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https://www.readbyqxmd.com/read/28934297/molecular-and-biological-characterization-of-%C3%AF-rs551-a-filamentous-bacteriophage-isolated-from-a-race-3-biovar-2-strain-of-ralstonia-solanacearum
#1
Abdelmonim Ali Ahmad, Michael J Stulberg, John Patrick Mershon, Dimitre S Mollov, Qi Huang
A filamentous bacteriophage, designated ϕRs551, was isolated and purified from the quarantine and select agent phytopathogen Ralstonia solanacearum race 3 biovar 2 strain UW551 (phylotype IIB sequevar 1) grown under normal culture conditions. Electron microscopy suggested that ϕRs551 is a member of the family Inoviridae, and is about 1200 nm long and 7 nm wide. ϕRs551 has a genome of 7929 nucleotides containing 14 open reading frames, and is the first isolated virion that contains a resolvase (ORF13) and putative type-2 phage repressor (ORF14)...
2017: PloS One
https://www.readbyqxmd.com/read/28931414/coagulase-gene-polymorphism-enterotoxigenecity-biofilm-production-and-antibiotic-resistance-in-staphylococcus-aureus-isolated-from-bovine-raw-milk-in-north-west-india
#2
Vishnu Sharma, Sanjita Sharma, Dinesh Kumar Dahiya, Aarif Khan, Manisha Mathur, Aayushi Sharma
BACKGROUND: Staphylococcus aureus is the predominant bacterium responsible for various diseases in animals and humans. Preventive strategies could be better implemented by understanding the prevalence, genetic patterns, and the presence of enterotoxin and biofilm-producing genes along with the antibiotic susceptibility of this organism. This study was conducted in Rajasthan, the northwestern state of India, holding the largest population of cattle that makes it the second largest milk producer in India and no such prior information is available on these aspects...
September 20, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28930138/antifungal-and-anticancer-potential-of-argemone-mexicana-l
#3
Nilesh V More, Arun S Kharat
Background: Medicinal plants are widely used to treat infectious diseases, metabolic disorders and cancer. Argemone mexicana L. (A. mexicana), commonly found on desolate land of Marathwada (Maharashtra, India) has been used to treat oral cavity infections. Methods: In this study, cold aqueous and methanolic extracts were prepared from A. mexicana stem and leaves. These extracts were tested for their antifungal and anticancer activities. The antifungal activity was tested using the agar well diffusion method, while the anticancer activity against immortalized cell lines was assessed by trypan blue assay...
November 3, 2016: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930011/poliovirus-excretion-in-children-with-primary-immunodeficiency-disorders-india
#4
Madhu Chhanda Mohanty, Manisha Rajan Madkaikar, Mukesh Desai, Prasad Taur, Uma Prajwal Nalavade, Deepa Kailash Sharma, Maya Gupta, Aparna Dalvi, Snehal Shabrish, Manasi Kulkarni, Jahnavi Aluri, Jagadish Mohanrao Deshpande
Prolonged excretion of poliovirus can occur in immunodeficient patients who receive oral polio vaccine, which may lead to propagation of highly divergent vaccine-derived polioviruses (VDPVs), posing a concern for global polio eradication. This study aimed to estimate the proportion of primary immunodeficient children with enterovirus infection and to identify the long-term polio/nonpolio enterovirus excreters in a tertiary care unit in Mumbai, India. During September 2014-April 2017, 151 patients received diagnoses of primary immunodeficiency (PID)...
October 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28927676/a-pilot-study-to-expand-treatment-of-chronic-hepatitis-c-in-resource-limited-settings
#5
REVIEW
Poonam Mathur, Emily Comstock, Edward McSweegan, Natalia Mercer, Nongthombam Suraj Kumar, Shyamasundaran Kottilil
The past five years have seen a revolution in the treatment of chronic hepatitis C, as short duration oral regimens of direct-acting antiviral drugs (DAAs), with nearly 100% cure rates for all genotypes, have replaced longer courses of ribavirin and injected interferon. Although initially very expensive, these DAAs are now becoming available in generic equivalents in countries with large numbers of chronically infected people, such as India. However, a number of obstacles may hinder the delivery of these drugs in resource-limited settings, including lack of access to diagnostic testing and the restriction of treatment to a small number of medical specialists...
