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https://www.readbyqxmd.com/read/29666624/recombinant-protein-containing-b-cell-epitopes-of-different-loxosceles-spider-toxins-generates-neutralizing-antibodies-in-immunized-rabbits
#1
Sabrina de Almeida Lima, Clara Guerra-Duarte, Fernanda Costal-Oliveira, Thais Melo Mendes, Luís F M Figueiredo, Daysiane Oliveira, Ricardo A Machado de Avila, Valéria Pereira Ferrer, Dilza Trevisan-Silva, Silvio S Veiga, João C Minozzo, Evanguedes Kalapothakis, Carlos Chávez-Olórtegui
Loxoscelism is the most important form of araneism in South America. The treatment of these accidents uses heterologous antivenoms obtained from immunization of production animals with crude loxoscelic venom. Due to the scarcity of this immunogen, new alternatives for its substitution in antivenom production are of medical interest. In the present work, three linear epitopes for Loxosceles astacin-like protease 1 (LALP-1) (SLGRGCTDFGTILHE, ENNTRTIGPFDYDSIMLYGAY, and KLYKCPPVNPYPGGIRPYVNV) and two for hyaluronidase (LiHYAL) (NGGIPQLGDLKAHLEKSAVDI and ILDKSATGLRIIDWEAWR) from Loxosceles intermedia spider venom were identified by SPOT-synthesis technique...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29666547/use-of-urea-polyacrylamide-electrophoresis-for-discrimination-of-a1-and-a2-beta-casein-variants-in-raw-cow-s-milk
#2
Miguel Angel Duarte-Vázquez, Carlos Raúl García-Ugalde, Blanca Erika Álvarez, Laura Mariana Villegas, Blanca Estela García-Almendárez, Jorge Luis Rosado, Carlos Regalado
Beta-casein (BC) in cow's milk occurs in several genetic variants, where BC A1 (BCA1) and BC A2 (BCA2) are the most frequent. This work deals with a method based on modified polyacrylamide gel electrophoresis using urea PAGE to discriminate BCA1 and BCA2 variants from Holstein Friesian (HF) and genetically selected Jersey A2/A2 (JA2) cow's milk. Two well defined bands were obtained from BC fraction of HF milk, while that of JA2 showed a single band. Proteins from these bands were sequenced by HPLC-quadrupole linear ion trap/mass spectrometry, resulting in BCA1 and BCA2 separation from the BC fraction of HF milk, whereas BCA2 was the only constituent of JA2 fraction...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665843/next-generation-sequencing-analysis-of-receptor-type-tyrosine-kinase-genes-in-surgically-resected-colon-cancer-identification-of-gain-of-function-mutations-in-the-ret-proto-oncogene
#3
Duarte Mendes Oliveira, Katia Grillone, Chiara Mignogna, Valentina De Falco, Carmelo Laudanna, Flavia Biamonte, Rosa Locane, Francesco Corcione, Massimiliano Fabozzi, Rosario Sacco, Giuseppe Viglietto, Donatella Malanga, Antonia Rizzuto
BACKGROUND: Improvement in genetic characterization of Colon Cancer (CC) patients is required to propose new potential targets, since surgical resection coupled to chemotherapy, presents several limits such as cancer recurrence and drug resistance. Targeted therapies have more efficacy and less toxicity than standard treatments. One of the most relevant cancer-specific actionable targets are receptor tyrosine kinases (RTKs) whose role in CC need to be better investigated. METHODS: We have analysed 37 CC patients using the Ion AmpliSeq™ Comprehensive Cancer Panel (CCP)...
April 17, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29665541/evaluation-of-a-single-extraction-test-to-estimate-the-human-oral-bioaccessibility-of-potentially-toxic-elements-in-soils-towards-more-robust-risk-assessment
#4
S M Rodrigues, N Cruz, L Carvalho, A C Duarte, E Pereira, A G F Boim, L R F Alleoni, P F A M Römkens
Intake of soil by children and adults is a major exposure pathway to contaminants including potentially toxic elements (PTEs). However, only the fraction of PTEs released in stomach and intestine are considered as bioaccessible and results from routine analyses of the total PTE content in soils, therefore, are not necessarily related to the degree of bioaccessibility. Experimental methods to determine bioaccessibility usually are time-consuming and relatively complicated in terms of analytical procedures which limits application in first tier assessments...
