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MicroRNAs and early detection

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https://www.readbyqxmd.com/read/29467585/integrating-circulating-immunological-and-sputum-biomarkers-for-the-early-detection-of-lung-cancer
#1
Jian Su, Qixin Leng, Yanli Lin, Jie Ma, Fangran Jiang, Cheng-Ju Lee, HongBin Fang, Feng Jiang
We have demonstrated that assessments of microRNA (miRNA) expressions in circulating peripheral blood mononucleated cell (PBMC) and sputum specimens, respectively, may help diagnose lung cancer. To assess the individual and combined analysis of the miRNAs across the different body fluids for lung cancer early detection, we analyse a panel of 3 sputum miRNAs (miRs-21, 31, and 210) and a panel of 2 PBMC miRNAs (miRs-19b-3p and 29b-3p) in a discovery cohort of 68 patients with lung cancer and 66 cancer-free smokers...
2018: Biomarkers in Cancer
https://www.readbyqxmd.com/read/29445851/beta-cell-extracellular-vesicle-mir-21-5p-cargo-is-increased-in-response-to-inflammatory-cytokines-and-serves-as-a-biomarker-of-type-1-diabetes
#2
Alexander J Lakhter, Rachel E Pratt, Rachel E Moore, Kaitlin K Doucette, Bernhard F Maier, Linda A DiMeglio, Emily K Sims
AIMS/HYPOTHESIS: Improved biomarkers are acutely needed for the detection of developing type 1 diabetes, prior to critical loss of beta cell mass. We previously demonstrated that elevated beta cell microRNA 21-5p (miR-21-5p) in rodent and human models of type 1 diabetes increased beta cell apoptosis. We hypothesised that the inflammatory milieu of developing diabetes may also increase miR-21-5p in beta cell extracellular vesicle (EV) cargo and that circulating EV miR-21-5p would be increased during type 1 diabetes development...
February 14, 2018: Diabetologia
https://www.readbyqxmd.com/read/29440644/ultrasensitive-optical-biosensor-for-detection-of-mirna-155-using-positively-charged-au-nanoparticles
#3
Fatemeh Hakimian, Hedayatollah Ghourchian, Azam Sadat Hashemi, Mohammad Reza Arastoo, Mohammad Behnam Rad
An ultrasensitive optical biosensor for microRNA-155 (miR-155) was developed to diagnose breast cancer at early stages. At first, the probe DNA covalently bind to the negatively charged gold nanoparticles (citrate-capped AuNPs). Then, the target miR-155 electrostatically adsorb onto the positively charged gold nanoparticles (polyethylenimine-capped AuNP) surface. Finally, by mixing citrate-capped AuNP/probe and polyethylenimine-capped AuNP/miR-155, hybridization occurs and the optical signal of the mixture give a measure to quantify the miR-155 content...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29440068/molecular-characterization-of-renal-cell-carcinoma-a-potential-three-microrna-prognostic-signature
#4
Soum D Lokeshwar, Asif Talukder, Travis J Yates, Martin J P Hennig, Michael Garcia-Roig, Sarrah S Lahorewala, Naureen N Mullani, Zachary Klaassen, Bruce R Kava, Murugesan Manoharan, Mark S Soloway, Vinata Lokeshwar
BACKGROUND: Aberrantly expressed microRNAs (miR) promote renal cell carcinoma (RCC) growth and metastasis and are potentially useful biomarkers for metastatic disease. However, a consensus clinically significant miRNA signature has not been identified. To identify a miRNA signature for predicting clinical outcome in RCC patients we used a four-pronged interconnected approach. METHODS: Differentially expressed miRs were identified and analyzed in 113 specimens (Normal kidney: 59; tumor: 54)...
