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https://www.readbyqxmd.com/read/30230973/tip60-complex-promotes-expression-of-a-differentiation-factor-to-regulate-germline-differentiation-in-female-drosophila
#1
Alicia McCarthy, Aron Deiulio, Elliot Todd Martin, Maitreyi Upadhyay, Prashanth Rangan
Germline stem cells (GSCs) self-renew and differentiate to sustain a continuous production of gametes. In the female Drosophila germ line, two differentiation factors, bag of marbles ( bam) and benign gonial cell neoplasm ( bgcn), work in concert in the stem cell daughter to promote the generation. In GSCs, bam transcription is repressed by signaling from the niche and is activated in stem cell daughters. In contrast, bgcn is transcribed in both the GSCs and stem cell daughters, but little is known about how bgcn is transcriptionally modulated...
September 19, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/30184862/ce-3-mn-2-activated-ca-7-po-4-2-sio-4-2-efficient-luminescent-materials-for-multifunctional-applications
#2
Jia Zhang, Lei Zhao, Xintian Bian, Guibin Chen
To develop new efficient phosphors for LEDs based on multifunctional applications, a series of Ce3+ /Mn2+ activated Ca7 (PO4 )2 (SiO4 )2 (CPS) samples were prepared by solid-state reaction method. Upon 365 nm excitation, a broad emission band around 439 nm in the Ce3+ -single-doped CPS was observed. The optimal Ce3+ concentration was determined to be 3%, for which the quantum efficiency was obtained to be 90.4%, higher than that of the commercial BAM phosphor. By monitoring 458 nm, an intense and broad excitation band was found from 240 to 400 nm, which can match well with the near-ultraviolet (NUV) LED chip...
September 3, 2018: Optics Express
https://www.readbyqxmd.com/read/30177884/engineering-of-human-skeletal-muscle-with-an-autologous-deposited-extracellular-matrix
#3
Lieven Thorrez, Katherine DiSano, Janet Shansky, Herman Vandenburgh
Adult skeletal muscle progenitor cells can be embedded in an extracellular matrix (ECM) and tissue-engineered to form bio-artificial muscles (BAMs), composed of aligned post-mitotic myofibers. The ECM proteins which have been used most commonly are collagen type I and fibrin. Fibrin allows for in vitro vasculogenesis, however, high concentrations of fibrinolysis inhibitors are needed to inhibit degradation of the ECM and subsequent loss of BAM tissue structure. For in vivo implantation, fibrinolysis inhibition may prove difficult or even harmful to the host...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/30154813/quaternary-structure-salt-sensitivity-and-allosteric-regulation-of-%C3%AE-amylase2-from-arabidopsis-thaliana
#4
Jonathan D Monroe, Lauren E Pope, Jillian S Breault, Christopher E Berndsen, Amanda R Storm
The β-amylase family in Arabidopsis thaliana has nine members, four of which are both plastid-localized and, based on active-site sequence conservation, potentially capable of hydrolyzing starch to maltose. We recently reported that one of these enzymes, BAM2, is catalytically active in the presence of physiological levels of KCl, exhibits sigmoidal kinetics with a Hill coefficient of over 3, is tetrameric, has a putative secondary binding site (SBS) for starch, and is highly co-expressed with other starch metabolizing enzymes...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/30150583/identification-and-characterization-of-lrr-rlk-family-genes-in-potato-reveal-their-involvement-in-peptide-signaling-of-cell-fate-decisions-and-biotic-abiotic-stress-responses
#5
Xiaoxu Li, Ahmad Salman, Cun Guo, Jing Yu, Songxiao Cao, Xiaoming Gao, Wei Li, Hong Li, Yongfeng Guo
Leucine-rich repeat receptor-like kinases (LRR-RLKs) represent the largest subfamily of receptor-like kinases (RLKs) and play important roles in regulating growth, development, and stress responses in plants. In this study, 246 LRR-RLK genes were identified in the potato ( Solanum tuberosum ) genome, which were further classified into 14 subfamilies. Gene structure analysis revealed that genes within the same subgroup shared similar exon/intron structures. A signature small peptide recognition motif (RxR) was found to be largely conserved within members of subfamily IX, suggesting that these members may recognize peptide signals as ligands...
