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tissue cross reactivity

Anke Schlüter, Anja K Eckstein, Alexandra Brenzel, Mareike Horstmann, Stephan Lang, Utta Berchner-Pfannschmidt, J Paul Banga, Salvador Diaz-Cano
Objectives: Experimental models of Graves hyperthyroid disease accompanied by Graves orbitopathy (GO) can be efficiently induced in susceptible inbred strains of mice by immunization by electroporation of heterologous human TSH receptor (TSHR) A-subunit plasmid. The interrelated pathological findings in the thyroid glands of Graves disease (GD) that explain the core changes classically include diffuse follicular hyperplasia and multifocal mild lymphocytic infiltrate. However, the relative contributions of different thyroid tissue components (colloid, follicular cells, and stroma) have not been previously evaluated...
June 2018: European Thyroid Journal
Chun-Heng Kuo, Jung-Nan Wei, Chung-Yi Yang, Horng-Yih Ou, Hung-Tsung Wu, Kang-Chih Fan, Shu-Huei Wang, Cyue-Huei Hua, Ching-Hsiang Hsiao, Mei-Kuei Lee, Hung-Yuan Li
BACKGROUND/OBJECTIVES: Vascular adhesion protein-1 (VAP-1) can enhance tissue glucose uptake in cell studies and normalize hyperglycemia in animal studies. However, serum VAP-1 concentration (sVAP-1) is higher in subjects with diabetes in cross-sectional studies. In this cohort study, we test our hypothesis that sVAP-1 is increased in prediabetes to counteract hyperglycemia and is associated with incident diabetes negatively. SUBJECTS/METHODS: From 2006 to 2012, 600 subjects without diabetes from Taiwan Lifestyle Study were included and followed regularly...
July 18, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Cristina Anna Gallelli, Silvio Calcagnini, Adele Romano, Justyna Barbara Koczwara, Marialuisa de Ceglia, Donatella Dante, Rosanna Villani, Anna Maria Giudetti, Tommaso Cassano, Silvana Gaetani
Growing evidence supports the pivotal role played by oxidative stress in tissue injury development, thus resulting in several pathologies including cardiovascular, renal, neuropsychiatric, and neurodegenerative disorders, all characterized by an altered oxidative status. Reactive oxygen and nitrogen species and lipid peroxidation-derived reactive aldehydes including acrolein, malondialdehyde, and 4-hydroxy-2-nonenal, among others, are the main responsible for cellular and tissue damages occurring in redox-dependent processes...
July 18, 2018: Antioxidants (Basel, Switzerland)
Xinyan Bi, Yi Ting Loo, Shalini D/O Ponnalagu, Christiani Jeyakumar Henry
BACKGROUND AND AIMS: High-sensitivity C-reactive protein (hs CRP) has emerged as an inflammatory biomarker to predict metabolic syndrome. Here, we investigate the association of hs CRP with metabolic variables and determine the risks for elevated hs CRP levels in healthy Singaporean adults. METHODS: We conducted a cross-sectional study of 225 participants (104 men). The levels of hs CRP and fasting lipid parameters were analyzed by COBAS. Body composition was determined with dual-energy X-ray absorptiometry...
July 17, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Melania Spadaro, Stephan Winklmeier, Eduardo Beltrán, Caterina Macrini, Romana Höftberger, Elisabeth Schuh, Franziska S Thaler, Lisa-Ann Gerdes, Sarah Laurent, Ramona Gerhards, Simone Brändle, Klaus Dornmair, Constanze Breithaupt, Markus Krumbholz, Markus Moser, Gurumoorthy Kirshnamoorthy, Frits Kamp, Dieter Jenne, Reinhard Hohlfeld, Tania Kümpfel, Hans Lassmann, Naoto Kawakami, Edgar Meinl
OBJECTIVE: Autoantibodies against myelin oligodendrocyte glycoprotein (MOG) occur in a proportion of patients with inflammatory demyelinating diseases of the CNS. We analyzed their pathogenic activity by affinity-purifying these Abs from patients and transferring them to experimental animals. METHODS: Patients with Abs to MOG were identified by cell-based assay. We determined the cross-reactivity to rodent MOG and determined the recognized MOG-epitopes. We produced the correctly folded extracellular domain of MOG and affinity-purified MOG-specific Abs from the blood of patients...
