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cardiac tissue engineering

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https://www.readbyqxmd.com/read/28634611/micro-and-nano-patterned-conductive-graphene-peg-hybrid-scaffolds-for-cardiac-tissue-engineering
#1
Alec S T Smith, Hyok Yoo, Hyunjung Yi, Eun Hyun Ahn, Justin H Lee, Guozheng Shao, Ekaterina Nagornyak, Michael A Laflamme, Charles E Murry, Deok-Ho Kim
A lack of electrical conductivity and structural organization in currently available biomaterial scaffolds limits their utility for generating physiologically representative models of functional cardiac tissue. Here we report on the development of scalable, graphene-functionalized topographies with anisotropic electrical conductivity for engineering the structural and functional phenotypes of macroscopic cardiac tissue constructs. Guided by anisotropic electroconductive and topographic cues, the tissue constructs displayed structural property enhancement in myofibrils and sarcomeres, and exhibited significant increases in the expression of cell-cell coupling and calcium handling proteins, as well as in action potential duration and peak calcium release...
June 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28629520/the-erythropoietin-system-protects-the-heart-upon-injury-by-cardiac-progenitor-cell-activation
#2
Maria P Zafeiriou
Erythropoietin (EPO) is a growth hormone, widely known for its role in erythropoiesis. The broad expression of erythropoietin receptor (EPOR) in adult organs suggested that EPO may also affect other cells besides late erythroid progenitors. In the embryonic heart, EPOR is expressed in all cells including the immature proliferating cardiomyocytes. In contrast to the embryonic heart in adulthood, EPOR expression is decreased and mainly detected in immature proliferating cells (i.e., resident cardiac progenitor cells) rather than in terminally differentiated cells (i...
2017: Vitamins and Hormones
https://www.readbyqxmd.com/read/28629100/biomaterials-and-cells-for-cardiac-tissue-engineering-current-choices
#3
REVIEW
Rusha Chaudhuri, Madhumitha Ramachandran, Pearl Moharil, Megha Harumalani, Amit K Jaiswal
The major purpose of cardiac tissue engineering is to engineer cells on scaffolds and use it as a substitute to infarcted cardiac cells. With an ever-increasing risk of cardiac diseases there is an increasing need to have a stable and sustainable approach to cure such ailments. This review provides a comprehensive update on the cell sources and biomaterials essential for cardiac tissue engineering, ensuring their biocompatibility under a variety of conditions. Cells can be obtained from allogenic or autologous sources...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28625174/quantification-of-cardiomyocyte-alignment-from-three-dimensional-3d-confocal-microscopy-of-engineered-tissue
#4
William J Kowalski, Fangping Yuan, Takeichiro Nakane, Hidetoshi Masumoto, Marc Dwenger, Fei Ye, Joseph P Tinney, Bradley B Keller
Biological tissues have complex, three-dimensional (3D) organizations of cells and matrix factors that provide the architecture necessary to meet morphogenic and functional demands. Disordered cell alignment is associated with congenital heart disease, cardiomyopathy, and neurodegenerative diseases and repairing or replacing these tissues using engineered constructs may improve regenerative capacity. However, optimizing cell alignment within engineered tissues requires quantitative 3D data on cell orientations and both efficient and validated processing algorithms...
