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https://www.readbyqxmd.com/read/28207828/the-wide-spectrum-high-biocidal-potency-of-bioxy-formulation-when-dissolved-in-water-at-different-concentrations
#1
Dori Dagher, Ken Ungar, Richard Robison, Fadi Dagher
Traditional surface disinfectants that have long been applied in medicine, animal husbandry, manufacturing and institutions are inconvenient at best and dangerous at worst. Moreover, some of these substances have adverse environmental impacts: for example, quaternary ammonium compounds ("quats") are reproductive toxicants in both fish and mammals. Halogens are corrosive both to metals and living tissues, are highly reactive, can be readily neutralized by metals, and react with organic matter to form toxic, persistent by-products such as dioxins and furans...
2017: PloS One
https://www.readbyqxmd.com/read/28202751/superiority-in-rhesus-macaques-of-targeting-hiv-1-env-gp140-to-cd40-versus-lox-1-in-combination-with-replication-competent-nyvac-kc-for-induction-of-env-specific-antibody-and-t-cell-responses
#2
Gerard Zurawski, Xiaoying Shen, Sandra Zurawski, Georgia D Tomaras, David C Montefiori, Mario Roederer, Guido Ferrari, Christine Lacabaratz, Peter Klucar, Zhiqing Wang, Kathryn E Foulds, Shing-Fen Kao, Xuesong Yu, Alicia Sato, Nicole L Yates, Celia LaBranche, Sherry Stanfield-Oakley, Karen Kibler, Bertram Jacobs, Andres Salazar, Steve Self, Jimmy Fulp, Raphael Gottardo, Lindsey Galmin, Deborah Weiss, Anthony Cristillo, Giuseppe Pantaleo, Yves Levy
We compared the HIV-1-specific immune responses generated by targeting HIV-1 envelope protein (Env gp140) to either CD40 or LOX-1, two endocytic receptors on dendritic cells (DCs), in Rhesus macaques primed with a poxvirus vector (NYVAC-KC) expressing Env gp140. The DC-targeting vaccines, humanized recombinant monoclonal antibodies fused to Env gp140, were administered as a boost with poly ICLC adjuvant either alone or co-administered with the NYVAC-KC vector. All the DC-targeting vaccine administrations with poly ICLC increased the low-level serum anti-Env IgG responses elicited by NYVAC-KC priming significantly more (up to P =0...
February 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28194371/non-tuberculous-mycobacteria-in-south-african-wildlife-neglected-pathogens-and-potential-impediments-for-bovine-tuberculosis-diagnosis
#3
Nomakorinte Gcebe, Tiny M Hlokwe
Non-tuberculous mycobacteria (NTM) are not only emerging and opportunistic pathogens of both humans and animals, but from a veterinary point of view some species induce cross-reactive immune responses that hamper the diagnosis of bovine tuberculosis (bTB) in both livestock and wildlife. Little information is available about NTM species circulating in wildlife species of South Africa. In this study, we determined the diversity of NTM isolated from wildlife species from South Africa as well as Botswana. Thirty known NTM species and subspecies, as well as unidentified NTM, and NTM closely related to Mycobacterium goodii/Mycobacterium smegmatis were identified from 102 isolates cultured between the years 1998 and 2010, using a combination of molecular assays viz PCR and sequencing of different Mycobacterial house-keeping genes as well as single nucleotide polymorphism (SNP) analysis...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28192925/two-photon-ratiometric-fluorescence-probe-with-enhanced-absorption-cross-section-for-imaging-and-biosensing-of-zinc-ions-in-hippocampal-tissue-and-zebrafish
#4
Wanying Li, Bingqing Fang, Ming Jin, Yang Tian
Zinc ion (Zn(2+)) not only plays an important function in the structural, catalytic, transcription, and regulatory of proteins, but is also an essential ionic signal to regulate brain neurotransmitters pass process. In this work, we designed and synthesized an intramolecular charge transfer-based ratiometric two-photon fluorescence probe, P-Zn, for imaging and biosensing of Zn(2+) in live cell, hippocampal tissue, and zebrafish. The developed probe demonstrated high two-photon absorption cross section (δ) of 516 ± 77 GM, which increased to 958 ± 144 GM after the probe was coordinated with Zn(2+)...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28185188/hapten-anti-hapten-technique-for-two-color-ihc-detection-of-phosphorylated-egfr-and-h2ax-using-primary-antibodies-raised-in-the-same-host-species
#5
Jodi Hagen, David Schwartz, Alexander E Kalyuzhny
Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant primary antibodies. Several techniques can be used to overcome this obstacle including direct labeling of primary antibodies with fluorescent tags and using tyramide signal amplification. Unfortunately these techniques either lack sensitivity, or require a long multistep protocol which can cause physical damage of specimens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28184946/uptake-of-111in-labeled-fully-human-monoclonal-antibody-tsp-a18-reflects-transferrin-receptor-expression-in-normal-organs-and-tissues-of-mice
#6
Aya Sugyo, Atsushi B Tsuji, Hitomi Sudo, Fumiko Nomura, Hirokazu Satoh, Mitsuru Koizumi, Gene Kurosawa, Yoshikazu Kurosawa, Tsuneo Saga
Transferrin receptor (TfR) is an attractive molecule for targeted therapy of cancer. Various TfR-targeted therapeutic agents such as anti-TfR antibodies conjugated with anticancer agents have been developed. An antibody that recognizes both human and murine TfR is needed to predict the toxicity of antibody-based agents before clinical trials, there is no such antibody to date. In this study, a new fully human monoclonal antibody TSP-A18 that recognizes both human and murine TfR was developed and the correlation analysis of the radiolabeled antibody uptake and TfR expression in two murine strains was conducted...
