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Christine Mannhalter
Today, we have access to excellent and advanced molecular methods that are already widely used. This requires rules to control the quality of the methods as well as the laboratory. Both aspects will be discussed in the article. Following the isolation of nucleic acids they are used for genotyping which allows to address several questions: diagnosis of inherited diseases, inherited predispositions, forensic analyses, identification and typing of bacteria or viruses, elucidation of evolutionary aspects. Importantly, it has to be realized that the type and heterogeneity of phenotypically relevant mutations determines the method used for testing...
May 10, 2017: Hämostaseologie
T C Häußler, C Peppler, S Schmitz, C Bauer, J Hirzmann, M Kramer
In a female dog with unspecific clinical symptoms, sonography detected a hyperechoic mass in the middle abdomen and blood analysis a middle grade systemic inflammatory reaction. Laparotomy revealed a peritoneal larval cestodosis (PLC). The diagnosis of an infection with tetrathyridia of Mesocestoides spp. was confirmed by parasitological examination and molecularbiological analysis. Reduction of the intra-abdominal parasitic load as well as a high dose administration of fenbendazole over 3 months led to a successful treatment which could be documented sonographically and by decreased concentrations of C-reactive protein (CRP)...
2016: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
Michael Mayer, Adrian Keller, Ulrich Szewzyk, Hans-Joachim Warnecke
Pure drinking water is the basis for a healthy society. In Germany the drinking water regulations demand for analysis of water via detection of certain microbiological parameters by cultivation only. However, not all prokaryotes can be detected by these standard methods. How to gain more and better information about the bacteria present in drinking water and its distribution systems? The biofilms in drinking water distribution systems are built by bacteria and therefore represent a valuable source of information about the species present...
May 10, 2015: Journal of Biotechnology
Kristie Nybo
This month's question from the Molecular Biology Forums (online at comes from the "RNA Methods" section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or the opinions of, BioTechniques.
April 2013: BioTechniques
S Schulz, N Zissler, W Altermann, J Klapproth, U Zimmermann, C Gläser, H-G Schaller, S Reichert
CD14 and toll-like receptor 4 (TLR4) are involved in host's immune response to bacterial pathogens including periodontal bacteria. Functional important gene polymorphisms are described for both genes. The aim of this study was to evaluate links between genetic polymorphisms of CD14 and TLR4 and risk markers of periodontitis in a multivariate model. One hundred and thirty-three periodontitis patients (chronic: n = 60, aggressive: n = 73) and 80 healthy controls without periodontitis were included in the study...
December 2008: International Journal of Immunogenetics
Guido Michels, Fikret Er, Ismail F Khan, Jeannette Endres-Becker, Mathias C Brandt, Natig Gassanov, David C Johns, Uta C Hoppe
Hyperpolarization-activated, cyclic nucleotide sensitive (HCN) channels underlie the pacemaker current I(f), which plays an essential role in spontaneous cardiac activity. HCN channel subunits (HCN1-4) are believed to be modulated by additional regulatory proteins, which still have to be identified. Using biochemistry, molecularbiology and electrophysiology methods we demonstrate a protein-protein interaction between HCN2 and the K(+) channel regulator protein 1, named KCR1. In coimmunoprecipitation experiments we show that KCR1 and HCN2 proteins are able to associate...
2008: PloS One
Herbert Auer, Heinrich Leskowschek, Jörg Engler, Gerhard Leitner, Christa Wentzel, Walburg Wolkerstorfer, Renate Schneider
Two cases of anisakiosis are reported. Two male patients (54 and 58 years old) had spent their holidays in Alaska for salmon fishing at the end of July 2006 and consumed the self caught cold smoked salmon. Back in Austria both patients suffered from abdominal pain within 6 and 15 days respectively, after consumption and received in-patient treatment (patient 1: subileus; patient 2: ileus). Patient 1 received aprednisolon alone and recovered within 3 days, patient 2, however, was treated surgically (ileus) and suffered from an ARDS and an insufficiency of anastomosis during postoperative intensive therapy, additionally he received hydrocortisone...
