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https://www.readbyqxmd.com/read/28926262/effect-of-the-incorporation-of-nano-sized-titanium-dioxide-on-the-interfacial-properties-of-dppc-langmuir-monolayers
#1
Eduardo Guzmán, Eva Santini, Michele Ferrari, Libero Liggieri, Francesca Ravera
The effect of the incorporation of hydrophilic titanium dioxide (TiO2) nanoparticles on the interfacial properties of Langmuir monolayers of 1,2-Dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) has been evaluated combining interfacial thermodynamic studies, dilatational rheology and Brewster Angle Microscopy (BAM). The results show that the TiO2 nanoparticles are able to penetrate into DPPC layers, modifying the organization of the molecules and, consequently, the phase behaviour and viscoelastic properties of the systems...
September 19, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28922129/dissipativity-and-synchronization-of-generalized-bam-neural-networks-with-multivariate-discontinuous-activations
#2
Dongshu Wang, Lihong Huang, Longkun Tang
This paper is concerned with the dissipativity and synchronization problems of a class of delayed bidirectional associative memory (BAM) neural networks in which neuron activations are modeled by discontinuous bivariate functions. First, the concept of the Filippov solution is extended to functional differential equations with discontinuous right-hand sides and mixed delays via functional differential inclusions. The global dissipativity of the Filippov solution to the considered BAM neural networks is proven using generalized Halanay inequalities and matrix measure approaches...
September 14, 2017: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/28919234/effects-of-periplasmic-chaperones-and-membrane-thickness-on-bama-catalysed-outer-membrane-protein-folding
#3
Bob Schiffrin, Antonio N Calabrese, Anna J Higgins, Julia R Humes, Alison E Ashcroft, Antreas C Kalli, David J Brockwell, Sheena E Radford
The biogenesis of outer membrane proteins (OMPs) in Gram-negative bacteria involves delivery by periplasmic chaperones to the β-barrel assembly machinery (BAM) which catalyses OMP insertion into the outer membrane (OM). Here, we examine the effects of membrane thickness, the E. coli periplasmic chaperones Skp and SurA, and BamA, the central subunit of the BAM complex, on the folding kinetics of a model OMP (tOmpA) using fluorescence spectroscopy, native mass spectrometry, and molecular dynamics (MD) simulations...
September 15, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28902119/determination-of-vertical-jump-as-a-measure-of-neuromuscular-readiness-and-fatigue
#4
Casey M Watkins, Saldiam R Barillas, Megan A Wong, David C Archer, Ian J Dobbs, Robert G Lockie, Jared W Coburn, Tai T Tran, Lee E Brown
Coaches closely monitor training loads and periodize sessions throughout the season to create optimal adaptations at the proper time. However, only monitoring training loads ignores the innate physiological stress each athlete feels individually. Vertical jump (VJ) is widely used as a measure of lower body power, and has been used in post-match studies to demonstrate fatigue levels. However, no pre-training monitoring via VJ performance has been previously studied. Therefore, the purpose of this study was to determine the sensitivity of VJ as a measure of readiness and fatigue on a daily sessional basis...
September 6, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28901288/multiple-conserved-cell-adhesion-protein-interactions-mediate-neural-wiring-of-a-sensory-circuit-in-c-elegans
#5
Byunghyuk Kim, Scott W Emmons
Nervous system function relies on precise synaptic connections. A number of widely-conserved cell adhesion proteins are implicated in cell recognition between synaptic partners, but how these proteins act as a group to specify a complex neural network is poorly understood. Taking advantage of known connectivity in C. elegans, we identified and studied cell adhesion genes expressed in three interacting neurons in the mating circuits of the adult male. Two interacting pairs of cell surface proteins independently promote fasciculation between sensory neuron HOA and its postsynaptic target interneuron AVG: BAM-2/neurexin-related in HOA binds to CASY-1/calsyntenin in AVG; SAX-7/L1CAM in sensory neuron PHC binds to RIG-6/contactin in AVG...
