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https://www.readbyqxmd.com/read/28973904/molecular-architecture-of-the-sheathed-polar-flagellum-in-vibrio-alginolyticus
#1
Shiwei Zhu, Tatsuro Nishikino, Bo Hu, Seiji Kojima, Michio Homma, Jun Liu
Vibrio species are Gram-negative rod-shaped bacteria that are ubiquitous and often highly motile in aqueous environments. Vibrio swimming motility is driven by a polar flagellum covered with a membranous sheath, but this sheathed flagellum is not well understood at the molecular level because of limited structural information. Here, we use Vibrio alginolyticus as a model system to study the sheathed flagellum in intact cells by combining cryoelectron tomography (cryo-ET) and subtomogram analysis with a genetic approach...
September 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28964921/percutaneous-posterior-fixation-for-unstable-pelvic-ring-fractures
#2
C Tempelaere, C Vincent, C Court
INTRODUCTION: Several posterior fixation techniques for unstable pelvic ring fractures have been described. Here we present a minimally invasive, percutaneous technique to fix the two posterior iliac crests using spinal instrumentation. MATERIAL AND METHODS: Between September 2008 and March 2012, 11 patients with a mean age of 36.4 years were operated because of a vertically unstable Tile C pelvic ring fracture. Posterior fixation was performed using two polyaxial screws in each iliac crest with two subfascial connector rods...
September 27, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28963935/sustained-molecular-oxygen-activation-by-solid-iron-doped-silicon-carbide-under-microwave-irradiation-mechanism-and-application-to-norfloxacin-degradation
#3
Hongbo Li, Jing Chen, Huijie Hou, Hong Pan, Xiaoxue Ma, Jiakuan Yang, Linling Wang, John C Crittenden
Sustained molecular oxygen activation by iron doped silicon carbide (Fe/SiC) was investigated under microwave (MW) irradiation. The catalytic performance of Fe/SiC for norfloxacin (NOR) degradation was also studied. Rapid mineralization in neutral solution was observed with a pseudo-first-order rate constant of 0.2239 min(-1) under 540 W of MW irradiation for 20 min. Increasing Fe/SiC rod and MW power significantly enhanced the degradation and mineralization rate with higher yield of reactive oxygen species (ROS)...
September 11, 2017: Water Research
https://www.readbyqxmd.com/read/28961434/photoisomerization-induced-scission-of-rod-like-micelles-unravelled-with-multiscale-modeling
#4
Gabriel Heerdt, Ionut Tranca, Albert J Markvoort, Bartłomiej M Szyja, Nelson H Morgon, Emiel J M Hensen
HYPOTHESIS: In photorheological fluids, subtle molecular changes caused by light lead to abrupt macroscopic alterations. Upon UV irradiation of an aqueous cetyltrimethylammonium bromide (CTAB) and trans-ortho-methoxycinnamic acid (trans-OMCA) solution, for instance, the viscosity drops over orders of magnitude. Multiscale modeling allows to elucidate the mechanisms behind these photorheological effects. EXPERIMENTS: We use time-dependent DFT calculations to study the photoisomerization, and a combination of atomistic molecular dynamics (MD) and DFT to probe the influence of both OMCA isomers on the micellar solutions...
September 12, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28959238/z-ring-structure-and-constriction-dynamics-in-e-coli
#5
Pramod Kumar, Amarjeet Yadav, Itzhak Fishov, Mario Feingold
The Z-ring plays a central role in bacterial division. It consists of FtsZ filaments, but the way these reorganize in the ring-like structure during septation remains largely unknown. Here, we measure the effective constriction dynamics of the ring. Using an oscillating optical trap, we can switch individual rod-shaped E. coli cells between horizontal and vertical orientations. In the vertical orientation, the fluorescent Z-ring image appears as a symmetric circular structure that renders itself to quantitative analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28940520/direction-modulated-brachytherapy-dmbt-for-treatment-of-cervical-cancer-a-planning-study-with-192ir-60co-and-169yb-hdr-sources
#6
Habib Safigholi, Dae Yup Han, Shahram Mashouf, Abraam Soliman, Ali S Meigooni, Amir Owrangi, William Y Song
PURPOSE: To evaluate plan quality of a novel MRI-compatible direction modulated brachytherapy (DMBT) tandem applicator using 192Ir, 60Co, and 169Yb HDR brachytherapy sources, for various cervical cancer high-risk clinical target volumes (CTVHR ). MATERIALS AND METHODS: The novel DMBT tandem applicator has six peripheral grooves of 1.3-mm diameter along a 5.4-mm thick nonmagnetic tungsten alloy rod. Monte Carlo (MC) simulations were used to benchmark the dosimetric parameters of the 192Ir, 60Co, and 169Yb HDR sources in a water phantom against the literature data...