June 19, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28622605/flexible-nanofilms-coated-with-aligned-piezoelectric-microfibers-preserve-the-contractility-of-cardiomyocytes
#5
P José Gouveia, S Rosa, L Ricotti, B Abecasis, H V Almeida, L Monteiro, J Nunes, F Sofia Carvalho, M Serra, S Luchkin, A Leonidovitch Kholkin, P Marques Alves, P Jorge Oliveira, R Carvalho, A Menciassi, R Pires das Neves, L Silva Ferreira
The use of engineered cardiac tissue for high-throughput drug screening/toxicology assessment remains largely unexplored. Here we propose a scaffold that mimics aspects of cardiac extracellular matrix while preserving the contractility of cardiomyocytes. The scaffold is based on a poly(caprolactone) (PCL) nanofilm with magnetic properties (MNF, standing for magnetic nanofilm) coated with a layer of piezoelectric (PIEZO) microfibers of poly(vinylidene fluoride-trifluoroethylene) (MNF+PIEZO). The nanofilm creates a flexible support for cell contraction and the aligned PIEZO microfibers deposited on top of the nanofilm creates conditions for cell alignment and electrical stimulation of the seeded cells...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28604360/chitosan-based-hydrogels-for-developing-a-small-diameter-vascular-graft-in-vitro-and-in-vivo-evaluation
#6
Audrey Aussel, Noélie Thébaud, Xavier Berard, Vincenzo Brizzi, Samantha Delmond, Reine Bareille, Robin Siadous, Chloe James, Jean Ripoche, Marlène Durand, Alexandra Montembault, Béatrice Burdin, Didier Letourneur, Nicolas L'Heureux, Laurent David, Laurence Bordenave
Vascular grafts made of synthetic polymers perform poorly in small-diameter applications (cardiac and peripheral bypass). Chitosan is a biocompatible natural polymer that can provide a novel biological scaffold for tissue engineering development. The goal of this study was to demonstrate the biocompatibility of a novel chitosan preparation in vitro and in vivo, and to assess its potential as a scaffold for vascular applications. Methods and Results. A series of experiments of increasing complexity, ranging from in vitro biocompatibility and hemocompatibility tests to in vivo studies in small and large animals (rat and sheep), was performed to provide a comprehensive analysis of chitosan hydrogels' biological properties...
June 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28590127/interwoven-aligned-conductive-nanofiber-yarn-hydrogel-composite-scaffolds-for-engineered-3d-cardiac-anisotropy
#7
Yaobin Wu, Ling Wang, Baolin Guo, Peter X Ma
Mimicking the anisotropic cardiac structure and guiding 3D cellular orientation play a critical role in designing scaffolds for cardiac tissue regeneration. Significant advances have been achieved to control cellular alignment and elongation, but it remains an ongoing challenge for engineering 3D cardiac anisotropy using these approaches. Here, we present a 3D hybrid scaffold based on aligned conductive nanofiber yarns network (NFYs-NET, composition: polycaprolactone, silk fibroin, and carbon nanotubes) within a hydrogel shell for mimicking the native cardiac tissue structure, and further demonstrate their great potential for engineering 3D cardiac anisotropy for cardiac tissue engineering...
June 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/28578271/a-gold-nanoparticle-coated-porcine-cholecyst-derived-bioscaffold-for-cardiac-tissue-engineering
#8
Reshma S Nair, Jimna Mohamed Ameer, Malcolm R Alison, Thapasimuthu V Anilkumar
Extracellular matrices of xenogeneic origin have been extensively used for biomedical applications, despite the possibility of heterogeneity in structure. Surface modification of biologically derived biomaterials using nanoparticles is an emerging strategy for improving topographical homogeneity when employing these scaffolds for sophisticated tissue engineering applications. Recently, as a tissue engineering scaffold, cholecyst derived extracellular matrix (C-ECM) has been shown to have several advantages over extracellular matrices derived from other organs such as jejunum and urinary bladder...
May 26, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28578251/cutting-edge-platforms-in-cardiac-tissue-engineering
#9
REVIEW
Sharon Fleischer, Ron Feiner, Tal Dvir
As cardiac disease takes a higher toll with each passing year, the need for new therapies to deal with the scarcity in heart donors becomes ever more pressing. Cardiac tissue engineering holds the promise of creating functional replacement tissues to repair heart tissue damage. In an attempt to bridge the gap between the lab and clinical realization, the field has made major strides. In this review, we will discuss state of the art technologies such as layer-by-layer assembly, bioprinting and bionic tissue engineering, all developed to overcome some of the major hurdles faced in the field...
June 1, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28545275/-application-of-cardiac-tissue-engineering-in-coronary-heart-disease
#10
Y J Lu, Y C Ding
No abstract text is available yet for this article.
