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MicroRNAs and early detection

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https://www.readbyqxmd.com/read/30008895/alteration-of-microrna-profiling-in-sphere-cultured-ovarian-carcinoma-cells
#1
Hwa Jun Cha, Sung Kwan An, Tae Jin Kim, Jae Ho Lee
Ovarian cancer is an aggressive and lethal cancer, which in part, can be attributed to complications in the effective detection of this disease during early stages of progression. Frequently, epithelial ovarian cancer is disseminated to the abdominal cavity and forms multicellular aggregates. This unique early metastatic event, and formation of the multicellular aggregate is implicated to provide a basis for understanding the underlying molecular mechanisms of metastasis in ovarian cancer. Therefore, a 3-dimensional (3D) sphere culture system was established in the present study to mimic the later stages of ovarian cancer...
August 2018: Oncology Letters
https://www.readbyqxmd.com/read/30003924/role-of-circulating-mir-182-and-mir-150-as-biomarkers-for-cirrhosis-and-hepatocellular-carcinoma-post-hcv-infection-in-egyptian-patients
#2
Noha Mohamed Hosni Shaheen, Naglaa Zayed, Nermine Magdi Riad, Hend H Tamim, Rasha Mohamed Hosny Shahin, Dalia A Labib, Suzan Mahrous ELsheikh, Reham Abdel Moneim, Ayman Yosry, Rania H Khalifa
In Egypt, liver diseases are exceptionally high, maintaining the highest prevalence of hepatitis C virus (HCV) worldwide, and increasing rates of hepatocellular carcinoma (HCC). Available diagnostic methods show poor performance in early diagnosis of HCC. Definite pathogenic factors contributing in the development of HCV are still lacking. MicroRNAs have been reported as promising biomarkers for cancers diagnosis and in virus-host interaction. This study was conducted to detect the role of miR-182 and miR-150 as biomarkers for development of cirrhosis and malignant transformation in HCV infected patients...
July 9, 2018: Virus Research
https://www.readbyqxmd.com/read/29997981/mirnas-as-biomarkers-and-for-the-early-detection-of-non-small-cell-lung-cancer-nsclc
#3
REVIEW
Yichao Han, Hecheng Li
Lung cancer is the most frequently diagnosed cancer and the most common cause of cancer death globally, of which 85% is non-small cell lung cancer (NSCLC). Early detection of NSCLC is essential to identify potential individuals for radical cure. Although low-dose computed tomography (LDCT) is recommended as standard screening with a mortality reduction of 20%, it displays a high false positive rate that poses an issue of overdiagnosis. MicroRNAs (miRNAs) are a group of small non-coding RNAs acting as important regulators in post-transcriptional gene expression and have been studied for their extensive role as novel biomarkers in NSCLC...
May 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29996899/circulating-micrornas-in-the-early-prediction-of-disease-recurrence-in-primary-breast-cancer
#4
Chara Papadaki, Michalis Stratigos, Georgios Markakis, Maria Spiliotaki, Georgios Mastrostamatis, Christoforos Nikolaou, Dimitrios Mavroudis, Sofia Agelaki
BACKGROUND: In primary breast cancer metastases frequently arise from a state of dormancy that may persist for extended periods of time. We investigated the efficacy of plasma micro-RNA (miR)-21, miR-23b, miR-190, miR-200b and miR-200c, related to dormancy and metastasis, to predict the outcome of patients with early breast cancer. METHODS: miRNAs were evaluated by RT-qPCR in plasma obtained before adjuvant chemotherapy. miRNA expression, classified as high or low according to median values, correlated with relapse and survival...
July 11, 2018: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29981251/branched-rolling-circle-amplification-method-for-measuring-serum-circulating-microrna-levels-for-early-breast-cancer-detection
#5
Tingting Fan, Yu Mao, Qinsheng Sun, Feng Liu, Jin-Shun Lin, Yajie Liu, Junwei Cui, Yuyang Jiang
Serum circulating microRNAs (c-miRNAs) are serving as useful biomarkers for cancer diagnosis. Here, we describe the development of a one-step branched rolling circle amplification (BRCA) method to measure serum c-miRNAs levels for early diagnosis of breast cancer. Four c-miRNAs, c-miRNA16 (c-miR-16), c-miRNA21 (c-miR-21), c-miRNA155 (c-miR-155), and c-miRNA195 (c-miR-195) were isolated from the serum of 49 breast cancer patients (stages I-IV) and 19 healthy controls, and analyzed using one-step BRCA. The serum levels of c-miR16, c-miR21, c-miR155, and c-miR195 were higher (P < 0...
