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https://www.readbyqxmd.com/read/28651407/-homology-analysis-and-historical-tracing-for-inter-continental-burkholderia-pseudomallei-strains-of-sequence-type-562
#1
X Zheng, L X Wang, H Wu, H Chen, X Zhu, J R He, L X Xia, W Li
Objective: To understand the homology of sequence type 562 (ST562) strains of Burkholderia pseudomallei which circulated in two separate continents (Asia and Australia) at different times. Methods:SpeⅠrestriction fragments and 4-locus multiple locus variable number tandem repeat analysis (MLVA-4) profiles were extracted from MSHR5858 (ST562 Australia strain) and 350105 (ST562 historical strain of Hainan) genomes respectively by in silico analysis and then compared with the PFGE and MLVA-4 results of five ST562 clinical isolates from Hainan to test their homology...
May 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28650802/translational-motion-tracking-of-leg-joints-for-enhanced-prediction-of-walking-tasks
#2
Roman Mark Stolyarov, Gary Burnett, Hugh Herr
OBJECTIVE: Prediction of walking tasks in powered leg prostheses is an important problem in the development of biomimetic prosthesis controllers. This article proposes a novel method to predict upcoming walking tasks by estimating the translational motion of leg joints using an inertial measurement unit. METHODS: We asked six subjects with unilateral transtibial amputations to traverse flat ground, ramps, and stairs using a powered prosthesis while inertial signals were remotely collected...
June 22, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28649856/the-efficacy-of-inactivated-escherichia-coli-autovaccines-against-the-e-coli-peritonitis-syndrome-in-layers
#3
W J M Landman, J H H van Eck
Autogenous Escherichia coli vaccines to prevent the E. coli peritonitis syndrome (EPS) in laying hens are often used in the field, although their effectiveness has not been demonstrated yet. Therefore, in this study, which consisted of two experiments, their efficacy was assessed. In the first experiment, the EPS inducing ability of three E. coli isolates originating from bone marrow of hens that died due to EPS and with different Pulsed-Field Gel Electrophoresis patterns, was examined by intravenous inoculation of the isolates in 17 week-old brown layers...
June 26, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28649359/species-traits-and-interaction-rules-shape-a-species-rich-seed-dispersal-interaction-network
#4
Esther Sebastián-González, Mathias M Pires, Camila I Donatti, Paulo R Guimarães, Rodolfo Dirzo
Species phenotypic traits affect the interaction patterns and the organization of seed-dispersal interaction networks. Understanding the relationship between species characteristics and network structure help us understand the assembly of natural communities and how communities function. Here, we examine how species traits may affect the rules leading to patterns of interaction among plants and fruit-eating vertebrates. We study a species-rich seed-dispersal system using a model selection approach to examine whether the rules underlying network structure are driven by constraints in fruit resource exploitation, by preferential consumption of fruits by the frugivores, or by a combination of both...
June 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28649324/conceptualising-the-interactive-effects-of-climate-change-and-biological-invasions-on-subarctic-freshwater-fish
#5
REVIEW
Robert J Rolls, Brian Hayden, Kimmo K Kahilainen
Climate change and species invasions represent key threats to global biodiversity. Subarctic freshwaters are sentinels for understanding both stressors because the effects of climate change are disproportionately strong at high latitudes and invasion of temperate species is prevalent. Here, we summarize the environmental effects of climate change and illustrate the ecological responses of freshwater fishes to these effects, spanning individual, population, community and ecosystem levels. Climate change is modifying hydrological cycles across atmospheric, terrestrial and aquatic components of subarctic ecosystems, causing increases in ambient water temperature and nutrient availability...
June 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28649318/zooplankton-communities-and-bythotrephes-longimanus-in-lakes-of-the-montane-region-of-the-northern-alps
#6
Zsófia Horváth, Csaba F Vad, Christian Preiler, Julia Birtel, Blake Matthews, Radka Ptáčníková, Robert Ptacnik
Lakes in the Alps represent a considerable fraction of nutrient-poor lakes in Central Europe, with unique biodiversity and ecosystem properties. Although some individual lakes are well-studied, less knowledge is available on large-scale patterns essential to generalise the understanding of their functioning. Here, we aimed to describe crustacean zooplankton communities (Cladocera, Copepoda) and identify their environmental drivers in the pelagic zone of 54 oligotrophic lakes in the montane region of the Alps (400-1200 m) in Austria, Germany, and Switzerland, covering a spatial scale of 650 km...
May 2, 2017: Inland Waters
https://www.readbyqxmd.com/read/28649148/origins-and-genetic-diversity-of-introduced-populations-of-the-puerto-rican-red-eyed-coqu%C3%A3-eleutherodactylus-antillensis-in-saint-croix-u-s-virgin-islands-and-panam%C3%A3
#7
Brittany S Barker, Javier A Rodríguez-Robles
The Red-eyed Coquí, Eleutherodactylus antillensis, is a terrestrial frog endemic to the Puerto Rican Bank (Puerto Rico and numerous islands and cays off its eastern coast), in the eastern Caribbean Sea. The species was likely introduced in Saint Croix, an island c. 100 km southeast of Puerto Rico, in the late 1930s, and in Panamá City, Panamá, in the late 1950s or early 1960s, but the source(s) of these introductions are unknown. We analyzed sequence data from one mtDNA locus and four nuDNA introns to infer the origin(s) of the Saint Croix and Panamá City populations and quantify their genetic diversity...
