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https://www.readbyqxmd.com/read/27913217/integration-of-whole-cell-reaction-and-product-isolation-highly-hydrophobic-solvents-promote-in-situ-substrate-supply-and-simplify-extractive-product-isolation
#1
Dorothea Leis, Bernhard Lauß, Robert Macher-Ambrosch, Andreas Pfennig, Bernd Nidetzky, Regina Kratzer
Product isolation from aqueous-organic reaction mixtures that contain high concentrations of whole cells constitutes a challenging task in bioprocessing. Stirring of the biphasic reaction media leads to the formation of solvent droplets coated by surface active components and an emulsion forms. We used an early focus on phase separation to simplify a whole-cell bioreduction. Octanol, heptanol, hexanol, hexane and dipropylether were tested as co-solvents in the E. coli catalyzed reduction of o-chloroacetophenone...
November 29, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27912125/feooh-graphene-oxide-nanocomposites-for-fluoride-removal-from-water-acetate-mediated-nano-feooh-growth-and-adsorption-mechanism
#2
Liyuan Kuang, Yuyang Liu, Dandan Fu, Yaping Zhao
Fluoride adsorption capacity in water matrices depends highly on the properties of each component of the adsorption system, the most important one of these is the physchemical properties of the adsorbent. Nanoparticle Goethite anchored onto graphene oxide (FeOOH+Ac/GO) and rice spike-like Akaganeite anchored onto graphene oxide (FeOOH/GO) were synthesized via an in-situ hydrolysis procedure and compared their fluoride adsorption performances in order to address the effect of crystalline structure growth induced by acetate sodium (NaAc), one important organic ligand in water and soil...
November 21, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27897983/effects-of-polysaccharide-elicitors-from-endophytic-fusarium-oxysporum-fat9-on-the-growth-flavonoid-accumulation-and-antioxidant-property-of-fagopyrum-tataricum-sprout-cultures
#3
Lingyun Zhong, Bei Niu, Lin Tang, Fang Chen, Gang Zhao, Jianglin Zhao
The purpose of this study was to evaluate the effects of four different fungal polysaccharides, named water-extracted mycelia polysaccharide (WPS), sodium hydroxide-extracted mycelia polysaccharide (SPS), hydrochloric-extracted mycelia polysaccharide (APS), and exo-polysaccharide (EPS) obtained from the endophytic Fusarium oxysporum Fat9 on the sprout growth, flavonoid accumulation, and antioxidant capacity of tartary buckwheat. Without visible changes in the appearance of the sprouts, the exogenous polysaccharide elicitors strongly stimulated sprout growth and flavonoid production, and the stimulation effect was closely related with the polysaccharide (PS) species and its treatment dosage...
November 25, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27896446/biocompatible-silver-nanoparticles-prepared-with-amino-acids-and-a-green-method
#4
Ricardo Almeida de Matos, Lilia Coronato Courrol
The synthesis of nanoparticles is usually carried out by chemical reduction, which is effective but uses many toxic substances, making the process potentially harmful to the environment. Hence, as part of the search for environmentally friendly or green synthetic methods, this study aimed to produce silver nanoparticles (AgNPs) using only AgNO3, Milli-Q water, white light from a xenon lamp (Xe) and amino acids. Nanoparticles were synthetized using 21 amino acids, and the shapes and sizes of the resultant nanoparticles were evaluated...
November 28, 2016: Amino Acids
https://www.readbyqxmd.com/read/27875766/augmentation-of-bacterial-homeostasis-by-regulating-in-situ-buffer-capacity-significance-of-total-dissolved-salts-over-acidogenic-metabolism
#5
S Venkata Mohan, S Srikanth, G N Nikhil
During anaerobic fermentation, consequent accumulation of acidic fermented products leads to the failure of pH homeostasis. The present study aimed to comprehend the changes in buffering capacity with addition of sodium salts of hydroxide, bicarbonate and phosphate. The results showed notable augmentation in buffer capacity and cumulative hydrogen production (CHP) compared to control. The influential factor is the amount of undissociated volatile fatty acids released that affected the cell metabolism and consequently biohydrogen generation...