July 7, 2018: Cancer Science
https://www.readbyqxmd.com/read/29977411/a-preliminary-study-of-microrna-208b-after-acute-myocardial-infarction-impact-on-6-month-survival
#6
Mostafa Alavi-Moghaddam, Mohammad Chehrazi, Shamila D Alipoor, Maryam Mohammadi, Alireza Baratloo, Mohammad Parsa Mahjoub, Mehrnaz Movasaghi, Johan Garssen, Ian M Adcock, Esmaeil Mortaz
Introduction: miRNAs contribute to a variety of essential biological processes including development, proliferation, differentiation, and apoptosis. Circulating microRNAs are very stable and have shown potential as biomarkers of cardiovascular disease. microRNA-208b expression was increased in the blood of patients with acute myocardial infarction (AMI) and has been proposed as a biomarker for early diagnosis. In this pilot study, we investigate the potential of circulating miR-208b as a prognostic biomarker of 6-month survival in AMI patients...
2018: Disease Markers
https://www.readbyqxmd.com/read/29973278/association-of-the-peripheral-blood-levels-of-circulating-micrornas-with-both-recurrent-miscarriage-and-the-outcomes-of-embryo-transfer-in-an-in-vitro-fertilization-process
#7
Qian Yang, Wen-Wen Gu, Yan Gu, Na-Na Yan, Yan-Yan Mao, Xing-Xing Zhen, Jian-Mei Wang, Jing Yang, Hui-Juan Shi, Xuan Zhang, Jian Wang
BACKGROUND: Implantation failure is not only a major cause of early pregnancy loss, but it is also an obstacle to assisted reproductive technologies. The identification of potential circulating biomarkers for recurrent miscarriage (RM) and/or recurrent implantation failure would contribute to the development of novel diagnosis and prediction techniques. METHODS: MiR (miR-23a-3p, 27a-3p, 29a-3p, 100-5p, 127-3p and 486-5p) expression in the villi, decidual tissues and peripheral blood plasma and serum were validated by qPCR, and the localization of miRs in the villi and decidual tissues of RM and normal pregnancy (NP) women were detected by in situ hybridization...
July 4, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29971780/evaluation-of-circulating-ebv-microrna-bart2-5p-in-facilitating-early-detection-and-screening-of-nasopharyngeal-carcinoma
#8
Chen Jiang, Jinna Chen, Shanghang Xie, Lifang Zhang, Yanqun Xiang, Maria Lung, Ngar-Woon Kam, Dora Lai-Wan Kwong, Sumei Cao, Xin-Yuan Guan
Nasopharyngeal carcinoma is an Epstein-Barr Virus (EBV) associated malignancy which is highly prevalent in Southeast Asia. EBV-related antibodies have been widely used as screening markers for early nasopharyngeal carcinoma (NPC) detection. However, due to low positive predictive rate, it is essential to develop new biomarkers to facilitate NPC early diagnosis or triage EBV serological high-risk individuals to improve the chance of NPC early detection. BART microRNAs, which are encoded by BamHI region of EBV, were reported to be abundant in NPC and have potential value in early diagnosis of NPC...
July 4, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29963250/next-generation-sequencing-identifies-mirna-based-biomarker-panel-for-lupus-nephritis
#9
Yu-Jih Su, I-Chun Lin, Lin Wang, Cheng-Hsien Lu, Yi-Ling Huang, Ho-Chang Kuo
The symptomatology of lupus nephritis (LN) consists of foamy urine and lower leg edema, as well as such systemic manifestations as oral ulcers, arthralgia/arthritis, and lymphadenopathy. However, these symptoms may appear mild and non-specific. If these symptoms are unrecognized, thus delaying treatment, approximately 10% of LN patients will develop permanent kidney damage and end-stage kidney disease. Therefore, the purpose of this study is to identify a surrogate biomarker for the early detection of LN. In this study, we first adopted next generation sequencing (NGS) in order to screen differential expression levels of microRNA between SLE patients with and without LN...
June 15, 2018: Oncotarget
https://www.readbyqxmd.com/read/29963227/identification-of-differentially-expressed-mirnas-and-mirna-targeted-genes-in-bladder-cancer
#10
Jong-Young Lee, Seok Joong Yun, Pildu Jeong, Xuan-Mei Piao, Ye-Hwan Kim, Jihye Kim, Sathiyamoorthy Subramaniyam, Young Joon Byun, Ho Won Kang, Sung Phil Seo, Jayoung Kim, Jung Min Kim, Eun Sang Yoo, Isaac Y Kim, Sung-Kwon Moon, Yung Hyun Choi, Wun-Jae Kim
Background: Differentially expressed genes and their post-transcriptional regulator-microRNAs (miRNAs), are potential keys to pioneering cancer diagnosis and treatment. The aim of this study was to investigate how the miRNA-mRNA interactions might affect the tumorigenesis of bladder cancer (BC) and to identify specific miRNA and mRNA genetic markers in the two BC types: non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). Results: We identified 227 genes that interacted with 54 miRNAs in NMIBC, and 14 genes that interacted with 10 miRNAs in MIBC...
