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Podoplanin

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https://www.readbyqxmd.com/read/28434015/low-lymphatic-vessel-density-associates-with-chronic-rhinosinusitis-with-nasal-polyps
#1
A Luukkainen, M Seppälä, J Renkonen, R Renkonen, J Hagström, H Huhtala, M Rautiainen, J Myller, T Paavonen, A Ranta, T Torkkeli, S Toppila-Salmi
OBJECTIVES: Chronic rhinosinusitis with and without nasal polyps (CRSwNP and CRSsNP) and antrochoanal polyps (ACP) are different upper airway inflammation phenotypes with different pathomechanisms. In order to understand the development of tissue edema, the present study aimed to evaluate lymphatic vessel density in CRSsNP, CRSwNP and ACP. MATERIALS AND METHODS: 120 retrospective nasal and maxillary sinus specimens were stained immunohistochemically with a von Willebrand factor polyclonal antibody recognizing vascular and lymphatic endothelium, and with a podoplanin monoclonal antibody recognizing lymphatic endothelium...
April 23, 2017: Rhinology
https://www.readbyqxmd.com/read/28416768/tumor-hypoxia-modulates-podoplanin-ccl21-interactions-in-ccr7-nk-cell-recruitment-and-ccr7-tumor-cell-mobilization
#2
Anna Tejchman, Nathalie Lamerant-Fayel, Jean-Claude Jacquinet, Aleksandra Bielawska-Pohl, Katarzyna Mleczko-Sanecka, Catherine Grillon, Salem Chouaib, Maciej Ugorski, Claudine Kieda
Podoplanin (PDPN), an O-glycosylated, transmembrane, mucin-type glycoprotein, is expressed by cancer associated fibroblasts (CAFs). In malignant transformation, PDPN is subjected to changes and its role is yet to be established. Here we show that it is involved in modulating the activity of the CCL21/CCR7 chemokine/receptor axis in a hypoxia-dependent manner. In the present model, breast cancer MDA-MB-231 cells and NKL3 cells express the surface CCR7 receptor for CCL21 chemokine which is a potent chemoattractant able to bind to PDPN...
March 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28387612/development-of-mpmab-1-a-mouse-rat-chimeric-antibody-against-mouse-podoplanin
#3
Shinji Yamada, Mika K Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato
Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb...
April 7, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28387591/lpmab-23-a-cancer-specific-monoclonal-antibody-against-human-podoplanin
#4
Shinji Yamada, Satoshi Ogasawara, Mika K Kaneko, Yukinari Kato
Human podoplanin (hPDPN), the ligand of C-type lectin-like receptor-2, is involved in cancer metastasis. Until now, many monoclonal antibodies (mAbs) have been established against hPDPN. However, it is still difficult to develop a cancer-specific mAb (CasMab) against hPDPN because the protein sequence of hPDPN expressed in cancer cells is the same as that in normal cells. Herein, we report LpMab-23 of the mouse IgG1 subclass, a novel CasMab against hPDPN. In an immunohistochemical analysis, LpMab-23 reacted with tumor cells of human oral cancer, but did not react with normal cells such as lymphatic endothelial cells (LECs)...
April 7, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28386369/prognostic-role-of-lymphatic-vessel-density-and-lymphovascular-invasion-in-chemotherapy-naive-and-chemotherapy-treated-patients-with-invasive-breast-cancer
#5
Joanna A Niemiec, Agnieszka Adamczyk, Aleksandra Ambicka, Anna Mucha-Małecka, Wojciech M Wysocki, Beata Biesaga, Marek Ziobro, Ida Cedrych, Aleksandra Grela-Wojewoda, Małgorzata Domagała-Haduch, Joanna Wysocka, Janusz Ryś, Beata Sas-Korczyńska
It is assumed that the spread of breast cancer cells via the lymphatic system might be influenced by inflammatory reactions and/or the application of chemotherapy or molecularly targeted therapy. Therefore, we analysed survival according to lymphatic vessel density (LVD), lymphovascular invasion (LVI) (both assessed using podoplanin as immunohistochemical marker of lymphatic endothelium) and well-established clinico-pathological features in a group of 358 patients with invasive ductal breast cancer: 139 chemotherapy-naïve (pT1-2/pN0/M0) and 219 treated with chemotherapy (pT1-4/pN1-3/M0)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28385772/clec-2-podoplanin-and-thromboinflammation
#6
Katsue Suzuki-Inoue
No abstract text is available yet for this article.
