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https://www.readbyqxmd.com/read/29228714/unique-expression-signatures-of-circular-rnas-in-response-to-dna-tumor-virus-sv40-infection
#1
Jiandong Shi, Ningzhu Hu, Jianfang Li, Zhaoping Zeng, Ling Mo, Jing Sun, Meini Wu, Yunzhang Hu
Circular RNAs (circRNAs), identified as a class of widely expressed endogenous regulatory RNAs, are involved in diverse physiological and pathological processes. However, their role in viral pathogenesis and cellular antiviral response remains unexplored. In this study, a potent DNA tumor virus, simian virus 40 (SV40), was used as a model to investigate the viral influences on cellular circRNA transcriptome. Using RNA-seq, 15,241 putative circRNAs were identified de novo from 5,057 parental genes in monkey kidney-derived Vero cells...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29209895/antiviral-activity-and-metal-ion-binding-properties-of-some-2-hydroxy-3-methoxyphenyl-acylhydrazones
#2
M Carcelli, E Fisicaro, C Compari, L Contardi, D Rogolino, C Solinas, A Stevaert, L Naesens
Here we report on the results obtained from an antiviral screening, including herpes simplex virus, vaccinia virus, vesicular stomatitis virus, Coxsackie B4 virus or respiratory syncytial virus, parainfluenza-3 virus, reovirus-1 and Punta Toro virus, of three 2-hydroxy-3-methoxyphenyl acylhydrazone compounds in three cell lines (i.e. human embryonic lung fibroblast cells, human cervix carcinoma cells, and African Green monkey kidney cells). Interesting antiviral EC50 values are obtained against herpes simplex virus-1 and vaccinia virus...
December 5, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29190645/cd105-mesenchymal-stem-cells-migrate-into-osteoarthritis-joint-an-animal-model
#3
Pablo Fernandez-Pernas, Iván Rodríguez-Lesende, Alexandre de la Fuente, Jesús Mateos, Isaac Fuentes, Javier De Toro, Fco J Blanco, M C Arufe
Mesenchymal stem cells are being the focus of connective tissue technology and regenerative medicine, presenting a good choice cell source for improving old and well recognized techniques of cartilage defect repair. For instance, the autologous chondrocyte transplantation using new concepts of regenerative medicine. The present study investigated the risk of xenogenicity of human synovial membrane-derived MSCs, injected into the monkeys using intravenous and intra-articular administration. The animal models used were adult monkeys Rhesus which had been injured into the left knee to create an Osteoarthritis (OA) animal model...
2017: PloS One
https://www.readbyqxmd.com/read/29173547/bobcats-lynx-rufus-are-natural-definitive-host-of-besnoitia-darlingi
#4
Shiv K Verma, Camila K Cerqueira-Cézar, Fernando H A Murata, Matthew J Lovallo, Benjamin M Rosenthal, Jitender P Dubey
Bovine besnoitiosis, caused by Besnoitia besnoiti, is an economically important disease of cattle in many countries but its transmission remains a mystery. Wild felids are suspected to be its definitive hosts. The domestic cat (Felis catus) is known experimental definitive host for Besnoitia species of rodents. Here, we report for Besnoitia darlingi the first identification of a natural definitive host, the bobcat (Lynx rufus). Oocysts resembling Toxoplasma gondii (unsporulated; 10.9±0.8×12.1±0.2μm; n=5) were detected microscopically in the feces of two of 25 free ranging wild bobcats from Mississippi, USA...
December 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29150986/memory-t-cells-are-significantly-increased-in-rejecting-liver-allografts-of-rhesus-monkeys
#5
Hwajung Kim, Hyeyoung Kim, Sun-Kyung Lee, Xue-Li Jin, Tae Jin Kim, Chanho Park, Jae-Il Lee, Hyo-Sin Kim, Suk Kyun Hong, Kyung Chul Yoon, Sung Woo Ahn, Kyoung-Bun Lee, Nam-Joon Yi, Jaeseok Yang, Kwang-Woong Lee, Wayne J Hawthorne, Kyung-Suk Suh
INTRODUCTION: The Rhesus monkey (RM) is an excellent preclinical model in kidney, heart and islet transplantation that has provided the basis for new immunosuppressive protocols for clinical studies. However, there remain relatively few liver transplantation (LT) models in nonhuman primates. In this study, we analyzed the immune cell populations of PBMCs and secondary lymphoid organs along with livers of normal rhesus monkeys and compared them to those of rejecting liver transplanted recipient's following withdrawal of immunosuppression...
