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https://www.readbyqxmd.com/read/28812677/species-specific-responses-to-ocean-acidification-should-account-for-local-adaptation-and-adaptive-plasticity
#1
Cristian A Vargas, Nelson A Lagos, Marco A Lardies, Cristian Duarte, Patricio H Manríquez, Victor M Aguilera, Bernardo Broitman, Steve Widdicombe, Sam Dupont
Global stressors, such as ocean acidification, constitute a rapidly emerging and significant problem for marine organisms, ecosystem functioning and services. The coastal ecosystems of the Humboldt Current System (HCS) off Chile harbour a broad physical-chemical latitudinal and temporal gradient with considerable patchiness in local oceanographic conditions. This heterogeneity may, in turn, modulate the specific tolerances of organisms to climate stress in species with populations distributed along this environmental gradient...
March 13, 2017: Nature ecology & evolution
https://www.readbyqxmd.com/read/28811521/decadal-trends-in-red-sea-maximum-surface-temperature
#2
V Chaidez, D Dreano, S Agusti, C M Duarte, I Hoteit
Ocean warming is a major consequence of climate change, with the surface of the ocean having warmed by 0.11 °C decade(-1) over the last 50 years and is estimated to continue to warm by an additional 0.6 - 2.0 °C before the end of the century(1). However, there is considerable variability in the rates experienced by different ocean regions, so understanding regional trends is important to inform on possible stresses for marine organisms, particularly in warm seas where organisms may be already operating in the high end of their thermal tolerance...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809732/validation-of-the-simplified-criteria-for-the-diagnosis-of-autoimmune-hepatitis-in-chilean-hispanic-patients
#3
Roberto Candia, Blanca Norero, Carlos Agu Ero, Luis Díaz, Juan Pablo Ortega, Rodrigo Wolff, Cristian Hernández-Rocha, Ignacio Duarte, Alejandro Soza, Carlos Benítez, Marco Arrese
INTRODUCTION AND AIM: In 2008 the International autoimmune hepatitis (AIH) Group proposed the simplified diagnostic criteria for this disease. The original cohort study was performed in 11 international centers, but validation studies are scarce in Latin-America. The aim of this study is validate these criteria in Hispanic patients. MATERIAL AND METHODS: A retrospective cohort of patients undergoing percutaneous liver biopsy and follow-up of at least 12 months was recruited from a Chilean University hospital...
August 8, 2017: Annals of Hepatology
https://www.readbyqxmd.com/read/28806693/persistence-of-urban-organic-aerosols-composition-decoding-their-structural-complexity-and-seasonal-variability
#4
João T V Matos, Regina M B O Duarte, Sónia P Lopes, Artur M S Silva, Armando C Duarte
Organic Aerosols (OAs) are typically defined as highly complex matrices whose composition changes in time and space. Focusing on time vector, this work uses two-dimensional nuclear magnetic resonance (2D NMR) techniques to examine the structural features of water-soluble (WSOM) and alkaline-soluble organic matter (ASOM) sequentially extracted from fine atmospheric aerosols collected in an urban setting during cold and warm seasons. This study reveals molecular signatures not previously decoded in NMR-related studies of OAs as meaningful source markers...
August 11, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28803463/bacterial-microcolonies-in-gel-beads-for-high-throughput-screening-of-libraries-in-synthetic-biology
#5
José M Duarte, Içvara Barbier, Yolanda Schaerli
Synthetic biologists increasingly rely on directed evolution to optimize engineered biological systems. Applying an appropriate screening or selection method for identifying the potentially rare library members with the desired properties is a crucial step for success in these experiments. Special challenges include substantial cell-to-cell variability and the requirement to check multiple states (e.g. being ON or OFF depending on the input). Here, we present a high-throughput screening method that addresses these challenges...
August 14, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28801420/protocol-study-for-a-randomised-controlled-double-blind-clinical-trial-involving-virtual-reality-and-anodal-transcranial-direct-current-stimulation-for-the-improvement-of-upper-limb-motor-function-in-children-with-down-syndrome
#6
Jamile Benite Palma Lopes, Luanda André Collange Grecco, Renata Calhes Franco de Moura, Roberta Delasta Lazzari, Natalia de Almeida Carvalho Duarte, Isabela Miziara, Gileno Edu Lameira de Melo, Arislander Jonathan Lopes Dumont, Manuela Galli, Claudia Santos Oliveira
INTRODUCTION: Down syndrome results in neuromotor impairment that affects selective motor control, compromising the acquisition of motor skills and functional independence. The aim of the proposed study is to evaluate and compare the effects of multiple-monopolar anodal transcranial direct current stimulation and sham stimulation over the primary motor cortex during upper limb motor training involving virtual reality on motor control, muscle activity, cerebral activity and functional independence...
