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Lung microdialysis

B Yang, J D Gao, X Y Cao, Q Y Wang, G Z Sun, J J Yang
For most bacterial lung infections, the concentration of unbound antimicrobial agent in lung interstitial fluid has been considered as the gold standard for estimating the antibacterial efficacy. In this study, the pharmacokinetics of florfenicol (FF) in porcine lung interstitial fluid was investigated after single intramuscular administration at two different doses (20 and 50 mg/kg). Twelve pigs underwent thoracotomy under general anesthesia. Then, the CMA/30 probe was implanted into the lung and perfused at 1 μL/min...
January 19, 2017: Journal of Veterinary Pharmacology and Therapeutics
Paulo Kofuji, Ludovic S Mure, Logan J Massman, Nicole Purrier, Satchidananda Panda, William C Engeland
Light is a powerful entrainer of circadian clocks in almost all eukaryotic organisms promoting synchronization of internal circadian rhythms with external environmental light-dark (LD) cycles. In mammals, the circadian system is organized in a hierarchical manner, in which a central pacemaker in the suprachiasmatic nucleus (SCN) synchronizes oscillators in peripheral tissues. Recent evidence demonstrates that photoentrainment of the SCN proceeds via signaling from a subpopulation of retinal ganglion cells (RGCs) which are melanopsin-expressing and intrinsically photosensitive (ipRGCs)...
2016: PloS One
P M Bernardi, F Barreto, T Dalla Costa
A bioanalytical LC-MS/MS method was developed and validated to determine tobramycin in plasma and lung microdialysate samples. Tobramycin was separated using a C18 column and a mobile phase consisting of water and acetonitrile, both with 10mM of heptafluorobutyric acid, eluted as gradient. Apramycin was used as internal standard (IS) for plasma samples. Drugs were monitored using electrospray ionization operating on positive mode (ESI+) monitoring the transitions 468.2>163.3 m/z for tobramycin and 540.3>217...
February 5, 2017: Journal of Pharmaceutical and Biomedical Analysis
Estevan Sonego Zimmermann, João Victor Laureano, Camila Neris Dos Santos, Stephan Schmidt, Chakradhar V Lagishetty, Whocely Victor de Castro, Teresa Dalla Costa
Levofloxacin (LEV) is a broad-spectrum fluoroquinolone used to treat pneumonia, urinary tract infections, chronic bacterial bronchitis, and prostatitis. Efflux transporters, primarily P-glycoprotein (P-gp), are involved in LEV's tissue penetration. In the present work, LEV free lung and prostate interstitial space fluid (ISF) concentrations were evaluated by microdialysis in Wistar rats after intravenous (i.v.) and intratracheal (i.t.) administration (7 mg/kg of body weight) with and without coadministration of the P-gp inhibitor tariquidar (TAR; 15 mg/kg administered i...
February 2016: Antimicrobial Agents and Chemotherapy
Stephen W Davies, Kenji L Leonard, Randall K Falls, Ronald P Mageau, Jimmy T Efird, Joseph P Hollowell, Wayne E Trainor, Hilal A Kanaan, Robert C Hickner, Robert G Sawyer, Nathaniel R Poulin, Brett H Waibel, Eric A Toschlog
BACKGROUND: Concomitant lung/brain traumatic injury results in significant morbidity and mortality. Lung protective ventilation (Acute Respiratory Distress Syndrome Network [ARDSNet]) has become the standard for managing adult respiratory distress syndrome; however, the resulting permissive hypercapnea may compound traumatic brain injury. Airway pressure release ventilation (APRV) offers an alternative strategy for the management of this patient population. APRV was hypothesized to retard the progression of acute lung/brain injury to a degree greater than ARDSNet in a swine model...
February 2015: Journal of Trauma and Acute Care Surgery
Rong Li, Meiping Ren, Ni Chen, Mao Luo, Xin Deng, Jiyi Xia, Guang Yu, Jinbo Liu, Bing He, Xu Zhang, Zhuo Zhang, Xiao Zhang, Bing Ran, Jianbo Wu
BACKGROUND: Platelets play a fundamental role in maintaining hemostasis and have been shown to participate in hematogenous dissemination of tumor cells. Abundant platelets were detected in the tumor microenvironment outside of the blood vessel, thus, platelet -tumor cell interaction outside of the bloodstream may play a role in regulating primary tumor growth and metastasis initiation. However, it is unclear that platelet depletion affects tumor vessel structure and dynamics. METHODS: Using thrombocytopenia induction in two different tumor-bearing mouse models, tumor tissues were performed by Westernblotting and immunohistochemical staining...
March 10, 2014: BMC Cancer
Yan Zhou, Huimin Wang, Hua Zhong
BACKGROUND AND OBJECTIVE: The survival rate of lung cancer is low, thus new methods for treating this form of cancer must be explored. This study applies immune therapy with metronomic chemotherapy to observe the effect of combined therapy on suppressing tumor. METHODS: Mice were inoculated with Lewis lung carcinoma cells. Different treatments, namely, saline, metronomic chemotherapy, dendritic cell (DC) vaccine, and metronomic chemotherapy with DC vaccine were administered to corresponding mice groups...