November 18, 2017: Liver Transplantation
https://www.readbyqxmd.com/read/29150543/toxicokinetics-and-physiologically-based-pharmacokinetic-modeling-of-the-shellfish-toxin-domoic-acid-in-nonhuman-primates
#6
Jing Jing, Rebekah Petroff, Sara Shum, Brenda Crouthamel, Ariel R Topletz, Kimberly S Grant, Thomas M Burbacher, Nina Isoherranen
Domoic acid (DA), a neurotoxin, is produced by marine algae and has caused toxications worldwide in animals and humans. However, the toxicokinetics of DA has not been fully evaluated, and information is missing on the disposition of DA following oral exposures at doses that are considered safe for human consumption. In this study, toxicokinetics of DA were investigated in cynomolgus monkeys, following single doses of 5 µg/kg DA iv, 0.075 mg/kg DA po and 0.15 mg/kg DA po. Following iv dosing, DA had a systemic clearance of 124 ± 71 ml/hr/kg, volume of distribution at steady state of 131±71 ml/kg and elimination half-life of 1...
November 17, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29149329/polymyxin-derivatives-nab739-and-nab815-are-more-effective-than-polymyxin-b-in-murine-escherichia-coli-pyelonephritis
#7
Martti Vaara, Timo Vaara, Carina Vingsbo Lundberg
Objectives: Extremely multiresistant strains of Enterobacteriaceae, such as those of Escherichia coli and Klebsiella pneumoniae, are emerging and spreading at a worrisome speed. Polymyxins (polymyxin B, colistin) are used as last-line therapy against such strains, in spite of their notable nephrotoxicity that may even require discontinuation of the therapy. We have previously developed polymyxin derivatives NAB739 and NAB815 that are better tolerated in cynomolgus monkeys than polymyxin B and are, in contrast to polymyxin B, excreted in the cynomolgus urine to a very significant degree...
November 14, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29126431/successful-long-term-maintenance-of-mansonella-perstans-in-an-in-vitro-culture-system
#8
Abdel Jelil Njouendou, Manuel Ritter, Winston Patrick Chounna Ndongmo, Chi Anizette Kien, Gandjui Tchamatchoua Victor Narcisse, Fanny Fri Fombad, Dizzle Bita Tayong, Kenneth Pfarr, Laura E Layland, Achim Hoerauf, Samuel Wanji
BACKGROUND: Approximately 114 million people are infected with Mansonella perstans in large proportions of Africa. In contrast to other filariae that infect humans, M. perstans-infected individuals show no distinct pathology or specific clinical picture, indicating a well-tuned adaptation to the host. In addition, since M. perstans adult worms reside in serous cavities which are difficult to access, research has been hindered and there is a paucity of knowledge about the biology of M...
November 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29103685/cholesterol-25-hydroxylase-is-an-interferon-inducible-factor-that-protects-against-porcine-reproductive-and-respiratory-syndrome-virus-infection
#9
Zhongbao Song, Qiaoya Zhang, Xuewei Liu, Juan Bai, Yongxiang Zhao, Xianwei Wang, Ping Jiang
Porcine reproductive and respiratory syndrome virus (PRRSV), a single-stranded, positive-sense RNA virus of the Arteriviridae family, has become a global health threat for swine. Cholesterol 25-hydroxylase (CH25H) is an enzyme that catalyzes oxidation of cholesterol to 25-hydroxycholesterol (25HC). The purpose of this study was to explore the antiviral activity of CH25H against PRRSV infection. We found that CH25H was induced by interferon-α and PRRSV in Marc-145 monkey kidney cells. In addition, CH25H and 25HC significantly inhibited PRRSV infection by preventing virus entry...
October 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29067207/abdominal-ultrasonography-of-the-healthy-howler-monkey-alouatta-fusca
#10
Raquel Sartor, Thiago R Müller, Maria J Mamprim, Carlos R Teixeira, Livia P de Souza, Ricardo C Lehmkuhl, Marilia G Luciani
Abdominal ultrasonography was performed in six healthy adult brown howler monkeys Alouatta fusca and the normal ultrasonographic anatomy of the liver, gallbladder, stomach and urinary tract described for the first time. Findings were compared with post-mortem studies. The renal cortex was isoechoic to the spleen and isoechoic or hyperechoic to the liver. Kidney length and renal arterial resistive index, systolic and diastolic velocity were calculated. The liver showed a homogeneous hypoechogenic echotexture...
