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María Cristina Romero-Rodríguez, Jesús V Jorrín-Novo, María Angeles Castillejo
By using two complementary proteomics, gel-based and gel-free (shotgun) approaches, the protein profiles of the non-orthodox forest tree species Quercus ilex seeds during germination and early seedling growth have been compared. Proteins were extracted from embryo axis, radicle and shoot tissues at different developmental stages. Proteins were subjected to one- and two-dimensional gel electrophoresis. A multivariate analysis (PCA) revealed that SDS-PAGE clearly separated germination (0-24 h post-imbibition), postgermination (72-216 h post-imbibition) and early seedling growth stages (2 weeks post-imbibition)...
November 5, 2018: Journal of Proteomics
Susmita Das, Barsha Majumder, Asok K Biswas
Arsenic is a carcinogenic metalloid, exists in two important oxidation states-arsenate (As-V) and arsenite (As-III). The influence of arsenate with or without silicate on the growth and thiol metabolism in rice (Oryza sativa L. cv. MTU-1010) seedlings were investigated. Arsenate was more toxic for root growth than shoot growth where the root lengths were short, characteristically fragile and root tips turned brown. The multiple comparison analysis using Tukey's HSD (honest significant difference) tests indicated that the rate of arsenate accumulation and its conversion to arsenite by arsenate reductase were significantly increased in all arsenate treated seedlings while in seedlings treated jointly with arsenate and silicate, arsenate accumulation and its conversion to arsenite decreased...
November 8, 2018: Ecotoxicology
Nusrat Jahan, Fazilah Abd Manan, Arsala Mansoor, Mudassir Asrar Zaidi, Muhammad Naeem Shahwani, Muhammad Arshad Javed
Rice production is decreasing by abiotic stresses like heavy metals. In such circumstances, producing food for growing human population is a challenge for plant breeders. Excess of Al3+ in soil has become threat for high yield of rice. Improvement of crop is one of potential solution for high production. The aim of this study was to develop the new method for optimization of Al3+ toxicity tolerance in indica rice at germination stag using two-way ANOVA and Duncan's multiple-range test (DMRT). Seeds of two indica rice cultivars ( Pokkali and Pak Basmati) were exposed in different concentrations (control, 5 mM, 15 mM, and 20 mM) of Al3+ toxicity at pH 4 ±0...
2018: TheScientificWorldJournal
Chao Ni, Dongyi Wang, Yang Tao
Spectroscopy is a powerful non-destructive quantization tool. In this paper, the technology is used to predict the nitrogen content of Masson pine seedling leaves. Masson pine is widely planted in China, and its nitrogen content is an important index for evaluating the vigour of seedings. To establish a better prediction model, an improved 1D convolutional neural network architecture, named the variable weighted convolutional neural network (VWCNN), is proposed in this research. The new model can automatically force the network attention onto the important spectrum wavelengths and is able to improve the generalization ability of the basic 1D-CNN model...
October 15, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Daniel López-López, Ricardo Becerro-de-Bengoa-Vallejo, Marta Elena Losa-Iglesias, Patricia Palomo-López, David Rodríguez-Sanz, Juan Manuel Brandariz-Pereira, César Calvo-Lobo
OBJECTIVE: Foot problems (FP) may be considered to be a prevalent conditions in people but there is limited evidence of their effect on the quality of life (QoL) related to foot health in men and women. The aim of this study was to assess the impact of FPs on both overall foot health and QoL, stratified by gender. DESIGN: A cross sectional study. SETTING: Clinic of Podiatric Medicine and Surgery at University of A Coruña in the city of Ferrol (Spain)...
October 18, 2018: BMJ Open
Fiona R Hay, Rocel Valdez, Jae-Sung Lee, Pompe C Sta Cruz
The length of time that seed lots maintain their viability and vigour in storage has become a trait of interest for rice and other crop and model species. However, different research groups have taken different approaches to measure this trait, including storing seeds under 'natural ageing', accelerated ageing and controlled deterioration test conditions. There has also been a tendency to only use a single germination test result to assess the relative longevity of different genotypes. Here we explain why we have taken a different approach in our GWAS study of seed longevity in Indica rice, and offer suggestions as to how, moving forward, this trait should be assessed to make it possible to improve the longevity / storability of seeds, depending on the target storage environment...
