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https://www.readbyqxmd.com/read/29053708/ssn6-tup1-global-transcriptional-co-repressor-role-of-the-n-terminal-glutamine-rich-region-of-ssn6
#1
Athanassios Tartas, Christoforos Zarkadas, Maria Palaiomylitou, Niki Gounalaki, Dimitris Tzamarias, Metaxia Vlassi
The Ssn6-Tup1 complex is a general transcriptional co-repressor formed by the interaction of Ssn6, a tetratricopeptide repeat (TPR) protein, with the Tup1 repressor. We have previously shown that the N-terminal domain of Ssn6 comprising TPRs 1 to 3 is necessary and sufficient for this interaction and that TPR1 plays critical role. In a subsequent study, we provided evidence that in the absence of Tup1, TPR1 is susceptible to proteolysis and that conformational change(s) accompany the Ssn6-Tup1 complex formation...
2017: PloS One
https://www.readbyqxmd.com/read/29053450/two-dimensional-isoelectric-focusing-offgel-micro-fluidic-lab-on-chip-electrophoresis-and-ftir-for-assessment-of-long-term-stability-of-rhg-csf-formulation
#2
H Kraiem, F Zouari, R Ben Abderrazek, Y Manon, M El Ayeb, L Fillaudeau, J Bedoui, B Bouhaouala-Zahar
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) has been increasingly recognized from among one of the most abundant families of biosimilars. Upon long-term storage, rhG-CSF is subject to subtle chemical modifications that rapidly occur and, in particular, produce deaminated variants with divergent charge. Indeed, changes in charge from glutamine deamination may alter the way rhG-SCF will refold and the structure of resulting molecule. To assess this charge heterogeneity, 2D gel electrophoresis has limited application...
October 16, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/29052795/effects-of-glutamine-taurine-and-their-association-on-inflammatory-pathway-markers-in-macrophages
#3
Talita Sartori, Guilherme Galvão Dos Santos, Amanda Nogueira-Pedro, Edson Makiyama, Marcelo Macedo Rogero, Primavera Borelli, Ricardo Ambrósio Fock
The immune system is essential for the control and elimination of infections, and macrophages are cells that act as important players in orchestrating the various parts of the inflammatory/immune response. Amino acids play important role in mediating functionality of the inflammatory response, especially mediating macrophages functions and cytokines production. We investigated the influence of glutamine, taurine and their association on the modulation of inflammatory pathway markers in macrophages. The RAW 264...
October 20, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/29051667/free-amino-acids-profile-of-polish-and-slovak-honeys-based-on-lc-ms-ms-method-without-the-prior-derivatisation
#4
Stanisław Kowalski, Mária Kopuncová, Zuzana Ciesarová, Kristína Kukurová
LC-MS/MS method was applied for determination of free amino acids in honey without derivatisation steps. Twenty free amino acids including aspartic acid, asparagine, glutamic acid, glutamine, alanine, arginine, glycine, leucine, histidine, hydroxyproline, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine and ornithine were analyzed in thirty honey samples from Poland and Slovakia. The analysis covered: acacia, lime, rape, multifloral and forest types of honey. Applied method was characterized to had good sensitivity with limit of detection ranged from 3...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29051512/effect-of-specific-mutations-in-cd300-complexes-formation-potential-implication-of-cd300f-in-multiple-sclerosis
#5
Águeda Martínez-Barriocanal, Andrea Arcas-García, Miriam Magallon-Lorenz, Aroa Ejarque-Ortíz, María Luciana Negro-Demontel, Emma Comas-Casellas, Simo Schwartz, Sunny Malhotra, Xavier Montalban, Hugo Peluffo, Margarita Martín, Manuel Comabella, Joan Sayós
Herein, we have used bioinformatics tools to predict five clusters defining ligand-binding sites on the extracellular domain of human CD300b receptor, presumably involved in the formation of both homodimers and heterodimers with other CD300 family members. Site-directed mutagenesis revealed residues glutamic acid 28 and glutamine 29 in cluster 5 to be necessary for the formation of CD300b complexes. Surprisingly, the disruption of cluster 2 and 4 reconstituted the binding capability lost by the mutation of residues glutamic acid 28 to alanine, glutamine 29 to alanine (E28A-Q29G)...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29049903/glutamate-homeostasis-in-the-adult-rat-prefrontal-cortex-is-altered-by-cortical-docosahexaenoic-acid-accrual-during-adolescence-an-in-vivo-1-h-mrs-study
#6
Robert K McNamara, Ruth H Asch, Jennifer D Schurdak, Diana M Lindquist
Major psychiatric disorders are associated with dysregulated glutamate homeostasis and deficits in the omega-3 fatty acid docosahexaenoic acid (DHA). This study determined the effects of dietary-induced alterations in brain DHA accrual on cortical glutamate homeostasis in the adult rat brain. Adolescent rats were fed a control diet (n = 20), a n-3 fatty acid-deficient diet (DEF, n = 20), or a fish oil-fortified diet containing preformed DHA (FO, n = 20). In adulthood (1)H MRS scans were performed with voxels in the prefrontal cortex (PFC) and thalamus...
