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https://www.readbyqxmd.com/read/28525300/regulation-of-cell-polarity-in-motility-and-cell-division-in-myxococcus-xanthus
#1
Dominik Schumacher, Lotte Søgaard-Andersen
Rod-shaped Myxococcus xanthus cells are polarized with proteins asymmetrically localizing to specific positions. This spatial organization is important for regulation of motility and cell division and changes over time. Dedicated protein modules regulate motility independent of the cell cycle, and cell division dependent on the cell cycle. For motility, a leading-lagging cell polarity is established that is inverted during cellular reversals. Establishment and inversion of this polarity are regulated hierarchically by interfacing protein modules that sort polarized motility proteins to the correct cell poles or cause their relocation between cell poles during reversals akin to a spatial toggle switch...
May 19, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28516401/endovascular-crossing-of-chronic-total-occlusions-using-an-impulse-an-explorative-design-study
#2
Aimée Sakes, Marleen van der Wiel, Dimitra Dodou, Paul Breedveld
In this study we investigated whether exerting an impulse on a Chronic Total Occlusion (CTO) improves the success rate of CTO crossing as compared to the currently used method of statically pushing the guidewire against the CTO. A prototype (Ø2 mm) was developed that generates translational momentum using a spring-loaded indenter and converts it to an impulse during impact. Mechanical performance was evaluated by measuring the peak force and momentum for different spring compressions and strike distances in air and blood-mimicking fluid...
May 17, 2017: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/28512374/description-of-enchodorus-yeatsi-n-sp-dorylaimida-nordiidae-from-southern-iran-and-its-molecular-phylogenetic-study
#3
Majid Pedram
Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-µm long slender body, angular lip region having prominent papillae, 12- to 13-µm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic-amphidelphic reproductive system, and 102- to 160-µm long elongate-conoid tail with rounded tip...
March 2017: Journal of Nematology
https://www.readbyqxmd.com/read/28500523/cell-cycle-machinery-in-bacillus-subtilis
#4
Jeff Errington, Ling Juan Wu
Bacillus subtilis is the best described member of the Gram positive bacteria. It is a typical rod shaped bacterium and grows by elongation in its long axis, before dividing at mid cell to generate two similar daughter cells. B. subtilis is a particularly interesting model for cell cycle studies because it also carries out a modified, asymmetrical division during endospore formation, which can be simply induced by starvation. Cell growth occurs strictly by elongation of the rod, which maintains a constant diameter at all growth rates...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28487362/complex-binding-pathways-determine-the-regeneration-of-mammalian-green-cone-opsin-with-a-locked-retinal-analogue
#5
Nathan S Alexander, Kota Katayama, Wenyu Sun, David Salom, Sahil Gulati, Jianye Zhang, Muneto Mogi, Krzysztof Palczewski, Beata Jastrzebska
Phototransduction is initiated when the absorption of light converts the 11-cis-retinal chromophore to its all-trans configuration in both rod and cone vertebrate photoreceptors. To sustain vision, 11-cis-retinal is continuously regenerated from its all-trans conformation through a series of enzymatic steps comprising the visual or retinoid cycle. Abnormalities in this cycle can compromise vision because of the diminished supply of 11-cis-retinal and the accumulation of toxic, constitutively active opsin. As shown previously for rod cells, attenuation of constitutively active opsin can be achieved with the unbleachable analogue, 11-cis-6-membered ring (11-cis-6mr)-retinal, which has therapeutic effects against certain degenerative retinal diseases...
May 9, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28486184/bioaccessibility-of-polycyclic-aromatic-hydrocarbons-in-activated-carbon-or-biochar-amended-vegetated-salix-viminalis-soil
#6
Patryk Oleszczuk, Paulina Godlewska, Danny D Reible, Piotr Kraska
The aim of the present study was to determine the effect of activated carbon (AC) or biochars on the bioaccessibility (Cbioacc) of polycyclic aromatic hydrocarbons (PAHs) in soils vegetated with willow (Salix viminalis). The study determined the effect of willow on the Cbioacc PAHs and the effect of the investigated amendments on changes in dissolved organic carbon (DOC), crop yield and the content of PAHs in plants. PAH-contaminated soil was amended with 2.5 wt% AC or biochar. Samples from individual plots with and without plants were collected at the beginning of the experiment and after 3, 6, 12 and 18 months...
May 6, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28459777/analysis-of-explanted-magnetically-controlled-growing-rods-from-7-uk-spinal-centers
#7
Thomas J Joyce, Simon L Smith, Paul R P Rushton, Andrew J Bowey, Michael J Gibson
STUDY DESIGN: Analysis of explanted MAGnetic Expansion Control (MAGEC) growing rods. OBJECTIVE: To analyze explanted MAGEC rods used in management of early onset scoliosis and identify the mode of failure in such cases. SUMMARY OF BACKGROUND DATA: Magnetically controlled growing rods are increasingly used as the option of choice for early onset scoliosis. However, being more complex than conventional growing rods they are perhaps more likely to succumb to multifarious failure modes...