September 2013: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
D Hutschala, K Skhirtladze, C Kinstner, M Zeitlinger, W Wisser, W Jaeger, M Hoeferl, M Müller, E Tschernko
The use of cardiopulmonary bypass (CPB) during cardiac surgery causes regional ventilation-perfusion mismatch, contributing to regional disturbances in antibiotic penetration into lung tissue. Ventilation-perfusion mismatch is associated with postoperative pneumonia, a frequent and devastating complication after cardiac surgery. In this prospective clinical animal study, we performed in vivo microdialysis to determine the effect of CPB on regional penetration of levofloxacin (LVX) into lung tissue. Six pigs underwent surgery with CPB (CPB group), and another six pigs underwent surgery without CPB (off-pump coronary artery bypass grafting; OPCAB group)...
July 2013: Antimicrobial Agents and Chemotherapy
Claire Dahyot-Fizelier, Denis Frasca, Nicolas Grégoire, Christophe Adier, Olivier Mimoz, Bertrand Debaene, William Couet, Sandrine Marchand
Central nervous system (CNS) antibiotic distribution was described mainly from cerebrospinal fluid data, and only few data exist on brain extracellular fluid concentrations. The aim of this study was to describe brain distribution of cefotaxime (2 g/8 h) by microdialysis in patients with acute brain injury who were treated for a lung infection. Microdialysis probes were inserted into healthy brain tissue of five critical care patients. Plasma and unbound brain concentrations were determined at steady state by high-performance liquid chromatography...
June 2013: Antimicrobial Agents and Chemotherapy
Bibiana Verlindo de Araújo, João Victor Laureano, Lauren Dockhorn Grünspan, Teresa Dalla Costa, Leandro Tasso
A liquid chromatography method has been established for the reliable determination of unbound gemifloxacin concentrations in kidney, lung and liver microdialysates of rats. Microdialysis probes were inserted into tissues of rats, and then dialysates were collected at regular time intervals after intravenous administration of gemifloxacin (40 mg kg(-1)). A pilot study was performed to assess gemifloxacin penetration in lung, kidney and liver of rats. Gemifloxacin was separated on a C(18) column eluted using triethylamine solution (0...
March 1, 2013: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Xiaoying Cao, Jianning Geng, Suwen Su, Linan Zhang, Qian Xu, Li Zhang, Yinghua Xie, Shaomei Wu, Yongjun Sun, Zibin Gao
A novel drug delivery system of doxorubicin (DOX)-loaded Zein in situ gel for interstitial chemotherapy was investigated in this study. The possible mechanisms of drug release were described according to morphological analysis by optical microscopy and scanning electronic microscope (SEM). In vitro and in vivo anti-tumor activity studies showed that DOX-loaded Zein in situ gel was superior to DOX solution. Local pharmacokinetics in tumor tissue was studied by quantitative analysis with confocal laser scanning microscopy (CLSM) combined with microdialysis technology...
2012: Chemical & Pharmaceutical Bulletin
Wen-shiann Wu, Ming-ting Chou, Chien-ming Chao, Chen-kuei Chang, Mao-tsun Lin, Ching-ping Chang
AIM: To assess the therapeutic effect of melatonin on heat-induced acute lung inflammation and injury in rats. METHODS: Heatstroke was induced by exposing anesthetized rats to heat stress (36 °C, 100 min). Rats were treated with vehicle or melatonin (0.2, 1, 5 mg/kg) by intravenous administration 100 min after the initiatioin of heatstroke and were allowed to recover at room temperature (26 °C). The acute lung injury was quantified by morphological examination and by determination of the volume of pleural exudates, the number of polymorphonuclear (PMN) cells, and the myeloperoxidase (MPO) activity...
June 2012: Acta Pharmacologica Sinica
E Rostami, M Bondi
PURPOSE: The aim of this study is to investigate the effect of local activation of β-adrenoreceptor by Isoprenaline on metabolism in brain, fat and lung measured by microdialysis. METHODS: We used 8 healthy pigs under general anaesthesia and placed microdialysis catheters in brain, fat, lung and artery. We performed a direct measurement of glucose, lactate, pyruvate and glycerol. The stimulation was performed by one-hour infusion of Isoprenaline, a β-adrenoreceptor agonist...
June 1, 2012: Advances in Medical Sciences
Mao Horio, Mami Kohno, Yuko Fujita, Tamaki Ishima, Ran Inoue, Hisashi Mori, Kenji Hashimoto
D-Serine, an endogenous co-agonist of the N-methyl-D-aspartate (NMDA) receptor, plays an important role in mammalian brain neurotransmission, via the NMDA receptor. D-Serine is synthesized from L-serine by the pyridoxal-5' phosphate-dependent enzyme serine racemase (SRR), and D-serine is metabolized by D-amino acid oxidase (DAAO). In this study, we measured levels of the neurotransmission related amino acids, d-serine, L-serine, glycine, glutamine and glutamate in the frontal cortex, hippocampus, striatum and cerebellum as well as in peripheral tissues of blood, heart, pancreas, spleen, liver, kidney, testis, epididymis, heart, lung, muscle and eyeball, in wild-type (WT) and Srr-knockout (Srr-KO) mice...
