Xiaoxue Fan, Zhanying Chu, Manman Zhu, Yumeng Song, Yang Zhao, Bo Meng, Xiaoyun Gong, Di Zhang, You Jiang, Liqing Wu, Keiichi Tamiya, Xiaoping Yu, Rui Zhai, Xinhua Dai, Xiang Fang
In proteomics research, with advantages including short digestion times and reusable applications, immobilized enzyme reactors (IMERs) have been paid increasing attention. However, traditional IMERs ignore the reasonable spatial arrangement of trypsin on the supporting matrixes, resulting in the partial overlapping of the active domain on trypsin and reducing digesting efficiency. In this work, a DNA tetrahedron (DNA TET)-based IMER Fe3 O4 -GO-AuNPs-DNA TET-Trypsin was designed and prepared. The distance between vertices of DNA TETs effectively controls the distribution of trypsin on the nanomaterials; thus, highly efficient protein digestion and accurate quantitative results can be achieved...
October 18, 2023: Analytical Chemistry