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https://www.readbyqxmd.com/read/28337426/pathogen-identification-in-suspected-cases-of-pyogenic-spondylodiscitis
#1
Ahmad Farajzadeh Sheikh, Azar D Khosravi, Hamed Goodarzi, Roohangiz Nashibi, Alireaza Teimouri, Azim Motamedfar, Reza Ranjbar, Sara Afzalzadeh, Mehrandokht Cyrus, Mohammad Hashemzadeh
Pyogenic spinal infection continues to represent a worldwide problem. In approximately one-third of patients with pyogenic spondylodiscitis, the infectious agent is never identified. Of the cases that lead to organismal identification, bacteria are more commonly isolated from the spine rather than fungi and parasites. This study applied universal prokaryotic 16S rRNA PCR as a rapid diagnostic tool for the detection of bacterial agents in specimens from patients suspected of pyogenic spondylodiscitis. Gram and Ziehl-Neelsen staining were used as a preliminary screening measure for microbiologic evaluation of patient samples...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28335406/factors-that-negatively-affect-the-prognosis-of-pediatric-community-acquired-pneumonia-in-district-hospital-in-tanzania
#2
Serena Caggiano, Nicola Ullmann, Elisa De Vitis, Marzia Trivelli, Chiara Mariani, Maria Podagrosi, Fabiana Ursitti, Chiara Bertolaso, Carolina Putotto, Marta Unolt, Andrea Pietravalle, Paola Pansa, Kajoro Mphayokulela, Maria Incoronata Lemmo, Michael Mkwambe, Joseph Kazaura, Marzia Duse, Francesco Nieddu, Chiara Azzari, Renato Cutrera
Community-acquired pneumonia (CAP) is still the most important cause of death in countries with scarce resources. All children (33 months ± 35 DS) discharged from the Pediatric Unit of Itigi Hospital, Tanzania, with a diagnosis of CAP from August 2014 to April 2015 were enrolled. Clinical data were gathered. Dried blood spot (DBS) samples for quantitative real-time polymerase chain reaction (PCR) for bacterial detection were collected in all 100 children included. Twenty-four percent of patients were identified with severe CAP and 11% died...
March 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28330484/antibiotic-resistance-and-biofilm-production-among-the-strains-of-staphylococcus-aureus-isolated-from-pus-wound-swab-samples-in-a-tertiary-care-hospital-in-nepal
#3
Ankit Belbase, Narayan Dutt Pant, Krishus Nepal, Bibhusan Neupane, Rikesh Baidhya, Reena Baidya, Binod Lekhak
BACKGROUND: The increasing drug resistance along with inducible clindamycin resistance, methicillin resistance and biofilm production among the strains of Staphylococcus aureus are present as the serious problems to the successful treatment of the infections caused by S. aureus. So, the main objectives of this study were to determine the antimicrobial susceptibility patterns along with the rates of inducible clindamycin resistance, methicillin resistance and biofilm production among the strains of S...
March 23, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28300288/trends-and-outcomes-of-infective-endocarditis-in-patients-on-dialysis
#4
Nirmanmoh Bhatia, Sahil Agrawal, Aakash Garg, Divyanshu Mohananey, Abhishek Sharma, Manyoo Agarwal, Lohit Garg, Nikhil Agrawal, Amitoj Singh, Sudip Nanda, Jamshid Shirani
Dialysis patients are at high risk for infective endocarditis (IE); however, no large contemporary data exist on this issue. We examined outcomes of 44 816 patients with IE on dialysis and 202 547 patients with IE not on dialysis from the Nationwide Inpatient Sample database from 2006 thorough 2011. Dialysis patients were younger (59 ± 15 years vs 62 ± 18 years) and more likely to be female (47% vs 40%) and African-American (47% vs 40%; all P < 0.001). Hospitalizations for IE in the dialysis group increased from 175 to 222 per 10 000 patients (P trend  = 0...
