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Diabetes mirna

Alieh Gholaminejad, Hossein Abdul Tehrani, Mohammad Gholami Fesharaki
AIMS: The aim was to perform a meta-analysis on the miRNA expression profiling studies in diabetic nephropathy (DN) to identify candidate diagnostic biomarkers. METHODS: A comprehensive literature search was done in several databases and 53 DN miRNA expression studies were selected. To identify significant DN-miR meta-signatures, two meta-analysis methods were employed: vote-counting strategy and the robust rank aggregation method. The targets of DN-miRs were obtained and a gene set enrichment analysis was carried out to identify the pathways most strongly affected by dysregulation of these miRNAs...
July 17, 2018: Journal of Nephrology
Anindita Das, Arun Samidurai, Fadi N Salloum
After being long considered as "junk" in the human genome, non-coding RNAs (ncRNAs) currently represent one of the newest frontiers in cardiovascular disease (CVD) since they have emerged in recent years as potential therapeutic targets. Different types of ncRNAs exist, including small ncRNAs that have fewer than 200 nucleotides, which are mostly known as microRNAs (miRNAs), and long ncRNAs that have more than 200 nucleotides. Recent discoveries on the role of ncRNAs in epigenetic and transcriptional regulation, atherosclerosis, myocardial ischemia/reperfusion (I/R) injury and infarction (MI), adverse cardiac remodeling and hypertrophy, insulin resistance, and diabetic cardiomyopathy prompted vast interest in exploring candidate ncRNAs for utilization as potential therapeutic targets and/or diagnostic/prognostic biomarkers in CVDs...
2018: Frontiers in Cardiovascular Medicine
Aurora Mazzeo, Elena Beltramo, Tatiana Lopatina, Chiara Gai, Marina Trento, Massimo Porta
Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing their detachment and migration, and stimulating angiogenesis in vitro. The purpose of this work was the molecular and functional characterization of EVs derived from diabetic subjects with or without diabetic retinopathy, compared with healthy controls...
July 3, 2018: Experimental Eye Research
Hongmei Lang, Yang Xiang, Ning Lin, Zhihua Ai, Zhiqing You, Jie Xiao, Dan Liu, Yongjian Yang
BACKGROUND/AIMS: MicroRNAs (miRNAs) are a novel class of small RNAs that participate in a variety of biological processes. Although miRNAs have been linked to insulin synthesis and glucose homeostasis, their role in the targeting of mitochondrial uncoupling protein 2 (UCP2), a negative modulator of insulin secretion, remains unclear. METHODS: miRNA levels were determined by real-time quantitative PCR analysis using TaqMan probes, and insulin secretion from isolated islets was quantified by ELISA...
July 13, 2018: Cellular Physiology and Biochemistry
Munekazu Yamakuchi, Teruto Hashiguchi
Endothelial cells (ECs) form monolayers and line the interior surfaces of blood vessels in the entire body. In most mammalian systems, the capacity of endothelial cells to divide is limited and endothelial cells are prone to be senescent. Aging of ECs and resultant endothelial dysfunction lead to a variety of vascular diseases such as atherosclerosis, diabetes mellites, hypertension, and ischemic injury. However, the mechanism by which ECs get old and become senescent and the impact of endothelial senescence on the vascular function are not fully understood...
July 10, 2018: Journal of Clinical Medicine
Mohammad R Irhimeh, Mohamed Hamed, Daniel Barthelmes, Yvonne Gladbach, Volkhard Helms, Weiyong Shen, Mark C Gillies
Endothelial progenitor cells (EPCs) are a group of rare cells that play an important role in the repair of injured vascular endothelial cells and assist in reperfusion of ischemic tissue. Decreased production and/or loss of function of EPCs are associated with diabetic vasculopathy. The molecular mechanisms by which diabetes impairs EPCs remain unclear. We conducted microarray experiments followed by integrative regulatory analysis on cells isolated from Akita diabetic mice (18-weeks after onset of diabetes) and age-matched non-diabetic controls...
2018: PloS One
Cristina Beltrami, Kate Simpson, Mark Jesky, Alexa Wonnacott, Christopher Carrington, Peter Holmans, Lucy Newbury, Robert Jenkins, Thomas Ashdown, Colin Dayan, Simon Satchell, Peter Corish, Paul Cockwell, Donald Fraser, Timothy Bowen
Effective diabetic kidney disease (DKD) biomarkers remain elusive, and urinary microRNAs (miRNAs) represent a potential source of novel non-invasive disease sentinels. We profiled 754 miRNAs in pooled urine samples from DKD patients (n = 20), detecting significantly increased miR-126, miR-155, and miR-29b compared to controls (n = 20). These results were confirmed in an independent cohort of 89 DKD patients, 62 diabetic patients without DKD and 41 controls: miR-126 (2.8-fold increase; P 0 .0001), miR-155 (1...