February 12, 2018: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/29435003/opposite-response-to-hypoxia-by-breast-cancer-cells-between-cell-proliferation-and-cell-migration-a-clue-from-microrna-expression-profile
#5
Ming Zhang, Chang-E Gao, Wen-Lin Chen, Yi-Yin Tang, Jian-Yun Nie, Li-Da Shen, Xiang Ma, De-Dian Chen
The majority of tumors possess the features of hypoxia. It is generally accepted that hypoxia is a negative prognostic factor for cancer. Low levels of oxygen are able to modify basic cell metabolism status. Elucidating the basic response, including cell proliferation and migration, to hypoxia by cancer cells is important for understanding the role of hypoxia in the development of cancer. In the present study, CoCl2 stimulation was used to simulate hypoxia. A microRNA (miRNA/miR) array was used to systematically detect the changes in miRNA expression profiles...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434754/microrna-191-5p-exerts-a-tumor-suppressive-role-in-renal-cell-carcinoma
#6
Peijie Chen, Xiang Pan, Liwen Zhao, Lu Jin, Canbin Lin, Jing Quan, Tao He, Liang Zhou, Xueling Wu, Yong Wang, Liangchao Ni, Shangqi Yang, Yongqing Lai
Renal cell carcinoma (RCC) is a common tumor of the urinary system. Previously, miR-191-5p has been reported to be associated with various types of cancer; however, its specific functions in RCC have not been investigated to date. In the present study, the expression of miR-191-5p in the 786-O and ACHN cell lines was detected in vitro by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results of RT-qPCR revealed that miR-191-5p was significantly downregulated in the two cell lines compared with the 293T cell line...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29434752/microrna-152-inhibits-ovarian-cancer-cell-proliferation-and-migration-and-may-infer-improved-outcomes-in-ovarian-cancer-through-targeting-foxp1
#7
Wen Qin, Wei Xie, Qinglin He, Tianwei Sun, Chaoguo Meng, Kunling Yang, Yuanfu Luo, Dongmei Yang
microRNA (miR) are a class of endogenous small non-coding RNA that are aberrantly expressed and are critical in tumorigenesis. Amongst them, miR-152 was reported to be dysregulated in epithelial ovarian cancer (EOC). However, the function and mechanism of miR-152 is not well understood. In the present study, total RNA was extracted from 58 ovarian epithelial carcinoma tissue samples and adjacent non-tumor tissues and measured by reverse transcription-quantitative polymerase chain reaction. The observations of the present study revealed that the expression of miR-152 was significantly downregulated in EOC specimens, as well as three ovarian cancer (OC) cell lines...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29431420/a-mos2-nanoprobe-for-micrornas-quantification-based-on-duplex-specific-nuclease-signal-amplification
#8
Mingshu Xiao, Tiantian Man, Changfeng Zhu, Hao Pei, Jiye Shi, Li Li, Xiangmeng Qu, Xizhong Shen, Jiang Li
MicroRNAs (miRNAs) play significant regulatory roles in physiologic and pathologic processes and are considered as important biomarkers for disease diagnostics and therapeutics. Simple, fast, sensitive, and selective detection of miRNAs, however, is challenged by their short length, low abundance, susceptibility to degradation, and homogenous sequence. Here we develop MoS2-loaded molecular beacons (MBs) nanoprobes for highly sensitive and selective detection of miRNAs based on duplex-specific nuclease-mediated signal amplification (DSNMSA)...
February 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29430188/lincrna-ror-promotes-metastasis-and-invasion-of-esophageal-squamous-cell-carcinoma-by-regulating-mir-145-fscn1
#9
Muhe Shang, Xianghu Wang, Ying Zhang, Zhikui Gao, Tian Wang, Ran Liu
Background and objective: In an attempt to discover a new biomarker for early diagnosis and prognosis of esophageal squamous cell carcinoma (ESCC), the regulation mechanism of large intergenic non-coding RNA-regulator of reprogramming (lincRNA-ROR) as a microRNA (miRNA) sponge was studied. Patients and methods: ROR expression in 91 pairs of ESCC tissue samples and matched adjacent tissues was quantified with real-time fluorescent quantitative polymerase chain reaction (qRT-PCR)...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29426337/non-coding-rnas-and-retroviruses
#10
REVIEW
Xu Zhang, Xiancai Ma, Shuliang Jing, Hui Zhang, Yijun Zhang
Retroviruses can cause severe diseases such as cancer and acquired immunodeficiency syndrome. A unique feature in the life cycle of retroviruses is that their RNA genome is reverse transcribed into double-stranded DNA, which then integrates into the host genome to exploit the host machinery for their benefits. The metazoan genome encodes numerous non-coding RNAs (ncRNA), which act as key regulators in essential cellular processes such as antiviral response. The development of next-generation sequencing technology has greatly accelerated the detection of ncRNAs from viruses and their hosts...
