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tissue cross reactivity

Karen K Szumlinski, Alexis W Ary, Christina B Shin, Melissa G Wroten, Justin Courson, Bailey W Miller, Micaela Ruppert-Majer, John W Hiller, John R Shahin, Osnat Ben-Shahar, Tod E Kippin
Phosphatidylinositide 3-kinases (PI3Ks) are intracellular signal transducer enzymes that recruit protein kinase B (aka Akt) to the cell membrane, the subsequent activation of which regulates many cellular functions. PI3K/Akt activity is up-regulated within mesocorticolimbic structures in animal models of alcoholism, but less is known regarding PI3K/Akt activity in animal models of cocaine addiction. Given that prefrontal cortex (PFC) is grossly dysregulated in addiction, we studied how cocaine affects protein indices of PFC PI3K/Akt activity in rat and mouse models and examined the relevance of PI3K activity for cocaine-related learning...
November 18, 2018: Addiction Biology
Qingli Zhang, Megan Shavalier, Isaac Standish, Gavin W Glenney, Thomas P Loch, Mohamed Faisal
Epizootic Epitheliotropic Disease Virus (EEDV; Salmonid Herpesvirus-3) causes a serious disease hatchery-reared lake trout (Salvelinus namaycush), threatening restoration efforts of this species in North America. The current inability to replicate EEDV in vitro necessitates the search for a reproducible, sensitive, and specific assay that allows for its detection and quantitation in a time- and cost-effective manner. Herein, we describe a loop-mediated isothermal amplification (LAMP) assay that was developed for the quantitative detection of EEDV in infected fish tissues...
November 13, 2018: Journal of Virological Methods
Hongchae Baek, Anvar Sariev, Min-Ju Kim, Hojin Lee, Jinhyun Kim, Hyungmin Kim
The role of established contralateral cerebrocerebellar connections on cerebellar injury during stroke has been increasingly revealed in recent years. An extensive number of studies have investigated alteration in inter-hemispheric correlation in order to find brain regions whose responses are specific to restore functional loss and enhance adaptive neural plasticity after stroke. Although, several non-invasive brain stimulation studies have proven their efficacy in the treatment of stroke recovery, finding more effective brain regions that responsible for stroke rehabilitation as well as optimizing neural stimulation protocol are the main goals of further investigations...
July 2018: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Silvana Castelli, Iride Mascheretti, Cristian Cosentino, Barbara Lazzari, Raul Pirona, Aldo Ceriotti, Angelo Viotti, Massimiliano Lauria
The α-zein gene family encodes the most abundant storage proteins of maize (Zea mays) endosperm. Members of this family are expressed in a parent-of-origin manner. To characterize this phenomenon further, we investigated the expression of a subset of α-zein polypeptides in reciprocal crosses between o2 lines that were characterized by a simplified α-zein pattern. Maize lines that suppressed the expression of α-zeins when used as female parents were identified. The suppression was cross-specific, occurring only when specific genetic backgrounds were combined...
2018: PloS One
Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll
Transglutaminase (TGase) is a Ca2+-dependent cross-linking enzyme, which has both enzymatic and non-enzymatic properties. TGase is involved in several cellular activities, including adhesion, migration, survival, apoptosis, and extracellular matrix (ECM) organization. In this study, we focused on the role of the TGase enzyme in controlling hematopoiesis in the crayfish Pacifastacus leniusculus. We hypothesized that a high TGase activity could mediate an interaction of progenitor cells with the ECM to maintain cells in an undifferentiated stage in the hematopoietic tissue (HPT)...
November 13, 2018: Journal of Biological Chemistry
Mary M Torregrossa, Matthew MacDonald, Kathryn L Stone, TuKiet T Lam, Angus C Nairn, Jane R Taylor
RATIONALE: Environmental stimuli, or cues, associated with the use of drugs such as cocaine are one of the primary drivers of relapse. Thus, identifying mechanisms to reduce the motivational properties of drug cues is an important research goal. OBJECTIVES: The purpose of this study was to identify cellular signaling events in the nucleus accumbens (NAc) that are induced when a cocaine cue memory is either extinguished through repeated cue presentation in the absence of drug, or when the memory is reactivated and reconsolidated by a brief cue re-exposure...
November 8, 2018: Psychopharmacology
Veronika Vidova, Eliska Stuchlikova, Marketa Vrbova, Martina Almasi, Jana Klánová, Vojtech Thon, Zdenek Spacil
Inflammation is the first line defense mechanism against infection, tissue damage or cancer development. However, inappropriate inflammatory response may also trigger diseases. The quantification of inflammatory proteins is essential to distinguish between harmful and beneficial immune response. Currently used immunoanalytical assays may suffer specificity issues due to antigen-antibody interaction and possible cross-reactivity of antibody with other protein species. In addition, immunoanalytical assays typically require invasive blood sampling and additional logistics; they are relatively costly and highly challenging to multiplex...