November 9, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27874897/extraction-of-nb-v-by-quaternary-ammonium-based-solvents-toward-organic-hexaniobate-systems
#6
Gauthier J-P Deblonde, Alexandre Chagnes, Marie-Aude Roux, Valérie Weigel, Gérard Cote
Solvent extraction of Nb(v) from alkaline aqueous media using quaternary ammonium solutions, especially Aliquat® 336 diluted in an aliphatic diluent, was investigated. The hexaniobate ions (HxNb6O19(x-8)) were extracted into the organic phase with very high yields at room temperature and within a few minutes, affording easy access to organic solutions of hexaniobates. Several parameters were found to influence the extraction of HxNb6O19(x-8) including the nature and concentration of alkali cations, confirming subtle effects previously described for polyoxoniobates such as ion-pairing with alkali ions...
November 22, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27859284/comparison-of-rehydration-techniques-for-fingerprinting-the-deceased-after-mummification
#7
Chun-Chieh Chen, Chao-Kai Yang, Chun-Yu Chen, Henry C Lee, Sheng-Meng Wang
Postmortem decay causes fingertip decomposition, desiccation, shriveling, and rigidity, reducing the possibility of obtaining sufficiently clear fingerprints for identification. In this study, five rehydration solutions (ammonium hydroxide, sodium carbonate, potassium hydroxide, urea, and warm water) followed by three fingerprint recording methods (photograph, inking roll, and dusting tape) were investigated to process mummified fingertips from an unidentified cadaver. The results show that sodium carbonate treatment is the most effective for minutiae restoration, followed by ammonium hydroxide treatment...
November 10, 2016: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27858036/fast-nucleation-for-silica-nanoparticle-synthesis-using-a-sol-gel-method
#8
Chandra K Dixit, Snehasis Bhakta, Ajeet Kumar, Steven L Suib, James F Rusling
We have developed a method that for the first time allowed us to synthesize silica particles in 20 minutes using a sol-gel preparation. Therefore, it is critically important to understand the synthesis mechanism and kinetic behavior in order to achieve a higher degree of fine tuning ability during the synthesis. In this study, we have employed our ability to modulate the physical nature of the reaction medium from sol-gel to emulsion, which has allowed us to halt the reaction at a particular time; this has allowed us to precisely understand the mechanism and chemistry of the silica polymerization...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27854308/characterization-of-chemically-induced-bacterial-ghosts-bgs-using-sodium-hydroxide-induced-vibrio-parahaemolyticus-ghosts-vpgs
#9
Hyun Jung Park, Sung Oh, Nagarajan Vinod, Seongmi Ji, Han Byul Noh, Jung Mo Koo, Su Hyeong Lee, Sei Chang Kim, Ki-Sung Lee, Chang Won Choi
Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the respective chemicals were 3.125, 6...