March 2017: Oncology Reports
https://www.readbyqxmd.com/read/28166931/evaluation-of-a-panel-of-antibodies-for-the-immunohistochemical-identification-of-immune-cells-in-paraffin-embedded-lymphoid-tissues-of-new-and-old-world-camelids
#7
Ann-Kathrin Uhde, Annika Lehmbecker, Wolfgang Baumgärtner, Ingo Spitzbarth
Different species of camelids play an important role in the epidemiology of various emerging infectious diseases such as Middle East respiratory syndrome. For precise investigations of the immunopathogenesis in these host species, appropriate immunohistochemical markers are highly needed in order to phenotype distinct immune cells populations in camelids. So far, specific immunohistochemical markers for camelid immune cells are rarely commercially available, and cross-reactivity studies are restricted to the use of frozen dromedary tissues...
February 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28164137/immune-responses-to-tissue-restricted-nonmajor-histocompatibility-complex-antigens-in-allograft-rejection
#8
REVIEW
Ankit Bharat, T Mohanakumar
Chronic diseases that result in end-stage organ damage cause inflammation, which can reveal sequestered self-antigens (SAgs) in that organ and trigger autoimmunity. The thymus gland deletes self-reactive T-cells against ubiquitously expressed SAgs, while regulatory mechanisms in the periphery control immune responses to tissue-restricted SAgs. It is now established that T-cells reactive to SAgs present in certain organs (e.g., lungs, pancreas, and intestine) are incompletely eliminated, and the dysregulation of peripheral immuneregulation can generate immune responses to SAgs...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28159752/studies-of-an-androgen-binding-protein-knockout-corroborate-a-role-for-salivary-abp-in-mouse-communication
#9
Amanda G Chung, Phillip M Belone, Barbora Vošlajerová Bímová, Robert M Karn, Christina M Laukaitis
The house mouse Androgen-binding protein (Abp) gene family is comprised of 64 paralogs, 30 Abpa and 34 Abpbg, encoding the alpha (ABPA) and betagamma (ABPBG) protein subunits that are disulfide-bridged to form dimers in secretions. Only 14 Abp genes are expressed in distinct patterns in the lacrimal (11) and submandibular glands (3). We created a knockout mouse line lacking two of the three genes expressed in submandibular glands, Abpa27 and Abpbg27, by replacing them with the neomycin resistance gene. The knockout genotype (-/-) showed no Abpa27 or Abpbg27 transcripts in submandibular gland cDNA libraries and there was a concomitant lack of protein expression of ABPA27 and ABPBG27 in the -/- genotype saliva, shown by elimination of these two proteins from the saliva proteome and the loss of cross-reactive material in the acinar cells of the submandibular glands...
February 3, 2017: Genetics
https://www.readbyqxmd.com/read/28137307/dysglycemia-associations-with-adipose-tissue-among-hiv-infected-patients-after-2-years-of-antiretroviral-therapy-in-mwanza-a-follow-up-cross-sectional-study
#10
George PrayGod, John Changalucha, Saidi Kapiga, Robert Peck, Jim Todd, Suzanne Filteau
BACKGROUND: Data on the burden of dysglycemia among HIV-infected patients on antiretroviral therapy (ART) in Africa are limited. We determined the prevalence of pre-diabetes and diabetes among HIV-infected patients who started ART when malnourished 2 to 3 years previously and investigated the association of dysglycemia with body composition. METHODS: Malnourished (body mass index (BMI) < 18.5 kg/m(2)) HIV-infected patients who were enrolled in the Nutritional Support for Africans Starting Antiretroviral Therapy (NUSTART) trial from 2011 to 2013 were followed-up from March to August 2015...