April 6, 2017: Blood
https://www.readbyqxmd.com/read/28381343/podoplanin-expression-in-peritumoral-keratinocytes-predicts-aggressive-behavior-in-extramammary-paget-s-disease
#7
Zaigen Cho, Eiichi Konishi, Mai Kanemaru, Taro Isohisa, Takahiro Arita, Minako Kawai, Miho Tsutsumi, Hiromi Mizutani, Hideya Takenaka, Toshiyuki Ozawa, Daisuke Tsuruta, Norito Katoh, Jun Asai
BACKGROUND: Recent studies have demonstrated podoplanin expression in several tumors, which has been associated with lymph node metastasis and poor prognosis. Podoplanin expression in peritumoral cells such as cancer-associated fibroblasts also correlates with tumor progression in several cancers. However, podoplanin expression and its association with extramammary Paget's disease (EMPD) remain unclear. OBJECTIVE: In this study, we examined whether the presence of podoplanin expression in tumor cells or peritumoral basal keratinocytes correlated with aggressive behavior in patients with EMPD and investigated the mechanisms of podoplanin-mediated tumor invasion in this disorder...
March 27, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28369440/extravillous-trophoblast-invasion-of-venous-as-well-as-lymphatic-vessels-is-altered-in-idiopathic-recurrent-spontaneous-abortions
#8
Karin Windsperger, Sabine Dekan, Sophie Pils, Carsten Golletz, Victoria Kunihs, Christian Fiala, Glen Kristiansen, Martin Knöfler, Jürgen Pollheimer
STUDY QUESTION: Do extravillous trophoblasts (EVTs) invade non-arterial decidual vessels in healthy and pathological pregnancies? SUMMARY ANSWER: Our results reveal that trophoblast invasion of venous and lymphatic vessels is a frequent event during the first trimester of pregnancy and is compromised in  recurrent spontaneous abortion (RSA). In addition, the present data suggest that EVTs populate regional lymph nodes during pregnancy. WHAT IS ALREADY KNOWN: Human trophoblasts remodel and invade decidual spiral arteries...
March 28, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28360357/risking-thromboembolism-podoplanin-and-glioma
#9
Jeffrey I Zwicker
No abstract text is available yet for this article.
March 30, 2017: Blood
https://www.readbyqxmd.com/read/28348474/malignant-hepatic-epithelioid-hemangioendothelioma-with-villaret-syndrome
#10
Hanchanale Pavan, Joy Varghese, Kavya Harika, Mukul Vij, Venkataraman Jayanthi, Mohamed Rela
Malignant hepatic epithelioid hemangioendothelioma is a rare, vascular tumor of the liver with peak incidence in the middle age with a female preponderance and of unknown etiology. Majority of the tumors are asymptomatic. The gold standard for diagnosis of the tumor is liver histology showing presence of epithelioid tumor cells on a background of a hyaline stroma. Immunohistochemical positivity for CD31, CD34, Factor VIII, and Podoplanin (D2-40) is confirmatory. The treatment of choice is liver transplantation...
March 2017: Journal of Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/28348421/the-multifaceted-role-of-podoplanin-expression-in-hepatocellular-carcinoma
#11
Andreea Cioca, Amalia R Ceausu, Irina Marin, Marius Raica, Anca M Cimpean
The role of podoplanin in hepatocellular carcinoma (HCC) is not clear yet. The aim of our study was to evaluate the expression of podoplanin in HCC and to determine its role in hepatocarcinogenesis. We performed immunohistochemistry with monoclonal D2-40 antibody, on paraffin-embedded tissue sections of 72 patients diagnosed with HCC. Lymphatic vessels density (LVD) was increased in patients who had vascular invasion at the time of diagnosis (P=0.018) and in those with associated cirrhosis (P=0.006). Tumor cells showing podoplanin expression were correlated with histological grade (P=0...