September 13, 2017: ELife
https://www.readbyqxmd.com/read/28894353/optimized-next-generation-sequencing-genotype-haplotype-calling-for-genome-variability-analysis
#6
Javier Navarro, Bruno Nevado, Porfidio Hernández, Gonzalo Vera, Sebastián E Ramos-Onsins
The accurate estimation of nucleotide variability using next-generation sequencing data is challenged by the high number of sequencing errors produced by new sequencing technologies, especially for nonmodel species, where reference sequences may not be available and the read depth may be low due to limited budgets. The most popular single-nucleotide polymorphism (SNP) callers are designed to obtain a high SNP recovery and low false discovery rate but are not designed to account appropriately the frequency of the variants...
2017: Evolutionary Bioinformatics Online
https://www.readbyqxmd.com/read/28887826/job-contenders-roles-of-the-%C3%AE-barrel-assembly-machinery-and-the-translocation-and-assembly-module-in-autotransporter-secretion
#7
REVIEW
Cécile Albenne, Raffaele Ieva
In Gram-negative bacteria, autotransporters secrete effector protein domains that are linked to virulence. Although they were once thought to be simple and autonomous secretion machines, mounting evidence reveals that multiple factors of the bacterial envelope are necessary for autotransporter assembly. Secretion across the outer membrane of their soluble effector "passenger domain" is promoted by the assembly of an outer membrane-spanning "β-barrel domain". Both reactions require BamA, an essential component of the β-barrel assembly machinery (BAM complex) that catalyzes the final reaction step by which outer membrane proteins are integrated into the lipid bilayer...
September 8, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28886680/outer-membrane-biogenesis
#8
Anna Konovalova, Daniel E Kahne, Thomas J Silhavy
The hallmark of gram-negative bacteria and organelles such as mitochondria and chloroplasts is the presence of an outer membrane. In bacteria such as Escherichia coli, the outer membrane is a unique asymmetric lipid bilayer with lipopolysaccharide in the outer leaflet. Integral transmembrane proteins assume a β-barrel structure, and their assembly is catalyzed by the heteropentameric Bam complex containing the outer membrane protein BamA and four lipoproteins, BamB-E. How the Bam complex assembles a great diversity of outer membrane proteins into a membrane without an obvious energy source is a particularly challenging problem, because folding intermediates are predicted to be unstable in either an aqueous or a hydrophobic environment...
September 8, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28880192/global-exponential-stability-of-impulsive-fuzzy-high-order-bam-neural-networks-with-continuously-distributed-delays
#9
Wengui Yang, Wenwu Yu, Jinde Cao
This paper investigates the stability of equilibrium point and periodic solution for impulsive fuzzy high-order bidirectional associative memory neural networks with continuously distributed delays. By applying the inequality analysis technique, $M$-matrix, and Banach contraction mapping principle and constructing some suitable Lyapunov functionals, some sufficient conditions for the uniqueness and global exponential stability of equilibrium point and global exponential stability of periodic solutions are established...
August 31, 2017: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/28872368/cryotherapy-re-invented-application-of-phase-change-material-for-recovery-in-elite-soccer
#10
Tom Clifford, Will Abbott, Susan Y Kwiecien, Glyn Howatson, Malachy P McHugh
PURPOSE: This study examined whether donning lower body garments fitted with cooled phase change material (PCM) would enhance recovery after a soccer match. METHODS: In a randomized, crossover design, eleven elite-soccer players from the reserve squad of a team in the 2nd highest league in England wore PCM cooled to 15°C (PCMcold) or left at ambient temperature (PCMamb; sham control) for 3 h after a soccer match. To assess recovery, countermovement jump (CMJ) height, maximal isometric voluntary contraction (MIVC), muscle soreness (MS), and the adapted Brief Assessment of Mood Questionnaire (BAM+) were measured before, 12, 36 and 60 h after each match...
