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https://www.readbyqxmd.com/read/29777551/a-new-method-for-quantitative-determination-of-renalase-based-on-mass-spectrometry-determination-of-a-proteotypic-peptide-labeled-with-stable-isotopes
#1
A T Kopylov, V I Fedchenko, O A Buneeva, N V Pyatakova, V G Zgoda, A E Medvedev
RATIONALE: Renalase is a recently discovered kidney secretory protein, which is considered as an important component involved in blood pressure regulation. Although altered levels of renalase have been detected in plasma and urine of patients with various kidney diseases, there is certain inconsistency of changes in the renalase levels reported by different laboratories. The latter is obviously associated with the use of ELISA as the only available approach for quantitative analysis of renalase...
May 18, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29756649/measuring-peripheral-oxytocin-and-vasopressin-in-nonhuman-primates
#2
REVIEW
Toni E Ziegler
Studying the neural and hormonal changes that modulate behavior is critical to understanding social relationships. Of particular interest is measuring oxytocin (OT) and arginine vasopressin (AVP) peripherally, and preferably, non-invasively, in nonhuman primates. Due to these peptides' neural origin and their stimulation of brain areas that influence social behavior, there has been debate whether peripheral measures in blood, urine, and saliva reflect central levels in the brain. This review elucidates the challenges of OT measurement and the solutions that provide valuable data on OT's role in social behavior...
May 14, 2018: American Journal of Primatology
https://www.readbyqxmd.com/read/29755469/urinary-peptides-as-a-novel-source-of-t-cell-allergen-epitopes
#3
Ricardo da Silva Antunes, John Pham, Curtis McMurtrey, William H Hildebrand, Elizabeth Phillips, Simon Mallal, John Sidney, Paula Busse, Bjoern Peters, Véronique Schulten, Alessandro Sette
Mouse allergy in both laboratory workers and in inner-city children is associated with allergic rhinitis and asthma, posing a serious public health concern. Urine is a major source of mouse allergens, as mice spray urine onto their surroundings, where the proteins dry up and become airborne on dust particles. Here, we tested whether oligopeptides that are abundant in mouse urine may contribute to mouse allergic T cell response. Over 1,300 distinct oligopeptides were detected by mass spectrometry analysis of the low molecular weight filtrate fraction of mouse urine (LoMo)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29753374/beneficial-effects-of-pentoxifylline-plus-losartan-dual-therapy-in-type-2-diabetes-with-nephropathy
#4
Soghra Rabizadeh, Fatemeh Dehghani Firouzabadi, Sina Noshad, Sadaf Esteghamati, Mohsen Afarideh, Alireza Ghajar, Morsaleh Ganji, Mohammad Saadat, Behnam Heidari, Mohammad Taghi Najafi, Manouchehr Nakhjavani, Alireza Esteghamati
BACKGROUND: This study was designed to comparatively assess the effects of add-on pentoxifylline to losartan versus increasing the dose of losartan on serum N-terminal pro-brain natriuretic peptide (NT-proBNP), serum highly sensitive C-reactive protein (hsCRP) and the urinary albumin excretion (UAE) rate in patients with type 2 diabetes and nephropathy. METHODS: In an open-label, single-center, parallel-group, randomized clinical trial (NCT03006952), 30 patients received b...
May 2018: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29752915/increase-in-insulin-secretion-and-decrease-in-muscle-degradation-by-fat-free-milk-intake-are-attenuated-by-physical-exercise
#5
Tetsuo Yamada, Masami Matsuzaki, Akira Tanaka
BACKGROUND: Protein intake, particularly branched chain amino acids (BCAAs), and exercise have opposing actions on insulin secretion, but the same action on protein anabolism. We examined the effects of BCAA-rich fat-free milk intake and/or exercise on levels of insulin secretion and indices related to muscle protein metabolism in order to assess the potency of dietary and exercise therapies against metabolic and locomotive disorders. METHODS: Eight adult female volunteers participated in all four 24 h experiments; control diet intake with or without exercise, and fat-free milk-containing diet intake with or without exercise...
May 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29734386/renal-papillary-tip-extract-stimulates-bnp-production-and-excretion-from-cardiomyocytes
#6
Itaru Goto, Ryuji Okamoto, Ryotaro Hashizume, Noboru Suzuki, Rie Ito, Keiichi Yamanaka, Hiromitsu Saito, Hiroshi Kiyonari, Isao Tawara, Yuki Kageyama, Yoshito Ogihara, Yusuf Ali, Norikazu Yamada, Naoyuki Katayama, Masaaki Ito
BACKGROUND: Brain natriuretic peptide (BNP) is an important biomarker for patients with cardiovascular diseases, including heart failure, hypertension and cardiac hypertrophy. It is also known that BNP levels are relatively higher in patients with chronic kidney disease and no heart disease; however, the mechanism remains unclear. METHODS AND RESULTS: We developed a BNP reporter mouse and occasionally found that this promoter was activated specifically in the papillary tip of the kidneys, and its activation was not accompanied by BNP mRNA expression...
