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urinary peptides

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https://www.readbyqxmd.com/read/28717416/feasibility-of-urinary-extracellular-vesicle-proteome-profiling-using-a-robust-and-simple-clinically-applicable-isolation-method
#1
Irene V Bijnsdorp, Olga Maxouri, Aarzo Kardar, Tim Schelfhorst, Sander R Piersma, Thang V Pham, Andre Vis, R Jeroen van Moorselaar, Connie R Jimenez
Extracellular vesicles (EVs) secreted by prostate cancer (PCa) cells contain specific biomarkers and can be isolated from urine. Collection of urine is not invasive, and therefore urinary EVs represent a liquid biopsy for diagnostic and prognostic testing for PCa. In this study, we optimised urinary EV isolation using a method based on heat shock proteins and compared it to gold-standard ultracentrifugation. The urinary EV isolation protocol using the Vn96-peptide is easier, time convenient (≈1.5 h) and no special equipment is needed, in contrast to ultracentrifugation protocol (>3...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28704490/the-synthetic-killer-peptide-kp-impairs-candida-albicans-biofilm-in-vitro
#2
Simona Paulone, Andrea Ardizzoni, Arianna Tavanti, Serena Piccinelli, Cosmeri Rizzato, Antonella Lupetti, Bruna Colombari, Eva Pericolini, Luciano Polonelli, Walter Magliani, Stefania Conti, Brunella Posteraro, Claudio Cermelli, Elisabetta Blasi, Samuele Peppoloni
Candida albicans is a commensal organism, commonly inhabiting mucosal surfaces of healthy individuals, as a part of the resident microbiota. However, in susceptible hosts, especially hospitalized and/or immunocompromised patients, it may cause a wide range of infections. The presence of abiotic substrates, such as central venous or urinary catheters, provides an additional niche for Candida attachment and persistence, particularly via biofilm development. Furthermore, Candida biofilm is poorly susceptible to most antifungals, including azoles...
2017: PloS One
https://www.readbyqxmd.com/read/28701371/population-based-assessment-of-a-biomarker-based-screening-pathway-to-aid-diagnosis-of-monogenic-diabetes-in-young-onset-patients
#3
Beverley M Shields, Maggie Shepherd, Michelle Hudson, Timothy J McDonald, Kevin Colclough, Jaime Peters, Bridget Knight, Chris Hyde, Sian Ellard, Ewan R Pearson, Andrew T Hattersley
OBJECTIVE: Monogenic diabetes, a young-onset form of diabetes, is often misdiagnosed as type 1 diabetes, resulting in unnecessary treatment with insulin. A screening approach for monogenic diabetes is needed to accurately select suitable patients for expensive diagnostic genetic testing. We used C-peptide and islet autoantibodies, highly sensitive and specific biomarkers for discriminating type 1 from non-type 1 diabetes, in a biomarker screening pathway for monogenic diabetes. RESEARCH DESIGN AND METHODS: We studied patients diagnosed aged 30 years or younger, currently younger than 50 years, in two U...
July 12, 2017: Diabetes Care
https://www.readbyqxmd.com/read/28699892/dpp-4-enzyme-deficiency-protects-kidney-from-acute-ischemia-reperfusion-injury-role-for-remote-intermittent-bowel-ischemia-reperfusion-preconditioning
#4
Yen-Ta Chen, Christopher Glenn Wallace, Chih-Chao Yang, Chih-Hung Chen, Kuan-Hung Chen, Pei-Hsun Sung, Yung-Lung Chen, Han-Tan Chai, Sheng-Ying Chung, Sarah Chua, Fan-Yen Lee, Sheung-Fat Ko, Mel S Lee, Hon-Kan Yip
We analyzed the effects of acute ischemia-reperfusion (KIR) injury on the status of kidney function and architecture in dipeptidyl peptidase4-difficient (DPP4D) rats and the effect of remote small bowel ischemia-reperfusion (BIR) preconditioning. DPP4-deficient (DPP4D) and normal Fischer344 (F344) rats were divided into 6 groups: (1) sham-F344, (2) sham-DPP4D, (3) KIR-F344 (4) KIR-DPP4D, (5) DPP4D-KIR-extendin-9-39 and (6) BIR-KIR-F344. Blood creatinine and urea nitrogen levels and the urinary protein-to-creatinine ratio was higher in KIR-F344 rats than BIR-KIR-F344 or KIR-DPP4D rats 72 h after acute KIR...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28698824/on-the-tear-proteome-of-the-house-mouse-mus-musculus-musculus-in-relation-to-chemical-signalling
#5
Romana Stopkova, Petr Klempt, Barbora Kuntova, Pavel Stopka
Mammalian tears are produced by lacrimal glands to protect eyes and may function in chemical communication and immunity. Recent studies on the house mouse chemical signalling revealed that major urinary proteins (MUPs) are not individually unique in Mus musculus musculus. This fact stimulated us to look for other sexually dimorphic proteins that may-in combination with MUPs-contribute to a pool of chemical signals in tears. MUPs and other lipocalins including odorant binding proteins (OBPs) have the capacity to selectively transport volatile organic compounds (VOCs) in their eight-stranded beta barrel, thus we have generated the tear proteome of the house mouse to detect a wider pool of proteins that may be involved in chemical signalling...
