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cardiac tissue engineering

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https://www.readbyqxmd.com/read/28227439/three-dimensional-graphene-scaffold-for-cardiac-tissue-engineering-and-in-situ-electrical-recording
#1
S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, S K Ameri, P K Singh, R D'Angelo, W Stoppel, L Black, S R Sonkusale, L Black, P K Singh, W Stoppel, S R Sonkusale, R D'Angelo, S K Ameri
In this paper, we present a three-dimensional graphene foam made of few layers of CVD grown graphene as a scaffold for growing cardiac cells and recording their electrical activity. Our results show that graphene foam not only provides an excellent extra-cellular matrix (ECM) for the culture of such electrogenic cells but also enables recording of its extracellular electrical activity in-situ. Recording is possible due to graphene's excellent conductivity. In this paper, we present our results on the fabrication of the graphene scaffold and initial studies on the culture of cardiac cell lines such as HL-1 and recording of their real-time electrical activity...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28220995/development-of-hydrogels-for-regenerative-engineering
#2
REVIEW
Xiaofei Guan, Meltem Avci-Adali, Emine Alarçin, Hao Cheng, Sara Saheb Kashaf, Yuxiao Li, Aditya Chawla, Hae Lin Jang, Ali Khademhosseini
The aim of regenerative engineering is to restore complex tissues and biological systems through convergence in the fields of advanced biomaterials, stem cell science, and developmental biology. Hydrogels are one of the most attractive biomaterials for regenerative engineering, since they can be engineered into tissue mimetic 3D scaffolds to support cell growth due to their similarity to native extracellular matrix. Advanced nano- and micro-technologies have dramatically increased the ability to control properties and functionalities of hydrogel materials by facilitating biomimetic fabrication of more sophisticated compositions and architectures, thus extending our understanding of cell-matrix interactions at the nanoscale...
February 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28215153/engineered-muscle-tissues-for-disease-modeling-and-drug-screening-applications
#3
Mohammad Hossein Mohammadi, Raquel Obregón, Samad Ahadian, Javier Ramón-Azcón, Milica Radisic
Animal models have been the main resources for drug discovery and prediction of drugs' pharmacokinetic responses in the body. However, noticeable drawbacks associated with animal models include high cost, low reproducibility, low physiological similarity to humans, and ethical problems. Engineered tissue models have recently emerged as an alternative or substitute for animal models in drug discovery and testing and disease modeling. In this review, we focus on skeletal muscle and cardiac muscle tissues by first describing their characterization and physiology...
February 15, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28195834/surface-acoustic-waves-induced-micropatterning-of-cells-in-gelatin-methacryloyl-gelma-hydrogels
#4
Shahid M Naseer, Amir Manbachi, Mohamadmahdi Samandari, Philipp Walch, Yuan Gao, Yu Shrike Zhang, Farideh Davoudi, Wesley Wang, Karen Abrinia, Jonathan M Cooper, Ali Khademhosseini, Su Ryon Shin
Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force patterning for the rapid arrangement of biological objects, such as cells, in a three-dimensional (3D) environment. In this study, we report on a bio-acoustic force patterning technique, which uses surface acoustic waves (SAWs) for the rapid arrangement of cells within an extracellular matrix-based hydrogel such as gelatin methacryloyl (GelMA)...