March 16, 2017: Clinical Cardiology
https://www.readbyqxmd.com/read/28297697/evaluation-of-infection-resistance-of-biodegradable-versus-conventional-annuloplasty-rings-in-an-in-vivo-rat-subcutaneous-model
#5
Ilhan Ozgol, Burak Can Depboylu, Aybala Tongut, Sinem Ozdemir, Yasar Bagdatlı, Sanaz Ainechi, Buge Oz, Jean-Claude Pache, Selim Erenturk, Afksendiyos Kalangos, Ali Can Hatemi, Mustafa Cikirikcioglu
BACKGROUND: Biodegradable atrioventricular annuloplasty rings are theoretically more infection resistant due to their intra-annular implantation technique and nonporous structures (monofilament of poly-1,4-dioxanone). The aim of this study was to investigate the infection resistance of a biodegradable annuloplasty ring (Kalangos-Bioring®) in a rat subcutaneous implantation model and to compare it with a commonly used conventional annuloplasty ring (Edwards Physio II®). METHODS: This study included 32 Wistar albino rats which were divided into 2 groups according to the implantation of sterile or infected annuloplasty rings as control and study groups...
March 16, 2017: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/28294933/differences-in-sheep-and-goats-milk-microbiological-profile-between-conventional-and-organic-farming-systems-in-greece
#6
Eleni Malissiova, Theofilos Papadopoulos, Aikaterini Kyriazi, Maria Mparda, Christina Sakorafa, Antonios Katsioulis, Anna Katsiaflaka, Maria Kyritsi, Antonios Zdragas, Christos Hadjichristodoulou
The aim of this study was to examine differences in the microbiological profile and antimicrobial resistance of bacteria isolated from milk from organic and conventional sheep and goat farms. Twenty-five organic and 25 conventional sheep and goat farms in the region of Thessaly, Greece participated in this study. A standardised detailed questionnaire was used to describe farming practices. A total of 50 samples were collected and analysed for total viable count (TVC), total coliform count (TCC) and somatic cell count (SCC), while Staphylococcus aureus and Escherichia coli were isolated using standard methods...
March 15, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28290221/microbiological-diagnosis-and-antimicrobial-sensitivity-profiles-in-diseased-free-living-raptors
#7
Anna Vidal, Laia Baldomà, Rafael A Molina-Lopez, Marga Martin, Laila Darwich
Free-living raptors (birds of prey) can act as reservoirs of potentially zoonotic agents but they also can be affected by microorganisms as target hosts. In this retrospective study, microbiological results (n=663) and antibiotic sensitivity profiles (n=108) of bacterial isolates were analyzed from diseased free-living raptors. Sixty-nine percent of cases (n=457) yielded bacteria: 58% were in pure culture and 42% were of different species. Remarkably, samples from necropsies (47%) had higher percentage of pure isolations than those obtained from clinical (31%) samples (p<0...
March 14, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28284196/burden-of-bloodstream-infection-in-an-area-of-mid-norway-2002-2013-a-prospective-population-based-observational-study
#8
Arne Mehl, Bjørn Olav Åsvold, Stian Lydersen, Julie Paulsen, Erik Solligård, Jan Kristian Damås, Stig Harthug, Tom-Harald Edna
BACKGROUND: Studies from several countries indicate that the incidence and mortality of bloodstream infection (BSI) have been increasing over time. METHODS: We studied the burden of disease and death related to BSI in a defined geographical area of Mid-Norway, where BSI episodes were prospectively recorded by the same microbiological department during 12 consecutive years. Death from BSI was defined as death within 30 days of BSI detection. Age and sex standardized incidence and mortality rates and case fatality rates were calculated...
March 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28284111/characteristics-and-predictors-of-outcome-of-spontaneous-spinal-epidural-abscesses-treated-conservatively-a-retrospective-cohort-study-in-a-referral-center
#9
Nikolaos Spernovasilis, Stelios Demetriou, Maria Bachlitzanaki, Ioannis Gialamas, Kalliopi Alpantaki, Georgios Hamilos, Apostolos Karantanas, Achilleas Gikas
OBJECTIVE: Recent studies have shown that in carefully selected patients, conservative treatment alone can be an option in the management of spinal epidural abscess (SEA). The aim of this study was to identify prognostic factors of outcome in patients with spontaneous SEA treated conservatively. PATIENTS AND METHODS: A retrospective cohort study of all patients with spontaneous SEA treated with antibiotics alone from January 2012 to December 2015 was conducted in a 1200-bed tertiary referral center...
