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David A Shearer, William Sparkes, Jonny Northeast, Daniel J Cunningham, Christian J Cook, Liam P Kilduff
OBJECTIVES: Biochemical (e.g. creatine kinase (CK)) and neuromuscular (e.g. peak power output (PPO)) markers of recovery are expensive and require specialist equipment. Perceptual measures are an effective alternative, yet most validated scales are too long for daily use. DESIGN: This study utilises a longitudinal multi-level design to test an adapted Brief Assessment of Mood (BAM+), with four extra items and a 100mm visual analogue scale to measure recovery. METHODS: Elite under-21 academy soccer players (N=11) were monitored across five games with data (BAM+, CK and PPO) collected for each game at 24h pre, 24h and 48h post-match...
October 5, 2016: Journal of Science and Medicine in Sport
Nitin Chaubal, Mukund Joshi, Anupam Bam, Rajas Chaubal
No abstract text is available yet for this article.
October 2016: Seminars in Roentgenology
Xicheng Wang, Zhengyan Zhao, Quansheng Wu, Yanyan Li, Yuhua Wang
A series of Ca2YZr2Al3O12:Eu(3+) (CYZA) phosphors were successfully synthesized through conventional solid-state method. The electronic structure and their photoluminescence or cathodoluminescence properties were investigated in detail. Under n-UV excitation, the CYZA:Eu(3+) exhibits more intense red emission than the commercial Y2O3:Eu(3+) phosphor. A WLED lamp with good color render index was obtained by fabricating the phosphor with BAM:Eu(2+) and LuAG:Ce(3+) phosphors. The phosphor also exhibits red emission with high current saturation and high resistance under low voltage electron bombardment...
October 12, 2016: Inorganic Chemistry
Neeraj Nagpal
Physicians and Internists in India have tended to brush under the carpet legal issues affecting their profession. Of concern to all Physicians is the judgment in a recent case where the NCDRC has stated that if MD Medicine Physicians write Physician & Cardiologist on their letterhead it is Quackery. What is MD Medicine degree holder in India qualified and trained to treat ? These are issues which need debate and that can only be initiated once we recognize that there is a problem. Either an MD Medicine is a cardiologist or he is not...
February 2016: Journal of the Association of Physicians of India
Xing Ma, Yingying Han, Xiaoqing Song, Trieu Do, Zhihao Yang, Jianquan Ni, Ting Xie
Stem cells in adult tissues are constantly exposed to genotoxic stress and also accumulate DNA damage with age. DNA damage has been proposed to cause stem cell loss and cancer formation. However, it remains a mystery how DNA damage leads to both stem cell loss and cancer formation. In this study, we use germline stem cells (GSCs) in the Drosophila ovary to show that DNA damage retards stem cell self-renewal and lineage differentiation in a CHK2 kinase-dependent manner. Both heatshock-inducible endonuclease I-CreI expression and X-ray irradiation can efficiently introduce double-strand breaks in GSCs and their progeny, resulting in a rapid GSC loss and an accumulation of ill-differentiated GSC progeny...
October 11, 2016: Development
Marta Arczewska, Grzegorz Czernel, Mariusz Gagos
In this work, a comparative study on the effect of an antibiotic amphotericin B (AmB) and its copper complex on the multicomponent monolayers imitating the outer leaflet of human erythrocyte membrane has been performed by means of the Langmuir monolayer technique. The properties of mixed films were analysed by thermodynamic description of the interactions between the molecules, complemented with the morphology of monolayers established by Brewster Angle Microscopy (BAM). The results revealed differences in the molecular organisation of the two antibiotic forms at the air/water interface, which were explained by the different spatial structure of the complex...
October 10, 2016: Journal of Physical Chemistry. B
Behrooz Hashemi, Saeed Safari, Mostafa Hosseini, Mahmoud Yousefifard, Elham Erfani, Alireza Baratloo, Farhad Rahmati, Maryam Motamedi, Mohammad Mehdi Forouzanfar, Iraj Najafi
CONTEXT: Crush syndrome and its potentially life-threatening complications, such as acute kidney injury (AKI), are one of the most important medical problems of disaster victims. However, today, many unanswered questions abound about the potential risk factors of crush syndrome, predictive factors of AKI, proper amount of prophylactic hydration therapy, type of fluid, time of continuing fluid, intravenous versus oral hydration, etc. Therefore, this study was designed to review the findings on Iranian nephrologist experiences in diagnosis and management of traumatic rhabdomyolysis following the last two strong earthquakes of Bam (2003) and Manjil-Rudbar (1990)...
