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multienzyme system

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https://www.readbyqxmd.com/read/29662082/hadd-a-novel-fatty-acid-synthase-type-ii-protein-is-essential-for-alpha-and-epoxy-mycolic-acid-biosynthesis-and-mycobacterial-fitness
#1
Cyril Lefebvre, Richard Boulon, Manuelle Ducoux, Sabine Gavalda, Françoise Laval, Stevie Jamet, Nathalie Eynard, Anne Lemassu, Kaymeuang Cam, Marie-Pierre Bousquet, Fabienne Bardou, Odile Burlet-Schiltz, Mamadou Daffé, Annaïk Quémard
Mycolic acids (MAs) have a strategic location within the mycobacterial envelope, deeply influencing its architecture and permeability, and play a determinant role in the pathogenicity of mycobacteria. The fatty acid synthase type II (FAS-II) multienzyme system is involved in their biosynthesis. A combination of pull-downs and proteomics analyses led to the discovery of a mycobacterial protein, HadD, displaying highly specific interactions with the dehydratase HadAB of FAS-II. In vitro activity assays and homology modeling showed that HadD is, like HadAB, a hot dog folded (R)-specific hydratase/dehydratase...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29603822/manganese-based-nanozymes-multienzyme-redox-activity-and-effect-on-the-nitric-oxide-produced-by-endothelial-nitric-oxide-synthase
#2
Namrata Singh, Geethika Motika, Sandeep M Eswarappa, Govindasamy Mugesh
Nanomaterials having enzyme-like activity (nanozymes) makes them suitable candidates for various biomedical applications. In this study, we demonstrate the morphology-dependent enzyme mimetic activity of Mn3O4 nanoparticles. It is found that Mn3O4 nanoparticles mimic the functions of all three cellular antioxidant enzymes- superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Interestingly, the nanozyme activity of Mn3O4 depends on various factors including size, morphology, surface area, redox property of the metal ions...
March 30, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29572992/therapeutic-nanoreactors-as-in-vivo-nanoplatforms-for-cancer-therapy
#3
Jean Felix Mukerabigwi, Zhishen Ge, Kazunori Kataoka
Therapeutic nanoreactors have been proposed as nanoplatforms to treat diseases through in situ production of therapeutic agents. When this treatment strategy is applied in cancer therapy, it can efficiently in situ produce the highly toxic anticancer drugs from low-toxic prodrugs or some biomolecules in tumor tissues, which can maximize the therapeutic efficacy with a significantly low systemic toxicity. An ideal therapeutic nanoreactor can provide the reaction space, protect the loaded fragile catalysts, target to the desired pathological site, and be selectively activated...
March 23, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29477859/bioamination-of-alkane-with-ammonium-by-an-artificially-designed-multienzyme-cascade
#4
Hui-Lei Yu, Tuo Li, Fei-Fei Chen, Xiao-Jing Luo, Aitao Li, Chao Yang, Gao-Wei Zheng, Jian-He Xu
Biocatalytic C-H amination is one of the most challenging tasks. C-H amination reaction can hardly be driven efficiently by solely one enzyme so far. Thus, enzymatic synergy represents an alternative strategy. Herein, we report an "Artificially Bioamination Pathway" for C-H amination of cyclohexane as a model substrate. Three enzymes, a monooxygenase P450BM3 mutant, an alcohol dehydrogenase ScCR from Streptomyces coelicolor and an amine dehydrogenase EsLeuDH from Exiguobacterium sibiricum, constituted a clean cascade reaction system with easy product isolation...
February 22, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29457453/exquisite-enzyme-fenton-biomimetic-catalysts-for-hydroxyl-radical-production-by-mimicking-an-enzyme-cascade
#5
Qi Zhang, Shuo Chen, Hua Wang, Hongtao Yu
Hydrogen peroxide (H2 O2 ) is a key reactant in the Fenton process. As a byproduct of enzymatic reaction, H2 O2 can be obtained via catalytical oxidation of glucose using glucose oxidase in the presence of O2 . Another oxidation product (gluconic acid) can suitably adjust the microenvironmental pH contributing to the Fe3+ /Fe2+ cycle in the Fenton reaction. Enzymes are extremely efficient at catalyzing a variety of reactions with high catalytic activity, substrate specificity, and yields in living organisms...