September 16, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28927555/comparative-study-of-clinical-presentation-and-hematological-indices-in-hospitalized-sickle-cell-patients-infected-with-severe-plasmodium-falciparum-malaria
#6
Prasanta Purohit, Pradeep Kumar Mohanty, Siris Patel, Padmalaya Das, Jogeswar Panigrahi, Kishalaya Das
BACKGROUND: Sickle-cell-gene has a high frequency in malaria endemic regions. In India, though the prevalence of both sickle-cell-gene and malaria are high, no study has been carried out. This study aims to find out the possible differences in hematological and clinical parameters in severe falciparum malaria with respect to sickle cell genotypes. METHODS: Five hundred fourteen adults with severe falciparum malaria hospitalized at Department of Medicine, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, between August, 2010 to December, 2014 were included and categorized on the basis of sickle cell genotypes...
September 15, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28924461/do-the-benefits-of-transplant-tourism-amongst-nigerian-patients-outweigh-the-risks-a-single-center-experience
#7
C O Amira, B T Bello
BACKGROUND: Transplant tourism (TT) is the term used to describe travel outside one's country of abode for the sole purpose of obtaining organ transplantation services. OBJECTIVE: This study describes the characteristics and outcomes of kidney transplant tourists who were followed up in our institution. METHODS: A retrospective study was conducted on patients who underwent kidney transplantation outside the country and were followed up in our institution from 2007 to 2015...
2017: International Journal of Organ Transplantation Medicine
https://www.readbyqxmd.com/read/28923118/hypothermia-for-encephalopathy-in-low-and-middle-income-countries-helix-study-protocol-for-a-randomised-controlled-trial
#8
Sudhin Thayyil, Vania Oliveira, Peter J Lally, Ravi Swamy, Paul Bassett, Mani Chandrasekaran, Jayashree Mondkar, Sundaram Mangalabharathi, Naveen Benkappa, Arasar Seeralar, Mohammod Shahidullah, Paolo Montaldo, Jethro Herberg, Swati Manerkar, Kumutha Kumaraswami, Chinnathambi Kamalaratnam, Vinayagam Prakash, Rema Chandramohan, Prathik Bandya, Mohammod Abdul Mannan, Ranmali Rodrigo, Mohandas Nair, Siddarth Ramji, Seetha Shankaran
BACKGROUND: Therapeutic hypothermia reduces death and disability after moderate or severe neonatal encephalopathy in high-income countries and is used as standard therapy in these settings. However, the safety and efficacy of cooling therapy in low- and middle-income countries (LMICs), where 99% of the disease burden occurs, remains unclear. We will examine whether whole body cooling reduces death or neurodisability at 18-22 months after neonatal encephalopathy, in LMICs. METHODS: We will randomly allocate 408 term or near-term babies (aged ≤ 6 h) with moderate or severe neonatal encephalopathy admitted to public sector neonatal units in LMIC countries (India, Bangladesh or Sri Lanka), to either usual care alone or whole-body cooling with usual care...
September 18, 2017: Trials
https://www.readbyqxmd.com/read/28923039/a-pilot-single-centre-double-blind-placebo-controlled-randomized-parallel-study-of-calmagen%C3%A2-dermaceutical-cream-and-lotion-for-the-topical-treatment-of-tinea-and-onychomycosis
#9
Manoj Parekh, Girisha Ramaiah, Prachi Pashilkar, Ranjani Ramanujam, Peter Johnston, Leodevico L Ilag
BACKGROUND: Most of the current anti-fungal treatments are chemical-based, fungistatic, have low efficacy in the treatment of tinea and toxicity concerns, while onychomycosis remains recalcitrant to most antifungal therapies. The study aimed to establish the fungicidal, efficacy and safety profile of Calmagen® dermaceutical cream and lotion containing AMYCOT® as a topical treatment in patients with severe to very severe presentations of fungal skin (tinea) and nail infections (onychomycosis)...
September 18, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28923009/apophysomyces-variabilis-draft-genome-sequence-and-comparison-of-predictive-virulence-determinants-with-other-medically-important-mucorales
#10
Hariprasath Prakash, Shivaprakash Mandya Rudramurthy, Prasad S Gandham, Anup Kumar Ghosh, Milner M Kumar, Chandan Badapanda, Arunaloke Chakrabarti
BACKGROUND: Apophysomyces species are prevalent in tropical countries and A. variabilis is the second most frequent agent causing mucormycosis in India. Among Apophysomyces species, A. elegans, A. trapeziformis and A. variabilis are commonly incriminated in human infections. The genome sequences of A. elegans and A. trapeziformis are available in public database, but not A. variabilis. We, therefore, performed the whole genome sequence of A. variabilis to explore its genomic structure and possible genes determining the virulence of the organism...
