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https://www.readbyqxmd.com/read/28932628/a-novel-in-silico-framework-to-improve-mhc-i-epitopes-and-break-the-tolerance-to-melanoma
#1
Cristian Capasso, Aniket Magarkar, Victor Cervera-Carascon, Manlio Fusciello, Sara Feola, Martin Muller, Mariangela Garofalo, Lukasz Kuryk, Siri Tähtinen, Lucio Pastore, Alex Bunker, Vincenzo Cerullo
Tolerance toward tumor antigens, which are shared by normal tissues, have often limited the efficacy of cancer vaccines. However, wild type epitopes can be tweaked to activate cross-reactive T-cell clones, resulting in antitumor activity. The design of these analogs (i.e., heteroclitic peptides) can be difficult and time-consuming since no automated in silico tools are available. Hereby we describe the development of an in silico framework to improve the selection of heteroclitic peptides. The Epitope Discovery and Improvement System (EDIS) was first validated by studying the model antigen SIINFEKL...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28931072/high-prevalence-of-non-dipping-blood-pressure-and-vascular-stiffness-in-hiv-infected-south-africans-on-antiretrovirals
#2
M S Borkum, J M Heckmann, K Manning, J A Dave, N S Levitt, B L Rayner, N Wearne
BACKGROUND: HIV-infected individuals are at increased risk of tissue inflammation and accelerated vascular aging ('inflamm-aging'). Abnormal diurnal blood pressure (BP) rhythms such as non-dipping may contribute to an increased risk of cardiovascular and cerebrovascular events in HIV infected individuals. However, little data exists on ambulatory blood pressure (ABP) and measures of vascular stiffness in the black African HIV infected population. METHODS: This is a cross-sectional analysis of otherwise well, HIV infected outpatients on ART for >5 years...
2017: PloS One
https://www.readbyqxmd.com/read/28930445/thermo-responsive-and-active-functional-fiber-mats-for-cultured-cell-recovery
#3
Wilaiporn Graisuwan, Songchan Puthong, Hui Zhao, Suda Kiatkamjornwong, Patrick Theato, Voravee P Hoven
Thermo-responsive and active functional fiber mats were prepared from random copolymer of poly(pentafluorophenyl acrylate-co-N-isopropylacrylamide) (P(PFPA-co-NIPAM)), which was synthesized by a controlled radical polymerization process based on reversible addition-fragmentation chain transfer (RAFT). As reactive sites, pentafluorophenyl ester (PFP) groups were incorporated in the copolymer to allow for a multiple post-polymerization modification. UV-crosslinkable moieties were first introduced by partially reacting PFP groups in the copolymer with ortho-nitrobenzyl (ONB)-protected diamine...
September 20, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28928086/mass-spectrometry-based-metabolic-signatures-of-sex-steroids-in-breast-cancer
#4
REVIEW
Man Ho Choi
Owing to controversy over the effects of steroids on breast cancer pathophysiology, comprehensive quantification of steroid hormones has been extensively considered in both clinical practice and biomarker discovery studies. In contrast to the traditional immunoaffinity-based assays, which show cross-reactivity and have poor validity at low levels of sex steroids, mass spectrometry is becoming a promising tool for measuring steroid levels in complex biological specimens. The Endocrine Society has announced and continuously updated on technical advances to apply high-quality breakthroughs in the clinical sciences...
September 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28906588/surface-glycan-pattern-of-canine-equine-and-ovine-bone-marrow-derived-mesenchymal-stem-cells
#5
Salvatore Desantis, Gianluca Accogli, Antonio Crovace, Edda G Francioso, Alberto Maria Crovace
The use of bone marrow-derived mesenchymal stem cells (MSCs) for clinical and experimental studies is increasing, but full characterization of MSCs in veterinary species is hindered by the variability in species-specific cell surface marker expression and antibody cross reactivity. Recent studies demonstrated that the glycans in the glycocalyx of MSCs are promising candidates as cell biomarkers. In the present study, we analyzed the glycocalyx of canine MSCs (cMSCs), ovine MSCs (oMSCs), and equine MSCs (eMSCs) using a cell microarray procedure in which MSCs were spotted on microarray slides and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin...
