keyword
https://read.qxmd.com/read/37345883/molecular-functions-of-mrhl-lncrna-in-mouse-spermatogenesis
#21
REVIEW
Bhavana Kayyar, Shubhangini Kataruka, Vijay Suresh Akhade, M R S Rao
Proliferation and meiotic division of spermatogonial stem cells are highly regulated biological processes that occur during spermatogenesis. In addition to protein coding genes, mammalian genome encodes thousands of lncRNAs which are spatio-temporally expressed and play key role(s) in cellular differentiation and development. Mrhl lncRNA is one such mono-exonic polyadenylated non-coding RNA encoded within the 15th intron of the mouse Phkb gene. Mrhl lncRNA is expressed in mouse testis amongst other tissues...
June 1, 2023: Reproduction
https://read.qxmd.com/read/37249871/rna-interference-as-a-method-of-gene-knockdown-in-cultured-spermatogonia
#22
JOURNAL ARTICLE
Connor Cason, Tessa Lord
Maintenance and self-renewal of the spermatogonial stem cell (SSC) population in the testis are dictated by the expression of a unique suite of genes. In manipulating gene expression through loss-of-function approaches, we can identify important regulatory mechanisms that dictate spermatogonial fate decisions. One such approach is RNA interference (RNAi), which uses natural cellular responses to small interfering RNAs to decrease levels of a targeted transcript. RNAi is performed in primary cultures of undifferentiated spermatogonia, and can be paired with techniques such as spermatogonial transplantation to assess the functional consequences of downregulated expression of the target gene on stem cell maintenance...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/37249865/single-molecule-fluorescence-in-situ-hybridization-for-spatial-detection-of-mrnas-in-sections-of-mammalian-testes
#23
JOURNAL ARTICLE
Victoria D Diaz, Brian P Hermann
Single-molecule fluorescence in situ hybridization (smFISH) enables the detection and localization of individual mRNAs in tissue sections with single-molecule resolution while preserving spatial context, and thus, is a useful tool for examining gene expression in biological systems. In particular, the growing reliance on single-cell RNA sequencing (scRNA-seq) to explore cellular heterogeneity has reinvigorated this approach as a validation tool to spatially re-map mRNA expression patterns described in isolated cells to their parent tissue...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/37068347/tissue-specific-expansion-of-zika-virus-isogenic-variants-drive-disease-pathogenesis
#24
JOURNAL ARTICLE
Kitti Wing Ki Chan, Amanda Makha Bifani, Satoru Watanabe, Milly M Choy, Eng Eong Ooi, Subhash G Vasudevan
BACKGROUND: The Asian lineage Zika virus (ZIKV) emerged as a public health emergency in 2016 causing severe neurological pathologies with no apparent historical correlate to the mild, disease-causing innocuous member of the mosquito-borne flavivirus genus that was discovered in Africa in 1947. Replication error rate of RNA viruses combined with viral protein/RNA structural plasticity can lead to evolution of virus-induced pathogenicity that is critical to identify and validate. METHODS: Infection studies in cells and A129 interferon alpha/beta receptor deficient mice with ZIKV French Polynesian H/PF/2013 clinical isolate, plaque-purified isogenic clone derivatives as well as infectious cDNA clone derived wild-type and site-specific mutant viruses, were employed together with Next-Generation Sequencing (NGS) to pin-point the contributions of specific viral variants in neurovirulence recapitulated in our ZIKV mouse model...