September 22, 2017: Medical Physics
https://www.readbyqxmd.com/read/28910168/reduced-central-retinal-artery-blood-flow-is-related-to-impaired-central-visual-function-in-retinitis-pigmentosa-patients
#7
Samantha Kayser, Patricia Vargas, Deborah Mendelsohn, Jorge Han, Hua Bi, Alexandra Benavente, Ava K Bittner
PURPOSE: We evaluated the test-retest repeatability of blood flow velocities in the retrobulbar central retinal artery (CRA) and explored whether reduced blood flow is related to the degree of visual function loss in retinitis pigmentosa (RP) patients with wide range of disease severity. MATERIALS AND METHODS: We measured CRA peak systolic velocity (PSV) and end diastolic velocity (EDV) to calculate mean flow velocity (MFV) in 18 RP patients using color Doppler imaging with spectral flow Doppler (GE Logiq7 ultrasound) twice in each eye at each of two visits within a month...
September 14, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28885867/clinical-characteristics-of-recessive-retinal-degeneration-due-to-mutations-in-the-cdhr1-gene-and-a-review-of-the-literature
#8
A P Bessette, M J DeBenedictis, E I Traboulsi
BACKGROUND: The clinical phenotype of patients presenting with autosomal recessive CDHR1-related retinopathy has not been well described. MATERIALS AND METHODS: This is a retrospective case series of patients presenting to a single institution. Clinical data, including age, visual acuity, dilated fundus exam, fundus photos, fundus autofluorescence (FAF), optical coherence tomography, full-field electroretinograms (ERGs), and results of genetic testing, were collected...
September 8, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28861775/elastic-and-dielectric-properties-of-ferroelectric-nanoparticles-bent-core-nematic-liquid-crystal-blend
#9
Raj Kumar Khan, Srikanth Turlapati, Nandiraju V S Rao, Sharmistha Ghosh
Bent-core liquid crystals present the first evidence of forming polar superstructures from achiral molecules. The nematic phase is the newest member of the bent-core family and turns out to be extremely interesting owing to its distinct features compared to its calamitic counterpart. Here the investigation of one achiral unsymmetrical 2-methyl-3-amino-benzoic acid (2,6-substituted toluene)-derived four-ring bent-core nematic (BCN) liquid crystals (11-2M-F) is presented after nanodispersion. Ferroelectric nanoparticles significantly affect the phase transition temperature, threshold voltage, dielectric permittivity, elastic constants and splay viscosity of the pristine BCN...
September 2017: European Physical Journal. E, Soft Matter
https://www.readbyqxmd.com/read/28840506/perception-of-the-length-of-an-object-through-dynamic-touch-is-invariant-across-changes-in-the-medium
#10
Madhur Mangalam, Sophie A Barton, Jeffrey B Wagman, Dorothy M Fragaszy, Karl M Newell
Rotational inertia-a mechanical quantity that describes the differential resistance of an object to angular acceleration in different directions-has been shown to support perception of the properties of that object through dynamic touch (wielding). The goal of the present study was to examine if perception of the length of an object through dynamic touch depends on its rotational inertia, independent of the medium in which it is wielded. The participants (n = 14) wielded 12 different objects held in air or completely immersed in water and reported perceived lengths of those objects...
