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mouse testis RNA

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https://www.readbyqxmd.com/read/29126923/clock-interacts-with-ranbp9-and-is-involved-in-alternative-splicing-in-spermatogenesis
#1
Junbao Yang, Zhiwei Zhang, Yingying Zhang, Xulei Zheng, Yilu Lu, Dachang Tao, Yunqiang Liu, Yongxin Ma
The core circadian gene CLOCK plays an important role in regulating male reproduction. However, the underlying mechanism still remains unclear. In the present study, we executed yeast two-hybrid screening using cDNA fragment of CLOCK PAS A domain as bait, and identified RANBP9 as a novel protein interacting with CLOCK. The interaction between CLOCK and RANBP9 was further validated by in vivo and in vitro assays. Previous studies have confirmed that SF3B3 was a RANBP9 interacting protein. Subsequently, our study also found that CLOCK and SF3B3 can interact with each other by co-immunoprecipitation in mouse testis...
November 7, 2017: Gene
https://www.readbyqxmd.com/read/29082235/tissue-and-cell-type-specific-expression-of-the-long-noncoding-rna-klhl14-as-in-mouse
#2
Sara Carmela Credendino, Nicole Lewin, Miriane de Oliveira, Swaraj Basu, Barbara D'Andrea, Elena Amendola, Luigi Di Guida, Antonio Nardone, Remo Sanges, Mario De Felice, Gabriella De Vita
lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described...
2017: International Journal of Genomics
https://www.readbyqxmd.com/read/29079676/sex-biased-microrna-expression-in-mammals-and-birds-reveals-underlying-regulatory-mechanisms-and-a-role-in-dosage-compensation
#3
Maria Warnefors, Katharina Mössinger, Jean Halbert, Tania Studer, John L VandeBerg, Isa Lindgren, Amir Fallahshahroudi, Per Jensen, Henrik Kaessmann
Sexual dimorphism depends on sex-biased gene expression, but the contributions of microRNAs (miRNAs) have not been globally assessed. We therefore produced an extensive small RNA sequencing data set to analyze male and female miRNA expression profiles in mouse, opossum, and chicken. Our analyses uncovered numerous cases of somatic sex-biased miRNA expression, with the largest proportion found in the mouse heart and liver. Sex-biased expression is explained by miRNA-specific regulation, including sex-biased chromatin accessibility at promoters, rather than piggybacking of intronic miRNAs on sex-biased protein-coding genes...
October 27, 2017: Genome Research
https://www.readbyqxmd.com/read/29044429/dynamic-expression-of-long-noncoding-rnas-reveals-their-potential-roles-in-spermatogenesis-and-fertility
#4
Lauren Wichman, Saigopal Somasundaram, Christine Breindel, Dana M Valerio, John R McCarrey, Craig A Hodges, Ahmad M Khalil
Mammalian reproduction requires that males and females produce functional haploid germ cells through complex cellular differentiation processes known as spermatogenesis and oogenesis, respectively. While numerous studies have functionally characterized protein-coding genes and small noncoding RNAs (microRNAs and piRNAs) that are essential for gametogenesis, the roles of regulatory long noncoding RNAs (lncRNAs) are yet to be fully characterized. Previously, we and others have demonstrated that intergenic regions of the mammalian genome encode thousands of long noncoding RNAs, and many studies have now demonstrated their critical roles in key biological processes...
August 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28961800/zika-virus-and-reproduction-facts-questions-and-current-management
#5
Sylvie Epelboin, Emmanuel Dulioust, Loïc Epelboin, Alexandra Benachi, Françoise Merlet, Catherine Patrat
BACKGROUND: Zika virus (ZIKV) is an arthropod-borne virus of the family Flaviviridae, genus Flavivirus. ZIKV is currently the focus of an ongoing pandemic and worldwide public health emergency. Although originally isolated in 1947, its pathogenesis was poorly known and very few documented infections were published until recently. Its route of transmission and its impact on reproduction and pregnancy have only recently begun to be disclosed. OBJECTIVE AND RATIONALE: This review summarizes the most recent knowledge about ZIKV infection and pathogenesis and focuses on its impacts on male and female genital tracts, including the risks of sexual transmission and to pregnancy...