March 14, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29426820/a-mathematical-model-for-enzyme-clustering-in-glucose-metabolism
#6
Miji Jeon, Hye-Won Kang, Songon An
We have recently demonstrated that the rate-limiting enzymes in human glucose metabolism organize into cytoplasmic clusters to form a multienzyme complex, the glucosome, in at least three different sizes. Quantitative high-content imaging data support a hypothesis that the glucosome clusters regulate the direction of glucose flux between energy metabolism and building block biosynthesis in a cluster size-dependent manner. However, direct measurement of their functional contributions to cellular metabolism at subcellular levels has remained challenging...
February 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29408176/microwave-assisted-enzymatic-hydrolysis-of-dna-for-mass-spectrometric-analysis-a-new-strategy-for-accelerated-hydrolysis
#7
Sujatha Chilakala, Lan Li, Ye Feng, Yan Xu
Study of DNA base composition, DNA adducts and modification in its primary structure is a subject of interest in different fields of scientific research. Various methods like immunochemistry, capillary electrophoretic separation, chromatographic separation coupled with mass spectrometric (LC-MS/MS) detection have been developed for DNA analysis. During the past decade LC-MS/MS has emerged as a more sensitive and selective technique and now frequently used for the analysis of DNA. The workflow for the DNA analysis include DNA extraction, hydrolysis of DNA into mononucleosides and analysis...
April 1, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29223890/gold-core-ceria-shell-based-redox-active-nanozyme-mimicking-the-biological-multienzyme-complex-phenomenon
#8
Stuti Bhagat, N V Srikanth Vallabani, Vaithiyalingam Shutthanandan, Mark Bowden, Ajay S Karakoti, Sanjay Singh
Catalytically active individual gold (Au) and cerium oxide (CeO2 ) nanoparticles (NPs) are well known to exhibit specific enzyme-like activities, such as natural catalase, oxidase, superoxide dismutase, and peroxidase enzymes. These activities have been maneuvered to design several biological applications such as immunoassays, glucose detection, radiation and free radical protection and tissue engineering. In biological systems, multienzyme complexes are involved in catalyzing important reactions of essential metabolic processes such as respiration, biomolecule synthesis, and photosynthesis...
March 1, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29209155/optimization-of-quantitative-proteomic-analysis-of-clots-generated-from-plasma-of-patients-with-venous-thromboembolism
#9
Aneta Stachowicz, Jakub Siudut, Maciej Suski, Rafał Olszanecki, Ryszard Korbut, Anetta Undas, Jacek R Wiśniewski
Background: It is well known that fibrin network binds a large variety of proteins, including inhibitors and activators of fibrinolysis, which may affect clot properties, such as stability and susceptibility to fibrinolysis. Specific plasma clot composition differs between individuals and may change in disease states. However, the plasma clot proteome has not yet been in-depth analyzed, mainly due to technical difficulty related to the presence of a highly abundant protein-fibrinogen and fibrin that forms a plasma clot...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/29115133/pursuing-the-promise-of-enzymatic-enhancement-with-nanoparticle-assemblies
#10
James Nicholas Vranish, Mario G Ancona, Scott A Walper, Igor L Medintz
The growing emphasis on green chemistry, renewable resources, synthetic biology, regio-/stereospecific chemical transformations, and nanotechnology for providing new biological products and therapeutics is reinvigorating research into enzymatic catalysis. Although the promise is profound, many complex issues remain to be addressed before this effort will have a significant impact. Prime among these is to combat the degradation of enzymes frequently seen in ex vivo formats following immobilization to stabilize the enzymes for long-term application and to find ways of enhancing their activity...