September 14, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28905812/carbon-fiber-on-polyimide-ultra-microelectrodes
#6
Wintrop F Gillis, Charles A Lissandrello, Jun Shen, Ben W Pearre, Alket Mertiri, Felix Deku, Stuart Cogan, Bradley J Holinski, Daniel J Chew, Alice E White, Timothy M Otchy, Timothy Gardner
OBJECTIVE: Most preparations for making neural recordings degrade over time and eventually fail due to insertion trauma and reactive tissue response. The magnitudes of these responses are thought to be related to the electrode size (specifically, the cross-sectional area), the relative stiffness of the electrode, and the degree of tissue tolerance for the material. Flexible carbon fiber ultra-microelectrodes have a much smaller cross-section than traditional electrodes and low tissue reactivity, and thus may enable improved longevity of neural recordings in the central and peripheral nervous systems...
September 14, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28894753/evaluation-of-epigallocatechin-3-gallate-modified-collagen-membrane-and-concerns-on-schwann-cells
#7
Chenyu Chu, Jia Deng, Cong Cao, Yi Man, Yili Qu
Collagen is an essential component of the extracellular matrix (ECM) and is a suitable material for nerve repair during tissue remodeling for fracture repair. Epigallocatechin-3-gallate (EGCG), an extract of green tea, shows various biological activities that are beneficial to nerve repair. Here, we developed modified collagen containing different concentrations of EGCG (0.0064%, 0.064%, and 0.64%, resp.) to induce Schwann cell proliferation and differentiation. Cell Counting Kit-8 test, live/dead assay, and SEM showed that collagen cross-linked by EGCG induced Schwann cell proliferation...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28890882/neutrophils-to-the-roscue-mechanisms-of-nadph-oxidase-activation-and-bacterial-resistance
#8
REVIEW
Giang T Nguyen, Erin R Green, Joan Mecsas
Reactive oxygen species (ROS) generated by NADPH oxidase play an important role in antimicrobial host defense and inflammation. Their deficiency in humans results in recurrent and severe bacterial infections, while their unregulated release leads to pathology from excessive inflammation. The release of high concentrations of ROS aids in clearance of invading bacteria. Localization of ROS release to phagosomes containing pathogens limits tissue damage. Host immune cells, like neutrophils, also known as PMNs, will release large amounts of ROS at the site of infection following the activation of surface receptors...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28887256/remote-ischemic-preconditioning-decreases-the-magnitude-of-hepatic-ischemia-reperfusion-injury-on-a-swine-model-of-supraceliac-aortic-cross-clamping
#9
G Martikos, A Kapelouzou, M Peroulis, A Paspala, D Athanasiadis, A Maheras, T D Liakakos, K Moulakakis, S Vasdekis, A M Lazaris
BACKGROUND: Temporary hepatic ischemia is inevitable during open aortic surgery when supraceliac clamping is necessary, as in thoracoabdominal or pararenal aneurysms. Remote ischemic preconditioning (RIPC) has been described as a potential protective means against ischemia - reperfusion injury (IRI) in various tissues including the liver. The aim of this experimental study was to detect the effect of RIPC on liver IRI in a model of supraceliac aortic cross-clamping. METHODS: An animal study was performed...
September 5, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28886198/tat-mediated-transduction-of-bacterial-redox-proteins-generates-a-cytoprotective-effect-on-neuronal-cells
#10
Cecilia L Balaban, Claudia Banchio, Eduardo A Ceccarelli
Cell penetrating peptides, also known as protein transduction domains, have the capacity to ubiquitously cross cellular membranes carrying many different cargos with negligible cytotoxicity. As a result, they have emerged as a powerful tool for macromolecular delivery-based therapies. In this study, catalytically active bacterial Ferredoxin-NADP+ reductase (LepFNR) and Heme oxygenase (LepHO) fused to the HIV TAT-derived protein transduction peptide (TAT) were efficiently transduced to neuroblastoma SHSY-5Y cells...
