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cardiac tissue engineering

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https://www.readbyqxmd.com/read/28934329/biochemical-and-biomechanical-properties-of-the-pacemaking-sinoatrial-node-extracellular-matrix-are-distinct-from-contractile-left-ventricular-matrix
#1
Jessica M Gluck, Anthony W Herren, Sergey Yechikov, Hillary K J Kao, Ambereen Khan, Brett S Phinney, Nipavan Chiamvimonvat, James W Chan, Deborah K Lieu
Extracellular matrix plays a role in differentiation and phenotype development of its resident cells. Although cardiac extracellular matrix from the contractile tissues has been studied and utilized in tissue engineering, extracellular matrix properties of the pacemaking sinoatrial node are largely unknown. In this study, the biomechanical properties and biochemical composition and distribution of extracellular matrix in the sinoatrial node were investigated relative to the left ventricle. Extracellular matrix of the sinoatrial node was found to be overall stiffer than that of the left ventricle and highly heterogeneous with interstitial regions composed of predominantly fibrillar collagens and rich in elastin...
2017: PloS One
https://www.readbyqxmd.com/read/28924210/kinase-inhibitor-screening-using-artificial-neural-networks-and-engineered-cardiac-biowires
#2
Genevieve Conant, Samad Ahadian, Yimu Zhao, Milica Radisic
Kinase inhibitors are often used as cancer targeting agents for their ability to prevent the activation of cell growth and proliferation signals. Cardiotoxic effects have been identified for some marketed kinase inhibitors that were not detected during clinical trials. We hypothesize that more predictive cardiac functional assessments of kinase inhibitors on human myocardium can be established by combining a high-throughput two-dimensional (2D) screening assay and a high-content three-dimensional (3D) engineered cardiac tissue (Biowire(TM)) based assay, and using human induced pluripotent stem cell-derived CMs (hiPSC-CMs)...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923324/regenerating-heart-using-a-novel-compound-and-human-wharton-jelly-mesenchymal-stem-cells
#3
Shahram Rabbani, Masoud Soleimani, Mohammad Imani, Mohammad Sahebjam, Ali Ghiaseddin, Seyed Mahdi Nassiri, Jalil Majd Ardakani, Maryam Tajik Rostami, Arash Jalali, Bahmanshir Mousanassab, Mahsa Kheradmandi, Seyed Hossein Ahmadi Tafti
BACKGROUND: Myocardial infarction is a major problem in health system and most conventional therapy is not led to restoration of the health. Stem cell therapy is a method to regenerate the heart but today appropriate cell source and scaffold selection as extracellular matrix to achieve the best effect is disputing. AIM OF THE STUDY: In this study a combination of human Wharton jelly mesenchymal stem cells (HWJMSCs) with a novel compound consisting polyethylene glycol (PEG), hyaluronic acid and chitosan is presented to heart regeneration...
April 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/28900152/a-3d-magnetic-tissue-stretcher-for-remote-mechanical-control-of-embryonic-stem-cell-differentiation
#4
Vicard Du, Nathalie Luciani, Sophie Richard, Gaëtan Mary, Cyprien Gay, François Mazuel, Myriam Reffay, Philippe Menasché, Onnik Agbulut, Claire Wilhelm
The ability to create a 3D tissue structure from individual cells and then to stimulate it at will is a major goal for both the biophysics and regenerative medicine communities. Here we show an integrated set of magnetic techniques that meet this challenge using embryonic stem cells (ESCs). We assessed the impact of magnetic nanoparticles internalization on ESCs viability, proliferation, pluripotency and differentiation profiles. We developed magnetic attractors capable of aggregating the cells remotely into a 3D embryoid body...
September 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/28898757/extracellular-matrix-derived-extracellular-vesicles-promote-cardiomyocyte-growth-and-electrical-activity-in-engineered-cardiac-atria
#5
Minae An, Kihwan Kwon, Junbeom Park, Dong-Ryeol Ryu, Jung-A Shin, Jihee Lee Kang, Ji Ha Choi, Eun-Mi Park, Kyung Eun Lee, Minna Woo, Minsuk Kim
Extracellular matrix (ECM) plays a critical role in the provision of the necessary microenvironment for the proper regeneration of the cardiac tissue. However, specific mechanisms that lead to ECM-mediated cardiac regeneration are not well understood. To elucidate the potential mechanisms, we investigated ultra-structures of the cardiac ECM using electron microscopy. Intriguingly, we observed large quantities of micro-vesicles from decellularized right atria. RNA and protein analyses revealed that these contained exosomal proteins and microRNAs (miRNAs), which we referred to herein as ECM-derived extracellular vesicles (ECM-EVs)...