February 9, 2018: Retrovirology
https://www.readbyqxmd.com/read/29422531/circulating-mirnas-as-diagnostic-biomarkers-for-adolescent-idiopathic-scoliosis
#11
José Luis García-Giménez, Pedro Antonio Rubio-Belmar, Lorena Peiró-Chova, David Hervás, Daymé González-Rodríguez, José Santiago Ibañez-Cabellos, Paloma Bas-Hermida, Salvador Mena-Mollá, Eva María García-López, Federico V Pallardó, Teresa Bas
The aetiology of adolescent idiopathic scoliosis (AIS) has been linked to many factors, such as asymmetric growth, neuromuscular condition, bone strength and genetic background. Recently, epigenetic factors have been proposed as contributors of AIS physiopathology, but information about the molecular mechanisms and pathways involved is scarce. Regarding epigenetic factors, microRNAs (miRNAs) are molecules that contribute to gene expression modulation by regulating important cellular pathways. We herein used Next-Generation Sequencing to discover a series of circulating miRNAs detected in the blood samples of AIS patients, which yielded a unique miRNA biomarker signature that diagnoses AIS with high sensitivity and specificity...
February 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29417946/micrornas-as-biomarkers-for-human-glioblastoma-progress-and-potential
#12
REVIEW
Shi-Wei Huang, Ni-da Ali, Lily Zhong, Jian Shi
Glioblastoma multiforme (GBM) is the most common malignant glioma. Despite innovative research efforts in tumor therapy, the outcome for most diagnosed patients remains poor; therefore, early diagnosis of GBM is the most effective method for achieving better patient outcomes. In recent years, combined research efforts including cellular, molecular, genetic, and bioinformatics methods have been used to investigate GBM, and the results show that variations in miRNA expression occur in GBM tissues and biological fluids...
February 8, 2018: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29417820/bidirectional-electrochemiluminescence-color-switch-an-application-in-detecting-multi-markers-of-prostate-cancer
#13
Yinzhu Wang, Si-Yuan Ji, Heng-Yu Xu, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
A selective excitation of [Ir(df-ppy)2(pic)] and [Ru(bpy)3]2+ through tuning electrode potential is reported in this work. Bidirectional color change from blue-green to red could be observed along with increase and decrease of the potential, which was ascribed to the dual-potential excitation property of [Ir(df-ppy)2(pic)]. Similar to three-electrode system, selective excitation of ECL could be achieved at the anode of bipolar electrode (BPE). Both increase and decrease of the faradic reactions at the cathode of the BPE could induce ECL reporting color at the other pole switched from blue-green to red...
February 8, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29412128/variation-in-immune-related-micrornas-profile-in-human-milk-amongst-lactating-women
#14
Mariarita Perri, Maria Lucente, Roberto Cannataro, Ida Francesca De Luca, Luca Gallelli, Concetta Cafiero, Giovambattista De Sarro, Guido Moro, Maria Cristina Caroleo, Erika Cione
BACKGROUND: Human milk (HM) is a biological fluid representing the first nutriment for newborns. It directly impacts the development of the infant's immune system. In this concern, specific microRNAs (miRNAs) such as hsa-miR-21, hsa-miR-181a, hsa-miR-150 and hsa-miR-223 are known to be involved in the innate and acquired immune response. OBJECTIVE: Herein, these miRNAs were evaluated in frozen and pasteurized samples of human colostrum and HM in order to elucidate the distribution and the expression profile of these biological mediators in both biological fluids...
February 6, 2018: MicroRNA
https://www.readbyqxmd.com/read/29409452/whole-blood-micrornas-as-potential-biomarkers-in-post-operative-early-breast-cancer-patients
#15
Marianna Alunni-Fabbroni, Leonie Majunke, Elisabeth K Trapp, Marie Tzschaschel, Sven Mahner, Peter A Fasching, Tanja Fehm, Andreas Schneeweiss, Thomas Beck, Ralf Lorenz, Thomas W P Friedl, Wolfgang Janni, Brigitte Rack
BACKGROUND: microRNAs (miRNAs) are considered promising cancer biomarkers, showing high reliability, sensitivity and stability. Our study aimed to identify associations between whole blood miRNA profiles, presence of circulating tumor cells (CTCs) and clinical outcome in post-operative early breast cancer patients (EBC) to assess the utility of miRNAs as prognostic markers in this setting. METHOD: A total of 48 post-operative patients, recruited in frame of the SUCCESS A trial, were included in this retrospective study and tested with a panel of 8 miRNAs (miR-10b, -19a, - 21, - 22, -20a, - 127, - 155, -200b)...
