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https://www.readbyqxmd.com/read/28102755/anionic-lipids-and-the-maintenance-of-membrane-electrostatics-in-eukaryotes
#1
Matthieu Pierre Platre, Yvon Jaillais
A wide range of signaling processes occurs at the cell surface through the reversible association of proteins from the cytosol to the plasma membrane. Some low abundant lipids are enriched at the membrane of specific compartments and thereby contribute to the identity of cell organelles by acting as biochemical landmarks. Lipids also influence membrane biophysical properties, which emerge as an important feature in specifying cellular territories. Such parameters are crucial for signal transduction and include lipid packing, membrane curvature and electrostatics...
January 19, 2017: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/28096331/one-step-design-of-a-stable-variant-of-the-malaria-invasion-protein-rh5-for-use-as-a-vaccine-immunogen
#2
Ivan Campeotto, Adi Goldenzweig, Jack Davey, Lea Barfod, Jennifer M Marshall, Sarah E Silk, Katherine E Wright, Simon J Draper, Matthew K Higgins, Sarel J Fleishman
Many promising vaccine candidates from pathogenic viruses, bacteria, and parasites are unstable and cannot be produced cheaply for clinical use. For instance, Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is essential for erythrocyte invasion, is highly conserved among field isolates, and elicits antibodies that neutralize in vitro and protect in an animal model, making it a leading malaria vaccine candidate. However, functional RH5 is only expressible in eukaryotic systems and exhibits moderate temperature tolerance, limiting its usefulness in hot and low-income countries where malaria prevails...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28073977/the-effects-of-a-modified-hot-water-treatment-on-anastrepha-ludens-diptera-tephritidae-infested-mango
#3
Emilio Hernández, Marysol Aceituno-Medina, Jorge Toledo, Bigail Bravo, José Caro-Corrales, Pablo Montoya, Robert Mangan
The Mexican fruit fly, Anastrepha ludens (Loew), is a quarantine pest in mango (Mangifera indica L.) that can be controlled by using a hot water treatment (HWT). This treatment is normally followed by a 30-min hydrocooling (HYC) process that reduces the negative effects that the treatment has on fruit quality. However, if hot water-treated fruits are immediately immersed in water at 21 °C, the survival rate of third-instar A. ludens may be increased. The current approved treatment protocol states that if HYC is used, then treated fruit should undergo an additional 10-min HWT or on platform for 30 min before HYC...
January 10, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28071844/a-vesicle-to-worm-transition-provides-a-new-high-temperature-oil-thickening-mechanism
#4
Matthew J Derry, Oleksandr O Mykhaylyk, Steven P Armes
Diblock copolymer vesicles are prepared via RAFT dispersion polymerization directly in mineral oil. Such vesicles undergo a vesicle-to-worm transition on heating to 150 °C, as judged by TEM and SAXS. Variable-temperature (1) H NMR spectroscopy indicates that this transition is the result of surface plasticization of the membrane-forming block by hot solvent, effectively increasing the volume fraction of the stabilizer block and so reducing the packing parameter for the copolymer chains. The rheological behavior of a 10 % w/w copolymer dispersion in mineral oil is strongly temperature-dependent: the storage modulus increases by five orders of magnitude on heating above the critical gelation temperature of 135 °C, as the non-interacting vesicles are converted into weakly interacting worms...
January 10, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28058480/everyman-s-prostate-phantom-kiwi-fruit-substitute-for-human-prostates-at-magnetic-resonance-imaging-diffusion-weighted-imaging-and-magnetic-resonance-spectroscopy
#5
Ullrich G Mueller-Lisse, Sophie Murer, Ulrike L Mueller-Lisse, Marissa Kuhn, Juergen Scheidler, Michael Scherr
OBJECTIVES: To apply an easy-to-assemble phantom substitute for human prostates in T2-weighted magnetic resonance imaging (T2WI), diffusion-weighted imaging (DWI) and 3D magnetic resonance spectroscopy (MRS). METHODS: Kiwi fruit were fixed with gel hot and cold compress packs on two plastic nursery pots, separated by a plastic plate, and submerged in tap water inside a 1-L open-spout plastic watering can for T2WI (TR/TE 7500/101 ms), DWI (5500/61 ms, ADC b50-800 s/mm(2) map) and MRS (940/145 ms) at 3...