August 27, 2018: Cells
https://www.readbyqxmd.com/read/30144189/1-amino-triazole-transition-metal-complexes-as-laser-ignitable-and-lead-free-primary-explosives
#6
Joerg Stierstorfer, Thomas M Klapötke, Norbert Szimhardt, Maximilian H H Wurzenberger, Lukas Zeisel, Michael S Gruhne, Marcus Lommel
This unique complex study describes two isomeric aminotriazoles as auspicious, nitrogen-rich ligands for energetic coordination compounds (ECC) in order to replace commonly used, highly poisonous and environmentally harmful lead-based primary explosives. The triazoles have been obtained via easily scalable and convenient synthesis routes solely requiring commercially available starting materials. 1-Amino-1,2,3-triazole (1, 1-ATRI) and for the first time 1-amino-1,2,4-triazole (2, 1A-1,2,4-TRI) could be implemented as ligands to form highly energetic transition metal(II) complexes...
August 24, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/30125090/sequence-specific-solution-nmr-assignments-of-the-%C3%AE-barrel-insertase-bama-to-monitor-its-conformational-ensemble-at-the-atomic-level
#7
Jean-Baptiste Hartmann, Michael Zahn, Irena Matečko Burmann, Stefan Bibow, Sebastian Hiller
β-barrel outer membrane proteins (Omps) are key functional components of the outer membranes of Gram-negative bacteria, mitochondria, and plastids. In bacteria, their biogenesis requires the β-barrel-assembly machinery (Bam) with the central insertase BamA, but the exact translocation and insertion mechanism remains elusive. The BamA insertase features a loosely closed gating region between the first and last β-strand 16. Here, we describe ∼70% complete sequence-specific NMR resonance assignments of the transmembrane region of the BamA β-barrel in detergent micelles...
September 12, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/30117244/highly-regioselective-domino-benzannulation-reaction-of-buta-1-3-diynes-to-construct-irregular-nanographenes
#8
Wenlong Yang, Radha Bam, Vincent J Catalano, Wesley A Chalifoux
The properties of nanographenes can be tuned by changing their shapes, therefore the development of new methods suitable for the synthesis of various nanographenes is highly desirable. Described herein is an intramolecular InCl3 /AgNTf2 -catalyzed regioselective domino benzannulation reaction of buta-1,3-diynes to build irregular nanographenes. Different nanographene compounds were easily obtained in moderate to high yields through careful design of the precursor compounds. This new domino reaction was successfully applied to a fourfold alkyne benzannulation of dimethoxy-1,1'-binaphthalene derivatives to arrive at novel chiral butterfly ligand precursors...
August 16, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/30116880/brief-report-length-of-ileal-resection-correlates-with-severity-of-bile-acid-malabsorption-in-crohn-s-disease
#9
Thomas Skouras, Susanna Dodd, Yash Prasad, Joseph Rassam, Nazreen Morley, Sreedhar Subramanian
PURPOSE: Bile acid malabsorption (BAM) is a common cause of diarrhoea in Crohn's disease (CD) patients with ileal resection. BAM is usually diagnosed by selenium-labelled homotaurocholic acid test (75SeHCAT) but its availability is limited. Consequently, a large proportion of patients either remain undiagnosed or subject to empirical therapy. There is a paucity of studies examining the correlation between length of ileal resection and severity of BAM, which will be of use to clinicians with no recourse to diagnostic testing for BAM...
August 16, 2018: International Journal of Colorectal Disease
https://www.readbyqxmd.com/read/30111779/human-tissue-engineered-skeletal-muscle-a-novel-3d-in-vitro-model-for-drug-disposition-and-toxicity-after-intramuscular-injection
#10
D Gholobova, M Gerard, L Decroix, L Desender, N Callewaert, P Annaert, L Thorrez
The development of laboratory-grown tissues, referred to as organoids, bio-artificial tissue or tissue-engineered constructs, is clearly expanding. We describe for the first time how engineered human muscles can be applied as a pre- or non-clinical model for intramuscular drug injection to further decrease and complement the use of in vivo animal studies. The human bio-artificial muscle (BAM) is formed in a seven day tissue engineering procedure during which human myoblasts fuse and differentiate to aligned myofibers in an extracellular matrix...
