keyword
MENU ▼
Read by QxMD icon Read
search

mouse testis RNA

keyword
https://www.readbyqxmd.com/read/27895724/expression-profile-of-spaca5-spaca5-in-spermatogenesis-and-transitional-cell-carcinoma-of-the-bladder
#1
Xiao-Yue Zhang, Qiu-Xia Yan, Xiao-Yan Guo, Cai-Rong Chen, Run-Qiang Chen, Zhi-Ming Cai, Ai-Fa Tang
The majority of bladder cancer-associated mortalities are due to transitional cell carcinoma (TCC), which is the most prevalent and chemoresistant malignancy of the bladder. Sperm acrosome associated 5 (SPACA5)/Spaca5 is a sperm acrosome-associated, c-type lysozyme-like protein that has been recently identified, and has been designated as an attractive candidate antigen for cancer testis. In the present study, the expression profile of SPACA5/Spaca5 was analyzed in spermatogenesis and TCC of the bladder using diverse molecular and cellular biology methods...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27856695/multiplex-shrna-screening-of-germ-cell-development-by-in-vivo-transfection-of-mouse-testis
#2
Nicholas R Y Ho, Abul R Usmani, Yan Yin, Liang Ma, Donald F Conrad
Spermatozoa are one of the few mammalian cells types that cannot be fully derived in vitro, severely limiting the application of modern genomic techniques to study germ cell biology. The current gold standard approach of characterizing single gene knockout mice is slow as generation of each mutant line can take 6-9 months. Here, we describe an in vivo approach to rapid functional screening of germline genes based on a new non-surgical, non-viral in vivo transfection method to deliver nucleic acids into testicular germ cells...
November 16, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27780883/human-umbilical-perivascular-cells-a-novel-source-of-mscs-to-support-testicular-niche-regeneration
#3
Leila Maghen, Ekaterina Shlush, Itai Gat, Melissa Filice, Tanya Barretto, Keith Jarvi, Kirk Lo, Andree S Gauthier-Fisher, Clifford L Librach
The expansion of functional testicular biopsy-derived human spermatogonial stem cells (hSSC) ex-vivo may enable the restoration of fertility in pre-pubertal males having undergone gonadotoxic therapies or men with severe male factor infertility. Various somatic cells are known to regulate SSC homeostasis and spermatogenesis in the developing and adult testis. Prior attempts to recapitulate this niche demonstrated the requirement of feeder cells, such as endogenous testicular somatic cells, for germ cell expansion ex-vivo...
October 25, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27734358/identification-of-mouse-pirna-pathway-components-using-anti-miwi2-antibodies
#4
Takamasa Hirano, Hidetoshi Hasuwa, Haruhiko Siomi
The mouse testis has served as a popular model system to study a wide range of biological processes, including germ cell development, meiosis, epigenetic changes of chromatin, transposon silencing, and small RNA-mediated epigenetic modifications. PIWI-interacting RNAs (piRNAs) are a class of small RNAs that are almost exclusively expressed in animal gonads. They repress transposons by forming effector complexes with PIWI proteins to maintain genome integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27733379/tspan8-expression-distinguishes-spermatogonial-stem-cells-in-the-prepubertal-mouse-testis
#5
Kazadi Mutoji, Anukriti Singh, Thu Nguyen, Heidi Gildersleeve, Amy V Kaucher, Melissa J Oatley, Jon M Oatley, Ellen K Velte, Christopher B Geyer, Keren Cheng, John R McCarrey, Brian P Hermann
Precise separation of spermatogonial stem cells (SSCs) from progenitor spermatogonia that lack stem cell activity and are committed to differentiation remains a challenge. To distinguish between these spermatogonial subtypes, we identified genes that exhibited bimodal mRNA levels at the single-cell level among undifferentiated spermatogonia from Postnatal Day 6 mouse testes, including Tspan8, Epha2, and Pvr, each of which encode cell surface proteins useful for cell selection. Transplantation studies provided definitive evidence that a TSPAN8-high subpopulation is enriched for SSCs...