January 5, 2017: European Radiology
https://www.readbyqxmd.com/read/28051342/effects-of-thermal-agent-and-physical-activity-on-muscle-tendon-stiffness-and-effects-combined-with-static-stretching
#6
Kosuke Fujita, Masatoshi Nakamura, Hiroki Umegaki, Takuya Kobayashi, Satoru Nishishita, Hiroki Tanaka, Satoko Ibuki, Noriaki Ichihashi
CONTEXT: A recent review or article reported that thermal agents (TA) or physical activity (PA) can increase range of motion (ROM), and that the combination of TA with stretching is superior to performing stretching only. However, since ROM is affected by the participants' psychological factors, it is questionable whether these studies measured the effect of these intervention on muscle flexibility. By measuring muscle stiffness, we attempted to evaluate the effect these intervention on muscle flexibility...
January 4, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/27942126/availability-usage-and-factors-affecting-usage-of-electrophysical-agents-by-physical-therapists-a-regional-cross-sectional-survey
#7
Yuichi Abe, Ah-Cheng Goh, Kei Miyoshi
[Purpose] The aim of this study was to investigate the availability, usage, and factors affecting usage of electrophysical agents by physical therapists in Nagano Prefecture, Japan. [Subjects and Methods] Questionnaires were sent to all 1,571 physical therapists working in 245 institutions within Nagano Prefecture. A total of 1,110 questionnaires were returned, out of which 1,099 (70%) questionnaires containing valid responses were analyzed. Frequencies and percentages were calculated for 22 modalities with regards to availability, usage, rate of usage, and confidence level in usage...
November 2016: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/27925258/ohmic-heating-of-an-electrically-conductive-food-package
#8
Kanyawee Kanogchaipramot, Kullanart Tongkhao, Tanaboon Sajjaanantakul, Pitiya Kamonpatana
Ohmic heating through an electrically conductive food package is a new approach to heat the food and its package as a whole after packing to avoid post-process contamination and to serve consumer needs for convenience. This process has been successfully completed using polymer film integrated with an electrically conductive film to form a conductive package. Orange juice packed in the conductive package surrounded with a conductive medium was pasteurized in an ohmic heater. A mathematical model was developed to simulate the temperature distribution within the package and its surroundings...
December 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27898256/comparative-study-of-the-physiotherapeutic-and-drug-protocol-and-low-level-laser-irradiation-in-the-treatment-of-pain-associated-with-temporomandibular-dysfunction
#9
Marcos Fernando Xisto Braga Cavalcanti, Uoston Holder Silva, Ernesto Cesar Pinto Leal-Junior, Rodrigo A B Lopes-Martins, Rodrigo Labat Marcos, Rodney Capp Pallotta, Francesca Diomede, Oriana Trubiani, Natalia De Isla, Lucio Frigo
BACKGROUND: The temporomandibular joint (TMJ) is a structure of the craniofacial complex affected by neurological diseases. Orthopedic and musculoskeletal changes can also cause temporomandibular disorders (TMD) and pain. Low-level laser (LLL) therapy has been studied in the treatment of temporomandibular jaw (TMJ) dysfunction, and controversial results were obtained. OBJECTIVE: The objective of this work was comparing the physiotherapeutic and drug protocol (PDP) to LLL therapy in the treatment of pain associated with TMD...
December 2016: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/27886641/comparison-of-hot-versus-cold-boning-of-beef-carcasses-on-bacterial-growth-and-the-risk-of-blown-pack-spoilage
#10
Rachael Reid, Séamus Fanning, Paul Whyte, Joe Kerry, Declan Bolton
Primals were prepared from beef Longissimus thoracis et lumborum (LTL), psoas major (PM), quadriceps femoris (QF) and semitendinosus (S) muscles from cold and hot boned carcasses, vacuum-packaged and stored for 42 or 100days at 2°C and 7°C. Storage temperature, carcass or primal surface temperature, pH and aw were monitored. Samples were taken periodically and tested for total viable count mesophilic (TVCm), TVC psychrophilic (TVCp), total Enterobacteriaceae count (TEC), presumptive Pseudomonas spp., lactic acid bacteria (LAB), Clostridium spp...