April 15, 2023: EBioMedicine
https://read.qxmd.com/read/37065848/spermiogenesis-alterations-in-the-absence-of-ctcf-revealed-by-single-cell-rna-sequencing
#25
JOURNAL ARTICLE
Ulises Torres-Flores, Fernanda Díaz-Espinosa, Tayde López-Santaella, Rosa Rebollar-Vega, Aarón Vázquez-Jiménez, Ian J Taylor, Rosario Ortiz-Hernández, Olga M Echeverría, Gerardo H Vázquez-Nin, María Concepción Gutierrez-Ruiz, Inti Alberto De la Rosa-Velázquez, Osbaldo Resendis-Antonio, Abrahan Hernández-Hernandez
CTCF is an architectonic protein that organizes the genome inside the nucleus in almost all eukaryotic cells. There is evidence that CTCF plays a critical role during spermatogenesis as its depletion produces abnormal sperm and infertility. However, defects produced by its depletion throughout spermatogenesis have not been fully characterized. In this work, we performed single cell RNA sequencing in spermatogenic cells with and without CTCF. We uncovered defects in transcriptional programs that explain the severity of the damage in the produced sperm...
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37039784/disrupted-intercellular-bridges-and-spermatogenesis-in-fatty-acyl-coa-reductase-1-knockout-mice-a-new-model-of%C3%A2-ether-lipid-deficiency
#26
JOURNAL ARTICLE
Bo Pan, Shuo Yuan, Linda Mayernik, Yi Tian Yap, Kamiar Moin, Charles S Chung, Krishnarao Maddipati, Stephen A Krawetz, Zhibing Zhang, Rex A Hess, Xuequn Chen
Peroxisomal fatty acyl-CoA reductase 1 (FAR1) is a rate-limiting enzyme for ether lipid (EL) synthesis. Gene mutations in FAR1 cause a rare human disease. Furthermore, altered EL homeostasis has also been associated with various prevalent human diseases. Despite their importance in human health, the exact cellular functions of FAR1 and EL are not well-understood. Here, we report the generation and initial characterization of the first Far1 knockout (KO) mouse model. Far1 KO mice were subviable and displayed growth retardation...
May 2023: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/36975404/an-ancient-testis-specific-iq-motif-containing-h-gene-regulates-specific-transcript-isoform-expression-during-spermatogenesis
#27
JOURNAL ARTICLE
Paula Navarrete-López, Marta Lombó, Maria Maroto, Eva Pericuesta, Raúl Fernández-González, Priscila Ramos-Ibeas, María Teresa Parra, Alberto Viera, José Ángel Suja, Alfonso Gutiérrez-Adán
Spermatogenic cells express more alternatively spliced RNAs than most whole tissues; however, the regulation of these events remains unclear. Here we characterize the function of a testis-specific IQ motif containing H gene (Iqch) using a mutant mouse model. We found that Iqch is essential for the specific expression of RNA isoforms during spermatogenesis. Through immunohistochemistry of testis, we noted that Iqch was expressed mainly in the nucleus of spermatocyte and spermatid, where IQCH appeared juxtaposed with SRRM2 and ERSP1 in the nuclear speckles, suggesting that interactions among these proteins regulate alternative splicing (AS)...
March 28, 2023: Development
https://read.qxmd.com/read/36902187/conserved-transcriptome-features-define-prepubertal-primate-spermatogonial-stem-cells-as-a-dark-spermatogonia-and-identify-unique-regulators
#28
JOURNAL ARTICLE
Anukriti Singh, Brian P Hermann
Antineoplastic treatments for cancer and other non-malignant disorders can result in long-term or permanent male infertility by ablating spermatogonial stem cells (SSCs). SSC transplantation using testicular tissue harvested before a sterilizing treatment is a promising approach for restoring male fertility in these cases, but a lack of exclusive biomarkers to unequivocally identify prepubertal SSCs limits their therapeutic potential. To address this, we performed single-cell RNA-seq on testis cells from immature baboons and macaques and compared these cells with published data from prepubertal human testis cells and functionally-defined mouse SSCs...