October 11, 2017: Psychiatry Research
https://www.readbyqxmd.com/read/29047383/a-novel-small-molecule-inhibitor-of-p32-mitochondrial-protein-overexpressed-in-glioma
#7
Venkata Yenugonda, Natsuko Nomura, Valentina Kouznetsova, Igor Tsigelny, Valentina Fogal, Elmar Nurmemmedov, Santosh Kesari, Ivan Babic
BACKGROUND: The mitochondrial protein p32 is a validated therapeutic target of cancer overexpressed in glioma. Therapeutic targeting of p32 with monoclonal antibody or p32-binding LyP-1 tumor-homing peptide can limit tumor growth. However, these agents do not specifically target mitochondrial-localized p32 and would not readily cross the blood-brain barrier to target p32-overexpressing gliomas. Identifying small molecule inhibitors of p32 overexpressed in cancer is a more rational therapeutic strategy...
October 18, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29047296/in%C3%A2-vivo-13-c-mrs-in-the-mouse-brain-at-14-1-tesla-and-metabolic-flux-quantification-under-infusion-of-1-6-13-c2-glucose
#8
Marta Lai, Bernard Lanz, Carole Poitry-Yamate, Jackeline F Romero, Corina M Berset, Cristina Cudalbu, Rolf Gruetter
In vivo (13)C magnetic resonance spectroscopy (MRS) enables the investigation of cerebral metabolic compartmentation while, e.g. infusing (13)C-labeled glucose. Metabolic flux analysis of (13)C turnover previously yielded quantitative information of glutamate and glutamine metabolism in humans and rats, while the application to in vivo mouse brain remains exceedingly challenging. In the present study, (13)C direct detection at 14.1 T provided highly resolved in vivo spectra of the mouse brain while infusing [1,6-(13)C2]glucose for up to 5 h...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29046333/the-p53-inducible-long-noncoding-rna-trings-protects-cancer-cells-from-necrosis-under-glucose%C3%A2-starvation
#9
Muhammad Riaz Khan, Shaoxun Xiang, Zhiyin Song, Mian Wu
The tumor suppressor p53 is activated in response to cellular stress to prevent malignant transformation. However, several recent studies have shown that p53 can play protective roles in tumor cell survival under adversity. Whether p53-regulated long noncoding RNAs are involved in this process remains to be fully understood. Here, we show that under glucose starvation condition, p53 directly upregulates a novel lncRNA named TRINGS (Tp53-regulated inhibitor of necrosis under glucose starvation) in human tumor cells...
October 18, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29046316/evidence-for-the-presence-in-rainbow-trout-brain-of-amino-acid-sensing-systems-involved-in-the-control-of-food-intake
#10
Sara Comesana, Cristina Velasco, Rosa M Ceinos, Marcos A Lopez-Patiño, Jesus M Miguez, Sofia Morais, Jose L Soengas
To assess the existence of central amino acid sensing systems in fish we carried out two experiments in rainbow trout. In the first one, we injected ICV two different branched-chain amino acids (BCAA), leucine and valine, and assessed food intake up to 48 h later. Leucine decreased and valine increased food intake. In a second experiment, 6h after similar ICV treatment we determined changes in parameters related to putative amino acid sensing systems. Amino acid sensing systems respond to leucine in hypothalamus and telencephalon, and to valine in telencephalon...
