Brian Alford, Brian P Landry, Sarah Hou, Xavier Bower, Anna M Bueno, Drake Chen, Brooke Husic, David E Cantonwine, Thomas F McElrath, Jacqueline A Carozza, Julia Wynn, Jennifer Hoskovec, Kathryn J Gray
We developed and validated a next generation sequencing-(NGS) based NIPT assay using quantitative counting template (QCT) technology to detect RhD, C, c, E, K (Kell), and Fya (Duffy) fetal antigen genotypes from maternal blood samples in the ethnically diverse U.S. population. Quantitative counting template (QCT) technology is utilized to enable quantification and detection of paternally derived fetal antigen alleles in cell-free DNA with high sensitivity and specificity. In an analytical validation, fetal antigen status was determined for 1061 preclinical samples with a sensitivity of 100% (95% CI 99-100%) and specificity of 100% (95% CI 99-100%)...
August 7, 2023: Scientific Reports