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Hussam Al-Humadi, Ahmed Alhumadi, Rafal Al-Saigh, Athina Strilakou, Andreas Lazaris, Maria Gazouli, Charis Liapi
Choline deprivation is a recognized experimental approach to non-alcoholic steatohepatitis, while thioacetamide- (TAA) induced liver fibrosis resembles alcoholic liver fibrogenesis. In order to elucidate the effect of TAA on liver extracellular matrix composition under choline deprivation due to choline-deficient diet (CDD) administration, we evaluated the transcriptional and immunohistochemical (IHC) pattern of major hepatic matrix metalloproteinases (namely, MMP-2, -9) and their tissue inhibitors (TIMP-1, -2) in adult male albino Wistar rats at 30, 60 and 90 days...
July 18, 2018: Clinical and Experimental Pharmacology & Physiology
Guoliang Zhang, Yunxiao Kang, Chenming Zhou, Rui Cui, Min Jia, Shen Hu, Xiaoming Ji, Jiayu Yuan, Huixian Cui, Geming Shi
Androgen plays a pivotal role in the progression of renal fibrosis. However, whether exogenous androgen treatment to aged male rats can improve the age-related renal fibrosis was not explored. In our study, the changes of morphological structure, renal fibrosis, ultrastructure and renal function, the expressions of extracellular matrix (ECM), matrix metalloproteinases (MMPs) and its tissue inhibitors of metalloproteinases (TIMPs), the expressions of tumor growth factor β1 (TGF-β1)/Smad signaling and oxidative stress parameters as well as nuclear factor erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE) signaling were tested in kidney of aged male Wistar rats after subcutaneous testosterone propionate (TP, 2 mg/kg/d, 84-day) injection...
July 16, 2018: Scientific Reports
Federico Carbone, Giorgia Bodini, Matteo Brunacci, Aldo Bonaventura, Alessandra Vecchiè, Luca Liberale, Mattia Crespi, Isabella Baldissarro, Franco Dallegri, Vincenzo Savarino, Fabrizio Montecucco, Edoardo G Giannini
BACKGROUND: Growing evidence indicates tissue inhibitors of matrix metalloproteinases (TIMPs) as potential players in inflammatory bowel disease (IBD), but, no prospective data are available in IBD remission/relapse. MATERIAL & METHODS: In this prospective pilot study, a cohort of IBD patients (n=32) was enrolled and treated with monoclonal anti-TNF-α antibodies. Patients were clinically followed up for a median period of 54 weeks. Serum circulating levels of C-reactive protein (CRP), TIMP-1 and -2, matrix metalloproteinase (MMP)-9 and -8, myeloperoxidase (MPO) and neutrophil elastase (NE) were assessed by ELISA at enrollment and at the end of the treatment...
July 16, 2018: European Journal of Clinical Investigation
Zongze Wang, Ying Li, Fengxin Zhou, Zhe Piao, Jian Hao
The purpose of the present study was to investigate the anticancer effects of β-elemene and paclitaxel for bone neoplasms. MTT assay, reverse transcription-quantitative polymerase chain reaction, western blotting, flow cytometry and immunostaining were used to analyze the combined effects of β-elemene and paclitaxel both in vitro and in vivo . The results demonstrated that combined treatment of β-elemene and paclitaxel (β-elemene-paclitaxel) significantly inhibited growth and aggressiveness of U-2OS cells compared with either β-elemene or paclitaxel treatment alone...
August 2018: Oncology Letters
Hong Li, Qiang Liu, Ningfu Wang, Yizhou Xu, Lan Kang, Yaqi Ren, Gangjie Zhu
BACKGROUND: In a previous study, a low level of miR-126-3p in endothelial progenitor cells (EPCs) was linked to the outcome of ischemic cardiomyopathy (ICM) patients. However, it remains unclear whether transplantation with miR-126-3p-overexpressing EPCs (MO-EPCs) can improve the cardiac function of ICM animal models.Methods and Results:miR-126-3p overexpression by lentiviral vector significantly increased migration and tube-like structures of EPCs from ICM patients. MO-EPCs or non-modified EPCs (NM-EPCs) were transplanted into nude rats with ICM induced by coronary artery ligation...