2017: PloS One
https://www.readbyqxmd.com/read/28881079/formyl-peptide-receptors-in-mice-and-men-similarities-and-differences-in-recognition-of-conventional-ligands-and-modulating-lipopeptides
#11
Malene Winther, Claes Dahlgren, Huamei Forsman
The pattern recognition formyl peptide receptors (FPRs) belong to the class of G protein-coupled receptors (GPCRs), the largest group of cell-surface receptors involved in a range of physiological processes and pathologies. The FPRs have regulatory function in the initiation as well as resolution of inflammatory reactions, making them highly interesting as targets for drug development. Recent research in the GPCR/FPR fields has uncovered novel receptor biology concepts, including biased signalling/functional selectivity, allosteric modulation, receptor reactivation and receptor cross-talk...
September 7, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28875571/cross-linked-basement-membrane-based-coatings-enhance-glucose-sensor-function-and-continuous-glucose-monitoring-in-vivo
#12
Ulrike Klueh, Izabela Ludzinska, Caroline Czajkowski, Yi Qiao, Donald L Kreutzer
Overcoming sensor induced tissue reactions is an essential element of achieving successful continuous glucose monitoring (CGM) in the management of diabetes, particularly when used in closed loop technology. Recently, we demonstrated that basement membrane (BM) based glucose sensor coatings significantly reduced tissue reactions at sites of device implantation. However, the biocompatible BM based bio-hydrogel sensor coating rapidly degraded over a less than a three-week period, which effectively eliminated the protective sensor coating...
September 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28873228/photodegradation-of-eumelanin-and-pheomelanin-and-its-pathophysiological-implications
#13
Shosuke Ito, Kazumasa Wakamatsu, Tadeusz Sarna
Eumelanin is photoprotective for pigmented tissues while pheomelanin is phototoxic. In this review, we summarize current understanding of how eumelanin and pheomelanin structures are modified by ultraviolet A (UVA) and also by visible light and how reactive oxygen species participate in those processes. Alkaline hydrogen peroxide oxidation was employed to characterize eumelanin and benzothiazole-type pheomelanin, giving pyrrole-2,3,5-tricarboxylic acid (PTCA) and thiazole-2,4,5-tricarboxylic acid (TTCA), respectively...
September 5, 2017: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/28872843/diffusion-controllable-biomineralization-conducted-in-situ-in-hydrogels-based-on-reversibly-cross-linked-hyperbranched-polyglycidol
#14
Mateusz Gosecki, Slawomir Kazmierski, Monika Gosecka
We present biocompatible hydrogel systems suitable for biomineralization processes based on hyperbranched polyglycidol cross-linked with acrylamide copolymer bearing carbonyl-coordinated boronic acid. At neutral pH, diol functional groups of HbPGL react with boronic acid of polyacrylamide to generate 3D network in water by the formation of boronic ester cross-links. The dynamic associative/dissociative characteristics of the cross-links makes the network reversible. The presented hydrogels display self-healing properties and are injectable, facilitating gap filing of bone tissue...
September 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28868796/ige-auto-reactivity-in-bullous-pemphigoid-eosinophils-and-mast-cells-as-major-targets-of-pathogenic-immune-reactants
#15
P C Freire, C H Muñoz, G Stingl
BACKGROUND: Bullous pemphigoid (BP) is an autoimmune disease characterised by tense blisters that are usually preceded by urticarial eruptions. Affected patients exhibit IgG and/or IgE auto-antibodies against BP180 and/or BP230. Their relative importance in disease pathogenesis has not been fully elucidated. OBJECTIVES: The aim of this study was to better characterise circulating and tissue-resident IgE in BP patients at the serological, structural and functional level...