April 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28545044/differentiation-of-spontaneously-contracting-cardiomyocytes-from-non-virally-reprogrammed-human-amniotic-fluid-stem-cells
#11
Aaron J Velasquez-Mao, Christopher J M Tsao, Madeline N Monroe, Xavier Legras, Beatrice Bissig-Choisat, Karl-Dimiter Bissig, Rodrigo Ruano, Jeffrey G Jacot
Congenital heart defects are the most common birth defect. The limiting factor in tissue engineering repair strategies is an autologous source of functional cardiomyocytes. Amniotic fluid contains an ideal cell source for prenatal harvest and use in correction of congenital heart defects. This study aims to investigate the potential of amniotic fluid-derived stem cells (AFSC) to undergo non-viral reprogramming into induced pluripotent stem cells (iPSC) followed by growth-factor-free differentiation into functional cardiomyocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28532021/nanosecond-laser-ablation-enhances-cellular-infiltration-in-a-hybrid-tissue-scaffold
#12
S Jahnavi, N Arthi, S Pallavi, C Selvaraju, G S Bhuvaneshwar, T V Kumary, R S Verma
Hybrid tissue engineered (HTE) scaffolds constituting polymeric nanofibers and biological tissues have attractive bio-mechanical properties. However, they suffer from small pore size due to dense overlapping nanofibers resulting in poor cellular infiltration. In this study, using nanosecond (ns) laser, we fabricated micro-scale features on Polycaprolactone (PCL)-Chitosan (CH) nanofiber layered bovine pericardium based Bio-Hybrid scaffold to achieve enhanced cellular adhesion and infiltration. The laser energy parameters such as fluence of 25J/cm(2), 0...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28529939/naturally-engineered-maturation-of-cardiomyocytes
#13
REVIEW
Gaetano J Scuderi, Jonathan Butcher
Ischemic heart disease remains one of the most prominent causes of mortalities worldwide with heart transplantation being the gold-standard treatment option. However, due to the major limitations associated with heart transplants, such as an inadequate supply and heart rejection, there remains a significant clinical need for a viable cardiac regenerative therapy to restore native myocardial function. Over the course of the previous several decades, researchers have made prominent advances in the field of cardiac regeneration with the creation of in vitro human pluripotent stem cell-derived cardiomyocyte tissue engineered constructs...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28528953/composite-poly-lactic-acid-chitosan-nanofibrous-scaffolds-for-cardiac-tissue-engineering
#14
Yaowen Liu, Shuyao Wang, Rong Zhang
Fibrous scaffolds with different ratios of poly (lactic acid) (PLA) and chitosan were fabricated by conventional electrospinning. After crosslinking by the glutaraldehyde vapor, the structure, mechanical properties, hydrophilicity, and in-fiber chemical interactions of the scaffolds were investigated. We found that the fiber diameter decreased with the concentration of chitosan, while mechanical properties and hydrophilicity improved. In addition, we found that scaffolds with aligned fibers have higher mechanical strength and biocompatibility than scaffolds with randomly oriented fibers...
May 18, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28518082/maturation-of-human-stem-cell-derived-cardiomyocytes-in-biowires-using-electrical-stimulation
#15
Xuetao Sun, Sara S Nunes
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have been a promising cell source and have thus encouraged the investigation of their potential applications in cardiac research, including drug discovery, disease modeling, tissue engineering, and regenerative medicine. However, cells produced by existing protocols display a range of immaturity compared with native adult ventricular cardiomyocytes. Many efforts have been made to mature hPSC-CMs, with only moderate maturation attained thus far. Therefore, an engineered system, called biowire, has been devised by providing both physical and electrical cues to lead hPSC-CMs to a more mature state in vitro...
May 6, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513656/restoring-the-biophysical-properties-of-decellularized-patches-through-recellularization
#16
Gigi Chi Ting Au-Yeung, Udi Sarig, Hadar Sarig, Hanumakumar Bogireddi, Tomer Bronshtein, Limor Baruch, Avihai Spizzichino, Jacob Bortman, Boey Yin Chiang Freddy, Marcelle Machluf, Subbu S Venkatraman
Various extracellular matrix (ECM) scaffolds, isolated through decellularization, were suggested as ideal biomimetic materials for 'Functional tissue engineering' (FTE). The decellularization process comprises a compromise between damaging and preserving the ultrastructure and composition of ECM-previously shown to affect cell survival, proliferation, migration, organization, differentiation and maturation. Inversely, the effects of cells on the ECM constructs' biophysical properties, under physiological-like conditions, remain still largely unknown...
