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Fanghao Hu, Chen Zeng, Rong Long, Yupeng Miao, Lu Wei, Qizhi Xu, Wei Min
Optical multiplexing has a large impact in photonics, the life sciences and biomedicine. However, current technology is limited by a 'multiplexing ceiling' from existing optical materials. Here we engineered a class of polyyne-based materials for optical supermultiplexing. We achieved 20 distinct Raman frequencies, as 'Carbon rainbow', through rational engineering of conjugation length, bond-selective isotope doping and end-capping substitution of polyynes. With further probe functionalization, we demonstrated ten-color organelle imaging in individual living cells with high specificity, sensitivity and photostability...
January 15, 2018: Nature Methods
S Vijayakumar, P Krishnakumar, P Arulmozhi, S Mahadevan, N Parameaswari
Biosynthesized nanoparticles have an incredible application in biomedicine owing to its simplicity, eco-friendly properties and low cost. The present study aims to determine the green synthesized zinc oxide nanoparticles from methanolic leaf extract of Glycosmis pentaphylla. The synthesized nanoparticles were characterized using UV-VIS Spectroscopy, Flourescence spectrometer, FT-IR, XRD, SEM with EDAX and TEM. The confirmations of synthesized nanoparticles were characterized by peak at 351 and 410 nm in the UV-VIS spectrum and photoluminescence spectrum respectively...
January 9, 2018: Microbial Pathogenesis
Hongjian He, Jiaqing Wang, Huaimin Wang, Ning Zhou, Dongsik Yang, Douglas R Green, Bing Xu
Most of the reported mitochondria targeting molecules are lipophilic and cationic, which may become cytotoxic with accumulation. Here we show enzymatic cleavage of branched peptides that carry negative charges for targeting mitochondria. Conjugating a well-established protein tag (i.e., FLAG-tag) to self-assembling motifs affords the pre-cursors that form micelles. Enzymatic cleavage of the hydrophilic FLAG motif (DDDDK) by enterokinase (ENTK) turns the micelles to nanofibers. After being taken up by cells, the micelles, upon the action of intracellular ENTK, turns into nanofibers to locate mainly at mitochondria...
January 12, 2018: Journal of the American Chemical Society
Pooyath Lekshmy Venugopalan, Shilpee Jain, Srinivasrao Shivashankar, Ambarish Ghosh
Magnetic nanomotors with integrated theranostic capabilities can revolutionize biomedicine of the future. Typically, these nanomotors contain ferromagnetic materials, such that small magnetic fields can be used to maneuver and localize them in fluidic or gel-like environments. Motors with large permanent magnetic moments tend to agglomerate, which limits the scalability of this otherwise promising technology. Here, we demonstrate the application of a microwave-synthesized ferrite layer to reduce the agglomeration of helical ferromagnetic nanomotors by an order of magnitude, which allows them to be stored in a colloidal suspension for longer than six months and subsequently be manoeuvred with undiminished performance...
January 12, 2018: Nanoscale
Songfeng Pei, Qinwei Wei, Kun Huang, Hui-Ming Cheng, Wencai Ren
Graphene oxide is highly desired for printing electronics, catalysis, energy storage, separation membranes, biomedicine, and composites. However, the present synthesis methods depend on the reactions of graphite with mixed strong oxidants, which suffer from explosion risk, serious environmental pollution, and long-reaction time up to hundreds of hours. Here, we report a scalable, safe and green method to synthesize graphene oxide with a high yield based on water electrolytic oxidation of graphite. The graphite lattice is fully oxidized within a few seconds in our electrochemical oxidation reaction, and the graphene oxide obtained is similar to those achieved by the present methods...
January 10, 2018: Nature Communications
Hittu Matta, Ramakrishnan Gopalakrishnan, Sunju Choi, Rekha Prakash, Venkatesh Natarajan, Ruben Prins, Songjie Gong, Saurabh D Chitnis, Michael Kahn, Xu Han, Vishan Chaudhary, Adam Soni, Jennifer Sernas, Prottasha Khan, Dan Wang, Preet M Chaudhary
A simple, accurate, sensitive and robust assay that can rapidly and specifically measure the death of target cells would have applications in many areas of biomedicine and particularly for the development of novel cellular- and immune-therapeutics. In this study, we describe a novel cytotoxicity assay, termed the Matador assay, which takes advantage of the extreme brightness, stability and glow-like characteristics of recently discovered novel marine luciferases and their engineered derivatives. The assay involves expression of a luciferase of interest in target cells in a manner so that it is preferentially retained within the healthy cells but is either released from dead and dying cells or whose activity can be preferentially measured in dead and dying cells...
