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https://www.readbyqxmd.com/read/29691845/establishment-of-a-new-fish-cell-line-from-the-caudal-fin-of-golden-pompano-trachinotus-ovatus-and-its-susceptibility-to-iridovirus
#1
S Wei, Y Yu, Q Qin
A new cell line derived from the caudal fin of golden pompano Trachinotus ovatus (TOCF) was successfully established and characterized. TOCF cells grew well at 28° C in L-15 medium supplemented with 10% foetal bovine serum (FBS). The cell line has been subcultured in more than 100 passages. Molecular characterization of 18S ribosomal (r)RNA and cytochrome oxidase subunit 1 (COI) confirmed that the TOCF cells were derived indeed from T. ovatus. TOCF cells have a modal chromosome number of 54. It was further showed that TOCF cells were transfected successfully with pEGFP-N3 and pDsRED-N1 plasmid, suggesting that TOCF cells could be used to research gene functions in vitro...
April 24, 2018: Journal of Fish Biology
https://www.readbyqxmd.com/read/29691550/-effect-of-ball-attachments-with-different-materials-on-retention-of-mandibular-implant-supported-overdenture
#2
Wen-Jun She, Jia-Jing He, Yun-Jia Liu, Ting Jiao
PURPOSE: To investigate the effect of ball attachments with different materials on retention of mandibular implant supported overdenture. METHODS: The experimental groups were divided into group A (titanium ball / titanium cap group) and group B (titanium ball / golden cap group), each group had 3 samples. A standard mandibular edentulous model was made, and the implants (4.8*10 RN SP) were embedded in the bilateral canine region. Mandibular complete dentures were made routinely and the attachments were attached...
December 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29691368/a-novel-sigb-q225p-mutation-in-staphylococcus-aureus-retains-virulence-but-promotes-biofilm-formation
#3
Hui Liu, Weilong Shang, Zhen Hu, Ying Zheng, Jizhen Yuan, Qiwen Hu, Huagang Peng, Xinyu Cai, Li Tan, Shu Li, Junmin Zhu, Ming Li, Xiaomei Hu, Renjie Zhou, Xiancai Rao, Yi Yang
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases that burden human health worldwide. The stress response regulon called sigma factor B (SigB) is a well-characterized global regulator that is involved in the regulation of S. aureus virulence, pigmentation, and biofilm formation. However, the regulatory network upon SigB in S. aureus is incompletely described. Here, we identified a novel substitution mutation, SigB(Q225P), which contributed the nonpigmented phenotype of S...
April 25, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29690461/a-new-species-of-leptolalax-anura-megophryidae-from-son-tra-peninsula-central-vietnam
#4
Luan Thanh Nguyen, Nikolay A Jr Poyarkov, Dzung Trung Le, Ba Dinh Vo, Hoa Thi Phan, Tang van Duong, Robert W Murphy, Sang Ngoc Nguyen
Morphological, acoustic and molecular analyses result in the description of Leptolalax rowleyae sp. nov., a new species of frog in the Megophryidae, belonging to the L. applebyi Rowley Cao species group from central Vietnam. It differs from its congeners by a combination of the following morphological attributes: (1) adult SVL 23.4-25.4 mm in males and 27-27.8 mm in females; (2) presence of distinct dark/brown dorsolateral markings, including black spots on flanks; (3) pinkish milk-white to light brown chest and belly with numerous white speckles; (4) tympanum distinct; (5) absence of webbing or lateral dermal fringes on fingers and toes; (6) pectoral glands comparatively small (3...
February 28, 2018: Zootaxa
https://www.readbyqxmd.com/read/29690255/validity-of-psammoperca-datnioides-richardson-1848-and-redescriptions-of-p-waigiensis-cuvier-in-cuvier-valenciennes-1828-and-hypopterus-macropterus-g%C3%A3-nther-1859-in-the-family-latidae-perciformes-from-the-indo-west-pacific
#5
Yukio Iwatsuki, Stephen J Newman, Fumiya Tanaka, Barry C Russell
Psammoperca datnioides Richardson 1848, long considered a synonym of P. waigiensis (Cuvier in Cuvier Valenciennes 1828), is redescribed as a valid species of Psammoperca Richardson 1848. The species is likely to be endemic to Australia, where it was formerly considered to be P. waigiensis, but differs from P. waigiensis in having the following characters: more slender body (mean depth 28.9% of SL vs. mean depth 36.7% of SL in P.  waigiensis), pored lateral-line scales 49-54 (vs. 46-48 in P. waigiensis), scale rows above and below lateral line 6½ / 10½-11½ (vs 4½ / 9½-10½ in P...
