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https://www.readbyqxmd.com/read/29353162/monolayers-of-a-tetrazine-containing-gemini-amphiphile-interplays-with-biomembrane-lipids
#1
Hiromichi Nakahara, Masayori Hagimori, Takahiro Mukai, Osamu Shibata
The property of a newly synthesized tetrazine derivative comprised of double C18-saturated hydrocarbon chain (C18-rTz-C18) has been studied in situ at the air-water interface. C18-rTz-C18 or a gemini amphiphile contributes to restriction of its tetrazine moiety on the interface, which is expected to be used for bioimaging and analytical reagents. Herein, to understand lateral interactions between Tz and biomembrane constituents, we investigated the interfacial behavior of Langmuir monolayers composed of C18-rTz-C18 and biomembrane lipids such as DPPC, DPPG, DPPE, PSM, and Cholesterol (Ch)...
January 17, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29353004/leishmania-tropica-isolates-from-non-healed-and-healed-patients-in-iran-a-molecular-typing-and-phylogenetic-analysis
#2
Mehdi Bamorovat, Iraj Sharifi, Mohammad Ali Mohammadi, Sana Eybpoosh, Saeid Nasibi, Mohammad Reza Aflatoonian, Ahmad Khosravi
The precise identification of the parasite species causing leishmaniasis is essential for selecting proper treatment modality. The present study aims to compare the nucleotide variations of the ITS1, 7SL RNA, and Hsp70 sequences between non-healed and healed anthroponotic cutaneous leishmaniasis (ACL) patients in major foci in Iran. A case-control study was carried out from September 2015 to October 2016 in the cities of Kerman and Bam, in the southeast of Iran. Randomly selected skin-scraping lesions of 40 patients (20 non-healed and 20 healed) were examined and the organisms were grown in a culture medium...
January 15, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29351213/cyclin-b3-deficiency-impairs-germline-stem-cell-maintenance-and-its-overexpression-delays-cystoblast-differentiation-in-drosophila-ovary
#3
Dongsheng Chen, Lijuan Zhou, Fuling Sun, Mingzhong Sun, Xiaoqian Tao
It is well known that cyclinB3 (cycB3) plays a key role in the control of cell cycle progression. However, whether cycB3 is involved in stem cell fate determination remains unknown. The Drosophila ovary provides an exclusive model for studying the intrinsic and extrinsic factors that modulate the fate of germline stem cells (GSCs). Here, using this model, we show that DrosophilacycB3 plays a new role in controlling the fate of germline stem cells (GSC). Results from cycB3 genetic analyses demonstrate that cycB3 is intrinsically required for GSC maintenance...
January 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29327677/autologous-smooth-muscle-progenitor-cells-enhance-regeneration-of-tissue-engineered-bladder
#4
Liuhua Zhou, Jiadong Xia, Pengji Wang, Ruipeng Jia, Jun Hua Zheng, Xudong Yao, Yun Chen, Yutian Dai, Bin Yang
Tissue engineering techniques provide a great potential to de novo construct a histological bladder. Smooth muscle regeneration is extremely important for the functional recovery of engineered neo-bladder. However, many challenges remain for the use of bladder smooth muscle cells (SMCs) as the cell sources. Recent evidences showed that smooth muscle progenitor cells (SPCs) in the peripheral blood have the capacity of differentiating into SMCs, while their use for bladder regeneration hasn't yet been reported...
January 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29316036/inhibition-of-ire1-results-in-decreased-scar-formation
#5
Tatiana V Boyko, Rakesh Bam, Dadi Jiang, Zhen Wang, Namrata Bhatia, Misha C Tran, Michael T Longaker, Albert C Koong, George P Yang
Wound healing is characterized by the production of large amounts of protein necessary to replace lost cellular mass and extracellular matrix. The unfolded protein response (UPR) is an important adaptive cellular response to increased protein synthesis. One of the main components of the UPR is IRE1, an ER transmembrane protein with endonuclease activity that produces the activated form of the transcription factor XBP1. Using luciferase reporter mice for Xbp1 splicing, we showed that IRE1 was up-regulated during excisional wound healing at the time in wound healing consistent with that of the proliferative phase, when the majority of protein synthesis for cellular proliferation and matrix deposition occurs...
