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https://www.readbyqxmd.com/read/29134942/intramammary-infusion-of-a-live-culture-of-lactococcus-lactis-in-ewes-to-treat-staphylococcal-mastitis
#1
Sebastian Alessandro Mignacca, Simone Dore, Liliana Spuria, Pietro Zanghì, Benedetta Amato, Ilaria Duprè, Federica Armas, Elena Biasibetti, Cristina Camperio, Stefano A Lollai, Maria Teresa Capucchio, Eugenia Agnese Cannas, Vincenzo Di Marco Lo Presti, Cinzia Marianelli
PURPOSE: Alternatives to antibiotic therapy for mastitis in ruminants are needed. We present an evaluation, in two trials, of the efficacy of an intramammary infusion of a live culture of Lactococcus lactis for the treatment of subclinical and clinical mastitis in ewes. METHODOLOGY: In total, 67 animals were enrolled: 19 lactating ewes (study 1), including healthy (N=6) and coagulase-negative staphylococci (CNS)-infected ewes (N=13); and 48 lactating ewes (study 2) with either CNS mastitis (N=32), or Staphylococcus aureus mastitis (N=16), for a total of 123 mammary glands...
November 14, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29111996/microbial-keratitis-after-accelerated-corneal-collagen-cross-linking-in-keratoconus
#2
Prafulla K Maharana, Pranita Sahay, M Sujeeth, Deepali Singhal, Anubha Rathi, Jeewan S Titiyal, Namrata Sharma
PURPOSE: To assess the pattern of microbial keratitis after accelerated corneal collagen cross-linkage (aCXL) in patients with keratoconus. METHOD: The medical records of cases of keratoconus that underwent aCXL from June 2014 to May 2017 were reviewed. Cases that developed microbial keratitis after aCXL were included in the study. The clinical, microbiological profile and the treatment outcomes were evaluated. RESULTS: Of 532 eyes that underwent aCXL, 7 cases developed microbial keratitis during the study period...
November 2, 2017: Cornea
https://www.readbyqxmd.com/read/29099783/metagenomic-analysis-of-therapeutic-pyo-phage-cocktails-from-1997-to-2014
#3
Julia Villarroel, Mette Voldby Larsen, Mogens Kilstrup, Morten Nielsen
Phage therapy has regained interest in recent years due to the alarming spread of antibiotic resistance. Whilst phage cocktails are commonly sold in pharmacies in countries such as Georgia and Russia, this is not the case in western countries due to western regulatory agencies requiring a thorough characterization of the drug. Here, DNA sequencing of constituent biological entities constitutes a first step. The pyophage (PYO) cocktail is one of the main commercial products of the Georgian Eliava Institute of Bacteriophage, Microbiology and Virology and is used to cure skin infections...
November 3, 2017: Viruses
https://www.readbyqxmd.com/read/29081626/ceftriaxone-for-the-treatment-of-methicillin-susceptible-staphylococcus-aureus-bacteremia-a-case-series
#4
Rachel Amanda Lowe, Katie Elizabeth Barber, Jamie Leigh Wagner, Allison Miriam Bell-Harlan, Kayla Renay Stover
Methicillin-susceptible Staphylococcus aureus (MSSA) causes 45% of S. aureus bloodstream infections (BSI) and is the most important cause of BSI-associated death. The standard of care therapy is an anti-staphylococcal penicillin or cefazolin, but dosing frequencies for these agents are often infeasible; multiple daily doses tie up infusion lines and are impractical for outpatient antibiotic infusion. Ceftriaxone represents a promising alternative, with once daily dosing and a short infusion time. Currently, treatment with ceftriaxone for invasive MSSA infections is infrequent, with minimal data supporting the clinical utility of ceftriaxone for MSSA BSI...
July 2017: Journal of Pharmacology & Pharmacotherapeutics
https://www.readbyqxmd.com/read/29077854/investigation-of-a-cluster-of-sequence-type-22-methicillin-resistant-staphylococcus-aureus-transmission-in-a-community-setting
#5
Michelle S Toleman, Emmeline R Watkins, Tom Williams, Beth Blane, Belinda Sadler, Ewan M Harrison, Francesc Coll, Julian Parkhill, Bernadette Nazareth, Nicholas M Brown, Sharon J Peacock
Background: Whole-genome sequencing (WGS) has typically been used to confirm or refute hospital/ward outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) identified through routine practice. However, appropriately targeted WGS strategies that identify routinely "undetectable" transmission remain the ultimate aim. Methods: WGS of MRSA isolates sent to a regional microbiological laboratory was performed as part of a 12-month prospective observational study...