March 29, 2018: Zootaxa
https://www.readbyqxmd.com/read/29688743/serial-culture-is-critical-for-in-vitro-development-of-parthenogenetic-embryos-in-the-golden-syrian-hamster
#6
Guangbin Zhou, Hengxi Wei, Xinrong Wang, Min Yang, Thomas D Bunch, Irina A Polejaeva, Kenneth L White, Zhongde Wang, Qinggang Meng
Unlike oocytes of many other mammalian species, parthenogenetically activated hamster oocytes have not been reported to develop beyond the two-cell stage. This study investigated the in vitro development into blastocysts of parthenogenetic embryos of Golden Syrian hamsters. We observed that hamster oocytes could easily be artificially activated (AA) by treatment with ionomycin plus 6-dimethylaminopurine + cycloheximide + cytochalasin B as assessed by embryo cleavage in HECM-9 (63.15%) or HECM-10 (63.82%). None of the cleaved embryos developed beyond the two-cell stage when cultured in either of the two media...
April 24, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29688158/prospective-comparison-of-the-diagnostic-accuracy-of-mr-imaging-versus-ct-for-acute-appendicitis
#7
Michael D Repplinger, Perry J Pickhardt, Jessica B Robbins, Douglas R Kitchin, Tim J Ziemlewicz, Scott J Hetzel, Sean K Golden, John B Harringa, Scott B Reeder
Purpose To compare the accuracy of magnetic resonance (MR) imaging with that of computed tomography (CT) for the diagnosis of acute appendicitis in emergency department (ED) patients. Materials and Methods This was an institutional review board-approved, prospective, observational study of ED patients at an academic medical center (February 2012 to August 2014). Eligible patients were nonpregnant and 12- year-old or older patients in whom a CT study had been ordered for evaluation for appendicitis. After informed consent was obtained, CT and MR imaging (with non-contrast material-enhanced, diffusion-weighted, and intravenous contrast-enhanced sequences) were performed in tandem, and the images were subsequently retrospectively interpreted in random order by three abdominal radiologists who were blinded to the patients' clinical outcomes...
April 24, 2018: Radiology
https://www.readbyqxmd.com/read/29687961/human-leukocyte-antigens-antibodies-after-lung-transplantation-primary-results-of-the-halt-study
#8
Ramsey R Hachem, Malek Kamoun, Marie M Budev, Medhat Askar, Vivek N Ahya, James C Lee, Deborah J Levine, Marilyn S Pollack, Gundeep S Dhillon, David Weill, Kenneth B Schechtman, Lorriana E Leard, Jeffrey A Golden, LeeAnn Baxter-Lowe, Thalachallour Mohanakumar, Dolly B Tyan, Roger D Yusen
Donor-specific antibodies (DSA) to mismatched human leukocyte antigens (HLA) are associated with worse outcomes after lung transplantation. To determine the incidence and characteristics of DSA early after lung transplantation, we conducted a prospective multi-center observational study that used standardized treatment and testing protocols. Among 119 transplant recipients, 43 (36%) developed DSA: 6 (14%) developed DSA only to class I HLA, 23 (53%) developed DSA only to class II HLA, and 14 (33%) developed DSA to both class I and class II HLA...
April 24, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29686632/parenting-stress-and-resilience-in-parents-of-children-with-autism-spectrum-disorder-asd-in-southeast-asia-a-systematic-review
#9
Kartini Ilias, Kim Cornish, Auretta S Kummar, Miriam Sang-Ah Park, Karen J Golden
Background: This paper aimed to review the literature on the factors associated with parenting stress and resilience among parents of children with autism spectrum disorder (ASD) in the South East Asia (SEA) region. Methods: An extensive search of articles in multiple online databases (PsycNET, ProQuest, PudMed, EMBASE, CINAHL, Web of Science, and Google Scholar) resulted in 28 papers that met the inclusion criteria (i.e., conducted in the SEA region, specific to ASD only, published in a peer-reviewed journal, full text in English)...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/29685553/using-a-new-device-to-prevent-pulmonary-embolisms-during-pharmacomechanical-thrombectomy
#10
E Calleja, J J Ciampi, J J Puche, C Lanciego
We have used a new device to prevent pulmonary embolism during pharmacomechanical thrombectomy in a 25-year-old postpartum woman. The Angel® catheter (BiO2 Medical Inc., Golden, CO, USA) is a temporary device that combines the functions of a vena cava filter (VCF) with those of a triple-lumen central venous catheter. Normally, a VCF is implanted to prevent pulmonary embolism during pharmacomechanical thrombectomy. However, the complications associated with VCFs increase with time, not all of the filter can always be retrieved, and there can be iatrogenic effects both during implantation and retrieval...
