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Konstantinos Lathouras, Srdjan Saso, Menelaos Tzafetas, Kallirroi Kalinderi, Styliani Fidani, Vassiliki Zournatzi, Maria Kyrgiou, Christina Fotopoulou, Sadaf Ghaem-Maghami, Ioannis Tzafetas
Aim: Extracellular matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitors of metalloproteinases [TIMPs]) are involved in the breakdown of fetal membranes before delivery. Our aim was to investigate the occurrence of any polymorphism on genes coding for MMPs 1-3 and TIMP 2 in preterm laboring patients as a potential source of this phenomenon. This question has not been studied before. Methodology & results: A prospective population study was performed in a Greek university hospital...
October 2018: Future Science OA
Gamze Tuna, Görsev G Yener, Gülgün Oktay, G Hüray İşlekel, F Güldal Kİrkalİ
Matrix metalloproteinases (MMPs) are substantial regulators of learning and memory and might be involved in neurodegeneration. It is known that MMPs are involved in pathogenesis of Alzheimer's disease (AD) and are particularly involved in the amyloid-β processing pathway. However, information on circulating levels of these proteins and their tissue inhibitors (TIMPs) in AD and other neurodegenerative dementia (ND) diseases such as dementia with Lewy bodies (DLB) and frontotemporal dementia (FTD) is not clear...
November 5, 2018: Journal of Alzheimer's Disease: JAD
Tyler Lopez, Aaron Ramirez, Chris Benitez, Zahid Mustafa, Henry Pham, Ramon Sanchez, Xin Ge
Background : Proteases are one of the largest pharmaceutical targets for drug developments. Their dysregulations result in a wide variety of diseases. Because proteolytic networks usually consist of protease family members that share high structural and catalytic homology, distinguishing them using small molecule inhibitors is often challenging. To achieve specific inhibition, this study described a novel approach for the generation of protease inhibitory antibodies. As a proof of concept, we aimed to convert a matrix metalloproteinase (MMP)-14 specific inhibitor to MMP-9 specific inhibitory antibodies with high selectivity...
September 2018: Antibody therapeutics
Emmanuelle Rouve, Karim Lakhal, Charlotte Salmon Gandonnière, Youenn Jouan, Laetitia Bodet-Contentin, Stephan Ehrmann
BACKGROUND: Iodinated contrast media may contribute to acute kidney injury. However, several recent works suggest that this toxicity is minimal in the clinical setting. Recently, urinary G1 cell-cycle arrest proteins tissue inhibitor of metalloproteinase 2 (TIMP-2) and insulin like growth factor binding protein 7 (IGFBP-7) were identified as highly sensitive and specific biomarkers for early detection of kidney aggression. The impact of contrast administration on those biomarkers has not been specifically evaluated but could provide clues about the toxicity of contrast media...
November 6, 2018: BMC Nephrology
A G A Viana, A M A Martins, A H Pontes, W Fontes, M S Castro, C A O Ricart, M V Sousa, A Kaya, E Topper, E Memili, A A Moura
Male fertility is the ability of sperm to fertilize the egg and sustain embryo development. Several factors determine the fertilizing capacity of mammalian sperm, including those intrinsic to sperm and components of the seminal plasma. The present study analyzed the seminal fluid proteome of Bos taurus and potential associations between proteins and fertility scores. Mass spectrometry coupled with nano HPLC allowed the identification of 1,159 proteins in the dairy bull seminal plasma. There were 50 and 29 seminal proteins more abundant in high (HF) low fertility (LF) bulls, respectively...
November 5, 2018: Scientific Reports
Elizabeth F Johnston, Todd E Gillis
The collagen content of the rainbow trout heart increases in response to cold acclimation, and decreases with warm acclimation. This ability to remodel the myocardial extracellular matrix (ECM) makes these fish useful models to study the cellular pathways involved in collagen regulation in the vertebrate heart. Remodeling of the ECM in the mammalian heart is regulated, in part, by myofibroblasts which arise from pre-existing fibroblasts in response to transforming growth factor-beta 1 (TGF-β1). We have previously demonstrated that treatment of cultured rainbow trout cardiac fibroblasts with human TGF-β1 causes an increase in collagen production...
November 5, 2018: Journal of Experimental Biology
Jared J Cummings, Andrew D Shaw, Jing Shi, Marcos G Lopez, Jason B O'Neal, Frederic T Billings
OBJECTIVE: Tissue inhibitor of metalloproteinases 2 (TIMP-2) and insulin-like growth factor-binding protein 7 (IGFBP7) are postoperative urinary biomarkers of renal stress and acute kidney injury (AKI). We conducted this study to test the hypothesis that intraoperative concentrations of urinary [TIMP-2]·[IGFBP7] are associated with postoperative AKI. METHODS: We measured urinary [TIMP-2]·[IGFBP7] at 8 perioperative timepoints in 400 patients who participated in a randomized controlled trial of atorvastatin for AKI in cardiac surgery...
