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mouse testis RNA

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https://www.readbyqxmd.com/read/29324788/esrp1-is-a-marker-of-mouse-fetal-germ-cells-and-differentially-expressed-during-spermatogenesis
#1
Shaghayegh Saeidi, Farnaz Shapouri, Robb U de Iongh, Franca Casagranda, Jessie M Sutherland, Patrick S Western, Eileen A McLaughlin, Mary Familari, Gary R Hime
ESRP1 regulates alternative splicing, producing multiple transcripts from its target genes in epithelial tissues. It is upregulated during mesenchymal to epithelial transition associated with reprogramming of fibroblasts to iPS cells and has been linked to pluripotency. Mouse fetal germ cells are the founders of the adult gonadal lineages and we found that Esrp1 mRNA was expressed in both male and female germ cells but not in gonadal somatic cells at various stages of gonadal development (E12.5-E15.5). In the postnatal testis, Esrp1 mRNA was highly expressed in isolated cell preparations enriched for spermatogonia but expressed at lower levels in those enriched for pachytene spermatocytes and round spermatids...
2018: PloS One
https://www.readbyqxmd.com/read/29324782/mafb-is-dispensable-for-the-fetal-testis-morphogenesis-and-the-maintenance-of-spermatogenesis-in-adult-mice
#2
Hossam H Shawki, Hisashi Oishi, Toshiaki Usui, Yu Kitadate, Walaa A Basha, Ahmed M Abdellatif, Kazunori Hasegawa, Risa Okada, Keiji Mochida, Hany A El-Shemy, Masafumi Muratani, Atsuo Ogura, Shosei Yoshida, Satoru Takahashi
The transcription factor MAFB is an important regulator of the development and differentiation of various organs and tissues. Previous studies have shown that MAFB is expressed in embryonic and adult mouse testes and is expected to act as the downstream target of retinoic acid (RA) to initiate spermatogenesis. However, its exact localization and function remain unclear. Here, we localized MAFB expression in embryonic and adult testes and analyzed its gene function using Mafb-deficient mice. We found that MAFB and c-MAF are the only large MAF transcription factors expressed in testes, while MAFA and NRL are not...
2018: PloS One
https://www.readbyqxmd.com/read/29209210/critical-role-of-hepatic-cyp450s-in-the-testis-specific-toxicity-of-5r-5-hydroxytriptolide-in-c57bl-6-mice
#3
Cunzhi Yu, Yu Li, Mingxia Liu, Man Gao, Chenggang Li, Hong Yan, Chunzhu Li, Lihan Sun, Liying Mo, Chunyong Wu, Xinming Qi, Jin Ren
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide (LLDT-8), a novel triptolide derivative immunosuppressant in a conditional knockout (KO) mouse model with liver-specific deletion of CYP450 reductase. Compared to wild type (WT) mice, after LLDT-8 treatment, KO mice suffered severe testicular toxicity (decreased testicular weight, spermatocytes apoptosis) unlike WT mice...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29163367/a-testis-specific-long-non-coding-rna-lncrna-tcam1-regulates-immune-related-genes-in-mouse-male-germ-cells
#4
Misuzu Kurihara, Kai Otsuka, Shin Matsubara, Akira Shiraishi, Honoo Satake, Atsushi P Kimura
Spermatogenesis is precisely controlled by hormones from the hypothalamus-pituitary-gonadal axis and testis-specific genes, but the regulatory mechanism is not fully understood. Recently, a large number of long non-coding RNAs (lncRNAs) are found to be transcribed at each stage of meiosis of male germ cells, and their functions in spermatogenesis have yet to be fully investigated. lncRNA-testicular cell adhesion molecule 1 (lncRNA-Tcam1) is a nuclear lncRNA which is specifically expressed in mouse male germ cells and presumed to play a role in gene regulation during meiosis...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29126923/clock-interacts-with-ranbp9-and-is-involved-in-alternative-splicing-in-spermatogenesis
#5
Junbao Yang, Zhiwei Zhang, Yingying Zhang, Xulei Zheng, Yilu Lu, Dachang Tao, Yunqiang Liu, Yongxin Ma
The core circadian gene CLOCK plays an important role in regulating male reproduction. However, the underlying mechanism still remains unclear. In the present study, we executed yeast two-hybrid screening using cDNA fragment of CLOCK PAS A domain as bait, and identified RANBP9 as a novel protein interacting with CLOCK. The interaction between CLOCK and RANBP9 was further validated by in vivo and in vitro assays. Previous studies have confirmed that SF3B3 was a RANBP9 interacting protein. Subsequently, our study also found that CLOCK and SF3B3 can interact with each other by co-immunoprecipitation in mouse testis...