February 27, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28271855/prevalence-of-foodborne-pathogens-in-food-from-selected-african-countries-a-meta-analysis
#10
REVIEW
Narayan Paudyal, Victor Anihouvi, Joseph Hounhouigan, Maitshwarelo Ignatius Matsheka, Bonno Sekwati-Monang, Wisdom Amoa-Awua, Amy Atter, Nina Bernice Ackah, Samuel Mbugua, Agnes Asagbra, Warda Abdelgadir, Jesca Nakavuma, Mogens Jakobsen, Weihuan Fang
Food safety information in the African region is insufficient and fragmented due to lack of surveillance, documentation and reporting, thereby resulting in inefficient utilization of resources, duplication of activities, and lack of synergy among the countries of the region. This paper reviews the prevalence of foodborne pathogens in seven African countries (Benin, Botswana, Ghana, Kenya, Nigeria, Sudan and Uganda) from papers in regional or international journals published between January 2000 and December 2015...
March 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28260753/molecular-characterization-of-methicillin-resistance-staphylococcus-aureus-mrsa-from-different-tertiary-care-hospitals-in-bangladesh
#11
S Roy, T K Barman, M A Hossain, S K Paul, N Haque, S Ahmed, S A Nasreen, M S Hossain, S R Sarkar, N Kubayashi, N Laskar
Infections caused by Staphylococcus aureus were treated by methicillin, but about 95% of S. aureus has been resistance to methicillin, both in the community and hospitals and are increasing day by day. MRSA produces altered penicillin binding protein, PBP2a, due to the expression of mecA gene. Some strains of both the MRSA and MSSA carry PVL gene. This cross sectional observational study was conducted to detect the molecular-characterization of methicillin resistant Staphylococcus aureus (MRSA) and carried out in the Department of Microbiology, Mymensingh Medical College from July 2014 to December 2015...
January 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/28244481/epidemiology-and-resistance-pattern-of-bacterial-isolates-among-cancer-patients-in-a-tertiary-care-oncology-centre-in-north-india
#12
U Batra, P Goyal, P Jain, A Upadhyay, N Sachdeva, M Agarwal, D Bhurani, V Talwar, S K Gupta, D C Doval
OBJECTIVES: To examine the epidemiology of microbiologically documented bacterial infection and the resistance pattern, among cancer patients undergoing treatment at RGCIRC, Delhi. DESIGN AND SETTING: Retrospective observational study in which culture reports obtained over 1 year in 2013, were analyzed. RESULTS: 13329 cultures were obtained over 1 year in 2013 and were analyzed. 23.6 % samples showed positive culture with majority being gram negative isolates (67...
July 2016: Indian Journal of Cancer
https://www.readbyqxmd.com/read/28236499/clinical-features-and-microbiological-in-bacterial-keratitis-in-a-tertiary-referral-hospital
#13
J M Ruiz Caro, L Cabrejas, M R de Hoz, D Mingo, S P Duran
OBJECTIVE: To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid. MATERIALS AND METHODS: A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014. RESULTS: In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64...
February 21, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/28225917/oxacillin-magnetically-targeted-for-the-treatment-of-methicillin-resistant-s-aureus-infection-in-rats
#14
Juliana Fernandes de Carvalho, Ítalo Medeiros de Azevedo, Keyla Borges Ferreira Rocha, Aldo Cunha Medeiros, Artur da Silva Carriço
Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles...
January 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/28216296/cyclodextrin-modified-plla-parietal-reinforcement-implant-with-prolonged-antibacterial-activity
#15
G Vermet, S Degoutin, F Chai, M Maton, C Flores, C Neut, P E Danjou, B Martel, N Blanchemain
The use of textile meshes in hernia repair is widespread in visceral surgery. Though, mesh infection is a complication that may prolong the patient recovery period and consequently presents an impact on public health economy. Such concern can be avoided thanks to a local and extended antibiotic release on the operative site. In recent developments, poly-L-lactic acid (PLLA) has been used in complement of polyethyleneterephthalate (Dacron(®)) (PET) or polypropylene (PP) yarns in the manufacture of semi-resorbable parietal implants...