August 11, 2017: BMJ Open
https://www.readbyqxmd.com/read/28800953/col7a1-editing-via-crispr-cas9-in-recessive-dystrophic-epidermolysis-bullosa
#7
Stefan Hainzl, Patricia Peking, Thomas Kocher, Eva M Murauer, Fernando Larcher, Marcela Del Rio, Blanca Duarte, Markus Steiner, Alfred Klausegger, Johann W Bauer, Julia Reichelt, Ulrich Koller
Designer nucleases allow specific and precise genomic modifications and represent versatile molecular tools for the correction of disease-associated mutations. In this study, we have exploited an ex vivo CRISPR/Cas9-mediated homology-directed repair approach for the correction of a frequent inherited mutation in exon 80 of COL7A1, which impairs type VII collagen expression, causing the severe blistering skin disease recessive dystrophic epidermolysis bullosa. Upon CRISPR/Cas9 treatment of patient-derived keratinocytes, using either the wild-type Cas9 or D10A nickase, corrected single-cell clones expressed and secreted similar levels of type VII collagen as control keratinocytes...
July 13, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28800592/cx3cr1-knockout-aggravates-coxsackievirus-b3-induced-myocarditis
#8
Irene Müller, Kathleen Pappritz, Konstantinos Savvatis, Kerstin Puhl, Fengquan Dong, Muhammad El-Shafeey, Nazha Hamdani, Isabell Hamann, Michel Noutsias, Carmen Infante-Duarte, Wolfgang A Linke, Sophie Van Linthout, Carsten Tschöpe
Studies on inflammatory disorders elucidated the pivotal role of the CX3CL1/CX3CR1 axis with respect to the pathophysiology and diseases progression. Coxsackievirus B3 (CVB3)-induced myocarditis is associated with severe cardiac inflammation, which may progress to heart failure. We therefore investigated the influence of CX3CR1 ablation in the model of acute myocarditis, which was induced by inoculation with 5x105 plaque forming units of CVB3 (Nancy strain) in either CX3CR1-/- or C57BL6/j (WT) mice. Seven days after infection, myocardial inflammation, remodeling, and titin expression and phosphorylation were examined by immunohistochemistry, real-time PCR and Pro-Q diamond stain...
2017: PloS One
https://www.readbyqxmd.com/read/28800351/work-and-sustainable-development
#9
Pascal Béguin, Francisco Duarte
No abstract text is available yet for this article.
2017: Work: a Journal of Prevention, Assessment, and Rehabilitation
https://www.readbyqxmd.com/read/28800171/antioxidant-protection-by-%C3%AE-selenoamines-against-thioacetamide-induced-oxidative-stress-and-hepatotoxicity-in-mice
#10
Sílvio Terra Stefanello, Diane Duarte Hartmann, Guilherme Pires Amaral, Aline Alves Courtes, Martim T B Leite, Thayanara Cruz da Silva, Débora Farina Gonçalves, Micaela B Souza, Pâmela Carvalho da Rosa, Luciano Dornelles, Félix Alexandre Antunes Soares
Thioacetamide (TAA) is a hepatotoxin that rapidly triggers the necrotic process and oxidative stress in the liver. Nevertheless, organic selenium compounds, such as β-selenoamines, can be used as pharmacological agents to diminish the oxidative damage. Thus, the aim of this study was to investigate the protective effect of the antioxidant β-selenoamines on TAA-induced oxidative stress in mice. Here, we observed that a single intraperitoneal injection of TAA (200 mg/kg) dramatically elevated some parameters of oxidative stress, such as lipid peroxidation and reactive oxygen species (ROS) production, as well as depleted cellular antioxidant defenses...