September 5, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28858422/development-and-psychometric-properties-of-the-basic-amputee-mobility-score-for-use-in-patients-with-a-major-lower-extremity-amputation
#11
Morten Tange Kristensen, Anni Østergaard Nielsen, Ulla Madsen Topp, Jakob Holmehave-Brandt, Charlotte Falkenberg Petterson, Peter Gebuhr
AIM: To develop and examine the psychometric properties, including responsiveness and interrater reliability, of a new outcome measure for the evaluation of basic mobility activities after a major lower extremity amputation - The Basic Amputee Mobility Score (BAMS). METHODS: The four following essential activities were chosen through consensus meetings with experienced amputee physiotherapists: (i) supine in bed to sitting on the edge of the bed; (ii) bed to wheelchair transfer; (iii) indoor wheelchair mobility; and (iv) get up from a wheelchair to standing on the non-amputated leg...
August 31, 2017: Geriatrics & Gerontology International
https://www.readbyqxmd.com/read/28831113/rna-seq-reveals-microrna-expression-signature-and-genetic-polymorphism-associated-with-growth-and-muscle-quality-traits-in-rainbow-trout
#12
Bam Dev Paneru, Rafet Al-Tobasei, Brett Kenney, Timothy D Leeds, Mohamed Salem
The role of microRNA expression and genetic variation in microRNA-binding sites of target genes on growth and muscle quality traits is poorly characterized. We used RNA-Seq approach to investigate their importance on 5 growth and muscle quality traits: whole body weight (WBW), muscle yield, muscle crude-fat content, muscle shear force and whiteness. Phenotypic data were collected from 471 fish, representing 98 families (~5 fish/family) from a growth-selected line. Muscle microRNAs and mRNAs were sequenced from 22 families showing divergent phenotypes...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28829718/development-and-field-validation-of-a-community-engaged-particulate-matter-air-quality-monitoring-network-in-imperial-ca
#13
Graeme N Carvlin, Humberto Lugo, Luis Olmedo, Ester Bejarano, Alexa Wilkie, Dan Meltzer, Michelle Wong, Galatea King, Amanda Northcross, Michael Jerrett, Paul B English, Donald Hammond, Edmund Seto
The Imperial County Community Air Monitoring Network was developed as part of a community-engaged research study to provide real-time particulate matter (PM) air quality information at a high spatial resolution in Imperial County, California. The network augmented the few existing regulatory monitors and increased monitoring near susceptible populations. Monitors were both calibrated and field validated, a key component of evaluating the quality of the data produced by the community-monitoring network. This paper examines the performance of a customized version of the low-cost Dylos optical particle counter used in the community air monitors compared to both PM2...
August 22, 2017: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/28807615/interfacial-properties-of-avian-stratum-corneum-monolayers-investigated-by-brewster-angle-microscopy-and-vibrational-sum-frequency-generation
#14
Ellen M Adams, Alex M Champagne, Joseph B Williams, Heather C Allen
The outermost layer of skin, the stratum corneum (SC), contains a complex mixture of lipids, which controls the rate of cutaneous water loss (CWL) in reptiles, mammals, and birds. However, the molecular structure of SC lipids and how molecular configurations influence CWL is poorly understood. Here, the organization and structure of SC lipids extracted from birds were investigated by means of Langmuir films. Properties of lipids from the SC of arid and semi-arid adapted larks, known to have a low CWL, were compared with lipids extracted from the SC of mesic lark species with higher CWL to gain insight into how structure impacts CWL...
August 12, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28800823/detection-and-quantification-of-alicyclobacillus-acidoterrestris-by-electrical-impedance-in-apple-juice
#15
Pilar Fernández, José Antonio Gabaldón, Mª Jesús Periago
Alicyclobacillus acidoterrestris is a thermotolerant bacterium able to grow in fruit juices and drinks, as the spoilage by Alicyclobacillus in the final product does not product any gas, but leads to a "medicine flavor" due to the formation of guaicol. Also, its detection is a challenge for the quality control departments, because it takes several days to get the results of traditional microbiology methods. This study aimed at developing a more accurate electrical impedance technique for the detection of A...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28791869/study-of-compression-induced-supramolecular-nanostructures-of-an-imidazole-derivative-by-langmuir-blodgett-technique
#16
Bapi Dey, Pintu Debnath, Santanu Chakraborty, Barnali Deb, Debajyoti Bhattacharjee, Swapan Majumdar, Syed Arshad Hussain
In this communication, we report the design and synthesis as well as the supramolecular assembly behavior of a 2,4,5-triaryl imidazole derivative (compound 1) at the air-water interface and in thin films using Langmuir-Blodgett (LB) technique. The main idea for such a chemical structure is that the long alkyl chain and N-H of the imidazole core may help to form supramolecular architecture through the hydrophobic-hydrophobic interaction and hydrogen bonding, respectively. Accordingly, the interfacial behavior as well as morphology of 1 in thin films were studied through a series of characterization methods such as surface pressure-area (π-A) isotherm, hysteresis analysis, ultraviolet-visible (UV-vis) absorption and steady-state fluorescence spectroscopies, Fourier transform infrared, X-ray diffraction, Brewster angle microscopy (BAM), and atomic force microscopy (AFM) measurements, and so forth...