2007: Wiener Klinische Wochenschrift
C Härtel, A Herz, S Vieth, C Lensing, C Schultz
A previously healthy two-year-old girl presented with proteinuria and macroscopic haematuria. Laboratory findings included haemolytic anaemia with thrombocytopenia. Interestingly, continuing reticulocytopenia was noted. Therefore an acute parvovirus B19 infection was suspected, which could be confirmed by serological and molecularbiological evidence. This case report underlines renal complications of parvovirus B19 infection in early childhood including haemolytic-uraemic syndrome (HUS)-like episodes, and potential pathogenetic mechanisms are discussed...
March 2007: Klinische Pädiatrie
S Dreier, J L Khol, B Stein, K Fuchs, S Gütler, W Baumgartner
Paratuberculosis (Johne's disease) is a chronic infectious disease of ruminants, caused by Mycobacterium avium subspecies paratuberculosis (MAP). Because of its long incubation period, high economic losses, difficulties in diagnosis and possible links to Morbus Crohn in humans, paratuberculosis is one of the most important diseases of ruminants today. An abattoir-based nationwide survey on the occurrence of MAP in the Austrian cattle population was performed using serology (SVANOVIR-ELISA) as well as culture, ZN-stain and IS900-PCR on faeces and lymph node samples...
December 2006: Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
H Bürger, C Kersting, D Hungermann, T Decker, W Böcker
Only little information on the primary molecularbiological events involved in early breast is available. In particular, the definition of postulated precursor lesions of invasive breast cancer, such as ductal hyperplasia or ductal carcinoma in situ, is under an intense, controversial discussion in terms of pathogenesis and tumor biology. The most recent research on biological regulation mechanisms and genetic alterations in morphologically normally appearing breast tissue give rise for a reinterpretation for the most common progression models of breast cancer...
September 2006: Der Pathologe
Sebastian Fischer, Katrin Strutzberg-Minder, Gundi Müller, Matthias Homuth
Herpesvirus infections are significant in the care of turtles and tortoises. Clinical signs range from unspecific symptoms, due to the variety of organ manifestations, to the "classical" picture of rhinitis-stomatitis. The presented case study showed the typical disease only with respect to clinical symptoms following hibernation, but lacks stomatitis, erosions or plaques in the oral mucosa. On the other hand, skin lesions on the extremeties, causative with herpesvirus infection, could be diagnosed. In this case study, various symptoms, sampling procedures and diagnostics using two different PCR methods are presented...
January 2006: Berliner und Münchener Tierärztliche Wochenschrift
S Koscielny, F V Eggeling, R Dahse
BACKGROUND: The inactivation of the tumor suppressor gene p16 plays an important role in the development of malignant tumors. P16 loss can result from point mutations, loss of heterozygosity (LOH) or methylation of the promoter region. MATERIAL AND METHODS: A total of 67 samples of tumor tissue from squamous cell carcinomas of the oral cavity, the pharynx and the larynx were analysed for an inactivation of p16. The samples were obtained during surgery. In all cases there was a curative intention...
June 2004: Laryngo- Rhino- Otologie
Heinz-C Schröder, Sanja Perović-Ottstadt, Matthias Rothenberger, Matthias Wiens, Heiko Schwertner, Renato Batel, Michael Korzhev, Isabel M Müller, Werner E G Müller
Silicon is, besides oxygen, the most abundant element on earth. Only two taxa use this element as a major constituent of their skeleton, namely sponges (phylum Porifera) and unicellular diatoms. Results from combined cytobiological and molecularbiological techniques suggest that, in the demosponge Suberites domuncula, silicic acid is taken up by a transporter. Incubation of cells with the fluorescent silica tracer PDMPO [2-(4-pyridyl)-5-[[4-(2-dimethylaminoethylaminocarbamoyl)methoxy]phenyl]-oxazole] showed a response to silicic acid by an increase in fluorescence; this process is temperature-dependent and can be blocked by DIDS (4,4-di-isothiocyanatostilbene-2,2-disulphonic acid)...