November 7, 2017: Gene
https://www.readbyqxmd.com/read/29082235/tissue-and-cell-type-specific-expression-of-the-long-noncoding-rna-klhl14-as-in-mouse
#6
Sara Carmela Credendino, Nicole Lewin, Miriane de Oliveira, Swaraj Basu, Barbara D'Andrea, Elena Amendola, Luigi Di Guida, Antonio Nardone, Remo Sanges, Mario De Felice, Gabriella De Vita
lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described...
2017: International Journal of Genomics
https://www.readbyqxmd.com/read/29079676/sex-biased-microrna-expression-in-mammals-and-birds-reveals-underlying-regulatory-mechanisms-and-a-role-in-dosage-compensation
#7
Maria Warnefors, Katharina Mössinger, Jean Halbert, Tania Studer, John L VandeBerg, Isa Lindgren, Amir Fallahshahroudi, Per Jensen, Henrik Kaessmann
Sexual dimorphism depends on sex-biased gene expression, but the contributions of microRNAs (miRNAs) have not been globally assessed. We therefore produced an extensive small RNA sequencing data set to analyze male and female miRNA expression profiles in mouse, opossum, and chicken. Our analyses uncovered numerous cases of somatic sex-biased miRNA expression, with the largest proportion found in the mouse heart and liver. Sex-biased expression is explained by miRNA-specific regulation, including sex-biased chromatin accessibility at promoters, rather than piggybacking of intronic miRNAs on sex-biased protein-coding genes...
October 27, 2017: Genome Research
https://www.readbyqxmd.com/read/29044429/dynamic-expression-of-long-noncoding-rnas-reveals-their-potential-roles-in-spermatogenesis-and-fertility
#8
Lauren Wichman, Saigopal Somasundaram, Christine Breindel, Dana M Valerio, John R McCarrey, Craig A Hodges, Ahmad M Khalil
Mammalian reproduction requires that males and females produce functional haploid germ cells through complex cellular differentiation processes known as spermatogenesis and oogenesis, respectively. While numerous studies have functionally characterized protein-coding genes and small noncoding RNAs (microRNAs and piRNAs) that are essential for gametogenesis, the roles of regulatory long noncoding RNAs (lncRNAs) are yet to be fully characterized. Previously, we and others have demonstrated that intergenic regions of the mammalian genome encode thousands of long noncoding RNAs, and many studies have now demonstrated their critical roles in key biological processes...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28961800/zika-virus-and-reproduction-facts-questions-and-current-management
#9
Sylvie Epelboin, Emmanuel Dulioust, Loïc Epelboin, Alexandra Benachi, Françoise Merlet, Catherine Patrat
BACKGROUND: Zika virus (ZIKV) is an arthropod-borne virus of the family Flaviviridae, genus Flavivirus. ZIKV is currently the focus of an ongoing pandemic and worldwide public health emergency. Although originally isolated in 1947, its pathogenesis was poorly known and very few documented infections were published until recently. Its route of transmission and its impact on reproduction and pregnancy have only recently begun to be disclosed. OBJECTIVE AND RATIONALE: This review summarizes the most recent knowledge about ZIKV infection and pathogenesis and focuses on its impacts on male and female genital tracts, including the risks of sexual transmission and to pregnancy...