February 13, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28339303/development-of-rap-tag-a-novel-tagging-system-for-protein-detection-and-purification
#12
Yuki Fujii, Mika K Kaneko, Satoshi Ogasawara, Shinji Yamada, Miyuki Yanaka, Takuro Nakamura, Noriko Saidoh, Kanae Yoshida, Ryusuke Honma, Yukinari Kato
Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification...
March 24, 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28333941/phenotypically-heterogeneous-podoplanin-expressing-cell-populations-are-associated-with-the-lymphatic-vessel-growth-and-fibrogenic-responses-in-the-acutely-and-chronically-infarcted-myocardium
#13
Maria Cimini, Antonio Cannatá, Gianandrea Pasquinelli, Marcello Rota, Polina Goichberg
Cardiac lymphatic vasculature undergoes substantial expansion in response to myocardial infarction (MI). However, there is limited information on the cellular mechanisms mediating post-MI lymphangiogenesis and accompanying fibrosis in the infarcted adult heart. Using a mouse model of permanent coronary artery ligation, we examined spatiotemporal changes in the expression of lymphendothelial and mesenchymal markers in the acutely and chronically infarcted myocardium. We found that at the time of wound granulation, a three-fold increase in the frequency of podoplanin-labeled cells occurred in the infarcted hearts compared to non-operated and sham-operated counterparts...
2017: PloS One
https://www.readbyqxmd.com/read/28332312/antitumor-activity-of-chlpmab-2-a-human-mouse-chimeric-cancer-specific-antihuman-podoplanin-antibody-via-antibody-dependent-cellular-cytotoxicity
#14
Mika K Kaneko, Shinji Yamada, Takuro Nakamura, Shinji Abe, Yasuhiko Nishioka, Akiko Kunita, Masashi Fukayama, Yuki Fujii, Satoshi Ogasawara, Yukinari Kato
Human podoplanin (hPDPN), a platelet aggregation-inducing transmembrane glycoprotein, is expressed in different types of tumors, and it binds to C-type lectin-like receptor 2 (CLEC-2). The overexpression of hPDPN is involved in invasion and metastasis. Anti-hPDPN monoclonal antibodies (mAbs) such as NZ-1 have shown antitumor and antimetastatic activities by binding to the platelet aggregation-stimulating (PLAG) domain of hPDPN. Recently, we developed a novel mouse anti-hPDPN mAb, LpMab-2, using the cancer-specific mAb (CasMab) technology...
April 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28319578/podoplanin-and-sox2-expression-in-cin-3-like-squamous-cell-carcinoma-of-the-cervix
#15
Colin J R Stewart, Maxine L Crook
Squamous cell carcinoma (SCC) of the uterine cervix occasionally demonstrates a deceptive growth pattern that mimics endocervical crypt involvement by cervical intraepithelial neoplasia, grade 3 (CIN 3). Such CIN 3-like SCCs may be misinterpreted as noninvasive or minimally invasive leading to delays in diagnosis. Little is known of the factors that influence the growth patterns of cervical SCC but we suggested recently that CIN 3-like tumors might demonstrate "collective cellular invasion," which is characterized by a retained epithelial phenotype...
March 17, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28315338/lymphatic-and-vascular-markers-in-an-optic-nerve-crush-model-in-rat
#16
A Trost, D Bruckner, A Kaser-Eichberger, K Motloch, B Bogner, C Runge, C Strohmaier, S Couillard-Despres, H A Reitsamer, F Schroedl
Only few tissues lack lymphatic supply, such as the CNS or the inner eye. However, if the scleral border is compromised due to trauma or tumor, lymphatics are detected in the eye. Since the situation in the optic nerve (ON), part of the CNS, is not clear, the aim of this study is to screen for the presence of lymphatic markers in the healthy and lesioned ON. Brown Norway rats received an unilateral optic nerve crush (ONC) with defined force, leaving the dura intact. Lesioned ONs and unlesioned contralateral controls were analyzed 7 days (n = 5) and 14 days (n = 5) after ONC, with the following markers: PDGFRb (pericyte), Iba1 (microglia), CD68 (macrophages), RECA (endothelial cell), GFAP (astrocyte) as well as LYVE-1 and podoplanin (PDPN; lymphatic markers)...