December 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27560004/expression-dynamics-relationships-and-transcriptional-regulations-of-diverse-transcripts-in-mouse-spermatogenic-cells
#6
Xiwen Lin, Miao Han, Lu Cheng, Jian Chen, Zhuqiang Zhang, Ting Shen, Min Wang, Bo Wen, Ting Ni, Chunsheng Han
Among all tissues of the metazoa, the transcritpome of testis displays the highest diversity and specificity. However, its composition and dynamics during spermatogenesis have not been fully understood. Here, we have identified 20,639 message RNAs (mRNAs), 7,168 long non-coding RNAs (lncRNAs) and 15,101 circular RNAs (circRNAs) in mouse spermatogenic cells, and found many of them were specifically expressed in testes. lncRNAs are significantly more testis-specific than mRNAs. At all stages, mRNAs are generally more abundant than lncRNAs, and linear transcripts are more abundant than circRNAs...
October 2, 2016: RNA Biology
https://www.readbyqxmd.com/read/27519264/regulatory-complexity-revealed-by-integrated-cytological-and-rna-seq-analyses-of-meiotic-substages-in-mouse-spermatocytes
#7
Robyn L Ball, Yasuhiro Fujiwara, Fengyun Sun, Jianjun Hu, Matthew A Hibbs, Mary Ann Handel, Gregory W Carter
BACKGROUND: The continuous and non-synchronous nature of postnatal male germ-cell development has impeded stage-specific resolution of molecular events of mammalian meiotic prophase in the testis. Here the juvenile onset of spermatogenesis in mice is analyzed by combining cytological and transcriptomic data in a novel computational analysis that allows decomposition of the transcriptional programs of spermatogonia and meiotic prophase substages. RESULTS: Germ cells from testes of individual mice were obtained at two-day intervals from 8 to 18 days post-partum (dpp), prepared as surface-spread chromatin and immunolabeled for meiotic stage-specific protein markers (STRA8, SYCP3, phosphorylated H2AFX, and HISTH1T)...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27486773/integrated-analysis-mirna-and-mrna-profiling-in-patients-with-severe-oligozoospermia-reveals-mir-34c-3p-downregulates-plcxd3-expression
#8
Zhiming Li, Zaozao Zheng, Jun Ruan, Zhi Li, Xuan Zhuang, Chi-Meng Tzeng
Our previous research suggested that an integrated analysis of microRNA (miRNA) and messenger RNA (mRNA) expression is helpful to explore miRNA-mRNA interactions and to uncover the molecular mechanisms of male infertility. In this study, microarrays were used to compare the differences in the miRNA and mRNA expression profiles in the testicular tissues of severe oligozoospermia (SO) patients with obstructive azoospermia (OA) controls with normal spermatogenesis. Four miRNAs (miR-1246, miR-375, miR-410, and miR-758) and six mRNAs (SLC1A3, PRKAR2B, HYDIN, WDR65, PRDX1, and ADATMS5) were selected to validate the microarray data using quantitative real-time PCR...
July 29, 2016: Oncotarget
https://www.readbyqxmd.com/read/27473657/mouse-d1pas1-a-dead-box-rna-helicase-is-required-for-the-completion-of-first-meiotic-prophase-in-male-germ-cells
#9
Hiroki Inoue, Narumi Ogonuki, Michiko Hirose, Yuki Hatanaka, Shogo Matoba, Shinichiro Chuma, Kimio Kobayashi, Shigeharu Wakana, Junko Noguchi, Kimiko Inoue, Kentaro Tanemura, Atsuo Ogura
D1Pas1 is a mouse autosomal DEAD-box RNA helicase expressed predominantly in the testis. To assess its possible function, we generated D1Pas1-deficient mice using embryonic stem cells with a targeted D1Pas1 allele. Deletion of D1Pas1 did not cause noticeable embryonic defects or death, indicating that D1Pas1 is not essential for embryogenesis. Whereas homozygous knockout female mice showed normal reproductive performance, homozygous knockout male mice were completely sterile. The seminiferous epithelium of D1Pas1-deficient males contained no spermatids or spermatozoa because of spermatogenic arrest at the late pachytene stage...
September 16, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27342271/overexpression-of-stra8-boule-and-dazl-genes-promotes-goat-bone-marrow-derived-mesenchymal-stem-cells-in-vitro-transdifferentiation-toward-putative-male-germ-cells
#10
Pei-Zhen Li, Guang-Yao Yan, Le Han, Jing Pang, Bu-Shuai Zhong, Guo-Min Zhang, Feng Wang, Yan-Li Zhang
Bone marrow mesenchymal stem cells (BMSCs), which are well characterized and widely utilized adult stem cells, encompass the capacity to commit to a variety of cell types. This study was conducted to develop an effective way to induce goat BMSCs (gBMSCs) to transdifferentiate toward putative male germ cells by overexpressing STRA8 (stimulated by RA-8), BOULE (also called BOLL), and DAZL (deleted in azoospermia-like). First, we found that the expression levels of these 3 genes gradually increased during development of the goat testis from 10 days postnatal to 8 months old...