September 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28921677/clinical-profile-of-hepatitis-c-virus-infection-in-a-developing-country-india
#11
Ramit Mahajan, Vandana Midha, Omesh Goyal, Varun Mehta, Vikram Narang, Kirandeep Kaur, Arshdeep Singh, Dharmatma Singh, Rishu Bhanot, Ajit Sood
BACKGROUND: The epidemiology and clinical profile of hepatitis C virus (HCV) varies worldwide and data from developing countries is sparse. AIMS: To study the clinical profile of HCV infection in a developing country in South East Asia (India). METHODS: This observational study assessed patient demographics, viral characteristics, risk factors for virus acquisition and disease characteristics in HCV patients diagnosed between January 2004 and December 2015...
September 18, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28915849/exposure-rate-of-vzv-among-women-attending-antenatal-care-clinic-in-sri-lanka-a-cross-sectional-study
#12
Saluwadana Walawwe Pavithra Lakmini Daulagala, Faseeha Noordeen, Mohamed Mukthar Fathina Fara, Chathura Rathnayake, Kapila Gunawardana
BACKGROUND: Varicella or chickenpox was not a notifiable disease until 2005 in Sri Lanka and only a few studies have been conducted on the epidemiology of VZV infection in the country. The anti-VZV IgG sero-prevalence among antenatal women is extremely limited and thus a selected group of antenatal clinic attendees were chosen to determine the exposure rate to VZV infection. METHODS: Women attending the antenatal clinic at Teaching Hospital, Peradeniya, Sri Lanka were selected for the study and 3 mL of venous blood was collected from 181 participants and the demographic data was obtained through a pre-tested questionnaire...
September 16, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28910810/concurrent-infection-with-plasmodium-vivax-and-the-dengue-and-chikungunya-viruses-in-a-paediatric-patient-from-new-delhi-india-in-2016
#13
Ayesha Tazeen, Mohd Abdullah, Malik Hisamuddin, Sher Ali, Irshad H Naqvi, Hirday N Verma, Anwar Ahmed, Shama Parveen
Dengue and chikungunya fevers are transmitted by the common mosquito vector Aedes and malaria by Anopheles. Concurrent infections are reported due to co-circulation of these pathogens, especially in endemic regions. We report a rare case of triple infection with 3 arthropod-borne pathogens (Plasmodium vivax and the dengue and chikungunya viruses) in a 3-year-old child from New Delhi, India, in August 2016. The viruses were identified by RT-PCR and the parasite by microscopy and antigen detection. The dengue virus serotype 3 sequence was clustered in the genotype III by the phylogenetic analysis...
September 15, 2017: Intervirology
https://www.readbyqxmd.com/read/28904924/rare-opportunistic-bread-mold-fungal-infection-of-maxillary-sinus-in-a-diabetic-patient
#14
Nandini Bhaskar, Vipin Chhabra, Nitin Kaushal, Salil Aggarwal
Mucormycosis is a rare potentially fatal opportunistic fungal infection that affects human beings. Normally, a healthy is immune to such infections but there are some risk factors which predispose a person to mucormycosis, of which malnutrition and diabetes mellitus are the most common risk factors. India is the most commonly affected country by mucormycosis because of high proportion people with low socioeconomic status and diabetes mellitus. In diabetes mellitus, mucormycosis is more aggressive and fatal due to an impaired host defense mechanism...
July 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28904915/microbiological-surveillance-of-operation-theatres-five-year-retrospective-analysis-from-a-tertiary-care-hospital-in-north-india
#15
Dipender Kaur Najotra, Aneeta Singh Malhotra, Poonam Slathia, Shivani Raina, Ashok Dhar
INTRODUCTION: Microbiological contamination of air and environment in the operation theaters (OTs) are major risk factor for surgical site and other hospital-associated infections. OBJECTIVES: The aim was to identify bacterial colonization of surfaces and equipment and to determine the microbial contamination of air in the OTs of a tertiary care hospital. MATERIALS AND METHODS: Five years (January 2010-December 2014) retrospective analysis of the data obtained from routine microbiological surveillance of the five OTs of the hospital was done...