March 1, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36899892/mirna-expression-profiles-of-mouse-round-spermatids-in-grth-ddx25-mediated-spermiogenesis-mrna-mirna-network-analysis
#29
JOURNAL ARTICLE
Rajakumar Anbazhagan, Raghuveer Kavarthapu, Ryan Dale, Kiersten Campbell, Fabio R Faucz, Maria L Dufau
GRTH/DDX25 is a testis-specific DEAD-box family of RNA helicase, which plays an essential role in spermatogenesis and male fertility. There are two forms of GRTH, a 56 kDa non-phosphorylated form and a 61 kDa phosphorylated form (pGRTH). GRTH-KO and GRTH Knock-In (KI) mice with R242H mutation (lack pGRTH) are sterile with a spermatogenic arrest at step 8 of spermiogenesis due to failure of round spermatids (RS) to elongate. We performed mRNA-seq and miRNA-seq analysis on RS of WT, KI, and KO to identify crucial microRNAs (miRNAs) and mRNAs during RS development by establishing a miRNA-mRNA network...
February 27, 2023: Cells
https://read.qxmd.com/read/36865339/the-pluripotency-factor-tex10-finetunes-wnt-signaling-for-pgc-and-male-germline-development
#30
Dan Li, Jihong Yang, Fanglin Ma, Vikas Malik, Ruge Zang, Xianle Shi, Xin Huang, Hongwei Zhou, Jianlong Wang
Testis-specific transcript 10 (Tex10) is a critical factor for pluripotent stem cell maintenance and preimplantation development. Here, we dissect its late developmental roles in primordial germ cell (PGC) specification and spermatogenesis using cellular and animal models. We discover that Tex10 binds the Wnt negative regulator genes, marked by H3K4me3, at the PGC-like cell (PGCLC) stage in restraining Wnt signaling. Depletion and overexpression of Tex10 hyperactivate and attenuate the Wnt signaling, resulting in compromised and enhanced PGCLC specification efficiency, respectively...
February 24, 2023: bioRxiv
https://read.qxmd.com/read/36860030/tributyltin-chloride-tbtcl-induces-cell-injury-via-dysregulation-of-endoplasmic-reticulum-stress-and-autophagy-in-leydig-cells
#31
JOURNAL ARTICLE
Pengchen Chen, Yali Song, Li Tang, Wenbin Zhong, JingJing Zhang, Min Cao, Junhui Chen, Guangqing Cheng, Huiying Li, Tianyun Fan, Hang Fai Kwok, Jigang Wang, Chuanbin Yang, Wei Xiao
Tributyltin chloride (TBTCL), a commonly used antiseptic substance, is commonly found in the environment. Human exposure to TBTCL through the consumption of contaminated seafood, fish, or drinking water has aroused concern. It is well-characterized that TBTCL has multiple detrimental effects on the male reproductive system. However, the potential cellular mechanisms are not fully elucidated. Here, we characterized molecular mechanisms of TBTCL-induced cell injury in Leydig cells, a critical supporter for spermatogenesis...
April 15, 2023: Journal of Hazardous Materials
https://read.qxmd.com/read/36803965/hypoxia-induces-alterations-in-trna-modifications-involved-in-translational-control
#32
JOURNAL ARTICLE
Huanping Guo, Lin Xia, Wei Wang, Wei Xu, Xipeng Shen, Xiao Wu, Tong He, Xuelin Jiang, Yinying Xu, Pan Zhao, Dongmei Tan, Xi Zhang, Yunfang Zhang
BACKGROUND: Adaptation to high-altitude hypobaric hypoxia has been shown to require a set of physiological traits enabled by an associated set of genetic modifications, as well as transcriptome regulation. These lead to both lifetime adaptation of individuals to hypoxia at high altitudes and generational evolution of populations as seen for instance in those of Tibet. Additionally, RNA modifications, which are sensitive to environmental exposure, have been shown to play pivotal biological roles in maintaining the physiological functions of organs...