October 25, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29071076/burden-of-bacterial-upper-respiratory-tract-pathogens-in-school-children-of-nepal
#6
Sangita Thapa, Shishir Gokhale, Annavarapu Laxminarasimha Sharma, Lokendra Bahadur Sapkota, Shamshul Ansari, Rajendra Gautam, Sony Shrestha, Puja Neopane
INTRODUCTION: Respiratory tract infections are one of the most common human infections in all age group and important cause of mortality and morbidity worldwide. Most bacterial upper respiratory tract infections are vaccine preventable. This study aimed to determine the prevalence of carrier state of bacterial upper respiratory tract pathogens among school children. It also aimed to study their antibiograms. METHODS: The specimen from posterior pharyngeal wall and tonsils were collected from 204 participants on calcium alginate coated swabs (HiMedia)...
2017: BMJ Open Respiratory Research
https://www.readbyqxmd.com/read/29070701/longitudinal-genomic-surveillance-of-mrsa-in-the-uk-reveals-transmission-patterns-in-hospitals-and-the-community
#7
Francesc Coll, Ewan M Harrison, Michelle S Toleman, Sandra Reuter, Kathy E Raven, Beth Blane, Beverley Palmer, A Ruth M Kappeler, Nicholas M Brown, M Estée Török, Julian Parkhill, Sharon J Peacock
Genome sequencing has provided snapshots of the transmission of methicillin-resistant Staphylococcus aureus (MRSA) during suspected outbreaks in isolated hospital wards. Scale-up to populations is now required to establish the full potential of this technology for surveillance. We prospectively identified all individuals over a 12-month period who had at least one MRSA-positive sample processed by a routine diagnostic microbiology laboratory in the East of England, which received samples from three hospitals and 75 general practitioner (GP) practices...
October 25, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/29065670/gentamicin-released-from-porous-scaffolds-fabricated-by-stereolithography
#8
Somruethai Channasanon, Pareeya Udomkusonsri, Surapol Chantaweroad, Passakorn Tesavibul, Siriporn Tanodekaew
Porous oligolactide-hydroxyapatite composite scaffolds were obtained by stereolithographic fabrication. Gentamicin was then coated on the scaffolds afterwards, to achieve antimicrobial delivery ability to treat bone infection. The scaffolds examined by stereomicroscope, SEM, and μCT-scan showed a well-ordered pore structure with uniform pore distribution and pore interconnectivity. The physical and mechanical properties of the scaffolds were investigated. It was shown that not only porosity but also scaffold structure played a critical role in governing the strength of scaffolds...
2017: Journal of Healthcare Engineering
https://www.readbyqxmd.com/read/29062249/a-retrospective-evaluation-of-blood-cultures-in-a-pediatric-intensive-care-unit-a-three-year-evaluation
#9
Ayşe Betül Ergül, Halit Işık, Yasemin Ay Altıntop, Yasemin Altuner Torun
AIM: In this study, it was aimed to retrospectively assess the frequency and antibiotic resistance of microorganisms isolated from blood cultures of patients in a pediatric intensive care unit. MATERIAL AND METHODS: The study was conducted on blood culture tests obtained from patients in a pediatric intensive care unit and sent to a microbiology laboratory between 2013 and 2016. The species and antibiotic susceptibilities were assessed in microorganisms isolated from the blood cultures...
September 2017: Türk Pediatri Arşivi
https://www.readbyqxmd.com/read/29057725/prevalence-of-nasal-colonization-with-staphylococcus-aureus-in-patients-with-rheumatoid-arthritis
#10
Gloria Albert, Milagros Ricse, Javier Narvaez, Jesus Rodriguez, Joan Miquel Nolla, Mari Angeles Dominguez-Luzon, Carmen Gomez Vaquero
OBJECTIVE: Patients with rheumatoid arthritis (RA) and nasal carriers of Staphylococcus aureus have an increased risk of developing infections caused by S. aureus. Our objective was to determine the prevalence of S. aureus nasal colonization in patients with RA and its relationship to RA treatments. METHODS: Two hundred seven patients with RA and 37 healthy controls were prospectively included in a cross-sectional study. A nasal secretion sample was collected by swab from both anterior nostrils and was referred to the hospital's microbiology department for culturing...