October 18, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29045397/glucose-feeds-the-tca-cycle-via-circulating-lactate
#11
Sheng Hui, Jonathan M Ghergurovich, Raphael J Morscher, Cholsoon Jang, Xin Teng, Wenyun Lu, Lourdes A Esparza, Tannishtha Reya, Le Zhan, Jessie Yanxiang Guo, Eileen White, Joshua D Rabinowitz
Mammalian tissues are fuelled by circulating nutrients, including glucose, amino acids, and various intermediary metabolites. Under aerobic conditions, glucose is generally assumed to be burned fully by tissues via the tricarboxylic acid cycle (TCA cycle) to carbon dioxide. Alternatively, glucose can be catabolized anaerobically via glycolysis to lactate, which is itself also a potential nutrient for tissues and tumours. The quantitative relevance of circulating lactate or other metabolic intermediates as fuels remains unclear...
October 18, 2017: Nature
https://www.readbyqxmd.com/read/29044214/limits-of-aerobic-metabolism-in-cancer-cells
#12
Jorge Fernandez-de-Cossio-Diaz, Alexei Vazquez
Cancer cells exhibit high rates of glycolysis and glutaminolysis. Glycolysis can provide energy and glutaminolysis can provide carbon for anaplerosis and reductive carboxylation to citrate. However, all these metabolic requirements could be in principle satisfied from glucose. Here we investigate why cancer cells do not satisfy their metabolic demands using aerobic biosynthesis from glucose. Based on the typical composition of a mammalian cell we quantify the energy demand and the OxPhos burden of cell biosynthesis from glucose...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042482/resveratrol-stimulates-the-metabolic-reprogramming-of-human-cd4-t-cells-to-enhance-effector-function
#13
Marco Craveiro, Gaspard Cretenet, Cédric Mongellaz, Maria I Matias, Olivier Caron, Maria C Pedroso de Lima, Valérie S Zimmermann, Eric Solary, Valérie Dardalhon, Vjekoslav Dulić, Naomi Taylor
The polyphenol resveratrol activates the deacetylase Sirt1, resulting in various antioxidant, chemoprotectant, neuroprotective, cardioprotective, and anti-inflammatory properties. We found that at high concentrations of resveratrol, human CD4(+) T cells showed defective antigen receptor signaling and arrest at the G1 stage of the cell cycle, whereas at low concentrations, cells were readily activated and exhibited enhanced Sirt1 deacetylase activity. Nevertheless, low-dose resveratrol rapidly stimulated genotoxic stress in the T cells, which resulted in engagement of a DNA damage response pathway that depended on the kinase ATR [ataxia telangiectasia-mutated (ATM) and Rad3-related], but not ATM, and subsequently in premitotic cell cycle arrest...
October 17, 2017: Science Signaling
https://www.readbyqxmd.com/read/29042306/cigarette-smoke-induces-mitochondrial-metabolic-reprogramming-in-lung-cells
#14
Hitendra S Solanki, Niraj Babu, Ankit Jain, Mohd Younis Bhat, Vinuth N Puttamallesh, Jayshree Advani, Remya Raja, Kiran K Mangalaparthi, Mahesh M Kumar, T S Keshava Prasad, Premendu Prakash Mathur, David Sidransky, Harsha Gowda, Aditi Chatterjee
Cellular transformation owing to cigarette smoking is due to chronic exposure and not acute. However, systematic studies to understand the molecular alterations in lung cells due to cigarette smoke are lacking. To understand these molecular alterations induced by chronic cigarette smoke exposure, we carried out tandem mass tag (TMT) based temporal proteomic profiling of lung cells exposed to cigarette smoke upto 12months. We identified 2620 proteins in total, of which 671 proteins were differentially expressed (1...
October 14, 2017: Mitochondrion
https://www.readbyqxmd.com/read/29040286/human-babesiosis-indication-of-a-molecular-mimicry-between-thrombospondin-domains-from-a-novel-babesia-microti-bmp53-protein-and-host-platelets-molecules
#15
Ahmed Abdelmoniem Mousa, Daniel Barry Roche, Mohamad Alaa Terkawi, Kyohko Kameyama, Ketsarin Kamyingkird, Patrick Vudriko, Akram Salama, Shinuo Cao, Sahar Orabi, Hanem Khalifa, Mohamed Ahmed, Mabrouk Attia, Ahmed Elkirdasy, Yoshifumi Nishikawa, Xuenan Xuan, Emmanuel Cornillot
Human babesiosis is caused by the apicomplexan parasite Babesia microti, which is of major public health concern in the United States and elsewhere, resulting in malaise and fatigue, followed by a fever and hemolytic anemia. In this paper we focus on the characterization of a novel B. microti thrombospondin domain (TSP1)-containing protein (BmP53) from the new annotation of the B. microti genome (locus 'BmR1_04g09041'). This novel protein (BmP53) had a single TSP1 and a transmembrane domain, with a short cytoplasmic tail containing a sub-terminal glutamine residue, but no signal peptide and Von Willebrand factor type A domains (VWA), which are found in classical thrombospondin-related adhesive proteins (TRAP)...