June 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29961324/visualizing-mir-155-to-monitor-breast-tumorigenesis-and-response-to-chemotherapeutic-drugs-by-a-self-assembled-photoacoustic-nanoprobe
#11
Wenhua Cao, Wen Gao, Zhenhua Liu, Wenjing Hao, Xiang Li, Yuhui Sun, Lili Tong, Bo Tang
MicroRNA-155 (miR-155), which facilitates breast tumor growth and invasion by promoting tumor cell proliferation and inhibiting cell apoptosis, is considered an ideal early diagnostic and prognostic marker. Herein, we developed a self-assembled hybridization chain reaction (HCR)-based photoacoustic (PA) nanoprobe for highly sensitive in situ monitoring of dynamic changes in miR-155 expression during breast tumorigenesis and chemotherapy. The PA nanoprobes (Au-H1/PEG and Au-H2/PEG) were constructed by linking polyethylene glycol (PEG) and two hairpin DNA strands (H1 and H2, respectively) to the surface of gold nanoparticles (AuNPs)...
July 2, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29952206/dna-logic-operation-with-nanopore-decoding-to-recognize-microrna-patterns-in-small-cell-lung-cancer
#12
Moe Hiratani, Ryuji Kawano
Although DNA computation has traditionally been developed for parallel calculations in molecular analyses, this approach has recently been considered for use in diagnostic or medical applications in living systems. In this study, we propose that the DNA logic operation may be a powerful tool for the recognition of microRNA patterns, which may have applications for the early diagnosis of cancers. We developed a rapid, label-free decoding method for output diagnostic molecules using nanopore measurements. We designed diagnostic DNAs that autonomously recognized two microRNAs, miR-20a and miR-17-5p, and formed a four-way junction structure that was captured in the nanopore, showing long blocking currents...
June 28, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29951375/extracellular-vesicles-in-prostate-cancer-carcinogenesis-diagnosis-and-management
#13
REVIEW
Virginie Vlaeminck-Guillem
Extracellular vesicles (EVs), especially exosomes, are now well recognized as major ways by which cancer cells interact with each other and stromal cells. The meaningful messages transmitted by the EVs are carried by all components of the EVs, i.e., the membrane lipids and the cargo (DNAs, RNAs, microRNAs, long non-coding RNAs, proteins). They are clearly part of the armed arsenal by which cancer cells obtain and share more and more advantages to grow and conquer new spaces. Identification of these messages offers a significant opportunity to better understand how a cancer occurs and then develops both locally and distantly...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29942793/blood-serum-from-head-and-neck-squamous-cell-carcinoma-patients-induces-altered-microrna-and-target-gene-expression-profile-in-treated-cells
#14
Brittany Allen, Augusto Schneider, Berta Victoria, Yury O Nunez Lopez, Mark Muller, Mateusz Szewczyk, Jakub Pazdrowski, Ewa Majchrzak, Wojciech Barczak, Wojciech Golusinski, Pawel Golusinski, Michal M Masternak
The head and neck squamous cell carcinoma (HNSCC) represents one of the most common cancers in humans. Close to 600,000 new diagnoses are made every year worldwide and over half of diagnosed patients will not survive. In view of this low survival rate, the development of novel cell-based assays for HNSCC will allow more mechanistic approaches for specific diagnostics for each individual patient. The cell-based assays will provide more informative data predicting cellular processes in treated patient, which in effect would improve patient follow up...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29924598/an-ultrasensitive-diagnostic-biochip-based-on-biomimetic-periodic-nanostructure-assisted-rolling-circle-amplification
#15
Qian Yao, Yingqian Wang, Jie Wang, Shaomin Chen, Haoyang Liu, Zhuoran Jiang, Xiaoe Zhang, Song-Mei Liu, Quan Yuan, Xiang Zhou
Developing portable and sensitive devices for point of care (POC) detection of low abundance bioactive molecules is highly valuable in early diagnosis of disease. Herein, an ultrasensitive photonic crystal-assisted rolling circle amplification (PC-RCA) biochip was constructed and further applied to serum circulating microRNAs (miRNAs) detection. The biochip integrates the optical signal enhancement capability of biomimetic PC surface with the thousand-fold signal amplification feature of RCA. The biomimetic PC displayed periodic dielectric nanostructure and significantly enhanced the signal intensity of RCA reaction, leading to efficient improvement of detection sensitivity...