July 16, 2018: Annals of Neurology
Ahreum Lee, Yong C Kim, Keumjin Baek, Jehan Alam, Yun S Choi, Yaeeun Rheu, Yoo Jin Shin, Sungtae Kim, Hyun-Duck Kim, Yeong W Song, Youngnim Choi
Autoantibodies against alpha-enolase (ENO1) are often detected in various infectious and autoimmune diseases. Anti-ENO1 antibody titers were reported to be associated with the severity of periodontitis in patients with rheumatoid arthritis. Because the enolase of the periodontal pathogen Treponema denticola (TdEno) has the highest homology with ENO1 among the enolases of human-associated bacteria, we hypothesized that anti-ENO1 autoantibodies produced during the immune response to TdEno may contribute to the progression of periodontitis and tested it in human and mouse systems...
July 12, 2018: Virulence
Anton Gisterå, Maria L Klement, Konstantinos A Polyzos, Reiner K Mailer, Amanda Duhlin, Mikael C I Karlsson, Daniel F J Ketelhuth, Göran K Hansson
Background -Atherosclerotic cardiovascular disease is a chronic inflammatory process initiated when cholesterol-carrying low-density lipoprotein (LDL) is retained in the arterial wall. CD4+ T cells, some of which recognize peptide components of LDL as antigen, are recruited to the forming lesion, resulting in T-cell activation. Although these T cells are thought to be proatherogenic, LDL immunization reduces disease in experimental animals. These seemingly contradictory findings have hampered the development of immune-based cardiovascular therapy...
July 11, 2018: Circulation
Dong Sun Kim, Eun Jeong Ok, Beom Hee Choi, Nam-Seok Joo
Background: Metabolic syndrome (MS) is a well-known risk factor of cardiovascular diseases that is focused on central obesity. Recent studies have reported the association between pericardial adipose tissue (PAT) volume and MS. However, no studies have demonstrated the cutoff PAT volume that represents the best association with MS. Methods: The data of 374 subjects were analyzed cross-sectionally to compare PAT, measured on coronary multidetector computed tomography, and various metabolic parameters according to MS...
July 9, 2018: Korean Journal of Family Medicine
Damariz Marín-Palma, Gustavo A Castro, Jaiberth A Cardona-Arias, Silvio Urcuqui-Inchima, Juan C Hernandez
Introduction: High-density lipoproteins (HDL) are responsible for the efflux and transport of cholesterol from peripheral tissues to the liver. In addition, HDL can modulate various immunological mechanisms, including the inflammatory response. Inflammasomes are multiprotein complexes that have been reported to be activated during human immunodeficiency virus type 1 (HIV-1) infection, thus contributing to immune hyperactivation, which is the main pathogenic mechanism of HIV-1 progression...
2018: Frontiers in Immunology
Qunqun Bao, Ping Hu, Yingying Xu, Tiansheng Cheng, Chenyang Wei, Limin Pan, Jianlin Shi
Cerebral vasculature and neuronal networks will be largely destroyed due to the oxidative damages by over-produced reactive oxygen species (ROS) during stroke, accompanied by the symptoms of ischemic injury and blood-brain barrier (BBB) disruption. Ceria nanoparticles, acting as an effective and recyclable ROS scavenger, have been showing high effectiveness in neuroprotection. However, the brain access of nanoparticles can only be achieved by targeting the damaged area of BBB, leading to the disrupted BBB unprotected and turbulence of microenvironment in brain...
