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Diabetes mirna

Xiaoping Chen, Lei Zhao, Yanwei Xing, Bo Lin
BACKGROUND: Several miRNAs including miR-21 have emerged as important regulators in the development of diabetic nephropathy (DN). However, the molecular mechanism of miR-21 underlying DN pathogenesis remains to be further discussed. METHODS: Streptozotocin (STZ)-induced DN rats and high glucose (HG)-induced podocytes were used as the in vivo and in vitro models of DN. miR-21 level was detected by qRT-PCR assay. Inflammatory cytokine levels were assessed by ELISA...
September 10, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Rosa Jiménez-Lucena, Oriol Alberto Rangel-Zúñiga, Juan Francisco Alcalá-Díaz, Javier López-Moreno, Irene Roncero-Ramos, Helena Molina-Abril, Elena Maria Yubero-Serrano, Javier Caballero-Villarraso, Javier Delgado-Lista, Justo Pastor Castaño, Jose Maria Ordovás, Pablo Pérez-Martinez, Antonio Camargo, José López-Miranda
Circulating microRNAs (miRNAs) have been proposed as type 2 diabetes biomarkers, and they may be a more sensitive way to predict development of the disease than the currently used tools. Our aim was to identify whether circulating miRNAs, added to clinical and biochemical markers, yielded better potential for predicting type 2 diabetes. The study included 462 non-diabetic patients at baseline in the CORDIOPREV study. After a median follow-up of 60 months, 107 of them developed type 2 diabetes. Plasma levels of 24 miRNAs were measured at baseline by qRT-PCR, and other strong biomarkers to predict diabetes were determined...
September 7, 2018: Molecular Therapy. Nucleic Acids
Zahra Azhir, Fariba Dehghanian, Zohreh Hojati
Type 1 diabetes (T1D) is an autoimmune disorder which is characterized by autoimmune attack on β cells of pancreas and lack of insulin. The involvement of microRNAs (miRNAs) in the development of immune system and their differential expression in various autoimmune diseases including T1D have been well established. In this study, the association between expression levels of miR-20a, miR-326 and T1D were evaluated. The expression levels of miR-20a and miR-326 were measured in the PBMCs of 21 T1D patients and 16 healthy controls using qPCR method...
September 7, 2018: Molecular Biology Reports
Fatimah T AlRashidi, Kathleen M Gillespie
PURPOSE OF REVIEW: Islet transplantation, an important approach to achieve insulin independence for individuals with type 1 diabetes, is limited by the lack of accurate biomarkers to track beta-cell death post islet infusion. In this review, we will discuss existing and recently described biomarkers. RECENT FINDINGS: As beta cells are killed by the immune system, fragments of beta cell-specific cell-free DNA and proteins are released into the periphery. Several different strategies to identify these fragments have been described...
September 5, 2018: Current Diabetes Reports
Beibei Dai, Huaping Li, Jiahui Fan, Yanru Zhao, Zhongwei Yin, Xiang Nie, Dao Wen Wang, Chen Chen
BACKGROUND: Diabetes is a leading cause of mortality and morbidity across the world. Over 50% of deaths among diabetic patients are caused by cardiovascular diseases. Cardiac diastolic dysfunction is one of the key early signs of diabetic cardiomyopathy, which often occurs before systolic dysfunction. However, no drug is currently licensed for its treatment. METHODS: Type 9 adeno-associated virus combined with cardiac Troponin T promoter were employed to manipulate miR-21 expression in the leptin receptor-deficient (db/db) mice...
September 4, 2018: Cardiovascular Diabetology
Fanny Langlet, Marcel Tarbier, Rebecca A Haeusler, Stefania Camastra, Eleuterio Ferrannini, Marc R Friedländer, Domenico Accili
OBJECTIVES: Hepatic insulin resistance is a hallmark of type 2 diabetes and obesity. Insulin receptor signaling through AKT and FOXO has important metabolic effects that have traditionally been ascribed to regulation of gene expression. However, whether all the metabolic effects of FOXO arise from its regulation of protein-encoding mRNAs is unknown. METHODS: To address this question, we obtained expression profiles of FOXO-regulated murine hepatic microRNAs (miRNAs) during fasting and refeeding using mice lacking Foxo1, 3a, and 4 in liver (L-Foxo1,3a, 4)...
