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Beta cells AND GSK

Xia Yang, Jian-Ning Lv, Hui Li, Bo Jiao, Qiu-Hong Zhang, Yong Zhang, Jie Zhang, Yan-Qin Liu, Ming Zhang, Hu Shan, Jin-Zhao Zhang, Run-Miao Wu, Ya-Li Li
OBJECTIVES: Asthma is a chronic inflammatory, heterogeneous airway disease affecting millions of people around the world. Curcumin has been found to have anti-inflammatory and antifibrosis effects. Researchers reported that curcumin regulated Wnt/b-catenin signaling in lots of cells. However, whether curcumin regulates the levels of Wnt/ b-Catenin signaling in lung tissues and DCs (dendritic cells) remains unclear. In this study, we assessed the effects of curcumin on DCs and asthma. METHODS: C57BL/6 mice immunized with OVA (ovalbumin) were challenged thrice with an aerosol of OVA every second day for 8 days...
August 15, 2016: Journal of Asthma: Official Journal of the Association for the Care of Asthma
James A McCubrey, Dariusz Rakus, Agnieszka Gizak, Linda S Steelman, Steve L Abrams, Kvin Lertpiriyapong, Timothy L Fitzgerald, Li V Yang, Giuseppe Montalto, Melchiorre Cervello, Massimo Libra, Ferdinando Nicoletti, Aurora Scalisi, Francesco Torino, Concettina Fenga, Luca M Neri, Sandra Marmiroli, Lucio Cocco, Alberto M Martelli
Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that participates in an array of critical cellular processes. GSK-3 was first characterized as an enzyme that phosphorylated and inactivated glycogen synthase. However, subsequent studies have revealed that this moon-lighting protein is involved in numerous signaling pathways that regulate not only metabolism but also have roles in: apoptosis, cell cycle progression, cell renewal, differentiation, embryogenesis, migration, regulation of gene transcription, stem cell biology and survival...
September 6, 2016: Biochimica et Biophysica Acta
Yi-Fei Qi, Long Wu, Zi-Qian Li, Meng-Ling Wu, Hai-Fang Wang, Ka-Ying Chan, Lin-Lin Lu, Shao-Hui Cai, Hong-Sheng Wang, Jun Du
Nodal is a member of transforming growth factor beta (TGF-β) superfamily. Nodal promotes the self-renewal of human cancer stem cells (CSCs) and triggers carcinogenesis of human cancers via an autocrine manner through Smad2/3 pathway. In our study, generation of Nodal-overexpressed cancer cells was constructed, and the effect of Nodal on the stem cell marker Oct-4 was evaluated by overexpression or blocked Nodal/ALKs signaling pathway in non-small cell lung cancer cells A549 and prostate cancer cells PC3. Functionally, Nodal also increased the proliferation via the β-catenin nuclear translocation...
October 15, 2016: Archives of Biochemistry and Biophysics
Shuping Fu, Li Yang, Hao Hong, Ronghua Zhang
OBJECTIVE: To investigate the effect of icariin on proliferation of bone marrow mesenchymal stem cells (BMSCs) in Sprague-Dawley (SD) rats. METHODS: BMSCs were obtained from SD rat bone marrow with differential time adherent method. Its characteristic was identified through differentiation cell surface antigens and the multi-lineage (osteo/adipo/chondo) differentiation potential. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and 5-Bromo-2-Deoxyuridine (BrdU) incorporation were applied to detect the effect of icariin on BMSCs proliferation...
June 2016: Journal of Traditional Chinese Medicine, Chung i Tsa Chih Ying Wen Pan
C Xiao, C-H Wu, H-Z Hu
OBJECTIVE: LncRNA UCA1 can promote invasion of breast cancer cells. However, the underlying mechanism is not quite clear. In this study, we investigated the regulative effect of UCA1 on the invasion capability of breast cancer cells and its association with the Wnt/β-catenin pathway. MATERIALS AND METHODS: Human breast cancer cell line MDA-MB-231 cells were transfected for UCA1 knockdown using UCA1 si-RNA. Transwell assay was performed to assess cell invasion capability...
July 2016: European Review for Medical and Pharmacological Sciences
Åke Lernmark
The etiology of human leukocyte antigen (HLA)-associated organ-specific autoimmune diseases is incomplete. In type 1 diabetes and celiac disease, the strongest associations are with the HLA-DR3-DQ2 and DR4-DQ8 haplotypes, whereas the DQB1*06:02 allele has a strong negative association. In contrast, narcolepsy, especially as recently triggered by the Pandemrix(®) H1N1 vaccine (GlaxoKlineSmith (GSK), Brentford, Middlesex, UK), did not seem to develop without at least one copy of the latter allele. The overall hypothesis is that the role of these different HLA haplotypes, especially in Finland and Sweden, is related to the immune response to infectious agents that are common in these two populations...
