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https://www.readbyqxmd.com/read/28723932/the-nucleoid-occlusion-factor-noc-controls-dna-replication-initiation-in-staphylococcus-aureus
#1
Ting Pang, Xindan Wang, Hoong Chuin Lim, Thomas G Bernhardt, David Z Rudner
Successive division events in the spherically shaped bacterium Staphylococcus aureus are oriented in three alternating perpendicular planes. The mechanisms that underlie this relatively unique pattern of division and coordinate it with chromosome segregation remain largely unknown. Thus far, the only known spatial regulator of division in this organism is the nucleoid occlusion protein Noc that inhibits assembly of the cytokinetic ring over the chromosome. However, Noc is not essential in S. aureus, indicating that additional regulators are likely to exist...
July 19, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28679038/generation-collection-electrochemistry-inside-a-rotating-droplet
#2
Magdalena Kundys, Michal Nejbauer, Martin Jönsson-Niedziolka, Wojciech Adamiak
In this work, we explore generation-collection electrochemistry in a rotating droplet hydrodynamic system, where a 70 μL droplet containing a redox active species (ferrocyanide) is sandwiched between an upper rotating rod and bottom nonmoving generator and collector planar electrodes. In such a system, we studied the effect of the counter electrode reaction on the recorded generator current, and the effect of the generator-collector distance (ranging from 3 to ca. 500 μm) on the collection efficiencies obtained at rotation rates ranging from 50 to 1100 rpm...
July 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28661263/minimally-invasive-treatment-of-unstable-pelvic-ring-injuries-with-modified-pedicle-screw-rod-fixator
#3
Xiao-Tian Wu, Zuo-Qing Liu, Wen-Qin Fu, Shan Zhao
Objective To evaluate the clinical application of the minimally invasive modified pedicle screw-rod fixator for unstable pelvic ring injuries, including its feasibility, merits, and limitations. Methods Twenty-three patients (13 males, 10 females; average age, 36.3 years) with unstable pelvic ring injuries underwent anterior fixation using a modified pedicle screw-rod fixator with or without posterior fixation using a transiliac internal fixator. The clinical findings were assessed using Majeed scores. The quality of reduction was evaluated using the Matta criteria...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28653130/reduction-and-ring-fixation-of-instable-c1-fractures-with-monoaxial-pedicle-screws
#4
Rainer Gumpert, Thomas Poglitsch, Renate Krassnig, Rudolf Pranzl, Paul Puchwein
INTRODUCTION: Ring fixation of C1 can be performed using pedicle screws and a rod in case of unstable Jefferson or lateral mass fractures of C1. MATERIALS AND METHODS: In a case series of three patients, we stabilized C1 fractures surgically using a modified technique of C1 ring fixation by using monoaxial instead of polyaxial screws. Functional outcome and pain was recorded postoperatively. RESULTS: In this very small case series, we observed good results concerning pain and functional outcome...
June 26, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28648148/debt-counselling-for-depression-in-primary-care-an-adaptive-randomised-controlled-pilot-trial-decoder-study
#5
Mark B Gabbay, Adele Ring, Richard Byng, Pippa Anderson, Rod S Taylor, Caryn Matthews, Tirril Harris, Vashti Berry, Paula Byrne, Elliot Carter, Pam Clarke, Laura Cocking, Suzanne Edwards, Richard Emsley, Mauro Fornasiero, Lucy Frith, Shaun Harris, Peter Huxley, Siw Jones, Peter Kinderman, Michael King, Liv Kosnes, Daniel Marshall, Dave Mercer, Carl May, Debbie Nolan, Ceri Phillips, Tim Rawcliffe, Alexandra V Sardani, Elizabeth Shaw, Sam Thompson, Jane Vickery, Brian Wainman, Mark Warner
BACKGROUND: Depression and debt are common in the UK. Debt Counselling for Depression in Primary Care: an adaptive randomised controlled pilot trial (DeCoDer) aimed to assess the clinical effectiveness and cost-effectiveness of the addition of a primary care debt counselling advice service to usual care for patients with depression and debt. However, the study was terminated early during the internal pilot trial phase because of recruitment delays. This report describes the rationale, methods and findings of the pilot study, and implications for future research...