September 4, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28845698/novel-cd123-aptamer-originated-targeted-drug-trains-for-selectively-delivering-cytotoxic-agent-to-tumor-cells-in-acute-myeloid-leukemia-theranostics
#16
Haibin Wu, Meng Wang, Bo Dai, Yanmin Zhang, Ying Yang, Qiao Li, Mingyue Duan, Xi Zhang, Xiaomei Wang, Anmao Li, Liyu Zhang
Since conventional chemotherapy for acute myeloid leukemia (AML) has its limitations, a theranostic platform with targeted and efficient drug transport is in demand. In this study, we developed the first CD123 (AML tumor marker) aptamers and designed a novel CD123-aptamer-mediated targeted drug train (TDT) with effective, economical, biocompatible and high drug-loading capacity. These two CD123 aptamers (termed as ZW25 and CY30, respectively) can bind to a CD123 peptide epitope and CD123 + AML cells with high specificities and KD of 29...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28834421/association-between-peripheral-adipokines-and-inflammation-markers-a-systematic-review-and-meta-analysis
#17
Sophie Graßmann, Jan Wirsching, Fabian Eichelmann, Krasimira Aleksandrova
OBJECTIVE: Obesity-induced inflammation potentially promotes a variety of chronic conditions. This study aimed to summarize cross-sectional associations between adipose tissue-derived hormones (leptin and adiponectin) and inflammatory biomarkers (C-reactive protein [CRP], interleukin [IL]-6, and tumor necrosis factor [TNF]-α) by means of meta-analysis. METHODS: A systematic search of the databases EMBASE and MEDLINE (PubMed) up to January 2017 was conducted. Data were independently extracted and evaluated by two reviewers...
August 20, 2017: Obesity
https://www.readbyqxmd.com/read/28818076/extensive-protein-hydrolyzation-is-indispensable-to-prevent-ige-mediated-poultry-allergen-recognition-in-dogs-and-cats
#18
Thierry Olivry, Jennifer Bexley, Isabelle Mougeot
BACKGROUND: The central premise for the commercialization of diets with hydrolyzed ingredients is that the small-sized digested peptides would be unable to crosslink allergen-specific IgE at the surface of tissue mast cells and induce their degranulation. Evidence for the validity of this concept to diagnose food allergies in dogs and cats is limited, however. Our objectives were to study the recognition of standard and variably hydrolyzed poultry extracts by sera from dogs and cats with elevated chicken-specific serum IgE...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28817706/hiv-infection-antiretroviral-therapy-and-measures-of-endothelial-function-inflammation-metabolism-and-oxidative-stress
#19
Andrew Dysangco, Ziyue Liu, James H Stein, Michael P Dubé, Samir K Gupta
BACKGROUND: HIV-infected patients have an increased risk of cardiovascular disease (CVD). Impaired endothelial function is an early risk factor for CVD in the general population. It is presumed that HIV infection is associated with impaired endothelial function, but results have been inconsistent. OBJECTIVES: Our objectives were to determine the relationships between HIV infection, virologic suppression with antiretroviral therapy (ART), in vivo measures of conduit artery and microvascular endothelial function, and circulating biomarkers of pathways associated with CVD...
2017: PloS One
https://www.readbyqxmd.com/read/28800601/sequence-homology-between-hla-bound-cytomegalovirus-and-human-peptides-a-potential-trigger-for-alloreactivity
#20
Charles E Hall, Vishal N Koparde, Maximilian Jameson-Lee, Abdelrhman G Elnasseh, Allison F Scalora, David J Kobulnicky, Myrna G Serrano, Catherine H Roberts, Gregory A Buck, Michael C Neale, Daniel E Nixon, Amir A Toor
Human cytomegalovirus (hCMV) reactivation may often coincide with the development of graft-versus-host-disease (GVHD) in stem cell transplantation (SCT). Seventy seven SCT donor-recipient pairs (DRP) (HLA matched unrelated donor (MUD), n = 50; matched related donor (MRD), n = 27) underwent whole exome sequencing to identify single nucleotide polymorphisms (SNPs) generating alloreactive peptide libraries for each DRP (9-mer peptide-HLA complexes); Human CMV CROSS (Cross-Reactive Open Source Sequence) database was compiled from NCBI; HLA class I binding affinity for each DRPs HLA was calculated by NetMHCpan 2...
2017: PloS One
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