November 1, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28884822/rna-binding-protein-musashi-2-regulates-piwil1-and-tbx1-in-mouse-spermatogenesis
#10
Jessie M Sutherland, Alexander P Sobinoff, Barbara A Fraser, Kate A Redgrove, Nicole A Siddall, Peter Koopman, Gary R Hime, Eileen A McLaughlin
RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalisation, identified differential expression and localisation of important regulators of transcription, translation, RNA processing, and spermatogenesis...
September 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28743739/ip6k1-is-essential-for-chromatoid-body-formation-and-temporal-regulation-of-tnp2-and-prm2-expression-in-mouse-spermatids
#11
Aushaq Bashir Malla, Rashna Bhandari
Inositol hexakisphosphate kinases (IP6Ks) are enzymes that synthesise the inositol pyrophosphate 5-IP7, which is known to regulate several physiological processes. Deletion of IP6K1, but not other IP6K isoforms, causes sterility in male mice. Here, we present a detailed investigation of the specific function of IP6K1 in spermatogenesis. Within the mouse testis, IP6K1 is expressed at high levels in late stage pachytene spermatocytes and in round spermatids. We found IP6K1 to be a novel component of the chromatoid body, a cytoplasmic granule in round spermatids composed of RNA and RNA binding proteins, and noted that this structure is absent in Ip6k1(-/-) round spermatids...
July 25, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28729426/glycerol-3-phosphate-acyltransferase-2-is-essential-for-normal-spermatogenesis
#12
Maria B Garcia-Fabiani, Mauro A Montanaro, Pablo Stringa, Ezequiel Lacunza, Elizabeth R Cattaneo, Marianela Santana, Magali Pellon-Maison, Maria R Gonzalez-Baro
Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear...
August 30, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28670489/heat-shock-protein-70-2-hsp70-2-a-novel-cancer-testis-antigen-that-promotes-growth-of-ovarian-cancer
#13
Namita Gupta, Nirmala Jagadish, Avadhesha Surolia, Anil Suri
Heat shock protein 70-2 (HSP70-2) is known to be involved in tumor progression. However, its molecular role and mechanism in epithelial ovarian cancer (EOC) remains unknown. In the present investigation, we examined the role of HSP70-2 in cell cycle, apoptosis and epithelial mesenchymal transition pathways in EOC cells in in vitro and in-vivo xenograft mouse model. To investigate the role of HSP70-2 in ovarian cancer, plasmid driven short hairpin RNA approach was used to examine HSP70-2 gene and protein expression in ovarian cancer cell line A-10 (origin: serous papillary cystadenocarcinoma), Caov-3 (origin: adenocarcinoma) and SKOV3 (origin: adenocarcinoma; derived from metastatic site: ascites) by RT-PCR, quantitative-PCR, immunohistochemistry and Western blotting...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28658256/a-noncoding-rna-containing-a-sine-b1-motif-associates-with-meiotic-metaphase-chromatin-and-has-an-indispensable-function-during-spermatogenesis
#14
Ryusuke Nakajima, Takuya Sato, Takehiko Ogawa, Hideyuki Okano, Toshiaki Noce
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was named R53. In situ hybridization and northern blot analyses revealed that the R53 subfragment RNA bears a B1F motif, is processed from the primary transcript, is expressed in adult testis and is predominantly localized in meiotic metaphase chromatin during spermatogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28646208/a-comprehensive-mouse-transcriptomic-bodymap-across-17-tissues-by-rna-seq
#15
Bin Li, Tao Qing, Jinhang Zhu, Zhuo Wen, Ying Yu, Ryutaro Fukumura, Yuanting Zheng, Yoichi Gondo, Leming Shi
The mouse has been widely used as a model organism for studying human diseases and for evaluating drug safety and efficacy. Many diseases and drug effects exhibit tissue specificity that may be reflected by tissue-specific gene-expression profiles. Here we construct a comprehensive mouse transcriptomic BodyMap across 17 tissues of six-weeks old C57BL/6JJcl mice using RNA-seq. We find different expression patterns between protein-coding and non-coding genes. Liver expressed the least complex transcriptomes, that is, the smallest number of genes detected in liver across all 17 tissues, whereas testis and ovary harbor more complex transcriptomes than other tissues...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28627666/expression-pattern-of-zinc-finger-protein-185-in-mouse-testis-and-its-role-in-regulation-of-testosterone-secretion