October 20, 2017: Current Rheumatology Reviews
https://www.readbyqxmd.com/read/29055511/the-relationship-between-causative-microorganisms-and-cardiac-lesions-caused-by-infective-endocarditis-new-perspectives-from-the-contemporary-cohort-of-patients
#11
Danijela Trifunovic, Bosiljka Vujisic-Tesic, Biljana Obrenovic-Kircanski, Branislava Ivanovic, Dimitra Kalimanovska-Ostric, Milan Petrovic, Marija Boricic-Kostic, Snezana Matic, Goran Stevanovic, Jelena Marinkovic, Olga Petrovic, Gordana Draganic, Mirjana Tomic-Dragovic, Svetozar Putnik, Dejan Markovic, Vladimir Tutus, Ivana Jovanovic, Maja Markovic, Ivana M Petrovic, Jelena M Petrovic, Jelena Stepanovic
BACKGROUND: The etiology of infective endocarditis (IE) is changing. More aggressive forms with multiple IE cardiac lesions have become more frequent. This study sought to explore the relationship between contemporary causative microorganisms and IE cardiac lesions and to analyze the impact of multiple lesions on treatment choice. METHODS: In 246 patients hospitalized for IE between 2008 and 2015, cardiac lesions caused by IE were analyzed by echocardiography, classified according to the 2015 European Society of Cardiology guidelines and correlated with microbiological data...
October 18, 2017: Journal of Cardiology
https://www.readbyqxmd.com/read/29051689/detection-of-newly-defined-superantigenic-toxin-genes-and-coagulase-gene-polymorphism-in-staphylococcus-aureus-isolates
#12
Bareq A Al-Ajealy, Maysa S M Al-Shukri, Hassan S Al-Jumaily
The current study aims to use coagulase (coa) polymorphism gene to identify Staphylococcus aureus isolated from stool samples, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 100 specimens were collected from patients in Babylon province, Iraq, between July 2016 and September 2016. Twenty S. aureus strains were isolated and identified using standard laboratory microbiological tests. The bacterial isolates were then examined by coa gene restriction fragment length polymorphism genotyping...
October 2017: Reviews in Medical Microbiology: a Journal of the Pathological Society of Great Britain and Ireland
https://www.readbyqxmd.com/read/29050434/microbial-loads-and-antibiotic-resistance-patterns-of-staphylococcus-aureus-in-different-types-of-raw-poultry-based-meat-preparations
#13
Laura Buzón-Durán, Rosa Capita, Carlos Alonso-Calleja
The hygiene status of raw chicken-meat preparations from retail outlets in North-Western Spain was investigated. Microbial counts (aerobic plate counts (APCs), psychrotrophs, Enterobacteriaceae, fecal coliforms, enterococci, pseudomonads, fluorescent pseudomonads, yeasts and molds, and Staphylococcus aureus) were determined for minced meat, hamburgers, nuggets, white sausages, red sausages, escalope, and roll-ups. S. aureus isolates were tested for susceptibility to twenty antimicrobials of veterinary and human clinical significance (disc diffusion method, CLSI)...
September 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/29045009/trends-in-non-lactation-breast-abscesses-in-a-tertiary-hospital-setting
#14
Apoorva Saboo, Ian Bennett
BACKGROUND: The aim of this paper was to retrospectively review non-lactation breast abscesses treated in Princess Alexandra Hospital over a 10-year period and to illustrate the trends in size, risk factors, microbiological profile and management. METHODS: A computerized search of the medical records was undertaken to identify all adult patients with a diagnosis of breast abscesses during June 2005-June 2015. Patients concurrently breastfeeding were excluded. A retrospectively review of the clinical records was performed...
October 18, 2017: ANZ Journal of Surgery
https://www.readbyqxmd.com/read/29016585/isolation-of-multidrug-resistant-bacteria-from-patients-medical-charts
#15
Roshan Thapa, Narayan Dutt Pant, Uday Narayan Yadav, Eliza Thapa, Anjana Singh, Bharat Mani Pokhrel, Upendra Devkota
BACKGROUND: Patient's medical charts in hospitals are potentially contaminated by pathogenic bacteria and might act as vehicles for transmission of bacterial infections.This study was aimed to determine the rate of contamination of medical charts by multidrug resistant bacteria. METHODS: Sampling of total 250 patient's medical charts from different wards was done with the help of cotton swabs soaked in sterile normal saline. The swabs thus collected were cultured using standard microbiological procedures...