April 20, 2018: Radiología
https://www.readbyqxmd.com/read/29684526/targeted-gene-silencing-of-braf-to-interrupt-braf-mek-erk-pathway-synergized-photothermal-therapeutics-for-melanoma-using-a-novel-fa-gnr-sibraf-nanosystem
#11
Yujuan Zhang, Xuelin Zhan, Shanshan Peng, Ying Cai, Yu Shrike Zhang, Yanling Liu, Zhigang Wang, Yanrong Yu, Yifan Wang, Qiaofa Shi, Xiaoping Zeng, Keng Yuan, Nanjin Zhou, Rakesh Joshi, Meng Zhang, Zhuxu Zhang, Weiping Min
Melanoma is significantly associated with mutant BRAF gene, a suitable target for siRNA-based anti-melanoma therapy. However, a tumor-specific delivery system is a major hurdle for clinical applications. Here, we developed a novel nano-carrier, FA-GNR-siBRAF for safe topical application, which consists of folic acid (FA) as the tumor-targeting moiety, golden nanorods (GNR) providing photothermal capability to kill tumor cells under laser irradiation, and siRNA specifically silencing BRAF (siBRAF). The in vitro and in vivo results revealed that FA-GNR-siBRAF displayed high transfection rates, and subsequently induced remarkable gene knockdown of BRAF, resulting in suppression of melanoma growth due to the interruption of the MEK/ERK pathway...
April 20, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29683110/a-new-species-of-the-genus-theloderma-tschudi-1838-amphibia-anura-rhacophoridae-from-tay-nguyen-plateau-central-vietnam
#12
Nikolay A Poyarkov, Ivan I Kropachev, Svetlana S Gogoleva, Nikolai L Orlov
A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloderma auratum sp. nov., is distinguishable from its congeners and other small rhacophorid species based on a combination of the following morphological attributes: (1) bony ridges on head absent; (2) smooth skin completely lacking calcified warts or asperities; (3) pointed elongated tapering snout; (4) vocal opening in males absent; (5) vomerine teeth absent; (6) males of small body size (SVL 21...
April 20, 2018: Zoological Research
https://www.readbyqxmd.com/read/29682792/rapid-assessment-of-pulmonary-gas-transport-with-hyperpolarized-129xe-mri-using-a-3d-radial-double-golden-means-acquisition-with-variable-flip-angles
#13
Kai Ruppert, Faraz Amzajerdian, Hooman Hamedani, Yi Xin, Luis Loza, Tahmina Achekzai, Ian F Duncan, Harrilla Profka, Sarmad Siddiqui, Mehrdad Pourfathi, Maurizio F Cereda, Stephen Kadlecek, Rahim R Rizi
PURPOSE: To demonstrate the feasibility of using a 3D radial double golden-means acquisition with variable flip angles to monitor pulmonary gas transport in a single breath hold with hyperpolarized xenon-129 MRI. METHODS: Hyperpolarized xenon-129 MRI scans with interleaved gas-phase and dissolved-phase excitations were performed using a 3D radial double golden-means acquisition in mechanically ventilated rabbits. The flip angle was either held fixed at 15 ° or 5 °, or it was varied linearly in ascending or descending order between 5 ° and 15 ° over a sampling interval of 1000 spokes...
April 22, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29682783/optimized-respiratory-resolved-motion-compensated-3d-cartesian-coronary-mr-angiography
#14
Teresa Correia, Giulia Ginami, Gastão Cruz, Radhouene Neji, Imran Rashid, René M Botnar, Claudia Prieto
PURPOSE: To develop a robust and efficient reconstruction framework that provides high-quality motion-compensated respiratory-resolved images from free-breathing 3D whole-heart Cartesian coronary magnetic resonance angiography (CMRA) acquisitions. METHODS: Recently, XD-GRASP (eXtra-Dimensional Golden-angle RAdial Sparse Parallel MRI) was proposed to achieve 100% scan efficiency and provide respiratory-resolved 3D radial CMRA images by exploiting sparsity in the respiratory dimension...
