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Liqun Ning, Haoying Sun, Tiphanie Lelong, Romain Guilloteau, Ning Zhu, David J Schreyer, Daniel Xiongbiao Chen
Three-dimensional (3D) bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve regeneration, yet remain unachievable. This paper presents our study on bioprinting Schwann cell-encapsulated scaffolds using composite hydrogels of alginate, fibrin, hyaluronic acid, and/or RGD peptide, for nerve tissue engineering applications...
June 18, 2018: Biofabrication
Mylène de Ruijter, Alexandre Ribeiro, Inge Dokter, Miguel Castilho, Jos Malda
Fabrication of biomimetic tissues holds much promise for the regeneration of cells or organs that are lost or damaged due to injury or disease. To enable the generation of complex, multicellular tissues on demand, the ability to design and incorporate different materials and cell types needs to be improved. Two techniques are combined: extrusion-based bioprinting, which enables printing of cell-encapsulated hydrogels; and melt electrowriting (MEW), which enables fabrication of aligned (sub)-micrometer fibers into a single-step biofabrication process...
June 17, 2018: Advanced Healthcare Materials
Elliot S Bishop, Sami Mostafa, Mikhail Pakvasa, Hue H Luu, Michael J Lee, Jennifer Moriatis Wolf, Guillermo A Ameer, Tong-Chuan He, Russell R Reid
Advances in three-dimensional (3D) printing have increased feasibility towards the synthesis of living tissues. Known as 3D bioprinting, this technology involves the precise layering of cells, biologic scaffolds, and growth factors with the goal of creating bioidentical tissue for a variety of uses. Early successes have demonstrated distinct advantages over conventional tissue engineering strategies. Not surprisingly, there are current challenges to address before 3D bioprinting becomes clinically relevant...
December 2017: Genes & Diseases
Derek M van Pel, Kaori Harada, Dandan Song, Christian C Naus, Wun Chey Sin
Glioma is a highly aggressive form of brain cancer, with some subtypes having 5-year survival rates of less than 5%. Tumour cell invasion into the surrounding parenchyma seems to be the primary driver of these poor outcomes, as most gliomas recur within 2 cm of the original surgically-resected tumour. Many current approaches to the development of anticancer therapy attempt to target genetic weaknesses in a particular cancer, but may not take into account the microenvironment experienced by a tumour and the patient-specific genetic differences in susceptibility to treatment...
June 16, 2018: Journal of Cell Communication and Signaling
Pallab Datta, Ananya Barui, Yang Wu, Veli Ozbolat, Kazim K Moncal, Ibrahim T Ozbolat
An increasing demand for directed assembly of biomaterials has inspired the development of bioprinting, which facilitates the assembling of both cellular and acellular inks into well-arranged three-dimensional (3D) structures for tissue fabrication. Although great advances have been achieved in the recent decade, there still exist issues to be addressed. Herein, a review has been systematically performed to discuss the considerations in the entire procedure of bioprinting. Though bioprinting is advancing at a rapid pace, it is seen that the whole process of obtaining tissue constructs from this technique involves multiple-stages, cutting across various technology domains...
June 14, 2018: Biotechnology Advances
Martin Raasch, Enrico Fritsche, Andreas Kurtz, Michael Bauer, Alexander S Mosig
Complex cell culture models such as microphysiological models (MPS) mimicking human liver functionality in vitro are in the spotlight as alternative to conventional cell culture and animal models. Promising techniques like microfluidic cell culture or micropatterning by 3D bioprinting are gaining increasing importance for the development of MPS to address the needs for more predictivity and cost efficiency. In this context, human induced pluripotent stem cells (hiPSCs) offer new perspectives for the development of advanced liver-on-chip systems by recreating an in vivo like microenvironment that supports the reliable differentiation of hiPSCs to hepatocyte-like cells (HLC)...
June 14, 2018: Advanced Drug Delivery Reviews
Nicole Rotter, Marcy Zenobi-Wong
Regeneration as a therapeutic priniciple and regenerative medicine in general are promising new strategies to add new therapeutic dimensions to our current treatment options. Today, reconstructive surgery, drugs and implants such as the cochlear implant can replace the functions of damaged tissues. In contrast, regenerative therapies aim at the replacement of the damaged tissues themselves while at the same time replacing their lost tissue function. In this review article new technologies such as 3D-bioprinting and the application of decellularised tissues as biomaterials are introduced and explained...