November 1, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28884822/rna-binding-protein-musashi-2-regulates-piwil1-and-tbx1-in-mouse-spermatogenesis
#6
Jessie M Sutherland, Alexander P Sobinoff, Barbara A Fraser, Kate A Redgrove, Nicole A Siddall, Peter Koopman, Gary R Hime, Eileen A McLaughlin
RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalisation, identified differential expression and localisation of important regulators of transcription, translation, RNA processing, and spermatogenesis...
September 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28743739/ip6k1-is-essential-for-chromatoid-body-formation-and-temporal-regulation-of-tnp2-and-prm2-expression-in-mouse-spermatids
#7
Aushaq Bashir Malla, Rashna Bhandari
Inositol hexakisphosphate kinases (IP6Ks) are enzymes that synthesise the inositol pyrophosphate 5-IP7, which is known to regulate several physiological processes. Deletion of IP6K1, but not other IP6K isoforms, causes sterility in male mice. Here, we present a detailed investigation of the specific function of IP6K1 in spermatogenesis. Within the mouse testis, IP6K1 is expressed at high levels in late stage pachytene spermatocytes and in round spermatids. We found IP6K1 to be a novel component of the chromatoid body, a cytoplasmic granule in round spermatids composed of RNA and RNA binding proteins, and noted that this structure is absent in Ip6k1(-/-) round spermatids...
July 25, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28729426/glycerol-3-phosphate-acyltransferase-2-is-essential-for-normal-spermatogenesis
#8
Maria B Garcia-Fabiani, Mauro A Montanaro, Pablo Stringa, Ezequiel Lacunza, Elizabeth R Cattaneo, Marianela Santana, Magali Pellon-Maison, Maria R Gonzalez-Baro
Glycerol-3-phosphate acyltransferases (GPATs) catalyze the first and rate-limiting step in the de novo glycerolipid synthesis. The GPAT2 isoform differs from the other isoforms because its expression is restricted to male germ cells and cancer cells. It has been recently reported that GPAT2 expression in mouse testis fluctuates during sexual maturation and that it is regulated by epigenetic mechanisms in combination with vitamin A derivatives. Despite progress made in this field, information about GPAT2 role in the developing male germ cells remains unclear...
August 30, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28670489/heat-shock-protein-70-2-hsp70-2-a-novel-cancer-testis-antigen-that-promotes-growth-of-ovarian-cancer
#9
Namita Gupta, Nirmala Jagadish, Avadhesha Surolia, Anil Suri
Heat shock protein 70-2 (HSP70-2) is known to be involved in tumor progression. However, its molecular role and mechanism in epithelial ovarian cancer (EOC) remains unknown. In the present investigation, we examined the role of HSP70-2 in cell cycle, apoptosis and epithelial mesenchymal transition pathways in EOC cells in in vitro and in-vivo xenograft mouse model. To investigate the role of HSP70-2 in ovarian cancer, plasmid driven short hairpin RNA approach was used to examine HSP70-2 gene and protein expression in ovarian cancer cell line A-10 (origin: serous papillary cystadenocarcinoma), Caov-3 (origin: adenocarcinoma) and SKOV3 (origin: adenocarcinoma; derived from metastatic site: ascites) by RT-PCR, quantitative-PCR, immunohistochemistry and Western blotting...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28658256/a-noncoding-rna-containing-a-sine-b1-motif-associates-with-meiotic-metaphase-chromatin-and-has-an-indispensable-function-during-spermatogenesis
#10
Ryusuke Nakajima, Takuya Sato, Takehiko Ogawa, Hideyuki Okano, Toshiaki Noce
A search for early response genes that are activated following germ cell induction from mouse embryonic stem cells in vitro led us to the isolation of a long noncoding RNA that contains a SINE (short interspersed element)-B1F motif that was named R53. In situ hybridization and northern blot analyses revealed that the R53 subfragment RNA bears a B1F motif, is processed from the primary transcript, is expressed in adult testis and is predominantly localized in meiotic metaphase chromatin during spermatogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28646208/a-comprehensive-mouse-transcriptomic-bodymap-across-17-tissues-by-rna-seq
#11
Bin Li, Tao Qing, Jinhang Zhu, Zhuo Wen, Ying Yu, Ryutaro Fukumura, Yuanting Zheng, Yoichi Gondo, Leming Shi