August 1, 2004: Biochemical Journal
Peter Gallien
Shigatoxin-producing E. coli (STEC) and their subgroup, the enterohaemorrhagic E. coli (EHEC) are known since about 25 years. Only EHEC can cause diseases in humans. A molecularbiological method cascade is described in this article. It contains the following steps: preenrichment, enrichment, preparation of samples (DNA extraction), screening PCR, specific STEC isolation by using DNA DNA hybridization, verification of isolates as STEC by using PCR, characterization of STEC isolates. Pulsed field gel electrophoresis and DNA sequencing are additional methods for the detection of clonal correlations...
November 2003: Berliner und Münchener Tierärztliche Wochenschrift
J G Kuipers, L Köhler, H Zeidler
Microbiological diagnosis for rheumatic diseases is increasingly used as part of the diagnostic work-up in rheumatological practice due to growing knowledge about bacteria-induced rheumatic diseases. This review's focus lies on rheumatic diseases, which in contrast to septic-infectious arthritis, are characterized by the inability to culture bacteria from the inflammed joint. These reactive arthritides occur after primary extraarticular bacterial infection. The etiological diagnosis of reactive arthritis is based on the detection of a previous or ongoing bacterial infection...
August 2002: Zeitschrift Für Rheumatologie
M Seitz, P Villiger
Laboratory investigations play an important part in the diagnostic procedures of inflammatory rheumatic diseases. Besides the classical humoral inflammatory parameters (erythrocyte sedimentation rate, C-reactive protein, protein electrophoresis), hematology, blood chemistry, infectious serology and synovial fluid analysis we want to focus in this review on the more detailed diagnostic by autoantibody testing as well as by specific molecularbiological procedures (PCR technique).
January 16, 2002: Praxis
R Göder, V Treskov, J Burmester, J B Aldenhoff, D Hinze-Selch
Our group as well as about 20 other publications report cases of dependence from zolpidem. Furthermore, there is epidemiological and polysomnographic evidence that there is a risk for tolerance and dependence for zolpidem although lower than in the case of benzodiazepines. Recent molecularbiological findings offer interesting data in this respect. Whereas in the recommended dose range zolpidem almost exclusively binds to the alpha(1) subunit of the GABA(A) receptor associated with sleep promotion, in higher doses it also binds the alpha(2), alpha(3) and alpha(5) subunits typically targeted by benzodiazepines and associated with anxiolytic effects...
December 2001: Fortschritte der Neurologie-Psychiatrie
T Bächle, U Rühl, G Ott, S Walker
HISTORY AND CLINICAL FINDINGS: A 61-year-old woman in poor general health was admitted to hospital because of progressive diarrheo, flatulence, fatigue and weight loss. The patient had a history of coeliac disease with poor dietary compliance over many years. Hence, the present clinical deterioration was unresponsive to a gluten-free diet. INVESTIGATIONS: Laboratory results showed signs of inflammation and malabsorption. The endoscopy of the duodenum revealed a flattened mucosal architecture, as is typical for coeliac disease...
December 21, 2001: Deutsche Medizinische Wochenschrift
H. Rübben
The last years were characterized by efforts on the field of urological oncology: development of highly effective regimens for the curative treatment of metastatic diseases, improvement of diagnostic techniques based on monoclonal antibodies and molecularbiological techniques, development of operative techniques to improve quality of life in tumor patients. The cisplatin-based chemotherapy led to a dramatic improvement in patients with even extended testicular cancer disease and reflect one of the major highlights in urological oncology in the last century...
October 2000: Onkologie
R Weiss, P Raabe, P Mayser
The historical development of the taxonomic classification of Malassezia yeasts until today yielded the description of seven different species based upon molecularbiological, morphological and biochemical parameters (M. furfur, M. pachydermatis, M. sympodialis, M. globosa, M. obtusa, M. restricta, M. slooffiae). Tests like Cremophor EL assimilation, esculin degradation, catalase test, pigment production and determination of polidocanol sensitivity appear to be suitable procedures for routine identification of the different species...
2000: Mycoses
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