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ovarian suppression

Siwei Liu, Bingyu Zou, Tian Tian, Xiaohui Luo, Banyun Mao, Xun Zhang, Huajiang Lei
To determine how the lncRNA FER1L4 in ovarian cancer cells influences paclitaxel (PTX) resistance, we examined the expression level of FER1L4 in human ovarian epithelial cell lines IOSE80 and HOSEpiC and human ovarian cancer cell lines OVCAR-3, Caov-3, and SKOV3 through RNA isolation and quantitative polymerase chain reaction (qRT-PCR). SKOV3 cell lines were treated with PTX. The cell survival rate and apoptosis rate of SKOV3 and SKOV3-PR at different PTX dose levels were evaluated. Next, qRT-PCR was performed to detect the expression of FER1L4 in SKOV3 and SKOV3-PR cell lines...
November 15, 2018: Journal of Cellular Biochemistry
Cai-Hong Zhang, Ye Wang, Qian-Qian Sun, Lei-Lei Xia, Jing-Jing Hu, Kai Cheng, Xia Wang, Xin-Xin Fu, Hang Gu
Copper nanoparticles (Cu-NPs) and other inorganic nanomaterials have caused increasing concern owing to be widely used. Early studies have reported that they can result in injuries to the kidney, liver and spleen of mice; cause embryonic damage; and inhibit the reproductive capacity of red worms. However, few studies have reported the toxicity of Cu-NPs on the reproductive systems of mammals. In the present work, we explored the cytotoxicity of Cu-NPs in human extravillous trophoblast cells and in the reproductive organs of mice...
2018: International Journal of Biological Sciences
Mrázová Veronika, Golais František, Búda
Seroepidemiological studies suggest that human herpes simplex virus type 1 (HSV-1) and 2 (HSV-2) are linked with several types of cancer; however, they do not appear to play a direct role and are considered to be cofactors. The abilities of HSV-1 and -2 to transform cells in vitro can be demonstrated by suppressing their lytic ability via irradiation with a specific dose of ultraviolet light, photoinactivation in the presence of photosensitizers (e.g., neutral red or methylene blue), and culture under specific conditions...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
Ying Jiang, Jing Jiang, Haiqing Jia, Zhiwei Qiao, Jingru Zhang
BACKGROUND/AIMS: In platinum-based chemotherapy for ovarian cancer, acquired drug resistance is a frequent occurrence. Because recent studies have demonstrated that dysregulation of microRNAs (miRNAs) is partly responsible for the induction of acquired drug resistance in cancers, we hypothesized that correcting the dysregulation of key miRNAs would reverse the acquired resistance to platinum-based drugs in ovarian cancer. METHODS: Cisplatin-resistant SKOV3 and A2780 ovarian cancer cell lines (SKOV3-R and A2780-R, respectively) were established by long-term exposure to cisplatin...
November 15, 2018: Cellular Physiology and Biochemistry
Zhi Huang, Wei Zhou, Yongtao Li, Mei Cao, Tianqi Wang, Yakun Ma, Qingxiang Guo, Xin Wang, Chao Zhang, Chenglan Zhang, Wenzhi Shen, Yanhua Liu, Yanan Chen, Jianyu Zheng, Shengyong Yang, Yan Fan, Rong Xiang
Despite initial progress in preclinical models, most known histone deacetylase inhibitors (HDACis) used as a single agent have failed to show clinical benefits in nearly all types of solid tumours. Hence, the efficacy of HDACis in solid tumours remains uncertain. Herein, we developed a hybrid HDAC inhibitor that sensitized solid tumours to HDAC-targeted treatment. Methods: A hybrid molecule, Roxyl-zhc-84 was designed and synthesized with novel architecture. The pharmacokinetics and toxicity of Roxyl-zhc-84 were analysed...
2018: Theranostics
Kai Lu, Jinming Zhou, Xia Chen, Wenru Li, Yue Li, Yibei Cheng, Jing Yan, Keke You, Zhineng Yuan, Qiang Zhou
Provisioning of sufficient lipids and vitellogenin to the oocytes is an indispensable process for fecundity of oviparous insects. Acute mobilization of lipid reserves in insects is controlled by the Brummer (Bmm), an orthologous of human adipose triglyceride lipase (ATGL). To investigate the functional roles of brummer-mediated lipolysis in the fecundity of the brown planthopper, Nilaparvata lugens , RNA interference (RNAi) analyses were performed with double-stranded RNA (dsRNA) against NlBmm in adult females...
2018: Frontiers in Physiology
Gabriel Levin, Tatiane Maldonado Coelho, Nathali Guimarães Nóbrega, Marina Trombetta-Lima, Mari Cleide Sogayar, Ana Claudia Oliveira Carreira
BACKGROUND: Matrix metalloproteinases (Mmps) and their tissue inhibitors (Timps) are widely recognized as crucial factors for extracellular matrix remodeling in the ovary and are involved in follicular growth, ovulation, luteinization, and luteolysis during the estrous cycle. Recently, several genes have been associated to the modulation of Mmps activity, including Basigin (Bsg), which induces the expression of Mmps in rat ovaries; Sparc, a TGF-β modulator that is related to increased expression of Mmps in cancer; and Reck, which is associated with Mmps inhibition...