July 5, 2018: American Journal of Pathology
Hamid Ghaedi, Ali Zare, Mir Davood Omrani, Amir Hossein Doustimotlagh, Reza Meshkani, Sadegh Alipoor, Behnam Alipoor
PURPOSE: Long-noncoding RNAs (lncRNAs) have been reported to regulate glucose homeostasis and insulin synthesis and secretion. However, the association of genetic variants of lncRNAs and type 2 diabetes (T2D) has not been evaluated. Therefore, in the present study we investigated the association between H19 rs217727 and H19 rs3741219 variants and MEG3 rs7158663 polymorphism with T2D susceptibility. MATERIALS AND METHODS: The study population consists of 969 subjects including 496 T2D patients and 473 non-diabetic age and sex-matched controls...
July 4, 2018: Gene
André Gomes, Inês V da Silva, Cecília M P Rodrigues, Rui E Castro, Graça Soveral
Aquaporins (AQPs) are membrane channels widely distributed in human tissues. AQPs are essential for water and energy homeostasis being involved in a broad range of pathophysiological processes such as edema, brain injury, glaucoma, nephrogenic diabetes insipidus, salivary and lacrimal gland dysfunction, cancer, obesity and related metabolic complications. Compelling evidence indicates that AQPs are targets for therapeutic intervention with potential broad application. Nevertheless, efficient AQP modulators have been difficult to find due to either lack of selectivity and stability, or associated toxicity that hamper in vivo studies...
2018: Frontiers in Chemistry
Mahrima Parvin, Farhan Jahan, Pankaj Kumar Sarkar, Zakir Hossain Howlader, A H M Nurun Nabi, Md Ismail Hosen
Bangladesh has the second largest number of adults with diabetes in South Asia. Compelling evidence suggest that miRNAs contribute to the etiology of Type 2 diabetes mellitus (T2DM) by regulating many aspects of glucose homeostasis. Hence, we hypothesized that genetic polymorphisms in the diabetes-related miRNA target-binding sites could be associated with the risk of T2DM in Bangladesh. The reference Single nucleotide polymorphism (SNP) data from the Insulin Receptor (INSR) gene were downloaded from the ENSEMBL genome browser release 88 and further analyzed in silico for identifying SNPs with deleterious effect and clinical relationships...
July 4, 2018: Biochemical Genetics
Guo Wei, Shao Yi, Dai Yong, Liu Shaozhuang, Zhang Guangyong, Hu Sanyuan
BACKGROUND: Duodenal-jejunal bypass (DJB) surgery can improve type 2 diabetes (T2D) dramatically. Accumulating evidence implicates deficiency of hepatic adiponectin signaling as a contributor to gluconeogenesis disorders, and some microRNAs (miRNAs) regulate adiponectin receptors (AdipoR1, AdipoR2). We investigated the effects of DJB on hepatic gluconeogenesis, lipid metabolism, and inflammation as well as the effects of miRNA-320 (AdipoR1-targeting miRNA) on DJB-induced T2D amelioration...
March 15, 2018: Surgery for Obesity and related Diseases: Official Journal of the American Society for Bariatric Surgery
Li-Li Sun, Wen-Dong Li, Feng-Rui Lei, Xiao-Qiang Li
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level via either the degradation or translational repression of a target mRNA. They play an irreplaceable role in angiogenesis by regulating the proliferation, differentiation, apoptosis, migration and tube formation of angiogenesis-related cells, which are indispensable for multitudinous physiological and pathological processes, especially for the occurrence and development of vascular diseases. Imbalance between the regulation of miRNAs and angiogenesis may cause many diseases such as cancer, cardiovascular disease, aneurysm, Kawasaki disease, aortic dissection, phlebothrombosis and diabetic microvascular complication...
June 29, 2018: Journal of Cellular and Molecular Medicine
Saikat Dewanjee, Niloy Bhattacharjee
Diabetic nephropathy (DN) is one of the most prevalent lethal complications of diabetes that leads to end stage renal disease. Although several clinical approaches exist to attenuate DN, there is not curative treatment to date. DN is complicated, as it involves several simultaneous molecular pathways. Some natural and synthetic molecules have been reported to inhibit some specific pathogenic signal transduction in DN. However, the complications of DN still remain uncontrolled. MicroRNAs (miRNAs) are a class of non-coding RNAs that can bind to the 3'UTR of their target mRNAs to participate in epigenetic regulation of their downstream signalling molecules...
June 22, 2018: Biochemical Pharmacology
Jing Li, Ziqiang Fu, Hua Jiang, Liping Chen, Xian Wu, Hongjuan Ding, Yankai Xia, Xinru Wang, Qiuqin Tang, Wei Wu
STUDY QUESTION: What is the role of insulin-like growth factor 2 (IGF2)-derived miR-483-3p in macrosomia? SUMMARY ANSWER: IGF2-derived intronic miR-483-3p is overexpressed in macrosomia placentas, and miR-483-3p prompts HTR-8/SVneo extravillous trophoblast cell line proliferation through down-regulation of its target RB1 inducible coiled-coil 1 (RB1CC1). WHAT IS KNOWN ALREADY: Macrosomia is a common pregnancy-associated disease and causes a number of adverse maternal and perinatal outcomes...
