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Metal plant defense

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https://www.readbyqxmd.com/read/29331014/biochemical-characterization-of-recombinant-thermostable-cohnella-sp-a01-%C3%AE-glucanase
#1
Meysam Rezaie, Saeed Aminzadeh, Farid Heidari, Masoud Mashhadi Akbar Boojar, Ali Asghar Karkhane
Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are release by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M) from native thermophilic bacteria, Cohnella A01. Methods: bg16M gene was cloned and expressed in E. coli BL21 (DE3)...
January 13, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29296983/enhanced-structural-diversity-in-terpenoid-biosynthesis-enzymes-substrates-and-cofactors
#2
REVIEW
Abith Vattekkatte, Stefan Garms, Wolfgang Brandt, Wilhelm Boland
The enormous diversity of terpenes found in nature is generated by enzymes known as terpene synthases, or cyclases. Some are also known for their ability to convert a single substrate into multiple products. This review comprises monoterpene and sesquiterpene synthases that are multiproduct in nature along with the regulation factors that can alter the product specificity of multiproduct terpene synthases without genetic mutations. Variations in specific assay conditions with focus on shifts in product specificity based on change in metal cofactors, assay pH and substrate geometry are described...
January 3, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29275195/nitric-oxide-mediated-integrative-alterations-in-plant-metabolism-to-confer-abiotic-stress-tolerance-no-crosstalk-with-phytohormones-and-no-mediated-post-translational-modifications-in-modulating-diverse-plant-stress
#3
REVIEW
Fareen Sami, Mohammad Faizan, Ahmad Faraz, Husna Siddiqui, Mohammad Yusuf, Shamsul Hayat
Nitric oxide (NO) is a major signaling biomolecule associated with signal transduction in plants. The beneficial role of NO in plants, exposed to several abiotic stresses shifted our understanding as it being not only free radical, released from the toxic byproducts of oxidative metabolism but also helps in plant sustenance. An explosion of research in plant NO biology during the last two decades has revealed that NO is a key signal associated with plant growth, germination, photosynthesis, leaf senescence, pollen growth and reorientation...
December 21, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/29247887/protective-role-of-exogenous-phytohormones-on-redox-status-in-pea-seedlings-under-copper-stress
#4
Marouane Ben Massoud, Lamia Sakouhi, Inès Karmous, Yao Zhu, Ezzedine El Ferjani, David Sheehan, Abdelilah Chaoui
The present work aims to provide insight on the role of phytohormone application in developing efficient practical defense strategies to improve plants tolerance under heavy metal contamination. For this purpose, pea (Pisum sativum L.) seeds were germinated in an aqueous solution of 200μM CuCl2 up to the 3rd day and then continued to germinate in the presence of distilled water (stress cessation) or were subjected to following combinations: Cu+1μM IAA and Cu+1μM GA3 for 3 additional days. The results showed that copper excess induced oxidative stress in germinating seeds, which resulted in changes of the redox state of glutathione and cysteine, and proteomics revealed Cu-induced modifications of thiols (SH) and carbonyls (CO) (indicators of protein oxidation)...
December 9, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29232589/peptides-having-antimicrobial-activity-and-their-complexes-with-transition-metal-ions
#5
REVIEW
Małgorzata Jeżowska-Bojczuk, Kamila Stokowa-Sołtys
Peptide antibiotics are produced by bacterial, mammalian, insect or plant organisms in defense against invasive microbial pathogens. Therefore, they are gaining importance as anti-infective agents. There are a number of antibiotics that require metal ions to function properly. Metal ions play a key role in their action and are involved in specific interactions with proteins, nucleic acids and other biomolecules. On the other hand, it is well known that some antimicrobial agents possess functional groups that enable them interacting with metal ions present in physiological fluids...