September 26, 2018: Journal of Thoracic and Cardiovascular Surgery
Lin Zhang, Chang Chen, Jiaxin Duanmu, Yan Wu, Jinhua Tao, Aihua Yang, Xiaoqin Yin, Biao Xiong, Jingya Gu, Chunling Li, Zhaoguo Liu
The aim of this study was to evaluate the pharmacological effects of CPT on CT26 colon cancer cells in vivo and in vitro, and to reveal the potential mechanism. CPT suppressed the proliferation and growth of CT26 colon cancer in vitro and in vivo. CPT inhibited the invasion of CT26 cells in vitro, and decreased the protein expressions of matrix metalloproteinase-2 (MMP-2) and MMP-9 but increased those of tissue inhibitor of metallopeptidase-1 (TIMP-1) and TIMP-2 in vitro and in vivo. It also inhibited tumor cell-induced angiogenesis of endothelial cells in vitro and rat aortic ring angiogenesis ex vivo, and possibly by suppressing angiogenesis-associated factors...
October 30, 2018: International Immunopharmacology
Yujiao Tang, Meiqi Fan, Young-Jin Choi, Yonghai Yu, Gang Yao, Yongyan Deng, Sang-Ho Moon, Eun-Kyung Kim
The present study determines whether antler extract (AE) possesses inhibitory effects in a prostate cancer (PC) xenograft model and explores the underlying mechanism. After therapeutic intervention for two weeks, AE significantly inhibited prostate cancer xenograft tumor growth by 65.08%, and prostate-specific antigen (PSA) and serum dihydrotestosterone (DHT) levels. However, AE increased the serum testosterone level compared to the vehicle control group. Furthermore, our investigation of the inhibitory effects on angiogenesis and epithelial-to-mesenchymal transition (EMT)-related genes revealed that AE downregulated matrix metalloproteinase 2 (MMP)-2, (MMP)-9, vascular endothelial growth factor (VEGF), zinc finger protein (SNAIL1), twist-related protein 1 (TWIST1), and zinc-finger E-box-binding homeobox 1 (ZEB1) in vivo...
October 31, 2018: Bioscience, Biotechnology, and Biochemistry
Lai Jiang, Jun Qian, Yongbo Yang, Yongtian Fan
BACKGROUND Colon cancer is one of the most common cancers in the world. We performed the present study to determine the molecular mechanism of MON1B in colon cancer cells. MATERIAL AND METHODS Colon cancer tissues and adjacent normal tissues were collected from 34 colon cancer patients. MON1B-silenced LoVo colon cancer cells were constructed. RT-qPCR and Western blot analysis were used to detect mRNA and protein levels, respectively, of colon cancer tissues and cells. Cell counting kit-8 (CCK-8), wound healing, and Transwell assays were used to detect viability, migration, and invasion, respectively, of colon cancer cells...
October 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Cristina Sánchez-Quesada, Alicia López-Biedma, Estefania Toledo, José J Gaforio
Anti-inflammatory effects of virgin olive oil (VOO) have been described recently, along with its wound healing effect. One of the main minor compounds found in VOO is squalene (SQ), which also possesses preventive effects against skin damage and anti-inflammatory properties. The inflammatory response is involved in wound healing and manages the whole process by macrophages, among others, as the main innate cells with a critical role in the promotion and resolution of inflammation for tissue repair. Because of that, this work is claimed to describe the role that squalene exerts in the immunomodulation of M1 proinflammatory macrophages, which are the first cells implicate in recent injuries...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Sapir Karli, Juan Alfredo Ayala-Haedo, William J Feuer, Maria Fernandez, Sander Dubovy, Sara T Wester
Purpose: To characterize the effect of prostaglandin analogs (PAs) on tissue specific expression of matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in levator aponeurosis resections (LAR) and conjunctiva-Muller muscle resections (CMMR). Methods: Specimens from LAR and CMMR of PA users and non-users were analyzed for tissue specific expression of MMP-3, MMP-7, MMP-9 and TIMP-2 using immunohistochemistry. PA use, marginal reflex distances, levator function and palpebral fissure were documented through chart review...
2018: Clinical Ophthalmology
Julia Neuhaus, Frederic Bauer, Christina Fitzner, Ralf-Dieter Hilgers, Felix Seibert, Nina Babel, Adrian Doevelaar, Frank Eitner, Jürgen Floege, Thomas Rauen, Timm H Westhoff
BACKGROUND/AIMS: The addition of immunosuppression to supportive care reduces proteinuria in a subset of patients with IgA nephropathy (IgAN) but is associated with an increased rate of adverse events. The present work investigates whether urinary biomarkers are able to identify subjects who benefit from immunosuppression and to predict the progression of disease in a sub-cohort of the STOP-IgAN trial. METHODS: Urinary neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), calprotectin, and the product of tissue inhibitor of metalloproteinase-2 and insulin-like growth factor-binding protein 7 (TIMP2•IGFBP7) were measured in all available urine samples obtained at the time point of enrollment in the STOP-IgAN trial (n=113)...