June 22, 2018: ACS Nano
Akihiro Kudoh, Hiroaki Satoh, Hiroyuki Hirai, Tsuyoshi Watanabe, Michio Shimabukuro
The cross talk between the adipose tissue and insulin target tissues is a key mechanism for obesity-associated insulin resistance. However, the precise role of the interaction between the skeletal muscle and adipose tissue for insulin signaling and glucose uptake is questionable. L6 myocytes were co-cultured with or without 3T3-L1 adipocytes (~5 × 103 cells/cm2 ) up to 24 h. Glucose uptake was evaluated by 2-[3 H] deoxyglucose uptake assay. Levels of mRNA expression of Glut1 and Glut4 and mitochondrial enzymes were analyzed by quantitative real-time reverse transcription polymerase chain reaction...
2018: Frontiers in Endocrinology
Vardaman M Buckalew
Endogenous digitalis-like factor(s), originally proposed as a vasoconstrictor natriuretic hormone, was discovered in fetal and neonatal blood accidentally because it cross-reacts with antidigoxin antibodies (ADAs). Early studies using immunoassays with ADA identified the digoxin-like immuno-reactive factor(s) (EDLF) in maternal blood as well, and suggested it originated in the feto-placental unit. Mammalian digoxin-like factors have recently been identified as at least two classes of steroid compounds, plant derived ouabain (O), and several toad derived bufodienolides, most prominent being marinobufagenin (MBG)...
June 29, 2018: Clinical Science (1979-)
Kübra Can, Uğur Aksu, Osman Şadi Yenen
Background/aim: The aim of this study was to investigate the effects of PhiKZ phage therapy and meropenem alone or combined treatments in a pneumonia mouse model induced by the Pseudomonas aeruginosa PAO1 strain. The cross-talk between lungs and kidneys was also determined. Materials and methods: The systemic, lung-specific, and kidney-specific inflammation levels and the bacterial load in lung tissue and biochemical parameters were investigated after PhiKZ phage therapy and meropenem alone or combined treatments in a pneumonia mouse model induced by the P...
June 14, 2018: Turkish Journal of Medical Sciences
Yuyuan Zhang, Xue Chen, Yun Wang, Chunhua Wang
OBJECTIVE: To investigate the oxidative damage induced by cadmium choride and the expression of nuclear factor κB( NF-κB), tumor necrosis factor-α( TNF-α) and intercellular cell adhesion molecule-1( ICAM-1) in brain tissue of mice. METHODS: A total of 32 adult ICR mice were divided into 4 groups randomly including CdCl_2 2. 0, 1. 0 and 0. 5 mg/kg and NS control group, all mice were through intraperitoneally injected administration for 14 days, after the last exposure, open-field test were used and all mice were sacrificed by cervical dislocation, the expression of reactive oxygen species( ROS)was measured and the expression of TNF-α, ICAM-1 were observed by ELISA, the level of NF-κB P65 in the brain were detected by immunohistochemistry and western blot, the slices of the brain were stained with HE for histopathological examination...
September 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
Dan Li, Lei Wang, Brandon F Maziuk, Xudong Yao, Benjamin Wolozin, Yong Ku Cho
Antibodies are essential biochemical reagents for detecting protein post-translational modifications (PTMs) in complex samples. However, recent efforts in developing PTM-targeting antibodies have reported frequent non-specific binding and limited affinity of such antibodies. To address these challenges, we investigated whether directed evolution could be applied to improve the affinity of a high-specificity antibody targeting phospho-threonine 231 (pT231) of the human microtubule-associated protein tau. On the basis of existing structural information, we hypothesized that improving antibody affinity may come at the cost of loss in specificity...