March 2017: Meat Science
https://www.readbyqxmd.com/read/27870027/hunting-on-a-hot-day-effects-of-temperature-on-interactions-between-african-wild-dogs-and-their-prey
#11
Scott Creel, Nancy M Creel, Andrea M Creel, Bridget M Creel
As global temperatures increase, interactions between species are affected by changes in distribution, abundance and phenology, but also by changes in behavior. The heat dissipation limitation hypothesis suggests that the ability to dissipate heat commonly limits the activity of endotherms, a problem that should be particularly acute for cursorial predators and their prey in equatorial ecosystems. Allometric relationships suggest that heat dissipation should be a stronger constraint for larger species, so that (smaller) predators should be less affected than (larger) prey...
November 2016: Ecology
https://www.readbyqxmd.com/read/27865514/coagulase-negative-staphylococcus-species-in-bulk-milk-prevalence-distribution-and-associated-subgroup-and-species-specific-risk-factors
#12
A De Visscher, S Piepers, F Haesebrouck, K Supré, S De Vliegher
Coagulase-negative staphylococci (CNS) have become the main pathogens causing bovine mastitis in recent years. A huge variation in species distribution among herds has been observed in several studies, emphasizing the need to identify subgroup- and species-specific herd-level factors to improve our understanding of the differences in ecological and epidemiological nature between species. The use of bulk milk samples enables the inclusion of a large(r) number of herds needed to identify herd-level risk factors and increases the likelihood of recovering enough isolates per species needed for conducting subgroup- and, eventually, species-specific analyses at the same time...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27859918/greenhouse-gas-emissions-from-dairy-manure-management-in-a-mediterranean-environment
#13
Justine J Owen, Whendee L Silver
Livestock agriculture is a major source of anthropogenic greenhouse gas (GHG) emissions, with a substantial proportion of emissions derived from manure management. Accurate estimates of emissions related to management practices and climate are needed for identifying the best approaches to minimize, and potentially mitigate, GHG emissions. Current emissions models such as those of the IPCC, however, are based on emissions factors that have not been broadly tested against field-scale measurements due to a lack of data...
November 11, 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27844087/approaching-the-size-limit-of-organometallic-layers-synthesis-and-characterization-of-highly-ordered-silver-thiolate-lamellae-with-ultra-short-chain-lengths
#14
Zichao Ye, Lito P de la Rama, Mikhail Y Efremov, Jian-Min Zuo, Leslie H Allen
Approaching the ultimate limits of material sizes provides a route for designing new functional materials with extraordinary properties. We report the first systematic synthesis and characterization study of a wide range of highly ordered silver alkanethiolate (AgSCnH2n+1 or AgSCn, n = 1-16) aliphatic lamellae. Single crystalline multilayer AgSCn are synthesized by a modified solution reaction method. Hot toluene recrystallization or Ostwald ripening enhances the structural ordering of the lamellar crystals...
November 15, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27827904/an-soi-cmos-based-multi-sensor-mems-chip-for-fluidic-applications
#15
Mohtashim Mansoor, Ibraheem Haneef, Suhail Akhtar, Muhammad Aftab Rafiq, Andrea De Luca, Syed Zeeshan Ali, Florin Udrea
An SOI CMOS multi-sensor MEMS chip, which can simultaneously measure temperature, pressure and flow rate, has been reported. The multi-sensor chip has been designed keeping in view the requirements of researchers interested in experimental fluid dynamics. The chip contains ten thermodiodes (temperature sensors), a piezoresistive-type pressure sensor and nine hot film-based flow rate sensors fabricated within the oxide layer of the SOI wafers. The silicon dioxide layers with embedded sensors are relieved from the substrate as membranes with the help of a single DRIE step after chip fabrication from a commercial CMOS foundry...
