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MicroRNAs and early detection

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https://www.readbyqxmd.com/read/30240965/a-facile-dna-strand-displacement-reaction-sensing-strategy-of-electrochemical-biosensor-based-on-n-carboxymethyl-chitosan-molybdenum-carbide-nanocomposite-for-microrna-21-detection
#1
Liang Tian, Jinxu Qi, Xiangyu Ma, Xuejiao Wang, Chen Yao, Wei Song, Yihong Wang
Herein, we report a facile enzyme-free microRNA (miRNA) target-triggered strand displacement reaction (SDR) amplification strategy with ferrocene (Fc) as a signal molecule to fabricate a two-dimensional electroactive molybdenum carbide (Mo2 C)-based biosensor. In the presence of miRNA-21, SDR was initiated and many hairpin DNA1 (HDNA1) and hairpin DNA2 (HDNA2) duplexes, which could be captured by probe DNA leading the Fc-modified HDNA2 close to the electrode surface, were produced continuously. MiRNA-21 could be detected by monitoring the redox signal of Fc...
September 13, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30238748/ultrasensitive-detection-of-serum-microrna-using-branched-dna-based-sers-platform-combining-simultaneous-detection-of-%C3%AE-fetoprotein-for-early-diagnosis-of-liver-cancer
#2
Linxiu Cheng, Zhikun Zhang, Duo Zuo, Wenfeng Zhu, Jie Zhang, Qingdao Zeng, Dayong Yang, Min Li, Yuliang Zhao
We provided an ultrasensitive sensing strategy for microRNA detection by firstly employing branched DNA. With the aid of micro-contact printing, we realized the multiplex sensing of different kinds of liver cancer biomarkers: microRNA and protein, simultaneously. Delicately designed branched-DNA included multiple complementary sticky-ends as probe to microRNA capture and the double-stranded rigid branched core to increase active sticky-ends distance and expose more DNA probes for sensitivity. The branched-DNA enables 2 orders of magnitude increase in sensitivity for microRNA detection over single-stranded DNA...
September 21, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30237481/mir-873-5p-acts-as-an-epigenetic-regulator-in-early-stages-of-liver-fibrosis-and-cirrhosis
#3
David Fernández-Ramos, Pablo Fernández-Tussy, Fernando Lopitz-Otsoa, Virginia Gutiérrez-de-Juan, Nicolás Navasa, Lucía Barbier-Torres, Imanol Zubiete-Franco, Jorge Simón, Agustín F Fernández, Ander Arbelaiz, Ana M Aransay, José Luis Lavín, Naiara Beraza, María J Perugorria, Jesus M Banales, Erica Villa, Mario F Fraga, Juan Anguita, Matias A Avila, Carmen Berasain, Paula Iruzibieta, Javier Crespo, Shelly C Lu, Marta Varela-Rey, José M Mato, Teresa C Delgado, María L Martínez-Chantar
Glycine N-methyltransferase (GNMT) is the most abundant methyltransferase in the liver and a master regulator of the transmethylation flux. GNMT downregulation leads to loss of liver function progressing to fibrosis, cirrhosis, and hepatocellular carcinoma. Moreover, GNMT deficiency aggravates cholestasis-induced fibrogenesis. To date, little is known about the mechanisms underlying downregulation of GNMT levels in hepatic fibrosis and cirrhosis. On this basis, microRNAs are epigenetic regulatory elements that play important roles in liver pathology...
September 20, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/30236060/genome-wide-analysis-of-long-non-coding-rnas-in-catalpa-bungei-and-their-potential-function-in-floral-transition-using-high-throughput-sequencing
#4
Zhi Wang, Tianqing Zhu, Wenjun Ma, Nan Wang, Guanzheng Qu, Shougong Zhang, Junhui Wang
BACKGROUND: Long non-coding RNAs (lncRNAs) have crucial roles in various biological regulatory processes. However, the study of lncRNAs is limited in woody plants. Catalpa bungei is a valuable ornamental tree with a long cultivation history in China, and a deeper understanding of the floral transition mechanism in C. bungei would be interesting from both economic and scientific perspectives. RESULTS: In this study, we categorized C. bungei buds from early flowering (EF) and normal flowering (NF) varieties into three consecutive developmental stages...