July 2016: Pediatric Diabetes
Yan Yang, Yan Liu, Jun-Chuang He, Jian-Ming Wang, Peter Schemmer, Chao-Qun Ma, Ya-Wei Qian, Wei Yao, Jian Zhang, Wei-Peng Qi, Yang Fu, Wei Feng, Tao Yang
Cholangiocarcinoma (CCA) invasion and metastasis are the primary causes of poor survival rates in patients. The epithelial-mesenchymal transition (EMT) is a crucial step in cancer invasion and metastasis. However, it is still unclear of the molecular mechanism. In this study, the expression of 14-3-3ζ and atypical protein kinase C-ι (aPKC-ι) was further detected in CCA tissues and cell lines. Meanwhile, we established the EMT model of CCA cells and investigated 14-3-3ζ and aPKC-ι co-regulatory effect on the EMT in vitro and in vivo...
July 8, 2016: Oncotarget
İlhan Elmaci, Meric A Altinoz
Pancreatic cancer (PC) and glioblastoma multiforme (GBM) are among the human cancers with worst prognosis which require an urgent need for efficient therapies. Here, we propose to apply to treat both malignancies with a triple combination of drugs, which are already in use for different indications. Recent studies demonstrated a considerable link between risk of PC and diabetes. In experimental models, anti-diabetogenic agents suppress growth of PC, including metformin (M), pioglitazone (P) and lithium (L)...
October 2016: Biochemical Genetics
Zhao-Xiang Ren, Ya-Fei Zhao, Ting Cao, Xue-Chu Zhen
AIM: It is general believed that mitochondrial dysfunction and oxidative stress play critical roles in the pathology of Parkinson's disease (PD). Dihydromyricetin (DHM), a natural flavonoid extracted from Ampelopsis grossedentata, has recently been found to elicit potent anti-oxidative effects. In the present study, we explored the role of DHM in protecting dopaminergic neurons. METHODS: Male C57BL/6 mice were intraperitoneally injected with 1-methyl4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) for 7 d to induce PD...
July 4, 2016: Acta Pharmacologica Sinica
Yavuz Aras, Mine Erguven, Esin Aktas, Nuray Yazihan, Ayhan Bilir
OBJECTIVES: Glioblastoma (GBM), the most common primary tumour of the central nervous system, is characterised by a high malignancy and poor prognosis. The aims of this study were to investigate whether the combination of imatinib mesylate (IM) and lithium chloride (LiCl) exhibited a synergistic effect in treatment and to determine whether midkine (MK) affected the fate of this treatment in vitro. METHODS: Monolayer and spheroid cultures of the T98G human GBM cell line were treated with an IM and LiCl combination for 72 h...
September 2016: Neurological Research
Shabbir Khan Afridi, Meha Fatima Aftab, Munazza Murtaza, Safina Ghaffar, Aneela Karim, Uzma Rasool Mughal, Khalid Mohammed Khan, Rizwana Sanaullah Waraich
Glycotoxins/Advanced glycation end products (AGEs) have implications in development of diabetes and related diseases. In the present study we deciphered the mechanisms of action of URM-II-81, a new derivative of isatin, in alleviation of insulin resistance in human hepatocytes and murine adipocytes. URM-II-81 reduced AGEs formation and receptor for advanced glycation end products (RAGE) expression in both cell types. We also observed suppression of methylglyoxal (MGO) mediated ROS production and deactivation of PKC-α...
August 5, 2016: Biochemical and Biophysical Research Communications
Yan-Fang Xian, Siu-Po Ip, Qing-Qiu Mao, Zhi-Xiu Lin
Beta-amyloid (Aβ) accumulation, one of the most important pathogenic traits of Alzheimer's disease (AD), has been reported to induce neurotoxicity in vitro as well as in vivo. Honokiol, isolated from the bark of Magnolia officinalis, has neuroprotective effects in different models of AD in vivo and in vitro. However, the exact mechanism for its neuroprotective effect is not well understood. The present study aimed to investigate the molecular mechanisms underlying the protective action of honokiol against Aβ1-42-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells...
July 2016: Neurochemistry International
Cristian Fabián Layton Tovar, Hugo Mendieta Zerón
Acute lymphoblastic leukemia (ALL) is a malignant disease characterized by an uncontrolled proliferation of immature lymphoid cells. ALL is the most common hematologic malignancy in early childhood, and it reaches peak incidence between the ages of 2 and 3 years. The prognosis of ALL is associated with aberrant gene expression, in addition to the presence of numerical or structural chromosomal alterations, age, race, and immunophenotype. The Relapse rate with regard to pharmacological treatment rises in childhood; thus, the expression of biomarkers associated with the activation of cell signaling pathways is crucial to establish the disease prognosis...
