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https://www.readbyqxmd.com/read/30025396/role-of-fgf-2-transfected-bone-marrow-mesenchymal-stem-cells-in-engineered-bone-tissue-for-repair-of-avascular-necrosis-of-femoral-head-in-rabbits
#1
Fei Zhang, Wu-Xun Peng, Lei Wang, Jian Zhang, Wen-Tao Dong, Jian-Hua Wu, Huai Zhang, Jian-Bo Wang, Yin Zhao
BACKGROUND/AIMS: Avascular necrosis of the femoral head (ANFH) is the focus and difficulty of orthopedic diseases. Recently, tissue engineering bone for this disease has shown a good therapeutic effect. The aim of the present study was to investigate the therapeutic effect of basic fibroblast growth factor (FGF-2) as cytokines transfected bone marrow mesenchymal stem cells (BMSCs) in constructing tissue-engineered bone for avascular necrosis of the femoral head. METHODS: The FGF-2 gene overexpressed lentivirus-transfected rBMSCs with xenogeneic antigen-extracted cancellous bone (XACB) to construct tissue engineered bone, and the model of early avascular necrosis of the femoral head was established by lipopolysaccharide (LPS) combined with hormone...
July 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30025278/outcomes-and-mutational-analysis-of-patients-with-lower-risk-non-del5q-myelodysplastic-syndrome-treated-with-antithymocyte-globulin-with-or-without-ciclosporine-a
#2
C Kelaidi, T Braun, R Arana, A Marceau-Renaut, G Lazarian, J Soret, S Cereja, R Letestu, V Eclache, D Lusina, F Baran-Marszak, L Ades, C Preudhomme, A Martin, P Fenaux, C Gardin
Immunosuppressive treatment is a disease-modifying therapy for lower-risk myelodysplastic syndromes (MDS). However, IST is relatively rarely used and long-term outcomes of patients are seldom reported. We retrospectively studied outcomes of 20 patients with lower-risk non del 5q MDS with transfusion dependency, with horse or rabbit antithymocyte globulin ± ciclosporine A, and frontline eltrombopag in two of them. IPSS-R was low, intermediate and high in 30%, 55% and 10% of the patients, respectively. Fifty-five percent of the patients had hypocellular bone marrow (BM)...
May 29, 2018: Leukemia Research
https://www.readbyqxmd.com/read/30025134/new-human-organotypic-corneal-tissue-model-for-ophthalmic-drug-delivery-studies
#3
Yulia Kaluzhny, Miriam W Kinuthia, Thoa Truong, Allison M Lapointe, Patrick Hayden, Mitchell Klausner
Purpose: The purpose of the current work was to develop a physiologically relevant, in vitro human three-dimensional (3D) corneal epithelial tissue model for use in ophthalmic drug development. Methods: Normal human corneal epithelial cells were cultured at the air-liquid interface to produce the 3D corneal tissue model. Corneal barrier was determined by measuring transepithelial electrical resistance (TEER). Quantitative PCR arrays were utilized to investigate expression of 84 phase I/II metabolizing enzymes and 84 drug transporter genes...
June 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/30024993/impact-of-surface-chemistry-modifications-on-speed-and-strength-of-osseointegration
#4
Hyeon-Goo Kang, Yong-Soo Jeong, Yoon-Hyuk Huh, Chan-Jin Park, Lee-Ra Cho
PURPOSE: To compare the bone responses of chemically modified implants using the plasma immersion ion implantation and deposition method with those of blasted implants. MATERIALS AND METHODS: The titanium implants were blasted with resorbable blasting media (RBM) and designated as controls. The ion-implanted implants were divided into two test groups, namely, calcium (Ca) and magnesium (Mg) implants. Six implants (two implants per group) were placed into the proximal tibias of 11 New Zealand white rabbits...
July 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/30024170/metabolic-response-in-rabbit-urine-to-occurrence-and-relief-of-unilateral-ureteral-obstruction
#5
Zhenzhao Wang, Rui Xu, Guiping Shen, Jianghua Feng
Ureteral obstruction will lead clinically to hydronephrosis, which may further develop into partial or complete loss of kidney function and even cause permanent histological damage. However, there is little knowledge of metabolic responses during the obstructed process and its recoverability. In this study, a complete unilateral ureteral obstruction (CUUO) model was established in rabbit, and 1H NMR-based metabolomic analysis of urine was used to reveal the metabolic perturbations in rabbits caused by CUUO and the metabolic recovery after the CUUO was relieved...