2017: PeerJ
https://www.readbyqxmd.com/read/28694024/iron-metabolism-state-of-the-art
#6
R Daher, Z Karim
Iron homeostasis relies on the amount of its absorption by the intestine and its release from storage sites, the macrophages. Iron homeostasis is also dependent on the amount of iron used for the erythropoiesis. Hepcidin, which is synthesized predominantly by the liver, is the main regulator of iron metabolism. Hepcidin reduces serum iron by inhibiting the iron exporter, ferroportin expressed both tissues, the intestine and the macrophages. In addition, in the enterocytes, hepcidin inhibits the iron influx by acting on the apical transporter, DMT1...
July 7, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28689894/urine-concentrations-of-human-beta-defensins-and-ribonuclease-7-in-urinary-tract-infection-and-asymptomatic-bacteriuria
#7
Charlott Kjölvmark, Per Åkesson, Lisa I Påhlman
Antimicrobial peptides (AMPs) provide a first line of defense against bacterial infections. Here we report that urine levels of AMPs, locally produced in the urinary tract, are lower in individuals with asymptomatic bacteriuria (ABU) compared to patients with urinary tract infection (UTI).
June 19, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28677620/ranatensin-hl-a-bombesin-related-tridecapeptide-from-the-skin-secretion-of-the-broad-folded-frog-hylarana-latouchii
#8
Yan Lin, Tianbao Chen, Mei Zhou, Lei Wang, Songkun Su, Chris Shaw
Bombesin-related peptides are a family of peptides whose prototype was discovered in amphibian skin and which exhibit a wide range of biological activities. Since the initial isolation of bombesin from Bombina bombina skin, diverse forms of bombesin-related peptides have been found in the skins across Anura. In this study, a novel bombesin-related peptide of the ranatensin subfamily, named ranatensin-HL, was structurally-characterised from the skin secretion of the broad-folded frog, Hylarana latouchii, through combination of molecular cloning and mass spectrometric methodologies...
July 4, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28675470/brain-serotoninergic-nervous-system-is-involved-in-bombesin-induced-frequent-urination-through-brain-5-ht7-receptors-in-rats
#9
Takahiro Shimizu, Shogo Shimizu, Naoki Wada, Shun Takai, Nobutaka Shimizu, Youichirou Higashi, Katsumi Kadekawa, Tsuyoshi Majima, Motoaki Saito, Naoki Yoshimura
BACKGROUND AND PURPOSE: Psychological stress exacerbates symptoms of urinary bladder dysfunction; however, underlying brain mechanisms are unclear. We reported that centrally administered bombesin, a stress-related neuropeptide, facilitates the rat micturition reflex. Brain bombesin-like peptides modulate the serotoninergic nervous system activity under stress conditions; therefore, we examined whether brain serotonin is involved in bombesin-induced frequent urination in urethane-anesthetized (0...
July 4, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28669814/quick-96fasp-for-high-throughput-quantitative-proteome-analysis
#10
Yanbao Yu, Shiferaw Bekele, Rembert Pieper
Filter aided sample preparation (FASP) is becoming a central method for proteomic sample cleanup and peptide generation prior to LC-MS analysis. We previously adapted this method to a 96-well filter plate, and applied to prepare protein digests from cell lysate and body fluid samples in a high throughput quantitative manner. While the 96FASP approach is scalable and can handle multiple samples simultaneously, two key advantages compared to single FASP, it is also time-consuming. The centrifugation-based liquid transfer on the filter plate takes 3-5 times longer than single filter...
