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Liene Smane, Mara Pilmane
Cleft lip and palate (CLP) is the most common defect affecting the face. The treatment consists of surgical reconstruction of the anatomical structures of the cleft. Part of the surgical treatment is reconstruction of the alveolar bone by means of autogenic bone grafting (osteoplasty). This study aimed to evaluate the levels of expression of extracellular matrix remodeling factors in the facial tissue of children with a complete unilateral (CU) and a complete bilateral (CB) CLP to assess whether the wound healing process is adequate...
2018: Acta Medica Lituanica
Chunxia Yu, Weimin Wang, Xin Jin
BACKGROUND Myocardial fibrosis is closely related to all types of cardiovascular diseases. Hirudin is widely used in the prevention and treatment of cardiovascular diseases and cancers. In this study, we examined the potential role(s) and mechanism of hirudin in angiotensin II (Ang II)-induced myocardial fibrosis. MATERIAL AND METHODS The viability of myocardial fibroblasts, and reactive oxygen species (ROS) rates were measured respectively using cell counting kit-8 (CCK-8) and flow cytometry. Malondialdehyde (MDA) content, the activities of lactate dehydrogenase (LDH), and superoxide dismutase (SOD) were detected by the respective kits...
September 8, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Nathan M Lee, Lev Deriy, Timothy R Petersen, Vallabh O Shah, Michael P Hutchens, Neal S Gerstein
OBJECTIVE: Administration of excess chloride in 0.9% normal saline (NS) decreases renal perfusion and glomerular filtration rate, thereby increasing the risk for acute kidney injury (AKI). In this study, the effect of NS versus Isolyte use during cardiac surgery on urinary levels of tissue inhibitor of metalloproteinase 2 and insulin-like growth factor-binding protein 7 [TIMP-2] × [IGFBP7] and postoperative risk of AKI were examined. DESIGN: Prospective, randomized, and single-blinded trial...
August 1, 2018: Journal of Cardiothoracic and Vascular Anesthesia
Weixuan Fan, Ghada Ankawi, Jingxiao Zhang, Kumar Digvijay, Davide Giavarina, Yongjie Yin, Claudio Ronco
No abstract text is available yet for this article.
September 4, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Hongya Guan, Yingwu Mei, Yang Mi, Cheng Li, Xiaoya Sun, Xuefeng Zhao, Jia Liu, Wei Cao, Yuebai Li, Yisheng Wang
Background: This study was designed to research the potential function of lncRNA ANRIL in osteosarcoma (OS). Materials and methods: Quantitative real-time PCR, cell counting kit-8, wound healing assay, Transwell assay, flow cytometric analysis, caspase activity analysis, and Western blot were carried out. Results: ANRIL was remarkably upregulated in human OS tissues and cells, and knockdown of ANRIL significantly suppressed MG63 cell proliferation, migration, and invasion and promoted apoptosis...
2018: OncoTargets and Therapy
Charles Hobson, Nicholas Lysak, Matthew Huber, Salvatore Scali, Azra Bihorac
OBJECTIVE: Conventional clinical wisdom has often been nihilistic regarding the prevention and management of acute kidney injury (AKI), despite its being a frequent and morbid complication associated with both increased mortality and cost. Recent developments have shown that AKI is not inevitable and that changes in management of patients can reduce both the incidence and morbidity of perioperative AKI. The purpose of this narrative review was to review the epidemiology and outcomes of AKI in patients undergoing vascular surgery using current consensus definitions, to discuss some of the novel emerging risk stratification and prevention techniques relevant to the vascular surgery patient, and to describe a standardized perioperative pathway for the prevention of AKI after vascular surgery...
September 2018: Journal of Vascular Surgery
Yusuf Yilmaz, Fatih Eren
BACKGROUND: We sought to explore the interplay of multiple serum biomarkers of fibrosis and extracellular matrix remodeling with the results of liver histology in patients with nonalcoholic fatty liver disease (NAFLD). PATIENTS AND METHODS: Venous blood samples were collected from 80 patients with biopsy-proven NAFLD and 59 age-matched and sex-matched healthy controls. Serum levels of transforming growth factor (TGF)-β1, TGF-β2, matrix metalloproteinases (MMP)-1, MMP-2, MMP-7, MMP-9, MMP-10, tissue inhibitors of metalloproteinase (TIMP)-1, and TIMP-2 were determined by using the Luminex MagPix technology on a MAGPIX analyzer...
August 21, 2018: European Journal of Gastroenterology & Hepatology
Xuezhi Zhao, Xiaohan Guo, Junhua Shen, Dingchao Hua
Natural bioactive components are increasingly being applied in cancer research. Alpinetin is a type of natural flavonoid primarily derived from Alpinia katsumadai Hayata, which exhibits anti‑bacterial and anti‑inflammatory properties. Therefore, it may possess anticancer potential and be employed therapeutically for different diseases. The aim of the present study was to investigate the anticancer effects of alpinetin on the SKOV3 ovarian cancer cell line. The effect of alpinetin treatment on SKOV3 cell proliferation, apoptosis, spheroid and colony formation were measured using Cell Counting kit‑8, cell apoptosis, 3D spheroid and colony formation assays, respectively...