January 9, 2018: Scientific Reports
Qianqian Kang, Zhaolin Sun, Zhiyuan Zou, Ming Wang, Qiuyan Li, Xiaoxiang Hu, Ning Li
Cell-penetrating peptides (CPPs) have been increasingly used to deliver various molecules, both in vitro and in vivo. However, there are no reports of CPPs being used in porcine fetal fibroblasts (PFFs). The increased use of transgenic pigs for basic research and biomedical applications depends on the availability of technologies for efficient genetic-modification of PFFs. Here, we report that three CPPs (CPP5, TAT, and R9) can efficiently deliver active Cre recombinase protein into PFFs via an energy-dependent endocytosis pathway...
2018: PloS One
Jingjing Gao, Xioachi Liu, Hatice Secinti, Ziwen Jiang, Oyuntuya Munkhbat, Yisheng Xu, Xuhong Guo, Sankaran Thayumanavan
Specific response to the concurrent presence of two different inputs is one of the hallmarks of incorporating specificities in Nature. Artificial nanoassemblies that similarly respond to two very different inputs are of great interest in a variety of applications, especially in biomedicine. Here, we present a design strategy for amphiphilic nanoassemblies with such capabilities, enabled by photocaging a ligand moiety that is capable of binding to a specific protein. New molecular designs that offer nanoassemblies that respond to either of two inputs or only to the concurrent presence of two inputs are outlined...
January 3, 2018: Chemistry: a European Journal
Hongfei He, Jianyuan Dai, Yan Meng, Zhijuan Duan, Cuisong Zhou, Baozhan Zheng, Juan Du, Yong Guo, Dan Xiao
It is known that DNA molecules can be used to build a various of complicated geometrical DNA nanostructures with programmable sequence design, and these DNA nanomaterials show a promising application in biotechnology and biomedicine. However, the construction of large-sized three dimensional DNA-based nanomaterials still remains a challenge. In this work, we propose a new strategy that only employs one target DNA to trigger multiple catalyzed hairpin assembly (CHA) reactions and sticky ends self-assembly to prepare hundreds of nanometer-sized DNA nanoparticles...
March 1, 2018: Talanta
Peter Henningsen, Stephan Zipfel, Heribert Sattel, Francis Creed
Functional somatic syndromes (FSS), like irritable bowel syndrome or fibromyalgia and other symptoms reflecting bodily distress, are common in practically all areas of medicine worldwide. Diagnostic and therapeutic approaches to these symptoms and syndromes vary substantially across and within medical specialties from biomedicine to psychiatry. Patients may become frustrated with the lack of effective treatment, doctors may experience these disorders as difficult to treat, and this type of health problem forms an important component of the global burden of disease...
January 6, 2018: Psychotherapy and Psychosomatics
M C R Miranda, F A Borges, N R Barros, N A Santos Filho, R J Mendonça, R D Herculano, E M Cilli
The biomembrane natural (NRL-Natural Rubber Latex), manipulated from the latex obtained from the rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Reflecting the biocompatibility and low bounce rate of this material, NRL has been used as a physical barrier to infectious agents and for the controlled release of drugs and extracts. The aim of the present study was to evaluate the incorporation and release of peptides using a latex biomembrane carrier. After incorporation, the release of material from the membrane was observed using spectrophotometry...
January 5, 2018: Amino Acids
Pilar Rivera Gil, Dionysia Tsouts, Can Xiao, Weiteng An, Marcos Sanles-Sobrido, Andreu Cabo
In this review, we introduce the reader the analytical technique, surface-enhanced Raman scattering motivated by the great potential we believe this technique have in biomedicine. We present the advantages and limitations of this technique relevant for bioanalysis in vitro and in vivo and how this technique goes beyond the state of the art of traditional analytical, labelling and healthcare diagnosis technologies.
January 4, 2018: Current Medicinal Chemistry
Jing Chen, Ke Huang, Qijun Chen, Chao Deng, Jian Zhang, Zhiyuan Zhong
Antibody therapeutics though representing a most used biomedicine suffers from poor in vivo stability, rapid degradation, and frequent injections. Here, we report that fluorescent hyaluronic acid microgels (HMGs) tailor-made by combining microfluidics and "tetrazole-alkene" photoclick chemistry enable sustained and localized delivery of Herceptin in ovarian tumor. HMGs were obtained with defined size (25-50 μm), narrow size distribution, high stability, and strong green fluorescence. Notably, HMGs exhibited a remarkably high loading of proteins like Herceptin and IgG with a loading efficiency exceeding 90% at a theoretical protein loading content of 30 wt...