April 22, 2018: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29682288/green-infrastructure-and-its-catchment-scale-effects-an-emerging-science
#15
Heather E Golden, Nahal Hoghooghi
Urbanizing environments alter the hydrological cycle by redirecting stream networks for stormwater and wastewater transmission and increasing impermeable surfaces. These changes thereby accelerate the runoff of water and its constituents following precipitation events, alter evapotranspiration processes, and indirectly modify surface precipitation patterns. Green infrastructure, or low-impact development (LID), can be used as a standalone practice or in concert with gray infrastructure (traditional stormwater management approaches) for cost-efficient, decentralized stormwater management...
2018: WIREs. Water
https://www.readbyqxmd.com/read/29681858/aqueous-extract-of-black-maca-prevents-metabolism-disorder-via-regulating-the-glycolysis-gluconeogenesis-tca-cycle-and-ppar%C3%AE-signaling-activation-in-golden-hamsters-fed-a-high-fat-high-fructose-diet
#16
Wenting Wan, Hongxiang Li, Jiamei Xiang, Fan Yi, Lijia Xu, Baoping Jiang, Peigen Xiao
Maca ( Lepidium meyenii Walpers) has been used as a dietary supplement and ethnomedicine for centuries. Recently, maca has become a high profile functional food worldwide because of its multiple biological activities. This study is the first explorative research to investigate the prevention and amelioration capacity of the aqueous extract of black maca (AEM) on high-fat, high-fructose diet (HFD)-induced metabolism disorder in golden hamsters and to identify the potential mechanisms involved in these effects...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29681686/estimating-restorable-wetland-water-storage-at-landscape-scales
#17
Charles Nathan Jones, Grey R Evenson, Daniel L McLaughlin, Melanie K Vanderhoof, Megan W Lang, Greg W McCarty, Heather E Golden, Charles R Lane, Laurie C Alexander
Globally, hydrologic modifications such as ditching and subsurface drainage have significantly reduced wetland water storage capacity (i.e., volume of surface water a wetland can retain) and consequent wetland functions. While wetland area has been well documented across many landscapes and used to guide restoration efforts, few studies have directly quantified the associated wetland storage capacity. Here, we present a novel raster-based approach to quantify both contemporary and potential (i.e., restorable) storage capacities of individual depressional basins across landscapes...
2018: Hydrological Processes
https://www.readbyqxmd.com/read/29680684/fungalbraid-a-goldenbraid-based-modular-cloning-platform-for-the-assembly-and-exchange-of-dna-elements-tailored-to-fungal-synthetic-biology
#18
Miguel Hernanz-Koers, Mónica Gandía, Sandra Garrigues, Paloma Manzanares, Lynne Yenush, Diego Orzaez, Jose F Marcos
Current challenges in the study and biotechnological exploitation of filamentous fungi are the optimization of DNA cloning and fungal genetic transformation beyond model fungi, the open exchange of ready-to-use and standardized genetic elements among the research community, and the availability of universal synthetic biology tools and rules. The GoldenBraid (GB) cloning framework is a Golden Gate-based DNA cloning system developed for plant synthetic biology through Agrobacterium tumefaciens-mediated genetic transformation (ATMT)...
April 19, 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29678693/disseminators-or-silencers-the-effect-of-information-diffusion-intensity-on-cooperation-in-public-goods-game
#19
Tong Chen, Zhenghong Wu, Le Wang
In the Network Era, with the emergence of various instant communication tools, the public are easier to be disseminators than ever. But we have often heard that silence is golden. Inspired by this fact, we investigate that whether the existence of disseminators is beneficial to promote cooperation in the provision of public culture services. To answer this interesting question, we classify disseminators as neutral disseminators, positive disseminators and negative disseminators. And four models are set up, namely, neutral disseminator model (MBD), positive disseminator model (MPD), negative disseminator model (MND) and silencer model (MS)...
April 17, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29678402/golden-jackals-canis-aureus-as-hosts-for-ticks-and-tick-borne-pathogens-in-serbia
#20
Ratko Sukara, Dimosthenis Chochlakis, Duško Ćirović, Aleksandra Penezić, Darko Mihaljica, Sanja Ćakić, Miroslav Valčić, Yannis Tselentis, Anna Psaroulaki, Snežana Tomanović
The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia...
April 10, 2018: Ticks and Tick-borne Diseases
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