April 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29664149/evidence-for-an-urethro-vesical-crosstalk-mediated-by-serotonin
#5
Ana Coelho, Raquel Oliveira, Helena Cavaleiro, Celia Duarte Cruz, Francisco Cruz
AIM: To study the contribution of urethral serotonin for the urethro-vesical crosstalk METHODS: Urethane-anesthetized female rats and TPH1-/- mice underwent isovolumetric or urethral-opened cystometries during intravesical or intraurethral infusion of saline or serotonin solutions. Human and rat bladders and urethrae were immunoreacted against serotonin and neuronal markers. Serotonin concentration and TPH1 mRNA were determined in rat tissue by HPLC and qPCR. RESULTS: In rats, under isovolumetric conditions, intraurethral serotonin infusion, but not saline, evoked bladder contractions...
April 17, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/29663097/the-behaviour-of-the-nematode-steinernema-feltiae-nematoda-steinernematidae-in-sand-contaminated-with-the-industrial-pollutant-chromium-vi
#6
Stephen Boyle, Thomais Kakouli-Duarte
This study set out to determine the suitability of the nematode Steinernema feltiae as a bioindicator for heavy metal pollution, specifically chromium VI. Nematodes were introduced into sand contaminated with concentrations of Cr VI+ , in a range between 10 and 100 ppm, in increments of 10. Reproductive potential, development times and infectivity vs exposure times to Cr VI were employed as endpoints. It was observed that infective juveniles (IJ) from this nematode can survive and successfully infect host insects in the presence of Cr VI for as much as 13 days, and that the nematode increases its reproductive potential at concentrations up to 100 ppm Cr VI+ ...
April 16, 2018: Ecotoxicology
https://www.readbyqxmd.com/read/29661675/burnt-sugarcane-harvesting-is-associated-with-rhinitis-symptoms-and-inflammatory-markers
#7
Iara Buriola Trevisan, Ubiratan de Paula Santos, Marceli Rocha Leite, Aline Duarte Ferreira, Bruna Spolador de Alencar Silva, Ana Paula Coelho Figueira Freire, Gabriel Faustino Santa Brigida, Ercy Mara Cipulo Ramos, Dionei Ramos
INTRODUCTION: Burnt sugarcane harvesting requires intense physical exertion in an environment with high temperatures and exposure to particulate matter. OBJECTIVE: To evaluate the effects of burnt sugarcane harvesting on rhinitis symptoms and inflammatory markers in sugarcane workers. METHODS: 32 male sugarcane workers were evaluated with questionnaire for rhinitis symptoms, and for inflammatory markers on peripheral blood and nasal lavage, in the non-harvesting, and 3 and 6 months into the sugarcane harvesting period...
April 5, 2018: Brazilian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/29661436/model-for-end-stage-liver-disease-score-before-hepatic-transplantation-and-root-mean-square-of-the-diaphragmatic-domes-affect-postoperative-extubation-time
#8
R P Duarte, A M O da Silva, R M Tonella, L S R Ratti, I F S F Boin
INTRODUCTION: The liver transplantation procedure, in addition to its prolonged surgical time, also predisposes to complications such as changes in respiratory mechanics, lung volumes, and gas exchange. OBJECTIVE: This study aims to verify if clinical factors related to the recipient, namely immediate pretransplant Model for End-Stage Liver Disease (MELD) score, surgical time, and root square metric (RMS) of the diaphragmatic domes, affect the extubation time after liver transplantation...
April 2018: Transplantation Proceedings
https://www.readbyqxmd.com/read/29659601/in-vitro-assessment-of-cytotoxic-activities-of-lachesis-muta-muta-snake-venom
#9
Stephanie Stransky, Fernanda Costal-Oliveira, Letícia Lopes-de-Souza, Clara Guerra-Duarte, Carlos Chávez-Olórtegui, Vania Maria Martin Braga
Envenomation by the bushmaster snake Lachesis muta muta is considered severe, characterized by local effects including necrosis, the main cause of permanent disability. However, cellular mechanisms related to cell death and tissue destruction, triggered by snake venoms, are poorly explored. The purpose of this study was to investigate the cytotoxic effect caused by L. m. muta venom in normal human keratinocytes and to identify the cellular processes involved in in cellulo envenomation. In order to investigate venom effect on different cell types, Alamar Blue assay was performed to quantify levels of cellular metabolism as a readout of cell viability...