June 23, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27111572/a-genomic-region-transcribed-into-a-long-noncoding-rna-interacts-with-the-prss42-tessp-2-promoter-in-spermatocytes-during-mouse-spermatogenesis-and-its-flanking-sequences-can-function-as-enhancers
#11
Ryoma Yoneda, Yui Satoh, Ikuya Yoshida, Shohei Kawamura, Tomoya Kotani, Atsushi P Kimura
Spermatogenesis is regulated by many meiotic stage-specific genes, but how they coordinate the many individual processes is not fully understood. The Prss/Tessp gene cluster is located on mouse chromosome 9F2-F3, and the three genes at this site (Prss42/Tessp-2, Prss43/Tessp-3, and Prss44/Tessp-4) are specifically activated during meiosis in pachytene spermatocytes. We searched for DNase I hypersensitive sites (HSs) and long noncoding RNAs (lncRNAs) at the Prss/Tessp locus to elucidate how they are activated...
June 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27076447/a-novel-testis-specific-gene-ccdc136-is-required-for-acrosome-formation-and-fertilization-in-mice
#12
Qiang Geng, Liwei Ni, Bin Ouyang, Yanhua Hu, Yu Zhao, Jun Guo
Testis-specific genes are essential for the spermatogenesis in mammalian male reproduction. In this study, we have identified a novel testis-specific gene, Ccdc136 (coiled-coil domain containing 136), from the results of high-throughput gene expression profiling in the developmental stage of mouse testes. Ccdc136 was conserved across species in evolution. Quantitative real-time polymerase chain reaction and Western blot analyses showed that Ccdc136 messenger RNA and protein were extraordinarily expressed in mouse testes, which was first presented at postnatal 3 week and increased in an age-dependent manner before adulthood...
October 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/26963275/a-study-of-differential-expression-of-testicular-genes-in-various-reproductive-phases-of-hemidactylus-flaviviridis-wall-lizard-to-derive-their-association-with-onset-of-spermatogenesis-and-its-relevance-to-mammals
#13
Hironmoy Sarkar, Satyapal Arya, Umesh Rai, Subeer S Majumdar
Testis of Hemidactylus flaviviridis, commonly known as Indian wall lizard, displays a lack of cellular and metabolic activity in regressed phase of testis during non-breeding season of the year. Retracted Sertoli cells (Sc), fibroid myoid cells and pre-meiotic resting spermatogonia are observed in such testis. This situation is akin to certain forms of infertility in men where hormone supplementation fails to generate sperm despite the presence of Sc and germ cells (Gc) in testis. In testis of lizard, spermatogenesis is reinitiated upon increased level of hormones during appropriate season (phase of recrudescence)...
2016: PloS One
https://www.readbyqxmd.com/read/26853561/transcription-factor-rfx2-is-a-key-regulator-of-mouse-spermiogenesis
#14
Yujian Wu, Xiangjing Hu, Zhen Li, Min Wang, Sisi Li, Xiuxia Wang, Xiwen Lin, Shangying Liao, Zhuqiang Zhang, Xue Feng, Si Wang, Xiuhong Cui, Yanling Wang, Fei Gao, Rex A Hess, Chunsheng Han
The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26808486/non-coding-rna-profiling-of-the-developing-murine-lens
#15
Shahid Y Khan, Sean F Hackett, S Amer Riazuddin
Non-coding RNAs (ncRNAs) are emerging as an important player in the regulation of genome integrity and gene expression, and they have been implicated in the pathogenesis of many diseases. The aim of the present study is to identify the repertoire of ncRNAs expressed in the developing mouse lens. We previously reported the mouse lens transcriptome, including mRNA and microRNA (miRNA) profiling at two embryonic (E15 and E18) and four postnatal (P0, P3, P6, and P9) time points. We analyzed the data from small RNA-Seq and mRNA-Seq libraries to investigate the ncRNA profile...
