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Patricia A Pesavento, Cristina W Cunha, Hong Li, Kenneth Jackson, Donal O'Toole
A constraint on understanding the pathogenesis of malignant catarrhal fever (MCF) is the limited number of tools to localize infected cells. The amount of detectable virus, visualized in the past either by immunohistochemistry or in situ hybridization (ISH), has been modest in fixed or frozen tissues. This complicates our understanding of the widespread lymphoid proliferation, epithelial necrosis/apoptosis, and arteritis-phlebitis that characterize MCF. In this work, we developed a probe-based in situ hybridization assay targeting 2 ovine herpesvirus 2 (OvHV-2) genes, as well as their respective transcripts, in formalin-fixed tissues...
September 16, 2018: Veterinary Pathology
Xiao Lin, Rajeev K Naidu, Jinhui Dai, Xingtao Zhou, Xiaomei Qu, Hao Zhou
Background This study aims to assess the efficacy of the scleral collagen cross-linking method using glyceraldehyde solution for prevention of lens-induced axial elongation in New Zealand rabbits, and investigate the biochemical and microstructural changes that occur. Methods The right eyes of New Zealand rabbits aged seven weeks were randomly divided into three groups: the cross-linking group (n = 6), non-crosslinking group (n = 5), and untreated control group (n = 5). Eyes in cross-linking and non-crosslinking groups were treated with a -8...
September 17, 2018: Current Eye Research
Shiyu Dong, Nicole D Wagner, David H Russell
Metallothioneins (MTs) are a group of intrinsically disordered proteins that exhibit extreme diversity in structure, biological functionality, and metal ion specificity. Structure(s) of coordinatively saturated metalated MTs have been extensively studied, but there exists very limited structural information for the partially metalated MTs. Here, the conformational preferences of partial metalated rabbit metallothionein-2A (MT) by Cd2+ , Zn2+ , and Ag+ is studied using nano-electrospray ionization-ion mobility-mass spectrometry (nESI-IM-MS)...
September 17, 2018: Analytical Chemistry
Shoudong Chai, Yang Yu, Haiming Li, Mingxin Gao, Li Bo, Chengxiong Gu
Vein graft remains the most broadly applied vascular material in coronary artery bypass surgery. However, the restenosis rate of the vein bridge following angioplasty is high. The present study investigated the effect of medical adhesive on vascular intimal hyperplasia, in addition to the signal transduction mechanism. A total of 36 New Zealand white rabbits were divided into three groups at random, including the normal group, the surgery group and the medical adhesive spray group. Following surgery for transplantation of the left external jugular vein to the ipsilateral common carotid artery for 4 weeks, the thickness and area of the intima and media of the vessel were measured on formalin‑fixed, paraffin wax‑embedded pathological sections using hematoxylin‑eosin staining, and alterations in the expression of proliferating cell nuclear antigen (PCNA), platelet endothelial cell adhesion molecule 1 (PECAM‑1), vascular cell adhesion protein 1 (VCAM‑1), extracellular signal‑regulated kinase (ERK)1/2, and endothelial nitric oxide synthase (eNOS) were detected by immunohistochemical staining, reverse transcription‑quantitative polymerase chain reaction analysis and western blotting...
September 17, 2018: Molecular Medicine Reports
Chun-Chien Shieh, Jeffrey Barber, William Counter, Jonathan R Sykes, Peter Bennett, Soo-Min Heng, Paul White, Stéphanie Corde, Michael Jackson, Verity Ahern, Ilana J Feain, Ricky T O'Brien, Paul J Keall
Fixed-gantry cone-beam computed tomography (CBCT), where the imaging hardware is fixed while the subject is continuously rotated 360$\degree$ in the horizontal position, has implications for building compact and affordable fixed-gantry linear accelerators (linacs). Fixed-gantry imaging with a rotating subject presents a challenging image reconstruction problem where the gravity-induced motion is coupled to the subject's rotation angle. This study is the first to investigate the feasibility of fixed-gantry CBCT using imaging data of three live rabbits in an ethics-approved study...
September 17, 2018: Physics in Medicine and Biology
Rongquan Duan, Davide Barbieri, Florence de Groot, Joost D de Bruijn, Huipin Yuan
Tricalcium phosphate (TCP) ceramics are used as bone void fillers because of their bioactivity and resorbability, while their performance in bone regeneration and material resorption vary with their physical properties (e.g., the dimension of the crystal grain). Herein, three TCP ceramic bone substitutes (TCP-S, TCP-M, and TCP-L) with gradient crystal grain size (0.77 ± 0.21 μm for TCP-S, 1.21 ± 0.35 μm for TCP-M and 4.87 ± 1.90 μm for TCP-L), were evaluated in a well-established rabbit lateral condylar defect model (validated with sham) with respect to bone formation and material resorption up to 26 weeks...