January 30, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28122514/indoxyl-sulfate-the-uremic-toxin-linking-hemostatic-system-disturbances-with-the-prevalence-of-cardiovascular-disease-in-patients-with-chronic-kidney-disease
#11
Tomasz W Kamiński, Krystyna Pawlak, Małgorzata Karbowska, Michał Myśliwiec, Dariusz Pawlak
BACKGROUND: During chronic kidney disease progression, kidney-specific risk factors for cardiovascular disease come into play. The present study investigated the impact of indoxyl sulfate, dietary tryptophan-derived uremic toxin, accumulated in the blood of patients with chronic kidney disease on hemostatic parameters, markers of inflammation, oxidative stress and monocyte to macrophage transition. METHODS: Fifty-one CKD patients not undergoing hemodialysis were enrolled in the study...
January 25, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28117510/allergenicity-of-bony-and-cartilaginous-fish-molecular-and-immunological-properties
#12
REVIEW
J N Stephen, M F Sharp, T Ruethers, A Taki, D E Campbell, A L Lopata
Allergy to bony fish is common and probably increasing world-wide. The major heat-stable pan-fish allergen, parvalbumin (PV), has been identified and characterized for numerous fish species. In contrast, there are very few reports of allergic reactions to cartilaginous fish despite widespread consumption. The molecular basis for this seemingly low clinical cross-reactivity between these two fish groups has not been elucidated. PV consists of two distinct protein lineages, α and β. The α-lineage of this protein is predominant in muscle tissue of cartilaginous fish (Chondrichthyes), while β-PV is abundant in muscle tissue of bony fish (Osteichthyes)...
January 24, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28092067/interrelations-among-the-adipocytokines-leptin-and-adiponectin-oxidative-stress-and-aseptic-inflammation-markers-in-pre-and-early-pubertal-normal-weight-and-obese-boys
#13
George Paltoglou, Maria Schoina, George Valsamakis, Nicolaos Salakos, Alexandra Avloniti, Athanasios Chatzinikolaou, Alexandra Margeli, Chrysanthi Skevaki, Maria Papagianni, Christina Kanaka-Gantenbein, Ioannis Papassotiriou, George P Chrousos, Ioannis G Fatouros, George Mastorakos
PURPOSE: Presumed interrelationships among deleterious aspects of adipose tissue metabolism, inflammation, and cellular oxidative stress could be influenced by pubertal hormonal changes. They were investigated in pre- and early pubertal normal-weight and obese boys before and after an exercise bout employed as an energy demanding stimulator. METHODS: Cross-sectional study. Seventy-six healthy pre- (mean ± SD, 10.6 ± 0.2 years old, 28 normal-weight, and 11 obese) and early-(11...
January 16, 2017: Endocrine
https://www.readbyqxmd.com/read/28077404/high-throughput-sequencing-of-the-t-cell-receptor-repertoire-pitfalls-and-opportunities
#14
James M Heather, Mazlina Ismail, Theres Oakes, Benny Chain
T-cell specificity is determined by the T-cell receptor, a heterodimeric protein coded for by an extremely diverse set of genes produced by imprecise somatic gene recombination. Massively parallel high-throughput sequencing allows millions of different T-cell receptor genes to be characterized from a single sample of blood or tissue. However, the extraordinary heterogeneity of the immune repertoire poses significant challenges for subsequent analysis of the data. We outline the major steps in processing of repertoire data, considering low-level processing of raw sequence files and high-level algorithms, which seek to extract biological or pathological information...
January 10, 2017: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/28069495/surface-modification-of-copolymerized-films-from-three-armed-biodegradable-macromers-an-analytical-platform-for-modified-tissue-engineering-scaffolds
#15
Benno M Müller, Rudi Loth, Peter-Georg Hoffmeister, Friederike Zühl, Liv Kalbitzer, Michael C Hacker, Michaela Schulz-Siegmund
: The concept of macromers allows for a broad adjustment of biomaterial properties by macromer chemistry or copolymerization. Copolymerization strategies can also be used to introduce reactive sites for subsequent surface modification. Control over surface features enables adjustment of cellular reactions with regard to site and object of implantation. We designed macromer-derived polymer films which function as non-implantable analytical substrates for the investigation of surface properties of equally composed scaffolds for bone tissue engineering...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28066011/role-of-membrane-cholesterol-and-lipid-peroxidation-in-regulating-the-na-k-atpase-activity-in-schizophrenia
#16
Suparna Roy, Anindya Dasgupta, Ushasi Banerjee, Piali Chowdhury, Ashis Mukhopadhyay, Gautam Saha, Omprakash Singh
BACKGROUND: Na(+)/K(+)-ATPase (NKA) activity is compromised in several neuropsychiatric disorders. Oxidative stress and membrane lipid composition play important roles in regulating NKA activity. AIMS: The present study was undertaken to evaluate the effects of oxidative stress-induced membrane lipid damage and membrane cholesterol composition on NKA pump activity in schizophrenia. SETTINGS AND DESIGN: It was a hospital-based, cross-sectional, observational study in 49 cases and 51 controls for 1 year...