September 20, 2018: BMC Genetics
https://www.readbyqxmd.com/read/30233883/serum-micrornas-improving-the-diagnostic-accuracy-in-lung-cancer-presenting-with-pulmonary-nodules
#5
Yayi He, Shengxiang Ren, Yan Wang, Xuefei Li, Caicun Zhou, Fred R Hirsch
Background: MicroRNA (miRNA) is an approach for early diagnosing of cancer. We validated a panel of miRNAs (hsa-miR-199a-3p, hsa-miR-148a-3p, hsa-miR-210-3p, hsa-miR-378d and hsa-miR-138-5p) to aid early diagnosis of lung adenocarcinoma by blood test in lung cancer presenting with pulmonary nodules. Methods: A total of 369 individuals who were detected pulmonary nodules by computed tomography (CT) scan were enrolled into this study. These patients included 274 pulmonary malignant or borderline lung diseases and 122 lung benign pulmonary nodules...
August 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/30231073/shifts-in-ovine-cardiopulmonary-microrna-expression-in-late-gestation-and-the-perinatal-period
#6
Ramona H Krauss, Belinda Phipson, Alicia Oshlack, Nikita Prasad-Gupta, Michael M Cheung, Joseph J Smolich, Salvatore Pepe
BACKGROUND: MicroRNAs (miRNAs) have been identified as important contributors to the regulation of early fetal cardiopulmonary development. However, miRNA expression profiles during late gestation and the early neonatal period are not fully elaborated in large mammals such as sheep (ovis aries). The aim of this study was to sequence miRNA from cardiopulmonary tissues in late gestation and neonate sheep to identify changes in miRNA expression. METHODS: Illumina HiSeq next-generation deep sequencing (NGS) was performed on ovine tissues from the left (LV) and right ventricles (RV), lungs and pulmonary artery (PA) of preterm fetuses (128 days), near-term fetuses (140 days) (term = 148 days) and neonatal lambs (5 days)...
2018: PloS One
https://www.readbyqxmd.com/read/30229657/gold-nanoparticle-couples-with-entropy-driven-toehold-mediated-dna-strand-displacement-reaction-on-magnetic-beads-toward-ultrasensitive-energy-transfer-based-photoelectrochemical-detection-of-mirna-141-in-real-blood-sample
#7
Nan Zhang, Xiao-Mei Shi, Hong-Qian Guo, Xiaozhi Zhao, Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Highly stable circulating microRNAs (miRNAs) are currently recognized as a novel potential biomarker for clinical cancer diagnosis in the early stage. However, limited by its low concentration, high sequence similarity as well as the numerous interferences in body fluids, detection of miRNA in whole blood with sufficient selectivity and sensitivity is still challenging. Herein, we reported the integration of entropy-driven toehold-mediated DNA strand displacement (ETSD) reaction with magnetic beads (MB) toward the energy-transfer-based photoelectrochemical (PEC) detection of the prostate carcinoma (PCa) biomarker miRNA-141 in real blood sample...
September 19, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30227605/the-dual-role-of-micrornas-in-colorectal-cancer-progression
#8
REVIEW
Lei Ding, Zhenwei Lan, Xianhui Xiong, Hongshun Ao, Yingting Feng, Huan Gu, Min Yu, Qinghua Cui
Colorectal cancer (CRC) is responsible for one of the major cancer incidence and mortality worldwide. It is well known that MicroRNAs (miRNAs) play vital roles in maintaining the cell development and other physiological processes, as well as, the aberrant expression of numerous miRNAs involved in CRC progression. MiRNAs are a class of small, endogenous, non-coding, single-stranded RNAs that bind to the 3'-untranslated region (3'-UTR) complementary sequences of their target mRNA, resulting in mRNA degradation or inhibition of its translation as a post-transcriptional regulators...
September 17, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30227118/microrna-23a-5p-promotes-atherosclerotic-plaque-progression-and-vulnerability-by-repressing-atp-binding-cassette-transporter-a1-g1-in-macrophages
#9
Shuai Yang, Zi-Ming Ye, Shengcai Chen, Xue-Ying Luo, Shao-Li Chen, Ling Mao, Yanan Li, Huijuan Jin, Cheng Yu, Fei-Xiang Xiang, Ming-Xing Xie, Jiang Chang, Yuan-Peng Xia, Bo Hu
Disruption of carotid vulnerable atherosclerotic plaque is responsible for acute ischemic stroke (AIS) and the early detection and intervention approach are greatly limited. Undertaking a microarray of microRNAs (miRNAs) in the plasma of AIS patients with carotid vulnerable plaques, miR-23a-5p was markedly elevated and was positively correlated with the plaque progression and vulnerability. Correspondingly, we found that miR-23a-5p expression was significantly increased in both plasma and macrophages from atherosclerosis mice...