February 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28194397/a-novel-human-tissue-engineered-3-d-functional-vascularized-cardiac-muscle-construct
#5
Mani T Valarmathi, John W Fuseler, Jeffrey M Davis, Robert L Price
Organ tissue engineering, including cardiovascular tissues, has been an area of intense investigation. The major challenge to these approaches has been the inability to vascularize and perfuse the in vitro engineered tissue constructs. Attempts to provide oxygen and nutrients to the cells contained in the biomaterial constructs have had varying degrees of success. The aim of this current study is to develop a three-dimensional (3-D) model of vascularized cardiac tissue to examine the concurrent temporal and spatial regulation of cardiomyogenesis in the context of postnatal de novo vasculogenesis during stem cell cardiac regeneration...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28191775/noninvasive-assessment-of-an-engineered-bioactive-graft-in-myocardial-infarction-impact-on-cardiac-function-and-scar-healing
#6
Carolina Gálvez-Montón, Ramon Bragós, Carolina Soler-Botija, Idoia Díaz-Güemes, Cristina Prat-Vidal, Verónica Crisóstomo, Francisco M Sánchez-Margallo, Aida Llucià-Valldeperas, Paco Bogónez-Franco, Isaac Perea-Gil, Santiago Roura, Antoni Bayes-Genis
Cardiac tissue engineering, which combines cells and biomaterials, is promising for limiting the sequelae of myocardial infarction (MI). We assessed myocardial function and scar evolution after implanting an engineered bioactive impedance graft (EBIG) in a swine MI model. The EBIG comprises a scaffold of decellularized human pericardium, green fluorescent protein-labeled porcine adipose tissue-derived progenitor cells (pATPCs), and a customized-design electrical impedance spectroscopy (EIS) monitoring system...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191018/thymosin-%C3%AE-4-improves-differentiation-and-vascularization-of-ehts
#7
Tilman Ziegler, Rabea Hinkel, Andrea Stöhr, Thomas Eschenhagen, Karl-Ludwig Laugwitz, Ferdinand le Noble, Robert David, Arne Hansen, Christian Kupatt
Induced pluripotent stem cells (iPSC) constitute a powerful tool to study cardiac physiology and represents a promising treatment strategy to tackle cardiac disease. However, iPSCs remain relatively immature after differentiation. Additionally, engineered heart tissue (EHT) has been investigated as a therapy option in preclinical disease models with promising results, although their vascularization and functionality leave room for improvement. Thymosin β4 (Tβ4) has been shown to promote the differentiation of progenitor cell lines to cardiomyocytes while it also induces angiogenic sprouting and vascular maturation...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28181589/developmental-stage-dependent-effects-of-cardiac-fibroblasts-on-function-of-stem-cell-derived-engineered-cardiac-tissues
#8
Brian Liau, Christopher P Jackman, Yanzhen Li, Nenad Bursac
We investigated whether the developmental stage of mouse cardiac fibroblasts (CFs) influences the formation and function of engineered cardiac tissues made of mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs). Engineered cardiac tissue patches were fabricated by encapsulating pure mESC-CMs, mESC-CMs + adult CFs, or mESC-CMs + fetal CFs in fibrin-based hydrogel. Tissue patches containing fetal CFs exhibited higher velocity of action potential propagation and contractile force amplitude compared to patches containing adult CFs, while pure mESC-CM patches did not form functional syncytium...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28174241/development-of-a-human-cardiac-organoid-injury-model-reveals-innate-regenerative-potential
#9
Holly K Voges, Richard J Mills, David A Elliott, Robert G Parton, Enzo R Porrello, James E Hudson
The adult human heart possesses a limited regenerative potential following an ischemic event, and undergoes a number of pathological changes in response to injury. While cardiac regeneration has been documented in zebrafish and neonatal mouse hearts, it is currently unknown whether the immature human heart is capable of undergoing complete regeneration. Combined progress in pluripotent stem cell differentiation and tissue engineering has facilitated the development of human cardiac organoids (hCO), which resemble fetal heart tissue and can be used to address this important knowledge gap...
February 7, 2017: Development
https://www.readbyqxmd.com/read/28167795/modular-assembly-of-thick-multifunctional-cardiac-patches
#10
Sharon Fleischer, Assaf Shapira, Ron Feiner, Tal Dvir
In cardiac tissue engineering cells are seeded within porous biomaterial scaffolds to create functional cardiac patches. Here, we report on a bottom-up approach to assemble a modular tissue consisting of multiple layers with distinct structures and functions. Albumin electrospun fiber scaffolds were laser-patterned to create microgrooves for engineering aligned cardiac tissues exhibiting anisotropic electrical signal propagation. Microchannels were patterned within the scaffolds and seeded with endothelial cells to form closed lumens...