March 2018: Laryngo- Rhino- Otologie
Lokesh Karthik Narayanan, Trevor L Thompson, Rohan A Shirwaiker, Binil Starly
Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. In this work, we investigate the dielectric response of viable cells encapsulated in bioprinted 3D hydrogel constructs to an applied alternating electric field as a label-free non-destructive monitoring approach...
June 14, 2018: Biofabrication
Sandra Laternser, Hansjoerg Keller, Olivier Leupin, Martin Rausch, Ursula Graf-Hausner, Markus Rimann
Two-dimensional (2D) cell cultures do not reflect the in vivo situation, and thus it is important to develop predictive three-dimensional (3D) in vitro models with enhanced reliability and robustness for drug screening applications. Treatments against muscle-related diseases are becoming more prominent due to the growth of the aging population worldwide. In this study, we describe a novel drug screening platform with automated production of 3D musculoskeletal-tendon-like tissues. With 3D bioprinting, alternating layers of photo-polymerized gelatin-methacryloyl-based bioink and cell suspension tissue models were produced in a dumbbell shape onto novel postholder cell culture inserts in 24-well plates...
June 1, 2018: SLAS Technology
A Béduer, N Piacentini, L Aeberli, A Da Silva, C A Verheyen, F Bonini, A Rochat, A Filippova, L Serex, P Renaud, T Braschler
We present a 3D-printing technology allowing free-form fabrication of centimetre-scale injectable structures for minimally invasive delivery. They result from the combination of 3D printing onto a cryogenic substrate and optimisation of carboxymethylcellulose-based cryogel inks. The resulting highly porous and elastic cryogels are biocompatible, and allow for protection of cell viability during compression for injection. Implanted into the murine subcutaneous space, they are colonized with a loose fibrovascular tissue with minimal signs of inflammation and remain encapsulation-free at three months...
June 5, 2018: Acta Biomaterialia
Stephen William Sawyer, Shivkumar Vishnempet Shridhar, Kairui Zhang, Lucas Albrecht, Alex Filip, Jason Horton, Pranav Soman
Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol (PVA) pipes were used to create perfusable channels...
June 8, 2018: Biofabrication
Jorge A Belgodere, Connor T King, Jacob B Bursavich, Matthew E Burow, Elizabeth C Martin, Jangwook P Jung
The extracellular matrix (ECM) is a critical cue to direct tumorigenesis and metastasis. Although two-dimensional (2D) culture models have been widely employed to understand breast cancer microenvironments over the past several decades, the 2D models still exhibit limited success. Overwhelming evidence supports that three dimensional (3D), physiologically relevant culture models are required to better understand cancer progression and develop more effective treatment. Such platforms should include cancer-specific architectures, relevant physicochemical signals, stromal-cancer cell interactions, immune components, vascular components, and cell-ECM interactions found in patient tumors...
2018: Frontiers in Bioengineering and Biotechnology
Jia Shi, Bin Wu, Bin Song, Jinchun Song, Shihao Li, Dieter Trau, Wen F Lu
Drop-on-demand (DOD) printing is widely used in bioprinting for tissue engineering because of little damage to cell viability and cost-effectiveness. However, satellite droplets may be generated during printing, deviating cells from the desired position and affecting printing position accuracy. Current control on cell injection in DOD printing is primarily based on trial-and-error process, which is time-consuming and inflexible. In this paper, a novel machine learning technology based on Learning-based Cell Injection Control (LCIC) approach is demonstrated for effective DOD printing control while eliminating satellite droplets automatically...
June 5, 2018: Annals of Biomedical Engineering
Sang-Woo Bae, Kang-Woog Lee, Jae-Hyun Park, JunHee Lee, Cho-Rok Jung, JunJie Yu, Hwi-Yool Kim, Dae-Hyun Kim
Tracheal resection has limited applicability. Although various tracheal replacement strategies were performed using artificial prosthesis, synthetic stents and tissue transplantation, the best method in tracheal reconstruction remains to be identified. Recent advances in tissue engineering enabled 3D bioprinting using various biocompatible materials including living cells, thereby making the product clinically applicable. Moreover, clinical interest in mesenchymal stem cell has dramatically increased. Here, rabbit bone marrow-derived mesenchymal stem cells (bMSC) and rabbit respiratory epithelial cells were cultured...