February 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29059459/molecular-cloning-and-tissue-distribution-of-a-novel-marmoset-abc-transporter
#11
Shotaro Uehara, Yasuhiro Uno, Takashi Inoue, Erika Sasaki, Hiroshi Yamazaki
Common marmosets (Callithrix jacchus) have been recognized as a useful small non-human primate model in preclinical testing for drug development. In this study, a cDNA of novel ATP-dependent efflux transporter ABCB1 was cloned from marmoset liver tissue. Marmoset ABCB1 cDNA encodes a protein of 1,279 amino acid residues (MW = 141.4 kDa) containing characteristic regions of an ATP-binding cassette (ABC) protein, two hydrophobic transmembrane regions, and two cytoplasmic nucleotide-binding regions, similar to human ABCB1...
October 23, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/29051342/-68-ga-nota-functionalized-ubiquicidin-cytotoxicity-biodistribution-radiation-dosimetry-and-first-in-human-positron-emission-tomography-computed-tomography-imaging-of-infections
#12
Thomas Ebenhan, Mike Sathekge, Thabo Lenagana, Michel Koole, Olivier Gheysens, Thavendran Govender, Jan R Zeevaart
Ubiquicidin is an antimicrobial peptide (AMP) with great potential for nuclear imaging of infectious diseases as its cationic-rich fragment TGRAKRRMQYNRR (UBI) has been functionalized with 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) to allow complexation to Gallium-68 ((68)Ga-NOTA-UBI). We herein assess the cytotoxicity, radiation dosimetry for (68)Ga-NOTA-UBI and a first-in-human evaluation to diagnose infectious processes. Methods: Cytotoxicity was evaluated in green monkey kidney epithelial (Vero) cells and MT-4 leucocytes...
October 19, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29051111/perspectives-on-using-a-multiplex-human-kidney-safety-biomarker-panel-to-detect-cisplatin-induced-tubular-toxicity-in-male-and-female-cynomolgus-monkeys
#13
Yafei Chen, J Dale Thurman, Lewis B Kinter, Russell Bialecki, J Eric McDuffie
Multiplex biomarker panel assays would enable early de-risking of discovery compound related kidney safety liabilities. The objective of this study was to evaluate the usefulness of the Myriad RBM Human KidneyMAP (Multi-Analyte Profile)® v.1.0 panel to detect experimental nephrotoxicity in Cynomolgus monkeys following a single intravenous administration of cisplatin (2.5mg/kg). Urine samples were collected at baseline on day -2; at approximately 4hr post-dose on day 1; and on days 4, 9, 15 and/or 20. Blood samples were collected at predose on day -2; at 4hr post-dose on day 1; and on days 2, 5, 10 and/or 21...
October 16, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29025984/synthesis-and-biological-evaluation-of-a-novel-18-f-labeled-adnectin-as-a-pet-radioligand-for-imaging-pd-l1-expression
#14
David J Donnelly, Ralph Adam Smith, Paul Morin, Dasa Lipovsek, Jochem Gokemeijer, Daniel Cohen, Virginie Lafont, Tritin Tran, Erin Lee Cole, Martin Wright, Joonyoung Kim, Adrienne Pena, Daniel Kukral, Douglas D Dischino, Patrick Chow, Jinping Gan, Olufemi Adelakun, Xi-Tao Wang, Kai Cao, David Lueng, Samuel Bonacorsi, Wendy Hayes
The programmed death protein (PD-1) and its ligand (PD-L1) play critical roles in a checkpoint pathway cancer cells exploit to evade the immune system. A same-day PET imaging agent for measuring PD-L1 status in primary and metastatic lesions could be important for optimizing drug therapy. Herein, we have evaluated the tumor targeting of an anti-PD-L1 Adnectin after (18)F-fluorine labeling. Methods: An anti-PD-L1 Adnectin was labeled with (18)F in two steps. This synthesis featured fluorination of a novel prosthetic group, followed by a copper-free click conjugation to a modified Adnectin to generate (18)F-BMS-986192...
October 12, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29017779/targeted-inhibition-of-hantavirus-replication-and-intracranial-pathogenesis-by-a-chimeric-protein-delivered-sirna
#15
Jie Yang, Ji-Feng Sun, Ting-Ting Wang, Xiao-Hong Guo, Jun-Xia Wei, Lin-Tao Jia, An-Gang Yang
Hantavirus (HV) infection, which underlies hantavirus hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, remains to be a severe clinical challenge. Here, we synthesized small interfering RNAs (siRNAs) that target the encoding sequences of HV strain 76-118, and validated their inhibitory role in virus replication in HV-infected monkey kidney Vero E6 cells. A chimeric protein, 3G1-Cκ-tP, consisting of a single-chain antibody fragment (3G1) against the HV surface envelop glycoprotein, the constant region of human immunoglobulin κ chain (Cκ), and truncated protamine (amino acids 8-29, tP), was further generated...