February 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28156084/bone-marrow-derived-mesenchymal-stem-cell-loaded-fibrin-patches-act-as-a-reservoir-of-paracrine-factors-in-chronic-myocardial-infarction
#11
Eléonore Blondiaux, Laetitia Pidial, Gwennhael Autret, Gabriel Rahmi, Daniel Balvay, Etienne Audureau, Claire Wilhelm, Coralie L Guerin, Patrick Bruneval, Jean-Sébastien Silvestre, Philippe Menasché, Olivier Clément
The combination of mesenchymal stem cells and tissue-engineered fibrin patches improves the therapeutic efficacy of stem cells. In vivo cardiac magnetic resonance (4.7 Tesla) and ex vivo high-spatial resolution CMR were used to track the fate of human bone marrow-derived mesenchymal stem cell (BMSC) delivered on an epicardial scaffold and more specifically assess their potential intramyocardial migration. Fifty-seven nude rats underwent permanent coronary artery ligation. Two months later, those with a left ventricular ejection fraction ≤55% were randomly allocated to receive a patch loaded with human BMSC (BMSC-P, n = 10), a patch loaded with BMSCs labelled with iron oxide nanoparticles (BMSC*-P, n = 12), an acellular patch (A-P, n = 8) or to serve as sham-operated animals (SHAM, n = 7)...
February 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28154590/serum-irisin-level-can-predict-the-severity-of-coronary-artery-disease-in-patients-with-stable-angina
#12
Tolga Han Efe, Burak Açar, Ahmet Göktuğ Ertem, Kadriye Gayretli Yayla, Engin Algül, Çağrı Yayla, Sefa Ünal, Murat Bilgin, Tolga Çimen, Özgür Kirbaş, Ekrem Yeter
BACKGROUND AND OBJECTIVES: The recently discovered myokine irisin has a proposed role in adipose tissue metabolism. The aim of this study was to evaluate the relationship between serum irisin level and the coronary artery severity in patients with stable coronary artery disease (CAD). SUBJECTS AND METHODS: Sixty-three patients who underwent coronary angiography (CA) diagnosed with stable CAD and twenty-six patients with normal coronary artery (NCA) were enrolled in the study...
January 2017: Korean Circulation Journal
https://www.readbyqxmd.com/read/28149571/surgical-technique-establishing-a-pre-clinical-large-animal-model-to-test-aortic-valve-leaflet-substitute
#13
Martin Schweiger, Walter Knirsch, Niko Cesarovic, Bernard Krüger, Martin Schmiady, Thomas Frauenfelder, Laura Frese, Hitendu Dave, Simon Philipp Hoerstrup, Michael Hübler
To overcome current limitations of valve substitutes and tissue substitutes the technology of tissue engineering (TE) continues to offer new perspectives in congenital cardiac surgery. We report our experiences and results implanting a decellularized TE patch in nine sheep in orthotropic position as aortic valve leaflet substitute. Establishing the animal model, feasibility, cardiopulmonary bypass issues and operative technique are highlighted.
December 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28120758/-artificial-organs
#14
Thibaut Raguin, Agnès Dupret-Bories, Christian Debry
Research has been fighting against organ failure and shortage of donations by supplying artificial organs for many years. With the raise of new technologies, tissue engineering and regenerative medicine, many organs can benefit of an artificial equivalent: thanks to retinal implants some blind people can visualize stimuli, an artificial heart can be proposed in case of cardiac failure while awaiting for a heart transplant, artificial larynx enables laryngectomy patients to an almost normal life, while the diabetic can get a glycemic self-regulation controlled by smartphones with an artificial device...
January 2017: Médecine Sciences: M/S
https://www.readbyqxmd.com/read/28114013/wall-less-flow-phantoms-with-tortuous-vascular-geometries-design-principles-and-a-patient-specific-model-fabrication-example
#15
Chung Kit Ho, Adrian J Y Chee, Billy Y S Yiu, Anderson C O Tsang, Kwok Wing Chow, Alfred C H Yu
Flow phantoms with anatomically realistic geometry and high acoustic compatibility are valuable investigative tools in vascular ultrasound studies. Here, we present a new framework to fabricate ultrasound-compatible flow phantoms to replicate human vasculature that is tortuous, non-planar and branching in nature. This framework is based upon the integration of rapid prototyping and investment casting principles. A pedagogical walkthrough of our engineering protocol is presented in this paper using a patient-specific cerebral aneurysm model as an exemplar demonstration...
