keyword
https://read.qxmd.com/read/38351456/data-analysis-pipeline-for-scrna-seq-experiments-to-study-early-oogenesis
#21
JOURNAL ARTICLE
Wei Ge, Teng Zhang, Yang Zhou, Wei Shen
Germ cells as the means for the transmission of genetic information between generations have been a hot topic of research for decades. The analysis of the transcriptomes, that is of the RNA transcripts produced by the genotype at a given time, of germ cells and the surrounding somatic cells, is essential to unravel the cellular and molecular processes regulating gametogenesis. However, the asynchronized differentiation of germ cells and high cellular heterogeneity in the developing ovary or testis represent two unsurmountable challenges for delineating the transcription regulation mechanism of germ cells using traditional bulk RNA sequencing...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38351446/isolation-of-mouse-germ-cells-by-facs-using-hoechst-33342-and-syto16-double-staining
#22
JOURNAL ARTICLE
Mark E Gill, Hubertus Kohler, Antoine H F M Peters
In the adult mouse testis, germ cells of various developmental cell states co-exist. FACS isolation of cells stained with the DNA dye Hoechst 33342 has been used for many years to sub-divide these cells based on their DNA content. This approach provides an efficient way to obtain broad categories of male germ cells: pre-meiotic spermatogonia, meiotic spermatocytes and post-meiotic spermatids. The addition of a red filter for Hoechst staining enables further sub-division of spermatocytes depending on sub-stages of meiotic prophase...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38349859/single-cell-transcriptomics-identifies-senescence-associated-secretory-phenotype-sasp-features-of-testicular-aging-in-human
#23
JOURNAL ARTICLE
Junxian He, Jindong Li, Yanqing Li, Zhenhan Xu, Menghui Ma, Haicheng Chen, Peigen Chen, Linyan Lv, Xuejun Shang, Guihua Liu
The male reproductive system experiences degradation with age, predominantly impacting the testes. Testicular aging can result in failure to produce physiological testosterone levels, normal sperm concentrations, or both. However, we cannot predict the onset of testicular aging in advance. Using single-cell RNA sequencing (scRNA-seq) from Gene Expression Omnibus (GEO) database, we conducted cell-cell communication network of human testis between older and young group, indicating Leydig cells' potential role in spermatogenesis microenvironment of aging testis...
February 12, 2024: Aging
https://read.qxmd.com/read/38341879/1-2-dichloroethane-induces-testicular-pyroptosis-by-activating-pir-mmu-1019957-irf7-pathway-and-the-protective-effects-of-melatonin
#24
JOURNAL ARTICLE
Bingli Zhang, Yizhou Zhong, Jiaxin Du, Rongyi Ye, Bingchi Fan, Yanhong Deng, Ruobing Bai, Yu Feng, Xiaohong Yang, Yuji Huang, Boxuan Liang, Jiewei Zheng, Weifeng Rong, Xingfen Yang, Zhenlie Huang
1,2-Dichloroethane (1,2-DCE) is a prevalent environmental contaminant, and our study revealed its induction of testicular toxicity in mice upon subacute exposure. Melatonin, a prominent secretory product of the pineal gland, has been shown to offer protection against pyroptosis in male reproductive toxicity. However, the exact mechanism underlying 1,2-DCE-induced testicular toxicity and the comprehensive extent of melatonin's protective effects in this regard remain largely unexplored. Therefore, we sequenced testis piRNAs in mice exposed to environmentally relevant concentrations of 1,2-DCE by 28-day dynamic inhalation, and investigated the role of key piRNAs using GC-2 spd cells...
February 9, 2024: Environment International
https://read.qxmd.com/read/38341078/5-methylcytidine-effectively-improves-spermatogenesis-recovery-in-busulfan-induced-oligoasthenospermia-mice
#25
JOURNAL ARTICLE
Chengniu Wang, Taowen Ye, Junze Bao, Jin Dong, Wenran Wang, Chunhong Li, Hongping Ding, Hanqing Chen, Xiaorong Wang, Jianwu Shi
The function and regulatory mechanisms of 5-methylcytidine (m5 C) in oligoasthenospermia remain unclear. In this study, we made a mouse model of oligoasthenospermia through the administration of busulfan (BUS). For the first time, we demonstrated that m5 C levels decreased in oligoasthenospermia. The m5 C levels were upregulated through the treatments of 5-methylcytidine. The testicular morphology and sperm concentrations were improved via upregulating m5 C. The cytoskeletal regenerations of testis and sperm were accompanying with m5 C treatments...