June 2016: Indian Journal of Hematology & Blood Transfusion
Limin Zhang, Luan Cen, Shaogang Qu, Lei Wei, Mingshu Mo, Junmin Feng, Congcong Sun, Yousheng Xiao, Qin Luo, Shaomin Li, Xinling Yang, Pingyi Xu
Parkinson's disease (PD) is characterized by progressive degeneration of dopaminergic (DA) neurons in the substantial nigra pars compacta. Increasing evidence showed that Wnt/β-catenin pathway and the orphan nuclear receptor Nurr1 play crucial roles in the survival and functional maintenance of DA neurons in the midbrain and GSK-3β antagonists LiCl and SB216763 were used to activate Wnt/β-catenin pathway experimentally. However, the detail mechanism underlying the neuroprotection against apoptosis on DA neuron is still unclear and the interaction between Wnt/β-catenin and Nurr1 remains undisclosed...
2016: PloS One
Eduardo H Sanchez-Mendoza, Jana Schlechter, Dirk M Hermann, Thorsten R Doeppner
Stem cell research depends on extensive in vitro research. Poly-D-lysine (PDL) and polyornithine (PornT) are chemically synthesized amino acid chains promoting cell adhesion to solid substrates. Although, PDL and PornT are extensively used, there is no common agreement regarding the most optimal substance and its concentration. We therefore aimed at testing the effect of increasing concentrations (10, 50, and 100 μg/ml) for each compound and their corresponding mixtures (5+5 and 10+10 μg/ml) on the differentiation patterns of subventricular zone derived neurospheres...
2016: Frontiers in Cellular Neuroscience
Ying-nian Shen, Wei-lin Hu, Zheng-ping Chen, Li Cai, Yong-sheng Li
Aortic valve calcification (AVC) is a pathological process correlated with multiple disease causes and actively regulated by cardiac valve cells. In this study, porcine aortic valve myofibroblasts cultured in vitro were treated with 50 μg z L(-1) of pathological factor tumor necrosis factor α (TNF-α). Tanshinone II A (TSN) with the concentration of 50 mg x L(-1) and TNF-α were combined in incubating cells for 72 h (3 d) and 120 h (5 d). The Western blotting and Real-time PCR were adopted to detect the changes in smooth muscle α actin (α-SMA), bone morphogenetic protein 2 ( BMP2), alkaline phosphatase (ALP) in cells, and expressions of key effect proteins GSK-3β and β-catenin on Wnt/β-catenin signal pathway...
September 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Ming-Yao Gu, Joonki Kim, Hyun Ok Yang
Justicidin A is a structurally defined arylnaphthalide lignan, which has been shown anti-cancer activity; however, the neuroprotective effect of justicidin A is still untested. In this study, we investigated the action of justicidin A on amyloid beta (Aβ)25-35-induced neuronal cell death via inhibition of the hyperphosphorylation of tau and induction of autophagy in SH-SY5Y cells. Pretreatment with justicidin A significantly elevated cell viability in cells treated with Aβ25-35. Western blot data demonstrated that justicidin A inhibited the Aβ25-35-induced up-regulation the levels of hyperphosphorylation of tau in SH-SY5Y cells...
June 2016: Neurochemical Research
Pablo Fernández-Pernas, Juan Fafián-Labora, Iván Lesende-Rodriguez, Jesús Mateos, Alexandre De la Fuente, Isaac Fuentes, Javier De Toro Santos, Fco Blanco García, María C Arufe
Our group focuses on the study of mesenchymal stem cells (MSCs) from human umbilical cord stroma or Warthońs jelly and their directed differentiation toward chondrocyte-like cells capable of regenerating damaged cartilage when transplanted into an injured joint. This study aimed to determine whether lactogenic hormone prolactin (PRL) or 3, 3', 5-triiodo-L-thyronine (T3), the active thyroid hormone, modulates chondrogenesis in our in vitro model of directed chondrogenic differentiation, and whether Wnt signalling is involved in this modulation...
September 2016: Journal of Cellular Biochemistry
Changjiang Ying, Xiaoyan Zhou, Zhenzhen Chang, Hongwei Ling, Xingbo Cheng, Wei Li
Blood glucose fluctuation is associated with diabetic nephropathy. However, the mechanism by which blood glucose fluctuation accelerates renal injury is not fully understood. The aim of the present study was to assess the effects of blood glucose fluctuation on diabetic nephropathy in rats and investigate its underlying mechanism. Diabetes in the rats was induced by a high sugar, high-fat diet, and a single dose of STZ (35 mg/kg)-injected intraperitoneally. Unstable blood sugar models were induced by subcutaneous insulin injection and intravenous glucose injection alternately...
July 2016: Endocrine
Lin Yan, Guangyong Shi
OBJECTIVE: This study explored how interferon alpha (IFN-α) influenced liver cancer SMCC-7721 cell via PI3K/Akt signaling pathway. METHODS: The effect of IFN-α on the viability of SMCC-7721 cell was measured by MTT assay. The effect of IFN-α on SMCC-7721 cell cycle was measured by flow cytometry. The activation of PI3K/Akt signaling pathway and downstream target genes expression level of glycogen synthase kinase 3 beta (GSK-3β), and cell cycle protein (Cyclin D1), p21, C-myc, Survivin were assayed by Western blot...
September 22, 2015: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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