June 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28638775/host-guest-complexes-of-c-ethyl-2-methylresorcinarene-and-aromatic-n-n-dioxides
#6
Rakesh Puttreddy, Ngong Kodiah Beyeh, Robin H A Ras, Kari Rissanen
The C-ethyl-2-methylresorcinarene (1) forms 1:1 in-cavity complexes with aromatic N,N'-dioxides, only if each of the aromatic rings has an N-O group. The structurally different C-shaped 2,2'-bipyridine N,N'-dioxide (2,2'-BiPyNO) and the linear rod-shaped 4,4'-bipyridine N,N'-dioxide (4,4'-BiPyNO) both form 1:1 in-cavity complexes with the host resorcinarene in C4v crown and C2v conformations, respectively. In the solid state, the host-guest interactions between the 1,3-bis(4-pyridyl)propane N,N'-dioxide (BiPyPNO) and the host 1 stabilize the unfavorable anti-gauche conformation...
June 2017: ChemistryOpen
https://www.readbyqxmd.com/read/28631069/-s2-ala-iliac-screws-for-extended-pelvic-fixation-in-longer-lumbar-instrumentations-description-of-a-freehand-technique
#7
REVIEW
F Lattig, S Weckbach
OBJECTIVE: To reduce the rate of implant failures at the lumbosacral junction in polysegmental lumbar fusions. INDICATIONS: Spinal fusion with deformity correction including L5/S1, revision surgery for pseudoarthrosis L5/S1, screw pull-out and pedicle fractures in S1, and S1/S2 fractures after polysegmental fusions. CONTRAINDICATIONS: Osteosynthesis of complex pelvic ring fractures, tumors of the ilium. Relative contraindication: infection, to avoid contamination of the iliosacral joint...
June 19, 2017: Operative Orthopädie und Traumatologie
https://www.readbyqxmd.com/read/28587657/modified-pedicle-screw-rod-fixation-as-a-minimally-invasive-treatment-for-anterior-pelvic-ring-injuries-an-initial-case-series
#8
Xiaotian Wu, Zuoqing Liu, Wenqin Fu, Shan Zhao, Juntao Feng
BACKGROUND: Unstable pelvic ring injuries often involve high mortality and morbidity. This study was aimed to evaluate the modified minimally invasive pedicle screw-rod fixation for anterior pelvic ring injuries, in the respects of its feasibility, merits, and limitations. METHODS: Twenty-three patients with unstable pelvic ring injuries underwent the modified anterior pedicle screw-rod fixation, with or without posterior fixation. The clinical outcomes were assessed using Majeed scores, and the quality of reduction was evaluated according to the criteria of Matta...
June 6, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28577249/progeny-production-in-the-periplasm-of-thermosipho-globiformans
#9
Tomohiko Kuwabara, Kensuke Igarashi
Thermotogales are rod-shaped, Gram-negative, anaerobic, (hyper) thermophiles distinguished by an outer sheath-like toga, which comprises an outer membrane (OM) and an amorphous layer (AL). Thermosipho globiformans bacteria can transform into spheroids with multiple cells concurrently with AL disintegration during early growth; the cell is defined as the cytoplasmic membrane (CM) plus the entity surrounded by the CM. Spheroids eventually produce rapidly moving periplasmic 'progenies' through an unknown mechanism...