2018: Kidney & Blood Pressure Research
X Zhou, X Zang, Y Guan, T Tolbert, T C Zhao, G Bayliss, S Zhuang
Despite the established oncogenic and profibrotic functions of enhancer of zeste homolog 2 (EZH2), a methyltransferase that induces histone H3 lysine 27 trimethylation (H3K27me3), its role in acute kidney injury (AKI) remains unclear. In this study, we demonstrated that EZH2 and H3K27me3 were upregulated in the murine kidney with AKI induced by either ischemia-reperfusion (I/R) or folic acid (FA). Pharmacologic inhibition of EZH2 with 3-deazaneplanocin A (3-DZNeP) prevented tubular injury in both models as demonstrated by reduced renal dysfunction, diminished neutrophil gelatinase-associated lipocalin expression and decreased renal tubular cell death...
October 19, 2018: Cell Death & Disease
Luping Liu, Li Sun, Jing Zheng, Yanchun Wang
BACKGROUND The improper invasion of trophoblast cells (TC) can cause various diseases. BRCT-repeat inhibitor of hTERT expression (BRIT1) is involved in the invasion of tumors. Here, we analyzed the effects of BRIT1 on the invasion of TC. MATERIAL AND METHODS The expression of BRIT1 in JEG-3, B6Tert, and HTR8/SVneo cells was evaluated by transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting. The viability, invasion, and migration of HTR8/SVneo cells were measured using cell counting kit-8 (CCK-8) and Transwell assays...
October 19, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Jun Shen, Cao Yin, Xiao Jiang, Xuan Wang, Shujuan Yang, Guangbao Song
BACKGROUNDS: To investigate alterations in histone modification and histone deacetylases (HDACs) in patients with oral lichen planus (OLP), and to evaluate correlations with inflammatory cytokine production. METHODS: Global histone H3/H4 acetylation and HDAC activity in CD4+ T cells from 23 patients with OLP and 10 healthy control subjects were examined using spectrophotometry. The mRNA levels of 8 members of 4 classes of HDAC genes were measured by real-time quantitative polymerase chain reaction...
October 17, 2018: Journal of Oral Pathology & Medicine
Sonia Aloui, Wiem Zidi, Sana Ouali, Imen Guizani, Sameh Hadj-Taieb, Mohamed Sami Mourali, Moncef Feki, Monia Allal-Elasmi
Calcific mitral valve stenosis (MVS) is a common disease characterized by extensive remodeling of the extracellular matrix via matrix metalloproteinases (MMPs). The mechanism of calcification due to extensive matrix remodeling remains unclear. In this study, we investigated the relationship between MMP-3, tissue inhibitors of metalloproteinases (TIMPs) as well as pro-inflammatory cytokines and the phenomenon of calcification in MVS. 212 patients having rheumatic mitral stenosis (RMS) and 155 healthy control subjects were recruited in the Cardiology Department of La Rabta Hospital University...
October 9, 2018: Molecular Biology Reports
Jonna R Bank, Renée Ruhaak, Darius Soonawala, Oleg Mayboroda, Fred P Romijn, Cees van Kooten, Christa M Cobbaert, Johan W de Fijter
BACKGROUND: Urinary tissue inhibitor of metalloproteinases-2 (TIMP-2) and insulin-like growth factor binding protein-7 (IGFBP7) have been validated as biomarkers for acute kidney injury. We investigated the performance of both markers in predicting the occurrence and duration of functionally defined delayed graft function (fDGF) in donation after circulatory death (DCD) kidney transplant recipients. METHODS: Urine samples of 74 DCD recipients were analyzed. TIMP-2 and IGFBP7 were measured with ELISA on postoperative day 1 to 7, day 10, week 6 and month 6 and values were corrected for osmolality (mOsm)...
October 8, 2018: Transplantation
Sheng-Teng Huang, Cheng-Chieh Chang, Jong-Hwei S Pang, Hung-Sen Huang, Shen-Chieh Chou, Ming-Ching Kao, Huey-Ling You
Background and Purpose: Drynaria fortunei J. Sm ( D. fortunei ), known as Gu-Sui-Bu, is used in traditional Chinese medicine to treat common injuries, including bone fractures and bruising. The specific functional mechanisms of the angiogenic and endothelial cell migration properties of D. fortunei are currently unclear. Thus, the purpose of this study is to validate the potential angiogenic and cellular migration properties and related mechanisms by D. fortunei both in vivo and in vitro . Experimental Approach: The present study investigates, both in vivo and in vitro , the wound healing effects of D...
2018: Frontiers in Pharmacology
Katarzyna Oszajca, Maciej Szemraj, Janusz Szemraj, Piotr Jurowski
BACKGROUND: Age-related macular degeneration is a progressive eye disease affecting the macula and causing acute visual loss particularly in elder people. The aim of the study was an attempt to discern an influence of expression levels and functional genetic polymorphisms of selected genes related to the extracellular matrix turnover or neovascularization on age-related macular degeneration occurrence and progression. METHODS: We conducted a case-control study of 200 polish patients with recognized age-related macular degeneration (dry and wet) and compared the results with those obtained from matched 100 healthy control subjects...
October 5, 2018: Ophthalmic Genetics
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