August 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30107065/identification-of-a-novel-post-insertion-step-in-the-assembly-of-a-bacterial-outer-membrane-protein
#11
Janine H Peterson, Sunyia Hussain, Harris D Bernstein
Although the barrel assembly machinery (Bam) complex has been shown to facilitate the insertion of β barrel proteins into the bacterial outer membrane (OM), the stage at which β barrels fold is unknown. Here we describe insights into β barrel assembly that emerged from an analysis of a member of the autotransporter family of OM proteins (EspP) in E. coli. EspP contains an extracellular "passenger" domain that is translocated across the OM and then released from the covalently linked β barrel domain in an intra-barrel cleavage reaction...
August 14, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/30087180/c-terminal-kink-formation-is-required-for-lateral-gating-in-bama
#12
Karl Lundquist, Jeremy Bakelar, Nicholas Noinaj, James C Gumbart
In Gram-negative bacteria, the outer membrane contains primarily β-barrel transmembrane proteins and lipoproteins. The insertion and assembly of β-barrel outer-membrane proteins (OMPs) is mediated by the β-barrel assembly machinery (BAM) complex, the core component of which is the 16-stranded transmembrane β-barrel BamA. Recent studies have indicated a possible role played by the seam between the first and last β-barrel strands of BamA in the OMP insertion process through lateral gating and a destabilized membrane region...
August 21, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/30085010/on-display-autotransporter-secretion-and-application
#13
Peter van Ulsen, Katinka M Zinner, Wouter S P Jong, Joen Luirink
The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins that pass the inner and outer membrane in two consecutive steps facilitated by the Sec translocon and the Bam complex, respectively. In this mini-review we discuss how autotransporters translocate their secreted functional domains across the outer membrane. We highlight the interactions with the Bam complex and discuss how specific features of the recently solved structure of Bam lead to a mechanistic model for autotransporter secretion...
September 1, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/30064508/socio-demographic-characteristics-and-the-utilization-of-hiv-testing-and-counselling-services-among-the-key-populations-at-the-bhutanese-refugees-camps-in-eastern-nepal
#14
Salina Khatoon, Shyam Sundar Budhathoki, Kiran Bam, Rajshree Thapa, Lokesh P Bhatt, Bidhya Basnet, Nilambar Jha
OBJECTIVES: This cross-sectional study was conducted to describe the socio-demographic characteristics, assess the utilization of HIV testing and counselling services, and to explore the reasons for the non-utilization of HIV testing and counselling services among the key populations at the Bhutanese refugee camps in eastern Nepal. RESULTS: The HIV testing and counselling services are utilized by less than a third (29%) of the key population among the Bhutanese Refugees...
July 31, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/30059852/phase-behavior-of-dspc-peg-40-st-mixtures-at-higher-emulsifier-contents
#15
Sevgi Kilic, Elif Seniz Bolukcu
Phase behaviors of 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) and polyoxyethylene(40)stearate (PEG40 St) were investigated with Langmuir monolayer isotherms and Brewster angle microscopy (BAM) imaging at DSPC/PEG40 St molar ratios ranging from 9:1 to 5:5. Two plateaus were found in the Langmuir isotherms which were relatively shorter for the 9:1 mixture and extended significantly by increasing the PEG40 St content, indicating that the PEG40 St squeezed out whereas more emulsifier retained in the monolayer at higher PEG40 St contents...
