Haoyan Ji, Zhuoyue Bi, Aashna S Pawar, Akimasa Seno, Bandar Saeed Almutairy, Yao Fu, Yiran Qiu, Wenxuan Zhang, Ziwei Wang, Chitra Thakur, Hongjuan Cui, Liqun Yang, Fei Chen
As one of the first identified oncogenic microRNAs, the precise details concerning the transcriptional regulation and function of microRNA-21 (miR-21) are still not completely established. The miR-21 gene is situated on chromosome 17q23.2, positioned at the 3'-UTR of the gene that encodes vacuole membrane protein-1 (VMP1). In this current study, we presented evidence indicating that miR-21 possesses its own gene promoter, which can be found in the intron 10 of the VMP1 gene. Chromatin immunoprecipitation followed by global DNA sequencing (ChIP-seq) revealed the presence of a broad H3K4me3 peak spanning the entire gene body of the primary miR-21 and the existence of super-enhancer clusters in the close proximity to both the miR-21 gene promoter and the transcription termination site in arsenic (As3+ )-induced cancer stem-like cells (CSCs) and human induced pluripotent stem cells (hiPSCs)...
January 29, 2024: Environmental Pollution