November 13, 2018: Reproductive Biology and Endocrinology: RB&E
Upendra Kumar Soni, Sangappa Basanna Chadchan, Vijay Kumar, Vaibhave Ubba, Mohammad Tariq Ali Khan, Budai Shanmukha Vivek Vinod, Rituraj Konwar, Himangsu Kousik Bora, Srikanth Kumar Rath, Sharad Sharma, Rajesh Kumar Jha
Endometriosis is a prevalent gynecological disorder that eventually gives rise to painful invasive lesions. Increased levels of transforming growth factor-beta1 (TGF-B1) have been reported in endometriosis. However, details of the effects of high TGF-B1 on downstream signaling in ectopic endometrial tissue remain obscure. We induced endometriotic lesions in mice by surgical auto-transplantation of endometrial tissues to the peritoneal regions. We then treated endometriotic (ectopic and eutopic endometrial tissues) and non-endometriotic (only eutopic endometrial tissues) animal groups with either active TGF-B1 or PBS...
November 13, 2018: Biology of Reproduction
Yusuke Kobayashi, Kouji Banno, Haruko Kunitomi, Shimpei Nagai, Takayuki Takahashi, Mayuka Anko, Moito Iijima, Takashi Takeda, Yusuke Matoba, Kanako Nakamura, Kosuke Tsuji, Eiichiro Tominaga, Daisuke Aoki
Novel pharmacological therapies are in development for cancer, ranging from conventional chemotherapeutic drugs to molecular targeted drugs, antibody-based drugs, and immune checkpoint inhibitors, which are developed using new technologies. However, the increasing cost of new drug development is increasing the costs of national healthcare and putting pressure on government finances worldwide. Under these circumstances, drug repositioning (i.e. discovering novel effects of existing drugs, thereby allowing their use to treat other diseases) has become a major focus because of reliability and cost reduction...
November 8, 2018: European Journal of Cancer Prevention
Xia Zhao, Zhaofang Cheng, Jian Wang
BACKGROUND Long noncoding RNAs (lncRNAs) have been acknowledged as important regulators in human cancers, including ovarian cancer. Several reports identified lncRNA FEZF1-AS1 as an oncogene in gastric cancer, colorectal carcinoma, and non-small cell lung cancer (NSCLC). However, the function of FEZF1-AS1 in ovarian cancer remains largely unknown. This study was aimed to investigate the role of FEZF1-AS1 in ovarian cancer. MATERIAL AND METHODS FEZF1-AS1 expression levels in pairs of ovarian cancer tissues and adjacent normal tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR)...
November 12, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Poonam Singh, Moumita Sarkar, Usha Agrawal, Ilpo Huhtaniemi, Rahul Pal
The beta subunit of human chorionic gonadotropin (βhCG) is secreted by various tumors, and its presence associated with poor prognosis. Though exogenous hCG elicits the synthesis of molecules associated with angiogenesis, invasion, immune suppression and chemoresistance from responsive tumor cells in vitro , the influence of cell-extrinsic βhCG on tumorigenesis in vivo has not been adequately explored. Female C57BL/6-/- × FVBβhCG/- F1 transgenic mice demonstrated ovarian hyperplasia and pituitary adenomas; transcripts of hCG-driven, tumor-associated molecules were heightened in the pituitary...
October 5, 2018: Oncotarget
Marie Devillers, Florence Petit, Victoria Cluzet, Charlotte M François, Frank Giton, Ghislaine Garrel, Joëlle Cohen-Tannoudji, Celine J Guigon
Anti-Müllerian hormone (AMH) regulates ovarian function in cyclic females, notably by preventing premature FSH-mediated follicular growth and steroidogenesis. Its expression in growing follicles is controlled by FSH and by estradiol (E2). In infantile females, there is a transient increase in the activity of the gonadotrope axis, as reflected by elevated levels of both gonadotropins and E2. We previously demonstrated in mice that elevated FSH concentrations are necessary to induce E2 production by preantral/early antral follicles through the stimulation of aromatase expression without supporting their growth...
November 1, 2018: Journal of Endocrinology
Yang Mi, Rajendra Kumar Gurumurthy, Piotr K Zadora, Thomas F Meyer, Cindrilla Chumduri
Cervical and ovarian cancers exhibit characteristic mutational signatures that are reminiscent of mutational processes, including defective homologous recombination (HR) repair. How these mutational processes are initiated during carcinogenesis is largely unclear. Chlamydia trachomatis infections are epidemiologically associated with cervical and ovarian cancers. Previously, we showed that C. trachomatis induces DNA double-strand breaks (DSBs) but suppresses Ataxia-telangiectasia mutated (ATM) activation and cell cycle checkpoints...