December 2, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29192439/-cloning-and-expression-analysis-of-transcription-factor-asmyb1-and-asmyb2-from-aquilaria-sinensis
#6
Ying-Ying Feng, Xian-Juan Dong, Xiao Liu, Ya-Ru Yan, Jin-Ling Wang, She-Po Shi, Xiao-Hui Wang, Peng-Fei Tu
The MYB gene family comprises one of the richest groups of transcription factors in plants. The full length of two MYB genes were isolated through heterologous screening of Aquilaria sinensis calli transcriptome data, and the reverse transcription PCR was performed to obstain the corrected MYB clones, named AsMYB1, AsMYB2. The MYB transmembrane domain and phylogenetic analysis were predicted by different software to analyze the bioinformatics of MYB proteins. The transcript level of AsMYB1, AsMYB2 was performed by real-time quantitative RT-PCR in different tissues and in responds to abiotic stresses including salt, cold, metal and drought stress, and hormone treatments including abscisic acid (ABA), salicylic acid (SA), gibberellins (GA3) and methyl jasmonate (MeJA) treatment...
September 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29183602/copper-complexes-of-the-1-3-4-thiadiazole-derivatives-modulate-antioxidant-defense-responses-and-resistance-in-tomato-plants-against-fungal-and-bacterial-diseases
#7
Amal Smaili, Lalla Aicha Rifai, Siham Esserti, Tayeb Koussa, Fouad Bentiss, Salaheddine Guesmi, Abdelhakim Laachir, Mohamed Faize
The metallic complexes μ-chloro-μ-[2,5-bis (2-pyridyl)-1,3,4-thiadiazole] aqua chlorocopper (II) dichlorocopper (II) (abbreviated 2PTH-Cu2-Cl4); aquabis [2,5-bis (2-pyridyl)-1,3,4-thiadiazole-κ2N2,N3] (trifluoromethane-sulfonato-κO) copper(II) trifluoro metrhanesulfonate (2PTH-Cu-tF) and bis[(2,5-bis(pyridine-2-yl)-1,3,4-thiadiazole-di-azido copper(II)] (2PTH-Cu-Az) were compared for their antimicrobial activities in vitro, and their aptitude to control Verticillium wilt and crown gall diseases development of tomato in the greenhouse...
November 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29163398/phytohormones-and-beneficial-microbes-essential-components-for-plants-to-balance-stress-and-fitness
#8
REVIEW
Dilfuza Egamberdieva, Stephan J Wirth, Abdulaziz A Alqarawi, Elsayed F Abd Allah, Abeer Hashem
Plants are subjected to various abiotic stresses, such as drought, extreme temperature, salinity, and heavy metals. Abiotic stresses have negative impact on the physiology and morphology of plants through defects in the genetic regulation of cellular pathways. Plants employ several tolerance mechanisms and pathways to avert the effects of stresses that are triggered whenever alterations in metabolism are encountered. Phytohormones are among the most important growth regulators; they are known for having a prominent impact on plant metabolism, and additionally, they play a vital role in the stimulation of plant defense response mechanisms against stresses...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29111597/responses-of-an-adventitious-fast-growing-plant-to-photodynamic-stress-comparative-study-of-anionic-and-cationic-porphyrin-effect-on-arabidopsis-thaliana
#9
Mohammad Issawi, Damien Guillaumot, Vincent Sol, Catherine Riou
Antimicrobial Photodynamic Treatment based on the use of a photosensitizer to produce reactive oxygen species that induce cell death could be envisaged to fight against plant pathogens. For setting this strategy, we want to study how plants themselves respond to photodynamic treatment. In a previous work we showed that tomato plantlets were able to resist to photoactivated tetra (N-methylpyridyl) porphyrin (CP) or Zinc metalated form (CP-Zn). To enlarge our plant expertise related to exogenous porphyrins treatment and to further defend this approach, we studied how an adventice like Arabidopsis thaliana responded to exogenous supply of anionic and cationic porphyrins...
November 7, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29091834/assessment-of-edibility-and-effect-of-arbuscular-mycorrhizal-fungi-on-solanum-melongena-l-grown-under-heavy-metal-loid-contaminated-soil
#10
Ritu Chaturvedi, Paulo Favas, João Pratas, Mayank Varun, Manoj S Paul
Arbuscular mycorrhizal fungi (AMF) aids in plant establishment at heavy metal(loid) (HM) contaminated soils, strengthening plant defense system along with promoting growth. A pot experiment was carried out to evaluate the effect of AMF on eggplants grown under HM stress. Further, the potential health risks of HM exposure to the humans via dietary intake of eggplant were also estimated. Results showed that AMF application improved growth, biomass and antioxidative defense response of plants against HM stress...