June 13, 2018: Journal of Biological Chemistry
Sathyadeepak Ramesh, Alexander Nobori, Yi Wang, Daniel Rootman, Robert Alan Goldberg
PURPOSE: To test the hypothesis that the orbit expands in the cranial vault after wide dural exposure after minimally invasive extradural transorbital decompression for thyroid eye disease. METHODS: A cross-sectional cohort study of 36 patients (60 orbits). Preoperative and postoperative (6 months) orbital CT following extradural transorbital decompression was analyzed. Primary outcome measure was the percentage area of the cranial vault occupied by orbital and brain tissue in a predefined window before and after surgery...
June 6, 2018: Ophthalmic Plastic and Reconstructive Surgery
Peilin Zhang, Lawrence M Minardi, J Todd Kuenstner, Steven M Zekan, Rusty Kruzelock
Autoimmune diseases are a spectrum of clinical inflammatory syndromes with circulating autoantibodies. Autoimmune diseases affect millions of patients worldwide with enormous costs to patients and society. The diagnosis of autoimmune diseases relies on the presence of autoantibodies and the treatment strategy is to suppress the immune system using specific or non-specific immunosuppressive agents. The discovery of anti-microbial antibodies in the blood of patients with Crohn's disease and Sjogren's syndrome and cross-reactivity of anti-microbial antibodies to human tissue suggests a new molecular mechanism of pathogenesis, raising the possibility of designing a new therapeutic strategy for these patients...
2018: Frontiers in Medicine
Nirmalya Sen, Allison M Cross, Philip L Lorenzi, Javed Khan, Berkley E Gryder, Suntae Kim, Natasha J Caplen
Ewing sarcoma (EWS) is a soft tissue and bone tumor that occurs primarily in adolescents and young adults. In most cases of EWS, the chimeric transcription factor, EWS-FLI1 is the primary oncogenic driver. The epigenome of EWS cells reflects EWS-FLI1 binding and activation or repression of transcription. Here, we demonstrate that EWS-FLI1 positively regulates the expression of proteins required for serine-glycine biosynthesis and uptake of the alternative nutrient source glutamine. Specifically, we show that EWS-FLI1 activates expression of PHGDH, PSAT1, PSPH, and SHMT2...
June 6, 2018: Molecular Carcinogenesis
Huihong Zeng, Gayani K Nanayakkara, Ying Shao, Hangfei Fu, Yu Sun, Ramon Cueto, William Y Yang, Qian Yang, Haitao Sheng, Na Wu, Luqiao Wang, Wuping Yang, Hongping Chen, Lijian Shao, Jianxin Sun, Xuebin Qin, Joon Y Park, Konstantinos Drosatos, Eric T Choi, Qingxian Zhu, Hong Wang, Xiaofeng Yang
Under inflammatory conditions, inflammatory cells release reactive oxygen species (ROS) and reactive nitrogen species (RNS) which cause DNA damage. If not appropriately repaired, DNA damage leads to gene mutations and genomic instability. DNA damage checkpoint factors (DDCF) and DNA damage repair factors (DDRF) play a vital role in maintaining genomic integrity. However, how DDCFs and DDRFs are modulated under physiological and pathological conditions are not fully known. We took an experimental database analysis to determine the expression of 26 DNA DD C Fs and 42 DNA DD R Fs in 21 human and 20 mouse tissues in physiological/pathological conditions...
2018: Frontiers in Physiology
Jacqueline Ho, Aneeza W Hamizan, Raquel Alvarado, Janet Rimmer, William A Sewell, Richard J Harvey
Background Eosinophilic chronic rhinosinusitis (eCRS) is linked with skewed T-helper 2 or immunoglobulin E (IgE)-mediated allergic responses, with differing diagnosis, prognosis, and management to non-eCRS. Objective The association between biomarkers and eCRS was investigated to assess the predictors of eCRS. Methods A cross-sectional study of adult patients with chronic rhinosinusitis (CRS) undergoing endoscopic sinus surgery was conducted. eCRS was defined by histopathological assessment showing >10 eosinophils/high-power field on sinus mucosal biopsy...
January 1, 2018: American Journal of Rhinology & Allergy
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