September 10, 2018: ACS Biomaterials Science & Engineering
Mansi Purwar, Jonathan K Pokorski, Pranveer Singh, Sanchari Bhattacharyya, Heather Arendt, Joanne DeStefano, Celia C La Branche, David C Montefiori, M G Finn, Raghavan Varadarajan
The broadly neutralizing antibody against HIV-1, b12, binds to the CD4 binding site (CD4bs) on the outer domain (OD) of the gp120 subunit of HIV-1 Env. We have previously reported the design of an E. coli expressed fragment of HIV-1 gp120, b122a, containing about 70% of the b12 epitope with the idea of focusing the immune response to this structure. Since the b122a structure was found to be only partially folded, as assessed by circular dichroism and protease resistance, we attempted to stabilize it by the introduction of additional disulfide bonds...
September 14, 2018: Vaccine
Sabrina Ruchti, Andrea R Meier, Hanno Würbel, Gilles Kratzer, Sabine G Gebhardt-Henrich, Sonja Hartnack
In rabbits (Oryctolagus cuniculus L.), pododermatitis is a chronic multifactorial skin disease that appears mainly on the plantar surface of the hind legs. In later stages, it causes pain leading to poor welfare of affected animals. Pododermatitis is commonly observed in commercial rabbit production in breeding does housed with wire mesh flooring. However, the prevalence in breeding does that are housed in groups on litter and plastic slats is not known. The aim of this cross-sectional study was to investigate the frequency, the severity and possible risk factors of pododermatitis in group housed breeding does in Switzerland on litter and plastic slats...
October 1, 2018: Preventive Veterinary Medicine
Shun Zhang, Xiang Shu, Jinji Yang, Jinyue Shi, Xiaomei Guan, Jianrong Jiang, Yingming Wei, Chan Luo, Deshun Shi, Fenghua Lu
The present study explored a suitable parthenogenetic activation (PA) procedure for rabbit oocytes and investigated the developmental potential of somatic cell nuclear transfer (SCNT) embryos using rabbit fetal fibroblasts (RFFs). The electrical activation had the optimal rate of blastocyst (14.06%) when oocytes were activated by three direct current (DC) pulses (40 V/mm, 20 μs each) followed by 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) treatment; the blastocyst rate of ionomycin (ION)+6-DMAP+CHX (12...
September 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
Laura Montes-Medina, Alberto Hernández-Fernández, Araika Gutiérrez-Rivera, Purificación Ripalda-Cemboráin, Nerea Bitarte, Virginia Pérez-López, Froilán Granero-Moltó, Felipe Prosper, Ander Izeta
Acceleration of the consolidation of the distracted bone is a relevant medical need. As a platform to improve in vivo bone engineering, we developed a novel distraction osteogenesis (DO) model in a rabbit large bone (femur) and tested if the application of cultured bone marrow stromal cells (BMSCs) immediately after the osteotomy promotes the formation of bone. This report consists of two components, an animal study to evaluate the quality of the regenerate following different treatments and an in vitro study to evaluate osteogenic potential of BMSC cultures...
September 6, 2018: Injury
Runtai Chen, Zhenchun Huang, Junyi Wang, Xiaoying Chen, Yucai Fu, Wei Wang
BACKGROUND: This study aimed to investigate the interactions between silent information regulator 1 (SIRT1) and mammalian target of rapamycin (mTOR) in intraplaque angiogenesis and their potential mechanisms through in vivo and in vitro studies. METHODS: An atherosclerosis model was established in 12 rabbits on a high-cholesterol diet. The rabbits were equally divided into 3 groups: a control group (high-lipid diet), RAP group (high-lipid diet supplemented with rapamycin) and RAP + NAM group (high-lipid diet supplemented with rapamycin and nicotinamide)...
August 2018: American Journal of the Medical Sciences
Ashirbad Nanda, Rudra Narayan Sahoo, Arunima Pramanik, Rajaram Mohapatra, Sukanta Kumar Pradhan, Arun Thirumurugan, Debajyoti Das, Subrata Mallick
Mucoadhesive type ocular film has been prepared for studying the anti-inflammatory potential of amlodipine (AML) on carrageenan-induced rabbit model and the effect of sulphobutyl-ether-beta-cyclodextrin on corneal permeation was tested. Hydroxypropyl methylcellulose (HPMC) ocular film was prepared after complexation of amlodipine with β-cyclodextrin, (BCD), hydroxypropyl β-cyclodextrin (HPCD), and sulfobutylether β-cyclodextrin (SBCD). The film without cyclodextrin showed a maximum swelling, and erosion to the highest extent...
September 8, 2018: Colloids and Surfaces. B, Biointerfaces
Louise Bunce
Taxidermied animals provide an important source of information about the natural world, but failure to understand their reality status may result in missed learning opportunities for museum visitors. This study explored 4-10-year-old children's and adults' (N = 207) reality status judgments and biological and authenticity property attributions for a taxidermied rabbit during a museum visit. The aim was to examine their understanding of its former status as a living animal and its current status as an authentic piece of natural history, under different presentation conditions: (a) as a touchable exhibit; (b) inside an exhibition case-untouchable; and (c) in an experimental context paired with a toy rabbit-both touchable...