July 19, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/30023801/collagen-hydroxypropyl-methylcellulose-membranes-for-corneal-regeneration
#6
Yuyu Long, Xuan Zhao, Sa Liu, Min Chen, Bingqian Liu, Jian Ge, Yong-Guang Jia, Li Ren
To improve intraocular transparency of collagen matrices, hydroxypropyl methylcellulose (HPMC) was introduced for the first time into cross-linked collagen to form collagen-HPMC composite membranes. Light transmittance and refractive indices of the membranes are enhanced by incorporation of HPMC in comparison to the control of cross-linked collagen membranes. Maximum light transmittance of the collagen-HPMC membrane was up to 92%. In addition, their permeability of nutrients such as glucose, tryptophan, and NaCl was superior or comparable to that of human corneas...
January 31, 2018: ACS Omega
https://www.readbyqxmd.com/read/30022821/montmorillonite-chitosan-nanoparticles-as-a-novel-controlled-release-topical-ophthalmic-delivery-system-for-the-treatment-of-glaucoma
#7
Juan Li, Shuangyan Tian, Qi Tao, Yawen Zhao, Ruyi Gui, Fan Yang, Lingquan Zang, Yanzhong Chen, Qineng Ping, Dongzhi Hou
Background: To date, the rapid clearance from ocular surface has been a huge obstacle for using eye drops to treat glaucoma, since it has led to the short preocular residence time and low bioavailability. Methods: The novel nanoparticles (NPs) were designed for topical ophthalmic controlled drug delivery system through intercalating the BH into the interlayer gallery of Na-montmorillonite (Na+Mt) and then further enchasing chitosan nanoparticles. The resulting nanoparticles had a positive charge (+29±0...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/30022811/development-of-a-tin-oxide-carrier-with-mesoporous-structure-for-improving-the-dissolution-rate-and-oral-relative-bioavailability-of-fenofibrate
#8
Andi Bai, Chao Wu, Xuan Liu, Huiling Lv, Xiaoyan Xu, Yue Cao, Wenjing Shang, Lili Hu, Ying Liu
Background: Biopharmaceutics classification system class II drugs have low solubility, which limits their extent and speed of absorption after oral administration. Over the years, mesoporous materials have been widely used to increase the dissolution rate and oral relative bioavailability of poorly water-soluble drugs. Objectives: In order to improve the dissolution rate and increase oral relative bioavailability of the poorly water-soluble drugs, a tin oxide carrier (MSn) with a mesoporous structure was successfully synthesized...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/30022102/segmental-additive-tissue-engineering
#9
Martina Sladkova, Rawan Alawadhi, Rawan Jaragh Alhaddad, Asmaa Esmael, Shoug Alansari, Munerah Saad, Jenan Mulla Yousef, Lulwa Alqaoud, Giuseppe Maria de Peppo
Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation...
July 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30021649/is-exclusion-of-leukocytes-from-platelet-rich-plasma-prp-a-better-choice-for-early-intervertebral-disc-regeneration
#10
Shan-Zheng Wang, Wei-Min Fan, Jun Jia, Liang-Yu Ma, Jia-Bin Yu, Chen Wang
BACKGROUND: Platelet-rich plasma (PRP) is becoming a promising strategy to treat early intervertebral disc degeneration (IDD) in clinics. Pure PRP without leukocytes (P-PRP) may decrease the catabolic and inflammatory changes in the early degenerated intervertebral discs. The aim of this study was to investigate the effects of P-PRP on nucleus pulposus-derived stem cells (NPSCs) isolated from early degenerated intervertebral discs in vitro. METHODS: NPSCs isolated from early degenerated discs of rabbits were treated with P-PRP or leukocyte-platelet-rich PRP (L-PRP) in vitro, followed by measuring cell proliferation, stem cell marker expression, inflammatory gene expression, and anabolic and catabolic protein expression by immunostaining, quantitative real-time polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay...
July 18, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/30021471/recombinant-human-c1-esterase-inhibitor-conestat-alfa-for-prophylaxis-to-prevent-attacks-in-adult-and-adolescent-patients-with-hereditary-angioedema
#11
Anna Valerieva, Sonia Caccia, Marco Cicardi
Hereditary angioedema (HAE) due to C1 inhibitor (C1-INH) deficiency is a debilitating and potentially lethal disease. Management includes on-demand treatment of angioedema and their prophylaxis. Plasma derived C1-INH is an established treatment for both on demand and prophylaxis of HAE. Conestat alfa is a recombinant form of human C1-INH (rhC1-INH) produced in transgenic rabbits. It has granted drug's registration as treatment option for acute HAE attacks in adults and adolescents in Europe, America, and other countries...