August 22, 2018: Molecular Medicine Reports
Matt S Zinter, Kevin L Delucchi, Michele Y Kong, Benjamin E Orwoll, Aaron S Spicer, Michelle J Lim, Mustafa F Alkhouli, Anna E Ratiu, Anne V McKenzie, Patrick S McQuillen, Christopher C Dvorak, Carolyn S Calfee, Michael A Matthay, Anil Sapru
RATIONALE: Matrix metalloproteinases (MMPs) and their endogenous tissue inhibitors (TIMPs) may contribute to lung injury through extracellular matrix degradation and modulation of inflammation and fibrosis. OBJECTIVES: To test for an association between MMP pathway proteins and inflammation, endothelial dysfunction, and clinical outcomes. METHODS: We measured MMPs in plasma collected on ARDS day 1 from 235 children at 5 hospitals between 2008-2017...
August 16, 2018: American Journal of Respiratory and Critical Care Medicine
I Radziejewska, K Supruniuk, J Nazaruk, E Karna, B Popławska, A Bielawska, A Galicka
Rosmarinic acid (RA) is a natural phenylpropanoid with numerous pharmacological activities. Because of limited studies of the effects of RA action in gastric cancer cells we examined how 100 and 200 μM acid influences MMPs, TIMPs, collagen, MUC1 and specific sugar antigens in gastric adenocarcinoma CRL-1739 cells. We revealed inhibitory effect of RA on MMP-9 activity what was correlated with increased collagen type I expression, main ECM substrate degraded by MMPs. Tissue inhibitor of MMPs, TIMP-1 but not TIMP-2 was significantly decreased on the protein level and increased on mRNA level by RA action what can suggest TIMP-1 independent inhibitory action of an acid on MMP-9 activity...
August 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Odin Goovaerts, Pauline N M Mwinzi, Erick M O Muok, Ann Ceulemans, Robert Colebunders, Luc Kestens
BACKGROUND: Among the different faces of immune reconstitution inflammatory syndrome (IRIS) developing in HIV-patients, no clinical definition has been reported for Schistosomiasis-IRIS (Schisto-IRIS). Although Schisto-IRIS remains largely uninvestigated, matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) have previously been associated with S. mansoni infection and tuberculosis-IRIS. Here, we aimed to investigate the relevance of these markers in Schisto-IRIS...
August 2018: PLoS Neglected Tropical Diseases
Tram T Vuong, Sissel B Rønning, Tamer A E Ahmed, Kristiane Brathagen, Vibeke Høst, Maxwell T Hincke, Henri-Pierre Suso, Mona E Pedersen
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage to cure wounds, and is available in large quantities from egg industries. We have previously demonstrated that processed eggshell membrane powder (PEP), aiming to be used in a low cost wound healing product, possesses anti-inflammatory properties. In this study, we further investigated effects of PEP on MMP activities in vitro (a dermal fibroblast cell culture system) and in vivo (a mouse skin wound healing model)...
2018: PloS One
L B Kim, G S Russkih, A N Putyatina, O B Tsypysheva
Here we present the study of enzymes involved in the regulation of extracellular matrix metabolism. It was the first study of this kind in the European part of the Arctic zone of the Russian Federation (AZRF). The contents of matrix metalloproteinases (MMP-1, -2, -3, -9) and tissue inhibitors of matrix metalloproteinases (TIMP-1, -2, -4) were measured in blood plasma of 91 men in AZRF (67o N) and 14 men in Western Siberia. The contents of MMP-1, MMP-9, TIMP-1 and TIMP-4 in plasma of northerners were higher compared to those of the residents of Western Siberia (55o N)...
2018: Advances in Gerontology, Uspekhi Gerontologii
De-Bin Guo, Xiang-Qing Zhu, Qing-Qing Li, Gao-Mi-Yang Liu, Guang-Ping Ruan, Rong-Qing Pang, Yu-Hao Chen, Qiang Wang, Jin-Xiang Wang, Ju-Fen Liu, Qiang Chen, Xing-Hua Pan
Umbilical cord mesenchymal stem cells (UC-MSCs) exert strong immunomodulatory effects and can repair organs. However, their roles in radiation injury remain unclear. We show that in tree shrews with acute radiation injury, injected UC-MSCs significantly improved survival rates, reduced lung inflammation and apoptosis, prevented pulmonary fibrotic processes, recovered hematopoiesis, and increased blood counts. A protein microarray analysis showed that serum levels of the anti-inflammatory cytokines IL-10 and IL-13 and the growth factors BMP-5, BMP-7, HGF, insulin, NT-4, VEGFR3, and SCF were significantly higher, while those of the inflammatory cytokines IL-2, TIMP-2, TNF-α, IFN-γ, IL-1ra, and IL-8 and the fibrosis-related factors PDGF-BB, PDGF-AA, TGF-β1, IGFBP-2, and IGFBP-4 were significantly lower in UC-MSC-injected animals...