January 8, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29313189/supportive-properties-of-basement-membrane-layer-of-human-amniotic-membrane-enable-development-of-tissue-engineering-applications
#6
Sonia Iranpour, Nasser Mahdavi-Shahri, Raheleh Miri, Halimeh Hasanzadeh, Hamid Reza Bidkhori, Hojjat Naderi-Meshkin, Ehsan Zahabi, Maryam M Matin
Human amniotic membrane (HAM) has been widely used as a natural scaffold in tissue engineering due to many of its unique biological properties such as providing growth factors, cytokines and tissue inhibitors of metalloproteinases. This study aimed at finding the most suitable and supportive layer of HAM as a delivery system for autologous or allogeneic cell transplantation. Three different layers of HAM were examined including basement membrane, epithelial and stromal layers. In order to prepare the basement membrane, de-epithelialization was performed using 0...
January 8, 2018: Cell and Tissue Banking
https://www.readbyqxmd.com/read/29311257/the-bam-complex-catalyzes-efficient-insertion-of-bacterial-outer-membrane-proteins-into-membrane-vesicles-of-variable-lipid-composition
#7
Sunyia Hussain, Harris D Bernstein
Most proteins that reside in the bacterial outer membrane (OM) have a distinctive "β-barrel" architecture, but the assembly of these proteins is poorly understood. The spontaneous assembly of OM proteins (OMPs) into pure lipid vesicles has been studied extensively, but often requires non-physiological conditions and time scales and is strongly influenced by properties of the lipid bilayer including surface charge, thickness, and fluidity. Furthermore, the membrane insertion of OMPs in vivo is catalyzed by a heterooligomer called the β-barrel assembly machinery (Bam) complex...
January 8, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29302576/investigating-the-prevalence-of-preterm-birth-in-iranian-population-a-systematic-review-and-meta-analysis
#8
Nasibeh Sharifi, Somayyeh Khazaeian, Reza Pakzad, Azita Fathnezhad Kazemi, Hashemmieh Chehreh
Introduction: Despite medical advances, preterm delivery remains a global problem in developed and developing countries. The present study was aimed at conducting a systematic review and meta-analysis of studies on the prevalence of preterm delivery in Iran. Methods: This study was carried out on studies conducted in Iran by searching databases of SID, Magiran, Irandoc, MEDLIB, Iranmedex, PubMed, Web of science, Google Scholar and Scopus. The search was conducted using advanced search and keywords of preterm delivery and equivalents of it in Mesh and their Farsi's Synonymous in all articles from 2000-2016...
December 2017: Journal of Caring Sciences
https://www.readbyqxmd.com/read/29282013/an-investigation-of-factors-predicting-the-type-of-bladder-antimuscarinics-initiated-in-medicare-nursing-homes-residents
#9
Daniela C Moga, Qishan Wu, Pratik Doshi, Amie J Goodin
BACKGROUND: To examine factors predicting type of bladder antimuscarinics (BAM) initiated in nursing home (NH) residents. METHODS: Incident BAM initiators following NH admission were identified by constructing a retrospective cohort from Medicare files and Minimum Data Set (MDS). Participants included all residents 65 years and older admitted in Medicare-certified NH between January 1, 2007 and December 31, 2008 who were prescribed BAM and had continuous Medicare (Part A, B, and D) enrollment...
December 28, 2017: BMC Geriatrics
https://www.readbyqxmd.com/read/29274499/impact-of-leakage-delay-on-bifurcation-in-high-order-fractional-bam-neural-networks
#10
Chengdai Huang, Jinde Cao
The effects of leakage delay on the dynamics of neural networks with integer-order have lately been received considerable attention. It has been confirmed that fractional neural networks more appropriately uncover the dynamical properties of neural networks, but the results of fractional neural networks with leakage delay are relatively few. This paper primarily concentrates on the issue of bifurcation for high-order fractional bidirectional associative memory(BAM) neural networks involving leakage delay. The first attempt is made to tackle the stability and bifurcation of high-order fractional BAM neural networks with time delay in leakage terms in this paper...