February 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28216018/prospective-randomised-controlled-study-evaluating-early-modification-of-oral-microbiota-following-admission-to-the-intensive-care-unit-and-oral-hygiene-with-chlorhexidine
#16
Felipe Francisco Tuon, Oleg Gavrilko, Saulo de Almeida, Eigi Ricardo Sumi, Thiago Alberto, Jaime Luis Rocha, Edvaldo Antonio Rosa
OBJECTIVES: Chlorhexidine (CHX) is the most commonly used oral hygiene product for the prevention of ventilator-associated pneumonia (VAP) in patients undergoing mechanical ventilation (MV). The change in dental plaque (DP) microbiota following CHX use in patients under MV has not been described previously. The aim of this study was to evaluate the incidence of pathogenic bacteria associated with VAP and the coverage of DP within the oral cavity in patients administered CHX. METHODS: This was a prospective, randomised, controlled, double-blind study in patients (n=16) under MV who were mouth-rinsed with either CHX or placebo...
February 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28216007/microbiological-quality-of-pastrami-and-associated-surfaces-at-the-point-of-sale-in-kayseri-turkey
#17
Y Yildirim, N Ertas Onmaz, Z Gonulalan, S Al, A Yildirim, F Karadal, H Hizlisoy, Ş Pamuk
OBJECTIVE: The aim of this study is to trace the possible relations between the hygienic status of slicing utensils and the microbiological quality of pastrami. STUDY DESIGN: A total of 75 pastrami retail markets were visited in Kayseri, Turkey, where the pastrami (a ready-to-eat meat product) is commonly produced and consumed. Sliced pastrami, the cutting board and knife surface swabs were collected from each pastrami retail point to trace possible sources of contamination...
February 16, 2017: Public Health
https://www.readbyqxmd.com/read/28215714/microbiological-effect-of-mupirocin-and-chlorhexidine-for-staphylococcus-aureus-decolonization-in-community-and-nursing-home-based-adults
#18
Mary-Claire Roghmann, Alison D Lydecker, Patricia Langenberg, Emmanuel F Mongodin, J Kristie Johnson
OBJECTIVE: To compare the presence of Staphylococcus aureus and pathogenic Gram-negative rods (GNR) in the anterior nares, posterior pharynx and three skin sites in community-based adults and nursing home-based adults before and after treatment with nasal mupirocin and topical chlorhexidine. METHODS: S. aureus-colonized adults were recruited from the community (n=26) and from nursing homes (n=8). Eligible participants were cultured for S. aureus and GNR during two study visits and then received intranasal mupirocin and topical chlorhexidine for 5days, with a 2-month follow-up period...
February 13, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28214695/the-effects-of-atmospheric-pressure-cold-plasma-treatment-on-microbiological-physical-chemical-and-sensory-characteristics-of-vacuum-packaged-beef-loin
#19
A Bauer, Y Ni, S Bauer, P Paulsen, M Modic, J L Walsh, F J M Smulders
Effects on vacuum packaged and non-packaged beef longissimus samples exposed to atmospheric cold plasma (ACP) generated at different powers were studied over a 10day period of vacuum-, and a subsequent 3day period of aerobic storage. Exposure of non-covered beef samples under high power ACP conditions resulted in increased a*, b*, Chroma and Hue values, but ACP treatment of packaged loins did not impact colour (L*, a*, b*, Chroma, Hue), lipid peroxidation, sarcoplasmic protein denaturation, nitrate/nitrite uptake, or myoglobin isoform distribution...
February 8, 2017: Meat Science
https://www.readbyqxmd.com/read/28185802/infection-in-burn-patients-in-a-referral-center-in-colombia
#20
Carlos Enrique Ramirez-Blanco, Carlos Enrique Ramirez-Rivero, Luis Alfonso Diaz-Martinez, Luis Miguel Sosa-Avila
INTRODUCTION: Worldwide, burns are responsible for more than 300,000 deaths annually; infection is a major cause of morbidity and mortality in these patients. Early identification and treatment of infection improves outcome. Toward this end it's necessary to identify the institutions flora and organisms that most frequently produces infection. OBJECTIVES: To characterize infections developed by burn patients hospitalized at the University Hospital of Santander (HUS)...
February 6, 2017: Burns: Journal of the International Society for Burn Injuries
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