May 30, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28509913/optimization-of-a-polydopamine-pd-based-coating-method-and-polydimethylsiloxane-pdms-substrates-for-improved-mouse-embryonic-stem-cell-esc-pluripotency-maintenance-and-cardiac-differentiation
#17
Jiayin Fu, Yon Jin Chuah, Wee Tong Ang, Nan Zheng, Dong-An Wang
Myocardiocyte derived from pluripotent stem cells, such as induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), is a promising cell source for cardiac tissue engineering. Combined with microfluidic technologies, a heart-on-a-chip is very likely to be developed and function as a platform for high throughput drug screening. Polydimethylsiloxane (PDMS) silicone elastomer is a widely-used biomaterial for the investigation of cell-substrate interactions and biochip fabrication. However, the intrinsic PDMS surface hydrophobicity inhibits cell adhesion on the PDMS surface, and PDMS surface modification is required for effective cell adhesion...
May 30, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28498548/engineered-3d-cardiac-fibrotic-tissue-to-study-fibrotic-remodeling
#18
Amir Hossein Sadeghi, Su Ryon Shin, Janine C Deddens, Giuseppe Fratta, Serena Mandla, Iman K Yazdi, Gyan Prakash, Silvia Antona, Danilo Demarchi, Marc P Buijsrogge, Joost P G Sluijter, Jesper Hjortnaes, Ali Khademhosseini
Activation of cardiac fibroblasts into myofibroblasts is considered to play an essential role in cardiac remodeling and fibrosis. A limiting factor in studying this process is the spontaneous activation of cardiac fibroblasts when cultured on two-dimensional (2D) culture plates. In this study, a simplified three-dimensional (3D) hydrogel platform of contractile cardiac tissue, stimulated by transforming growth factor-β1 (TGF-β1), is presented to recapitulate a fibrogenic microenvironment. It is hypothesized that the quiescent state of cardiac fibroblasts can be maintained by mimicking the mechanical stiffness of native heart tissue...
June 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28498093/cardiac-tissue-engineering-from-matrix-design-to-the-engineering-of-bionic-hearts
#19
Sharon Fleischer, Ron Feiner, Tal Dvir
The field of cardiac tissue engineering aims at replacing the scar tissue created after a patient has suffered from a myocardial infarction. Various technologies have been developed toward fabricating a functional engineered tissue that closely resembles that of the native heart. While the field continues to grow and techniques for better tissue fabrication continue to emerge, several hurdles still remain to be overcome. In this review we will focus on several key advances and recent technologies developed in the field, including biomimicking the natural extracellular matrix structure and enhancing the transfer of the electrical signal...
April 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28498040/pivotal-role-of-non-cardiomyocytes-in-electromechanical-and-therapeutic-potential-of-induced-pluripotent-stem-cell-derived-engineered-cardiac-tissue
#20
Hiroko Iseoka, Shigeru Miyagawa, Satsuki Fukushima, Atsuhiro Saito, Shigeo Masuda, Shin Yajima, Emiko Ito, Nagako Sougawa, Maki Takeda, Akima Harada, Jong-Kook Lee, Yoshiki Sawa
Although engineered cardiac tissues (ECTs) derived from induced pluripotent stem cells (iPSCs) are promising for myocardial regenerative therapy, the appropriate ratio of cardiomyocytes to non-cardiomyocytes is not fully understood. Here, we determined whether ECT-cell content is a key determinant of its structure/function, thereby affecting ECT therapeutic potential for advanced heart failure. Scaffold-free ECTs containing different ratios (25%, 50%, 70%, or 90%) of iPSC-derived cardiomyocytes were generated by magnetic-activated cell sorting by using cardiac-specific markers...
June 13, 2017: Tissue Engineering. Part A
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