July 10, 2018: Circulation Journal: Official Journal of the Japanese Circulation Society
Chuan-Zhong Huang, Ai-Min Huang, Jing-Feng Liu, Bin Wang, Ke-Can Lin, Yun-Bin Ye
BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) is a major threat to human health. The condition carries a high risk of death; 45% of new cases occur in China. Surgical resection is the first choice for treatment of HCC, but 30.9% of patients experience recurrence within 6 months after the operation. To improve patient survival, we must determine how to reduce the probability of recurrence and metastasis and elucidate the underlying mechanism of disease. We therefore studied the effect of somatostatin octapeptide (octreotide) on the invasion and metastasis of HCC...
July 10, 2018: Cellular Physiology and Biochemistry
Lenka Fáber, Ivan Kováč, Petra Mitrengová, Martin Novotný, Lenka Varinská, Tomáš Vasilenko, Martin Kello, Matúš Čoma, Tomáš Kuruc, Klaudia Petrová, Ivana Miláčková, Anika Kuczmannová, Vlasta Peržeľová, Štefánia Mižáková, Erik Dosedla, František Sabol, Ján Luczy, Milan Nagy, Jaroslav Majerník, Martin Koščo, Pavel Mučaji, Peter Gál
Selective estrogen receptor modulators (SERMs) have been developed to achieve beneficial effects of estrogens while minimizing their side effects. In this context, we decided to evaluate the protective effect of genistein, a natural SERM, on skin flap viability in rats and in a series of in vitro experiments on endothelial cells (migration, proliferation, antioxidant properties, and gene expression profiling following genistein treatment). Our results showed that administration of genistein increased skin flap viability, but importantly, the difference is only significant when treatment is started 3 days prior the flap surgery...
July 4, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Ivan Göcze, Katharina Ehehalt, Florian Zeman, Paloma Riquelme, Karin Pfister, Bernhard M Graf, Thomas Bein, Edward K Geissler, Piotr Kasprzak, Hans J Schlitt, John A Kellum, James A Hutchinson, Elke Eggenhofer, Philipp Renner
BACKGROUND: Basic science data suggest that acute kidney injury (AKI) induced by ischemia-reperfusion injury (IRI) is an inflammatory process involving the adaptive immune response. Little is known about the T-cell contribution in the very early phase, so we investigated if tubular cellular stress expressed by elevated cell cycle biomarkers is associated with early changes in circulating T-cell subsets, applying a bedside-to-bench approach. METHODS: Our observational pilot study included 20 consecutive patients undergoing endovascular aortic repair for aortic aneurysms affecting the renal arteries, thereby requiring brief kidney hypoperfusion and reperfusion...
July 4, 2018: Critical Care: the Official Journal of the Critical Care Forum
Moritz Schanz, Christoph Wasser, Sebastian Allgaeuer, Severin Schricker, Juergen Dippon, Mark Dominik Alscher, Martin Kimmel
Background: Early detection and prevention of acute kidney injury (AKI) is important to reduce morbidity and mortality. Discovery of early-detection biomarkers has enabled early preventive approaches. There are no data on early biomarker-guided intervention with nephrological consultation in emergency departments (EDs). Methods: In this prospective randomized controlled intervention trial, patients at high risk for AKI were screened with urinary [TIMP-2]·[IGFBP7] in the ED of Robert-Bosch-Hospital (Stuttgart, Germany)...