The mouse has been widely used as a model organism for studying human diseases and for evaluating drug safety and efficacy. Many diseases and drug effects exhibit tissue specificity that may be reflected by tissue-specific gene-expression profiles. Here we construct a comprehensive mouse transcriptomic BodyMap across 17 tissues of six-weeks old C57BL/6JJcl mice using RNA-seq. We find different expression patterns between protein-coding and non-coding genes. Liver expressed the least complex transcriptomes, that is, the smallest number of genes detected in liver across all 17 tissues, whereas testis and ovary harbor more complex transcriptomes than other tissues...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28627666/expression-pattern-of-zinc-finger-protein-185-in-mouse-testis-and-its-role-in-regulation-of-testosterone-secretion
#12
Xinguo You, Lu Wei, Shutong Fan, Weiwei Yang, Xiaoying Liu, Guohui Wang, Yi Man, Zhifang Pan, Weiguo Feng
Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in cell proliferation and differentiation. To the best of our knowledge, the association between ZNF185 and male reproduction is unknown. In the present study, the expression and localization of ZNF185 in mouse testis, as well as its role in testosterone secretion, cell cycle progression and apoptosis of mouse Leydig cells were investigated. The results of the immunofluorescence analysis indicated that ZNF185 was highly expressed in Leydig cells of the mouse testis, and primarily localized in the cytoplasm...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28600885/immunolocalization-of-tar-dna-binding-protein-of-43%C3%A2-kda-tdp-43-in-mouse-seminiferous-epithelium
#13
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh M Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P Reddi
TAR DNA-binding protein of 43 kDa (TDP-43) is an evolutionarily conserved, ubiquitously expressed, multi-functional DNA/RNA-binding protein with roles in gene transcription, mRNA splicing, stability, transport, micro RNA biogenesis, and suppression of transposons. Aberrant expression of TDP-43 in testis and sperm was recently shown to be associated with male infertility, which highlights the need to understand better the expression of TDP-43 in the testis. We previously cloned TDP-43 from a mouse testis cDNA library, and showed that it functions as a transcriptional repressor and regulates the precise spatiotemporal expression of the Acrv1 gene, which encodes the acrosomal protein SP-10, during spermatogenesis...
August 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28575165/genome-wide-profiling-of-sus-scrofa-circular-rnas-across-nine-organs-and-three-developmental-stages
#14
Guoming Liang, Yalan Yang, Guanglin Niu, Zhonglin Tang, Kui Li
The spatio-temporal expression patterns of Circular RNA (circRNA) across organs and developmental stages are critical for its function and evolution analysis. However, they remain largely unclear in mammals. Here, we comprehensively analysed circRNAs in nine organs and three skeletal muscles of Guizhou miniature pig (S. scrofa), a widely used biomedical model animal. We identified 5,934 circRNAs and analysed their molecular properties, sequence conservation, spatio-temporal expression pattern, potential function, and interaction with miRNAs...
October 1, 2017: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
https://www.readbyqxmd.com/read/28518149/rnf138-deficiency-promotes-apoptosis-of-spermatogonia-in-juvenile-male-mice
#15
Longchang Xu, Yalan Lu, Deqiang Han, Rongyan Yao, Han Wang, Shunshun Zhong, Yanyun Luo, Ruiqin Han, Kai Li, Jun Fu, Shudong Zong, Shiying Miao, Wei Song, Linfang Wang
Spermatogenesis, the process by which haploid sperm cells are produced from a diploid precursor cell, is essential for sexual reproduction. Here, we report that RING-finger protein 138 (Rnf138) is highly expressed in testes, especially in spermatogonia and spermatocytes. The role of Rnf138 in spermatogenesis was examined using a Rnf138-knockout mouse model. Rnf138 deficiency resulted in increased apoptosis in spermatogenic cells, loss of proliferative spermatogonia, delayed development of spermatozoa and impaired fertility...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28472341/in-mammalian-foetal-testes-sox9-regulates-expression-of-its-target-genes-by-binding-to-genomic-regions-with-conserved-signatures
#16
Massilva Rahmoun, Rowena Lavery, Sabine Laurent-Chaballier, Nicolas Bellora, Gayle K Philip, Moïra Rossitto, Aleisha Symon, Eric Pailhoux, Florence Cammas, Jessica Chung, Stefan Bagheri-Fam, Mark Murphy, Vivian Bardwell, David Zarkower, Brigitte Boizet-Bonhoure, Philippe Clair, Vincent R Harley, Francis Poulat
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS)...