2018: PloS One
https://www.readbyqxmd.com/read/29730764/status-report-on-the-high-throughput-characterization-of-complex-intact-o-glycopeptide-mixtures
#7
Adam Pap, Eva Klement, Eva Hunyadi-Gulyas, Zsuzsanna Darula, Katalin F Medzihradszky
A very complex mixture of intact, human N- and O-glycopeptides, enriched from the tryptic digest of urinary proteins of three healthy donors using a two-step lectin affinity enrichment, was analyzed by LC-MS/MS, leading to approximately 45,000 glycopeptide EThcD spectra. Two search engines, Byonic and Protein Prospector, were used for the interpretation of the data, and N- and O-linked glycopeptides were assigned from separate searches. The identification rate was very low in all searches, even when results were combined...
May 5, 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29729375/clinical-predictive-biomarkers-for-normoalbuminuric-diabetic-kidney-disease
#8
Tomohito Gohda, Yuji Nishizaki, Maki Murakoshi, Shuko Nojiri, Naotake Yanagisawa, Terumi Shibata, Mami Yamashita, Kanako Tanaka, Yoshinori Yamashita, Yusuke Suzuki, Nozomu Kamei
AIMS: A portion of patients with diabetes mellitus follow the progression of a non-albuminuria-based pathway; i.e., normoalbuminuric diabetic kidney disease (NA-DKD). However, the risk factors which determine NA-DKD are not yet fully understood. This cross-sectional study was therefore aimed to investigate the association between various biomarker levels and estimated glomerular filtration rate (eGFR) in patients with type 2 diabetes mellitus and normoalbuminuria (T2D-NA). METHODS: We measured cardiovascular disease (CVD) [serum osteoprotegerin (OPG), plasma brain natriuretic peptide (BNP), cardio-ankle vascular index (CAVI)], tubular damage [urinary L-type fatty acid binding protein (L-FABP)], and inflammatory [serum tumornecrosis factor (TNF) α and its receptors (TNFRs)] biomarkers in 314 patients with T2D-NA...
May 3, 2018: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/29724737/insulin-sensitivity-assessed-using-urine-c-peptide-creatinine-ratio-ucpcr-in-pregnancy-cross-sectional-analysis-of-an-english-multiethnic-cohort
#9
Ankica Markoska, Lilian C Mendoza, Rajalakshmi Valaiyapathi, Chloe Thorn, Anne Dornhorst
AIMS: To assess urinary C peptide creatinine ratio (UCPCR) used in a modified Matsuda equation to measure insulin sensitivity (IS) in pregnancy. RESEARCH AND DESIGN METHODS: In this cross-sectional study, two IS measurements were calculated in 73 pregnant women at ~28 weeks of gestation by two separate methods using modified Matsuda equations. The first using the 0 and 120 min serum C peptide concentration during a 75 g oral glucose tolerance test (OGTT) and the second using the 0 and 120 min UCPCR values...
May 3, 2018: BMJ Open
https://www.readbyqxmd.com/read/29706289/urinary-antimicrobial-peptides-potential-novel-biomarkers-of-obstructive-uropathy
#10
S Gupta, A R Jackson, D G DaJusta, D J McLeod, S A Alpert, V R Jayanthi, K McHugh, A R Schwaderer, B Becknell, C B Ching
INTRODUCTION: Antimicrobial peptides (AMPs) have historically been evaluated for their role in protecting against uropathogens. However, there is mounting evidence to support their expression in noninfectious injury, with unclear meaning as to their function. It is possible that AMPs represent urothelial injury. Urinary tract obstruction is known to alter the urothelium; however, AMPs have not been evaluated for expression in this noninfectious injury. OBJECTIVE: A pilot study to compare urinary AMP expression in children undergoing surgical intervention for ureteropelvic junction obstruction (UPJO) with nonobstructed controls...
