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Siavash Atashgahi, Bastian Hornung, Marcelle J van der Waals, Ulisses Nunes da Rocha, Floor Hugenholtz, Bart Nijsse, Douwe Molenaar, Rob van Spanning, Alfons J M Stams, Jan Gerritse, Hauke Smidt
In this study, we report transcription of genes involved in aerobic and anaerobic benzene degradation pathways in a benzene-degrading denitrifying continuous culture. Transcripts associated with the family Peptococcaceae dominated all samples (21-36% relative abundance) indicating their key role in the community. We found a highly transcribed gene cluster encoding a presumed anaerobic benzene carboxylase (AbcA and AbcD) and a benzoate-coenzyme A ligase (BzlA). Predicted gene products showed >96% amino acid identity and similar gene order to the corresponding benzene degradation gene cluster described previously, providing further evidence for anaerobic benzene activation via carboxylation...
March 14, 2018: Scientific Reports
Samuel Y S Wong, Yu Ying Sun, Aaroy T Y Chan, Maria K W Leung, David V K Chao, Carole C K Li, King K H Chan, Wai Kwong Tang, Trevor Mazzucchelli, Alma M L Au, Benjamin H K Yip
PURPOSE: We undertook a randomized controlled trial to assess the efficacy of group-based behavioral activation with mindfulness (BAM) for treating subthreshold depression in primary care in Hong Kong. METHODS: We recruited adult patients aged 18 years or older with subthreshold depression from public primary care clinics and randomly assigned them to a BAM intervention group or a usual care group. The BAM group was provided with eight 2-hour weekly BAM sessions by trained allied health care workers...
March 2018: Annals of Family Medicine
Cynthia Mohandas, Riccardo Bonadonna, Fariba Shojee-Moradie, Nicola Jackson, Linda Boselli, K George M M Alberti, Janet L Peacock, A Margot Umpleby, Stephanie A Amiel, Louise M Goff
AIMS: Populations of African ancestry suffer high rates of type 2 diabetes (T2D) compared to Caucasians. Phenotypic differences in pre-diabetic populations, particularly marked hyperinsulinaemia, suggest ethnic distinctions in T2D pathophysiology. We tested the hypothesis that men of Black (West) African (BAM) ethnicity with early T2D would have greater insulin secretory deficits compared to White Europeans (WEM), following the pre-diabetic hypersecretion. METHODS: In 19 BAM and 15 WEM, matched for age, BMI and duration diabetes, we assessed and modelled insulin secretory responses to hyperglycaemia stimulated intravenously (hyperglycaemic clamp) and orally (meal tolerance test)...
March 8, 2018: Diabetes, Obesity & Metabolism
Leyan Nian, Kui Wu, Guijie He, Zhihua Yang, Shilie Pan
In the exploration of new infrared nonlinear optical (IR NLO) materials, element substitution has been developed as an effective way to adjust the structural features and material performances. A series of new IR NLO materials have been discovered in the I-Ba-MIV -Q system ( I = Li, Na, Cu, and Ag; MIV = Si, Ge, and Sn; Q = S and Se), and they undergo interesting structural transformation with different element substitution except Li analogues. Herein, we have successfully synthesized three selenides with different space groups (Ag2 BaSiSe4 : I4̅2 m; Ag2 BaGeSe4 and Ag2 BaSnSe4 : I222) in the above system and studied their properties through experimental and theoretical methods...
March 6, 2018: Inorganic Chemistry
Mohan Penumala, Raveendra Babu Zinka, Jeelan Basha Shaik, Suresh Kumar Reddy Mallepalli, Ramakrishna Vadde, Damu Gangaiah Amooru
BACKGROUND: Extensive epidemiological and clinical studies revealed that Alzheimer's Disease (AD) and Type 2 Diabetes Mellitus (T2D) are most likely to appear simultaneously in aged people as T2D is a major risk factor for AD. Therefore, development of potential multifunctional agents for dual therapy of AD and T2D has received much attention. Buchanania axillaris, Hemidesmus indicus and Rhus mysorensis have been used extensively in popular medicine. The present study was aimed at phytochemical profiling and evaluating multifunctional ability of titled plants in the AD and T2D dual therapy...
March 2, 2018: BMC Complementary and Alternative Medicine
Ruijin Wang, Yi Guo, Hengjiang Liu, Yuning Chen, Yazhuo Shang, Honglai Liu
The effect of chitin nanoparticles on surface behavior of lipid systems containing dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) is studied by surface pressure (π)-area (A) isotherms, polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS), Brewster angle microscopy (BAM). The variation of surface behavior of DPPC/DPPG monolayers is induced mainly by electrostatic interactions between nanoparticles and head groups of phospholipids. At lower surface pressure, nanoparticles can penetrate into the monolayers and the positive charges carried by nanoparticles benefits the enrichment of phospholipid molecules at surface, which not only increases the mean molecular area but also hinders the formation of phospholipid liquid-condensed (LC) phase...