July 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28552384/light-induced-retinal-degeneration-causes-a-transient-downregulation-of-melanopsin-in-the-rat-retina
#10
Diego García-Ayuso, Caridad Galindo-Romero, Johnny Di Pierdomenico, Manuel Vidal-Sanz, Marta Agudo-Barriuso, María P Villegas Pérez
In this work we study the effects of an acute light-induced retinal degeneration on the population of melanopsin positive retinal ganglion cells (m(+)RGCs) and the expression of the melanopsin protein in the retina. The m(+)RGCs may be more resistant than other RGCs to lesion, but the effects of an acute light exposure in this population are unknown. Albino rats were exposed to white light (3000 lux) continuously for 48 h and processed 0, 3, 7 or 30 days after light exposure (ALE). Whole-mounted retinas were immunodetected with antibodies against melanopsin, Brn3a, and rhodopsin to study the populations of m(+)RGC, Brn3a(+)RGC and rods (which are the most abundant photoreceptors in the rat retina)...
May 26, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28541184/the-step-wise-pathway-of-septin-hetero-octamer-assembly-in-budding-yeast
#11
Andrew Weems, Michael McMurray
Septin proteins bind guanine nucleotides and form rod-shaped hetero-oligomers. Cells choose from a variety of available septins to assemble distinct hetero-oligomers, but the underlying mechanism was unknown. Using a new in vivo assay, we find that a stepwise assembly pathway produces the two species of budding yeast septin hetero-octamers: Cdc11/Shs1-Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Cdc11/Shs1. Rapid GTP hydrolysis by monomeric Cdc10 drives assembly of the core Cdc10 homodimer. The extended Cdc3 N terminus autoinhibits Cdc3 association with Cdc10 homodimers until prior Cdc3-Cdc12 interaction...
May 25, 2017: ELife
https://www.readbyqxmd.com/read/28525300/regulation-of-cell-polarity-in-motility-and-cell-division-in-myxococcus-xanthus
#12
Dominik Schumacher, Lotte Søgaard-Andersen
Rod-shaped Myxococcus xanthus cells are polarized with proteins asymmetrically localizing to specific positions. This spatial organization is important for regulation of motility and cell division and changes over time. Dedicated protein modules regulate motility independent of the cell cycle, and cell division dependent on the cell cycle. For motility, a leading-lagging cell polarity is established that is inverted during cellular reversals. Establishment and inversion of this polarity are regulated hierarchically by interfacing protein modules that sort polarized motility proteins to the correct cell poles or cause their relocation between cell poles during reversals akin to a spatial toggle switch...
May 19, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28516401/endovascular-crossing-of-chronic-total-occlusions-using-an-impulse-an-explorative-design-study
#13
Aimée Sakes, Marleen van der Wiel, Dimitra Dodou, Paul Breedveld
In this study we investigated whether exerting an impulse on a Chronic Total Occlusion (CTO) improves the success rate of CTO crossing as compared to the currently used method of statically pushing the guidewire against the CTO. A prototype (Ø2 mm) was developed that generates translational momentum using a spring-loaded indenter and converts it to an impulse during impact. Mechanical performance was evaluated by measuring the peak force and momentum for different spring compressions and strike distances in air and blood-mimicking fluid...
June 2017: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/28512374/description-of-enchodorus-yeatsi-n-sp-dorylaimida-nordiidae-from-southern-iran-and-its-molecular-phylogenetic-study
#14
Majid Pedram
Enchodorus yeatsi n. sp., a new species of the rare genus Enchodorus is described and illustrated based on its morphological and molecular characters. It was recovered from southern Iran. Females of the new species are characterized by having 1,511.3- to 1,792.5-µm long slender body, angular lip region having prominent papillae, 12- to 13-µm long odontostyle, double guiding ring, simple rod-like odontophore, didelphic-amphidelphic reproductive system, and 102- to 160-µm long elongate-conoid tail with rounded tip...