June 2016: Archives of Trauma Research
Hoang T Nguyen, James Boocock, Tony R Merriman, Michael A Black
Copy-number variation (CNV) has been associated with increased risk of complex diseases. High-throughput sequencing (HTS) technologies facilitate the detection of copy-number variable regions (CNVRs) and their breakpoints. This helps in understanding genome structure as well as their evolution process. Various approaches have been proposed for detecting CNV breakpoints, but currently it is still challenging for tools based on a single analysis method to identify breakpoints of CNVs. It has been shown, however, that pipelines which integrate multiple approaches are able to report more reliable breakpoints...
2016: Frontiers in Genetics
Matthew G Iadanza, Anna J Higgins, Bob Schiffrin, Antonio N Calabrese, David J Brockwell, Alison E Ashcroft, Sheena E Radford, Neil A Ranson
The β-barrel assembly machinery (BAM) is a ∼203 kDa complex of five proteins (BamA-E), which is essential for viability in E. coli. BAM promotes the folding and insertion of β-barrel proteins into the outer membrane via a poorly understood mechanism. Several current models suggest that BAM functions through a 'lateral gating' motion of the β-barrel of BamA. Here we present a cryo-EM structure of the BamABCDE complex, at 4.9 Å resolution. The structure is in a laterally open conformation showing that gating is independent of BamB binding...
2016: Nature Communications
E Y Yakushev, E A Mikhaleva, Y A Abramov, O A Sokolova, I M Zyrianova, V A Gvozdev, M S Klenov
The Piwi protein and its orthologs are considered as the key components of the piRNA machinery implicated in transcriptional silencing of transposons. Неre, we show that nuclear localization of the Piwi protein is required not only for transposon repression, but also for proper differentiation of germline stem cells (GSCs). piwi^(Nt) mutation that causes loss of nuclear Piwi and its retention in the cytoplasm leads to the accumulation of undifferentiated GSC-like cells. The analysis of piwi^(Nt) mutation in combination with a bam gene mutation blocking GSC differentiation shows that the loss of nuclear Piwi decreases GSC proliferation rate...
July 2016: Molekuliarnaia Biologiia
Bayo Lau, Marghoob Mohiyuddin, John C Mu, Li Tai Fang, Narges Bani Asadi, Carolina Dallett, Hugo Y K Lam
: LongISLND is a software package designed to simulate sequencing data according to the characteristics of third generation, single-molecule sequencing technologies. The general software architecture is easily extendable, as demonstrated by the emulation of Pacific Biosciences (PacBio) multi-pass sequencing with P5 and P6 chemistries, producing data in FASTQ, H5, and the latest PacBio BAM format. We demonstrate its utility by downstream processing with consensus building and variant calling...
September 25, 2016: Bioinformatics
Manuel Tsiang, Gregg S Jones, Joshua Goldsmith, Andrew Mulato, Derek Hansen, Elaine Kan, Luong Tsai, Rujuta A Bam, George Stepan, Kirsten M Stray, Anita Niedziela-Majka, Stephen R Yant, Helen Yu, George Kukolj, Tomas Cihlar, Scott Lazerwith, Kirsten L White, Haolun Jin
Bictegravir (BIC; GS-9883), a novel potent, once-daily, unboosted inhibitor of HIV-1 integrase (IN) specifically targets IN strand transfer activity (IC50 = 7.5 ± 0.3 nM) and HIV-1 integration in cells. BIC exhibits potent and selective in vitro antiretroviral activity in both T-cell lines and primary human T-lymphocytes with EC50 values ranging from 1.5 to 2.4 nM and selectivity indices up to 8800 relative to cytotoxicity. BIC exhibits synergistic in vitro antiviral effects in pairwise combinations with tenofovir alafenamide, emtricitabine or darunavir and maintains potent antiviral activity against HIV-1 variants resistant to other classes of antiretrovirals...
September 19, 2016: Antimicrobial Agents and Chemotherapy
Dadi Jiang, Connor Lynch, Bruno C Medeiros, Michaela Liedtke, Rakesh Bam, Arvin B Tam, Zhifen Yang, Muthuraman Alagappan, Parveen Abidi, Quynh-Thu Le, Amato J Giaccia, Nicholas C Denko, Maho Niwa, Albert C Koong
Activation of the IRE1α-XBP1 branch of the unfolded protein response (UPR) has been implicated in multiple types of human cancers, including multiple myeloma (MM). Through an in silico drug discovery approach based on protein-compound virtual docking, we identified the anthracycline antibiotic doxorubicin as an in vitro and in vivo inhibitor of XBP1 activation, a previously unknown activity for this widely utilized cancer chemotherapeutic drug. Through a series of mechanistic and phenotypic studies, we showed that this novel activity of doxorubicin was not due to inhibition of topoisomerase II (Topo II)...