October 27, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29089035/transcriptional-analysis-of-sweet-orange-trees-co-infected-with-candidatus-liberibacter-asiaticus-and-mild-or-severe-strains-of-citrus-tristeza-virus
#11
Shimin Fu, Jonathan Shao, Cristina Paul, Changyong Zhou, John S Hartung
BACKGROUND: Citrus worldwide is threatened by huanglongbing (HLB) and tristeza diseases caused by 'Candidatus Liberibacter asiaticus' (CaLas) and Citrus tristeza virus (CTV). Although the pathogens are members of the α-proteobacteria and Closteroviridae, respectively, both are restricted to phloem cells in infected citrus and are transmitted by insect vectors. The response of sweet orange to single infection by either of these two pathogens has been characterized previously by global gene expression analysis...
October 31, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29028613/divergent-biology-of-facultative-heavy-metal-plants
#12
REVIEW
Hermann Bothe, Aneta Słomka
Among heavy metal plants (the metallophytes), facultative species can live both in soils contaminated by an excess of heavy metals and in non-affected sites. In contrast, obligate metallophytes are restricted to polluted areas. Metallophytes offer a fascinating biology, due to the fact that species have developed different strategies to cope with the adverse conditions of heavy metal soils. The literature distinguishes between hyperaccumulating, accumulating, tolerant and excluding metallophytes, but the borderline between these categories is blurred...
September 7, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28965600/evaluation-of-silicon-influence-on-the-mitigation-of-cadmium-stress-in-the-development-of-arabidopsis-thaliana-through-total-metal-content-proteomic-and-enzymatic-approaches
#13
Josiane M T Carneiro, Katherine Chacón-Madrid, Rodrigo M Galazzi, Bruna K Campos, Sandra C C Arruda, Ricardo A Azevedo, Marco A Z Arruda
The mitigation of Cd-stress through Si addition to Arabidopsis thaliana cultivation is evaluated in terms of total metal content, proteomic and enzymatic approaches. Four different treatment are evaluated: TC (control, without Si or Cd addition), T1 (with Si addition), T2 (with Cd addition), and T3 (with Si and Cd addition). Through the total determination of Cd and Si in Arabidopsis leaves, the Cd concentration decreased by half when T2 is compared with T3 treatment. In terms of proteomic approach, some differential protein species are achieved by comparative proteomics through 2-D DIGE of all treatments evaluated...
December 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/28951982/effects-of-cd-zn-or-pb-stress-in-populus-alba-berolinensis-on-the-development-and-reproduction-of-lymantria-dispar
#14
Dun Jiang, Shanchun Yan
In order to investigate the effects of heavy metal stress on woody plant defense against phytophagous insects, we studied development and reproduction traits of the gypsy moth, Lymantria dispar that were separately fed with leaves plucked from poplar seedlings (Populus alba berolinensis) grown in either non-contaminated soil (control), Cd-contaminated soil (1.5 mg/kg), Zn-contaminated soil (500 mg/kg) or Pb-contaminated soil (500 mg/kg). The results showed that feeding on Cd or Pb stressed poplar leaves significantly decreased L...
September 26, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28942275/castasterone-confers-copper-stress-tolerance-by-regulating-antioxidant-enzyme-responses-antioxidants-and-amino-acid-balance-in-b-juncea-seedlings
#15
Poonam Yadav, Ravdeep Kaur, Mukesh Kumar Kanwar, Anket Sharma, Vinod Verma, Geetika Sirhindi, Renu Bhardwaj
The aim of the present study was to explore the effect of exogenous application of castasterone (CS) on physiologic and biochemical responses in Brassica juncea seedlings under copper (Cu) stress. Seeds were pre-soaked in different concentrations of CS and grown for 7 days under various levels of Cu. The exposure of B. juncea to higher levels of Cu led to decrease of morphologic parameters, with partial recovery of length and fresh weight in the CS pre-treated seedlings. Metal content was high in both roots and shoots under Cu exposure while the CS pre-treatment reduced the metal uptake...
