Rebeca Salgado-García, Jossimar Coronel-Hernández, Izamary Delgado-Waldo, David Cantú de León, Verónica García-Castillo, Eduardo López-Urrutia, Ma Concepción Gutiérrez-Ruiz, Carlos Pérez-Plasencia, Nadia Jacobo-Herrera
Colorectal cancer (CRC) is among the top three most deadly cancers worldwide. The survival rate for this disease has not been reduced despite the treatments, the reason why the search for therapeutic alternatives continues to be a priority issue in oncology. In this research work, we tested our successful pharmacological combination of three drugs, metformin, doxorubicin, and sodium oxamate (triple therapy, or TT), as an autophagy inducer. Firstly, we employed western blot (WB) assays, where we observed that after 8 h of stimulation with TT, the proteins Unc-51 like autophagy activating kinase 1(ULK1), becline-1, autophagy related 1 protein (Atg4), and LC3 increased in the CRC cell lines HCT116 and SW480 in contrast to monotherapy with doxorubicin...
February 9, 2021: Genes