June 29, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28655312/transplantation-of-human-fetal-pancreatic-progenitor-cells-ameliorates-renal-injury-in-streptozotocin-induced-diabetic-nephropathy
#11
Yongwei Jiang, Wenjian Zhang, Shiqing Xu, Hua Lin, Weiguo Sui, Honglin Liu, Liang Peng, Qing Fang, Li Chen, Jinning Lou
BACKGROUND: Diabetic nephropathy (DN) is a severe complication of diabetes mellitus (DM). Pancreas or islet transplantation has been reported to prevent the development of DN lesions and ameliorate or reverse existing glomerular lesions in animal models. Shortage of pancreas donor is a severe problem. Islets derived from stem cells may offer a potential solution to this problem. OBJECTIVE: To evaluate the effect of stem cell-derived islet transplantation on DN in a rat model of streptozotocin-induced DM...
June 27, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28622417/the-antimigraine-butterbur-ingredient-isopetasin-desensitises-peptidergic-nociceptors-via-the-transient-receptor-potential-ankyrin-1-channel
#12
Silvia Benemei, Francesco De Logu, Simone Li Puma, Ilaria Maddalena Marone, Elisabetta Coppi, Filippo Ugolini, Wolfgang Liedtke, Pollastro Federica, Giovanni Appendino, Pierangelo Geppetti, Serena Materazzi, Romina Nassini
BACKGROUND AND PURPOSE: The mechanism of the antimigraine action of butterbur [Petasites hybridus (L.) Gaertn.] is unknown. Here, we investigated the ability of isopetasin, a major butterbur constituent, to specifically target the transient receptor ankyrin 1 (TRPA1) channel and to affect functional responses relevant to migraine. EXPERIMENTAL APPROACH: Single cell calcium imaging and patch-clamp recordings in human and rodent TRPA1-expressing cells, neurogenic motor responses in isolated rat/mouse urinary bladder, release of calcitonin gene-related peptide (CGRP) from mouse spinal cord in vitro, and facial rubbing in mice and meningeal blood flow in rat were examined...
June 16, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28608657/maldi-tof-ms-fingerprinting-provides-evidence-of-urosepsis-caused-by-aerococcus-urinae
#13
Jieun Kim, Sung Kuk Hong, Myungsook Kim, Dongeun Yong, Kyungwon Lee
Urosepsis due to Aerococcus urinae is rare in clinical settings with only a few of reported cases worldwide by 16S rRNA sequencing. Here we report a case of sepsis caused by A. urinae in a 86 year-old male with complicated urinary tract infection which was confirmed through peptide mass fingerprinting of matrix-assisted laser desorption ionization time of flight mass spectrometry.
May 23, 2017: Infection & Chemotherapy
https://www.readbyqxmd.com/read/28589668/strongly-positive-anti-ccp-antibodies-in-patients-with-sacroiliitis-or-reactive-arthritis-post-e-%C3%A2-coli-infection-a-mini-case-series-based-review
#14
Miljyot Singh Sangha, Matthew Liam Wright, Coziana Ciurtin
We report here on four cases of patients with strongly positive anti-citrullinated cyclic peptides (anti-CCP) antibodies and clinical features of seronegative spondyloarthritis (SpA) and reactive arthritis. The four patients had various clinical presentations: one had an initial diagnosis of seropositive rheumatoid arthritis (RA) with involvement of the sacroiliac joints (similar to previous reports of the association of two diseases); one had a clinical picture of reactive arthritis following an episode of an Escherichia coli positive urinary tract infection; and two had asymmetrical sacroiliitis (SII), but no evidence of peripheral joint involvement (never reported before)...
June 6, 2017: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/28585833/energetic-basis-for-inhibition-of-calcium-phosphate-biomineralization-by-osteopontin
#15
Meng Li, Lijun Wang, Christine V Putnis
Calcium oxalate kidney stones form attached to Randall's plaques (RP), calcium phosphate (Ca-P) deposits on the renal papillary surface. Osteopontin (OPN) suppresses crystal growth in the complex process of urinary stone formation, but the inhibitory role of active domains of OPN involved in the initial formation of the RPs attached to epithelial cells has yet to be clarified. Here we demonstrate the thermodynamic basis for how OPN sequences regulate the onset of Ca-P mineral formation on lipid rafts as a model membrane...