November 6, 2018: MBio
Ling Ding, Xi Chen, Xiaqing Xu, Yuli Qian, Guikai Liang, Fengqi Yao, Zhangting Yao, Honghai Wu, Jieqiong Zhang, Qiaojun He, Bo Yang
Studies have pointed to a role of PARP1 in regulating gene expression through poly(ADP-ribosyl)ating, sequence-specific, DNA-binding transcription factors. However, few examples exist that link this role of PARP1 to the immunogenicity of cancer cells. Here, we report that PARP1 poly(ADP-ribosyl)ates STAT3 and subsequently promotes STAT3 de-phosphorylation, resulting in reduced transcriptional activity of STAT3 and expression of PD-L1. In this study, we showed that PARP1 silencing or pharmacological inhibition enhanced the transcription of PD-L1 in cancer cells, which was accompanied by the upregulation of PD-L1 protein expression, both in the cytoplasm and on the cell surface...
November 6, 2018: Cancer Immunology Research
Claire Glister, Sheena L Regan, Moafaq Samir, Pg Knight
Bone morphogenetic proteins (BMP) are firmly implicated as intra-ovarian regulators of follicle function and steroidogenesis but information is lacking regarding the regulation of BMP signalling by extracellular binding proteins co-expressed in the ovary. In this study we compared the abilities of four BMP binding proteins (gremlin, noggin, chordin, follistatin) to antagonize the action of four different BMPs (BMP2 BMP4, BMP6, BMP7) on LH-induced androstenedione secretion by bovine theca cells in primary culture...
October 1, 2018: Journal of Molecular Endocrinology
Jing Jiang, Chuanmei Xie, Yujuan Liu, Qi Shi, Yan Chen
MicroRNAs are small non-coding RNAs which play important roles in tumor progression. MiR-383-5p has been characterized as a cancer suppressor in several cancers. The aim of theses present study was to explore the role of miR-383-5p in the proliferation and chemosensitivity of ovarian cancer cells. MiR-383-5p expression was down-regulated while the expression of TRIM27 was up-regulated in ovarian cancer tissues and cell lines. We came up with the hypothesis that miR-383-5p might be involved in the tumor progression and chemoresistance of ovarian cancer through targeting TRIM27...
November 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jieru Zhou, Xiaoduan Li, Xiaoli Wu, Ting Zhang, Qinyi Zhu, Xinjing Wang, Husheng Wang, Kai Wang, Yingying Lin, Xipeng Wang
The immune microenvironment is crucial for epithelial ovarian cancer (EOC) progression and consists of tumor-associated macrophages (TAMs) and T lymphocytes, such as regulatory T cells (Tregs) and T helper 17 (Th17) cells. In this study, the Treg/Th17 ratio was significantly higher in EOC in situ and in metastatic peritoneal tissues than in benign ovarian tumors and benign peritoneum. The Treg/Th17 ratio was associated with histological grade and was an independent prognostic factor for overall survival of EOC patients...
November 5, 2018: Cancer Immunology Research
Stine Aagaard Lunding, Susanne Elisabeth Pors, Stine Gry Kristensen, Claus Yding Andersen, Janni Vikkelsø Jeppesen, Kirsten Tryde Macklon, Anders Nyboe Andersen, Anette Tønnes Pedersen
OBJECTIVE: To describe and demonstrate a simple and secure procedure for laparoscopic autotransplantation of fragmented ovarian cortical tissue in women with diminished ovarian reserve as part of in vitro activation (IVA) of ovarian follicles. DESIGN: Step-by-step video explanation of the surgical procedure with still pictures and surgical video clips to demonstrate the detailed technique. SETTING: Fertility clinic and obstetrics and gynecology department at a university hospital...
November 2018: Fertility and Sterility
Xiaojing Li, Hongmin Wang, Jie Ding, Shaozhen Nie, Li Wang, Lili Zhang, Shaoda Ren
Ovarian cancer is one of the most serious diseases worldwide and the fifth-most common cancer among women. Celastrol, extracted from Thunder God Vine, exerts anti-cancer effects on various cancers; however, the mechanism underlying these anti-cancer effects in ovarian cancer needs further investigation. Herein, we investigated the anti-cancer efficacy of celastrol and its underlying mechanism in human ovarian cancer cell lines A2780, OVCAR3, and SKOV3. Celastrol significantly suppressed cell proliferation and migration in a dose-dependent manner...
October 30, 2018: European Journal of Pharmacology
Xiaoping Pan, Chen Wang, Yan Li, Lida Zhu, Ti Zhang
AIM: Although the anti-neoplastic effects of physcion are well documented, its specific action in hepatocellular carcinoma (HCC) is not understood. Taken together, physcion is a promising drug candidate for the treatment and prevention of HCC. MATERIAL AND METHODS: Cell Counting Kit-8 (CCK-8) assay utilized to examine the viability of Hep3B and SMMC7721 cells. Apoptotic cell population was measured by flow cytometry analysis assay. Cell migration and cell invasion were determined by wound healing and Transwell assay...
October 30, 2018: Life Sciences
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