July 2016: Indian Journal of Psychiatry
https://www.readbyqxmd.com/read/28064210/characteristics-of-autoantibodies-targeting-14-3-3-proteins-and-their-association-with-clinical-features-in-newly-diagnosed-giant-cell-arteritis
#17
Anne Kistner, Marc B Bigler, Kathrin Glatz, Simon B Egli, Fabian S Baldin, Florian A Marquardsen, Matthias Mehling, Katharina M Rentsch, Daniel Staub, Markus Aschwanden, Mike Recher, Thomas Daikeler, Christoph T Berger
OBJECTIVES: Autoantibodies are useful biomarkers for diagnosing and monitoring treatment in some autoimmune diseases. Antibodies against isoforms of 14-3-3 protein have been proposed as biomarkers for the presence of aortic aneurysm in large-vessel vasculitis (LVV). Here, we aimed to evaluate the diagnostic role and potential immunopathological involvement of anti-14-3-3 antibodies in newly diagnosed LVV patients. METHODS: Antibodies against three isoforms of 14-3-3 (γ, ε and ζ) were measured in 90 subjects: 48 GCA and 3 Takayasu's arteritis (TA) patients, and 39 controls (non-inflammatory and inflammatory diseases), using a multiplexed bead-based immunoassay and immunoprecipitation studies...
January 7, 2017: Rheumatology
https://www.readbyqxmd.com/read/28063989/deep-penetrating-photodynamic-therapy-with-killerred-mediated-by-upconversion-nanoparticles
#18
Liuen Liang, Yiqing Lu, Run Zhang, Andrew Care, Tiago A Ortega, Sergey M Deyev, Yi Qian, Andrei V Zvyagin
: The fluorescent protein KillerRed, a new type of biological photosensitizer, is considered as a promising substitute for current synthetic photosensitizes used in photodynamic therapy (PDT). However, broad application of this photosensitiser in treating deep-seated lesions is challenging due to the limited tissue penetration of the excitation light with the wavelength falling in the visible spectral range. To overcome this challenge, we employ upconversion nanoparticles (UCNPs) that are able to convert deep-penetrating near infrared (NIR) light to green light to excite KillerRed locally, followed by the generation of reactive oxygen species (ROS) to kill tumour cells under centimetre-thick tissue...
January 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28058223/acute-antibody-mediated-rejection-after-intestinal-transplantation
#19
Guo-Sheng Wu, Ruy J Cruz, Jun-Chao Cai
AIM: To investigate the incidence, risk factors and clinical outcomes of acute antibody-mediated rejection (ABMR) after intestinal transplantation (ITx). METHODS: A retrospective single-center analysis was performed to identify cases of acute ABMR after ITx, based on the presence of donor-specific antibody (DSA), acute tissue damage, C4d deposition, and allograft dysfunction. RESULTS: Acute ABMR was identified in 18 (10.3%) out of 175 intestinal allografts with an average occurrence of 10 d (range, 4-162) after ITx...
December 24, 2016: World Journal of Transplantation
https://www.readbyqxmd.com/read/28046005/field-usable-lateral-flow-immunoassay-for-the-rapid-detection-of-white-spot-syndrome-virus-wssv
#20
Prabir Kumar Kulabhusan, Jyutika M Rajwade, Vimal Sugumar, Gani Taju, A S Sahul Hameed, Kishore M Paknikar
BACKGROUND: White spot disease (WSD), a major threat to sustainable aquaculture worldwide, is caused by White spot syndrome virus (WSSV). The diagnosis of WSD relies heavily on molecular detection of the virus by one-step PCR. These procedures are neither field-usable nor rapid enough considering the speed at which the virus spreads. Thus, development of a rapid, reliable and field-usable diagnostic method for the detection of WSSV infection is imperative to prevent huge economic losses...
2017: PloS One
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