March 29, 2018: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/29705701/protective-role-of-agrp-neuron-s-pdk1-against-salt-induced-hypertension
#11
Boyang Zhang, Masanori Nakata, Ming Lu, Jun Nakae, Takashi Okada, Wataru Ogawa, Toshihiko Yada
In the hypothalamic arcuate nucleus (ARC), orexigenic agouti-related peptide (AgRP) neurons regulate feeding behavior and energy homeostasis. The 3-phosphoinositide-dependent protein kinase-1 (PDK1) in AgRP neurons serves as a major signaling molecule for leptin and insulin, the hormones regulating feeding behavior, energy homeostasis and circulation. However, it is unclear whether PDK1 in AGRP neurons is also involved in regulation of blood pressure. This study explored it by generating and analyzing AgRP neuron-specific PDK1 knockout (Agrp-Pdk1flox/flox ) mice and effect of high salt diet on blood pressure in KO and WT mice was analyzed...
April 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29697035/serum-n-terminal-pro-brain-natriuretic-peptide-level-is-associated-with-the-development-of-chronic-kidney-diseases-in-patients-with-type-2-diabetes
#12
Ryo Sawada, Yoshitaka Hashimoto, Takafumi Senmaru, Muhei Tanaka, Emi Ushigome, Masahiro Yamazaki, Michiaki Fukui
BACKGROUND AND OBJECTIVE: Previous studies revealed the association between serum N-terminal pro-brain natriuretic peptide (NT-proBNP) level and chronic kidney diseases (CKD) in general population. However, little is known about the association between serum NT-proBNP level and incident CKD in patients with type 2 diabetes. Thus, we investigated the impact of serum NT-proBNP level on incident CKD in patients with type 2 diabetes. METHOD: We enrolled 211 type 2 diabetic patients without CKD in this cohort study...
April 25, 2018: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/29681522/epidemiologic-observations-guiding-clinical-application-of-a-urinary-peptidomic-marker-of-diastolic-left-ventricular-dysfunction
#13
Zhen-Yu Zhang, Esther Nkuipou-Kenfack, Wen-Yi Yang, Fang-Fei Wei, Nicholas Cauwenberghs, Lutgarde Thijs, Qi-Fang Huang, Ying-Mei Feng, Joost P Schanstra, Tatiana Kuznetsova, Jens-Uwe Voigt, Peter Verhamme, Harald Mischak, Jan A Staessen
Hypertension, obesity, and old age are major risk factors for left ventricular (LV) diastolic dysfunction (LVDD), but easily applicable screening tools for people at risk are lacking. We investigated whether HF1, a urinary biomarker consisting of 85 peptides, can predict over a 5-year time span mildly impaired diastolic LV function as assessed by echocardiography. In 645 white Flemish (50.5% women; 50.9 years [mean]), we measured HF1 by capillary electrophoresis coupled with mass spectrometry in 2005-2010. We measured early (E) and late (A) peak velocities of the transmitral blood flow and early (e') and late (a') mitral annular peak velocities and their ratios in 2009-2013...
March 21, 2018: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/29672270/role-of-atrial-natriuretic-peptide-in-controlling-diabetic-nephropathy-in-rats
#14
Lakhwinder Singh, Atul Arya, Sumeet Gupta
BACKGROUND: Diabetes is a downregulator of atrial natriuretic peptide (ANP), resulting in reduced nitric oxide level and low expression of endothelial nitric oxide synthase by which nitric oxide level get reduced. In the present study, we examined the role of ANP in reduced nitric oxide level, which may be responsible in controlling diabetic nephropathy in rats. METHODS: Serum nitrite/nitrate ratio, blood urea nitrogen, protein in urine, urinary output, serum creatinine, serum cholesterol, kidney weight, kidney hypertrophy, renal cortical collagen content, thiobarbituric acid level, and antioxidant enzymatic activities were assessed...
April 19, 2018: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/29665026/anhydrous-polymer-based-coating-with-sustainable-controlled-release-functionality-for-facile-efficacious-impregnation-and-delivery-of-antimicrobial-peptides
#15
Kaiyang Lim, Rathi Saravanan, Kian Long Kelvin Chong, Hwee Mian Sharon Goh, Ray Rong Yuan Chua, Paul Anantharajah Tambyah, Matthew Wook Chang, Kimberly A Kline, Susanna Su Jan Leong
Anhydrous polymers are actively explored as alternative materials to overcome limitations of conventional hydrogel-based antibacterial coating. However, the requirement for strong organic solvent in polymerization reactions often necessitates extra protection steps for encapsulation of target biomolecules, lowering encapsulation efficiency and increasing process complexity. This study reports a novel coating strategy that allows direct solvation and encapsulation of antimicrobial peptides (HHC36) into anhydrous polycaprolactone (PCL) polymer-based dual layer coating...