July 19, 2018: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/30021387/angiopoietin-2-promotes-angiogenesis-in-tissue-engineered-bone-and-improves-repair-of-bone-defects-by-inducing-autophagy
#12
Jian Yin, Ge Gong, Chao Sun, Zhaoyang Yin, Chao Zhu, Bin Wang, Qin Hu, Yuerong Zhu, Xinhui Liu
Angiogenesis plays a key role in the repair of large segmental bone defects with tissue-engineered bones. However, there is no effective method of promoting angiogenesis in tissue-engineered bone. Both angiopoietin 2 (Ang2) and autophagy have been shown to be involved in angiogenesis, but their roles in angiogenesis of tissue-engineered bone remains unknown. In this in vivo study, a radius bone defect was created in New Zealand white rabbits, which were then treated by implantation of a hydroxyapatite/collagen scaffold followed by injection of different concentrations of Ang2...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/30020792/amino-functionalized-mesoporous-silica-particles-for-ocular-delivery-of-brimonidine
#13
Se-Na Kim, Song Ah Ko, Chun Gwon Park, Seung Ho Lee, Beom Kang Huh, Yoh Han Park, Young Kook Kim, Ahnul Ha, Ki Ho Park, Young Bin Choy
To treat glaucoma, conventional eye drops are often prescribed. However, the eye drops have limited effectiveness as a result of low drug bioavailability due to their rapid clearance from the preocular space. To resolve this, we proposed amino-functionalized mesoporous silica (AMS) particles as delivery carriers of the glaucoma drug, brimonidine. Because of the presence of mesopores, brimonidine (BMD) could be encapsulated in the AMS with a loading amount of 41.73 μg/mg (i.e., drug loading capacity of about 4...
July 18, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/30020409/can-the-impact-of-topiramate-on-memory-processes-be-related-to-its-antialcoholic-activity-a-preclinical-study
#14
Ewa Zwierzynska, Agata Krupa-Burtnik, Agnieszka Wiesner, Boguslawa Pietrzak
Aims: Topiramate causes the inhibition of alcohol consumption in addicts but the mechanism of this action has not been fully understood yet. Nowadays, it seems that memory may have a role in the development of dependence. In this study, the impact of topiramate and ethanol on the bioelectric activity of the brain in rabbits and on spatial memory in rats was evaluated. Short summary: The aim of the study was to assess the effect of co-administration of topiramate and ethanol on bioelectric activity of the rabbits' brain and on spatial memory in rats...
July 18, 2018: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/30019997/retrospective-survey-of-owners-experiences-with-palliative-radiation-therapy-for-pets
#15
Vivian C Fan, Monique N Mayer, Sally L Sukut, Nancy R Gustafson, G Neal Mauldin, Cheryl L Waldner
OBJECTIVE To describe animal owners' experiences with palliative radiation therapy (PRT) of pets and identify factors influencing satisfaction with their pets' treatment. DESIGN Retrospective, cross-sectional study. SAMPLE 118 owners of dogs, cats, or rabbits. PROCEDURES Medical records were searched to identify animals that underwent PRT between 2004 and 2013. Signalment, tumor-related data, and outcome information were recorded. Owners completed an electronic survey assessing satisfaction with treatment (ie, satisfaction with the decision for their pet to undergo PRT and indication that they would choose PRT for their pet again), expectations regarding PRT, and perceptions of their pets' quality of life (QOL) and signs of discomfort from acute adverse radiation effects...
August 1, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/30019963/rapid-and-sensitive-detection-of-campylobacter-jejuni-in-poultry-products-using-a-nanoparticle-based-piezoelectric-immunosensor-integrated-with-magnetic-immunoseparation
#16
Hong Wang, Lijun Wang, Qinqin Hu, Ronghui Wang, Yanbin Li, Michael Kidd
Campylobacter jejuni is one of the leading causes of foodborne human gastrointestinal diseases. Poultry and poultry products have been identified as the major transmission routes to humans for this pathogenic bacterium. The objective of this research was to develop a rapid and sensitive immunosensor for detection of C. jejuni in poultry products on the basis of a quartz crystal microbalance (QCM) using magnetic nanobeads (MNBs) for separation of target pathogen and gold nanoparticles for amplification of the measurement...