July 31, 2018: Cytotechnology
Konrad A Szychowski, Anna K Wójtowicz, Jan Gmiński
Degradation products of elastin, i.e. elastin-derived peptides (EDPs), are involved in various physiological and pathological processes. EDPs are detectable in cerebrospinal fluid in healthy people and in patients after ischemic stroke. However, to date, no studies concerning the role of EDP in the nervous system were conducted. Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) play important roles during the repair phases of cerebral ischemia, particularly during angiogenesis and reestablishment of cerebral blood flow...
July 30, 2018: Neurotoxicity Research
Cai-Xia Li, Li-Hua Cui, Yu-Zhen Zhuo, Jian-Gong Hu, Nai-Qiang Cui, Shu-Kun Zhang
AIMS: Autophagy is an intracellular metabolic process that degrades and recycles own constituents to maintain homeostasis and supply substrates. Disruption of collagen degradation is one of the pathogenesis of pancreatic fibrosis. In this study, we investigated the effects of inhibiting autophagy on the collagen degradation of PSCs. MAIN METHODS: Rats were injected dibutyltin dichloride (DBTC) to induce chronic pancreatitis (CP) model. The expression of LC3B was measured by western blotting...
September 1, 2018: Life Sciences
Xin Zhao, Yongcai Qi, Ruokun Yi, Kun-Young Park
Bamboo salt is generated by baking bamboo and sea salt and is used as a traditional food or medicine. The aim of this study was to investigate the anti-ageing skin effects of Korean bamboo salt and to compare the antioxidant, anti-ageing and anti-inflammatory effects of various salts, including purified salt, solar salt, bath solar salt, Masada solar salt, 1-time baked bamboo salt (1× bamboo salt), and 9-times baked bamboo salt (9× bamboo salt). Based on the content of mineral elements, pH, OH groups and redox potential amperometric analysis, the 9× bamboo salt showed the most antioxidant components and characteristics compared to the other salts...
July 24, 2018: International Journal of Biochemistry & Cell Biology
Νarjes Nasiri-Ansari, Georgios K Dimitriadis, Georgios Agrogiannis, Despoina Perrea, Ioannis D Kostakis, Gregory Kaltsas, Athanasios G Papavassiliou, Harpal S Randeva, Eva Kassi
BACKGROUND: Sodium glucose co-transporter2 inhibitors reduce the incidence of cardiovascular events in patients with type 2 diabetes mellitus based on the results of recent cardiovascular outcome studies. Herein, we investigated the effects of long-term treatment with canagliflozin on biochemical and immunohistochemical markers related to atherosclerosis and atherosclerosis development in the aorta of apolipoprotein E knockout (Apo-E(-/-) ) mice. METHODS: At the age of 5 weeks, mice were switched from normal to a high-fat diet...
July 26, 2018: Cardiovascular Diabetology
Sevil Korkmaz-Icöz, Paige Brlecic, Mihály Ruppert, Tamás Radovits, Matthias Karck, Gábor Szabó
OBJECTIVES: Hypertension can impair structure and function of blood vessels. Experimental data describing the reverse remodeling process after a mechanical pressure unloading therapy in the vasculature is limited. We studied the influence of pressure unloading on both the structural and functional alterations of the aorta in a hypertensive rat model. METHODS: Using isolated thoracic aortic rings in an in-vitro organ bath system, endothelium-dependent and endothelium-independent vasorelaxation were studied 6-weeks or 12-weeks after abdominal aortic banding (aortic banding-6-week or aortic banding-12-week), and 6-weeks after an aortic debanding procedure performed after the sixth experimental week of aortic banding (aortic banding + debanding-12-week)...
July 24, 2018: Journal of Hypertension
Maelíosa T C McCrudden, Katherine O'Donnell, Chris R Irwin, Fionnuala T Lundy
Mounting evidence suggests that the host defence peptide, LL-37, plays a role in both inflammation and in wound healing; however, the role of this peptide in the remodeling and maintenance of oral tissues is not yet fully understood. Fibroblasts are the most abundant cell type within the periodontal tissues, and gingival fibroblasts play an important role in maintaining and repairing the gingival tissues which are constantly exposed to external insults. In this study we examined the direct effects of LL-37 treatment on gingival fibroblasts and found that LL-37 significantly increased secretion of both interleukin 8 (IL-8) and IL-6 from these cells...
July 23, 2018: Vaccines
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