February 6, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29398458/the-potential-role-of-micrornas-as-biomarkers-in-benign-prostatic-hyperplasia-a-systematic-review-and-meta-analysis
#16
REVIEW
Francesco Greco, Antonino Inferrera, Roberto La Rocca, Michele Navarra, Marco Casciaro, Gaetano Grosso, Sebastiano Gangemi, Vincenzo Ficarra, Vincenzo Mirone
CONTEXT: Benign prostate hyperplasia (BPH) is one of the most common urologic diseases. However, the molecular and cellular mechanisms involving the stromal and epithelial components of the prostate that lead to BPH remain unclear. OBJECTIVE: To review and evaluate the evidence implicating microRNAs (miRNAs) in the pathogenesis of BPH. EVIDENCE ACQUISITION: A systematic search of the PubMed and Embase databases was performed using the terms "benign prostate hypertrophy and miRNA" or ("benign prostate hypertrophy and microRNAs" or "miRNA" or "miR") on July 31, 2017...
February 2, 2018: European Urology Focus
https://www.readbyqxmd.com/read/29393972/evaluation-of-microrna-stability-in-feces-from-healthy-dogs
#17
Susanna Cirera, Line M Willumsen, Thea T Johansen, Lise N Nielsen
BACKGROUND: Gastrointestinal cancer accounts for approximately 8% of all canine malignancies. Early detection of cancer may have a tremendous impact on both treatment options and prognosis. MicroRNAs (miRNAs), a class of noncoding RNAs that can be found stably expressed in body fluids and feces, have been suggested as valuable human cancer biomarkers. OBJECTIVES: The purpose of the study was to investigate the feasibility of detecting miRNAs in canine feces and to determine the miRNA stability in fecal samples stored at different temperatures for different duration...
February 2, 2018: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/29393376/microrna-expression-profiling-in-placenta-and-maternal-plasma-in-early-pregnancy-loss
#18
Mohammad Kazem Hosseini, Tuba Gunel, Ece Gumusoglu, Ali Benian, Kilic Aydinli
Early pregnancy loss (EPL), also termed early miscarriage, is determined as the unintentional expulsion of an embryo or fetus prior to the 12th week of gestation. EPL frequency is ~15% in pregnancies. Fetal development and growth is associate with placental function and vessel development; therefore, the placental genome would represent a useful miscarriage model for (epi)genetic and genomic studies. An important factor of placental development and function is epigenetic regulation of gene expression. microRNAs (miRNAs) are the primary epigenetic regulators which have an important role in placental development and function...
January 31, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29385987/an-elevated-expression-of-serum-exosomal-microrna-191-%C3%A2-21-451a-of-pancreatic-neoplasm-is-considered-to-be-efficient-diagnostic-marker
#19
Takuma Goto, Mikihiro Fujiya, Hiroaki Konishi, Junpei Sasajima, Shugo Fujibayashi, Akihiro Hayashi, Tatsuya Utsumi, Hiroki Sato, Takuya Iwama, Masami Ijiri, Aki Sakatani, Kazuyuki Tanaka, Yoshiki Nomura, Nobuhiro Ueno, Shin Kashima, Kentaro Moriichi, Yusuke Mizukami, Yutaka Kohgo, Toshikatsu Okumura
BACKGROUND: Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers. METHODS: The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™...
January 31, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29385732/biomarkers-for-detecting-mitochondrial-disorders
#20
REVIEW
Josef Finsterer, Sinda Zarrouk-Mahjoub
(1) Objectives: Mitochondrial disorders (MIDs) are a genetically and phenotypically heterogeneous group of slowly or rapidly progressive disorders with onset from birth to senescence. Because of their variegated clinical presentation, MIDs are difficult to diagnose and are frequently missed in their early and late stages. This is why there is a need to provide biomarkers, which can be easily obtained in the case of suspecting a MID to initiate the further diagnostic work-up. (2) Methods: Literature review. (3) Results: Biomarkers for diagnostic purposes are used to confirm a suspected diagnosis and to facilitate and speed up the diagnostic work-up...
January 30, 2018: Journal of Clinical Medicine
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