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https://www.readbyqxmd.com/read/28819137/precise-stacking-of-decellularized-extracellular-matrix-based-3d-cell-laden-constructs-by-a-3d-cell-printing-system-equipped-with-heating-modules
#1
Geunseon Ahn, Kyung-Hyun Min, Changhwan Kim, Jeong-Seok Lee, Donggu Kang, Joo-Yun Won, Dong-Woo Cho, Jun-Young Kim, Songwan Jin, Won-Soo Yun, Jin-Hyung Shim
Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks for cell printing, e.g. inappropriate crosslinking and liquid-like rheological properties, which hinder precise 3D shaping. Therefore, in this study, we investigated the influence of various factors (e.g. bioink concentration, viscosity, and extent of crosslinking) on cell printing and established a new 3D cell printing system equipped with heating modules for the precise stacking of decellularized extracellular matrix (dECM)-based 3D cell-laden constructs...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812982/quantitative-criteria-to-benchmark-new-and-existing-bio-inks-for-cell-compatibility
#2
Karen Dubbin, Anthony Tabet, Sarah Heilshorn
Recent advancements in 3D bioprinting have led to the fabrication of more complex, more precise, and larger printed tissue constructs. As the field continues to advance, it is critical to develop quantitative benchmarks to compare different bio-inks for key cell-biomaterial interactions, including (1) cell sedimentation within the ink cartridge, (2) cell viability during extrusion, and (3) cell viability after ink curing. Here we develop three simple protocols for quantitative analysis of bio-ink performance...
August 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28806146/three-dimensional-bioprinting-of-polycaprolactone-reinforced-gene-activated-bioinks-for-bone-tissue-engineering
#3
Gráinne M Cunniffe, Tomas Gonzalez-Fernandez, Andrew Daly, Binulal N Sathy, Oju Jeon, Eben Alsberg, Daniel J Kelly
Regeneration of complex bone defects remains a significant clinical challenge. Multi-tool biofabrication has permitted the combination of various biomaterials to create multifaceted composites with tailorable mechanical properties and spatially controlled biological function. In this study we sought to use bioprinting to engineer nonviral gene activated constructs reinforced by polymeric micro-filaments. A gene activated bioink was developed using RGD-γ-irradiated alginate and nano-hydroxyapatite (nHA) complexed to plasmid DNA (pDNA)...
August 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28804996/in-vitro-evaluation-of-3d-bioprinted-tri-polymer-network-scaffolds-for-bone-tissue-regeneration
#4
Stephanie T Bendtsen, Mei Wei
In vitro evaluations provide vital information on the ability of tissue engineered scaffolds to support cell life and promote natural physiological behaviors in culture. Such assessments are necessary to conduct before implementation of the scaffolds for tissue healing in vivo. The scaffold extracellular matrix must provide the biochemical and mechanical cues necessary to promote cellular attachment, migration and proliferation before differentiation and new tissue deposition can occur. In this study, an in vitro evaluation was conducted to assess the ability of scaffolds 3D printed with a previously developed alginate-polyvinyl alcohol-hydroxyapatite formulation to promote proliferation of encapsulated MC3T3 cells...
August 14, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28804984/3d-bioprinting-for-cartilage-and-osteochondral-tissue-engineering
#5
REVIEW
Andrew C Daly, Fiona E Freeman, Tomas Gonzalez-Fernandez, Susan E Critchley, Jessica Nulty, Daniel J Kelly
Significant progress has been made in the field of cartilage and bone tissue engineering over the last two decades. As a result, there is real promise that strategies to regenerate rather than replace damaged or diseased bones and joints will one day reach the clinic however, a number of major challenges must still be addressed before this becomes a reality. These include vascularization in the context of large bone defect repair, engineering complex gradients for bone-soft tissue interface regeneration and recapitulating the stratified zonal architecture present in many adult tissues such as articular cartilage...
August 14, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28795951/gelma-collagen-blends-enable-drop-on-demand-3d-printablility-and-promote-angiogenesis
#6
Henrike Stratesteffen, Marius Johannes Köpf, Franziska Kreimendahl, Andreas Blaeser, Stefan Jockenhoevel, Horst Fischer
Effective vascularization is crucial for three dimensional (3D) printed hydrogel-cell constructs to efficiently supply cells with oxygen and nutrients. Till date, several hydrogel blends have been developed that allow the in vitro formation of a capillary-like network within the gels but comparatively less effort has been made to improve the suitability of the materials for a 3D bioprinting process. Therefore, we hypothesize that tailored hydrogel blends of photo-crosslinkable gelatin and type I collagen exhibit favorable 3D drop-on-demand printing characteristics in terms of rheological and mechanical properties and that further capillary-like network formation can be induced by co-culturing human umbilical vein endothelial cells (HUVEC) and human mesenchymal stem cells (hMSC) within the proposed blends...