#16
Xinguo You, Lu Wei, Shutong Fan, Weiwei Yang, Xiaoying Liu, Guohui Wang, Yi Man, Zhifang Pan, Weiguo Feng
Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in cell proliferation and differentiation. To the best of our knowledge, the association between ZNF185 and male reproduction is unknown. In the present study, the expression and localization of ZNF185 in mouse testis, as well as its role in testosterone secretion, cell cycle progression and apoptosis of mouse Leydig cells were investigated. The results of the immunofluorescence analysis indicated that ZNF185 was highly expressed in Leydig cells of the mouse testis, and primarily localized in the cytoplasm...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28600885/immunolocalization-of-tar-dna-binding-protein-of-43%C3%A2-kda-tdp-43-in-mouse-seminiferous-epithelium
#17
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh M Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P Reddi
TAR DNA-binding protein of 43 kDa (TDP-43) is an evolutionarily conserved, ubiquitously expressed, multi-functional DNA/RNA-binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP-43 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP-43 in the testis. We previously cloned TDP-43 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor and regulates the precise spatiotemporal expression of the Acrv1 gene, which encodes the acrosomal protein SP-10, during spermatogenesis...
August 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28575165/genome-wide-profiling-of-sus-scrofa-circular-rnas-across-nine-organs-and-three-developmental-stages
#18
Guoming Liang, Yalan Yang, Guanglin Niu, Zhonglin Tang, Kui Li
The spatio-temporal expression patterns of Circular RNA (circRNA) across organs and developmental stages are critical for its function and evolution analysis. However, they remain largely unclear in mammals. Here, we comprehensively analysed circRNAs in nine organs and three skeletal muscles of Guizhou miniature pig (S. scrofa), a widely used biomedical model animal. We identified 5,934 circRNAs and analysed their molecular properties, sequence conservation, spatio-temporal expression pattern, potential function, and interaction with miRNAs...
October 1, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28518149/rnf138-deficiency-promotes-apoptosis-of-spermatogonia-in-juvenile-male-mice
#19
Longchang Xu, Yalan Lu, Deqiang Han, Rongyan Yao, Han Wang, Shunshun Zhong, Yanyun Luo, Ruiqin Han, Kai Li, Jun Fu, Shudong Zong, Shiying Miao, Wei Song, Linfang Wang
Spermatogenesis, the process by which haploid sperm cells are produced from a diploid precursor cell, is essential for sexual reproduction. Here, we report that RING-finger protein 138 (Rnf138) is highly expressed in testes, especially in spermatogonia and spermatocytes. The role of Rnf138 in spermatogenesis was examined using a Rnf138-knockout mouse model. Rnf138 deficiency resulted in increased apoptosis in spermatogenic cells, loss of proliferative spermatogonia, delayed development of spermatozoa and impaired fertility...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28472341/in-mammalian-foetal-testes-sox9-regulates-expression-of-its-target-genes-by-binding-to-genomic-regions-with-conserved-signatures
#20
Massilva Rahmoun, Rowena Lavery, Sabine Laurent-Chaballier, Nicolas Bellora, Gayle K Philip, Moïra Rossitto, Aleisha Symon, Eric Pailhoux, Florence Cammas, Jessica Chung, Stefan Bagheri-Fam, Mark Murphy, Vivian Bardwell, David Zarkower, Brigitte Boizet-Bonhoure, Philippe Clair, Vincent R Harley, Francis Poulat
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS)...
July 7, 2017: Nucleic Acids Research
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