August 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28783065/assessing-the-utility-of-low-cost-particulate-matter-sensors-over-a-12-week-period-in-the-cuyama-valley-of-california
#17
Anondo Mukherjee, Levi G Stanton, Ashley R Graham, Paul T Roberts
The use of low-cost air quality sensors has proliferated among non-profits and citizen scientists, due to their portability, affordability, and ease of use. Researchers are examining the sensors for their potential use in a wide range of applications, including the examination of the spatial and temporal variability of particulate matter (PM). However, few studies have quantified the performance (e.g., accuracy, precision, and reliability) of the sensors under real-world conditions. This study examined the performance of two models of PM sensors, the AirBeam and the Alphasense Optical Particle Counter (OPC-N2), over a 12-week period in the Cuyama Valley of California, where PM concentrations are impacted by wind-blown dust events and regional transport...
August 5, 2017: Sensors
https://www.readbyqxmd.com/read/28778052/interaction-of-levofloxacin-with-lung-surfactant-at-the-air-water-interface
#18
Stephanie Ortiz-Collazos, Evelina D Estrada-López, Alline A Pedreira, Paulo H S Picciani, Osvaldo N Oliveira, Andre S Pimentel
The molecular-level interaction of levofloxacin with lung surfactant was investigated using Langmuir monolayers and atomistic molecular dynamics (MD) simulations. In the simulation, the DPPC/POPC mixed monolayer was used as a lung surfactant model and the molecules of levofloxacin were placed at the air-lipid interface to mimic the adsorption process on the lung surfactant model. The simulation results indicate that amphoteric levofloxacin expands the lung surfactant, also stabilizing the film for levofloxacin fractions until 10% w/w at least...
July 27, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28774613/monohalogenated-acetamide-induced-cellular-stress-and-genotoxicity-are-related-to-electrophilic-softness-and-thiol-thiolate-reactivity
#19
Justin A Pals, Elizabeth D Wagner, Michael J Plewa, Menghang Xia, Matias S Attene-Ramos
Haloacetamides (HAMs) are cytotoxic, genotoxic, and mutagenic byproducts of drinking water disinfection. They are soft electrophilic compounds that form covalent bonds with the free thiol/thiolate in cysteine residues through an SN2 reaction mechanism. Toxicity of the monohalogenated HAMs (iodoacetamide, IAM; bromoacetamide, BAM; or chloroacetamide, CAM) varied depending on the halogen substituent. The aim of this research was to investigate how the halogen atom affects the reactivity and toxicological properties of HAMs, measured as induction of oxidative/electrophilic stress response and genotoxicity...
August 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28765033/assessment-of-butorphanol-azaperone-medetomidine-combination-as-anesthesia-for-semen-collection-and-evaluation-of-semen-quality-in-white-tailed-deer-odocoileus-virginianus
#20
S M Kirschner, R Rodenkirch
The aim of this current study was to evaluate the level of anesthesia produced by a combination of butorphanol-azaperone-medetomidine (BAM) for semen collection by electroejaculation on captive white-tailed bucks (Odocoileus virginianus). Ten male white-tailed deer, weighing 68.2-115.9kg, ranging in age from one to four years were randomly selected from housing pens and anesthetized with the BAM drug combination at a dose volume of 2.0mL each. Semen was collected from each animal using a standard cervid electroejaculation protocol while under BAM anesthesia...
July 25, 2017: Animal Reproduction Science
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