June 20, 2018: ACS Nano
https://www.readbyqxmd.com/read/29922886/evaluation-of-serum-paired-microrna-ratios-for-differential-diagnosis-of-non-small-cell-lung-cancer-and-benign-pulmonary-diseases
#16
Lihong Fan, Jie Sha, Junliang Teng, Dan Li, Changhui Wang, Qing Xia, Hao Chen, Bo Su, Huiwei Qi
BACKGROUND AND OBJECTIVE: To clarify whether there are different expressions between lung cancer and benign pulmonary diseases, we studied seven microRNAs (miRNAs) in serum from patients with non-small cell lung cancer (NSCLC), benign pulmonary nodules and four pulmonary inflammation diseases. METHODS: We detected the expression of miRNAs using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). RESULTS: We found that five miRNA ratios-miR-15b-5p/miR-146b-3p, miR-20a-5p/miR-146b-3p, miR-19a-3p/miR-146b-3p, miR-92a-3p/miR-146b-3p, and miR-16-5p/miR-146b-3p-show higher expression in the NSCLC group than the benign pulmonary nodule group, and 13 ratios of miRNAs were significantly upregulated in the NSCLC group compared with the pulmonary inflammation diseases group...
June 19, 2018: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/29914087/biomarkers-for-early-diagnosis-and-prognosis-of-malignant-pleural-mesothelioma-the-quest-goes-on
#17
Caterina Ledda, Paola Senia, Venerando Rapisarda
Malignant pleural mesothelioma (MM) is a highly aggressive tumor characterized by a poor prognosis. Although its carcinogenesis mechanism has not been strictly understood, about 80% of MM can be attributed to occupational and/or environmental exposure to asbestos fibers. The identification of non-invasive molecular markers for an early diagnosis of MM has been the subject of several studies aimed at diagnosing the disease at an early stage. The most studied biomarker is mesothelin, characterized by a good specificity, but it has low sensitivity, especially for non-epithelioid MM...
June 15, 2018: Cancers
https://www.readbyqxmd.com/read/29902383/review-of-recent-efforts-to-discover-biomarkers-for-early-detection-monitoring-prognosis-and-prediction-of-treatment-responses-of-patients-with-gastric-cancer
#18
Koichi Sawaki, Mitsuro Kanda, Yasuhiro Kodera
Gastric cancer (GC) is the leading cause of cancer-related death worldwide. Despite recent advances in diagnosis and therapy, the prognosis of patients with GC is poor. Many patients have inoperable disease upon diagnosis or experience recurrent disease after curative gastrectomy. Unfortunately, tumor markers for GC, such as serum carcinoembryonic antigen and carbohydrate antigen 19-9, lack sufficient sensitivity and specificity. Therefore, effective biomarkers are required to detect early GC and to predict tumor recurrence and chemosensitivity...
June 14, 2018: Expert Review of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29901577/serum-mir-17-il-4-and-il-6-levels-for-diagnosis-of-endometriosis
#19
Fang Wang, Hongxia Wang, Danting Jin, Yang Zhang
Clinical studies have exhibited microRNAs or cytokines could be used as new biomarkers in the diagnosis of endometriosis, respectively. The purpose of this study was to investigate the role of serum miR-17, IL-4, and IL-6 as early diagnostic markers of endometriosis. One hundred forty patients aged 22 to 45 years were recruited, 80 patients with pathologically confirmed endometriosis were assigned to endometriosis group whereas the remaining 60 patients were in the control group. The blood samples were collected immediately before laparoscopy and analyzed using real-time quantitative PCR analysis...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29901178/mir%C3%A2-150-promotes-the-proliferation-and-migration-of-non%C3%A2-small-cell-lung-cancer-cells-by-regulating-the-sirt2-jmjd2a-signaling-pathway
#20
Kanqiu Jiang, Mingjing Shen, Yongbing Chen, Weihua Xu
Lung cancer (LC), as the most common cause of cancer‑related mortality worldwide, is characterized by difficulties in early detection, a high degree of malignancy, poor sensitivity to radiotherapy and chemotherapy, and a low 5‑year survival rate. MicroRNA (miRNA) is a class of small, non‑coding, endogenously expressed RNA that serves vital roles in RNA silencing and post‑transcriptional regulation of gene expression. Previous studies have shown that abnormal expression of miRNA is relevant to various malignant tumors, including lung cancer...
June 12, 2018: Oncology Reports
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