April 28, 2017: Spine
https://www.readbyqxmd.com/read/28453390/the-torments-of-the-cohesin-ring
#8
Alap P Chavda, Keven Ang, Dmitri Ivanov
Cohesin is a ring-shaped protein complex which comprises the Smc1, Smc3 and Scc1 subunits. It topologically embraces chromosomal DNA to connect sister chromatids and stabilize chromatin loops. It is required for proper chromosomal segregation, DNA repair and transcriptional regulation. We have recently reported that cohesin rings can adopt a "collapsed" rod-like conformation which is driven by the interaction between the Smc1 and Smc3 coiled coil arms and is regulated by post-translational modifications. The "collapsed" conformation plays a role in cohesin ring assembly and its loading on the DNA...
February 27, 2017: Nucleus
https://www.readbyqxmd.com/read/28449859/the-role-of-the-intramedullary-implant-in-limb-lengthening
#9
Peter R Calder, Maritz Laubscher, W David Goodier
Limb lengthening is now an accepted practice in orthopaedic surgery. The principles of distraction osteogenesis have become well established with the use of external fixators, utilizing both monolateral and ring fixators. Corticotomy technique, frame stability, lengthening rate and rhythm all contribute to the formation of bone regenerate and tissues. Complications are however common including pin-site infection, soft tissue tethering from the pins and wires resulting in pain, regenerate deformity from soft tissue forces or fracture following frame removal and patient intolerance of the frames during treatment...
April 24, 2017: Injury
https://www.readbyqxmd.com/read/28413834/synthesis-and-characterization-of-zinc-oxide-thin-films-for-optoelectronic-applications
#10
E Muchuweni, T S Sathiaraj, H Nyakotyo
Micro-ring structured zinc oxide (ZnO) thin films were prepared on glass substrates by spray pyrolysis and their structural, morphological, optical and electrical properties were investigated. X-ray Diffraction (XRD) analysis revealed the films' hexagonal wurtzite phase with a preferred (002) grain orientation. The mean crystallite size calculated on the basis of the Debye-Scherrer model was 24 nm and a small dislocation density of [Formula: see text] was obtained, indicating the existence of few lattice defects and good crystallinity...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28413261/sculpting-silica-colloids-by-etching-particles-with-nonuniform-compositions
#11
Fabian Hagemans, Wessel Vlug, Chiara Raffaelli, Alfons van Blaaderen, Arnout Imhof
We present the synthesis of new shapes of colloidal silica particles by manipulating their chemical composition and subsequent etching. Segments of silica rods, prepared by the ammonia catalyzed hydrolysis and condensation of tetraethylorthosilicate (TEOS) from polyvinylpyrrolidone loaded water droplets, were grown under different conditions. Upon decreasing temperature, delaying ethanol addition, or increasing monomer concentration, the rate of dissolution of the silica segment subsequently formed decreased...
April 11, 2017: Chemistry of Materials: a Publication of the American Chemical Society
https://www.readbyqxmd.com/read/28412013/investigation-of-the-imaging-characteristics-of-the-albira-ii-small-animal-pet-system-for-18-f-68-ga-and-64-cu
#12
Ali Asgar Attarwala, Yvonne Wanjiku Karanja, Deni Hardiansyah, Chiara Romanó, Mareike Roscher, Björn Wängler, Gerhard Glatting
AIM: In this study the performance characteristics of the Albira II PET sub-system and the response of the system for the following radionuclides (18)F, (68)Ga and (64)Cu was analyzed. MATERIALS AND METHODS: The Albira II tri-modal system (Bruker BioSpin MRI GmbH, Ettlingen, Germany) is a pre-clinical device for PET, SPECT and CT. The PET sub-system uses single continuous crystal detectors of lutetium yttrium orthosilicate (LYSO). The detector assembly consists of three rings of 8 detector modules...
April 12, 2017: Zeitschrift Für Medizinische Physik
https://www.readbyqxmd.com/read/28389960/structure-of-the-sodium-driven-flagellar-motor-in-marine-vibrio
#13
Yasuhiro Onoue, Michio Homma
Most bacteria can swim by rotating the flagellum. The basal body of the flagellum is an essential part for this motor function. Recent comprehensive analysis of the flagellar basal body structures across bacteria by cryo-electron tomography has revealed that they all share core structures, the rod, and rings: the C ring, M ring, S ring, L ring, and P ring. Furthermore, it also has uncovered that in some bacteria, there are extra ring structures in the periplasmic space and outer-membrane. Here, we describe a protocol to isolate the basal body of the flagellar basal body from a marine bacterium, Vibrio alginolyticus, for structural analysis of additional ring structures, the T ring and H ring...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28370231/radius-and-ulna-fracture-repair-with-the-imex-miniature-circular-external-skeletal-fixation-system-in-37-small-and-toy-breed-dogs-a-retrospective-study
#14
Denise Bierens, Marcos D Unis, Sady Y Cabrera, Philip H Kass, Tina J Owen, Maureen G Mueller
OBJECTIVE: To determine outcome and prognostic factors after radius and ulna fracture repair with a modified IMEX miniature circular external skeletal fixation (MCESF) in small and toy breed dogs. STUDY DESIGN: Retrospective study. SAMPLE POPULATION: Thirty-seven young small and toy breed dogs with 41 radial and ulnar fractures. METHODS: Records of radial and ulnar fractures repaired with a modified IMEX MCESF were reviewed...