November 8, 2018: Journal of Proteome Research
Nicholas N DePhillipo, Zachary S Aman, Mitchell I Kennedy, J P Begley, Gilbert Moatshe, Robert F LaPrade
Background: Recent investigations on the biochemical pathways after a musculoskeletal injury have suggested that vitamin C (ascorbic acid) may be a viable supplement to enhance collagen synthesis and soft tissue healing. Purpose: To (1) summarize vitamin C treatment protocols; (2) report on the efficacy of vitamin C in accelerating healing after bone, tendon, and ligament injuries in vivo and in vitro; and (3) report on the efficacy of vitamin C as an antioxidant protecting against fibrosis and promoting collagen synthesis...
October 2018: Orthopaedic Journal of Sports Medicine
Catalina Salgado-Salazar, Gary R Bauchan, Emma C Wallace, Jo Anne Crouch
Background: Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic detection of P. obducens from leaves of impatiens, which may be contributing to the spread of the disease. Fluorescence in situ hybridization (FISH) is a sensitive and robust method that uses sequence-specific, fluorescence-labeled oligonucleotide probes to detect target organisms from the environment...
2018: Plant Methods
Naomi R Ondrasek, Sara M Freeman, Karen L Bales, Rebecca M Calisi
Collective behaviors, including flocking and group vocalizing, are readily observable across a diversity of free-living avian populations, yet we know little about how neural and ecological factors interactively regulate these behaviors. Because of their involvement in mediating a variety of social behaviors, including avian flocking, nonapeptides are likely mediators of collective behaviors. To advance the neuroecological study of collective behaviors in birds, we sought to map the neuroanatomical distributions of nonapeptide receptors in three promising avian models that are found across a diversity of environments and widely ranging ecological conditions: European starlings, house sparrows, and rock doves...
2018: Frontiers in Neuroscience
Zhou Zhang, Yongning Zhang, Lin Xiangmei, Zhenhai Chen, Wu Shaoqiang
In pigs, Senecavirus A (SVA) causes a vesicular disease that is clinically indistinguishable from foot-and-mouth disease, vesicular stomatitis and swine vesicular disease. Sensitive and specific detection of SVA is critical for controlling this emerging disease. In this study, a novel reverse transcription droplet digital PCR (RT-ddPCR) assay, targeting the conserved viral polymerase 3D gene, was established for the detection of SVA. This assay exhibited good linearity, repeatability and reproducibility, and maintained linearity at extremely low concentrations of SVA nucleic acid templates...
October 30, 2018: Transboundary and Emerging Diseases
Mingwang Yang, Jiangli Fan, Jianjun Du, Saran Long, Jia Wang, Xiaojun Peng
Formaldehyde (FA), a highly reactive carbonyl species, plays significant role in physiological and pathological functions. However, elevated FA will lead to cognitive impairments, memory loss and various neurodegenerative diseases due to its potent DNA and protein cross-linking mechanisms. In this work, a fluorescence probe, BD-CHO , based on benz-2-oxa-1, 3- diazole ( BD ) skeleton, was designed and synthesized for detection of FA via Aza-Cope reaction with high selectivity and large Stokes shifts (about 118 nm)...
2018: Frontiers in Chemistry
Rupnarayan Bhattacharya, Piyali Das, Siddhartha Narayan Joardar, Bikash Kanti Biswas, Subhasis Batabyal, Pradip Kumar Das, Samit Kumar Nandi
Restoration of the external ear and nose in human patients, in either congenital deformity or acquired defects, is a challenge in reconstructive surgery. Optimization of the currently available materials is necessary for rhinoplasty and microtia correction to avoid intraoperative manoeuvring and early rejection. The aim of this study is to develop cross-linked decellularized caprine conchal cartilages as biocompatible, robust and non-toxic matrix template. The characterization of the decellularized tissue encompasses in vitro lymphoproliferation assay, cytotoxicity test, agar gel precipitation test, in vivo immunocompatibility study, histology, and determination of pro-inflammatory cytokines in animal model...
October 24, 2018: Journal of Tissue Engineering and Regenerative Medicine
Heidar Ali Panahi, Azam Bolhassani, Gholamreza Javadi, Zahra Noormohammadi
Human papillomaviruses (HPVs) are a group of circular double-stranded DNA viruses, showing severe tropism to mucosal tissues. A subset of HPVs, especially HPV16 and 18, are the primary etiological cause for several epithelial cell malignancies, causing about 5.2% of all cancers worldwide. Due to the high prevalence and mortality, HPV-associated cancers have remained as a significant health problem in human society, making an urgent need to develop an effective therapeutic vaccine against them. Achieving this goal is primarily dependent on the identification of efficient tumor-associated epitopes, inducing a robust cell-mediated immune response...