June 28, 2018: Nephrology, Dialysis, Transplantation
Min Wen, Jin Hai Zheng, Jin Myung Choi, Jian Pei, Chun-Hao Li, Song-Yuan Li, In-Young Kim, Sa-Hoe Lim, Tae-Young Jung, Kyung-Sub Moon, Jung-Joon Min, Shin Jung
Glioma is one of the most devastating and refractory cancers. The main factors underlying therapeutic failure include extremely invasive characteristics and lack of effective methods for drug delivery. Attenuated Salmonella strains presented a high concentration of tumor targets in various types of cancer models, suggesting a role as potential vectors for drug delivery. In this study, we genetically engineered an attenuated strain of Salmonella as an anti-invasive vector for the targeted delivery and expression of tissue inhibitor of metalloproteinases 2 (TIMP-2) in an orthotopic nude mouse model of glioma...
June 26, 2018: Cancer Letters
Ling Wang, Meng-Ying Liu, Gang Yin, Na Li, Da-Bo Wang
Objective: To examine the effect of tissue inhibitor of metalloproteinase-2 (TIMP-2) on conjunctival filtering bleb scarring. Methods: A model of conjunctival filtering bleb was established whereby rats were injected with saline, blank adenoviral vector, or adenoviral vector carrying TIMP-2 into the bleb. Filtration bleb formation and matrix metalloproteinase-2 (MMP-2) expression were examined. Results: All operated eyes formed obvious elevated blebs on day 1...
2018: BioMed Research International
Zhou Chen, He Huang, Qi-Yu Chen
To investigate the curative effect of modified Buzhong Yiqi decoction combined with pelvic floor muscle exercise-biofeedback-electrostimulation on early postpartum pelvic floor dysfunction disease (PFD) and its effect on the expression of transforming growth factor (TGF-β1), matrix metalloprotein (MMP-2), and tissue inhibitor of metalloproteinase (TIMP-2). A total of 186 PFD patients admitted to our hospital from March 2014 to December 2016 were selected in the study and were randomly divided into test group ( n =93) and control group ( n =93)...
June 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Luke E Hodgson, Richard M Venn, Steve Short, Paul J Roderick, Duncan Hargreaves, Nicholas Selby, Lui G Forni
INTRODUCTION: Early recognition of patients developing acute kidney injury is of considerable interest, we report the first use of a combination of a clinical prediction rule with a biomarker in emergent adult medical patients to improve AKI recognition. METHODS: Single-centre prospective pilot study of medical admissions without AKI identified as high risk by a clinical prediction rule. Urine samples were obtained and tissue inhibitor of metalloproteinases-2 (TIMP-2) and insulin-like growth factor binding protein 7 (IGFBP7) - biomarkers associated with cell cycle arrest, were measured...
June 26, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Lei Liu, Yiping Li, Zhi Wang, Fengan Ding, Zhengyuan Cheng, Qing Xu, Yingying Cai, Manoj Hang Limbu, Yiqiong Yang, Bicheng Liu, Pingsheng Chen
Most patients with chronic kidney disease (CKD) present with proteinuria and extracellular matrix (ECM) deposition in the interstitium. Matrix metalloproteinase-2 (MMP-2) is important for maintaining ECM metabolism and it affects the formation and development of CKD. Autophagy has been reported to be protective against renal tubular injury, but the role of autophagy related to ECM metabolism is unclear. Rab7 is a shared molecule of endocytosis and autophagy. The aim of this study is to explore the role of autophagy in regulating MMP-2 activity and to determine whether Rab7 functions in regulating MMP-2 activity and injury in albumin-overloaded TECs...
June 19, 2018: Experimental Cell Research
Evelise Regina Polina, Raquel Rosa Candebat Vallejo Araújo, Renan Cesar Sbruzzi, Andréia Biolo, Luís Eduardo Rohde, Nadine Clausell, Kátia Gonçalves Dos Santos
Dysregulated expression of tissue inhibitors of matrix metalloproteinases (TIMPs) is associated with systolic dysfunction and worsening heart failure (HF). However, no study has assessed the relationship between TIMP polymorphisms and chronic HF. In this study, 300 HF outpatients with reduced left ventricular ejection fraction and 304 healthy blood donors were genotyped for the 372 T > C polymorphism (Phe124Phe; rs4898) in the TIMP-1 gene and the -418 G > C polymorphism (rs8179090) in the TIMP-2 gene to investigate whether these polymorphisms are associated with HF susceptibility and prognosis...