February 21, 2023: BMC Biology
https://read.qxmd.com/read/36796361/deficiency-of-primate-specific-ssx1-induced-asthenoteratozoospermia-in-infertile-men-and-cynomolgus-monkey-and-tree-shrew-models
#33
JOURNAL ARTICLE
Chunyu Liu, Wei Si, Chaofeng Tu, Shixiong Tian, Xiaojin He, Shengnan Wang, Xiaoyu Yang, Chencheng Yao, Cong Li, Zine-Eddine Kherraf, Maosen Ye, Zixue Zhou, Yuhua Ma, Yang Gao, Yu Li, Qiwei Liu, Shuyan Tang, Jiaxiong Wang, Hexige Saiyin, Liangyu Zhao, Liqun Yang, Lanlan Meng, Bingbing Chen, Dongdong Tang, Yiling Zhou, Huan Wu, Mingrong Lv, Chen Tan, Ge Lin, Qingpeng Kong, Hong Shi, Zhixi Su, Zheng Li, Yong-Gang Yao, Li Jin, Ping Zheng, Pierre F Ray, Yue-Qiu Tan, Yunxia Cao, Feng Zhang
Primate-specific genes (PSGs) tend to be expressed in the brain and testis. This phenomenon is consistent with brain evolution in primates but is seemingly contradictory to the similarity of spermatogenesis among mammals. Here, using whole-exome sequencing, we identified deleterious variants of X-linked SSX1 in six unrelated men with asthenoteratozoospermia. SSX1 is a PSG expressed predominantly in the testis, and the SSX family evolutionarily expanded independently in rodents and primates. As the mouse model could not be used for studying SSX1, we used a non-human primate model and tree shrews, which are phylogenetically similar to primates, to knock down (KD) Ssx1 expression in the testes...
March 2, 2023: American Journal of Human Genetics
https://read.qxmd.com/read/36793255/transcriptional-regulation-of-spatiotemporal-gene-expression-within-the-seminiferous-epithelium-mouse-acrv1-gene-as-a-model
#34
REVIEW
Prabhakara P Reddi
Precise spatiotemporal expression of cohorts of differentiation markers unique to spermatogonia, spermatocytes, and round spermatids punctuates spermatogenesis and ensures its completion. For example, genes coding for the synaptonemal complex or the acrosome or flagellum are expressed sequentially in a developmental stage- and germ cell-specific manner. But the transcriptional mechanisms governing the spatiotemporal order of gene expression within the seminiferous epithelium are poorly understood. Using the round spermatid-specific Acrv1 gene, which codes for the acrosomal protein SP-10 as a model, we learned that 1) the proximal promoter itself contains all the necessary cis-regulatory sequences, 2) an insulator prevents somatic cell expression of the testis-specific gene, 3) RNA II polymerase is loaded on the Acrv1 promoter but paused in spermatocytes, thus ensuring precise transcriptional elongation in round spermatids, and that 4) a transcriptional repressor TDP-43 plays a role maintaining the paused state in spermatocytes...
February 15, 2023: Andrology
https://read.qxmd.com/read/36726594/overview-of-single-cell-rna-sequencing-analysis-and-its-application-to-spermatogenesis-research
#35
REVIEW
Takahiro Suzuki
BACKGROUND: Single-cell transcriptomics allows parallel analysis of multiple cell types in tissues. Because testes comprise somatic cells and germ cells at various stages of spermatogenesis, single-cell RNA sequencing is a powerful tool for investigating the complex process of spermatogenesis. However, single-cell RNA sequencing analysis needs extensive knowledge of experimental technologies and bioinformatics, making it difficult for many, particularly experimental biologists and clinicians, to use it...
2023: Reproductive Medicine and Biology
https://read.qxmd.com/read/36721956/persistence-of-foetal-testicular-features-in-patients-with-defective-androgen-signalling
#36
JOURNAL ARTICLE
Mostafa Al-Sharkawi, Verónica Calonga-Solís, Franz F Dressler, Hauke Busch, Olaf Hiort, Ralf Werner
OBJECTIVE: Congenital defects of androgen synthesis or action in 46,XY individuals can result in impaired virilisation, despite the apparent testicular development. In a recent case, report of a young adult with complete androgen insensitivity syndrome (CAIS), tumourous gonadal tissue was shown to express HSD17B3 in Sertoli cells (SCs) and not in Leydig cells (LCs). This expression pattern differs from the typical adult human testis and resembles a foetal mouse testis, suggesting an underlying testicular development and function defect...