December 6, 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28111317/transplantation-of-adipose-derived-stem-cells-combined-with-neuregulin-microparticles-promotes-efficient-cardiac-repair-in-a-rat-myocardial-infarction-model
#16
Paula Díaz-Herráez, Laura Saludas, Simón Pascual-Gil, Teresa Simón-Yarza, Gloria Abizanda, Felipe Prósper, Elisa Garbayo, María José Blanco-Prieto
Tissue engineering is a promising strategy to promote heart regeneration after a myocardial infarction (MI). In this study, we investigated the reparative potential of a system that combines adipose-derived stem cells (ADSCs) with microparticles (MPs) loaded with neuregulin (NRG), named ADSC-NRG-MPs, on a rat MI model. First, cells were attached to the surface of MPs encapsulating NRG and coated with a 1:1 mixture of collagen and poly-d-lysine. One week after in vivo administration, the system favored the shift of macrophage expression from a pro-inflammatory to a regenerative phenotype...
January 19, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28103423/adipose-derived-perivascular-mesenchymal-stromal-stem-cells-promote-functional-vascular-tissue-engineering-for-cardiac-regenerative-purposes
#17
Justin Morrissette-McAlmon, Adriana Blazeski, Sarah Somers, Geran Kostecki, Leslie Tung, Warren L Grayson
Cardiac tissue engineering approaches have the potential to regenerate functional myocardium with intrinsic vascular networks. In this study, we compared the relative effects of human adipose-derived stem/stromal cells (hASCs) and human dermal fibroblasts (hDFs) in co-cultures with neonatal rat ventricular cardiomyocytes (NRVCMs) and human umbilical vein endothelial cells (HUVECs). We found that at the same ratios of NRVCM:hASC and NRVCM:hDF, the hASC co-cultures displayed shorter action potentials and maintained capture at faster pacing rates...
January 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28097523/intravascular-ultrasound-characterization-of-a-tissue-engineered-vascular-graft-in-an-ovine-model
#18
Victoria K Pepper, Elizabeth S Clark, Cameron A Best, Ekene A Onwuka, Tadahisa Sugiura, Eric D Heuer, Lilamarie E Moko, Shinka Miyamoto, Hideki Miyachi, Darren P Berman, Sharon L Cheatham, Joanne L Chisolm, Toshiharu Shinoka, Christopher K Breuer, John P Cheatham
Patients who undergo implantation of a tissue-engineered vascular graft (TEVG) for congenital cardiac anomalies are monitored with echocardiography, followed by magnetic resonance imaging or angiography when indicated. While these methods provide data regarding the lumen, minimal information regarding neotissue formation is obtained. Intravascular ultrasound (IVUS) has previously been used in a variety of conditions to evaluate the vessel wall. The purpose of this study was to evaluate the utility of IVUS for evaluation of TEVGs in our ovine model...
January 17, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28071742/core-transcription-factors-micrornas-and-small-molecules-drive-transdifferentiation-of-human-fibroblasts-towards-the-cardiac-cell-lineage
#19
Nicolas Christoforou, Syandan Chakraborty, Robert D Kirkton, Andrew F Adler, Russell C Addis, Kam W Leong
Transdifferentiation has been described as a novel method for converting human fibroblasts into induced cardiomyocyte-like cells. Such an approach can produce differentiated cells to study physiology or pathophysiology, examine drug interactions or toxicities, and engineer cardiac tissues. Here we describe the transdifferentiation of human dermal fibroblasts towards the cardiac cell lineage via the induced expression of transcription factors GATA4, TBX5, MEF2C, MYOCD, NKX2-5, and delivery of microRNAs miR-1 and miR-133a...
January 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28069694/myocardial-tissue-engineering-with-cells-derived-from-human-induced-pluripotent-stem-cells-and-a-native-like-high-resolution-3-dimensionally-printed-scaffold
#20
Ling Gao, Molly Kupfer, Jangwook Jung, Libang Yang, Patrick Zhang, Yong Sie, Quyen Tran, Visar Ajeti, Brian Freeman, Vladimir Fast, Paul Campagnola, Brenda Ogle, Jianyi Zhang
RATIONALE: Conventional three-dimensional (3D) printing techniques cannot produce structures of the size at which individual cells interact. OBJECTIVE: Here, we used multiphoton-excited, 3-dimensional printing (MPE-3DP) to generate a native-like, extracellular matrix (ECM) scaffold with submicron resolution, and then seeded the scaffold with cardiomyocytes (CMs), smooth-muscle cells (SMCs), and endothelial cells (ECs) that had been differentiated from human induced-pluripotent stem cells (iPSCs) to generate a human, iPSC-derived cardiac muscle patch (hCMP), which was subsequently evaluated in a murine model of myocardial infarction (MI)...
January 9, 2017: Circulation Research
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