August 24, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/28832104/hybrid-triazine-boron-two-dimensional-covalent-organic-frameworks-synthesis-characterization-and-dft-approach-to-layer-interaction-energies
#11
Krzysztof Gontarczyk, Wojciech Bury, Janusz Serwatowski, Piotr Wieciński, Krzysztof Woźniak, Krzysztof Durka, Sergiusz Luliński
The conversion of 2,4,6-tris(4'-bromophenyl)-1,3,5-triazine to the respective triboronic acid was successfully accomplished by a simple triple Br/Li exchange followed by boronation. Further dehydrative condensation reactions with 2,3,6,7,10,11-hexahydroxytriphenylene or 2,3,6,7-tetrahydroxy-9,10-dilalkylanthracenes (R = Me, Et) resulted in materials featuring good porosity and sorption properties with the nitrogen uptake exceeding 500 cm(3)/g (STP) and SBET up to 1267 m(2)/g (T = 77.2 K). In addition, simple dehydration of this compound was employed for the preparation of a hybrid 2D COF composed of triazine, boroxine, and benzene rings...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28819163/fusion-protein-analysis-reveals-the-precise-regulation-between-hsp70-and-hsp100-during-protein-disaggregation
#12
Sayaka Hayashi, Yosuke Nakazaki, Kei Kagii, Hiromi Imamura, Yo-Hei Watanabe
ClpB, a bacterial Hsp100, is a ring-shaped AAA+ chaperone that can reactivate aggregated proteins in cooperation with DnaK, a bacterial Hsp70, and its co-factors. ClpB subunits comprise two AAA+ modules with an interstitial rod-shaped M-domain. The M-domain regulates ClpB ATPase activity and interacts directly with the DnaK nucleotide-binding domain (NBD). Here, to clarify how these functions contribute to the disaggregation process, we constructed ClpB, DnaK, and aggregated YFP fusion proteins in various combinations...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815296/autoantibodies-against-rods-and-rings-related-impdh2-in-hepatitis-c-genotype-1-and-daa-therapy-in-a-real-life-cohort
#13
Werner Dammermann, Susanne Polywka, Inga Dettmann, Swantje Mindorf, Lars Komorowski, Malte Wehmeyer, Julian Schulze Zur Wiesch, Winfried Stöcker, Stefan Lüth
Autoantibodies against inosine-5'-monophosphate-dehydrogenase-2 (IMPDH2; "rods and rings" pattern) develop in chronic hepatitis C (CHC) patients under treatment with peg-interferon (IFN) and ribavirin (RBV), an inhibitor of IMPDH2. We investigated the influence of the alternative therapy with direct-acting antivirals (DAA)/ribavirin on anti-IMPDH2 autoantibody generation and the use of anti-IMPDH2 development as a marker for therapy outcome (sustained virologic response, SVR). We analyzed a "real life" cohort of 104 unselected CHC genotype 1 (GT1) patients treated with IFN/first-generation DAA/RBV prospectively compared to a historic cohort of 59 IFN/RBV-treated CHC GT1 patients...
August 16, 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28791464/a-computed-tomographic-anatomical-study-of-the-upper-sacrum-application-for-a-user-guide-of-pelvic-fixation-with-iliosacral-screws-in-adult-spinal-deformity
#14
Arnaud Dubory, Houssam Bouloussa, Guillaume Riouallon, Stéphane Wolff
PURPOSE: Widely used in traumatic pelvic ring fractures, the iliosacral (IS) screw technique for spino-pelvic fixation remains anecdotal in adult spinal deformity. The objective of this study was to assess anatomical variability of the adult upper sacrum and to provide a user guide of spino-pelvic fixation with IS screws in adult spinal deformity. METHODS: Anatomical variability of the upper sacrum according to age, gender, height and weight was sought on 30 consecutive pelvic CT-scans...
August 8, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28790240/-successful-treatment-of-acute-empyema-due-to-rupture-of-pyogenic-liver-abscess
#15
Isamu Watanabe, Naoki Kanauchi, Hikaru Watanabe, Akira Hamada
Cases of rescue after rupture of pyogenic liver abscess into the thorax are rare. Here, we report 2 cases of rescue in patients with acute empyema due to rupture of a suppurative abscess into the thorax. Case 1:A 61-year-old male had high fever of 39 °C and right abdominal pain. Thoracic computed tomography(CT) showed encapsulated pleural effusion in the right thorax and ring-like enhancement in the right liver. The diagnosis was acute empyema caused by rupture of liver abscess. The pathogenic bacteria were Streptococcus group...