January 10, 2023: European Journal of Endocrinology
https://read.qxmd.com/read/36710661/mha-an-interactive-website-for-scrna-seq-data-of-male-genitourinary-development-and-disease
#37
JOURNAL ARTICLE
LiangYu Zhao, YiFan Zhao, ChenCheng Yao, YingBo Dai, Zheng Li, YuXin Tang
BACKGROUND: The development of single-cell sequencing technology has expanded the understanding of cell heterogeneity and disease progression in the male genitourinary system. However, complex processing processes and unprofessional analytical annotations limit the daily use and wildly sharing of published datasets. OBJECTIVES: single-cell sequencing data of male-specific tissues and organs. MATERIALS AND METHODS: The data were downloaded from published studies and were processed based on the Seurat R package, including quality control, cell clustering, reduction and graph generation, and cell type annotation was differentiated by referring to the related paper or recognized cell markers...
January 30, 2023: Andrology
https://read.qxmd.com/read/36698249/adad2-functions-in-spermiogenesis-and-pirna-biogenesis-in-mice
#38
JOURNAL ARTICLE
Yonggang Lu, Ippei Nagamori, Hisato Kobayashi, Kanako Kojima-Kita, Kenjiro Shirane, Hsin-Yi Chang, Toru Nishimura, Takayuki Koyano, Zhifeng Yu, Julio M Castañeda, Makoto Matsuyama, Satomi Kuramochi-Miyagawa, Martin M Matzuk, Masahito Ikawa
BACKGROUND: Adenosine deaminase domain containing 2 (ADAD2) is a testis-specific protein composed of a double-stranded RNA binding domain and a non-catalytic adenosine deaminase domain. A recent study showed that ADAD2 is indispensable for the male reproduction in mice. However, the detailed functions of ADAD2 remain elusive. OBJECTIVES: This study aimed to investigate the cause of male sterility in Adad2 mutant mice and to understand the molecular functions of ADAD2...
May 2023: Andrology
https://read.qxmd.com/read/36692420/allogeneic-car-t-cells-targeting-dll3-are-efficacious-and-safe-in-preclinical-models-of-small-cell-lung-cancer
#39
JOURNAL ARTICLE
Yi Zhang, Silvia K Tacheva-Grigorova, Janette Sutton, Zea Melton, Yvonne S L Mak, Cecilia Lay, Bryan A Smith, Tao Sai, Thomas Van Blarcom, Barbra J Sasu, Siler H Panowski
PURPOSE: Small cell lung cancer (SCLC) is an aggressive disease with limited treatment options. Delta-like ligand 3 (DLL3) is highly expressed on SCLC and several other types of neuroendocrine cancers, with limited normal tissue RNA expression in brain, pituitary, and testis, making it a promising CAR T-cell target for SCLC and other solid tumor indications. EXPERIMENTAL DESIGN: A large panel of anti-DLL3 scFv-based CARs were characterized for both in vitro and in vivo activity...
March 1, 2023: Clinical Cancer Research
https://read.qxmd.com/read/36690000/comparing-the-adult-and-pre-pubertal-testis-metabolic-transitions-and-the-change-in-the-spermatogonial-stem-cell-metabolic-microenvironment
#40
REVIEW
Anna Laura Voigt, Nathalia de Lima E Martins Lara, Ina Dobrinski
BACKGROUND: Survivors of childhood cancer often suffer from infertility. While sperm cryopreservation is not feasible before puberty, the patient's own spermatogonial stem cells could serve as a germ cell reservoir, enabling these patients to father their own children in adulthood through the isolation, in vitro expansion, and subsequent transplantation of spermatogonial stem cells. However, this approach requires large numbers of stem cells, and methods for successfully propagating spermatogonial stem cells in the laboratory are yet to be established for higher mammals and humans...
January 23, 2023: Andrology
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