November 2011: Neurochemistry International
Oliver Mauric, Christiane Thallinger, Sylvia A Kugler, Samir M Joukhadar, Florian M Kovar, Karl H Konz, Wolfgang Graninger, Christian Joukhadar
BACKGROUND/AIMS: We measured the extracellular concentrations of fluconazole in lung tissue of septic and healthy rats. METHODS: A single intravenous dose of 6 mg/kg total body weight of fluconazole was administered intravenously to rats following insertion of microdialysis probes into lung tissue. Another probe was inserted into skeletal muscle and served as control. RESULTS: The mean peak concentration (C(max)), time to C(max), area under the concentration-versus-time curve from 0 to 6 h (fAUC(0-6)) and area under the concentration-versus-time curve from 0 to ∞ of unbound fluconazole for healthy lung were 11...
2011: Pharmacology
Jörg Lindenmann, Sylvia A Kugler, Veronika Matzi, Christian Porubsky, Alfred Maier, Peter Dittrich, Wolfgang Graninger, Freyja M Smolle-Jüttner, Christian Joukhadar
OBJECTIVES: the objective of the present investigation was to measure the extracellular concentrations of cefpirome in unaffected and infected lung tissue of septic patients. METHODS: a single intravenous dose of 30 mg/kg total body weight of cefpirome was administered to eight patients every 12 h prior to insertion of microdialysis probes into lung tissue. RESULTS: the median (minimum, maximum) peak concentration (C(max)), time to C(max) (T(max)), area under the concentration-time curve from 0 to 4 h (AUC(0-4)) and AUC(0-∞) of unbound cefpirome for unaffected lung were 48 (32, 107) mg/L, 0...
January 2011: Journal of Antimicrobial Chemotherapy
Jayesh Dhanani, Jason A Roberts, Michelle Chew, Jeffrey Lipman, Robert J Boots, David L Paterson, John F Fraser
Pneumonia is a form of lung infection that may be caused by various micro-organisms. The predominant site of infection in pneumonia is debatable. Advances in the fields of diagnostic and therapeutic medicine have had a less than optimal effect on the outcome of pneumonia and one of the many causes is likely to be inadequate antimicrobial concentrations at the site of infection in lung tissue. Traditional antimicrobial therapy guidelines are based on indirect modelling from blood antimicrobial levels. However, studies both in humans and animals have shown the fallacy of this concept in various tissues...
December 2010: International Journal of Antimicrobial Agents
S K Chang, J L Davis, C N Cheng, R H Shien, M K Hsieh, B W Koh, C C Chou
Florfenicol (Ff) is a synthetic antibiotic with a broad antibacterial spectrum and high therapeutic effectiveness that was specifically developed for veterinary use. In the present study, tissue residual levels and the pharmacokinetics of Ff after oral administration of 30 mg/kg to Leghorn and Taiwan Native chicken were studied. Furthermore, differential pharmacokinetics between leg and breast muscles were compared using samples collected from an optimized microdialysis model designed for avian species. Significant differences in C(max) were detected between the plasma and muscle microdialysates, and between the breast and leg microdialysates of the Leghorn chickens by noncompartmental pharmacokinetic analysis...
October 2010: Journal of Veterinary Pharmacology and Therapeutics
Inge R H M Konings, Stefan Sleijfer, Ron H J Mathijssen, Peter de Bruijn, Inge M Ghobadi Moghaddam-Helmantel, Linda M van Dam, Erik A C Wiemer, Jaap Verweij, Walter J Loos
PURPOSE: Response to anticancer therapy is believed to be directly related to the concentration of the anticancer drug in the tumor itself. Assessment of intra-tumor drug pharmacokinetics can be helpful to gain more insight into mechanisms involved in the (in)sensitivity of tumors to anticancer therapy. We explored the pharmacokinetics of 5-fluorouracil in both plasma and tumor tissue during a 5-day continuous infusion of 5-fluorouracil in patients with cancer. Sampling for measurement of 5-fluorouracil in tumor tissue was performed using microdialysis...
May 2011: Cancer Chemotherapy and Pharmacology
Yu Zhang, Xiaoli Wang, Xia Lin, Xiaoliang Liu, Bin Tian, Xing Tang
The aim of the present work is to develop high azithromycin loading powders for inhalation with optimal physical and aerodynamic properties, and to test their in vivo potential in rats. A five-level three-factorial central composite rotatable design was used for conducting the experiments. The optimized powder, which had a better flowability, an aerodynamic diameter of 3.82 microm and an in vitro deposition of 51%, was obtained under selected spray-drying conditions. The content of the powder was high (59.2%), which met the requirement for high drug loading...
August 16, 2010: International Journal of Pharmaceutics
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