March 14, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28295004/-serrated-adenomas-of-the-colon-clinical-morphological-and-molecular-genetic-characteristics
#17
L M Mikhaleva, R A Komleva, A E Biryukov, N K Shakhpazyan
AIM: to investigate the clinical, morphological, and molecular genetic characteristics of serrated adenomas of the colon. MATERIAL AND METHODS: The study included 82 colon adenomas from 63 patients aged 29 to 81 years, who underwent colonoscopy with biopsy or polypectomy. The mean age of men was 63.3 years; that of women was 56.8 years. Comprehensive clinical, morphological, immunohistochemical, and molecular genetic examinations were made. RESULTS: The histological examination showed that sessile serrated adenomas (SSA) of the colon were most common (46...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/28294559/the-platelet-activating-receptor-c-type-lectin-receptor-2-plays-an-essential-role-in-liver-regeneration-after-partial-hepatectomy-in-mice
#18
H Kono, H Fujii, K Suzuki-Inoue, O Inoue, S Furuya, K Hirayama, Y Akazawa, Y Nakata, C Sun, N Tsukiji, T Shirai, Y Ozaki
Essentials Regeneration role of C-type lectin receptor-2 (CLEC-2) after 70% hepatectomy (HPx) was investigated. Wild-type or CLEC-2 deleted from platelets of chimeric mice (flKO) underwent HPx. The liver/body weight ratio was significantly lower in the flKO than in the wild-type. CLEC-2 plays an essential role in liver regeneration after HPx. SUMMARY: Background and aim The aim of the present study was to investigate the role of C-type lectin receptor (CLEC)-2 in liver regeneration following partial liver resection in mice...
March 12, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28262465/characterization-of-human-fibroblastic-reticular-cells-as-potential-immunotherapeutic-tools
#19
Jaris Valencia, Eva Jiménez, Víctor G Martínez, Beatriz G Del Amo, Laura Hidalgo, Ana Entrena, Lidia M Fernández-Sevilla, Francisco Del Río, Alberto Varas, Ángeles Vicente, Rosa Sacedón
Fibroblastic reticular cells (FRCs) are essential players during adaptive immune responses not only as a structural support for the encounter of antigen-presenting cells and naive T lymphocytes but also as a source of modulatory signals. However, little is known about this cell population in humans. To address the phenotypical and functional analysis of human FRCs here we established splenic (SP) and mesenteric lymph node (LN) CD45(-)CD31(-)CD90(+)podoplanin(+) myofibroblastic cell cultures. They shared the phenotypical characteristics distinctive of FRCs, including the expression of immunomodulatory factors and peripheral tissue antigens...
May 2017: Cytotherapy
https://www.readbyqxmd.com/read/28261205/human-lymph-node-derived-fibroblastic-and-double-negative-reticular-cells-alter-their-chemokines-and-cytokines-expression-profile-following-inflammatory-stimuli
#20
Patricia Severino, Diana Torres Palomino, Heliene Alvarenga, Camila Bononi Almeida, Denise Cunha Pasqualim, Adriano Cury, Paolo Rogério Salvalaggio, Antonio Luiz De Vasconcelos Macedo, Maria Claudina Andrade, Thiago Aloia, Silvio Bromberg, Luiz Vicente Rizzo, Fernanda Agostini Rocha, Luciana C Marti
Lymph node (LN) is a secondary lymphoid organ with highly organized and compartmentalized structure. LNs harbor B, T, and other cells among fibroblastic reticular cells (FRCs). FRCs are characterized by both podoplanin (PDPN/gp38) expression and by the lack of CD31 expression. FRCs are involved in several immune response processes but mechanisms underlying their function are still under investigation. Double-negative cells (DNCs), another cell population within LNs, are even less understood. They do not express PDPN or CD31, their localization within the LN is unknown, and their phenotype and function remain to be elucidated...
2017: Frontiers in Immunology
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