October 7, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28959669/in-vitro-cytotoxicity-of-zinc-oxide-iron-oxide-and-copper-nanopowders-prepared-by-green-synthesis
#16
Saranya S, Vijayaranai K, Pavithra S, Raihana N, Kumanan K
In vitro cytotoxic effects of ZnO, FeO and Cu metallic nanopowders (NPs) on Vero (African green monkey kidney cell line), PK 15 (Pig kidney cell line) and Madin Darby Bovine Kidney (MDBK) cell lines were investigated at different time intervals (24 and 48 h). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to determine the cytotoxic effects of green synthesized (plant based) nanopowders. The comparative effects of exposure period and concentration of nanopowders on cell viability were studied...
2017: Toxicology Reports
https://www.readbyqxmd.com/read/28955630/synthesis-characterization-and-biological-applications-of-mycosynthesized-silver-nanoparticles
#17
Sathiyaseelan Anbazhagan, Shajahan Azeez, Girilal Morukattu, Ramachandran Rajan, Kaviyarasan Venkatesan, Kalaichelvan Puthupalayam Thangavelu
Silver nanoparticles (AgNPs) have been known for their inhibitory and bactericidal effects. In the present study, less toxic AgNPs using Cunninghamella echinulata is reported for the first time. The obtained AgNPs were characterized using UV-Visible spectrophotometer, XRD, FT-IR, FE-SEM with EDAX and HR-TEM. AgNPs showed the maximum absorbance at 420-430 nm. The transmission electron micrograph revealed the formation of considerably uniform-sized AgNPs with an average size of 20-50 nm. The reducing and capping agents responsible for AgNP synthesis were identified by FT-IR...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28942525/subcutaneous-ovarian-tissue-transplantation-in-nonhuman-primates-duration-of-endocrine-function-and-normalcy-of-subsequent-offspring-as-demonstrated-by-reproductive-competence-oocyte-production-and-telomere-length
#18
David M Lee, Carrie M Thomas, Fuhua Xu, Richard R Yeoman, Jing Xu, Richard L Stouffer, Don P Wolf, Mary B Zelinski
PURPOSE: The main purposes of the study were to investigate the endocrine function of ovarian tissue transplanted to heterotopic subcutaneous sites and the reproductive competence and telomere length of a nonhuman primate originating from transplanted tissue. METHODS: Ovarian cortex pieces were transplanted into the original rhesus macaques in the arm subcutaneously, in the abdomen next to muscles, or in the kidney. Serum estradiol (E2) and progesterone (P4) concentrations were measured weekly for up to 8 years following tissue transplantation...
September 23, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28918011/nucleic-acid-polymers-with-accelerated-plasma-and-tissue-clearance-for-chronic-hepatitis-b-therapy
#19
Ingo Roehl, Stephan Seiffert, Celia Brikh, Jonathan Quinet, Catherine Jamard, Nadine Dorfler, Jennifer A Lockridge, Lucyna Cova, Andrew Vaillant
REP 2139 is a nucleic acid polymer (NAP) currently under clinical development for chronic hepatitis B (HBV) therapy. This preclinical study investigated different REP 2139 analogs that would display reduced accumulation in the serum and tissues, while retaining an antiviral effect against HBV infection. REP 2139 analogs were evaluated in human plasma, CD-1 mice, cynomolgus monkeys, and Pekin ducks. Discrete ribose transformation to 2'OH in selected riboadenosines resulted in a slow degradation in acidified human plasma that plateaued after 48 hr...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28904298/usefulness-of-urinary-biomarkers-for-nephrotoxicity-in-cynomolgus-monkeys-treated-with-gentamicin-cisplatin-and-puromycin-aminonucleoside
#20
Hiroshi Uchino, Junko Fujishima, Kaori Fukuoka, Teppei Iwakiri, Akira Kamikuri, Hidenori Maeda, Kazuhiro Nakama
The objective of this study was to investigate the availability of novel urinary biomarkers (BMs) such as total protein, albumin, β2-microglobulin, clusterin, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL) for the detection of acute nephrotoxicity in cynomolgus monkeys. Animals (total 9 males/3 groups) were administered gentamicin (GM) subcutaneously at 40 mg/kg for 7 days, cisplatin (CDDP) intravenously at 3 mg/kg once and puromycin aminonucleoside (PAN) intravenously at 20 mg/kg for 7 days...
2017: Journal of Toxicological Sciences
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