July 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28904445/epidemiology-of-peripheral-neuropathy-an-indian-perspective
#16
REVIEW
Sweety Trivedi, Alak Pandit, Goutam Ganguly, Shyamal Kumar Das
Peripheral neuropathy (PN) is a common disorder and presents as diagnostic and therapeutic challenge to physicians and neurologists. In epidemiological studies from India from various regions the overall prevalence of PN varied from 5 to 2400 per 10,000 population in various community studies. India is composed of a multiethnic, multicultural population who are exposed to different adverse environmental factors such as arsenic and lead. Use of different chemotherapeutic agents with propensity to affect peripheral nerves, increasing methods of diagnosis of connective tissue disorders and use of immunomodulating drugs, growing aging population is expected to change the spectrum and burden of peripheral neuropathy in the community...
July 2017: Annals of Indian Academy of Neurology
https://www.readbyqxmd.com/read/28904433/risk-factors-for-urinary-tract-infections-in-renal-allograft-recipients-experience-of-a-tertiary-care-center-in-hyderabad-south-india
#17
M V N L R Mohan, M Neeraja, S Sudhaharan, S B Raju, T Gangadhar, V Lakshmi
Renal transplantation is an effective and commonly performed procedure for end-stage renal disease. Urinary tract infections are a major cause of morbidity and mortality in renal transplant patients. As data on postrenal transplant urinary tract infections from the Indian subcontinent are limited, the present study was conducted to estimate the burden of urinary tract infections in this vulnerable group of patients. This was a prospective study on patients undergoing renal transplantation in 2014 at our tertiary hospital in South India with a follow-up of 2 years to evaluate the risk factors for urinary tract infections...
September 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/28902135/point-prevalence-surveys-of-antimicrobial-use-among-hospitalized-children-in-six-hospitals-in-india-in-2016
#18
Sumanth Gandra, Sanjeev K Singh, Dasaratha R Jinka, Ravishankar Kanithi, Ashok K Chikkappa, Anita Sharma, Dhanya Dharmapalan, Anil Kumar Vasudevan, Onkaraiah Tunga, Akhila Akula, Garima Garg, Yingfen Hsia, Srinivas Murki, Gerardo Alvarez-Uria, Mike Sharland, Ramanan Laxminarayan
The prevalence of antimicrobial resistance in India is among the highest in the world. Antimicrobial use in inpatient settings is an important driver of resistance, but is poorly characterized, particularly in hospitalized children. In this study, conducted as part of the Global Antimicrobial Resistance, Prescribing, and Efficacy in Neonates and Children (GARPEC) project, we examined the prevalence of and indications of antimicrobial use, as well as antimicrobial agents used among hospitalized children by conducting four point prevalence surveys in six hospitals between February 2016 and February 2017...
September 13, 2017: Antibiotics
https://www.readbyqxmd.com/read/28901019/isolation-and-characterization-of-pathogenic-vibrio-alginolyticus-from-sea-cage-cultured-cobia-rachycentron-canadum-linnaeus-1766-%C3%A2-in-india
#19
P Rameshkumar, A K A Nazar, M A Pradeep, C Kalidas, R Jayakumar, G Tamilmani, M Sakthivel, A K Samal, S Sirajudeen, V Venkatesan, B M Nazeera
Mass mortalities of cobia Rachycentron canadum sub-adults occurred during August 2013 in cage culture at the Gulf of Mannar, Mandapam Tamil Nadu, India. The epizootic of disease was started with typical classical clinical signs followed by the acute mortality. Grossly, severe haemorrhage and congestion was observed in the gastric mucosa. Abdomen was distended with peritoneal fluid. Heart revealed haemopericardium and fibrinous pericardium. Histologically, the gastric mucosa showed severe erosion and necrosis...
September 12, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28900620/can-mixed-parasite-infections-thwart-targeted-malaria-elimination-program-in-india
#20
Upasana Shyamsunder Singh, Nisha Siwal, Veena Pande, Aparup Das
India is highly endemic to malaria with prevalence of all five species of human malaria parasites of Plasmodium genus. India is set for malaria elimination by 2030. Since cases of mixed Plasmodium species infections remain usually undetected but cause huge disease burden, in order to understand the distributional prevalence of both monospecies infections and mixed species infections in India, we collated published data on the differential infection incidences of the five different malaria parasites based on PCR diagnostic assay...
2017: BioMed Research International
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