August 11, 2018: Molecular Metabolism
Taís S Assmann, Mariana Recamonde-Mendoza, Aline R Costa, Márcia Puñales, Balduíno Tschiedel, Luís H Canani, Andrea C Bauer, Daisy Crispim
AIMS: The aim of this study was to investigate a miRNA expression profile in type 1 diabetes mellitus (T1DM) patients with DKD (cases) or without this complication (controls). METHODS: Expression of 48 miRNAs was screened in plasma of 58 T1DM patients (23 controls, 18 with moderate DKD, and 17 with severe DKD) using TaqMan Low Density Array cards (Thermo Fisher Scientific). Then, five of the dysregulated miRNAs were selected for validation in an independent sample of 10 T1DM controls and 19 patients with DKD (10 with moderate DKD and 9 with severe DKD), using RT-qPCR...
August 30, 2018: Acta Diabetologica
P Prabu, S Rome, C Sathishkumar, C Gastebois, E Meugnier, V Mohan, M Balasubramanyam
AIMS: MicroRNAs (miRNAs) from extracellular vesicles (EVs) have been proposed as promising biomarkers for a number of diseases. In this study, their potential as urine-based biomarkers of diabetic nephropathy (DN) was assessed. METHODS: MiRNAs were profiled in urinary EVs from 160 fasting subjects with normal glucose tolerance (NGT) and in T2DM patients with either microalbumininuria (MIC) or macroalbuminuria (MAC). RESULTS: A total of 73 miRNAs detected in urinary EVs (NGT) were predicted to target important functions for kidney homoeostasis, thereby validating their use as indicators of kidney dysfunction...
August 27, 2018: Diabetes & Metabolism
Leslie R Sedgeman, Carine Beysen, Ryan M Allen, Marisol A Ramirez Solano, Scott M Turner, Kasey C Vickers
Colesevelam is a bile acid sequestrant approved to treat both hyperlipidemia and type 2 diabetes, but the mechanism for its glucose lowering effects is not fully understood. The aim of this study was to investigate the role of hepatic microRNA's as regulators of metabolic disease and to investigate the link between the cholesterol and glucose lowering effects of colesevelam. To quantify the impact of colesevelam treatment in rodent models of diabetes, metabolic studies were performed in Zucker Diabetic Fatty (ZDF) rats and db/db mice...
August 30, 2018: American Journal of Physiology. Gastrointestinal and Liver Physiology
Chandra K Bhattacharjee, Suman K Paine, Jagadish Mahanta, Sawjib Borphukan, Prasanta K Borah
No abstract text is available yet for this article.
August 26, 2018: Journal of Diabetes
Asif Nadeem, Muhammad Rizwan Ashraf, Maryam Javed, Tanveer Hussain, Muhammad Salman Tariq, Masroor Ellahi Babar
In 1993 miRNAs were discovered during a research on Caenorhabditis elegans conducted by Victor Ambros and Gary Ruvkun. The gene lin-4 that played important role in development in C. elgans was observed not encoding any protein but a very small RNA molecule of just 22 nucleotides. Main objective of this review is to highlight the significance of miRNAs in regulating the expression of many genes, which are either directly or indirectly involved in many diseases. One of the major causes of illness and death in developed countries of the world is cardiovascular disease...
September 2018: Pakistan Journal of Pharmaceutical Sciences
Denizhan Ozdemir, Mark W Feinberg
Diabetic wound healing is an incompletely understood pathophysiological state. It comprises a range of potentially devastating and common complications of diabetes mellitus (DM) leading to intractable infections, lower extremity amputations, and associated cardiovascular morbidity and mortality. MicroRNAs (miRNAs) have emerged as important regulators in various physiological processes in health and disease through their ability to fine-tune cellular responses. Herein, we summarize the versatile roles of miRNAs implicated in diabetic wound healing in key stages including inflammation, angiogenesis, re-epithelialization, and remodeling...
August 8, 2018: Trends in Cardiovascular Medicine
Jing Li, Mei Luo, Minghua Hu, An-Yuan Guo, Xiangliang Yang, Qiong Zhang, Yanhong Zhu
Diabetes is generally regarded as a metabolic disorder disease caused by various reasons, including pancreas islet injury and lipid metabolism disorders. The aqueous extract of Cyclocarya paliurus leaves (CPAE) was reported to be anti-diabetic. However, the possible molecular mechanisms have not been investigated. To elucidate the anti-diabetic effects of CPAE and the underlying potential mechanisms, we performed transcriptome profiling (RNA-Seq and miRNA-Seq) on the pancreas and liver from non-diabetic, diabetic and diabetic-CPAE rats...