August 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28790931/microtissues-enhance-smooth-muscle-differentiation-and-cell-viability-of-hadscs-for-three-dimensional-bioprinting
#7
Jin Yipeng, Xu Yongde, Wu Yuanyi, Sun Jilei, Guo Jiaxiang, Gao Jiangping, Yang Yong
Smooth muscle differentiated human adipose derived stem cells (hADSCs) provide a crucial stem cell source for urinary tissue engineering, but the induction of hADSCs for smooth muscle differentiation still has several issues to overcome, including a relatively long induction time and equipment dependence, which limits access to abundant stem cells within a short period of time for further application. Three-dimensional (3D) bioprinting holds great promise in regenerative medicine due to its controllable construction of a designed 3D structure...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28789815/bioprinting-and-cellular-therapies-for-type-1-diabetes
#8
REVIEW
Dino J Ravnic, Ashley N Leberfinger, Ibrahim T Ozbolat
Type 1 diabetes mellitus is a chronic autoimmune disease that results from the destruction of beta (β) cells in the pancreatic islets, leading to loss of insulin production and resultant hyperglycemia. Recent developments in stem cell biology have generated much excitement for β-cell replacement strategies; β cells are one of many cell types in the complex islet environment and pancreas. In this Opinion, we discuss recent successful attempts to generate β cells and how this can be coupled with bioprinting technologies in order to fabricate pancreas tissues, which holds great potential for type 1 diabetes...
August 5, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/28782725/the-bio-in-the-ink-cartilage-regeneration-with-bioprintable-hydrogels-and-articular-cartilage-derived-progenitor-cells
#9
Riccardo Levato, William R Webb, Iris A Otto, Anneloes Mensinga, Yadan Zhang, Mattie van Rijen, René van Weeren, Ilyas M Khan, Jos Malda
Cell-laden hydrogels are the primary building blocks for bioprinting, and, also termed bioinks, are the foundations for creating structures that can potentially recapitulate the architecture of articular cartilage. To be functional, hydrogel constructs need to unlock the regenerative capacity of encapsulated cells. The recent identification of multipotent articular cartilage-resident chondroprogenitor cells (ACPCs), which share important traits with adult stem cells, represents a new opportunity for cartilage regeneration...
August 4, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28782179/cytocompatibility-testing-of-hydrogels-towards-bioprinting-of-mesenchymal-stem-cells
#10
Leo Benning, Ludwig Gutzweiler, Kevin Tröndle, Julian Riba, Roland Zengerle, Peter Koltay, Stefan Zimmermann, G Björn Stark, Günter Finkenzeller
Mesenchymal stem cells (MSCs) represent a very attractive cell source for tissue engineering applications aiming at the generation of artificial bone substitutes. The use of three-dimensional (3D) bioprinting technologies has the potential to improve the classical tissue engineering approach because bioprinting will allow the generation of hydrogel scaffolds with high spatial control of MSC allocation within the bioprinted construct. In this study, we have performed direct comparisons between commercially available hydrogels in the context of their cytocompatibility towards MSCs and their physicochemical parameters with the aim to identify the most suitable hydrogel for drop-on-demand (DoD) printing of MSCs...
August 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28774034/correction-3d-bioprinting-technologies-for-hard-tissue-and-organ-engineering-materials-2016-9-802
#11
Xiaohong Wang, Qiang Ao, Xiaohong Tian, Jun Fan, Yujun Wei, Weijian Hou, Hao Tong, Shuling Bai
No abstract text is available yet for this article.
November 10, 2016: Materials
https://www.readbyqxmd.com/read/28773931/charged-triazole-cross-linkers-for-hyaluronan-based-hybrid-hydrogels
#12
Maike Martini, Patricia S Hegger, Nicole Schädel, Burcu B Minsky, Manuel Kirchhof, Sebastian Scholl, Alexander Southan, Günter E M Tovar, Heike Boehm, Sabine Laschat
Polyelectrolyte hydrogels play an important role in tissue engineering and can be produced from natural polymers, such as the glycosaminoglycan hyaluronan. In order to control charge density and mechanical properties of hyaluronan-based hydrogels, we developed cross-linkers with a neutral or positively charged triazole core with different lengths of spacer arms and two terminal maleimide groups. These cross-linkers react with thiolated hyaluronan in a fast, stoichiometric thio-Michael addition. Introducing a positive charge on the core of the cross-linker enabled us to compare hydrogels with the same interconnectivity, but a different charge density...
September 30, 2016: Materials
https://www.readbyqxmd.com/read/28773924/3d-bioprinting-technologies-for-hard-tissue-and-organ-engineering
#13
REVIEW
Xiaohong Wang, Qiang Ao, Xiaohong Tian, Jun Fan, Yujun Wei, Weijian Hou, Hao Tong, Shuling Bai
Hard tissues and organs, including the bones, teeth and cartilage, are the most extensively exploited and rapidly developed areas in regenerative medicine field. One prominent character of hard tissues and organs is that their extracellular matrices mineralize to withstand weight and pressure. Over the last two decades, a wide variety of 3D printing technologies have been adapted to hard tissue and organ engineering. These 3D printing technologies have been defined as 3D bioprinting. Especially for hard organ regeneration, a series of new theories, strategies and protocols have been proposed...