March 2017: Journal of Nematology
https://www.readbyqxmd.com/read/28500523/cell-cycle-machinery-in-bacillus-subtilis
#15
Jeff Errington, Ling Juan Wu
Bacillus subtilis is the best described member of the Gram positive bacteria. It is a typical rod shaped bacterium and grows by elongation in its long axis, before dividing at mid cell to generate two similar daughter cells. B. subtilis is a particularly interesting model for cell cycle studies because it also carries out a modified, asymmetrical division during endospore formation, which can be simply induced by starvation. Cell growth occurs strictly by elongation of the rod, which maintains a constant diameter at all growth rates...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28487362/complex-binding-pathways-determine-the-regeneration-of-mammalian-green-cone-opsin-with-a-locked-retinal-analogue
#16
Nathan S Alexander, Kota Katayama, Wenyu Sun, David Salom, Sahil Gulati, Jianye Zhang, Muneto Mogi, Krzysztof Palczewski, Beata Jastrzebska
Phototransduction is initiated when the absorption of light converts the 11-cis-retinal chromophore to its all-trans configuration in both rod and cone vertebrate photoreceptors. To sustain vision, 11-cis-retinal is continuously regenerated from its all-trans conformation through a series of enzymatic steps comprising the "visual or retinoid" cycle. Abnormalities in this cycle can compromise vision because of the diminished supply of 11-cis-retinal and the accumulation of toxic, constitutively active opsin...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28486184/bioaccessibility-of-polycyclic-aromatic-hydrocarbons-in-activated-carbon-or-biochar-amended-vegetated-salix-viminalis-soil
#17
Patryk Oleszczuk, Paulina Godlewska, Danny D Reible, Piotr Kraska
The aim of the present study was to determine the effect of activated carbon (AC) or biochars on the bioaccessibility (Cbioacc) of polycyclic aromatic hydrocarbons (PAHs) in soils vegetated with willow (Salix viminalis). The study determined the effect of willow on the Cbioacc PAHs and the effect of the investigated amendments on changes in dissolved organic carbon (DOC), crop yield and the content of PAHs in plants. PAH-contaminated soil was amended with 2.5 wt% AC or biochar. Samples from individual plots with and without plants were collected at the beginning of the experiment and after 3, 6, 12 and 18 months...
August 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28459777/analysis-of-explanted-magnetically-controlled-growing-rods-from-7-uk-spinal-centers
#18
Thomas J Joyce, Simon L Smith, Paul R P Rushton, Andrew J Bowey, Michael J Gibson
STUDY DESIGN: Analysis of explanted MAGnetic Expansion Control (MAGEC) growing rods. OBJECTIVE: To analyze explanted MAGEC rods used in management of early onset scoliosis and identify the mode of failure in such cases. SUMMARY OF BACKGROUND DATA: Magnetically controlled growing rods are increasingly used as the option of choice for early onset scoliosis. However, being more complex than conventional growing rods they are perhaps more likely to succumb to multifarious failure modes...
April 28, 2017: Spine
https://www.readbyqxmd.com/read/28453390/the-torments-of-the-cohesin-ring
#19
Alap P Chavda, Keven Ang, Dmitri Ivanov
Cohesin is a ring-shaped protein complex which comprises the Smc1, Smc3 and Scc1 subunits. It topologically embraces chromosomal DNA to connect sister chromatids and stabilize chromatin loops. It is required for proper chromosomal segregation, DNA repair and transcriptional regulation. We have recently reported that cohesin rings can adopt a "collapsed" rod-like conformation which is driven by the interaction between the Smc1 and Smc3 coiled coil arms and is regulated by post-translational modifications. The "collapsed" conformation plays a role in cohesin ring assembly and its loading on the DNA...
May 4, 2017: Nucleus
https://www.readbyqxmd.com/read/28449859/the-role-of-the-intramedullary-implant-in-limb-lengthening
#20
Peter R Calder, Maritz Laubscher, W David Goodier
Limb lengthening is now an accepted practice in orthopaedic surgery. The principles of distraction osteogenesis have become well established with the use of external fixators, utilizing both monolateral and ring fixators. Corticotomy technique, frame stability, lengthening rate and rhythm all contribute to the formation of bone regenerate and tissues. Complications are however common including pin-site infection, soft tissue tethering from the pins and wires resulting in pain, regenerate deformity from soft tissue forces or fracture following frame removal and patient intolerance of the frames during treatment...
April 24, 2017: Injury
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