June 12, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28576404/attenuated-diuresis-and-natriuresis-in-response-to-glucagon-like-peptide-1-in-hypertensive-rats-are-associated-with-lower-expression-of-the-glucagon-like-peptide-1-receptor-in-the-renal-vasculature
#16
Fernanda A Savignano, Renato O Crajoinas, Bruna P M Pacheco, Luciene C G Campos, Maria Heloisa M Shimizu, Antonio Carlos Seguro, Adriana C C Girardi
Accumulating evidence from clinical and experimental studies indicates that the incretin glucagon-like peptide-1 (GLP-1) elicits blood-pressure lowering effects via its diuretic, natriuretic and vasodilatory properties. The present study investigated whether acute infusion of GLP-1 induces diuresis and natriuresis in spontaneously hypertensive rats (SHRs). Additionally, we examined whether GLP-1 influences the vascular reactivity of the renal arteries of normotensive and hypertensive rats and elucidated the underlying mechanisms...
May 30, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28571926/urinary-sodium-excretion-after-gastric-bypass-surgery
#17
Neil G Docherty, Lars Fändriks, Carel W le Roux, Peter Hallersund, Malin Werling
BACKGROUND: Gut-kidney signaling is implicated in sodium homeostasis and thus blood pressure regulation. Roux-en-Y gastric bypass (RYGB) surgery for morbid obesity confers a pronounced and long-lasting blood pressure lowering effect in addition to significant weight loss. OBJECTIVES: We set out to establish whether RYGB is associated with an intrinsic change in urinary sodium excretion that may contribute to the reported blood pressure lowering effects of the procedure...
April 8, 2017: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
https://www.readbyqxmd.com/read/28531215/association-of-cord-blood-chemokines-and-other-biomarkers-with-neonatal-complications-following-intrauterine-inflammation
#18
Yoshikazu Otsubo, Kunio Hashimoto, Taro Kanbe, Muneichiro Sumi, Hiroyuki Moriuchi
BACKGROUND: Intrauterine inflammation has been associated with preterm birth and neonatal complications. Few reports have comprehensively investigated multiple cytokine profiles in cord blood and precisely identified surrogate markers for intrauterine inflammation. AIM: To identify the cytokines and surrogate markers associated with intrauterine inflammation and subsequent neonatal complications. PATIENTS AND METHODS: We analyzed cord blood samples from 135 patients admitted to the neonatal intensive care unit at Sasebo City General Hospital...
2017: PloS One
https://www.readbyqxmd.com/read/28528353/adrenomedullin-ameliorates-podocyte-injury-induced-by-puromycin-aminonucleoside-in-vitro-and-in-vivo-through-modulation-of-rho-gtpases
#19
Nan Dong, Lixia Meng, Ruqun Xue, Meng Yu, Zhonghua Zhao, Xueguang Liu
PURPOSE: Podocyte injury is a key event in proteinuric kidney disease and eventually glomerular scarring. While adrenomedullin (AM), a potent vasodilatory peptide, has been reported to confer renoprotection in several experimental models of kidney diseases, its effect on injured podocytes and the related mechanism is still largely unknown. METHODS: Employing Western blotting analysis, immunoprecipitation and immunofluorescence, we investigated the effects of AM on the expressions of podocyte cytoskeletal proteins and Rho-family small GTPases (Rho GTPases) in puromycin aminonucleoside (PAN)-induced podocyte injury, both in cultured podocytes and in PAN nephrosis rats...
May 20, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28526271/an-in-vitro-urinary-catheterization-model-that-approximates-clinical-conditions-for-evaluation-of-innovations-to-prevent-catheter-associated-urinary-tract-infections
#20
R Y R Chua, K Lim, S S J Leong, P A Tambyah, B Ho
BACKGROUND: Catheter associated urinary tract infections (CAUTI) account for about 25% of nosocomial infections globally and often result in increased morbidity and healthcare costs. An added concern is the presence of microbial biofilms which are major reservoirs of bacteria, especially antibiotic resistant bacteria, in catheters. Since the practice of the closed drainage system, innovations to combat CAUTI have not produced significant improvements in clinical outcomes. The lack of a robust laboratory platform to test new CAUTI preventive strategies may impede development of novel technologies...
May 16, 2017: Journal of Hospital Infection
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