July 2017: Copeia
https://www.readbyqxmd.com/read/28649031/noise-detection-on-ecg-based-on-agglomerative-clustering-of-morphological-features
#8
João Rodrigues, David Belo, Hugo Gamboa
Biosignals are usually contaminated with artifacts from limb movements, muscular contraction or electrical interference. Many algorithms of the literature, such as threshold methods and adaptive filters, focus on detecting these noisy patterns. This study introduces a novel method for noise and artifact detection in electrocardiogram based on time series clustering. The algorithm starts with the extraction of features that best characterize the shape and behaviour of the signal over time and groups its samples in separated clusters by means of an agglomerative clustering approach...
June 15, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28648083/temporal-signal-pattern-recognition-in-mass-spectrometry-a-method-for-rapid-identification-and-accurate-quantification-of-biomarkers-for-inborn-errors-of-metabolism-with-quality-assurance
#9
Alicia DiBattista, Nathan McIntosh, Monica Lamoureux, Osama Al-Dirbashi, Pranesh Chakraborty, Philip Britz-McKibbin
Mass spectrometry (MS)-based metabolomic initiatives that use conventional separation techniques are limited by low sample throughput and complicated data processing that contribute to false discoveries. Herein, we introduce a new strategy for unambiguous identification and accurate quantification of biomarkers for inborn errors of metabolism (IEM) from dried blood spots (DBS) with quality assurance. A multiplexed separation platform based on multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) was developed to provide comparable sample throughput to flow injection analysis-tandem MS (FIA-MS/MS), but with greater selectivity as required for confirmatory testing and discovery-based metabolite profiling of volume-restricted biospecimens...
June 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28647397/actinomyces-radicidentis-and-actinomyces-haliotis-coccoid-actinomyces-species-isolated-from-the-human-oral-cavity
#10
Rolf Claesson, Ulf Sjögren, Anders Esberg, Malin Brundin, Margareta Granlund
There are few reports on the bacterial species Actinomyces radicidentis in the literature. In this study, putative A. radicidentis isolates were collected from 16 root canal samples from 601 examined patients. The isolates were examined by biochemical tests, 16S rRNA gene sequencing, Arbitrarily-primed (AP-) PCR, antibiotic susceptibility testing, and MALDI-TOF analyses. In parallel, two A. radicidentis reference strains and two putative A. radicidentis isolates from United Kingdom were tested. Sixteen of the 18 isolates were confirmed as A...
June 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28647147/capillary-electrophoresis-with-stationary-nanogel-zones-of-galactosidase-and-erythrina-cristagalli-lectin-for-the-determination-of-%C3%AE-1-3-linked-galactose-in-glycans
#11
Lisa A Holland, Srikanth Gattu, Cassandra L Crihfield, Lloyd Bwanali
A thermally responsive nanogel is used to create stationary zones of enzyme and lectin in a separation capillary. Once patterned in the capillary, analyte is driven through the zone, where it is converted to a specific product if an enzyme is used or captured if a lectin is used. These stationary zones are easily expelled after the analysis and then re-patterned in the capillary. The nanogel is compatible with enzymes and lectins and improves the stability of galactosidase, enabling more cost-effective use of biological reagents that provide insight into glycan structure...
June 16, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28647145/a-new-peak-shape-analysis-approach-detection-and-quantitation-of-concurrent-fronting-tailing-and-their-effect-on-asymmetry-measurements
#12
M Farooq Wahab, Darshan C Patel, Daniel W Armstrong
Most peak shapes obtained in separation science depart from linearity for various reasons such as thermodynamic, kinetic, or flow based effects. An indication of the nature of asymmetry often helps in problem solving e.g. in column overloading, slurry packing, buffer mismatch, and extra-column band broadening. However, existing tests for symmetry/asymmetry only indicate the skewness in excess (tail or front) and not the presence of both. Two simple graphical approaches are presented to analyze peak shapes typically observed in gas, liquid, and supercritical fluid chromatography as well as capillary electrophoresis...
June 13, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28646716/histopathologic-differences-partially-distinguish-syndromic-aortic-diseases
#13
Kevin M Waters, Lisa M Rooper, Andrew Guajardo, Marc K Halushka
A variety of syndromic diseases such as Marfan syndrome, Loeys-Dietz syndrome, and bicuspid aortic valve with aneurysm along with risk factors of smoking and hypertension result in ascending aortic aneurysms and dissections. Historically, a complicated variety of terms have been used to describe a range of histopathologies that are present in resected specimens. As a result, no consistent patterns of histopathology have been reported. We used the recent Society for Cardiovascular Pathology/Association for European Cardiovascular Pathology consensus statement on nomenclature and diagnostic criteria for noninflammatory aortic disease to blindly evaluate 148 surgically resected specimens...
