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https://www.readbyqxmd.com/read/27912878/selecting-optimum-protein-nano-carriers-for-natural-polyphenols-using-chemoinformatics-tools
#1
AbdelKader A Metwally, Sherweit H El-Ahmady, Rania M Hathout
BACKGROUND: The normal fate of any natural product with a therapeutic potential is to be formulated into an effective medicine. However, the conventional methods of selecting the suitable formulations or carriers based on the formulator experiences, trials and errors as well as materials availability do not usually yield the optimal results. HYPOTHESIS: We hypothesize the possibility of the virtual optimum selection of a protein carrier for two polyphenolic compounds widely investigated for their chemopreventive effects; resveratrol and curcumin using a combination of some chemoinformatics tools...
December 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27910542/design-and-first-plasma-measurements-of-the-iter-ece-prototype-radiometer
#2
M E Austin, M W Brookman, W L Rowan, S Danani, E W Bryerton, P Dougherty
On ITER, second harmonic optically thick electron cyclotron emission (ECE) in the range of 220-340 GHz will supply the electron temperature (Te). To investigate the requirements and capabilities prescribed for the ITER system, a prototype radiometer covering this frequency range has been developed by Virginia Diodes, Inc. The first plasma measurements with this instrument have been carried out on the DIII-D tokamak, with lab bench tests and measurements of third through fifth harmonic ECE from high Te plasmas...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27908627/validation-of-a-two-generational-reproduction-test-in-daphnia-magna-an-interlaboratory-exercise
#3
Carlos Barata, Bruno Campos, Claudia Rivetti, Gerald A LeBlanc, Stephanie Eytcheson, Stephanie McKnight, Marysia Tobor-Kaplon, Selinda de Vries Buitenweg, Suhyon Choi, Jinhee Choi, Elena I Sarapultseva, Marie-Agnès Coutellec, Maïra Coke, Pascal Pandard, Arnaud Chaumot, Hervé Quéau, Nicolas Delorme, Olivier Geffard, Fernando Martínez-Jerónimo, Haruna Watanabe, Norihisa Tatarazako, Isabel Lopes, João L T Pestana, Amadeu M V M Soares, Cecilia Manuela Pereira, Karel De Schamphelaere
Effects observed within one generation disregard potential detrimental effects that may appear across generations. Previously we have developed a two generation Daphnia magna reproduction test using the OECD TG 211 protocol with a few amendments, including initiating the second generation with third brood neonates produced from first generation individuals. Here we showed the results of an inter-laboratory calibration exercise among 12 partners that aimed to test the robustness and consistency of a two generation Daphnia magna reproduction test...
November 28, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27908280/development-of-a-fast-and-easy-method-for-escherichia-coli-genome-editing-with-crispr-cas9
#4
Dongdong Zhao, Shenli Yuan, Bin Xiong, Hongnian Sun, Lijun Ye, Jing Li, Xueli Zhang, Changhao Bi
BACKGROUND: Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS: In this research, the goal of development of a fast and easy method for Escherichia coli genome editing with high efficiency is pursued. For this purpose, we designed modular plasmid assembly strategy, compared effects of different length of homologous arms for recombination, and tested different sets of recombinases...
December 1, 2016: Microbial Cell Factories
https://www.readbyqxmd.com/read/27904520/a-diagnostic-one-step-real-time-reverse-transcription-polymerase-chain-reaction-method-for-accurate-detection-of-influenza-virus-type-a
#5
Mohammad Amin Behzadi, Mazyar Ziyaeyan, Abdolvahab Alborzi
INTRODUCTION: Influenza A is known as a public health concern worldwide. In this study, a novel one-step real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was designed and optimized for the detection of influenza A viruses. MATERIAL AND METHODS: The primers and probe were designed based on the analysis of 90 matrix nucleotide sequence data of influenza type A subtypes from the GenBank database of the National Center for Biotechnology Information (NCBI)...