June 25, 2018: Molecular Human Reproduction
Geeta Shroff, Rhea Shroff, Rakesh Gupta
Diabetes mellitus occurs either due to an autoimmune destruction of β cells (Type 1) or resistance to insulin effects (Type 2). Diverse conventional medications are used for treatment of diabetes, which is associated with long term complications such as kidney failure, blindness, and stroke. We recently showed the potential of human embryonic stem cells (hESCs) in 95 patients with type 2 diabetes. In the present study, we use the microarray and miRNA studies to prove why hESCs are effective in diabetes. Three samples of hESCs were cultured and microarray technology was used for the analysis of diabetic pathways...
2018: American Journal of Stem Cells
Jian Wu, Jibo Liu, Yaqin Ding, Mingying Zhu, Kan Lu, Jing Zhou, Xin Xie, Yi Xu, Xiaoyu Shen, Yiyue Chen, Xiaohong Shao, Chunling Zhu
Emerging evidence has shown that microRNAs (miRNAs) play a mediatory role in the pathogenesis of diabetic nephropathy (DN), but the function of the involved miRNAs is still incomplete. Here, we found that miR-455-3p was down-regulated in the human mesangial cells (HMC) and human proximal tubule epithelial cells (HK-2) stimulated with high glucose (HG) or transforming growth factor beta 1 (TGF-β1). Rho-associated coiled coil-containing protein kinase 2 (ROCK2) was identified as a directed target of miR-455-3p...
June 19, 2018: Biochemical and Biophysical Research Communications
Zahra Hatefi, Goljahan Soltani, Sharifeh Khosravi, Mohammad Kazemi, Ahmad Reza Salehi, Rasoul Salehi
Background: The relationship between dyslipidemia and type 2 diabetes mellitus (T2DM) has been frequently reported. Lipoprotein lipase (LPL) is considered to be an effective gene in regulating lipid profile. MicroRNAs (miRNAs) are small noncoding RNAs involved in posttranscriptional regulation of gene expression. In the present study, we have evaluated rs13702 (C/T) polymorphism located in miRNA-410 binding site of LPL gene in subset of Iranian T2DM patients and their normal counterparts...
2018: Advanced Biomedical Research
Oğuzhan Fatih Baltacı, Şeyma Çolakoğlu, Gökçe Güllü Amuran, Neslihan Aydın, Mehmet Sarğın, Arzu Karabay, Temel Yılmaz, Ergül Berber
Background/aim: MODY3 associated with HNF1A is the most common form of MODY and is clinically misdiagnosed as type 1 diabetes due to similar clinical symptoms. This study aimed to analyze the role of HNF1A-regulated miRNAs as a biomarker in the diagnosis of MODY3. Materials and methods: MIN6 cells were transfected with the HNF1A cDNA expression vector for overexpression or with siRNA specific to HNF1A to silence its expression. The HNF1A-regulated miRNAs were determined by RNA-Seq of the total RNA extract. Expressions of the candidate miRNAs in blood samples of MODY3, type 1 diabetes, and type 2 diabetes patients and in healthy subjects were compared statistically by Mann-Whitney U tests...
June 14, 2018: Turkish Journal of Medical Sciences
Songcheng Yu, Yan Wang, Xing Li, Zhenxing Mao, Fei Yu, Ling Wang, Yue Ba, Chongjian Wang, Wenjie Li
As the major vitamin D binding protein (DBP), the group-specific component (GC) plays an important role in the bioactivity of vitamin D. Abnormal expression of GC gene may be associated with vitamin D related disease, type 2 diabetes mellitus (T2DM). DNA methylation is an important regulator of gene expression. It has been reported that methylation in 3' untranslated region played a role in regulation of protein expression via interaction with miRNA. This study hypothesized that DNA methylation of 3' near region of GC gene (3'GC) might be associated with T2DM...
June 2018: Nutrition Research
Qian Sun, Qianlei Yang, Hui Xu, Junchao Xue, Chao Chen, Xingfen Yang, Xiaohua Gao, Qizhan Liu
Chronic exposure to arsenic, a potent environmental oxidative stressor, is associated with the incidence of diabetes. However, the mechanisms for arsenite-induced reduction of insulin remain largely unclear. After CD1 mice were treated with 20 or 40 ppm arsenite in the drinking water for 12 months, the mice showed reduced fasting insulin levels, a depression in glucose clearance, and lower insulin content in the pancreas. The levels of glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells isolated from arsenite-exposed mice were low compared to those for control mice...
June 14, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
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