March 6, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29050588/sequential-one-pot-multienzyme-synthesis-of-hyaluronan-and-its-derivative
#11
Shuang Li, Shuaishuai Wang, Xuan Fu, Xian-Wei Liu, Peng George Wang, Junqiang Fang
Hyaluronan (HA) is a linear polysaccharide composed of repeating disaccharide units. It has been well documented to play an array of biological functions in cancer events. Here, we reported a sequential one-pot multienzyme (OPME) strategy for in vitro synthesis of HA and its derivatives. The strategy, which combined in situ sugar nucleotides generation with HA chain polymerization, could convert cheap monosaccharides into HA polymers without consuming exogenous sugar nucleotide donors. HA polymers (number-average molecular weight ranged from 1...
December 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29022695/high-activity-and-convenient-ratio-control-dna-directed-coimmobilization-of-multiple-enzymes-on-multifunctionalized-magnetic-nanoparticles
#12
Ye Yang, Ruiqi Zhang, Bingnan Zhou, Jiayi Song, Ping Su, Yi Yang
The development of new methods for fabricating artificial multienzyme systems has attracted much interest because of the potential applications and the urgent need for multienzyme catalysts. Controlling the enzyme ratio is critical for improving the cooperative enzymatic activity in multienzyme systems. Herein, we introduce a versatile strategy for fabricating a multienzyme system by coimmobilizing horseradish peroxidase (HRP) and glucose oxidase (GOx) on magnetic nanoparticles multifunctionalized with dopamine derivatives through DNA-directed immobilization...
October 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28936496/h-pylori-%C3%AE-1-3-4-fucosyltransferase-hp3-4ft-catalyzed-one-pot-multienzyme-opme-synthesis-of-lewis-antigens-and-human-milk-fucosides
#13
Hai Yu, Yanhong Li, Zhigang Wu, Lei Li, Jie Zeng, Chao Zhao, Yijing Wu, Nova Tasnima, Jing Wang, Huaide Liu, Madhusudhan Reddy Gadi, Wanyi Guan, Peng G Wang, Xi Chen
Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component in lacto-N-neotetraose (LNnT)...
October 5, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28921955/characterization-of-site-specific-glycosylation-in-influenza-a-virus-hemagglutinin-produced-by-spodoptera-frugiperda-insect-cell-line
#14
Yan-Jun Liu, Shiaw-Lin Wu, Kerry R Love, William S Hancock
Influenza hemagglutinin is a surface glycoprotein related to virus invasion and host immune system response. Understanding site specific glycosylation of hemagglutinin will increase our knowledge about virus evolution and can improve the design and quality of vaccines. In our study, we used glycoproteomic analysis based on multienzyme digestion followed by LC tandem MS analysis to determine the glycosylation of Influenza hemagglutinin (H1/A/California/04/2009) using the following steps: PNGaseF treatment combined with trypsin or pepsin digestion was used to determine the glycosites and glycan occupancy...
October 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28866761/t-protein-is-present-in-large-excess-over-the-other-proteins-of-the-glycine-cleavage-system-in-leaves-of-arabidopsis
#15
Stefan Timm, Jonas Giese, Nadja Engel, Maria Wittmiß, Alexandra Florian, Alisdair R Fernie, Hermann Bauwe
T-protein is present in large excess over the other proteins of the glycine cleavage system in leaves of Arabidopsis and therefore, exerts little control over the photorespiratory pathway. T-protein is the aminomethyltransferase of the glycine cleavage multienzyme system (GCS), also known as the glycine decarboxylase complex, and essential for photorespiration and one-carbon metabolism. Here, we studied what effects varying levels of the GCS T-protein would have on GCS activity, the operation of the photorespiratory pathway, photosynthesis, and plant growth...