September 4, 2017: Biomaterials
https://www.readbyqxmd.com/read/28895502/characterization-of-the-epicardial-adipose-tissue-in-decellularized-human-scaled-whole-hearts-implications-for-the-whole-heart-tissue-engineering
#6
Payam Akhyari, Fabian Oberle, Joern Huelsmann, Hans Heid, Stefan Lehr, Andreas Barbian, Sentaro Nakanishi, Hug Aubin, Alexander Jenke, Artur Lichtenberg
Whole-organ engineering is an innovative field of regenerative medicine with growing translational perspectives. Recent reports suggest the feasibility of decellularization and repopulation of entire human size hearts. However, little is known about the susceptibility of epicardial adipose tissue (EAT) to decellularization. Here, human size hearts of ovine donors were subjected to perfusion-based decellularization using detergent solutions. Upon basic histological evaluation and total DNA measurement myocardial regions prove largely decellularized while EAT demonstrated cellular remnants, further confirmed by transmission electron microscopy...
September 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28895403/injectable-carbon-nanotube-functionalized-reverse-thermal-gel-promotes-cardiomyocytes-survival-and-maturation
#7
Brisa Peña, Susanna Bosi, Brian A Aguado, Daniele Borin, Nikki L Farnsworth, Evgenia Dobrinskikh, Teisha J Rowland, Valentina Martinelli, Mark Jeong, Matthew R G Taylor, Carlin S Long, Robin Shandas, Orfeo Sbaizero, Maurizio Prato, Kristi S Anseth, Daewon Park, Luisa Mestroni
The ability of the adult heart to regenerate cardiomyocytes (CMs) lost after injury is limited, generating interest in developing efficient cell-based transplantation therapies. Rigid carbon nanotubes (CNTs) scaffolds have been used to improve CMs viability, proliferation, and maturation, but they require undesirable invasive surgeries for implantation. To overcome this limitation, we developed an injectable reverse thermal gel (RTG) functionalized with CNTs (RTG-CNT) that transitions from a solution at room temperature to a three-dimensional (3D) gel-based matrix shortly after reaching body temperature...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28892929/connective-tissue-growth-factor-transgenic-mouse-develops-cardiac-hypertrophy-lean-body-mass-and-alopecia
#8
Edem Nuglozeh
INTRODUCTION: Connective Tissue Growth Factor (CTGF/CCN2) is one of the six members of cysteine-rich, heparin-binding proteins, secreted as modular protein and recognised to play a major function in cell processes such as adhesion, migration, proliferation and differentiation as well as chondrogenesis, skeletogenesis, angiogenesis and wound healing. The capacity of CTGF to interact with different growth factors lends an important role during early and late development, especially in the anterior region of the embryo...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28888004/cardiomyocyte-coculture-on-layered-fibrous-scaffolds-assembled-from-micropatterned-electrospun-mats
#9
Yaowen Liu, Guisen Xu, Jiaojun Wei, Qiang Wu, Xiaohong Li
Challenges remain in engineering cardiac tissues with functional and morphological properties similar to those of native myocardium. In the current study, micropatterned fibrous mats are obtained by deposition of electrospun fibers on lithographic collectors to reproduce the anisotropic structure of myocardium, and carbon nanotubes are included in fibers to provide conductivities at the same level of cardiac muscles. The patterned mats are assembled layer-by-layer into patterned scaffolds for coculture of primary cardiomyocytes (CMs) with cardiac fibroblasts (CFs) and endothelial cells (ECs)...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28875579/a-cardiac-patch-from-aligned-microvessel-and-cardiomyocyte-patches
#10
Jeremy A Schaefer, Pilar A Guzman, Sonja B Riemenschneider, Timothy J Kamp, Robert T Tranquillo
Cardiac tissue engineering aims to produce replacement tissue patches in the lab to replace or treat infarcted myocardium. However, current patches lack pre-formed microvascularization and are therefore limited in thickness and force production. In the present study, we sought to assess whether a bi-layer patch composed of a layer made from human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and a microvessel layer composed of self-assembled human blood outgrowth endothelial cells (BOECs) and pericytes (PCs) was capable of engrafting on the epicardial surface of a nude rat infarct model and becoming perfused by the host four weeks after acute implantation...