February 7, 2018: Journal of Colloid and Interface Science
Sergio Villalpando-Guzmán, Carlos Ramón Vázquez-Quiñones, Iván Natividad-Bonifacio, Everardo Curiel-Quesada, Elsa Irma Quiñones-Ramírez, Carlos Vázquez-Salinas
BACKGROUND: Food of animal origin is often involved in salmonellosis outbreaks. AIM: To evaluate the frequency of Salmonella enterica in chicken, beef and pork ground meat (a total of 2,592 samples) obtained from travelling markets and supermarkets at the Iztapalapa area of Mexico City, in order to determine the antimicrobial susceptibility and adherence capacity of isolated strains. METHODS: Isolation of S. enterica was carried out according to the BAM-FDA, the microbial susceptibility according with CLSI and adherence assay on HEp-2 cell line according with Baffone et al...
October 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Ellen Koepf, Manuela Richert, Bjoern Braunschweig, Rudolf Schroeder, Gerald Brezesinski, Wolfgang Friess
Both, formulation parameters and the presence of liquid-air interfaces are known to affect the aggregation of protein drugs. In this study, the impact of pH on the liquid-air interfacial behavior of three proteins, a polyclonal and two monoclonal antibodies (IgG, mAB1 and mAB2 ) was investigated using different surface sensitive methods. Equilibrium surface pressure values revealed only a minor impact of pH. Infrared Reflectance Absorbance Spectroscopy (IRRAS) proved not only the presence of the proteins at the interface but also showed that the secondary structure was not considerably affected by the adsorption to the interface independent of pH between pH 3 and 9...
February 24, 2018: International Journal of Pharmaceutics
Huihui Sun, Marina Resmini, Ali Zarbakhsh
Understanding the interaction of nanoparticles (NP) with ceramide lipids is important in developing strategies to overcome the formidable obstacle that is skin. This paper presents studies of interactions between N-isopropylacrylamide nanogels, crosslinked with 30% N,N'-methylenebisacrylamide, and model ceramide lipid monolayers at the air-water interface as a function of temperature. In the case of the mixed ceramide/cholesterol/behenic acid monolayer, the interaction of nanogels with the ceramide was strongly mediated by the fatty acids...
February 17, 2018: Journal of Colloid and Interface Science
Ahmad Ali Noorbala, Seyed Abbas Bagheri Yazdi, Soghrat Faghihzadeh, Koorosh Kamali, Elham Faghihzadeh, Ahmad Hajebi, Shahin Akhondzadeh, Parisa Divsalar, Nahid Kaviani, Zeinab Sarhadi, Ahdieh Bashar
INTRODUCTION: This research aims to determine the mental health status of population aged 15 and over in the province of Kerman in 2015. METHODS: The statistical population of this cross-sectional field survey consisted of residents of urban and rural areas of Kerman in Iran. An estimated sample size of 1200 people was chosen using systematic random cluster sampling. The access was provided by the contribution of Geographical Post Office of Kerman, Jiroft and Bam cities...
November 1, 2017: Archives of Iranian Medicine
Harsh Trivedi, Vladimir Shvartsman, Marco S A Medeiros, Rob Pullar, Doru Lupascu
Local observation of the stress mediated magnetoelectric (ME) effect in composites has gained a great deal of interest over the last decades. However, there is an apparent lack of rigorous methods for a quantitative characterization of the ME effect at the local scale, especially in polycrystalline microstructures. In the present work, we address this issue by locally probing surface magnetic state of barium titante - hexagonal barium ferrite (BaTiO<sub>3</sub> - BaFe<sub>12</sub>O<sub>19</sub>) ceramic composites using Magnetic Force Microscopy (MFM)...
February 22, 2018: Nanotechnology
James Lee, Holly A Sutterlin, Joseph S Wzorek, Michael D Mandler, Christine L Hagan, Marcin Grabowicz, David Tomasek, Mary D May, Elizabeth M Hart, Thomas J Silhavy, Daniel Kahne
The β-barrel assembly machine (Bam) complex folds and inserts integral membrane proteins into the outer membrane of Gram-negative bacteria. The two essential components of the complex, BamA and BamD, both interact with substrates, but how the two coordinate with each other during assembly is not clear. To elucidate aspects of this process we slowed the assembly of an essential β-barrel substrate of the Bam complex, LptD, by changing a conserved residue near the C terminus. This defective substrate is recruited to the Bam complex via BamD but is unable to integrate into the membrane efficiently...
February 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
Michał Flasiński, Sara Kowal, Marcin Broniatowski, Pawel Wydro
Langmuir monolayers were used to study the influence of four commercially applied parabens on multicomponent systems composed of lipid species characteristic for the cellular membrane of microorganisms found in carbohydrates and proteins reach products, including food and cosmetics. The aim of the undertaken studies was to shed new light onto the problem of parabens interactions with membrane lipids and their affinity toward monolayers differing as regards the composition, mutual lipid ratios and the physicochemical properties...