January 2018: Planta
https://www.readbyqxmd.com/read/28813159/enhancing-enzyme-activity-and-immobilization-in-nanostructured-inorganic-enzyme-complexes
#16
Xuye Lang, Lingling Zhu, Yingning Gao, Ian Wheeldon
Understanding the chemical and physical interactions at the interface of protein surfaces and inorganic crystals has important implications in the advancement of immobilized enzyme catalysis. Recently, enzyme-inorganic hybrid complexes have been demonstrated as effective materials for enzyme immobilization. The precipitation of phosphate nanocrystals in the presence of enzymes creates enzyme-Cu3(PO4)2·3H2O particles with high surface-to-volume ratios, enhanced activity, and increased stability. Here, we begin to develop a mechanistic understanding of enzyme loading in such complexes...
August 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28801642/extreme-anoxia-tolerance-in-crucian-carp-and-goldfish-through-neofunctionalization-of-duplicated-genes-creating-a-new-ethanol-producing-pyruvate-decarboxylase-pathway
#17
Cathrine E Fagernes, Kåre-Olav Stensløkken, Åsmund K Røhr, Michael Berenbrink, Stian Ellefsen, Göran E Nilsson
Without oxygen, most vertebrates die within minutes as they cannot meet cellular energy demands with anaerobic metabolism. However, fish of the genus Carassius (crucian carp and goldfish) have evolved a specialized metabolic system that allows them to survive prolonged periods without oxygen by producing ethanol as their metabolic end-product. Here we show that this has been made possible by the evolution of a pyruvate decarboxylase, analogous to that in brewer's yeast and the first described in vertebrates, in addition to a specialized alcohol dehydrogenase...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694846/synthetic-co2-fixation-enzyme-cascades-immobilized-on-self-assembled-nanostructures-that-enhance-co2-o2-selectivity-of-rubisco
#18
Sriram Satagopan, Yuan Sun, Jon R Parquette, F Robert Tabita
BACKGROUND: With increasing concerns over global warming and depletion of fossil-fuel reserves, it is attractive to develop innovative strategies to assimilate CO2, a greenhouse gas, into usable organic carbon. Cell-free systems can be designed to operate as catalytic platforms with enzymes that offer exceptional selectivity and efficiency, without the need to support ancillary reactions of metabolic pathways operating in intact cells. Such systems are yet to be exploited for applications involving CO2 utilization and subsequent conversion to valuable products, including biofuels...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28520394/in-vitro-metabolic-engineering-of-amorpha-4-11-diene-biosynthesis-at-enhanced-rate-and-specific-yield-of-production
#19
Xixian Chen, Congqiang Zhang, Ruiyang Zou, Gregory Stephanopoulos, Heng-Phon Too
In vitro metabolic engineering is an alternative approach to cell-based biosynthesis. It offers unprecedented flexibility for the study of biochemical pathways, thus providing useful information for the rational design and assembly of reaction modules. Herein, we took the advantage of in vitro metabolic engineering to initially gain insight into the regulatory network of a reconstituted amorpha-4,11-diene (AD) synthetic pathway. Guided by lin-log approximation, we rapidly identified the hitherto unrecognized inhibition of adenosine triphosphate (ATP) on both farnesyl pyrophosphate synthase (FPPS) and amorpha-4,11-diene synthase (ADS)...
September 15, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28429326/wax-ester-fermentation-and-its-application-for-biofuel-production
#20
REVIEW
Hiroshi Inui, Takahiro Ishikawa, Masahiro Tamoi
In Euglena cells under anaerobic conditions, paramylon, the storage polysaccharide, is promptly degraded and converted to wax esters. The wax esters synthesized are composed of saturated fatty acids and alcohols with chain lengths of 10-18, and the major constituents are myristic acid and myristyl alcohol. Since the anaerobic cells gain ATP through the conversion of paramylon to wax esters, the phenomenon is named "wax ester fermentation". The wax ester fermentation is quite unique in that the end products, i...
2017: Advances in Experimental Medicine and Biology
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