September 15, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/30223386/diagnostic-and-prognostic-value-of-serum-mir-99a-expression-in-oral-squamous-cell-carcinoma
#10
Liang Chen, Jing Hu, Lina Pan, Xiaochun Yin, Qibao Wang, Hui Chen
BACKGROUND: Despite progress in the treatment of oral squamous cell carcinoma (OSCC) over past years, the prognosis for OSCC patients remains dismal. MicroRNA-99a (miR-99a) has been found to involve in the development of many cancer types, but its clinical role in OSCC is unclear. OBJECTIVE: The aim of this study was to explore the clinical implications of serum miR-99a in OSCC. METHODS: This study detected serum miR-99a levels in 121 OSCC cases and 55 healthy controls by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis...
August 29, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/30221475/oncogenic-microrna-signature-for-early-diagnosis-of-cervical-intraepithelial-neoplasia-and-cancer
#11
Stephanie S Liu, Karen Kl Chan, Daniel Kh Chu, Tina N Wei, Lesley Sk Lau, Siew F Ngu, Mandy My Chu, Ka Yu Tse, Philip Pc Ip, Enders Ko Ng, Annie Ny Cheung, Hextan Ys Ngan
Cervical cancer is one of the leading causes of cancer death in women globally, despite widespread use of cytology/Human Papillomavirus (HPV) screening. Here, we aimed to identify the potential role of miRNA as diagnostic biomarker in the detection of cervical pre-malignant lesions and cancer. In total, we recruited 582 patients with cervical diseases and 145 control individuals. The expression levels of six miRNAs (miR-20a, miR-92a, miR-141, miR-183*, miR-210 and miR-944) were found to be significantly up-regulated in cervical cancer and pre-malignant lesions as compared to normal cervical samples, indicating that they are oncogenic miRNAs...
September 17, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/30220214/serum-microrna-as-potential-biomarker-to-detect-breast-atypical-hyperplasia-and-early-stage-breast-cancer
#12
Xuefeng An, Hong Quan, Jinhui Lv, Lingyu Meng, Cheng Wang, Zuoren Yu, Jing Han
AIM: The present study aimed to identify microRNA (miRNA) that can be used for not only detecting early-stage breast cancer (BC) but also diagnosing atypical hyperplasia (AH). MATERIALS & METHODS: RT-qPCR detected the expression levels of miRNAs and receiver operating characteristic curves were constructed to evaluate sensitivity and specificity of the assay. RESULTS: miR-24 and miR-103a were expressed in an upward trend in serum of benign proliferative tumor subjects, while they were downregulated significantly in serum of AH (p < 0...
September 17, 2018: Future Oncology
https://www.readbyqxmd.com/read/30219071/exosomal-mir-1290-is-a-potential-biomarker-of-high-grade-serous-ovarian-carcinoma-and-can-discriminate-patients-from-those-with-malignancies-of-other-histological-types
#13
Masaki Kobayashi, Kenjiro Sawada, Koji Nakamura, Akihiko Yoshimura, Mayuko Miyamoto, Aasa Shimizu, Kyoso Ishida, Erika Nakatsuka, Michiko Kodama, Kae Hashimoto, Seiji Mabuchi, Tadashi Kimura
BACKGROUND: microRNAs (miRNAs) stably exist in circulating blood encapsulated in extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve as novel cancer biomarkers. New diagnostic markers to detect high grade serous ovarian cancer (HGSOC) are urgently needed. The aim of this study was to identify miRNAs specific to HGSOC and analyze whether serum miRNA can discriminate HGSOC patients from healthy controls or patients with ovarian malignancies of other histological types...
September 15, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/30197143/detection-of-microrna-in-cattle-serum-and-their-potential-use-to-diagnose-severity-of-johne-s-disease
#14
Sandeep K Gupta, Paul H Maclean, Siva Ganesh, Dairu Shu, Bryce M Buddle, D Neil Wedlock, Axel Heiser
Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease in ruminants, which is characterized by chronic progressive granulomatous enteritis. The infection leads to wasting and weight loss in the animals and eventually death, causing considerable production losses to the agricultural industry worldwide. Currently available ELISA- and PCR-based diagnostic tests have limited sensitivity and specificity during early MAP infection in cattle, suggesting that there is an urgent demand for alternative diagnostic tests...
September 6, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/30194772/mir-21-5p-mir-141-3p-and-mir-205-5p-levels-in-urine-promising-biomarkers-for-the-identification-of-prostate-and-bladder-cancer
#15
Nassim Ghorbanmehr, Sedigheh Gharbi, Eberhard Korsching, Mahmood Tavallaei, Behzad Einollahi, Seyed Javad Mowla
BACKGROUND: Early detection of cancers improves patients' survival and decreases the treatment cost. Unfortunately, the current methods for diagnosis of bladder and prostate cancers, two most common urothelial malignancies, suffer from a low sensitivity and specificity. MicroRNAs, as a group of endogenously produced non-coding RNAs, regulate gene expression and their expression is observed to be altered in many cancers and cancer progression phenomena. The remarkable stability of microRNAs in biofluids and their unique expression pattern in different pathological conditions make them an appealing, noninvasive diagnostic method in cancer diagnosis...