November 4, 2016: Sensors
https://www.readbyqxmd.com/read/27816913/laser-acupuncture-treatment-improves-pain-and-functional-status-in-patients-with-subacromial-impingement-syndrome-a-randomized-double-blind-sham-controlled-study
#16
Sibel Kibar, Hatice Ecem Konak, Deniz Evcik, Saime Ay
OBJECTIVE:  The present study aims to determine the effect of laser acupuncture in patients with subacromial impingement syndrome (SAIS). DESIGN:  Randomized, double-blind, sham-controlled study. SETTING:  Physical medicine and rehabilitation outpatient clinic. SUBJECTS:  A total 73 patients with SAIS were randomly assigned into a treatment group (n = 36) and a control group (n = 37). METHODS : The treatment group received laser acupuncture, and the control group received sham laser acupuncture...
November 5, 2016: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/27786467/phase-transition-in-octahydro-1-3-5-7-tetranitro-1-3-5-7-tetrazocine-hmx-under-static-compression-an-application-of-the-first-principles-method-specialized-for-chno-solid-explosives
#17
Lei Zhang, Sheng-Li Jiang, Yi Yu, Yao Long, Han-Yue Zhao, Li-Juan Peng, Jun Chen
The first-principles method is challenged by accurate prediction of van der Waals interactions, which are ubiquitous in nature and crucial for determining the structure of molecules and condensed matter. We have contributed to this by constructing a set of pseudopotentials and pseudoatomic orbital basis specialized for molecular systems consisting of C/H/N/O elements. The reliability of the present method is verified from the interaction energies of 45 kinds of complexes (comparing with CCSD(T)) and the crystalline structures of 23 kinds of typical explosive solids (comparing with experiments)...
November 10, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27700031/engineering-gold-nanoparticles-in-compass-shape-with-broadly-tunable-plasmon-resonances-and-high-performance-sers
#18
Youju Huang, Liwei Dai, Liping Song, Lei Zhang, Yun Rong, Jiawei Zhang, Zhihong Nie, Tao Chen
We present the uniform and high-yield synthesis of a novel gold nanostructure of compass shape composed of a Au sphere at the central and two gradually thinning conical tips at the opposed poles. The Au compass shapes were synthesized through a seed-mediated growth approach employing a binary mixture of cetyltrimethylammonium bromide (CTAB) and sodium oleate (NaOL) as the structure-directing agents. Under the condition of single surfactant (CTAB), the spherical seeds tend to grow into larger spherical Au nanoparticles (NPs); while the spherical seeds favor the formation of Au compass shaped NPs using two mixed surfactants (CTAB/NaOL)...
October 4, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27687894/isolation-of-a-transcription-factor-dreb1a-gene-from-phaseolus-vulgaris-and-computational-insights-into-its-characterization-protein-modeling-docking-and-mutagenesis
#19
Recep Vatansever, Mehmet Emin Uras, Ugur Sen, Ibrahim Ilker Ozyigit, Ertugrul Filiz
A transcription factor DREB1A (dehydration-responsive element-binding 1A) gene was amplified and sequenced from the common bean (Phaseolus vulgaris). PvDREB1A had a 777 base pairs (bp) open reading frame encoding a protein of 225 residues. The protein sequence contained a conserved DNA-binding AP2 domain of about 60 residues and a nuclear localization signal (NLS) at N-terminus site. PvDREB1A demonstrated high homology with other DREB1 members only in AP2 domain and NLS site. The phylogenetic distribution of different DREB members showed three main groups as DREB1-3 and PvDREB1A was a member of DREB1 group...
October 14, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27642600/motor-ingredients-derived-from-a-wearable-sensor-based-virtual-reality-system-for-frozen-shoulder-rehabilitation
#20
Si-Huei Lee, Shih-Ching Yeh, Rai-Chi Chan, Shuya Chen, Geng Yang, Li-Rong Zheng
Objective. This study aims to extract motor ingredients through data mining from wearable sensors in a virtual reality goal-directed shoulder rehabilitation (GDSR) system and to examine their effects toward clinical assessment. Design. A single-group before/after comparison. Setting. Outpatient research hospital. Subjects. 16 patients with frozen shoulder. Interventions. The rehabilitation treatment involved GDSR exercises, hot pack, and interferential therapy. All patients first received hot pack and interferential therapy on the shoulder joints before engaging in the exercises...
2016: BioMed Research International
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