October 16, 2018: Journal of Experimental Botany
Marieke H A van Hulten, Maria-Joāo Paulo, Willem Kruijer, Hetty Blankestijn-De Vries, Brend Kemperman, Frank F M Becker, Jiaming Yang, Kathrin Lauss, Maike E Stam, Fred A van Eeuwijk, Joost J B Keurentjes
Hybrid vigour, or heterosis, has been of tremendous importance in agriculture for the improvement of both crops and livestock. Notwithstanding large efforts to study the phenomenon of heterosis in the last decades, the identification of common molecular mechanisms underlying hybrid vigour remain rare. Here, we conducted a systematic survey of the degree of heterosis in Arabidopsis thaliana hybrids. For this purpose, two overlapping Arabidopsis hybrid populations were generated by crossing a large collection of naturally occurring accessions to two common reference lines...
2018: PloS One
V Benard, B Etain, G Vaiva, C Boudebesse, S Yeim, C Benizri, H Brochard, F Bellivier, P A Geoffroy
BACKGROUND: The poor prognostic of Bipolar disorders (BD) is closely linked to deaths by suicide. Sleep and circadian abnormalities are observed during all phases of BD and are also associated with suicide attempt (SA). In this context, this study sought to identify specific sleep and circadian rhythms markers associated with suicidal attempt in euthymic patients with BD. METHODS: The sample (N = 236) comprised 3 groups: 147 patients with BD including 57 with a history of SA and 90 without (NoSA), and 89 healthy controls (HC)...
September 18, 2018: Journal of Affective Disorders
Zhan Li, Yue Gao, Cheng Lin, Ronghui Pan, Wenguang Ma, Yunye Zheng, Yajing Guan, Jin Hu
The preservation of seed viability and quality in storage is an important trait both for commercial and germplasm usage. To better explore potential mechanisms of tobacco seed deterioration, seed packed in cloth bag (C) and vacuum bag (V) were stored under room temperature (RT) and low temperature (LT, 18°C), and sampled periodically for laboratory testing. Seed stored in low temperature with vacuum bag (LT/V) owned the highest seed vigour after 25 months of storage and in room temperature with cloth bag (RT/C) lost seed vigour and germination ability after 20-month storage...
2018: Conservation Physiology
D V C Almeida, J S Lima, D L Leão, K G Oliveira, R R Santos, S F S Domingues, M S Miranda
SummaryThe aim of this study was to evaluate the effect of incubating semen for different periods (90, 270 or 450 min) with or without Trolox® (100 or 150 µM) on the quality of sperm from Saimiri collinsi. Sperm motility, vigour, and plasma membrane integrity (PMI) were evaluated in both fresh semen and semen incubated for different time periods, i.e. 90, 270 or 450 min of incubation. Supplementation of semen extender with Trolox® 100 µM improved sperm motility, vigour and PMI for up to 270 min of incubation...
August 2018: Zygote: the Biology of Gametes and Early Embryos
Shainy Nhattuketty Krishnan, Anuraj Nayarisseri, Usha Rajamanickam
Cresols are ubiquitous due to industrial production and natural presence. o-cresol (2-methyl phenol) is highly toxic to both fauna and flora. It has been included in the EPA list as one of the priority pollutants. The deleterious effects of pesticides, herbicides, and many other chemical compounds on seed germination are known. However, the effect of o-cresol on seed germination is not known. Therefore, it is of interest to study the effect of o-cresol on germination of 13 different vegetable crop seeds using standard Filter Paper Method...
2018: Bioinformation
Jan de Jonge, Luuk van Iperen, Josette Gevers, Steven Vos
Background: Running-related injuries (RRIs) can be considered the primary enemy of runners. Most literature on injury prediction and prevention overlooks the mental aspects of overtraining and under-recovery, despite their potential role in injury prediction and prevention. Consequently, knowledge on the role of mental aspects in RRIs is lacking. Objective: To investigate mental aspects of overtraining and under-recovery by means of an online injury prevention programme...
2018: BMJ Open Sport & Exercise Medicine
Shveta Mahajan, Sudesh Kumar Mittal, Amitava Das
The conventional methods for seed quality testing have several limitations as they involve visual assessment and are destructive. In this context, a study was performed to assess the suitability of non-contact, non-destructive type imaging techniques such as visible imaging and X-ray imaging for conducting physical purity, viability and vigour tests of soybean seeds. The seeds that appeared healthy in external surface examination using visible tests as well as in internal assessment using X-ray tests were classified as sound seeds while the other seeds were marked as not-sound seeds...