July 24, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30057728/exciting-perspectives-for-translational-myology-in-the-abstracts-of-the-2018spring-paduamuscledays-giovanni-salviati-memorial-chapter-iv-abstracts-of-march-17-2018
#16
Ugo Carraro
Myologists working in Padua (Italy) were able to continue a half-century tradition of studies of skeletal muscles, that started with a research on fever, specifically if and how skeletal muscle contribute to it by burning bacterial toxin. Beside main publications in high-impact-factor journals by Padua myologists, I hope to convince readers (and myself) of the relevance of the editing Basic and Applied Myology (BAM), retitled from 2010 European Journal of Translational Myology (EJTM), of the institution of the Interdepartmental Research Center of Myology of the University of Padova (CIR-Myo), and of a long series of International Conferences organized in Euganei Hills and Padova, that is, the PaduaMuscleDays...
January 12, 2018: European Journal of Translational Myology
https://www.readbyqxmd.com/read/30057727/exciting-perspectives-for-translational-myology-in-the-abstracts-of-the-2018spring-paduamuscledays-giovanni-salviati-memorial-chapter-iii-abstracts-of-march-16-2018
#17
Ugo Carraro
Myologists working in Padua (Italy) were able to continue a half-century tradition of studies of skeletal muscles, that started with a research on fever, specifically if and how skeletal muscle contribute to it by burning bacterial toxin. Beside main publications in high-impact-factor journals by Padua myologists, I hope to convince readers (and myself) of the relevance of the editing Basic and Applied Myology (BAM), retitled from 2010 European Journal of Translational Myology (EJTM), of the institution of the Interdepartmental Research Center of Myology of the University of Padova (CIR-Myo), and of a long series of International Conferences organized in Euganei Hills and Padova, that is, the PaduaMuscleDays...
January 12, 2018: European Journal of Translational Myology
https://www.readbyqxmd.com/read/30057726/exciting-perspectives-for-translational-myology-in-the-abstracts-of-the-2018spring-paduamuscledays-giovanni-salviati-memorial-chapter-ii-abstracts-of-march-15-2018
#18
Ugo Carraro
Myologists working in Padua (Italy) were able to continue a half-century tradition of studies of skeletal muscles, that started with a research on fever, specifically if and how skeletal muscle contribute to it by burning bacterial toxin. Beside main publications in high-impact-factor journals by Padua myologists, I hope to convince readers (and myself) of the relevance of the editing Basic and Applied Myology (BAM), retitled from 2010 European Journal of Translational Myology (EJTM), of the institution of the Interdepartmental Research Center of Myology of the University of Padova (CIR-Myo), and of a long series of International Conferences organized in Euganei Hills and Padova, that is, the PaduaMuscleDays...
January 12, 2018: European Journal of Translational Myology
https://www.readbyqxmd.com/read/30056937/mesoscopic-characterization-of-amphiphilic-monoglycerol-monolayers
#19
REVIEW
D Vollhardt
The introduction of the highly-sensitive imaging technique Brewster angle microscopy (BAM) has given rise to new knowledges about the mesoscopic topology and ordering of condensed phase domains formed in the two-phase coexistence region of Langmuir monolayers. Besides fatty acids, monoalkanoylglycerols are the most studied amphiphiles at the air-water interface. In this review, the mesoscopic characterization of amphiphilic monoglycerol monolayers is surveyed to demonstrate the striking effect of the position of the glycerol backbone at which the polar head group is substituted...
August 2018: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/30049980/disaster-management-system-aided-by-named-data-network-of-things-architecture-design-and-analysis
#20
Abdul Hannan, Sobia Arshad, Muhammad Awais Azam, Jonathan Loo, Syed Hassan Ahmed, Muhammad Faran Majeed, Sayed Chhattan Shah
Disasters are the uncertain calamities which within no time can change the situation quite drastically. They not only affect the system's infrastructure but can also put an adverse effect on human life. A large chunk of the IP-based Internet of Things (IoT) schemes tackle disasters such as fire, earthquake, and flood. Moreover, recently proposed Named Data Networking (NDN) architecture exhibited promising results for IoT as compare to IP-based approaches. Therefore to tackle disaster management system (DMS), it is needed to explore it through NDN architecture and this is the main motivation behind this work...
July 26, 2018: Sensors
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