2018: PloS One
Yoko Nagai, Masataka Oitate, Hideyuki Shiozawa, Osamu Ando
1. Trastuzumab deruxtecan (DS-8201a) is an antibody-drug conjugate (ADC) composed of a monoclonal antibody targeting human epidermal growth factor receptor 2 (HER2) conjugated to a topoisomerase I inhibitor (DXd) at a drug-to-antibody ratio (DAR) of 7-8. Here, we examined the pharmacokinetic (PK) profiles of DS-8201a and DXd in cynomolgus monkeys, a cross-reactive species. 2. Following intravenous (iv) administration of DS-8201a, the linker was stable in plasma, and systemic DXd exposure was low. DXd was rapidly cleared following iv dosing...
October 23, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
M Karademir, M A Sonmez, R Akcilar, E Kocak, A Yay, O Eser
OBJECTIVE: This study aimed to investigate the therapeutic effect of ozone in combination with insulin on cranial and spinal neuropathy in rats with diabetes mellitus (DM). MATERIALS AND METHODS: Sixty adult male Sprague Dawley rats were randomly divided into the following six groups (n = 10): control (C), ozone (O), diabetic (D), ozone-treated diabetic (DO), insulin-treated diabetic (DI), and ozone, insulin-treated diabetic (DOI). DM was induced by a single intraperitoneal (ip) streptozotocin injection (60 mg/kg), followed by 3 IU (ip) insulin administration for 60 days...
2018: Bratislavské Lekárske Listy
Ujwal Bhure, Justus E Roos, Klaus Strobel
Osteoid osteoma is a painful, benign, osteoblastic lesion that occurs in younger patients and affects the extremities or the axial skeleton. While plain film findings may suggest the diagnosis, in complex anatomical regions such as the spine, pelvis, wrist and foot advanced imaging modalities are often required. A typical nidus surrounded by sclerosis or cortical thickening characterizes osteoid osteoma on plain radiography and CT. MR is the cross-sectional imaging modality of choice for most musculoskeletal disorders...
October 19, 2018: European Journal of Nuclear Medicine and Molecular Imaging
Annacarmen Petrizzo, Maria Tagliamonte, Angela Mauriello, Valerio Costa, Marianna Aprile, Roberta Esposito, Andrea Caporale, Antonio Luciano, Claudio Arra, Maria Lina Tornesello, Franco M Buonaguro, Luigi Buonaguro
BACKGROUND: A novel prediction algorithm is needed for the identification of effective tumor associated mutated neoantigens. Only those with no homology to self wild type antigens are true predicted neoantigens (TPNAs) and can elicit an antitumor T cell response, not attenuated by central tolerance. To this aim, the mutational landscape was evaluated in HCV-associated hepatocellular carcinoma. METHODS: Liver tumor biopsies and adjacent non-tumor liver tissues were obtained from 9 HCV-chronically infected subjects and subjected to RNA-Seq analysis...
October 19, 2018: Journal of Translational Medicine
Jie-Hua Chen, Xu Lin, Cuihong Bu, Xuguang Zhang
Advanced glycation end products (AGEs), a group of compounds that are formed by non-enzymatic reactions between carbonyl groups of reducing sugars and free amino groups of proteins, lipids or nucleic acids, can be obtained exogenously from diet or formed endogenously within the body. AGEs accumulate intracellularly and extracellularly in all tissues and body fluids and can cross-link with other proteins and thus affect their normal functions. Furthermore, AGEs can interact with specific cell surface receptors and hence alter cell intracellular signaling, gene expression, the production of reactive oxygen species and the activation of several inflammatory pathways...
2018: Nutrition & Metabolism
Liang-Lin Qiu, Chao Wang, Shen Yao, Na Li, Yuhuan Hu, Yongquan Yu, Rong Xia, Jiansheng Zhu, Minghui Ji, Zhan Zhang, Shou-Lin Wang
Fenvalerate (FEN), a mainstream pyrethroid pesticide, was initially recommended as a low-toxicity agent for controlling agricultural and domestic pests. Despite the widespread use of FEN worldwide, little data are available on FEN-induced hepatic lesions and molecular mechanisms. In the present study, we first performed an occupational cross-sectional study on FEN factory workers and found that the levels of serum alanine aminotransferase (ALT) and total antioxidant capacity increased, whereas malondialdehyde decreased in laborers in the working areas where the levels of airborne FEN were much higher compared with the office area...
October 11, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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