June 21, 2018: Scientific Reports
Boo-Yong Sim, Hak-Joo Choi, Min-Goo Kim, Dong-Gu Jeong, Don-Gil Lee, Jong-Min Yoon, Dae-Jung Kang, Soobong Park, Joong-Gu Ji, In-Hwan Joo, Dong-Hee Kim
Osteoarthritis is a disease that affects the articular cartilage and osseous tissue, and can be worsened by aging, overweight status, and post-traumatic arthritis. The present study aimed to evaluate the effect of ID-CBT5101 (tyndallized Clostridium butyricum ) on bone metabolism and the inflammatory response in a monosodium iodoacetate-induced rat model of osteoarthritis. ID-CBT5101 was administered orally at doses of 108 CFU/day or 1010 CFU/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 µL of 0...
May 11, 2018: Journal of Microbiology and Biotechnology
Y Ueda, A Inui, Y Mifune, R Sakata, T Muto, Y Harada, F Takase, T Kataoka, T Kokubu, R Kuroda
Objectives: The aim of this study was to investigate the effect of hyperglycaemia on oxidative stress markers and inflammatory and matrix gene expression within tendons of normal and diabetic rats and to give insights into the processes involved in tendinopathy. Methods: Using tenocytes from normal Sprague-Dawley rats, cultured both in control and high glucose conditions, reactive oxygen species (ROS) production, cell proliferation, messenger RNA (mRNA) expression of NADPH oxidase (NOX) 1 and 4, interleukin-6 (IL-6), matrix metalloproteinase (MMP)-2, tissue inhibitors of matrix metalloproteinase (TIMP)-1 and -2 and type I and III collagens were determined after 48 and 72 hours in vitro ...
May 2018: Bone & Joint Research
A Atici, S Emet, R Cakmak, G Yuruyen, A Alibeyoglu, M Akarsu, Y Arman, M Kose, E Ozgun, M Ozcan, O Altun, I Onur, T Tukek
OBJECTIVE: Type 1 cardiorenal syndrome (CRS) is an acute renal failure in patients with acute decompensated heart failure with an incidence of 24% to 45%. The aim of our study was to investigate the significance of new renal biomarkers to predict type 1 CRS. PATIENTS AND METHODS: The study included 111 patients with acute decompensated heart failure diagnosed at the Istanbul Medical Faculty Emergency Department between 2014 and 2016, and 24 healthy volunteers. All urine samples were stored at -80°C after centrifugation...
June 2018: European Review for Medical and Pharmacological Sciences
Malgorzata Kobusiak-Prokopowicz, Justyna Krzysztofik, Konrad Kaaz, Beata Jolda-Mydlowska, Andrzej Mysiak
The aim of the study was to assess MMP-2 (matrix metalloproteinase-2) and TIMP-2 (tissue inhibitor of metalloproteinase-2) serum levels in patients with diverse types of heart failure (HF) and chronic kidney disease (CKD). 101 patients with chronic HF were enrolled. Each patient has assessed the serum levels of MMP-2, TIMP-2, and NT-proBNP. Patients were initially classified into 2 groups based on their LVEF. 43 patients were classified into the HFREF group (HF with Reduced Ejection Fraction) and 58 characterized as HFPEF (HF with Preserved Ejection Fraction)...
2018: Open Medicine (Warsaw, Poland)
Erin F Barreto, Andrew D Rule, Stacy A Voils, Sandra L Kane-Gill
Over the last decade, the discovery of novel renal biomarkers and research on their use to improve medication effectiveness and safety has expanded considerably. Pharmacists are uniquely positioned to leverage this new technology for renal assessment to improve medication dosing and monitoring. Serum cystatin C is a relatively new, inexpensive, functional renal biomarker that responds more quickly to changing renal function than creatinine and is not meaningfully affected by age, sex, skeletal muscle mass, dietary intake, or deconditioning...
June 8, 2018: Pharmacotherapy
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