2017: PloS One
https://www.readbyqxmd.com/read/29039929/mechanistic-basis-for-atp-dependent-inhibition-of-glutamine-synthetase-by-tabtoxinine-%C3%AE-lactam
#16
Garrett Patrick, Luting Fang, Jacob Schaefer, Sukrit Singh, Gregory Bowman, Timothy Adam Wencewicz
Tabtoxinine-β-lactam (TβL), also known as wildfire toxin, is a time- and ATP-dependent inhibitor of glutamine synthetase produced by plant pathogenic strains of Pseudomonas syringae. Here we demonstrate that recombinant glutamine synthetase from E. coli phosphorylates the C3-hydroxyl group of the TβL 3-(R)-hydroxy-β-lactam (3-HβL) warhead. Phosphorylation of TβL generates a stable, non-covalent enzyme-ADP-inhibitor complex that resembles the glutamine synthetase tetrahedral transition state. The TβL β-lactam ring remains intact during enzyme inhibition making TβL mechanistically distinct from traditional β-lactam antibiotics such as penicillin...
October 17, 2017: Biochemistry
https://www.readbyqxmd.com/read/29038292/proteolysis-suppresses-spontaneous-prion-generation-in-yeast
#17
Atsushi Okamoto, Nao Hosoda, Anri Tanaka, Gary P Newnam, Yury O Chernoff, Shin-Ichi Hoshino
Prions are infectious proteins that cause fatal neurodegenerative disorders including Creutzfeldt-Jacob and bovine spongiform encephalopathy (mad cow) diseases. The yeast [PSI+] prion is formed by the translation-termination factor Sup35, is the best-studied prion, and provides a useful model system for studying such diseases. However, despite recent progress in the understanding of prion diseases, the cellular defense mechanism against prions has not been elucidated. Here, we report that proteolytic cleavage of Sup35 suppresses spontaneous de novo generation of the [PSI+] prion...
October 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29038291/cystine-uptake-through-the-cystine-glutamate-antiporter-xct-triggers-glioblastoma-cell-death-under-glucose-deprivation
#18
Takeo Goji, Kazuhiko Takahara, Manabu Negishi, Hironori Katoh
Oncogenic signaling in cancer cells alters glucose uptake and utilization to supply sufficient energy and biosynthetic intermediates for survival and sustained proliferation. Oncogenic signaling also prevents oxidative stress and cell death caused by increased production of reactive oxygen species (ROS). However, elevated glucose metabolism in cancer cells, especially in glioblastoma, results in the cells becoming sensitive to glucose deprivation (i.e. in high glucose dependence), which rapidly induces cell death...
October 16, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29037738/peptides-derived-from-in-vitro-gastrointestinal-digestion-of-germinated-soybean-proteins-inhibit-human-colon-cancer-cells-proliferation-and-inflammation
#19
Marcela González-Montoya, Blanca Hernández-Ledesma, Jose Manuel Silván, Rosalva Mora-Escobedo, Cristina Martínez-Villaluenga
The aim was to investigate the potential of germinated soybean proteins asa source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions>10, 5-10, and<5kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264...
March 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29036718/glutamatergic-response-to-heat-pain-stress-in-schizophrenia
#20
Joshua Chiappelli, Qiaoyun Shi, Sarah Andrea Wijtenburg, Raimi Quiton, Krista Wisner, Frank Gaston, Priyadurga Kodi, Christopher Gaudiot, Peter Kochunov, Laura M Rowland, Liyi Elliot Hong
Regulation of stress response involves top-down mechanisms of the frontal-limbic glutamatergic system. As schizophrenia is associated with glutamatergic abnormalities, we hypothesized that schizophrenia patients may have abnormal glutamatergic reactivity within the dorsal anterior cingulate cortex (dACC), a key region involved in perception of and reaction to stress. To test this, we developed a somatic stress paradigm involving pseudorandom application of safe but painfully hot stimuli to the forearm of participants while they were undergoing serial proton magnetic resonance spectroscopy to measure changes in glutamate and glutamine levels in the dACC...
September 23, 2017: Schizophrenia Bulletin
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