September 7, 2018: Prostate
https://www.readbyqxmd.com/read/30192375/genome-wide-microrna-analysis-of-hpv-positive-self-samples-yields-novel-triage-markers-for-early-detection-of-cervical-cancer
#16
Barbara C Snoek, Wina Verlaat, Iris Babion, Putri W Novianti, Mark A van de Wiel, Saskia M Wilting, Nienke E van Trommel, Maaike C G Bleeker, Leon F A G Massuger, Willem J G Melchers, Daoud Sie, Daniëlle A M Heideman, Peter J F Snijders, Chris J L M Meijer, Renske D M Steenbergen
Offering self-sampling for HPV testing improves the effectiveness of current cervical screening programs by increasing population coverage. Molecular markers directly applicable on self-samples are needed to stratify HPV-positive women at risk of cervical cancer (so-called triage) and to avoid over-referral and overtreatment. Deregulated microRNAs (miRNAs) have been implicated in the development of cervical cancer, and represent potential triage markers. However, it is unknown whether deregulated miRNA expression is reflected in self-samples...
September 7, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/30191988/the-potential-prognostic-and-therapeutic-application-of-tissue-and-circulating-micrornas-in-cervical-cancer
#17
REVIEW
Malihe Hasanzadeh, Mehraneh Movahedi, Marzieh Rejali, Faezeh Maleki, Mehrdad Moetamani-Ahmadi, Sima Seifi, Zeinab Hosseini, Majid Khazaei, Forouzan Amerizadeh, Gordon A Ferns, Majid Rezayi, Amir Avan
Cervical cancer (CC) is a common malignancy in women and a major cause of cancer-related mortality globally. Some novel biomarkers may enable the early diagnosis and monitoring of CC. MicroRNAs (miRNAs) are small noncoding RNAs that control gene translation at a posttranscriptional level. Hence the deregulation of these molecules can cause many diseases. There appears to be an association between aberrant miRNA expression and CC, but the molecular mechanisms involved in the development of CC remain unknown...
September 7, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30189225/ribonucleic-acid-biomarker-candidates-for-early-phase-group-detection-of-common-cancers
#18
REVIEW
Elmira Shajari, Hamidreza Mollasalehi
Cancer is considered as a challenging lethal agent around the world and its detection at early stages would help prevention of the high mortality. Among the widely used biomarkers in clinical diagnosis of cancer, extracellular non-coding RNAs as ribonucleic acid biomarkers serve as state-of-the-art candidates for molecular diagnosis. In that regard, microRNAs are of great priority mainly because of high variety and stability in body fluids. Accordingly, common miRNAs among most prevalent cancers could help us (pre)diagnose cancer with high accuracy in target samples...
September 4, 2018: Genomics
https://www.readbyqxmd.com/read/30185670/the-complex-landscape-of-micrornas-in-articular-cartilage-biology-pathology-and-therapeutic-targets
#19
REVIEW
Helal Endisha, Jason Rockel, Igor Jurisica, Mohit Kapoor
The disabling degenerative disease osteoarthritis (OA) is prevalent among the global population. Articular cartilage degeneration is a central feature of OA; therefore, a better understanding of the mechanisms that maintain cartilage homeostasis is vital for developing effective therapeutic interventions. MicroRNAs (miRs) modulate cell signaling pathways and various processes in articular cartilage via posttranscriptional repression of target genes. As dysregulated miRs frequently alter the homeostasis of articular cartilage, modulating select miRs presents a potential therapeutic opportunity for OA...
September 6, 2018: JCI Insight
https://www.readbyqxmd.com/read/30174846/the-role-of-plasma-mirnas-in-the-diagnosis-of-pulmonary-nodules
#20
Ke-Xing Xi, Xue-Wen Zhang, Xiang-Yang Yu, Wei-Dong Wang, Ke-Xiang Xi, Yong-Qiang Chen, Ying-Sheng Wen, Lan-Jun Zhang
Background: In this study, we aimed to assess the clinical utility of detection of plasma microRNAs (miRNAs) in the diagnosis of pulmonary nodules. Methods: Fifty-seven patients with pulmonary nodules who had undergone surgery were enrolled in our study from July 2016 to July 2017 at Sun Yat-sen University Cancer Center. We measured the expression levels of 12 miRNAs (miRNA-17, -146a, -200b, -182, -155, -221, -205, -126, -7, -21, -145, and miRNA-210) in plasma samples of 57 patients, including 15 benign pulmonary nodules patients and 42 malignant pulmonary nodules patients...
July 2018: Journal of Thoracic Disease
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