April 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29659365/-association-between-diabetic-ketoacidosis-and-acromegaly
#10
Paloma Ocampo, Juan Manuel Duarte, Ricardo Barcia, Cecilia Arévalo
Diabetes mellitus occurs in nearly 10% of patients with acromegaly and is secondary to insulin resistance caused by high levels of growth hormone. Diabetes ketoacidosis has been described as a rare complication of acromegaly, resulting from a relative insulin deficiency caused by growth hormone excess. We described the case of a 38 year-old man who presented to the emergency room with a 6-week history of polydipsia, polyuria, polyphagia and weight loss. He also had nausea, vomiting and abdominal pain from two days before admission...
2018: Medicina
https://www.readbyqxmd.com/read/29659308/the-evaluation-of-non-use-of-the-upper-limb-in-chronic-hemiparesis-is-influenced-by-the-level-of-motor-impairment-and-difficulty-of-the-activities-proposal-of-a-new-version-of-the-motor-activity-log
#11
Erika Shirley Moreira Silva, Natalia Duarte Pereira, Anna Carolyna Lepesteur Gianlorenço, Paula Rezende Camargo
This study aims to evaluate the influence of the degree of difficulty of the activities in Motor Activity Log (MAL) scores for patients with mild, moderate, and severe hemiparesis, and to estimate the correlation between motor impairment levels of hemiparesis and MAL scores in post-stroke patients. Sixty-six patients with chronic hemiparesis (49 with mild-moderate hemiparesis, and 17 with severe hemiparesis) were evaluated by the Fugl-Meyer upper-limb section and versions of MAL for different degrees of motor impairment...
April 16, 2018: Physiotherapy Theory and Practice
https://www.readbyqxmd.com/read/29656282/intrapopulation-chromosomal-polymorphism-in-mazama-gouazoubira-cetartiodactyla-cervidae-the-emergence-of-a-new-species
#12
Mirela P Valeri, Iara M Tomazella, José M B Duarte
Mazama gouazoubira is a small deer species widely distributed in South America. Previous studies have shown that this species presents intraspecific chromosomal polymorphisms, which could affect fertility due to the effects of chromosomal rearrangements on gamete formation. Important aspects regarding the karyotype evolution of this species and the genus remain undefined due to the lack of information concerning the causes of this chromosomal variation. Nineteen individuals belonging to the Mazama gouazoubira population located in the Pantanal were cytogenetically evaluated...
April 14, 2018: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29653137/hyperactivity-and-memory-learning-deficits-evoked-by-developmental-exposure-to-nicotine-and-or-ethanol-are-mitigated-by-camp-and-cgmp-signaling-cascades-activation
#13
Yael Abreu-Villaça, Anna C Carvalho-Graça, Gabriela Skinner, Bruna M Lotufo, Vitor H S Duarte-Pinheiro, Anderson Ribeiro-Carvalho, Alex C Manhães, Claudio C Filgueiras
Pregnant smoking women are frequently episodic drinkers. Here, we investigated whether ethanol exposure restricted to the brain growth spurt period when combined with chronic developmental exposure to nicotine aggravates memory/learning deficits and hyperactivity, and associated cAMP and cGMP signaling disruption. To further investigate the role of these signaling cascades, we verified whether vinpocetine (a phosphodiesterase inhibitor) ameliorates the neurochemical and behavioral outcomes. Swiss mice had free access to nicotine (NIC, 50 μg/ml) or water to drink during gestation and until the 8th postnatal day (PN8)...
April 10, 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29651747/tauroursodeoxycholic-acid-improves-motor-symptoms-in-a-mouse-model-of-parkinson-s-disease
#14
Alexandra Isabel Rosa, Sara Duarte-Silva, Anabela Silva-Fernandes, Maria João Nunes, Andreia Neves Carvalho, Elsa Rodrigues, Maria João Gama, Cecília Maria Pereira Rodrigues, Patrícia Maciel, Margarida Castro-Caldas
Parkinson's disease (PD) is characterized by severe motor symptoms, and currently there is no treatment that retards disease progression or reverses damage prior to the time of clinical diagnosis. Tauroursodeoxycholic acid (TUDCA) is neuroprotective in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD; however, its effect in PD motor symptoms has never been addressed. In the present work, an extensive behavior analysis was performed to better characterize the MPTP model of PD and to evaluate the effects of TUDCA in the prevention/improvement of mice phenotype...