September 8, 2017: Journal of Nepal Health Research Council
https://www.readbyqxmd.com/read/28993066/clinical-and-microbiological-profile-of-infectious-keratitis-in-an-area-of-madrid-spain
#16
Soraya Mediero, Ana Boto de Los Bueis, Karina Spiess, Mariana Díaz-Almirón, Almudena Del Hierro Zarzuelo, Isabel Villalaín Rodes, Adela García Perea
INTRODUCTION: To study antibiotic susceptibility in bacterial keratitis (BK), its profile over 10 years and its influence on ophthalmological practice. METHODS: Retrospective review of BK with positive corneal scraping over a 10-year period. Risk factors for keratitis, visual acuity (VA), empirical topical treatment, corneal infection characteristics and outcomes were analyzed for BK due to Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Propionibacterium acnes...
October 6, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28980591/-the-role-of-persistent-viral-infections-with-herpes-viridae-and-papilloma-viridae-associated-with-chronic-inflammatory-pathology-of-the-oropharynx
#17
N D Yushchuk, A I Kryukov, A B Turovsky, I A Popova, M V Savostikova
The high prevalence of chronic inflammatory oropharyngeal pathologies and a large variety of specific pathogenetic features of the persistent viral infections caused by the species of the families Herpesviridae and Papillomaviridae as etiological agents of the disease suggest the necessity of investigations with a view to evaluating the clinical significance of persistent viral infections with Herpesviridae and Papillomaviridae species in the patients presenting with chronic inflammatory oropharyngreal pathology...
2017: Vestnik Otorinolaringologii
https://www.readbyqxmd.com/read/28965794/health-care-associated-infections-in-pre-transplant-liver-intensive-care-unit-perspectives-and-challenges
#18
Elham A Hassan, Nahla M Elsherbiny, Abeer S Abd El-Rehim, Asmaa M A Soliman, Asmaa O Ahmed
BACKGROUND: Health care-associated infections (HAIs) threaten patient's safety worldwide especially in the intensive care units (ICU). In end-stage liver disease (ESLD), the condition is much more complicated. Data regarding HAIs among ESLD patients is lacking. We aimed to assess the incidence of HAIs, risk factors, causative micro-organisms, antimicrobial susceptibilities and mortality rates among patients with end-stage liver disease (ESLD) admitted to pre-transplant liver intensive care unit (LICU)...
September 28, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28956540/molecular-characteristics-and-antibiotic-resistance-pattern-of-staphylococcus-aureus-nasal-carriage-in-tertiary-care-hospitals-of-isfahan-iran
#19
Malihe Karimi, Bahram Nasr Esfahani, Mehrdad Halaji, Sina Mobasherizadeh, Mojtaba Shahin, Seyed Rohollah Havaei, Dariush Shokri, Seyed Asghar Havaei
Carriage of S. aureus in the anterior nares seems to play a significant role in the pathogenesis of infection. This study aimed to determine the molecular characteristics and antibiotic susceptibility pattern of S. aureus isolates obtained from the nasal carriage of health care workers (HCWs). This study was performed during July 2014 to July 2015 at three tertiary care hospitals. Nasal samples were collected from the nasal cavity of HCWs. Standard microbiological methods were used for identification of S. aureus isolates...
September 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/28935494/-an-atypical-case-of-necrotizing-staphylococcal-pneumonia
#20
E Bertreau, S Grard, T Baudry, N Freymond
INTRODUCTION: Some strains of Staphylococcus aureus produce a toxin known as Panton-Valentine leukocidin. These strains notably cause a necrotizing pneumonia which is associated with a high mortality. OBSERVATION: A 70-year-old woman presented with sub-acute onset dyspnea, low-grade fever, and hemoptysis after a trip to Dubai and New Zealand. Computed tomography showed bilateral necrotizing pneumonia, suggesting the diagnosis of pneumonia caused by S. aureus producing Panton-Valentine toxin...
September 18, 2017: Revue des Maladies Respiratoires
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