August 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28766641/complex-self-propelled-rings-a-minimal-model-for-cell-motility
#16
Clara Abaurrea Velasco, Sepehr Dehghani Ghahnaviyeh, Hossein Nejat Pishkenari, Thorsten Auth, Gerhard Gompper
Collective behavior of active matter is observed for self-propelled particles, such as vibrated disks and active Brownian particles, as well as for cytoskeletal filaments in motile cells. Here, a system of quasi two-dimensional penetrable self-propelled rods inside rigid rings is used to construct a complex self-propelled particle. The rods interact sterically with each other and with a stationary or mobile ring via a separation-shifted Lennard-Jones potential. They either have a sliding attachment to the inside of the ring at one of their ends, or can move freely within the ring confinement...
September 13, 2017: Soft Matter
https://www.readbyqxmd.com/read/28723932/the-nucleoid-occlusion-factor-noc-controls-dna-replication-initiation-in-staphylococcus-aureus
#17
Ting Pang, Xindan Wang, Hoong Chuin Lim, Thomas G Bernhardt, David Z Rudner
Successive division events in the spherically shaped bacterium Staphylococcus aureus are oriented in three alternating perpendicular planes. The mechanisms that underlie this relatively unique pattern of division and coordinate it with chromosome segregation remain largely unknown. Thus far, the only known spatial regulator of division in this organism is the nucleoid occlusion protein Noc that inhibits assembly of the cytokinetic ring over the chromosome. However, Noc is not essential in S. aureus, indicating that additional regulators are likely to exist...
July 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28679038/generation-collection-electrochemistry-inside-a-rotating-droplet
#18
Magdalena Kundys, Michal Nejbauer, Martin Jönsson-Niedziolka, Wojciech Adamiak
In this work, we explore generation-collection electrochemistry in a rotating droplet hydrodynamic system, where a 70 μL droplet containing a redox active species (ferrocyanide) is sandwiched between an upper rotating rod and bottom nonmoving generator and collector planar electrodes. In such a system, we studied the effect of the counter electrode reaction on the recorded generator current, and the effect of the generator-collector distance (ranging from 3 to ca. 500 μm) on the collection efficiencies obtained at rotation rates ranging from 50 to 1100 rpm...
July 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28661263/minimally-invasive-treatment-of-unstable-pelvic-ring-injuries-with-modified-pedicle-screw-rod-fixator
#19
Xiao-Tian Wu, Zuo-Qing Liu, Wen-Qin Fu, Shan Zhao
Objective To evaluate the clinical application of the minimally invasive modified pedicle screw-rod fixator for unstable pelvic ring injuries, including its feasibility, merits, and limitations. Methods Twenty-three patients (13 males, 10 females; average age, 36.3 years) with unstable pelvic ring injuries underwent anterior fixation using a modified pedicle screw-rod fixator with or without posterior fixation using a transiliac internal fixator. The clinical findings were assessed using Majeed scores. The quality of reduction was evaluated using the Matta criteria...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28653130/reduction-and-ring-fixation-of-instable-c1-fractures-with-monoaxial-pedicle-screws
#20
Rainer Gumpert, Thomas Poglitsch, Renate Krassnig, Rudolf Pranzl, Paul Puchwein
INTRODUCTION: Ring fixation of C1 can be performed using pedicle screws and a rod in case of unstable Jefferson or lateral mass fractures of C1. MATERIALS AND METHODS: In a case series of three patients, we stabilized C1 fractures surgically using a modified technique of C1 ring fixation by using monoaxial instead of polyaxial screws. Functional outcome and pain was recorded postoperatively. RESULTS: In this very small case series, we observed good results concerning pain and functional outcome...
June 26, 2017: Archives of Orthopaedic and Trauma Surgery
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