August 11, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28798803/preparation-of-a-nanoemulsion-with-carapa-guianensis-aublet-meliaceae-oil-by-a-low-energy-solvent-free-method-and-evaluation-of-its-preliminary-residual-larvicidal-activity
#11
Flávia L M Jesus, Fernanda B de Almeida, Jonatas L Duarte, Anna E M F M Oliveira, Rodrigo A S Cruz, Raimundo N P Souto, Ricardo M A Ferreira, Regina Gendzelevski Kelmann, José C T Carvalho, Ana C Lira-Guedes, Marcelino Guedes, Conxita Solans, Caio P Fernandes
Andiroba (Carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. However, few studies have devoted attention to innovative formulations, including nanoemulsions. The present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. Moreover, the preliminary residual larvicidal activity of the nanoemulsion against Aedes aegypti was evaluated. Oleic and palmitic acids were the major fatty acids, in addition to the phytosterol β-sitosterol and limonoids (tetranortriterpenoids)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28798185/whole-genome-sequence-of-starmerella-bacillaris-pas13-a-nonconventional-enological-yeast-with-antifungal-activity
#12
Wilson José Fernandes Lemos Junior, Laura Treu, Vinícius da Silva Duarte, Milena Carlot, Chiara Nadai, Stefano Campanaro, Alessio Giacomini, Viviana Corich
Starmerella bacillaris is a fermentative yeast commonly found in vineyards. Here, we present the draft genome sequence of S. bacillaris PAS13, a nonconventional enological yeast with a potential role as a biocontrol agent. This gene sequence will provide insights into the genetic basis of yeast activity against gray mold disease (Botrytis cinerea).
August 10, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28797859/valuing-attributes-of-home-palliative-care-with-service-users-a-pilot-discrete-choice-experiment
#13
Barbara Gomes, Maja de Brito, Vera P Sarmento, Deokhee Yi, Duarte Soares, Jacinta Fernandes, Bruno Fonseca, Edna Gonçalves, Pedro L Ferreira, Irene J Higginson
CONTEXT: Discrete choice experiment (DCE) is a quantitative method that helps determine which service attributes are most valued by people and consequently improve their well-being. OBJECTIVES: To test a new DCE on home palliative care (HPC). METHODS: Cross-sectional survey using the DCE method with adult patients and their family caregivers, users of three HPC services in Portugal. Service attributes were based on a Cochrane review, a meta-ethnography, and the few existing DCEs on HPC: 1) team's availability, 2) support for family caregivers, 3) homecare support, 4) information and planning, and 5) waiting time...
August 7, 2017: Journal of Pain and Symptom Management
https://www.readbyqxmd.com/read/28795812/investigation-of-the-adsorption-of-amphipathic-macroraft-agents-onto-montmorillonite-clay
#14
Rodrigo Duarte Silva, Igor Stefanichen Monteiro, Thaïssa De Camargo Chaparro, Raíssa Silva Hardt, Reinaldo Giudici, Ana Barros-Timmons, Elodie Bourgeat-Lami, Amilton Martins Dos Santos
Recently, there has been significant interest in the use of the reversible addition-fragmentation chain-transfer (RAFT) technique to generate a variety of organic/inorganic colloidal composite particles in aqueous dispersed media using the so-called macroRAFT-assisted encapsulating emulsion polymerization (REEP) strategy. In this process, special attention should be paid to the adsorption of the macromolecular RAFT (macroRAFT) agent onto the inorganic particles as it determines the final particle morphology and can also influence latex stability...
August 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28793108/validation-of-educational-booklet-for-hiv-aids-prevention-in-older-adults
#15
Luana Ibiapina Cordeiro, Thais de Oliveira Lopes, Luciane Elise de Abreu Lira, Sarah Maria de Sousa Feitoza, Maria Eliana Peixoto Bessa, Maria Lúcia Duarte Pereira, Aline Rodrigues Feitoza, Adriano Rodrigues de Souza
Objective: To describe the process of manufacturing and validation of an educational booklet for HIV/Aids prevention in older adults. Methods: Methodological study developed in two phases - manufacturing of the booklet and validation of the educational material by judges. The manufacturing process involved a situational diagnosis with older adults, and its result indicated gaps in the knowledge with respect to HIV/Aids. The validation process was performed by nine judges, selected by convenience...