January 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28932199/bacillus-a-biological-tool-for-crop-improvement-through-bio-molecular-changes-in-adverse-environments
#16
REVIEW
Ramalingam Radhakrishnan, Abeer Hashem, Elsayed F Abd Allah
Crop productivity is affected by environmental and genetic factors. Microbes that are beneficial to plants are used to enhance the crop yield and are alternatives to chemical fertilizers and pesticides. Pseudomonas and Bacillus species are the predominant plant growth-promoting bacteria. The spore-forming ability of Bacillus is distinguished from that of Pseudomonas. Members of this genus also survive for a long time under unfavorable environmental conditions. Bacillus spp. secrete several metabolites that trigger plant growth and prevent pathogen infection...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28926584/transcriptome-analysis-reveals-molecular-anthelmintic-effects-of-procyanidins-in-c-elegans
#17
Verena Spiegler, Andreas Hensel, Jochen Seggewiß, Milena Lubisch, Eva Liebau
Worldwide, more than 1 billion people are affected by infestations with soil-transmitted helminths and also in veterinary medicine helminthiases are a severe threat to livestock due to emerging resistances against the common anthelmintics. Proanthocyanidins have been increasingly investigated for their anthelmintic properties, however, except for an interaction with certain proteins of the nematodes, not much is known about their mode of action. To investigate the anthelmintic activity on a molecular level, a transcriptome analysis was performed in Caenorhabditis elegans after treatment with purified and fully characterized oligomeric procyanidins (OPC)...
2017: PloS One
https://www.readbyqxmd.com/read/28924722/evaluation-of-heme-oxygenase-1-ho-1-in-cd-and-ni-induced-cytotoxicity-and-crosstalk-with-ros-quenching-enzymes-in-two-to-four-leaf-stage-seedlings-of-vigna-radiata
#18
Lovely Mahawar, Rajesh Kumar, Gyan Singh Shekhawat
Research on heme oxygenase in plants has received consideration in recent years due to its several roles in development, defense, and metabolism during various environmental stresses. In the current investigation, the role of heme oxygenase (HO) 1 was evaluated in reducing heavy metal (Cd and Ni) uptake and alleviating Cd and Ni toxicity effects in the hydroponically grown seedlings of Vigna radiata var. PDM 54. Seedlings were subjected to Cd- and Ni-induced oxidative stress independently at different concentrations ranging from 10 to 100 μM...
September 18, 2017: Protoplasma
https://www.readbyqxmd.com/read/28912786/co-infection-of-sweet-orange-with-severe-and-mild-strains-of-citrus-tristeza-virus-is-overwhelmingly-dominated-by-the-severe-strain-on-both-the-transcriptional-and-biological-levels
#19
Shimin Fu, Jonathan Shao, Changyong Zhou, John S Hartung
Citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted Closterovirus, Citrus tristeza virus. Mild strain CTV-B2 does not cause obvious symptoms on indicators whereas severe strain CTV-B6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. Our laboratory has previously characterized changes in transcription in sweet orange separately infected with CTV-B2 and CTV-B6. In the present study, transcriptome analysis of Citrus sinensis in response to double infection by CTV-B2 and CTV-B6 was carried out...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28881317/modulation-of-antioxidative-defense-expression-and-osmolyte-content-by-co-application-of-24-epibrassinolide-and-salicylic-acid-in-pb-exposed-indian-mustard-plants
#20
Sukhmeen Kaur Kohli, Neha Handa, Shagun Bali, Saroj Arora, Anket Sharma, Ravdeep Kaur, Renu Bhardwaj
The study focuses on potential of combined pre-soaking treatment of 24-Epibrassinolide (EBL) and Salicylic acid (SA) in alleviating Pb phytotoxicity in Brassica juncea L. plants. The seeds after treatment with combination of both the hormones were sown in mixture of soil, sand and manure (3:1:1) and were exposed to Pb concentrations (0.25mM, 0.50mM and 0.75mM). After 30 days of growth, the plants were harvested and processed, for quantification of various metabolites. It was found that pre-sowing of seeds in combination of EBL and SA, mitigated the adverse effects of metal stress by modulating antioxidative defense response and enhanced osmolyte contents...
January 2018: Ecotoxicology and Environmental Safety
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