July 16, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/30019852/effects-of-maternal-stress-induced-by-predator-odors-during-gestation-on-behavioral-and-physiological-responses-of-offspring-in-brandt-s-vole-lasiopodomys-brandi
#17
Chen Gu, Wenjia Wang, Xiaoqian Ding, Shengmei Yang, Aiqin Wang, Baofa Yin, Wanhong Wei
Maternal effects play a crucial role in regulating populations of small mammals and anti-predator behaviors of offspring. This study investigated the effects of maternal stress induced by exposure to predator odors during gestation on the behavioral and physiological responses of offspring in Brandt's vole (Lasiopodomys brandtii). Effects included changes in growth, behaviors, hormone levels in serum, and fosB/ΔfosB mRNA and FosB/ΔFosB protein expression in the hypothalamus. Our results showed that when pregnant voles were exposed to cat and rabbit urine odors for 18 days (1 h/day), the weight of the female offspring decreased; however, the thymus and ovary indices increased, compared with the control group...
July 18, 2018: Integrative Zoology
https://www.readbyqxmd.com/read/30019623/traumatic-injury-leads-to-inflammation-and-altered-tryptophan-metabolism-in-the-juvenile-rabbit-brain
#18
Zhi Zhang, Lindsey Rasmussen, Manda Saraswati, Raymond Koehler, Courtney L Robertson, Sujatha Kannan
Neuroinflammation following traumatic brain injury (TBI) contributes to widespread cell death and tissue loss. Here we evaluated the sequential inflammatory response in the brain, as well as inflammation-induced changes in brain tryptophan metabolism over time in a rabbit pediatric TBI model. On postnatal day 5-7 (P5-7), New Zealand white rabbit littermates were randomized into three groups, naïve (no injury), sham (craniotomy alone) and TBI (controlled cortical impact). Animals were sacrificed at 6 h, 1, 3, 7 and 21 days post-injury for evaluating levels of pro- and anti-inflammatory cytokines, as well as the major components in the tryptophan-kynurenine pathway...
July 18, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/30019315/differing-effects-of-parathyroid-hormone-alendronate-and-odanacatib-on-bone-formation-and-on-the-mineralization-process-in-intracortical-and-endocortical-bone-of-ovariectomized-rabbits
#19
Christina Vrahnas, Pascal R Buenzli, Thomas A Pearson, Brenda L Pennypacker, Mark J Tobin, Keith R Bambery, Le T Duong, Natalie A Sims
Bone is formed by deposition of a collagen-containing matrix (osteoid) that hardens over time as mineral crystals accrue and are modified; this continues until bone remodeling renews that site. Pharmacological agents for osteoporosis differ in their effects on bone remodeling, and we hypothesized that they may differently modify bone mineral accrual. We, therefore, assessed newly formed bone in mature ovariectomized rabbits treated with the anti-resorptive bisphosphonate alendronate (ALN-100µ g/kg/2×/week), the anabolic parathyroid hormone (PTH (1-34)-15µ g/kg/5×/week), or the experimental anti-resorptive odanacatib (ODN 7...
July 17, 2018: Calcified Tissue International
https://www.readbyqxmd.com/read/30019187/a-promising-drug-candidate-for-the-treatment-of-glaucoma-based-on-a-p2y6-receptor-agonist
#20
Tali Fishman Jacob, Vijay Singh, Mudit Dixit, Tamar Ginsburg-Shmuel, Begoña Fonseca, Jesus Pintor, Moussa B H Youdim, Dan T Major, Orly Weinreb, Bilha Fischer
Extracellular nucleotides can regulate the production/drainage of the aqueous humor via activation of P2 receptors, thus affecting the intraocular pressure (IOP). We evaluated 5-OMe-UDP(α-B), 1A, a potent P2Y6-receptor agonist, for reducing IOP and treating glaucoma. Cell viability in the presence of 1A was measured using [3-(4, 5-dimethyl-thiazol-2-yl) 2, 5-diphenyl-tetrazolium bromide] (MTT) assay in rabbit NPE ciliary non-pigmented and corneal epithelial cells, human retinoblastoma, and liver Huh7 cells...
July 17, 2018: Purinergic Signalling
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