February 8, 2024: European Journal of Pharmacology
https://read.qxmd.com/read/38328171/multifaceted-roles-of-histone-lysine-lactylation-in-meiotic-gene-dynamics-and-recombination
#26
Xiaoyu Zhang, Yan Liu, Ning Wang
Male germ cells, which are responsible for producing millions of genetically diverse sperm through meiosis in the testis, rely on lactate as their central energy metabolite. Recent study has revealed that lactate induces epigenetic modification in cells through histone lactylation, a post-translational modification involving the addition of lactyl groups to lysine residues on histones. Here we report dynamic histone lactylation at histone H4-lysine 5 (K5), -K8, and -K12 during meiosis prophase I in mouse spermatogenesis...
January 26, 2024: bioRxiv
https://read.qxmd.com/read/38320369/ursonic-acid-attenuates-spermatogenesis-in-oligozoospermia-mice-through-inhibiting-ferroptosis
#27
JOURNAL ARTICLE
Jin Dong, Yanli Dong, Hanqing Chen, Taowen Ye, Guangtong Chen, Boyi Fan, Xiaorong Wang, Jianwu Shi, Chengniu Wang
Ursonic acid (UNA) is a natural pentacyclic triterpene found in some medicinal plants and foods. The reproductive protective effect of UNA was evaluated in a mouse model of oligozoospermia induced by busulfan (BUS) at 30 mg/kg b.w.. The mice were initially divided into groups with UNA concentrations of 10, 30, 50, 100 mg/kg. Subsequently, based on sperm parameters, the optimal concentration of 50 mg/kg was identified. As a control, an additional group was supplemented with ursolic acid at a concentration of 50 mg/kg...
February 3, 2024: Bioorganic Chemistry
https://read.qxmd.com/read/38307907/single-cell-map-of-human-azoospermia-testis-caused-by-cyclophosphamide-chemotherapy
#28
JOURNAL ARTICLE
Jian Cao, Xueheng Zhao, Zailong Qin, Shanshan Lv, Lin Du, Zhizhong Liu, Liqing Fan, Hao Bo
Chemotherapeutic drugs will affect the process of spermatogenesis. However, most current studies on the effects of chemotherapeutic drugs on spermatogenesis are based on mouse models, with a shortage of human body evidence. In addition, the mechanism of chemotherapeutic drugs causing spermatogenesis disorder is not clear. Therefore, we have collected the testicular tissues of an inguinal-lipoma patient whose testes were resected after chemotherapy and a patient who had normal spermatogenesis disorder and underwent single-nucleus RNA sequencing (snRNA-Seq)...
February 2, 2024: Scientific Data
https://read.qxmd.com/read/38301068/iron-regulatory-proteins-1-and-2-have-opposing-roles-in-regulating-inflammation-in-bacterial-orchitis
#29
JOURNAL ARTICLE
Niraj Ghatpande, Aileen Harrer, Bar Azoulay-Botzer, Noga Guttmann-Raviv, Sudhanshu Bhushan, Andreas Meinhardt, Esther G Meyron-Holtz
Acute bacterial orchitis (AO) is a prevalent cause of intra-scrotal inflammation, often resulting in sub- or infertility. A frequent cause eliciting AO is uropathogenic Escherichia coli (UPEC), a gram negative pathovar, characterized by the expression of various iron acquisition systems to survive in a low-iron environment. On the host side, iron is tightly regulated by iron regulatory proteins (IRP) 1 and 2 and these factors are reported to play a role in testicular and immune cell function, however, their precise role remains unclear...
February 1, 2024: JCI Insight
https://read.qxmd.com/read/38292234/partial-lipectomy-of-the-epididymal-fat-alters-expression-of-the-steroidogenic-enzymes-in-the-mouse-testis-at-different-postnatal-ages
#30
JOURNAL ARTICLE
Yong-Seung Lee, Ki-Ho Lee
The epididymal fat is a type of gonadal adipose tissue, which is localized closely to the testis. Even though it has been suggested that the epididymal fat is necessary for maintenance of spermatogenesis in the testis, the influence of epididymal fat on expression of testicular steroidogenic enzymes has not been examined. In the present research, expressional changes of steroidogenic enzymes in the mouse testis after 2 weeks of the surgical partial lipectomy of epididymal fat at different postnatal ages were determined by real-time polymerase chain reaction analysis...