September 5, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28875152/artificial-cardiac-muscle-with-or-without-the-use-of-scaffolds
#11
REVIEW
Yifei Li, Donghui Zhang
During the past several decades, major advances and improvements now promote better treatment options for cardiovascular diseases. However, these diseases still remain the single leading cause of death worldwide. The rapid development of cardiac tissue engineering has provided the opportunity to potentially restore the contractile function and retain the pumping feature of injured hearts. This conception of cardiac tissue engineering can enable researchers to produce autologous and functional biomaterials which represents a promising technique to benefit patients with cardiovascular diseases...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28857113/myofibrils-in-cardiomyocytes-tend-to-assemble-along-the-maximal-principle-stress-directions
#12
Hongyan Yuan, Bahador Marzban, Kevin Kit Parker
The mechanisms underlying the spatial organization of self-assembled myofibrils in cardiac tissues remain incompletely understood. By modeling cells as elastic solids under active cytoskeletal contraction, we found a good correlation between the predicted maximal principal stress directions and the in vitro myofibril orientations in individual cardiomyocytes. This implies that actomyosin fibers tend to assemble along the maximal tensile stress directions. By considering the dynamics of focal adhesion and myofibril formation in the model, we showed that the different patterns of myofibril organizations in mature versus immature cardiomyocytes can be explained as the consequence of the different levels of force-dependent remodeling of focal adhesions...
August 31, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28855904/immunological-properties-of-murine-parthenogenetic-stem-cell-derived-cardiomyocytes-and-engineered-heart-muscle
#13
Michael Didié, Satish Galla, Vijayakumar Muppala, Ralf Dressel, Wolfram-Hubertus Zimmermann
Pluripotent parthenogenetic stem cells (pSCs) can be derived by pharmacological activation of unfertilized oocytes. Homozygosity of the major histocompatibility complex (MHC) in pSCs makes them an attractive cell source for applications in allogeneic tissue repair. This was recently demonstrated for pSC-based tissue-engineered heart repair. A detailed analysis of immunological properties of pSC-derived cardiomyocytes and engineered heart muscle (EHM) thereof is, however, lacking. The aim of this study was to determine baseline and cytokine-inducible MHC class I and MHC class II as well as programmed death ligand-1 (PDL-1) and co-stimulatory protein (CD40, CD80, CD86) expression in pSC-derived cardiomyocytes and pSC-EHM in vitro and in vivo...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28854488/histological-and-biomechanical-characterization-of-decellularized-porcine-pericardium-as-a-potential-scaffold-for-tissue-engineering-applications
#14
Panagiotis Mallis, Efstathios Michalopoulos, Constantine Dimitriou, Nikolaos Kostomitsopoulos, Catherine Stavropoulos-Giokas
BACKGROUND: Each year, more than 800,000 vascular and cardiac surgeries are performed therefore, there is a great need for suitable material for bioprosthetic operations. Porcine pericardium is a double-walled sac that covers the heart and can be used in vascular and cardiac thoracic surgery. OBJECTIVE: The aim of the present study was to evaluate the decellularization process and biomechanical properties in porcine pericardial tissue after the decellularization treatment...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28832034/surface-modified-polymers-for-cardiac-tissue-engineering
#15
REVIEW
Ambigapathi Moorthi, Yu-Chang Tyan, Tze-Wen Chung
Cardiovascular disease (CVD), leading to myocardial infarction and heart failure, is one of the major causes of death worldwide. The physiological system cannot significantly regenerate the capabilities of a damaged heart. The current treatment involves pharmacological and surgical interventions; however, less invasive and more cost-effective approaches are sought. Such new approaches are developed to induce tissue regeneration following injury. Hence, regenerative medicine plays a key role in treating CVD...
August 23, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28826352/pcl-pdms-pcl-copolymer-based-microspheres-mediate-cardiovascular-differentiation-from-embryonic-stem-cells
#16
Liqing Song, Mohammad Faisal Ahmed, Yan Li, Julie Bejoy, Changchun Zeng, Yan Li
Poly-ϵ-caprolactone (PCL) based microspheres have received much attention as drug or growth factor delivery carriers and tissue engineering scaffolds due to their biocompatibility, biodegradability, and tunable biophysical properties. In addition, PCL and polydimethylsiloxane (PDMS) can be fabricated into thermo-responsive shape memory polymers for various biomedical applications (e.g., smart sutures and vascular stents). However, the influence of biophysical properties of PCL-PDMS based microspheres on stem cell lineage commitment has not been well understood...