February 12, 2018: Journal of Physical Chemistry. B
Taemook Kim, Hogyu David Seo, Lothar Hennighausen, Daeyoup Lee, Keunsoo Kang
Octopus-toolkit is a stand-alone application for retrieving and processing large sets of next-generation sequencing (NGS) data with a single step. Octopus-toolkit is an automated set-up-and-analysis pipeline utilizing the Aspera, SRA Toolkit, FastQC, Trimmomatic, HISAT2, STAR, Samtools, and HOMER applications. All the applications are installed on the user's computer when the program starts. Upon the installation, it can automatically retrieve original files of various epigenomic and transcriptomic data sets, including ChIP-seq, ATAC-seq, DNase-seq, MeDIP-seq, MNase-seq and RNA-seq, from the gene expression omnibus data repository...
February 6, 2018: Nucleic Acids Research
Wouter S P Jong, Maaike Schillemans, Corinne M Ten Hagen-Jongman, Joen Luirink, Peter van Ulsen
Monomeric autotransporters have been extensively used for export of recombinant proteins to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such constructs is the passage of the outer membrane, which is facilitated by the β-domain at the C terminus of an autotransporter in conjunction with the Bam complex in the outer membrane. We have evaluated eight β-domain constructs for their capacity to secrete fused proteins to the cell surface. These constructs derive from the monomeric autotransporters Hbp, IgA protease, Ag43 and EstA and the trimeric autotransporter Hia, which all were selected because they have been previously used for secretion of recombinant proteins...
2018: PloS One
Benjamin T Mayne, Shalem Y Leemaqz, Sam Buckberry, Carlos M Rodriguez Lopez, Claire T Roberts, Tina Bianco-Miotto, James Breen
Genotyping-by-sequencing (GBS) or restriction-site associated DNA marker sequencing (RAD-seq) is a practical and cost-effective method for analysing large genomes from high diversity species. This method of sequencing, coupled with methylation-sensitive enzymes (often referred to as methylation-sensitive restriction enzyme sequencing or MRE-seq), is an effective tool to study DNA methylation in parts of the genome that are inaccessible in other sequencing techniques or are not annotated in microarray technologies...
February 1, 2018: Scientific Reports
Parasmani Pageni, Peng Yang, Yung Pin Chen, Yucheng Huang, Marpe Bam, Tianyu Zhu, Mitzi Nagarkatti, Brian C Benicewicz, Alan W Decho, Chuanbing Tang
Inappropriate and frequent use of antibiotics has led to the development of antibiotic-resistant bacteria, which cause infectious diseases that are difficult to treat. With the rising threat of antibiotic resistance, the need to develop effective new antimicrobial agents is prominent. We report antimicrobial metallopolymer nanoparticles, which were prepared by surface-initiated reversible addition-fragmentation chain transfer polymerization of a cobaltocenium-containing methacrylate monomer from silica nanoparticles...
January 31, 2018: Biomacromolecules
Homa Sadeghian, Rouzbeh Motiei-Langroudi
Oedema refers to the excessive accumulation of fluid within intercellular tissues as a result of disequilibrium between the capillary hydrostatic and oncotic pressure gradients. Lumbar disc herniation (LDH) commonly causes lower back pain and radicular leg pain. We report a 57-year-old female who presented to the neurosurgery clinic of the Bam University of Medical Sciences, Bam, Iran, in 2015 with pain and pitting oedema in the bilateral lower extremities. Magnetic resonance imaging confirmed a diagnosis of LDH of the L3-L4 and L4-L5 vertebrae...
November 2017: Sultan Qaboos University Medical Journal
Lijuan Feng, Zhen Shi, Jing Xie, Binbin Ma, Xin Chen
Tissue homeostasis depends on the ability of tissue-specific adult stem cells to maintain a balance between proliferation and differentiation, as well as ensure DNA damage repair. Here, we use the Drosophila male germline stem cell system to study how a chromatin factor, enhancer of polycomb [E(Pc)], regulates the proliferation-to-differentiation (mitosis-to-meiosis) transition and DNA damage repair. We identified two critical targets of E(Pc). First, E(Pc) represses CycB transcription, likely through modulating H4 acetylation...
January 23, 2018: Cell Death and Differentiation
Joseph P Salisbury, Neha U Keshav, Anthony D Sossong, Ned T Sahin
BACKGROUND: Lightweight and portable devices that objectively measure concussion-related impairments could improve injury detection and critical decision-making in contact sports and the military, where brain injuries commonly occur but remain underreported. Current standard assessments often rely heavily on subjective methods such as symptom self-reporting. Head-mounted wearables, such as smartglasses, provide an emerging platform for consideration that could deliver the range of assessments necessary to develop a rapid and objective screen for brain injury...
January 23, 2018: JMIR MHealth and UHealth
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