December 2023: Balsaeng'gwa Saengsig
https://read.qxmd.com/read/38291311/profiling-of-testis-specific-or-testis-predominant-genes-expressed-in-mouse-male-germ-cell-lines-gc-1-and-gc-2
#31
JOURNAL ARTICLE
Seungho Choi, Seong Hyeon Hong, Gwidong Han, Chunghee Cho
BACKGROUND: Spermatogenesis is a tightly organized process that utilizes an intrinsic genetic program composed of germ cell-specific genes. Although mouse germ cell-related cell lines are available, few germ cell-specific genes have been comprehensively identified in such cell lines. OBJECTIVE: We aimed to profile gene expression in the male mouse germ cell-related cell lines, GC-1 and GC-2, characterize their transcriptomic nature, and identify potential testis- or germ cell-specific or -predominant genes expressed in these cell lines...
January 30, 2024: Genes & Genomics
https://read.qxmd.com/read/38284772/cuproptosis-mediates-copper-induced-testicular-spermatogenic-cell-death
#32
JOURNAL ARTICLE
Jing-Yi Zhang, Xu-Jun Yu, Jun-Jun Li, Yao Xiao, Guang-Sen Li, Fang Yang, Liang Dong
Cuproptosis, a novel mechanism of programmed cell death, has not been fully explored in the context of spermatogenic cells. This study investigated the potential involvement of cuproptosis in spermatogenic cell death using a mouse model of copper overload. Sixty male Institute of Cancer Research (ICR) mice were randomly divided into four groups that received daily oral gavage with sodium chloride (control) or copper sulfate (CuSO4) at 50 mg kg-1, 100 mg kg-1, or 200 mg kg-1, for 42 consecutive days. Mice subjected to copper overload exhibited a disruption in copper homeostasis...
January 26, 2024: Asian Journal of Andrology
https://read.qxmd.com/read/38284481/balanced-spatiotemporal-arrangements-of-histone-h3-and-h4-posttranslational-modifications-are-necessary-for-meiotic-prophase-i-chromosome-organization
#33
JOURNAL ARTICLE
S Lava Kumar, Aradhana Mohanty, Anjali Kumari, Ajith Kumar Etikuppam, Ranjith Kumar S, Mohd Athar, Kiran Kumar P, Rohit Beniwal, Moukthika M Potula, Ravi Kumar Gandham, H B D Prasada Rao
Dynamic nuclear architecture and chromatin organizations are the key features of the mid-prophase I in mammalian meiosis. The chromatin undergoes major changes, including meiosis-specific spatiotemporal arrangements and remodeling, the establishment of chromatin loop-axis structure, pairing, and crossing over between homologous chromosomes, any deficiencies in these events may induce genome instability, subsequently leading to failure to produce gametes and infertility. Despite the significance of chromatin structure, little is known about the location of chromatin marks and the necessity of their balance during meiosis prophase I...
January 29, 2024: Journal of Cellular Physiology
https://read.qxmd.com/read/38284221/three-dimensional-cell-culture-of-epididymal-basal-cells-and-organoids-a-novel-tool-for-toxicology
#34
JOURNAL ARTICLE
Julie Dufresne, Mary Gregory, Laurie Pinel, Daniel G Cyr
Spermatozoa are formed in the testis but must transit through the epididymis to acquire motility and the ability to fertilize. The epididymis is a single convoluted tubule comprising several anatomically and physiologically distinct regions. The pseudostratified epithelium consists of multiple cell types, including principal cells, clear cells, narrow cells, and apical cells, that line the lumen of the epididymis. Basal cells are present at the base of the epithelium, and halo cells, which includes macrophages/monocytes, mononuclear phagocytes, and T lymphocytes, are also present in the epithelium...
January 2024: Current protocols
https://read.qxmd.com/read/38281153/intronless-pabpc6-encodes-a-testis-specific-cytoplasmic-poly-a-binding-protein-but-is-dispensable-for-spermatogenesis-in-the-mouse
#35
JOURNAL ARTICLE
Yuko Kaku, Yuka Isono, Hideto Tanaka, Tomohiro Kobayashi, Yoshinori Kanemori, Shin-Ichi Kashiwabara
Besides ubiquitous poly(A)-binding protein, cytoplasmic 1 (PABPC1), testis-specific PABPC2/PABPt (in humans, referred to as PABPC3), and female and male germline-specific PABPC1L/ePAB, have been reported in the mouse testis. Recent in silico analysis additionally identified testis-specific Pabpc6 in the mouse. In this study, we characterized PABPC6 and its mutant mice. PABPC6 was initially detectable in the cytoplasm of pachytene spermatocytes, increased in abundance in round spermatids, and decreased in elongating spermatids...