January 5, 2018: ACS Applied Materials & Interfaces
Peige Song, Jiawen Wang, Kajo Bucan, Evropi Theodoratou, Igor Rudan, Kit Yee Chan
Background: Glaucoma, the second leading cause of blindness, affects approximately 64.3 million individuals worldwide. In China, demographic ageing is in rapid progress. Yet detailed and up-to-date estimates of the scale of glaucoma are rare. We aimed to quantify and understand the prevalence and burden of glaucoma in China from 1990 to 2015, with projections until 2050. Methods: For this systematic review and meta-analysis, we searched China National Knowledge Infrastructure (CNKI), Wanfang, Chinese Biomedicine Literature Database (CBM-SinoMed), PubMed, Embase and Medline using comprehensive search strategies to identify all relevant articles that have reported the prevalence of glaucoma in the general Chinese population...
December 2017: Journal of Global Health
Peige Song, Yuhang Du, Kit Yee Chan, Evropi Theodoratou, Igor Rudan
Background: Age-related macular degeneration (AMD) is the third most common cause of blindness, and the fourth leading cause of visual impairment worldwide, but little is known about the burden of this disease in the most populous country-China. This study provides the first comprehensive estimates of the prevalence and burden of AMD in China from 1990 to 2015, with projections till 2050. Methods: In this study, a systematic review and meta-analysis was conducted to estimate the prevalence of AMD in China...
December 2017: Journal of Global Health
Vidushi Neergheen-Bhujun, Almas Taj Awan, Yusuf Baran, Nils Bunnefeld, Kit Chan, Thomas Edison Dela Cruz, Dilfuza Egamberdieva, Simon Elsässer, Mari-Vaughn V Johnson, Shoji Komai, Andrey L Konevega, John H Malone, Paul Mason, Rothsophal Nguon, Ross Piper, Uttam Babu Shrestha, Milica Pešić, Alexander Kagansky
No abstract text is available yet for this article.
December 2017: Journal of Global Health
Kira Astakova, Roslyn Ray, Maria Taskova, Jesper Uhd, Annika Carstens, Kevin Morris
The use of nucleic acid, DNA and RNA, based strategies to disrupt gene expression as a therapeutic is quickly emerging. Indeed, synthetic oligonucleotides represent a major component of emerging gene therapeutics. However, the efficiency and specificity of intracellular uptake for non-modified oligonucleotides is rather poor. Utilizing RNA based oligonucleotides as therapeutics is even more challenging to deliver, due to extremely fast enzymatic degradation of the RNAs. Similar to unmodified oligonucleotides, RNAs also get rapidly degraded in vivo and show large off-target binding even when they are able to reach and enter the desired target cells...
January 4, 2018: Molecular Pharmaceutics
Qi Zhang, Shiyu Lin, Sirong Shi, Tao Zhang, Quanquan Ma, Taoran Tian, Tengfei Zhou, Xiaoxiao Cai, Yunfeng Lin
Tetrahedral DNA nanostructures (TDNs) are a new type of nano-materials that have recently attracted attention in the field of biomedicine. However, the practical application of nanomaterials is often limited owing to the host immune response. Here, the response of RAW264.7 macrophages to TDNs was comprehensively evaluated. The results showed that TDNs had no observable cytotoxicity and could induce polarization of RAW264.7 cells to M1 type. TDNs attenuated the expression of NO IL-1β (Interleukin-1β), IL-6 (interleukin-6), and TNF-α (tumor necrosis factor-α) in LPS-induced RAW264...
January 4, 2018: ACS Applied Materials & Interfaces
Chih-Hsiang Huang, Yu-Wen Chen, Chih-Ming Chen
Titanium (Ti) has high potential in many practical applications such as biomedicine, architecture, aviation, and energy. In this study, we demonstrate an innovative application of dye-sensitized solar cell (DSSC) based on Ti photoanode that can be integrated into the roof engineering of large-scale architectures. A chromatic Ti foil produced by anodizing oxidation (coloring) technology (AOT) is an attractive roof material for large-scale architecture, showing a colorful appearance due to the formation of a reflective TiO2 thin layer on both surfaces of Ti...
January 4, 2018: ACS Applied Materials & Interfaces
Hanan Karimah Kiranda, Rozi Mahmud, Danmaigoro Abubakar, Zuki Abubakar Zakaria
The evolution of nanomaterial in science has brought about a growing increase in nanotechnology, biomedicine, and engineering fields. This study was aimed at fabrication and characterization of conjugated gold-cockle shell-derived calcium carbonate nanoparticles (Au-CSCaCO3NPs) for biomedical application. The synthetic technique employed used gold nanoparticle citrate reduction method and a simple precipitation method coupled with mechanical use of a Programmable roller-ball mill. The synthesized conjugated nanomaterial was characterized for its physicochemical properties using transmission electron microscope (TEM), field emission scanning electron microscope (FESEM) equipped with energy dispersive X-ray (EDX) and Fourier transform infrared spectroscopy (FTIR)...
January 2, 2018: Nanoscale Research Letters
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