April 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/26801249/alternative-cleavage-and-polyadenylation-in-spermatogenesis-connects-chromatin-regulation-with-post-transcriptional-control
#16
Wencheng Li, Ji Yeon Park, Dinghai Zheng, Mainul Hoque, Ghassan Yehia, Bin Tian
BACKGROUND: Most mammalian genes display alternative cleavage and polyadenylation (APA). Previous studies have indicated preferential expression of APA isoforms with short 3' untranslated regions (3'UTRs) in testes. RESULTS: By deep sequencing of the 3' end region of poly(A) + transcripts, we report widespread shortening of 3'UTR through APA during the first wave of spermatogenesis in mouse, with 3'UTR size being the shortest in spermatids. Using genes without APA as a control, we show that shortening of 3'UTR eliminates destabilizing elements, such as U-rich elements and transposable elements, which appear highly potent during spermatogenesis...
2016: BMC Biology
https://www.readbyqxmd.com/read/26771610/optimizing-a-male-reproductive-aging-mouse-model-by-d-galactose-injection
#17
Chun-Hou Liao, Bing-Huei Chen, Han-Sun Chiang, Chiu-Wei Chen, Mei-Feng Chen, Chih-Chun Ke, Ya-Yun Wang, Wei-Ning Lin, Chi-Chung Wang, Ying-Hung Lin
The d-galactose (d-gal)-injected animal model, which is typically established by administering consecutive subcutaneous d-gal injections to animals for approximately six or eight weeks, has been frequently used for aging research. In addition, this animal model has been demonstrated to accelerate aging in the brain, kidneys, liver and blood cells. However, studies on aging in male reproductive organs that have used this animal model remain few. Therefore, the current study aimed to optimize a model of male reproductive aging by administering d-gal injections to male mice and to determine the possible mechanism expediting senescence processes during spermatogenesis...
January 13, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26720455/exome-sequencing-reveals-agbl5-as-novel-candidate-gene-and-additional-variants-for-retinitis-pigmentosa-in-five-turkish-families
#18
Simone Kastner, Ina-Janine Thiemann, Gabriele Dekomien, Elisabeth Petrasch-Parwez, Sabrina Schreiber, Denis A Akkad, Wanda M Gerding, Sabine Hoffjan, Sezgin Günes, Selçuk Günes, Hasan Bagci, Jörg T Epplen
PURPOSE: Retinitis pigmentosa (RP) is the most common inherited retinal disease with high genetic heterogeneity and variable phenotypes. Characteristic symptoms include night blindness and progressive loss of visual field, leading to blindness. Mutations in >60 genes have been identified to date as causative for RP, and additional candidate genes are assumed. METHODS: To find the disease-causing mutations in the affected members of five Turkish families, we sequenced whole exomes using an Illumina platform...
December 2015: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26713853/specific-tandem-3-utr-patterns-and-gene-expression-profiles-in-mouse-thy1-germline-stem-cells
#19
Yan Huang, Yuanyan Xiong, Zhuoheng Lin, Xuyang Feng, Xue Jiang, Zhou Songyang, Junjiu Huang
A recently developed strategy of sequencing alternative polyadenylation (APA) sites (SAPAS) with second-generation sequencing technology can be used to explore complete genome-wide patterns of tandem APA sites and global gene expression profiles. spermatogonial stem cells (SSCs) maintain long-term reproductive abilities in male mammals. The detailed mechanisms by which SSCs self-renew and generate mature spermatozoa are not clear. To understand the specific alternative polyadenylation pattern and global gene expression profile of male germline stem cells (GSCs, mainly referred to SSCs here), we isolated and purified mouse Thy1+ cells from testis by magnetic-activated cell sorting (MACS) and then used the SAPAS method for analysis, using pluripotent embryonic stem cells (ESCs) and differentiated mouse embryonic fibroblast cells (MEFs) as controls...
2015: PloS One
https://www.readbyqxmd.com/read/26658707/piwil1-is-expressed-in-the-canine-testis-increases-with-sexual-maturity-and-binds-small-rnas
#20
Leanne Stalker, Stewart J Russell, Carmon Co, Robert A Foster, Jonathan LaMarre
Spermatogenesis is a highly regulated process leading to the development of functional spermatozoa through meiotic division and subsequent maturation. Recent studies have suggested that a novel class of Argonaute proteins, known as the PIWI clade, plays important roles in multiple stages of spermatogenesis. PIWI proteins bind specific small noncoding RNAs, called PIWI-interacting RNAs (piRNAs). These piRNAs guide the PIWI-piRNA complex to retrotransposon targets that become expressed during meiosis. Retrotransposons are subsequently silenced, either through PIWI "slicer" activity or through PIWI-directed methylation of the retrotransposon locus...
January 2016: Biology of Reproduction
keyword
keyword
108540
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"