April 12, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29651182/first-report-of-a-new-isolate-of-metarhizium-rileyi-from-maize-fields-of-quivic%C3%A3-n-cuba
#15
Sandra Pérez Álvarez, Amaury Méndez Guerrero, Bernardo Nayar Débora Duarte, Marco Antonio Magallanes Tapia, Jesús Alicia Chávez Medina, Yoannis Domínguez Rodríguez
Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S . frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29650326/the-plant-target-of-rapamycin-kinase-a-connector-between-sulfur-and-growth
#16
Céline Forzani, Gustavo Turqueto Duarte, Christian Meyer
Sulfur is an essential macronutrient for plants that is incorporated into sulfur-containing amino acids or metabolites crucial for plant growth and stress adaptation. A recent publication shows a connection between sulfur sensing, growth processes, and the conserved eukaryotic target of rapamycin (TOR) kinase signaling pathway.
April 10, 2018: Trends in Plant Science
https://www.readbyqxmd.com/read/29649751/orthogonal-typing-methods-identify-genetic-diversity-among-belgian-campylobacter-jejuni-strains-isolated-over-a-decade-from-poultry-and-cases-of-sporadic-human-illness
#17
Mohamed Elhadidy, Hector Arguello, Avelino Álvarez-Ordóñez, William G Miller, Alexandra Duarte, Delphine Martiny, Marie Hallin, Olivier Vandenberg, Katelijne Dierick, Nadine Botteldoorn
Campylobacter jejuni is a zoonotic pathogen commonly associated with human gastroenteritis. Retail poultry meat is a major food-related transmission source of C. jejuni to humans. The present study investigated the genetic diversity, clonal relationship, and strain risk-analysis of 403 representative C. jejuni isolates from chicken broilers (n = 204) and sporadic cases of human diarrhea (n = 199) over a decade (2006-2015) in Belgium, using multilocus sequence typing (MLST), PCR binary typing (P-BIT), and identification of lipooligosaccharide (LOS) biosynthesis locus classes...
April 4, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29649724/evaluation-of-upper-limb-movements-in-children-with-down-s-syndrome-a-systematic-review
#18
REVIEW
Jamile Benite Palma Lopes, Renata Calhes Franco de Moura, Roberta Delasta Lazzari, Natalia de Almeida Carvalho Duarte, Arislander Jonathan Lopes Dumont, Claudia Santos Oliveira
The aim of the present study was to perform a review of the literature on current quantitative clinical methods for the evaluation of upper limb movements in children and adolescents with Down syndrome, with a focus on describing the variables, protocols, motor function and motor control. METHODS: A survey of PubMed, Scielo, BVS Bireme and PEDro databases using the following key words: upper limb and EMG and Down syndrome; upper limb and kinematics and Down syndrome; upper limb and motion analysis and Down syndrome; movement and upper limb and Down syndrome; upper limb and Down syndrome; reach and Down syndrome...
April 9, 2018: Infant Behavior & Development
https://www.readbyqxmd.com/read/29648962/communication-of-scientific-uncertainty-about-a-novel-pandemic-health-threat-ambiguity-aversion-and-its-mechanisms
#19
Paul K J Han, Brian J Zikmund-Fisher, Christine W Duarte, Megan Knaus, Adam Black, Aaron M Scherer, Angela Fagerlin
Communicating scientific uncertainty about public health threats is ethically desirable but challenging due to its tendency to promote avoidance of choice options with unknown probabilities-a phenomenon known as "ambiguity aversion." This study examined this phenomenon's potential magnitude, its responses to different communication strategies, and its mechanisms. In a factorial experiment, 2701 adult laypersons in Spain read one of three versions of a hypothetical newspaper article describing a pandemic vaccine-preventable disease (VPD), but varying in scientific uncertainty about VPD risk and vaccine effectiveness: No-Uncertainty, Uncertainty, and Normalized-Uncertainty (emphasizing its expected nature)...
April 12, 2018: Journal of Health Communication
https://www.readbyqxmd.com/read/29648785/an-optical-technique-for-mapping-microviscosity-dynamics-in-cellular-organelles
#20
Joseph E Chambers, Marketa Kubankova, Roland G Huber, Ismeal López-Duarte, Edward Avezov, Peter J Bond, Stefan J Marciniak, Marina K Kuimova
Microscopic viscosity (microviscosity) is a key determinant of diffusion in the cell and defines the rate of biological processes occurring at the nanoscale, including enzyme-driven metabolism and protein folding. Here we establish a Rotor-based Organelle Viscosity Imaging (ROVI) methodology that enables real-time quantitative mapping of cell microviscosity. This approach uses environment sensitive dyes termed molecular rotors, covalently linked to genetically encoded probes to provide compartment specific microviscosity measurements via fluorescence lifetime imaging (FLIM)...
April 12, 2018: ACS Nano
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