September 12, 2018: Journal of Experimental Child Psychology
Phillip Dixon, Keith Christopher, Anuj Chauhan
Cystinosis is a genetic disease that leads to the accumulation of intracellular cystine crystals in all organs including cornea due to the loss of cystine efflux transporters in the lysosome of the cells. While the mechanism for formation of intracellular cystine is well understood for most organs, it does not explain many observations for crystal accumulation in the cornea of cystinosis patients. First, the crystals in cornea are extracellular and needle-like with several hundred microns length which is in sharp contrast with the rectangular or hexagonal crystals found throughout other organs...
September 12, 2018: International Journal of Pharmaceutics
Zhigang Hu, Xue Yang, Shaoxia Zhuang, Jie Liu
We diagnosed vaccinia virus infection in five male workers directly exposed to rabbit-skin powder contaminated by vaccinia virus via skin contact and inhalation. Four of the workers had been vaccinated against smallpox. Their clinical symptoms were severe, especially in the lungs; however, all symptoms were ameliorated after treatment.
September 12, 2018: International Journal of Infectious Diseases: IJID
Maryam Vejdani-Jahromi, Jenna Freedman, Gregg E Trahey, Patrick D Wolf
OBJECTIVES: Intraventricular pressure (IVP) is one of the most important measurements for evaluating cardiac function, but this measurement is not currently easily assessable in the clinic. The primary reason for this is the absence of a noninvasive technique for measuring IVP. In this study, we investigate the relationship between IVP and dynamic myocardial stiffness measured by shear wave elasticity imaging (SWEI) and assess the feasibility of measuring IVP using SWEI. METHODS: In 8 isolated working rabbit hearts, IVP was recorded in the left ventricle using a pressure catheter...
September 14, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
Dharitri Rath, Satyendra Kumar, Siddhartha Panda
Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of the solution as the target proteins present in the serum samples are charged molecules. Here, we investigated the capture of prostate-specific antigens (PSAs), first in a mixed-analyte system wherein the solution contained two other non-specific proteins along with the target analyte, using the surface plasmon resonance spectroscopy...
September 15, 2018: Applied Biochemistry and Biotechnology
Xu Xu, Jing Yu, Hui Shi, Jie Zhang, Xingyi Li
The antibody avastin® (Ava) has been clinically to treat various intraocular neovascular diseases, but suffering from the rapid clearance and short shelf-life of Ava in the requirement of frequent administration. In the present study, we reports the sustained release of Ava from a thermosensitive hydrogel based on poly(ethylene glycol)-poly(ɛ-caprolactone)-poly(ethylene glycol) (PECE) copolymer for the control of corneal neovascularization in rabbit model. Ava were physically mixed with PECE aqueous solution at 4°C, and resulting Ava-PECE solution showed a sol-gel transition at physiological temperature (37°C)...
September 11, 2018: International Journal of Pharmaceutics
Liang Zhang, Na Wang, Jingsong Mao, Xiaofei Liu, Zhichun Gao, Xu Dai, Bo Feng
PURPOSE: To evaluate whether changes in volumetric iodine concentration (VIC) could serve as a suitable predictor of therapeutic response to microwave (MW) ablation in a rabbit intrahepatic VX2 tumor model. MATERIALS AND METHODS: Sixteen intrahepatic VX2 tumors were transplanted in 8 New Zealand White rabbits treated with MW ablation. Contrast-enhanced dual-energy CT scans were obtained at baseline and follow-up. Therapeutic response assessment by modified Response Evaluation Criteria In Solid Tumors (mRECIST), Choi criteria, and VIC changes was performed...
September 11, 2018: Journal of Vascular and Interventional Radiology: JVIR
Lucie Hympanova, Marina Gabriela Monteiro Carvalho Mori da Cunha, Rita Rynkevic, Radoslaw A Wach, Alicja K Olejnik, Patricia Y W Dankers, Boris Arts, Tristan Mes, Anton W Bosman, Maarten Albersen, Jan Deprest
PURPOSE: Electrospun meshes mimic the extracellular matrix, which may improve their integration. We aimed to compare polycaprolactone (PCL) modified with ureidopyrimidinone (UPy) electrospun meshes with ultra-lightweight polypropylene (PP; Restorelle) reference textile meshes for in vivo compliance. We chose UPy-PCL because we have shown it does not compromise biomechanical properties of native tissue, and because it potentially can be bioactivated. METHODS: We performed ex vivo biomechanical cyclic loading in wet conditions and in vivo overlay of full-thickness abdominal wall defects in rats and rabbits...
August 21, 2018: Journal of the Mechanical Behavior of Biomedical Materials
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