January 28, 2024: Biology of Reproduction
https://read.qxmd.com/read/38280889/postnatal-development-of-mouse-spermatogonial-stem-cells-as-determined-by-immunophenotype-regenerative-capacity-and-long-term-culture-initiating-ability-a-model-for-practical-applications
#36
JOURNAL ARTICLE
Youngmin Song, Xiangfan Zhang, Joëlle A Desmarais, Makoto Nagano
Spermatogonial stem cells (SSCs) are the foundation of life-long spermatogenesis. While SSC research has advanced greatly over the past two decades, characterization of SSCs during postnatal development has not been well documented. Using the mouse as a model, in this study, we defined the immunophenotypic profiles of testis cells during the course of postnatal development using multi-parameter flow cytometry with up to five cell-surface antigens. We found that the profiles progress over time in a manner specific to developmental stages...
January 27, 2024: Scientific Reports
https://read.qxmd.com/read/38267037/establishment-of-an-organ-culture-system-to-maintain-the-structure-of-mouse-m%C3%A3-llerian-ducts-during-development
#37
JOURNAL ARTICLE
Shiori Kato, Toshifumi Yokoyama, Taisei Fujikawa, Yusuke Kirizuki, Youhei Mantani, Takanori Miki, Nobuhiko Hoshi
We previously showed that the anti-Müllerian hormone (AMH), infiltrating from the testis to the mesonephros reaches the cranial and middle regions of the Müllerian duct (MD) and induces their regression using an organ culture in mice. However, it is difficult to maintain structural integrity, such as the length and diameter and normal direction of elongation of the caudal region of the MD, in conventional organ culture systems. Therefore, the pathway of AMH to the caudal MD region remains uncharted...
January 24, 2024: Journal of Veterinary Medical Science
https://read.qxmd.com/read/38262615/a-novel-alternative-method-for-long-term-evaluation-of-male-reproductive-toxicity-and-its-recovery-using-a-pre-pubertal-mouse-testis-organ-culture-system
#38
JOURNAL ARTICLE
Kiyoshi Hashimoto, Hiroshi Arakawa, Rikako Imamura, Takuya Nishimura, Satoshi Kitajima, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa, Satoshi Yokota
Successful treatment of pediatric cancers often results in long-term health complications, including potential effects on fertility. Therefore, assessing the male reproductive toxicity of anti-cancer drug treatments and the potential for recovery is of paramount importance. However, in vivo evaluations are time-intensive and require large numbers of animals. To overcome these constraints, we utilized an innovative organ culture system that supports long-term spermatogenesis by placing the testis tissue between a base agarose gel and a polydimethylsiloxane ceiling, effectively mirroring the in vivo testicular environment...
January 23, 2024: Journal of Applied Toxicology: JAT
https://read.qxmd.com/read/38262568/a-novel-antigenic-epitope-identified-on-the-accessory-protein-ns6-of-porcine-deltacoronavirus
#39
JOURNAL ARTICLE
Qiuge Liu, Jianxiao Wu, Na Gao, Xiaorong Zhang, Mingzhe Gao, Xin Zhang, Longjun Guo, Yang Wu, Da Shi, Hongyan Shi, Jianfei Chen, Li Feng
Porcine deltacoronavirus (PDCoV) is a novel enteric coronavirus that can cause vomiting, watery diarrhea in pigs and the death of piglets. The open reading frame (ORF) 5 is one of the accessory genes in PDCoV genome and encodes an accessory protein NS6. To date, the function of NS6 is still unclear. In this study, the recombinant NS6 was successfully expressed in prokaryotic expression system and purified. To prepare monoclonal antibody (mAb), six-week-old female BALB/c mice were primed subcutaneously with purified NS6...
January 21, 2024: Virus Research
https://read.qxmd.com/read/38250158/towards-a-testis-in-a-dish-generation-of-mouse-testicular-organoids-that-recapitulate-testis-structure-and-expression-profiles
#40
JOURNAL ARTICLE
Aviya Stopel, Cheli Lev, Stav Dahari, Or Adibi, Leah Armon, Nitzan Gonen
The testis is responsible for sperm production and androgen synthesis. Abnormalities in testis development and function lead to disorders of sex development and male infertility. Currently, no in vitro system exists for modelling the testis. Here, we generated testis organoids from neonatal mouse primary testicular cells using transwell inserts and show that these organoids generate tubule-like structures and cellular organization resembling that of the in vivo testis. Gene expression analysis of organoids demonstrates a profile that recapitulates that observed in in vivo testis...
2024: International Journal of Biological Sciences
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