May 31, 2018: International Journal of Molecular Sciences
Chin Siang Ong, Lucy Nam, Kingsfield Ong, Aravind Krishnan, Chen Yu Huang, Takuma Fukunishi, Narutoshi Hibino
3D and 4D bioprinting of the heart are exciting notions in the modern era. However, myocardial bioprinting has proven to be challenging. This review outlines the methods, materials, cell types, issues, challenges, and future prospects in myocardial bioprinting. Advances in 3D bioprinting technology have significantly improved the manufacturing process. While scaffolds have traditionally been utilized, 3D bioprinters, which do not require scaffolds, are increasingly being employed. Improved understanding of the cardiac cellular composition and multiple strategies to tackle the issues of vascularization and viability had led to progress in this field...
2018: BioMed Research International
Ross A Petrella, Pete A Mollica, Martina Zamponi, John A Reid, Shu Xiao, Robert D Bruno, Patrick C Sachs
Picosecond pulse electric fields (psPEF) have the potential to elicit functional changes in mammalian cells in a non-contact manner. Such electro-manipulation of pluripotent and multipotent cells could be a tool in both neural interface and tissue engineering. Here, we describe the potential of psPEF in directing neural stem cells (NSCs) gene expression, metabolism, and proliferation. As a comparison mesenchymal stem cells (MSCs) were also tested.
 Approach. A psPEF electrode was anchored on a customized commercially available 3-D printer, which allowed us to deliver pulses with high spatial precision and systematically control the electrode position in three-axes...
May 31, 2018: Journal of Neural Engineering
Jasmine O Castro, Shwathy Ramesan, Amgad R Rezk, Leslie Y Yeo
We report a miniaturised platform for continuous production of single or multiple liquid droplets with diameters between 60 and 500 μm by interfacing a capillary-driven self-replenishing liquid feed with pulsed excitation of focussed surface acoustic waves (SAWs). The orifice-free operation circumvents the disadvantages of conventional jetting systems, which are often prone to clogging that eventuates in rapid degradation of the operational performance. Additionally, we show the possibility for flexibly tuning the ejected droplet size through the pulse width duration, thus avoiding the need for a separate device for every different droplet size required, as is the case for systems in which the droplet size is set by nozzles and orifices, as well as preceding ultrasonic jetting platforms where the droplet size is controlled by the operating frequency...
May 30, 2018: Soft Matter
Dianjun Qi, Shaohua Wu, Mitchell A Kuss, Wen Shi, Soonkyu Chung, Paul T Deegan, Alexey Kamenskiy, Yini He, Bin Duan
Bioengineered adipose tissues have gained increased interest as a promising alternative to autologous tissue flaps and synthetic adipose fillers for soft tissue augmentation and defect reconstruction in clinic. Although many scaffolding materials and biofabrication methods have been investigated for adipose tissue engineering in the last decades, there are still challenges to recapitulate the appropriate adipose tissue microenvironment, maintain volume stability, and induce vascularization to achieve long-term function and integration...
May 26, 2018: Acta Biomaterialia
Johan Göhl, Kajsa Markstedt, Andreas Mark, Karl M O Håkansson, Paul Gatenholm, Fredrik Edelvik
3D bioprinting with bioinks that contain cells show great promise in the biofabrication of patient specific tissue constructs. To fulfill the multiple requirements of a bioink, a wide range of materials and bioink composition are being developed and evaluated with regard to cell viability, mechanical performance and printability. It is essential that the printability and printing fidelity is not neglected since failure in printing the targeted architecture may be catastrophic for the survival of the cells and consequently the function of the printed tissue...
May 29, 2018: Biofabrication
Taufiq Ahmad, Hyeok Jun Shin, Jinkyu Lee, Young Min Shin, Sajeesh Kumar Madhurakat Perikamana, So Yeon Park, Hyun Suk Jung, Heungsoo Shin
Development of a bone-like 3D microenvironment with stem cells has always been intriguing in bone tissue engineering. In this study, we fabricated composite spheroids by combining functionalized fibers and human adipose-derived stem cells (hADSCs), which were fused to form a 3D mineralized tissue construct. We prepared fragmented poly (ι-lactic acid) (PLLA) fibers approximately 100 μm long by partial aminolysis of electrospun fibrous mesh. PLLA fibers were then biomineralized with various concentrations of NaHCO3 (0...
May 23, 2018: Acta Biomaterialia
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