December 1, 2016: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/27903258/catwalk-gait-analysis-in-a-rat-model-of-multiple-sclerosis
#6
Sabine Herold, Prateek Kumar, Klaus Jung, Irina Graf, Henrike Menkhoff, Xenia Schulz, Mathias Bähr, Katharina Hein
BACKGROUND: Myelin oligodendrocyte glycoprotein (MOG)-induced experimental autoimmune encephalomyelitis (EAE) is a widely used animal model for multiple sclerosis. The characteristic feature of the MOG-EAE model in Brown Norway rats is consistent involvement of the spinal cord resulting in limb paresis. The aim of the study was to investigate whether early subclinical gait abnormalities are present in this animal model and can be detected by CatWalk XT, a fully automated gait analysis system...
November 30, 2016: BMC Neuroscience
https://www.readbyqxmd.com/read/27902711/eeg-to-primary-rewards-predictive-utility-and-malleability-by-brain-stimulation
#7
Nicole Prause, Greg J Siegle, Choi Deblieck, Allan Wu, Marco Iacoboni
Theta burst stimulation (TBS) is thought to affect reward processing mechanisms, which may increase and decrease reward sensitivity. To test the ability of TBS to modulate response to strong primary rewards, participants hypersensitive to primary rewards were recruited. Twenty men and women with at least two opposite-sex, sexual partners in the last year received two forms of TBS. Stimulations were randomized to avoid order effects and separated by 2 hours to reduce carryover. The two TBS forms have been demonstrated to inhibit (continuous) or excite (intermittent) the left dorsolateral prefrontal cortex using different pulse patterns, which links to brain areas associated with reward conditioning...
2016: PloS One
https://www.readbyqxmd.com/read/27900494/semi-industrial-scale-30%C3%A2-m-3-fed-batch-fermentation-for-the-production-of-d-lactate-by-escherichia-coli-strain-hbut-d15
#8
Xiangmin Fu, Yongze Wang, Jinhua Wang, Erin Garza, Ryan Manow, Shengde Zhou
D(-)-lactic acid is needed for manufacturing of stereo-complex poly-lactic acid polymer. Large scale D-lactic acid fermentation, however, has yet to be demonstrated. A genetically engineered Escherichia coli strain, HBUT-D, was adaptively evolved in a 15% calcium lactate medium for improved lactate tolerance. The resulting strain, HBUT-D15, was tested at a lab scale (7 L) by fed-batch fermentation with up to 200 g L(-1) of glucose, producing 184-191 g L(-1) of D-lactic acid, with a volumetric productivity of 4...
November 29, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27900260/gut-microbiota-and-glucometabolic-alterations-in-response-to-recurrent-partial-sleep-deprivation-in-normal-weight-young-individuals
#9
Christian Benedict, Heike Vogel, Wenke Jonas, Anni Woting, Michael Blaut, Annette Schürmann, Jonathan Cedernaes
OBJECTIVE: Changes to the microbial community in the human gut have been proposed to promote metabolic disturbances that also occur after short periods of sleep loss (including insulin resistance). However, whether sleep loss affects the gut microbiota remains unknown. METHODS: In a randomized within-subject crossover study utilizing a standardized in-lab protocol (with fixed meal times and exercise schedules), we studied nine normal-weight men at two occasions: after two nights of partial sleep deprivation (PSD; sleep opportunity 02:45-07:00 h), and after two nights of normal sleep (NS; sleep opportunity 22:30-07:00 h)...