July 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28395340/testicular-adenosine-to-inosine-rna-editing-in-the-mouse-is-mediated-by-adarb1%C3%A2-%C3%A2
#17
Elizabeth M Snyder, Konstantin Licht, Robert E Braun
Adenosine to inosine (A-to-I) RNA editing occurs in a wide range of tissues and cell types and can be catalyzed by one of the two adenosine deaminase acting on double-stranded RNA enzymes, ADAR and ADARB1. Editing can impact both coding and noncoding regions of RNA, and in higher organisms has been proposed to function in adaptive evolution. Neither the prevalence of A-to-I editing nor the role of either ADAR or ADARB1 has been examined in the context of germ cell development in mammals. Computational analysis of whole testis and cell-type specific RNA-sequencing data followed by molecular confirmation demonstrated that A-to-I RNA editing occurs in both the germ line and in somatic Sertoli cells in two targets, Cog3 and Rpa1...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28364521/rna-immunoprecipitation-identifies-novel-targets-of-dazl-in-human-foetal-ovary
#18
Roseanne Rosario, Richard W P Smith, Ian R Adams, Richard A Anderson
Study question: Can novel meiotic RNA targets of DAZL (deleted in azoospermia-like) be identified in the human foetal ovary? Summary answer: SYCP1 (synaptonemal complex protein-1), TEX11 (testis expressed 11) and SMC1B (structural maintenance of chromosomes 1B) are novel DAZL targets in the human foetal ovary, thus DAZL may have previously unrecognised roles in the translational regulation of RNAs involved in chromosome cohesion and DNA recombination in the oocyte from the time of initiation of meiosis...
March 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28298247/tissue-specific-inhibition-of-urokinase-type-plasminogen-activator-expression-in-the-testes-of-mice-by-inducible-lentiviral-rna-interference-causes-male-infertility
#19
Kai Zhao, Yan Liu, Zhe Xiong, Lian Hu, Cheng-Liang Xiong
Urokinase-type plasminogen activator (uPA) is involved in many physiological processes, including male infertility. To explore the effects of uPA in male reproduction, we constructed an inducible uPA short hairpin RNA (shRNA) system expressed by lentiviral vectors. After proving inhibition of uPA expression in the mouse Sertoli cell line TM4 by 1μgmL-1 doxycycline (Dox), two lentivirus (pLenti4-shRNA and pLenti6/TR) were co-microinjected into mouse testes to produce TetR&shuPA mice model. Though oral gavage by 0...
March 16, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28291811/systematic-identification-and-characterization-of-long-non-coding-rnas-in-mouse-mature-sperm
#20
Xiaoning Zhang, Fengxin Gao, Jianbo Fu, Peng Zhang, Yuqing Wang, Xuhui Zeng
Increasing studies have shown that mature spermatozoa contain many transcripts including mRNAs and miRNAs. However, the expression profile of long non-coding RNAs (lncRNAs) in mammalian sperm has not been systematically investigated. Here, we used highly purified RNA to investigate lncRNA expression profiles in mouse mature sperm by stranded-specific RNA-seq. We identified 20,907 known and 4,088 novel lncRNAs transcripts, and the existence of intact lncRNAs was confirmed by RT-PCR and fluorescence in situ hybridization on two representative lncRNAs...
2017: PloS One
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