April 17, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29652493/reprogramming-probiotic-lactobacillus-reuteri-as-a-biosensor-for-staphylococcus-aureus-derived-aip-i-detection
#16
David Lubkowicz, Chun Loong Ho, In Young Hwang, Wen Shan Yew, Yung Seng Lee, Matthew Wook Chang
Gram-positive Staphylococcus aureus infection that results in pneumonia, urinary tract infection, and in severe cases, sepsis, has recently been classified as a serious threat to public health. Rapid and cost-effective detection of these infections are costly and time-consuming. Here, we present probiotic lactic acid bacteria engineered to detect autoinducer peptide-I (AIP-I), a quorum sensing molecule produced by Staphylococcus sp. during pathogenesis. We achieved this by adapting the well-characterized agr quorum sensing ( agrQS) from Staphylococcus aureus into Lactobacillus reuteri...
April 13, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29627295/deficiency-of-aldose-reductase-exacerbates-early-pressure-overload-induced-cardiac-dysfunction-and-autophagy-in-mice
#17
Shahid P Baba, Deqing Zhang, Mahavir Singh, Sujith Dassanayaka, Zhengzhi Xie, Ganapathy Jagatheesan, Jingjing Zhao, Virginia K Schmidtke, Kenneth R Brittian, Michael L Merchant, Daniel J Conklin, Steven P Jones, Aruni Bhatnagar
Pathological cardiac hypertrophy is associated with the accumulation of lipid peroxidation-derived aldehydes such as 4-hydroxy-trans-2-nonenal (HNE) and acrolein in the heart. These aldehydes are metabolized via several pathways, of which aldose reductase (AR) represents a broad-specificity route for their elimination. We tested the hypothesis that by preventing aldehyde removal, AR deficiency accentuates the pathological effects of transverse aortic constriction (TAC). We found that the levels of AR in the heart were increased in mice subjected to TAC for 2 weeks...
April 5, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29625564/urinary-proteomics-for-prediction-of-mortality-in-patients-with-type-2-diabetes-and-microalbuminuria
#18
Gemma E Currie, Bernt Johan von Scholten, Sheon Mary, Jose-Luis Flores Guerrero, Morten Lindhardt, Henrik Reinhard, Peter K Jacobsen, William Mullen, Hans-Henrik Parving, Harald Mischak, Peter Rossing, Christian Delles
BACKGROUND: The urinary proteomic classifier CKD273 has shown promise for prediction of progressive diabetic nephropathy (DN). Whether it is also a determinant of mortality and cardiovascular disease in patients with microalbuminuria (MA) is unknown. METHODS: Urine samples were obtained from 155 patients with type 2 diabetes and confirmed microalbuminuria. Proteomic analysis was undertaken using capillary electrophoresis coupled to mass spectrometry to determine the CKD273 classifier score...
April 6, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29617052/retinopathy-of-prematurity-screening-at-%C3%A2-30-weeks-urinary-ntpro-bnp-performance
#19
J E Berrington, P Clarke, N D Embleton, A K Ewer, R Geethanath, S Gupta, M Lal, S Oddie, A Shafiq, C Vasudevan, C Bührer
AIM: Urinary N-terminal B-type natriuretic peptide NTproBNP levels are associated with the development of retinopathy of prematurity (ROP) in infants <30 weeks gestation. The incidence of ROP in more mature infants who meet other ROP screening criteria is very low. We therefore aimed to test whether urinary NTproBNP predicted ROP development in these infants. METHODS: Prospective observational study in 151 UK infants ≥30+0 weeks gestation but also <32 weeks gestation and/or <1501g, to test the hypothesis that urinary NTproBNP levels on day of life (DOL) 14 and 28 were able to predict ROP development...
April 4, 2018: Acta Paediatrica
https://www.readbyqxmd.com/read/29611317/comparison-of-urine-and-plasma-peptidome-indicates-selectivity-in-renal-peptide-handling
#20
Pedro Magalhães, Claudia Pontillo, Martin Pejchinovski, Justyna Siwy, Magdalena Krochmal, Manousos Makridakis, Emma Carrick, Julie Klein, William Mullen, Joachim Jankowski, Antonia Vlahou, Harald Mischak, Joost P Schanstra, Petra Zürbig, Lars Pape
PURPOSE: Urine is considered to be produced predominantly as a result of plasma filtration in the kidney. However, the origin of the native peptides present in urine has never been investigated in detail. Therefore, we aimed to obtain a first insight into the origin of urinary peptides based on a side-by-side comprehensive analysis of the plasma and urine peptidome. METHODS: Twenty-two matched urine and plasma samples were analyzed for their peptidome using capillary electrophoresis coupled to mass spectrometry (CE-MS; for relative quantification) and CE- or LC coupled to tandem mass spectrometry (CE- or LC- MS/MS; for peptide identification)...
April 3, 2018: Proteomics. Clinical Applications
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