito De Morais
Impacted marine environments lead to metal accumulation in edible marine fish, ultimately impairing human health. Nevertheless, metal accumulation is highly variable among marine fish species. In addition to ecological features, differences in bioaccumulation can be attributed to species-related physiological processes, which were investigated in two marine fish present in the Canary Current Large Marine Ecosystem (CCLME), where natural and anthropogenic metal exposure occurs. The European sea bass Dicentrarchus labrax and Senegalese sole Solea senegalensis were exposed for two months to two environmentally realistic dietary cadmium (Cd) doses before a depuration period...
February 5, 2018: Environmental Pollution
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