December 6, 2017: Neural Networks: the Official Journal of the International Neural Network Society
https://www.readbyqxmd.com/read/29241545/the-germline-linker-histone-dbigh1-and-the-translational-regulator-bam-form-a-repressor-loop-essential-for-male-germ-stem-cell-differentiation
#11
Albert Carbonell, Salvador Pérez-Montero, Paula Climent-Cantó, Oscar Reina, Fernando Azorín
Drosophila spermatogenesis constitutes a paradigmatic system to study maintenance, proliferation, and differentiation of adult stem cell lineages. Each Drosophila testis contains 6-12 germ stem cells (GSCs) that divide asymmetrically to produce gonialblast cells that undergo four transit-amplifying (TA) spermatogonial divisions before entering spermatocyte differentiation. Mechanisms governing these crucial transitions are not fully understood. Here, we report the essential role of the germline linker histone dBigH1 during early spermatogenesis...
December 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/29229778/structural-and-functional-insights-into-the-role-of-bamd-and-bame-within-the-%C3%AE-barrel-assembly-machinery-in-neisseria-gonorrhoeae
#12
Aleksandra E Sikora, Igor H Wierzbicki, Ryszard A Zielke, Rachael F Ryner, Konstantin V Korotkov, Susan K Buchanan, Nicholas Noinaj
The β-barrel assembly machinery (BAM) is a conserved multi-component protein complex responsible for the biogenesis of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. Given its role in the production of OMPs for survival and pathogenesis, BAM represents an attractive target for the development of therapeutic interventions including drugs and vaccines against multi-drug resistant bacteria such as Neisseria gonorrhoeae The first structure of BamA, the central component of BAM, was from N...
December 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29227961/global-exponential-stability-and-lag-synchronization-for-delayed-memristive-fuzzy-cohen-grossberg-bam-neural-networks-with-impulses
#13
Wengui Yang, Wenwu Yu, Jinde Cao, Fuad E Alsaadi, Tasawar Hayat
This paper investigates the stability and lag synchronization for memristor-based fuzzy Cohen-Grossberg bidirectional associative memory (BAM) neural networks with mixed delays (asynchronous time delays and continuously distributed delays) and impulses. By applying the inequality analysis technique, homeomorphism theory and some suitable Lyapunov-Krasovskii functionals, some new sufficient conditions for the uniqueness and global exponential stability of equilibrium point are established. Furthermore, we obtain several sufficient criteria concerning globally exponential lag synchronization for the proposed system based on the framework of Filippov solution, differential inclusion theory and control theory...
November 23, 2017: Neural Networks: the Official Journal of the International Neural Network Society
https://www.readbyqxmd.com/read/29203775/glandular-morphometrics-for-objective-grading-of-colorectal-adenocarcinoma-histology-images
#14
Ruqayya Awan, Korsuk Sirinukunwattana, David Epstein, Samuel Jefferyes, Uvais Qidwai, Zia Aftab, Imaad Mujeeb, David Snead, Nasir Rajpoot
Determining the grade of colon cancer from tissue slides is a routine part of the pathological analysis. In the case of colorectal adenocarcinoma (CRA), grading is partly determined by morphology and degree of formation of glandular structures. Achieving consistency between pathologists is difficult due to the subjective nature of grading assessment. An objective grading using computer algorithms will be more consistent, and will be able to analyse images in more detail. In this paper, we measure the shape of glands with a novel metric that we call the Best Alignment Metric (BAM)...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29202265/kernel-bayesian-art-and-artmap
#15
Naoki Masuyama, Chu Kiong Loo, Farhan Dawood
Adaptive Resonance Theory (ART) is one of the successful approaches to resolving "the plasticity-stability dilemma" in neural networks, and its supervised learning model called ARTMAP is a powerful tool for classification. Among several improvements, such as Fuzzy or Gaussian based models, the state of art model is Bayesian based one, while solving the drawbacks of others. However, it is known that the Bayesian approach for the high dimensional and a large number of data requires high computational cost, and the covariance matrix in likelihood becomes unstable...