December 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27897004/identifying-genetic-associations-with-variability-in-metabolic-health-and-blood-count-laboratory-values-diving-into-the-quantitative-traits-by-leveraging-longitudinal-data-from-an-ehr
#10
Shefali S Verma, Anastasia M Lucas, Daniel R Lavage, Joseph B Leader, Raghu Metpally, Sarathbabu Krishnamurthy, Frederick Dewey, Ingrid Borecki, Alexander Lopez, John Overton, John Penn, Jeffrey Reid, Sarah A Pendergrass, Gerda Breitwieser, Marylyn D Ritchie
A wide range of patient health data is recorded in Electronic Health Records (EHR). This data includes diagnosis, surgical procedures, clinical laboratory measurements, and medication information. Together this information reflects the patient's medical history. Many studies have efficiently used this data from the EHR to find associations that are clinically relevant, either by utilizing International Classification of Diseases, version 9 (ICD-9) codes or laboratory measurements, or by designing phenotype algorithms to extract case and control status with accuracy from the EHR...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27896989/opening-the-door-to-the-large-scale-use-of-clinical-lab-measures-for-association-testing-exploring-different-methods-for-defining-phenotypes
#11
Christopher R Bauer, Daniel Lavage, John Snyder, Joseph Leader, J Matthew Mahoney, Sarah A Pendergrass
The past decade has seen exponential growth in the numbers of sequenced and genotyped individuals and a corresponding increase in our ability of collect and catalogue phenotypic data for use in the clinic. We now face the challenge of integrating these diverse data in new ways new that can provide useful diagnostics and precise medical interventions for individual patients. One of the first steps in this process is to accurately map the phenotypic consequences of the genetic variation in human populations. The most common approach for this is the genome wide association study (GWAS)...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27896972/data-sharing-and-reproducible-clinical-genetic-testing-successes-and-challenges
#12
Shan Yang, Melissa Cline, Can Zhang, Benedict Paten, Stephen E Lincoln
Open sharing of clinical genetic data promises to both monitor and eventually improve the reproducibility of variant interpretation among clinical testing laboratories. A significant public data resource has been developed by the NIH ClinVar initiative, which includes submissions from hundreds of laboratories and clinics worldwide. We analyzed a subset of ClinVar data focused on specific clinical areas and we find high reproducibility (>90% concordance) among labs, although challenges for the community are clearly identified in this dataset...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27896755/the-application-of-multiplex-biomarker-techniques-for-improved-stratification-and-treatment-of-schizophrenia-patients
#13
Johann Steiner, Paul C Guest, Hassan Rahmoune, Daniel Martins-de-Souza
In the case of major psychiatric disorders such as schizophrenia, shortcomings in the conversion of scientific discoveries into newer and safer treatment options has led to a loss of confidence and precipitated a crisis for large pharmaceutical companies. This chapter describes how incorporation of multiplex biomarker approaches into the clinical pipeline can lead to better patient characterization, delivery of novel treatment approaches and help to renew efforts in this important area. The development of specific biomarker test panels for disease prediction should facilitate early intervention strategies, which may help to slow disease development or progression...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27896639/seom-clinical-guideline-for-bone-metastases-from-solid-tumours-2016
#14
C Grávalos, C Rodríguez, A Sabino, M Á Seguí, J A Virizuela, A Carmona, J Cassinello, D Isla, C Jara, M Martín
Bone metastases are common in many advanced solid tumours, being breast, prostate, thyroid, lung, and renal cancer the most prevalent. Bone metastases can produce skeletal-related events (SREs), defined as pathological fracture, spinal cord compression, need of bone irradiation or need of bone surgery, and hypercalcaemia. Patients with bone metastases experience pain, functional impairment and have a negative impact on their quality of life. Several imaging techniques are available for diagnosis of this disease...
November 28, 2016: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/27896588/electrosprayed-myocet-like-liposomes-an-alternative-to-traditional-liposome-production
#15
Michael A Collier, Eric M Bachelder, Kristy M Ainslie
PURPOSE: Although doxorubicin (DXR) has been on the market for many years as an anti-cancer drug, a number of serious dose-limiting toxicities hinder its widespread use. To reduce the known toxicities of soluble DXR, various liposomes have been designed including Doxil, Caelyx, and Myocet. Myocet, a non-PEGylated liposomal formulation containing DXR, was found to reduce the toxicities associated with soluble DXR and has been used in Europe and Canada (but not the US) as a first line therapy...