November 15, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27842836/fabrication-and-characterization-of-a-self-crosslinking-chitosan-hydrogel-under-mild-conditions-without-the-use-of-strong-bases
#10
Yongxiang Xu, Jianmin Han, Hong Lin
Self-crosslinking chitosan hydrogels are a highly suitable material for biomedical applications owing to their biodegradability and biocompatibility. However, strong bases, such as sodium hydroxide, which are often used in the preparation of such hydrogels, are known to affect biocompatibility and even destroy the bioactive factors or drug payload of the hydrogel. In the present study, strong bases were replaced by sodium chloride (NaCl) and phosphate buffer saline (PBS, pH=7.4), which were used as gelling solutions for hydrogel fabrication via the freeze-melting-neutralization method...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27833653/valorization-of-lignin-and-cellulose-in-acid-steam-exploded-corn-stover-by-a-moderate-alkaline-ethanol-post-treatment-based-on-an-integrated-biorefinery-concept
#11
Sheng Yang, Yue Zhang, Wen Yue, Wei Wang, Yun-Yan Wang, Tong-Qi Yuan, Run-Cang Sun
BACKGROUND: Due to the unsustainable consumption of fossil resources, great efforts have been made to convert lignocellulose into bioethanol and commodity organic compounds through biological methods. The conversion of cellulose is impeded by the compactness of plant cell wall matrix and crystalline structure of the native cellulose. Therefore, appropriate pretreatment and even post-treatment are indispensable to overcome this problem. Additionally, an adequate utilization of coproduct lignin will be important for improving the economic viability of modern biorefinery industries...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27827798/unimpaired-immunogenicity-of-yeast-expressed-hepatitis-b-surface-antigen-stored-at-elevated-temperatures
#12
Zhongliang Shen, Yuliang Rao, Shuai Tao, Mengjun Luo, Lijun Ming, Jing Liu, Shaokun Pan, Youhua Xie
Global adoption of hepatitis B virus (HBV) vaccines has greatly reduced new HBV infections. Current HBV vaccines are liquid suspensions containing recombinant hepatitis B surface antigen (HBsAg) particles mixed with aluminum phosphate or aluminum hydroxide. Refrigeration (2-8°C) as recommended for vaccine transport and storage may be unachievable in certain HBV-prevalent developing countries or regions. In this study, we stored yeast-expressed HBsAg and aluminum hydroxide separately at the standard (4°C) and elevated temperature (25, 37, or 45°C) for 14, 23, and 30 days, then mixed them and used the mixture to vaccinate mice with a prime-boost program...
November 8, 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27818094/comparative-study-of-mycobacterium-bovis-primary-isolation-methods
#13
Marina de Azevedo Issa, Paulo Martins Soares Filho, Antônio Augusto Fonseca Júnior, Mikael Arrais Hodon, Lílian Cristian Dos Santos, Jenner Karlisson Pimenta Dos Reis, Rômulo Cerqueira Leite
For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement "A" (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared...
September 30, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27817787/-effect-of-montelukast-sodium-intervention-on-airway-remodeling-and-percentage-of-th17-cells-cd4-cd25-regulatory-t-cells-in-asthmatic-mice
#14
Li Li, Chun-Yan Lou, Min Li, Jian-Bo Zhang, Jie Chen
OBJECTIVE: To study the dynamic changes in the percentage of Th17 cells/CD4(+)CD25(+) regulatory T cells after intervention with montelukast sodium, a leukotriene receptor antagonist, in asthmatic mice and the association between them. METHODS: Balb/c mice were randomly divided into blank group, asthma group, and montelukast sodium group. The asthmatic mouse model of airway remodeling was established by sensitization with intraperitoneal injection of chicken ovalbumin (OVA) and aluminum hydroxide suspension and aerosol inhalation of OVA...
November 2016: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/27805193/structure-of-aqueous-naoh-solutions-insights-from-neural-network-based-molecular-dynamics-simulations
#15
Matti Hellström, Jörg Behler
Sodium hydroxide, NaOH, is one of the most widely-used chemical reagents, but the structural properties of its aqueous solutions have only sparingly been characterized. Here, we automatically classify the cation coordination polyhedra obtained from molecular dynamics simulations. We find that, for example, with increasing concentration, octahedral coordination geometries become less favored, while the opposite is true for the trigonal prism. At high concentrations, the coordination polyhedra frequently deviate considerably from "ideal" polyhedra, because of an increased extent of interligand hydrogen-bonding, in which hydrogen bonds between two ligands, either OH2 or OH(-), around the same Na(+) are formed...