2018: Frontiers in Pharmacology
Qiqing Huang, Weiyan You, Yating Li, Yi Sun, Yuncai Zhou, Yan Zhang, Dechen Liu, Shanshan Zhan, Yunxia Zhu, Xiao Han
Inhibition of microRNAs (miRNAs) essential for pancreatic β-cell biology (e.g., miR-375 ) results in β-cell failure and diabetes in rodent models. Whether the downregulation of miRNAs in pancreatic islets is involved in the development of human type 2 diabetes remains unclear. Here, utilizing a miRNA microarray, we identified a set of miRNAs that were differentially expressed in healthy human islets under glucolipotoxic conditions. A downregulated miRNA, miR-299-5p , was preferentially studied since its inhibition caused dramatic β-cell dysfunction and apoptosis...
August 21, 2018: Diabetes
Zhiqiang Sun, Yali Ma, Fang Chen, Shiying Wang, Baoping Chen, Jun Shi
Transforming growth factor-β1 (TGF-β1)-induced epithelial to mesenchymal transition (EMT) and renal fibrosis plays critical role in the development and progression of diabetic nephropathy (DN). Our study aimed to determine the detailed roles of miR-133b & miR-199b on TGF-β1-induced EMT & renal fibrosis in DN and its underlying mechanism. The expressions of miR-133b & miR-199b in OLETF rats, LETO rats & TGF-β1-treated human proximal tubule cell line (HK-2) were examined by qRT-PCR. Inhibition of miR-133b or miR-199b was realized in cells by transfection of lentivirus containing miR-133b inhibit or miR-199b inhibitor...
August 17, 2018: European Journal of Pharmacology
Di Che, Jiawen Li, Lanyan Fu, Lei Pi, Xing Rong, Yanfei Wang, Yufen Xu, Ping Huang, Maoping Chu, Xiaoqiong Gu
Background: Kawasaki disease (KD) mainly manifests as excessive inflammation and vascular endothelial cell injury. This disease generally occurs in children younger than 5 years of age and is more severe in children younger than 12 months. KD affects males and females at a ratio of 1.5:1. Polymorphisms of the rs1625579 locus in the miR-13 gene are associated with schizophrenia susceptibility, and high glucose-induced upregulation of miR-137 in vascular endothelial cells promotes monocyte chemotaxis and inflammatory cytokine secretion in gestational diabetes mellitus...
2018: Infection and Drug Resistance
Xiaohui Zhang, Yuhong Yang, Zhaohui Feng
High glucose (HG)-induced apoptosis of retinal ganglion cells (RGCs) contributes to the pathogenesis of diabetic retinopathy, which is one of the most common and severe complications of diabetes mellitus. Accumulating evidence has documented that microRNAs (miRNAs) play an important role in the pathogenesis of diabetic retinopathy. However, the role of miRNAs in regulating HG-induced apoptosis of RGCs remains largely unknown. Various studies have suggested that miR-495 is an important regulator of cell apoptosis and survival...
October 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Corrie L Gallant-Behm, Joseph Piper, Brent A Dickinson, Christina M Dalby, Linda A Pestano, Aimee L Jackson
There is a strong unmet need for new therapeutics to accelerate wound healing across both chronic and acute indications. It is well established that local tissue hypoxia, vascular insufficiency and/or insufficient angiogenesis contribute to inadequate wound repair in the context of diabetic foot ulcers as well as to other chronic wounds such as venous stasis and pressure ulcers. microRNA-92a-3p (miR-92a) is a potent anti-angiogenic miRNA whose inhibition has led to increases in angiogenesis in multiple organ systems, resulting in an improvement in function following myocardial infarction, limb ischemia, vascular injury and bone fracture...
August 17, 2018: Wound Repair and Regeneration
Li Chen, Haiyun Qian, Junli Xue, Juan Zhang, Hao Chen
An increasing number of microRNAs (miRNAs/miRs) are reported to have important roles in diabetes. Glucose‑stimulated insulin secretion and pancreatic β cell proliferation are essential in the control of metabolic disorder, however, the underlying molecular mechanisms remain unclear. The present study investigated the function of miR‑152 in diabetes. The results of reverse transcription‑quantitative polymerase chain reaction demonstrated that miR‑152 levels in the blood were markedly reduced in patients with diabetes compared with nondiabetic controls...
August 8, 2018: Molecular Medicine Reports
Tandi E Matsha, Andre P Kengne, Stanton Hector, Desiree L Mbu, Yandiswa Y Yako, Rajiv T Erasmus
Early identification of individuals with elevated risk of developing diabetes mellitus, followed by the implementation of effective prevention interventions can delay the onset of the disease and related complications. In this regard, recent studies have shown that miRNAs are useful as early markers of certain disease types, including diabetes. We used high throughput sequencing to assess miRNA expression profiles from whole blood of 12 individuals with screen-detected diabetes, 12 with prediabetes and 12 with normal glucose tolerance, matched for age, blood pressure, smoking and body mass index...
July 17, 2018: Oncotarget
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