September 27, 2016: Materials
https://www.readbyqxmd.com/read/28773879/a-mathematical-model-on-the-resolution-of-extrusion-bioprinting-for-the-development-of-new-bioinks
#14
Ratima Suntornnond, Edgar Yong Sheng Tan, Jia An, Chee Kai Chua
Pneumatic extrusion-based bioprinting is a recent and interesting technology that is very useful for biomedical applications. However, many process parameters in the bioprinter need to be fully understood in order to print at an adequate resolution. In this paper, a simple yet accurate mathematical model to predict the printed width of a continuous hydrogel line is proposed, in which the resolution is expressed as a function of nozzle size, pressure, and printing speed. A thermo-responsive hydrogel, pluronic F127, is used to validate the model predictions...
September 6, 2016: Materials
https://www.readbyqxmd.com/read/28772604/special-issue-3d-printing-for-biomedical-engineering
#15
EDITORIAL
Chee Kai Chua, Wai Yee Yeong, Jia An
Three-dimensional (3D) printing has a long history of applications in biomedical engineering. The development and expansion of traditional biomedical applications are being advanced and enriched by new printing technologies. New biomedical applications such as bioprinting are highly attractive and trendy. This Special Issue aims to provide readers with a glimpse of the recent profile of 3D printing in biomedical research.
February 28, 2017: Materials
https://www.readbyqxmd.com/read/28772551/polyvinylpyrrolidone-based-bio-ink-improves-cell-viability-and-homogeneity-during-drop-on-demand-printing
#16
Wei Long Ng, Wai Yee Yeong, May Win Naing
Drop-on-demand (DOD) bioprinting has attracted huge attention for numerous biological applications due to its precise control over material volume and deposition pattern in a contactless printing approach. 3D bioprinting is still an emerging field and more work is required to improve the viability and homogeneity of printed cells during the printing process. Here, a general purpose bio-ink was developed using polyvinylpyrrolidone (PVP) macromolecules. Different PVP-based bio-inks (0%-3% w/v) were prepared and evaluated for their printability; the short-term and long-term viability of the printed cells were first investigated...
February 16, 2017: Materials
https://www.readbyqxmd.com/read/28765636/high-resolution-patterned-cellular-constructs-by-droplet-based-3d-printing
#17
Alexander D Graham, Sam N Olof, Madeline J Burke, James P K Armstrong, Ellina A Mikhailova, James G Nicholson, Stuart J Box, Francis G Szele, Adam W Perriman, Hagan Bayley
Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (10(7) cells mL(-1)) and a high droplet resolution of 1 nL...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28762957/integrating-three-dimensional-printing-and-nanotechnology-for-musculoskeletal-regeneration
#18
Margaret Nowicki, Nathan Castro, Raj Rao, Michael Plesniak, Lijie Grace Zhang
The field of tissue engineering is advancing steadily, partly due to advancements in rapid prototyping technology. Even with increasing focus, successful complex tissue regeneration of vascularized bone, cartilage and the osteochondral interface remains largely illusive. This review examines current three-dimensional (3D) bioprinting techniques and their application towards bone, cartilage and osteochondral regeneration. The importance of, and benefit to, nanomaterial integration is also highlighted with recent published examples...
August 1, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28756658/polymers-for-3d-printing-and-customized-additive-manufacturing
#19
Samuel Clark Ligon, Robert Liska, Jürgen Stampfl, Matthias Gurr, Rolf Mülhaupt
Additive manufacturing (AM) alias 3D printing translates computer-aided design (CAD) virtual 3D models into physical objects. By digital slicing of CAD, 3D scan, or tomography data, AM builds objects layer by layer without the need for molds or machining. AM enables decentralized fabrication of customized objects on demand by exploiting digital information storage and retrieval via the Internet. The ongoing transition from rapid prototyping to rapid manufacturing prompts new challenges for mechanical engineers and materials scientists alike...
August 9, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28737701/application-of-extrusion-based-hydrogel-bioprinting-for-cartilage-tissue-engineering
#20
REVIEW
Fu You, B Frank Eames, Xiongbiao Chen
Extrusion-based bioprinting (EBB) is a rapidly developing technique that has made substantial progress in the fabrication of constructs for cartilage tissue engineering (CTE) over the past decade. With this technique, cell-laden hydrogels or bio-inks have been extruded onto printing stages, layer-by-layer, to form three-dimensional (3D) constructs with varying sizes, shapes, and resolutions. This paper reviews the cell sources and hydrogels that can be used for bio-ink formulations in CTE application. Additionally, this paper discusses the important properties of bio-inks to be applied in the EBB technique, including biocompatibility, printability, as well as mechanical properties...
July 23, 2017: International Journal of Molecular Sciences
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