2016: Scientific Reports
Sophie Melikishvili, Alexandra Poturnayova, Maksim Ionov, Maria Bryszewska, Tomáš Vary, Julius Cirak, María Ángeles Muñoz-Fernández, Rafael Gomez-Ramirez, Francisco Javier de la Mata, Tibor Hianik
In this study, dendrimers have been purposed as an alternative approach for delivery of HIV peptides to dendritic cells. We have investigated the interaction of dendriplexes formed from polyanionic HIV peptide Nef and cationic carbosilane dendrimer (CBD) with model lipid membranes - large unilamellar vesicles (LUVs), Langmuir monolayers and supported lipid membranes (sBLMs) containing various molar ratio of zwitterionic 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG2000)...
September 9, 2016: Biochimica et Biophysica Acta
Christopher Stubenrauch, Rhys Grinter, Trevor Lithgow
Diderm bacteria have an outer membrane that provides defense against environmental factors including antibiotics. Understanding the process of outer membrane biogenesis is, therefore, of critical importance in order to envisage new treatments of these bacterial pathogens. Borrelia burgdorferi is the pathogen responsible for Lyme disease. Its outer membrane contains integral, β-barrel proteins as well as swathes of externally exposed lipoproteins. Previous work has demonstrated that the β-barrel assembly machine (BAM complex) in B...
September 12, 2016: Molecular Microbiology
Zhuoyi Huang, Navin Rustagi, Narayanan Veeraraghavan, Andrew Carroll, Richard Gibbs, Eric Boerwinkle, Manjunath Gorentla Venkata, Fuli Yu
BACKGROUND: The decreasing costs of sequencing are driving the need for cost effective and real time variant calling of whole genome sequencing data. The scale of these projects are far beyond the capacity of typical computing resources available with most research labs. Other infrastructures like the cloud AWS environment and supercomputers also have limitations due to which large scale joint variant calling becomes infeasible, and infrastructure specific variant calling strategies either fail to scale up to large datasets or abandon joint calling strategies...
2016: BMC Bioinformatics
Abhinav Nellore, Leonardo Collado-Torres, Andrew E Jaffe, José Alquicira-Hernández, Christopher Wilks, Jacob Pritt, James Morton, Jeffrey T Leek, Ben Langmead
MOTIVATION: RNA sequencing (RNA-seq) experiments now span hundreds to thousands of samples. Current spliced alignment software is designed to analyze each sample separately. Consequently, no information is gained from analyzing multiple samples together, and it requires extra work to obtain analysis products that incorporate data from across samples. RESULTS: We describe Rail-RNA, a cloud-enabled spliced aligner that analyzes many samples at once. Rail-RNA eliminates redundant work across samples, making it more efficient as samples are added...
September 4, 2016: Bioinformatics
Henna Iqbal, Melisha R Kenedy, Meghan Lybecker, Darrin R Akins
Two outer membrane protein (OMP) transport systems in diderm bacteria assist in assembly and export of OMPs. These two systems are the β-barrel assembly machine (BAM) complex and the translocation and assembly module (TAM). The BAM complex consists of the OMP component BamA along with several outer membrane associated proteins. The TAM also consists of an OMP, designated TamA, and a single inner membrane (IM) protein, TamB. Together TamA and TamB aid in the secretion of virulence-associated OMPs. In this study we characterized the hypothetical protein BB0794 in Borrelia burgdorferi...
September 2, 2016: Molecular Microbiology
Shinji Sakai, Koujiro Nomura, Kei Mochizuki, Masahito Taya
Polyethylene glycol (PEG) derivatives possessing oleyl and reactive groups for conjugating functional substrates, such as proteins and quantum dots, are useful materials for cell-surface engineering and cell immobilization onto substrates. The reagent is known as a biocompatible anchor for cell membranes (BAM). Here, BAM-anchoring on cell membranes is reported to stimulate reactive oxygen species (ROS) production in those cells. Significant increases in ROS production and release to the surrounding environment were detected in mouse fibroblast cell line 10T1/2 when soaked in a solution containing BAM conjugated with 1/10mol/mol bovine serum albumin at 1...
November 1, 2016: Colloids and Surfaces. B, Biointerfaces
Rodrigo Cánovas, Alistair Moffat, Andrew Turpin
MOTIVATION: Next generation sequencing machines produce vast amounts of genomic data. For the data to be useful, it is essential that it can be stored and manipulated efficiently. This work responds to the combined challenge of compressing genomic data, while providing fast access to regions of interest, without necessitating decompression of whole files. RESULTS: We describe CSAM (Compressed SAM format), a compression approach offering lossless and lossy compression for SAM files...
August 18, 2016: Bioinformatics
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