August 21, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28805824/flexible-shape-memory-scaffold-for-minimally-invasive-delivery-of-functional-tissues
#17
Miles Montgomery, Samad Ahadian, Locke Davenport Huyer, Mauro Lo Rito, Robert A Civitarese, Rachel D Vanderlaan, Jun Wu, Lewis A Reis, Abdul Momen, Saeed Akbari, Aric Pahnke, Ren-Ke Li, Christopher A Caldarone, Milica Radisic
Despite great progress in engineering functional tissues for organ repair, including the heart, an invasive surgical approach is still required for their implantation. Here, we designed an elastic and microfabricated scaffold using a biodegradable polymer (poly(octamethylene maleate (anhydride) citrate)) for functional tissue delivery via injection. The scaffold's shape memory was due to the microfabricated lattice design. Scaffolds and cardiac patches (1 cm × 1 cm) were delivered through an orifice as small as 1 mm, recovering their initial shape following injection without affecting cardiomyocyte viability and function...
August 14, 2017: Nature Materials
https://www.readbyqxmd.com/read/28781077/embryonic-stem-cell-differentiation-to-functional-arterial-endothelial-cells-through-sequential-activation-of-etv2-and-notch1-signaling-by-hif1%C3%AE
#18
Kit Man Tsang, James S Hyun, Kwong Tai Cheng, Micaela Vargas, Dolly Mehta, Masuko Ushio-Fukai, Li Zou, Kostandin V Pajcini, Jalees Rehman, Asrar B Malik
The generation of functional arterial endothelial cells (aECs) from embryonic stem cells (ESCs) holds great promise for vascular tissue engineering. However, the mechanisms underlying their generation and the potential of aECs in revascularizing ischemic tissue are not fully understood. Here, we observed that hypoxia exposure of mouse ESCs induced an initial phase of HIF1α-mediated upregulation of the transcription factor Etv2, which in turn induced the commitment to the EC fate. However, sustained activation of HIF1α in these EC progenitors thereafter induced NOTCH1 signaling that promoted the transition to aEC fate...
September 12, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28780562/rationale-and-design-of-a-multicentre-prospective-randomised-controlled-clinical-trial-to-evaluate-the-efficacy-of-the-adipose-graft-transposition-procedure-in-patients-with-a-myocardial-scar-the-agtp-ii-trial
#19
Paloma Gastelurrutia, Carolina Gálvez-Montón, Maria Luisa Cámara, Juan Bustamante-Munguira, Pablo García-Pavia, Pablo Avanzas, J Alberto San Román, Domingo Pascual-Figal, Eduardo de Teresa, Maria G Crespo-Leiro, Nicolás Manito, Julio Núñez, Francisco Fernández-Avilés, Ángel Caballero, Albert Teis, Josep Lupón, Ramón Brugada, Carlos Martín, Jacobo Silva, Ana Revilla-Orodea, Sergio J Cánovas, Jose M Melero, Jose J Cuenca-Castillo, Angel Gonzalez-Pinto, Antoni Bayes-Genis
INTRODUCTION: Cardiac adipose tissue is a source of progenitor cells with regenerative capacity. Studies in rodents demonstrated that the intramyocardial delivery of cells derived from this tissue improves cardiac function after myocardial infarction (MI). We developed a new reparative approach for damaged myocardium that integrates the regenerative properties of cardiac adipose tissue with tissue engineering. In the adipose graft transposition procedure (AGTP), we dissect a vascularised flap of autologous pericardial adipose tissue and position it over the myocardial scarred area...
August 4, 2017: BMJ Open
https://www.readbyqxmd.com/read/28774384/overcoming-the-roadblocks-to-cardiac-cell-therapy-using-tissue-engineering
#20
REVIEW
Mounica Yanamandala, Wuqiang Zhu, Daniel J Garry, Timothy J Kamp, Joshua M Hare, Ho-Wook Jun, Young-Sup Yoon, Nenad Bursac, Sumanth D Prabhu, Gerald W Dorn, Roberto Bolli, Richard N Kitsis, Jianyi Zhang
Transplantations of various stem cells or their progeny have repeatedly improved cardiac performance in animal models of myocardial injury; however, the benefits observed in clinical trials have been generally less consistent. Some of the recognized challenges are poor engraftment of implanted cells and, in the case of human cardiomyocytes, functional immaturity and lack of electrical integration, leading to limited contribution to the heart's contractile activity and increased arrhythmogenic risks. Advances in tissue and genetic engineering techniques are expected to improve the survival and integration of transplanted cells, and to support structural, functional, and bioenergetic recovery of the recipient hearts...
August 8, 2017: Journal of the American College of Cardiology
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