October 2018: Journal of Food Science and Technology
Wang Ying, Wang Chunxia, Zhang Jukui, Wang Chunqing
Resource availability influences plant growth and reproduction. Here, a controlled experiment was conducted in order to evaluate the adaptation response of Chloris virgata to different precipitation conditions, and to further predict the reproductive strategy in a population of C. virgata under different precipitation regimes. Three regimes (low, typical and high) of water addition were used to simulate current precipitation patterns. In total 20 individuals for each treatment were analysed to compare tiller number, spike traits, seed traits, the relationship between seed size and seed number, and so on...
August 2018: Royal Society Open Science
José A B Bezerra, Andréia M Silva, Patrícia C Sousa, Lívia B Campos, Érica C G Praxedes, Luana G P Bezerra, Thibério S Castelo, Ana L P Souza, Alexandre R Silva
SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed...
August 2018: Zygote: the Biology of Gametes and Early Embryos
Merryn G Smith, Stefan K Arndt, Rebecca E Miller, Sabine Kasel, Lauren T Bennett
Non-structural carbohydrates (NSCs) are crucial to support tree resprouting after disturbances that damage the crown or stem. Epicormic resprouting (from stem) could demand more from NSC reserves than basal resprouting (following top-kill), since epicormically resprouting trees need to maintain a greater living biomass. Yet, little is known about NSC use during epicormic resprouting, particularly the relative importance of stem and below-ground NSC reserves. We compared the distribution and magnitude of NSC decreases during epicormic and basal resprouting by experimentally removing crowns or stems of 14-year-old Eucalyptus obliqua L'Hér...
September 14, 2018: Tree Physiology
Mohar Singh, Savita Rani, Nikhil Malhotra, Gopal Katna, Ashutosh Sarker
The wild species of chickpea have tremendous potential for enhancing genetic gains of cultigen and have resistant accessions against major biotic and abiotic stresses. In the present study, two wild annual accessions, one each of C. reticulatum Ladiz. (ILWC 229) and C. echinospermum Davis (ILWC 246) were assessed for their agro-morphological features and hybridized with different cultivated varieties (BGD 72, PBG 5, ICKG 96029, Pusa 372 and JG 11) of chickpea. Fertile F1 plants were developed as revealed by their normal meiotic chromosomal configuration including high pollen stainability percentage and seed set...
2018: PloS One
M Bossley, A Steiner, P Brakes, J Shrimpton, C Foster, L Rendell
Social learning of adaptive behaviour is widespread in animal populations, but the spread of arbitrary behaviours is less common. In this paper, we describe the rise and fall of a behaviour called tail walking, where a dolphin forces the majority of its body vertically out of the water and maintains the position by vigourously pumping its tail, in a community of Indo-Pacific bottlenose dolphins ( Tursiops aduncus ). The behaviour was introduced into the wild following the rehabilitation of a wild female individual, Billie, who was temporarily co-housed with trained dolphins in a dolphinarium...
September 2018: Biology Letters
Daniela Haluza, Verena-Maria Schmidt, Gerhard Blasche
AIM: To explore how nurses recover from night shift work during periods off duty. BACKGROUND: Given the large number of affected individuals globally, detrimental health outcomes of night shift work are an important public health issue. Sufficient recovery opportunities are essential to prevent prolonged fatigue associated with demanding tasks and high workload as experienced in nurses working long shifts during the night. METHOD: Nurses (n = 53, 96% females) who worked in two public nursing homes in Austria completed a 5-day diary to collect data on well-being conceptualized by fatigue, distress and vigour...
September 4, 2018: Journal of Nursing Management
Greg Deakin, Emma L Tilston, Julie Bennett, Tom Passey, Nicola Harrison, Felicidad Fernández-Fernández, Xiangming Xu
Characterising spatial microbial community structure is important to understand and explain the consequences of continuous plantation of one crop species on the performance of subsequent crops, especially where this leads to reduced growth vigour and crop yield. We investigated the spatial structure, specifically distance-decay of similarity, of soil bacterial and fungal communities in two long-established orchards with contrasting agronomic characteristics. A spatially explicit sampling strategy was used to collect soil from under recently grubbed rows of apple trees and under the grassed aisles...
September 2018: Applied Soil Ecology: a Section of Agriculture, Ecosystems & Environment
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