November 2, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27790252/antibacterial-efficacy-of-calcium-hydroxide-and-chlorhexidine-mixture-for-treatment-of-teeth-with-primary-endodontic-lesions-a-randomized-clinical-trial
#16
Zakiyeh Donyavi, Parastoo Ghahari, Mohammad Esmaeilzadeh, Mohammadjavad Kharazifard, Rasoul Yousefi-Mashouf
INTRODUCTION: This study compared the root canal microbial count of necrotic teeth after irrigation with 6% sodium hypochlorite (NaOCl) (single session treatment) and two-session root canal treatment with two-week application of calcium hydroxide (CH) mixed with 0.2% chlorhexidine (CHX) as intracanal medicament. METHODS AND MATERIALS: In this randomized clinical trial, single-rooted necrotic teeth were divided into two groups. Root canal was irrigated with 2 mL of 6% NaOCl in one group, and a mixture of 0...
2016: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/27784948/screening-of-bacillus-strains-from-sun-vinegar-for-efficient-production-of-flavonoid-and-phenol
#17
Changqing Zhao, Xingxiu Zhao, Jing Zhang, Wei Zou, Yi Zhang, Li Li, Jun Liu
The aim of this study was to screen out a bacillus from Sichuan sun vinegar grains capable of producing a high total amount of flavonoids and total phenols. Forty-eight strains were initially screened out from Sichuan sun vinegar grains. The sodium nitrite-aluminum nitrate-sodium hydroxide colorimetry method was used to determine the total flavonoids, and the Folin-phenol method was used to determine the total phenols. Based on the results of total flavonids and total phenol producing capacities of the strains, one strain was singled out for use in this study...
December 2016: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/27776867/amorphous-boron-doped-sodium-titanates-hydrates-efficient-and-reusable-adsorbents-for-the-removal-of-pb-2-from-water
#18
Luigi di Bitonto, Angela Volpe, Michele Pagano, Giuseppe Bagnuolo, Giuseppe Mascolo, Valeria La Parola, Paola Di Leo, Carlo Pastore
Amorphous titanium hydroxide and boron-doped (B-doped) sodium titanates hydrates were synthetized and used as adsorbents for the removal of Pb(2+) from water. The use of sodium borohydride (NaBH4) and titanium(IV) isopropoxide (TTIP) as precursors permits a very easy synthesis of B-doped adsorbents at 298K. The new adsorbent materials were first chemically characterized (XRD, XPS, SEM, DRIFT and elemental analysis) and then tested in Pb(2+) adsorption batch experiments, in order to define kinetics and equilibrium studies...
October 20, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27774559/1-1-diacetyloctamethylferrocene-an-overlooked-and-overdue-synthon-leading-to-the-facile-synthesis-of-an-octamethylferrocenophane
#19
Max Roemer, Brian W Skelton, Matthew J Piggott, George A Koutsantonis
This paper describes the facile preparation of 1,1'-diacetyloctamethylferrocene (2) by acylation of octamethylferrocene (1) with acetyl chloride. Chloroformylation with POCl3/DMF of 2 affords a variety of products, including 1,1'-bis-(1-chlorovinyl)octamethylferrocene (3b) in high yield. Compound 3b cyclises in aqueous sodium hydroxide/DMF to an octamethyl[1,4]-ferrocenophane bearing a 1-dimethylaminobuta-1,3-diene-handle (4).
October 24, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27773332/pad-ultrasonic-batch-dyeing-of-causticized-lyocell-fabric-with-reactive-dyes
#20
Aijaz Ahmed Babar, Mazhar Hussain Peerzada, Abdul Khalique Jhatial, Noor-Ul-Ain Bughio
Conventionally, cellulosic fabric dyed with reactive dyes requires significant amount of salt. However, the dyeing of a solvent spun regenerated cellulosic fiber is a critical process. This paper presents the dyeing results of lyocell fabrics dyed with conventional pad batch (CPB) and pad ultrasonic batch (PUB) processes. The dyeing of lyocell fabrics was carried out with two commercial dyes namely Drimarine Blue CL-BR and Ramazol Blue RGB. Dyeing parameters including concentration of sodium hydroxide, sodium carbonate and dwell time were compared for the two processes...
January 2017: Ultrasonics Sonochemistry
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