July 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/28793090/prevalence-of-erectile-dysfunction-oral-drugs-use-in-a-city-of-southern-brazil
#16
Daniel Vanti Duarte, María Clara Restrepo-Méndez, Mariângela Freitas da Silveira
The last decade has seen a breakthrough in the treatment of erectile dysfunction (ED) with the advent of phosphodiesterase-5 inhibitors. There are few population-based observational studies on the prevalence of use of these drugs. We conducted a cross-sectional population-based study in the city of Pelotas (Brazil). Our sample comprised 1,082 men aged 20 years or older who answered a confidential and self-administered questionnaire. Prevalence of EDD use was 5% (IC95% = 4%;7%). ED and advanced age were strongly associated with a higher prevalence of EDD use...
August 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28793089/-evolution-in-the-use-and-the-expenditures-of-a-healthcare-provider
#17
André Luís de Castro Moura Duarte, Felippe de Medeiros Oliveira, Anderson de Andrade Santos, Bento Fortunato Cardoso Dos Santos
Brazil's aging population and the rising number of people reliant upon the country's supplementary healthcare system have elicited the concern of public and private managers regarding the increase in healthcare costs. In this paper, the costs per gender, per type of medical expenses and per age group of a major Brazilian self-managed healthcare provider between 2007 and 2013 were analyzed. This healthcare provider is of interest because, besides portraying a single condition of revenue growth restricted to the existing contributors, it also replicates the demographic profile expected for Brazil in 2050, when approximately one-third of its population will be over 60 years of age...
August 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28793003/complete-axillary-dissection-without-drainage-for-the-surgical-treatment-of-breast-cancer-a-randomized-clinical-trial
#18
Ruffo Freitas-Junior, Luís Fernando Jubé Ribeiro, Marise Amaral Rebouças Moreira, Geraldo Silva Queiroz, Maurício Duarte Esperidião, Marco Aurélio Costa Silva, Rubens José Pereira, Rossana Araújo Catão Zampronha, Rosemar Macedo Sousa Rahal, Leonardo Ribeiro Soares, Danielle Laperche Dos Santos, Maria Virginia Thomazini, Cassiana Ferreira Silva de Faria, Régis Resende Paulinelli
OBJECTIVE: This randomized clinical trial evaluated the possibility of not draining the axilla following axillary dissection. METHODS: The study included 240 breast cancer patients who underwent axillary dissection as part of conservative treatment. The patients were divided into two groups depending on whether or not they were subjected to axillary drainage. ClinicalTrials.gov: NCT01267552. RESULTS: The median volume of fluid aspirated was significantly lower in the axillary drainage group (0...
July 2017: Clinics
https://www.readbyqxmd.com/read/28792614/mutation-rates-in-21-autosomal-short-tandem-repeat-loci-in-a-population-from-goi%C3%A3-s-brazil
#19
Thaís Cidália Vieira, Marc Alexandre Duarte Gigonzac, Ricardo Goulart Rodovalho, Luana Morais Cavalcanti, Lysa Bernardes Minasi, Flávia Melo Rodrigues, Aparecido Divino da Cruz
The appearance of new mutations in polymorphic markers plays a central role in a range of genetic applications, including dating phylogenetic events, informing disease studies, and evaluating forensic evidence. The present study estimated the mutation rates of 21 autosomal short tandem repeat (STR) loci in a population from Central Brazil. We studied 15,046 paternity cases from Goiás, Brazil from August 2012 to February 2015. We identified 262 mutations in the 21 loci. The loci that presented more mutations were FGA and D18S51, with a total of 46 and 28 mutations, respectively...
August 9, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28789851/antibody-persistence-and-evidence-of-immune-memory-at-5years-following-administration-of-the-9-valent-hpv-vaccine
#20
Ana Guevara, Robinson Cabello, Linn Woelber, Edson Duarte Moreira, Elmar Joura, Olaf Reich, Christine Shields, Misoo C Ellison, Amita Joshi, Alain Luxembourg
BACKGROUND: The 9-valent HPV (9vHPV) vaccine was developed to prevent infection and disease related to 9 HPV types (HPV6/11/16/18/31/33/45/52/58) which cause approximately 90% of cervical cancers, HPV-related vulvar, vaginal and anal cancers, and genital warts worldwide. In a pivotal efficacy study, the 9vHPV vaccine prevented infection and disease due to the 9 vaccine types. Duration of protection remains to be determined. Vaccines that induce long-term protection are generally characterized by the generation of immune memory...
August 5, 2017: Vaccine
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