November 28, 2016: Pharmaceutical Research
https://www.readbyqxmd.com/read/27892440/urinary-tract-infection-in-asymptomatic-newborns-with-prolonged-unconjugated-hyperbilirubunemia-a-hospital-based-observational-study-from-western-region-of-nepal
#16
T Malla, B Sathian, K Karmacharya Malla, S Adhikari
Background Urine culture is usually not a part of work-up for neonatal unconjugated hyperbilirubinemia; hence its prevalence remains unknown. Objective This study was done to determine the incidence of urinary tract infection (UTI) in asymptomatic newborns with prolonged unconjugated hyperbilirubinemia and to evaluate which other laboratory parameters are associated with UTIs. Method A prospective observational study where jaundiced newborns otherwise clinically well, were evaluated for UTI. The study was carried out in neonatal intensive care unit of Manipal Teaching Hospital, Pokhara from June 2012 -April 2013...
January 2016: Kathmandu University Medical Journal (KUMJ)
https://www.readbyqxmd.com/read/27890457/the-role-of-whole-genome-sequencing-wgs-in-antimicrobial-susceptibility-testing-of-bacteria-report-from-the-eucast-subcommittee
#17
REVIEW
Matthew J Ellington, Oskar Ekelund, Frank M Aarestrup, Rafael Canton, Michel Doumith, Christian Giske, Hajo Grundman, Henrik Hasman, Matthew Holden, Katie L Hopkins, Jon Iredell, Gunnar Kahlmeter, Claudio U Köser, Alasdair MacGowan, Dik Mevius, Mike Mulvey, Thierry Naas, Tim Peto, Jean-Marc Rolain, Ørjan Samuelsen, Neil Woodford
Whole genome sequencing (WGS) offers the potential to predict antimicrobial susceptibility from a single assay. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) established a subcommittee to review the current development status of WGS for bacterial antimicrobial susceptibility testing (AST). The published evidence for using WGS as a tool to infer antimicrobial susceptibility accurately is currently either poor or non-existent and the evidence / knowledge base requires significant expansion...
November 23, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27890161/primary-or-secondary-tasks-dual-task-interference-between-cyclist-hazard-perception-and-cadence-control-using-cross-modal-sensory-aids-with-rider-assistance-bike-computers
#18
Chao-Yang Yang, Cheng-Tse Wu
This research investigated the risks involved in bicycle riding while using various sensory modalities to deliver training information. To understand the risks associated with using bike computers, this study evaluated hazard perception performance through lab-based simulations of authentic riding conditions. Analysing hazard sensitivity (d') of signal detection theory, the rider's response time, and eye glances provided insights into the risks of using bike computers. In this study, 30 participants were tested with eight hazard perception tasks while they maintained a cadence of 60 ± 5 RPM and used bike computers with different sensory displays, namely visual, auditory, and tactile feedback signals...
March 2017: Applied Ergonomics
https://www.readbyqxmd.com/read/27889878/same-day-identification-and-antibiotic-susceptibility-testing-on-positive-blood-cultures-a-simple-and-inexpensive-procedure
#19
K Maelegheer, E Nulens
Fast diagnostic tools are becoming a hot topic in microbiology, especially in the case of septic patients. Therefore, we attempted to develop a fast, inexpensive, accurate and easy method to identify bacteria and perform an antibiotic susceptibility test directly on positive blood cultures that could be used in a routine laboratory. A procedure based on centrifugation and washing steps was performed on 110 non-duplicated (including nine seeded) positive blood culture bottles. Direct identification (DID) and antimicrobial susceptibility testing (AST) was conducted on the pellet with the MALDI Biotyper and Phoenix, respectively...
November 26, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27888958/improving-metabolic-syndrome-screening-on-patients-on-second-generation-antipsychotic-medication
#20
Esther Kioko, Kimberly Williams, Beth Newhouse
AIM: This quality improvement project aims at stressing the importance of screening for metabolic syndrome (MS) on patients with serious mental illness (SMI) managed with second generation antipsychotic (SGA) medication. METHOD: One hundred charts of patients who were on SGA (n=100) were randomly selected from more than 1000 charts for the purpose of this project with (n=50) charts for pre-intervention and (n=50) charts for post intervention. A chi-square test of independence was calculated comparing the frequency of labs and vital done in pre-intervention and post-intervention period...
December 2016: Archives of Psychiatric Nursing
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