March 28, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/28357498/computer-aided-analysis-for-optimal-screw-insertion-in-lateral-mass-of-c1-an-anatomical-study
#15
Renate Krassnig, Jakob Andrea Orlandi, Ellen Tackner, Gloria Hohenberger, Paul Puchwein
INTRODUCTION: Motion preserving techniques in C1 ring fractures are increasingly used especially in young patients. Therefore, lateral mass screws are inserted in the first vertebra and connected by a rod. The purpose of this study was to determine safe zones regarding the vertebral arteries and the medulla oblongata for optimal lateral mass screw positioning when fusing the C1-ring. MATERIALS AND METHODS: Images of the cervical spine of 50 patients (64-line CT scanner) were evaluated and virtual screws were positioned in both lateral masses of the first vertebra using 3D-reconstructions of CT scans...
March 29, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28327156/mycobacterium-goodii-endocarditis-following-mitral-valve-ring-annuloplasty
#16
Rohan B Parikh, Matthew Grant
BACKGROUND: Mycobacterium goodii is an infrequent human pathogen which has been implicated in prosthesis related infections and penetrating injuries. It is often initially misidentified as a gram-positive rod by clinical microbiologic laboratories and should be considered in the differential diagnosis. CASE PRESENTATION: We describe here the second reported case of M. goodii endocarditis. Species level identification was performed by 16S rDNA (ribosomal deoxyribonucleic acid) gene sequencing...
March 21, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28272311/synthesis-and-biological-activities-of-ethyl-2-2-pyridylacetate-derivatives-containing-thiourea-1-2-4-triazole-thiadiazole-and-oxadiazole-moieties
#17
Daniel Szulczyk, Piotr Tomaszewski, Michał Jóźwiak, Anna E Kozioł, Tadeusz Lis, David Collu, Filippo Iuliano, Marta Struga
Thirty six novel heterocyclic derivatives of ethyl 2-(2-pyridylacetate) were efficiently synthesized. The new compounds involve the linkage of a 2-pyridyl ring with thiosemicarbazide (compounds 1-7), 1,2,4-triazole (compounds 1a-7a), 1,3,4-thiadiazole (compounds 1b-7b), and 1,3,4-oxadiazole (compounds 1f-7f) moieties. The last group of compounds 1e-7e involves the connection of a 2-pyridyl ring with 1,2,4-triazole and thiourea. ¹H-NMR, (13)C-NMR and MS methods were used to confirm the structures of the obtained derivatives...
March 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28270223/biomechanical-characteristics-of-fixation-methods-for-floating-pubic-symphysis
#18
COMPARATIVE STUDY
Wenhao Song, Dongsheng Zhou, Yu He
BACKGROUND: Floating pubic symphysis (FPS) is a relatively rare injury caused by high-energy mechanisms. There are several fixation methods used to treat FPS, including external fixation, subcutaneous fixation, internal fixation, and percutaneous cannulated screw fixation. To choose the appropriate fixation, it is necessary to study the biomechanical performance of these different methods. The goal of this study was to compare the biomechanical characteristics of six methods by finite element analysis...
March 7, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28236073/complications-after-percutaneous-internal-fixator-for-anterior-pelvic-ring-injuries
#19
Christian Fang, Hatem Alabdulrahman, Hans-Christophe Pape
OBJECTIVES: The aim of the study is to report on the observed incidence of complications following pedicle screw and rod internal fixator (INFIX) stabilization of pelvic ring fractures. METHODS: In a retrospective review of consecutive patient series conducted in a University level 1 trauma hospital, 43 patients (21 female and eight male), mean age 64.2 (range 16-87) with OTA/AO type B or C pelvic ring fractures received percutaneous pedicle screw and rod internal fixator (INFIX) anterior stabilization of pelvic ring fractures; 29 fulfilled inclusion criteria with three months' minimal follow-up or known complication...
February 24, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28217344/crystal-structure-of-catena-poly-bis-acetato-%C3%AE%C2%BAo-copper-ii-bis-%C3%AE-4-1h-imidazol-1-yl-phenol-%C3%AE%C2%BA-2-n-3-o-%C3%AE%C2%BA-2-o-n-3
#20
Mehmet Poyraz, Musa Sarı
In the title compound, [Cu(CH3COO)2(C9H8N2O)2] n , the Cu(II) ion resides on a centre of inversion, displaying a tetra-gonally distorted octa-hedral coordination environment defined by two pairs of N and O atoms of symmetry-related 4-(1H-imidazol-1-yl)phenol ligands and the O atoms of two symmetry-related acetate ligands. The bridging mode of the 4-(1H-imidazol-1-yl)phenol ligands is associated with a very long Cu⋯O inter-actions involving the phenol O atom of the heterocyclic ligand, which creates chains extending parallel to [100]...
February 1, 2017: Acta Crystallographica. Section E, Crystallographic Communications
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