June 15, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/28646364/sinonasal-malignancies-incidence-and-histological-distribution-in-jamaica-1973-2007
#14
Tracey N Gibson, Dawn P McNaughton, Barrie Hanchard
PURPOSE: To determine the histological distribution and trends in incidence of sinonasal malignancies in Jamaica. METHODS: Cases of all sinonasal malignancies diagnosed between 1973 and 2007 were extracted from the archives of the Jamaica Cancer Registry. Data recorded for each case included age at diagnosis, sex, year of diagnosis, topography, and histology. Data were used to calculate frequencies, age-specific incidence rates, and age-standardized incidence rates (ASRs)...
June 23, 2017: Cancer Causes & Control: CCC
https://www.readbyqxmd.com/read/28646303/three-dimensional-fine-structure-of-the-organization-of-microtubules-in-neurite-varicosities-by-ultra-high-voltage-electron-microscope-tomography
#15
Tomoki Nishida, Ryoichi Yoshimura, Yasuhisa Endo
Neurite varicosities are highly specialized compartments that are involved in neurotransmitter/ neuromodulator release and provide a physiological platform for neural functions. However, it remains unclear how microtubule organization contributes to the form of varicosity. Here, we examine the three-dimensional structure of microtubules in varicosities of a differentiated PC12 neural cell line using ultra-high voltage electron microscope tomography. Three-dimensional imaging showed that a part of the varicosities contained an accumulation of organelles that were separated from parallel microtubule arrays...
June 23, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28645269/restricted-posture-in-dentistry-a-kinematic-analysis-of-orthodontists
#16
Daniela Ohlendorf, Christina Erbe, Imke Hauck, Jennifer Nowak, Ingo Hermanns, Dirk Ditchen, Rolf Ellegast, David A Groneberg
BACKGROUND: This study aims at identifying orthodontic activities with the highest frequency of unfavorable/awkward and static postures held over a period of more than 4 s based on kinematic analysis. Moreover, a separate analysis of static postures for orthodontic and non-orthodontic activities serves to evaluate the duration for which these particular postures are assumed. METHODS: In total, 21 (13f/8 m) orthodontists (age: 31.5 ± 3.8 years) participated in this study...
June 23, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28645100/interferential-effect-of-the-over-erupted-third-molar-on-chewing-movement
#17
Shaoxiong Guo, Baoyong Li, Kun Qi, Mian Zhang, Han Zhang, Donghui Guo, Xiaodong Liu, Meiqing Wang
OBJECTIVE: To clarify whether over-eruption of the mandibular third molar can disturb chewing movement. METHODS: Eighteen patients with a unilateral mandibular over-erupted third molar confirmed by both study cast observation and T-scan occlusal detection were selected from a sample of patients with complaints of temporomandibular disorder (TMD) symptoms. A unilateral gum-chewing trace was recorded separately for left and right side chewing by an electrognathography system...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28645069/controlled-single-bubble-cavitation-collapse-results-in-jet-induced-injury-in-brain-tissue
#18
Saranya Canchi, Karen Kelly, Yu Hong, Michael A King, Ghatu Subhash, Malisa Sarntinoranont
Multiscale damage due to cavitation is considered as a potential mechanism of traumatic brain injury (TBI) associated with explosion. In this study, we employed a TBI relevant hippocampal ex vivo slice model to induce bubble cavitation. Placement of single reproducible seed bubbles allowed control of size, number, and tissue location to visualize and measure deformation parameters. Maximum strain value was measured at 45 µs after bubble collapse, presented with a distinct contour and coincided temporally and spatially with the liquid jet...
June 15, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28642461/the-ca19-9-and-sialyl-tra-antigens-define-separate-subpopulations-of-pancreatic-cancer-cells
#19
Daniel Barnett, Ying Liu, Katie Partyka, Ying Huang, Huiyuan Tang, Galen Hostetter, Randall E Brand, Aatur D Singhi, Richard R Drake, Brian B Haab
Molecular markers to detect subtypes of cancer cells could facilitate more effective treatment. We recently identified a carbohydrate antigen, named sTRA, that is as accurate a serological biomarker of pancreatic cancer as the cancer antigen CA19-9. We hypothesized that the cancer cells producing sTRA are a different subpopulation than those producing CA19-9. The sTRA glycan was significantly elevated in tumor tissue relative to adjacent pancreatic tissue in 3 separate tissue microarrays covering 38 patients...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28641956/antibacterial-efficacy-of-nisin-bacteriophage-p100-and-sodium-lactate-against-listeria-monocytogenes-in-ready-to-eat-sliced-pork-ham
#20
Ana Cláudia L Figueiredo, Rogeria C C Almeida
The effectiveness of bacteriophage P100, nisin and sodium lactate, individually and in combination, in inhibiting Listeria monocytogenes in ready-to-eat pork ham slices was assessed. The antimicrobials were applied to the surfaces of ready-to-eat pork ham slices, which were inoculated with a mixture of L. monocytogenes. Among the individual antimicrobial treatments, bacteriophage P100 was the most effective, decreasing L. monocytogenes to undetectable levels at zero and 72h post-infection. Sodium lactate was the least effective treatment...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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