November 10, 2017: Neural Networks: the Official Journal of the International Neural Network Society
https://www.readbyqxmd.com/read/29198563/transient-transcriptional-silencing-alters-the-cell-cycle-to-promote-germline-stem-cell-differentiation-in-drosophila
#16
Pooja Flora, Sean Schowalter, SiuWah Wong-Deyrup, Matthew DeGennaro, Mohamad Ali Nasrallah, Prashanth Rangan
Transcriptional silencing is a conserved process used by embryonic germ cells to repress somatic fate and maintain totipotency and immortality. In Drosophila, this transcriptional silencing is mediated by polar granule component (pgc). Here, we show that in the adult ovary, pgc is required for timely germline stem cell (GSC) differentiation. Pgc is expressed transiently in the immediate GSC daughter (pre-cystoblast), where it mediates a pulse of transcriptional silencing. This transcriptional silencing mediated by pgc indirectly promotes the accumulation of Cyclin B (CycB) and cell cycle progression into late-G2 phase, when the differentiation factor bag of marbles (bam) is expressed...
November 30, 2017: Developmental Biology
https://www.readbyqxmd.com/read/29197585/studying-the-assembly-of-the-bam-complex-in-native-membranes-by-cellular-solid-state-nmr-spectroscopy
#17
Cecilia Pinto, Deni Mance, Manon Julien, Mark Daniels, Markus Weingarth, Marc Baldus
Significant progress has been made in obtaining a structural insight into the assembly of the β-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane mimetic and revealed structural variations in the central domain, BamA, as well as the lipoprotein BamC. We have used cellular solid-state NMR spectroscopy to examine the entire BamABCDE complex in native outer membranes and obtained data on the BamCDE subcomplex in outer membranes, in addition to synthetic bilayers...
November 29, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/29189770/langmuir-blodgett-nanotemplates-for-protein-crystallography
#18
Eugenia Pechkova, Claudio Nicolini
The new generation of synchrotrons and microfocused beamlines has enabled great progress in X-ray protein crystallography, resulting in new 3D atomic structures for proteins of high interest to the pharmaceutical industry and life sciences. It is, however, often still challenging to produce protein crystals of sufficient size and quality (order, intensity of diffraction, radiation stability). In this protocol, we provide instructions for performing the Langmuir-Blodgett (LB) nanotemplate method, a crystallization approach that can be used for any protein (including membrane proteins)...
December 2017: Nature Protocols
https://www.readbyqxmd.com/read/29186284/calq-compression-of-quality-values-of-aligned-sequencing-data
#19
Jan Voges, Jörn Ostermann, Mikel Hernaez
Motivation: Recent advancements in high-throughput sequencing technology have led to a rapid growth of genomic data. Several lossless compression schemes have been proposed for the coding of such data present in the form of raw FASTQ files and aligned SAM/BAM files. However, due to their high entropy, losslessly compressed quality values account for about 80% of the size of compressed files. For the quality values, we present a novel lossy compression scheme named CALQ. By controlling the coarseness of quality value quantization with a statistical genotyping model, we minimize the impact of the introduced distortion on downstream analyses...
November 23, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29184896/formative-evaluation-on-cultural-tailoring-breathing-awareness-meditation-smartphone-apps-to-reduce-stress-and-blood-pressure
#20
John C Sieverdes, Zachary W Adams, Lynne Nemeth, Brenda Brunner-Jackson, Martina Mueller, Ashley Anderson, Sachin Patel, Luke Sox, Frank A Treiber
Background: Chronic stress is an independent risk factor for essential hypertension (EH), cardiovascular disease (CVD), and is sometimes confronted by mal-adaptive coping behaviors (e.g., stress eating, excessive alcohol consumption, etc.). Pre-essential hypertension (preEH) is the leading predictor of future EH status. Breathing awareness meditation (BAM) can result in clinically beneficial blood pressure (BP) reductions, though face-to-face sessions presents barriers to reach those in need...
2017: MHealth
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